ANALYTICAL BIOCHEMISTRY
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2024
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IF 2.6
Lin Tian,Yang Li,Huiqing Wang,Xinyi Li,Qian Gao,Yaru Liu,Yao Liu,Qing Wang,Cuiping Ma,Chao Shi
Vibrio parahaemolyticus ( V. parahaemolyticus ) is a major foodborne pathogen , which can cause serious foodborne illnesses like diarrhoea. Rapid on-site detection of foodborne pathogens is an ideal way to respond to foodborne illnesses. Herein, we provide an electrochemical sensor for rapid on-site detection. This sensor utilized a pH-sensitive metal-oxide material for the concurrent isothermal amplification and label-free detection of nucleic acids . Based on a pH-sensitive hydrated iridium oxide oxyhydroxide film (HIROF), the electrode transforms the hydrogen ion compound generated during nucleic acid amplification into potential, so as to achieve a real-time detection. The results can be transmitted to a smartphone via Bluetooth. Moreover, HIROF was applied in nucleic acid device detection, with a super-Nernst sensitivity of 77.6 mV/pH in the pH range of 6.0–8.5, and the sensitivity showed the best results so far. Detection of V. parahaemolyticus by this novel method showed a detection limit of 1.0 × 10 3 CFU/mL, while the time consumption was only 30 min, outperforming real-time fluorescence loop-mediated isothermal amplification (LAMP). Therefore, the characteristics of compact, portable, and fast make the sensor more widely used in on-site detection.
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DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2021
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IF 3.575
Chengyu Zhang,Lin Zhu,Jiaxing Zhang,Wenhang Wang,Yan Zeng,Shengping You,Wei Qi,Rongxin Su,Zhimin He
In this study, we aimed to develop B. subtilis spore coat protein A (CotA) for the enzymatic determination of bilirubin . Firstly, molecular docking and oxidation kinetic analysis confirmed the feasibility of CotA for oxidizing bilirubin . Secondly, CotA showed pH-preferable oxidization performance to direct bilirubin (DB) in acidic conditions and an alkaline-catalytic oxidation capacity to total bilirubin (TB). Mechanism analysis results confirm that the conformational changes of CotA, DB and UB caused by pH changes are responsible for the selective oxidation of DB and TB by CotA. Then, CotA exhibits better structural characteristics and enzymatic performance than M. verrucaria -derived bilirubin oxidase ( Mv -BOD). Besides, the strong anti-interference ability helps CotA adapt to complex catalytic environment in the detection of DB and TB. Our results prove that CotA can be used as a promising candidate bio-enzymatic detection reagent for DB and TB.
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DOI
高影响因子
CERAMICS INTERNATIONAL
·
2020
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IF 3.83
Xinqiang Cao,Yan Gu,Hailin Tian,Yanfen Fang,David Johnson,Zhiyong Ren,Chuncheng Chen,Yingping Huang
In this work, BiVO 4 composites, containing the tetragonal zircon phase ( tz -BiVO 4 ), and monoclinic scheelite phase ( ms -BiVO 4 ), were synthesized using the microemulsion method. The effect of pH on phase composition and photocatalytic activity were investigated. Based on X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), a ms/tz -BiVO 4 composite forms at pH = 1.0 and pure ms -BiVO 4 is obtained in the pH range 4.0–10.0. The three primary steps in preparing BiVO 4 were monitored by optical microscopy and the role played by the microemulsion on the phase composition of BiVO 4 is explained. Photoluminescence spectroscopy (PL), UV–visible diffuse reflectance spectroscopy (UV-DRS), Brunauer-Emmett-Teller (BET), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were employed to characterize the physical and chemical properties of BiVO 4 composites. The composite formed at pH = 1 exhibited the lowest hole-electron ( h + - e - ) recombination rate, resulting in the highest photocatalytic activity towards microcystin-LR (MC-LR), with near 100% removal of MC-LR in 5 h. ESR and trapping experiments indicated that MC-LR degradation was mediated primarily by hydroxyl radicals (•OH), superoxide radicals (O 2 •− ) and photogenerated holes ( h + ).
