BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 40
·期 1
·e70278
·
IF 1.7
Junni Fan,Yu Li,Ge Wang,Qian Li,Minfeng Fang
This study employed an affinity chromatography approach to investigate the potential molecular mechanism of the Albiziae Cortex and Polygoni Multiflori Caulis herbal pair, which is commonly used for treating insomnia. Briefly, the haloalkane dehalogenase and its substrate were fused separately onto the serotonin 1A receptor and amino microspheres. Receptors were bound onto chromatographic stationary phases specifically through biological orthogonal interactions. Subsequently, catechin, methyl-O-digalloyl-hexose, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), and julibroside J 35 were identified as active components in the herbal pair extract targeting the receptor. A mice model of insomnia was used to validate the anti-insomnia effects. The herbal-pair extract, catechin, and THSG significantly reduced sleep latency and increased sleep duration. Furthermore, on-column analysis and molecular docking revealed the binding characteristic. Adsorption isotherm analysis indicated a homogeneous binding process, which was further supported by injection amount-dependent analysis. The latter also provided binding constants of (5.70 ± 0.61) × 10 5 M −1 for catechin and (4.05 ± 0.20) × 10 5 M −1 for THSG. Molecular docking revealed critical binding residues (Asp116, Val117, Thr121, Lys191, Thr196, and Ser199), primarily via van der Waals forces and hydrogen bonds. Collectively, immobilizing the serotonin 1A receptor enables the screening of biologically active compounds from complex systems effectively and shows a degree of general applicability.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 13
·19738-19752
·
IF 0
Tian Kun,Pan Jie,Liu Yun,Wang Ping,Zhong Ming,Dong Yuanhua,Wang Meng
Heterogeneous Fenton oxidation using traditional catalysts with H 2 O 2 for the degradation of 1,4-dioxane (1,4-DX) still presents challenge. In this study, we explored the potential of Fe-ZSM-5 zeolites (Fe-zeolite) with three Si/Al ratios (25, 100, 300) as heterogeneous Fenton catalysts for the removal of 1,4-DX from aqueous solution. Fe 2 O 3 or ZSM-5 alone provided ineffective in degrading 1,4-DX when combined with H 2 O 2 . However, the efficient removal of 1,4-DX using H 2 O 2 was observed when Fe 2 O 3 was loaded on ZSM-5. Notably, the Brønsted acid sites of Fe-zeolite played a crucial role during the degradation of 1,4-DX. Fe-zeolites, in combination with H 2 O 2 , effectively removed 1,4-DX via a combination of adsorption and oxidation. Initially, Fe-zeolites demonstrated excellent affinity for 1,4-DX, achieving adsorption equilibrium rapidly in about 10 min, followed by effective catalytic oxidative degradation. Among the Fe-ZSM-5 catalysts, Fe-ZSM-5 (25) exhibited the highest catalytic activity and degraded 1,4-DX the fastest. We identified hydroxyl radicals (·OH) and singlet oxygen ( 1 O 2 ) as the primary reactive oxygen species (ROS) responsible for 1,4-DX degradation, with superoxide anions (HO 2 · /O 2 · − ) mainly converting into 1 O 2 and ·OH. The degradation primarily occurred at the Fe-zeolite interface, with the degradation rate constants proportional to the amount of Brønsted acid sites on the Fe-zeolite. Fe-zeolites were effective over a wide working pH range, with alkaline pH conditions favoring 1,4-DX degradation. Overall, our study provides valuable insights into the selection of suitable catalysts for effective removal of 1,4-DX using a heterogeneous Fenton technology.