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DOI
高影响因子
Advanced Powder Materials
·
2022
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IF 0
Hailing Yang,Zhi Wang,Mengyong Sun,Fei Chen,Jun Ji,Sique Chen,Yingying Chen,Deli Ma,Zhenyu Zhang,Baocai Pan,Yulin Wei,Qinggang Li
As a non-toxic copolymer of isobutylene and maleic anhydride, Isobam is successfully used as a dispersant and a gelling agent for fabricating porous Si 3 N 4 ceramics by gel casting. The dispersity and rheological properties of the Si 3 N 4 slurry are influenced by the pH, milling time, and Isobam content which varies from 0.1 wt.% to 0.6 wt.%, and these factors are investigated. The slurry with 40 vol.% solid content and milled for 4 h has a high zeta potential at pH 12 (adjusted by Tetramethyl ammonium hydroxide (TMAH)), which means that the particles are well dispersed. The mechanisms of TMAH are electrostatic repulsion and steric hindrance. The viscosity of the slurry increases with the increase of Isobam content. After pressureless sintering at 1700 °C for 2 h, a uniform unique interlocking microstructure of rod-like β-Si 3 N 4 grains is observed, which may improve the flexural strength of the ceramics by intergranular fracture and particle pullout of β-Si 3 N 4 grains. The density and porosity of the samples fluctuate negligibly with the increase of Isobam content, and the Si 3 N 4 ceramic with 0.1 wt.% Isobam exhibits the highest bending strength of 251.6 MPa among all samples.
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DOI
LANGMUIR
·
2024
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IF 3.7
Jiawei Liu,Guo Du,Tiehong Chen
Ordered mesoporous silica is widely used in catalysis, adsorption, and biomedicine, among which SBA-15 (Santa Barbara Amorphous-15) is one of the most widely studied. However, the synthesis of SBA-15 often requires strong acid (hydrochloric acid or sulfuric acid), which will not only corrode industrial equipment but also pollute the environment with the wastewater containing strong acid and halogen (sulfur). Here, we demonstrate a green synthetic strategy for SBA-15 under weakly acidic conditions through an anionic assembly route. With the assistance of poly(acrylic acid) (PAA) and 3-aminopropyltrimethoxysilane (APMS), the pH value of the synthesis system can be increased to 4–5, which is a mild near-neutral condition. In addition, halogen-free synthesis using organic acids is also achieved. The powder X-ray diffraction (XRD), transmission electron microscopy (TEM), and N2 sorption characterizations show that the obtained SBA-15 has good texture properties, with a specific surface area of 430–500 m2/g and ordered 6–8 nm mesopores, which is similar to SBA-15 synthesized in traditional strong acid. This strategy provides a facile and environmentally friendly route for the large-scale production of ordered mesoporous materials.
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DOI
高影响因子
International Journal of Nanomedicine
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2020
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IF 5.115
Li He,Fangzhen Qing,Maode Li,Daitian Lan
Purpose The aim of this study was to develop an “all-in-one” nanoplatform that integrates at the second near-infrared (NIR-II) region dye IR1061 and anticancer drug paclitaxel (PTX) into an apoferritin (AFN) nanocage (IR-AFN@PTX). Simultaneously, folic acid (FA), tumor target molecule, was conjugated onto IR-AFN@PTX to be IR-AFN@PTX-FA for tumor-targeted and pH/NIR-II-triggered synergistic photothermal-chemotherapy.Methods IR1061 was firstly reacted with PEG and then conjugated with AFN to be IR-AFN. Then, FA was conjugated onto the surface of IR-AFN to be IR-AFN-FA. At last, PTX was incorporated into IR-AFN-FA to fabricate a nanoplatform IR-AFN@PTX-FA. The NIR-II photothermal properties and pH/NIR-II triggered drug release were evaluated. The ability of IR-AFN@PTX-FA to target tumors was estimated using optical bioluminescence. In vitro and in vivo synergistic therapeutic effects of pH/NIR-II-triggered and tumor-targeted photothermal-chemotherapy were investigated in 4T1 tumor model.Results IR-AFN@PTX-FA showed excellent water solubility and physiological stability, which significantly enhanced the solubility of both IR1061 and PTX. After 5 min of laser irradiation at 1064 nm, IR-AFN@PTX-FA exhibited an effective photothermal effect compared with laser irradiation at 808 nm, even when blocked with 0.6 cm thick chicken breast. Cellular uptake experiments showed IR-AFN@PTX-FA utilized clathrin-mediated and caveolae-mediated endocytosis pathways to enter 4T1 cells, and was then delivered by the endosome to the lysosome. NIR-II laser irradiation and pH could synergistically trigger PTX release, inducing significant tumor inhibition in vitro and in vivo.Conclusion As a novel “all-in-one” nanoplatform, IR-AFN@PTX-FA was found to selectively target tumors and showed very efficient NIR-II photothermal effects and pH/NIR-II triggered drug release effects, showing a remarkable, synergistic photothermal-chemotherapy effect.
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Shasha Li,Xunzhao Qiu,Xiaowei An,Enze Li,Xueyan Li,Guiru Wang,Peng Li,Caixia Shi,Yiming Liu,Guoqing Guan
Metal-organic frameworks (MOFs) derived spinel tricobalt tetroxide (Co 3 O 4 ) electrocatalysts incorporated with trifle Iridium (Ir) sites are in-situ grown on nickel foam (NF) substrate (Ir-Co 3 O 4 /NF) as effective anode for OER especially in near-pH-neutral seawater with high ability to suppress chlorine evolution reaction. Density functional theory (DFT) calculations indicate that the trifle Ir sites can be safeguarded in spinel Co 3 O 4 via structural-confinement and charge-redistribution so that the Ir and Co sites with their bridged electrophilic O ligands can serve as the active sites for boosting OER performance. As expected, a relatively low OER overpotential of 370 mV @ 10 mA cm −2 and it is achieved with at least 7 days durability under the current density of 100 mA cm −2 in near-pH-neutral simulated seawater with high selectivity toward OER. In same condition, a fabricated electrolyzer using it presents excellent electrocatalytic performance with a cell voltage of 2.11 V @ 100 mA cm −2 , strongly suggesting that the present electrolyzer has great promising for practical seawater electrolysis.
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DOI
高影响因子
POLYMER
·
2015
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IF 3.562
Lin Wang,Yin Wang,Qiao Jin,Fan Jia,Haibo Wang,Jian Ji
Biomimic hyaluronic acid (HA) prodrug micelles with pH and reduction responsiveness were prepared by chemical graft of phosphorylcholine and doxorubicin (DOX) to the backbone of HA. DOX was conjugated to HA by pH-sensitive hydrazone bonds which could be hydrolyzed under acidic conditions and thus resulted in controlled release of DOX. Besides, the HA prodrug micelles were cross-linked via reduction-responsive disulfide bonds to improve the stability of the micelles. The HA prodrug can form remarkable core–shell micelles with a quite narrow size distribution which was confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The in vitro drug release studies showed that there was a dramatic release under endosome pH (pH = 5.0) and reductive environment (10 mM of dithiothreitol) than that at normal physiological environment (pH = 7.4). Greater uptake of DOX from HA prodrug micelles was observed in the CD44 receptor highly expressed MDA-MB-231 cell line, compared to the results from the CD44-negative cell line, NIH3T3, which was demonstrated by the flow cytometry and fluorescence microscopy . The glutathione (GSH)-responsive behaviors were studied as well. The micelles showed higher cellular proliferation inhibition against glutathione monoester (GSH-OEt) pretreated MDA-MB-231 cells than that of the non-pretreated and buthionine sulfoximine (BSO) pretreated ones. These results suggested that such pH/reduction dual-responsive prodrug micelles may enhance the selective inhibition to cancer cells which exhibit higher levels of GSH concentration and provide favorable platforms for cancer therapy.
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
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IF 8.3
Ziyue Peng,Yuqi Du,Mingrui Song,Chongkai Sun,Yuan Xu,Jikun Qian,Xin Guan,Mankai Yang,Xin Zhang,Fangfang Dai,Haoyang Wan,Yuhang Chen,Chun Liu,Bin Yu
Implant-associated infections frequently complicate orthopedic surgeries, resulting in challenging issues. The current therapy of antibiotic treatment and surgical debridement often leads to drug resistance and bone defect. The development of pH-responsive antimicrobial and pro-osteogenic materials is a promising approach to controlling infections and repairing infected bone defects, especially given the weakly acidic pH of the bacterial infection area. Solid peroxides have the potential to provide a sustained release of hydrogen peroxide (H2O2), rendering them applicable for antimicrobial purposes. Additionally, their chemical properties render them inherently responsive to pH. Here, we propose a novel GelBA/PVA/MgO2 hydrogel composed of gelatin (Gel), benzeneboronic acid (BA), poly(vinyl alcohol) (PVA), and magnesium peroxide (MgO2) with self-healing ability and pH-responsiveness. The borate ester bond formed between PVA and BA is a dynamic chemical bond with properties of dynamic formation and dissociation, making the hydrogel both self-healable and pH-responsive. Meanwhile, the addition of MgO2 improves the network structure of the hydrogel and gives the hydrogel the ability to perform sustained release of H2O2 and Mg2+. Experimentally, the GelBA/PVA/MgO2 hydrogel exhibits controlled and pH-dependent H2O2 and Mg2+ release, sustained over time at physiological pH (7.4) and significantly increased at infection pH (5.5). In vitro and in vivo outcomes revealed that this hydrogel is able to inhibit Staphylococcus aureus growth and accelerate bone regeneration, improving bone healing without cytotoxic effects on normal tissues. These findings suggest that the GelBA/PVA/MgO2 hydrogel is a unique and efficient approach for anti-infection and therapeutic implant-associated infections.