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FUEL
·
2024
·
IF 6.7
Xueqing Wu,Chuntao Cao,Ruwei Yao,Tingjun Fu,Zhong Li
A core-shell structured S-1@ZSM-5 was prepared by a seed-induced method, and its physicochemical properties and catalytic performance in methanol aromatization were studied based on the growth control of ZSM-5 shell. Results revealed that the ZSM-5 shell can be well coated on the surface of the silicalite-1 (S-1) seed under a low temperature crystallization, which induced the formation of worm-like morphology with a controllable shell thickness of 30 nm. Importantly, the weak acid sites dominated by Lewis acid sites can be constructed based on the control of growth degree. For methanol aromatization, the weak acid sites not only ensured the aromatization reaction, but also inhibited the isomerization and alkylation of para-xylene. The selectivity of aromatics and para-xylene in xylene reached 25.96% and 47.53%, respectively. The thin shell promoted the rapid removal of the coke precursor, which gave the catalyst a relatively long lifetime. Interestingly, on such a shell with weak acid sites, the limited sites can be easily covered by coke deposition along with the reaction progress, and thus the para-xylene selectivity can be gradually increased to 73.81%. Additionally, protective desilication was implemented for core-shell catalysts, and the aromatic selectivity was decreased while the catalytic lifetime and para-xylene selectivity remained unchanged. This reflected the inherent catalytic stability for methanol aromatization due to the thin shell and weak acidity. This work provides a new preparation strategy for ZSM-5 with high para-xylene selectivity and catalytic lifetime based on the direct synthesis of suitable acid sites and the diffusion channels.
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Frontiers of Chemical Science and Engineering
·
2017
·Vol. 11
·期 4
·564-574
·
IF 1.712
Song Baodong,Li Yongqiang,Cao Gang,Sun Zhenhai,Han Xu
In the transformation of methanol to gasoline (MTG), the selectivity to gasoline and the aromatic content in the produced gasoline are important factors. The catalytic activities of steam-treated and non-steam-treated nano-scale H-ZSM-5 (NHZ5) catalysts impregnated with Ag(I), Zn(II) or P(V) have been investigated in a continuous flow fixed bed reactor. The NH 3 -TPD results showed that after impregnation, the Ag/NHZ5, Zn/NHZ5 and P/NHZ5 catalysts contained comparatively more strong, medium-strong and weak acid sites, respectively. Treatment with steam decreased the number of acid sites in all the catalysts, but the pore volumes in the catalysts were larger which improved carbon deposition resistance resulting in prolonged lifetimes. After 6 h of MTG reaction, the selectivity to gasoline for the steam-treated catalysts,
\(Ag_{H_2 O} /NHZ5\) , \(Zn_{H_2 O} /NHZ5\) and \(P_{H_2 O} /NHZ5\) were 70.5, 68.4 and 68.7 wt-%, respectively, whereas their respective aromatic contents in the produced gasoline were 61.9, 55.4 and 39.0 wt-%. Thus \(P_{H_2 O} /NHZ5\) is the most promising catalyst for MTG applications which can meet the China IV gasoline standard that the amount of aromatics in gasoline should be less than 48 wt-%.
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JOURNAL OF BIOTECHNOLOGY
·
2024
·
IF 4.1
Bowen Shen,Lin Zhang,Yu Zhou,Feifei Song,Shengping You,Rongxin Su,Wei Qi
5-Hydroxytryptophan (5-HTP), a precursor of the neurotransmitter serotonin in mammals, has demonstrated efficacy in treating various diseases such as depression, fibromyalgia and obesity. However, conventional biosynthesis methods of 5-HTP are limited by low yield and high reagent and process costs. In this study, the strain C1T7-S337A/F318Y with optimized promoter distribution was obtained, and the 5-HTP yield was 60.30 % higher than that of the initial strain. An efficient fermentation process for 5-HTP synthesis was developed using strain C1T7-S337A/F318Y with whey powder as a substrate for cell growth and inducer production. Shake flask fermentation experiments yielded 1.302 g/L 5-HTP from 2.0 g/L L-tryptophan (L-Trp), surpassing the whole-cell biocatalysis by 42.86 %. Scale-up to a 5 L fermenter further increased the yield to 1.649 g/L. This fermentation strategy substantially slashed reagent cost by 95.39 %, providing a more economically viable and environmentally sustainable route for industrial biosynthesis of 5-HTP. Moreover, it contributes to the broader utilization of whey powder in various industries.