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Hailong Ma,Zhiqiang Luo,Jiale Ding,Dan Zhang,Feiyi Wang,Huili Yu,Wei Chen,Jun Ren,Erfei Wang
Accurate tumor diagnosis and drug efficacy assessment are crucial aspects of cancer treatment. Tumor cells typically display aberrant levels of biothiols, higher viscosity, and lower acidity compared to normal cells. Thus, precise monitoring of biothiol levels in the intricate cellular environment is essential for early tumor detection. We have developed a novel near-infrared (NIR) fluorescence probe AX-DNBS for the simultaneous detection of biothiols, viscosity, and pH through multi-channel imaging. With the treatment of biothiol, AX-DNBS triggers the release of AX-OH, resulting in enhanced fluorescence emission at 710 nm. Both AX-DNBS and AX-OH exhibit sensitive responses to changes in viscosity, with fluorescence emissions at 475 nm and 640 nm, respectively. In the pH range of 3–10, an increase in alkalinity leads to a ratiometric fluorescence signal of AX-OH with a significant emission red-shift from 640 nm to 710 nm. Significantly, in subsequent biological trials, AX-DNBS proved effective in monitoring alterations in biothiol levels, viscosity, and pH within live cells, facilitating the diagnosis of tumors and the evaluation of the therapeutic effects of paclitaxel in tumor mice models.
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DOI
高影响因子
Fundamental Research
·
2022
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IF 0
Hongguang Liao,Yanji Chu,Shenglong Liao,Yonglin He,Yingchao Ma,Yapei Wang
Photothermal therapy has aroused great attention and showed promising potential in minimally invasive tumor ablation, but the clinical translation is still stifled by the concerns of unwanted injury to normal tissues. The safety concerns might be completely solved only when the two security obstacles of “material-toxicity” and “photo-toxicity” were overcome simultaneously. Herein, a completely non-toxic food-grade photothermal transduction agent (PTA) with double safety guarantees was invented, which shows an absolute transformation of the photothermal effect from “0” to “1” after being triggered by an acidic tumor microenvironment. Inspired by the classical starch-iodine test, a preprogrammed [starch-KI-KIO 3 ] complex was prepared in large quantities through a modified wet-milling procedure. It's demonstrated that a macroscopic consecutive reaction could be triggered by low pH to produce the starch-iodine complex which can generate lethal temperature under the near-infrared light irradiation. Meanwhile, the PTA shows excellent biocompatibility with no “material-toxicity” owing to the raw materials drawn from our daily food. Animal experiments reveal that the tumor microenvironment can activate the switch of photothermal effect from “0” to “1” successfully, which is thus responsible for the discriminative photo-damage to the tumor region while no “photo-toxicity” to normal tissue. The good treatment efficacy confirms the feasibility of such photothermal transduction agents with double safety guarantees in clinical applications.
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DOI
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2022
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IF 5.062
Junxu Hao,Jinglei Wang,Hao Pan,Yuli Sang,Dazhuang Wang,Zeyu Wang,Jiao Ai,Bin Lin,Lijiang Chen
In this study, the ideal polymer materials were selected by molecular dynamics (MD) and dissipative particle dynamics (DPD) simulation methods, and pH and redox-sensitive micelles were prepared according to the simulation results. Through experiments, we successfully prepared hydrophilic block polyethylene glycol monomethyl ether (mPEG) connected hydrophobic block polylactic acid-glycolic acid (PLGA) amphiphilic polymer, and connected with redox fragment containing disulfide bond , and then connected with amide bond doxorubicin (DOX) to form mPEG-PLGA-SS-DOX prodrug , and further physical embedded free DOX. Finally, we obtained a particle size of 47.04 ± 0.103 nm, the potential of −23.8 ± 0.189 mV, Polymer dispersity index (PDI) of 0.231 ± 0.001, transmission electron microscopy (TEM) showed that it was spherical mPEG-PLGA-SS-DOX/DOX nanoparticles , its entrapment efficiency (EE) was 68.2%, loading content was 16.72%. The cumulative release of mPEG-PLGA-SS-DOX/DOX was 87.39% in a pH 5.0 and 10 mM Glutathione (GSH) tumor simulation environment. It was less toxic to normal cell HEK293 and more cytotoxic to HepG2 tumor cells. It showed that it was an excellent anti-tumor nano-drug. In addition, the application of computer simulation in the design of nanomedicine also provided a new research idea for the research of nanomedicine .
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高影响因子
危险品化学品经营许可证（带存储）