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高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2022
·Vol. 25
·期 1
·402-409
·
IF 3.945
Ran Wang,Fengjin Zhang,Xinghang Pan,Xuming Zheng,Jiadan Xue,Yong Du,Binbin Xie
8-Hydroxy-5-nitroquinoline (NO2-QN-OH) is an antimicrobial, anti-inflammatory and anticancer agent, and has been approved for use in the treatment of diseases. Its photosensitivity, however, cannot be overlooked. The photochemistry of 8-hydroxy-5-nitroquinoline in acetonitrile is investigated using transient absorption and time-resolved resonance Raman spectroscopies. By identifying the short-lived intermediates during the photoreaction, it is clear that the Tn state NO2-QN-OH is generated in 0.8 ps via an ultrafast ISC, followed by the IC in 8.5 ps to produce the T1 state. In neat acetonitrile, the T1 state NO2-QN-OH undergoes intramolecular proton transfer and tautomerizes to form T1 state NO2-QNH-O. To our knowledge, this is the first time that the intramolecular excited state proton transfer of hydroxyl-quinolines in an aprotic polar solvent is observed.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Mingyang Liu,Zhongwen Ouyang,Xiaochen Liu,Jiaojiao Cao,Tongtong Xiao,Zhengcai Xia,Zhenxing Wang
Isolated large-spin Heisenberg antiferromagnetic uniform chain is quite rare. Here, we have successfully synthesized an ideal one-dimensional (1D) S = 5/2 linear-chain antiferromagnet [C2NH8]3[Fe(SO4)3], which crystallizes in a trigonal lattice with the space group R3c. A broad maximum at Tmax = 18 K is observed in the magnetic susceptibility curve. Notably, no long-range magnetic ordering is observed down to 2 K even if the material has a large Curie–Weiss temperature of θCW = −25.5 K. High-field magnetization at 2 K shows a linear increase until saturation at 30 T, and a high-field electron spin resonance (ESR) reveals the absence of a zero-field spin gap. The intrachain interaction J and interchain interaction J′ are determined. Quite a small ratio of J′/J < 2.5 × 10–3 suggests that [C2NH8]3[Fe(SO4)3] behaves as an ideal 1D uniform linear-chain antiferromagnet, in which the magnetic ordering is prevented by the extremely small interchain interaction and quantum fluctuation even for a classical spin of S = 5/2.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 16
·24398-24411
·
IF 0
Xie Yuxuan,Peng Xiao,Song Xin,Ning Ping,Sun Xin,Ma Yixing,Wang Chi,Li Kai
CH 3 SH is a potential hazard to both chemical production and human health, so controlling its emissions is an urgent priority. In this work, a series of transition metal-loaded H-ZSM-5 adsorbents (Si/Al = 25) (Cu, Fe, Co, Ni, Mn, and Zn) were synthesized through the wet impregnation method and tested for CH 3 SH physicochemical adsorption at 60 °C. It was shown that the Cu-modified H-ZSM-5 adsorbent was much more active for CH 3 SH removal due to its abundant strong acid sites than other transition metal-modified H-ZSM-5 adsorbents. The detailed physicochemical properties of various modified H-ZSM-5 adsorbents were characterized by SEM, XRD, N 2 physisorption, XPS, H 2 -TPR, and NH 3 -TPD. The effects of metal loading mass ratio, calcination temperature, and acid or alkali modification on the performance of the adsorbent were also investigated, and finally 20% Cu/ZSM-5 was found to have the best adsorption capacity after calcined at 350 °C. Additionally, the Cu/ZSM-5 adsorbent modified by sodium bicarbonate could expose more active components, which improved the adsorbent’s stability. However, the consumption and reduction of the active component Cu 2+ and the accumulation of sulfate during the adsorption process are the main reasons for the deactivation of the adsorbent. In addition, the simultaneous purging of N 2 + O 2 can effectively restore the adsorption capacity of the deactivated adsorbent and can be used as a potential strategy to regenerate the adsorbent. Graphical
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Rihong Luo,Yuhan Kang,He Ma,Zhenqiang Zhang,Christian Hölscher,Li Hao,Zijuan Zhang
To date, no therapeutic drugs available on the market can effectively reverse the progression of Alzheimer's disease (AD). Although Glucagon-like peptide-1 (GLP-1) receptor agonists (RAs) and Cholecystokinin (CCK) RAs have shown some promise in AD research, little is known about the neuroprotective effects of a novel dual CCK/GLP-1 RA in AD. This study sought to examine the effects of the novel dual CCK/GLP-1 RA on cognitive performance in an AD mouse model and to explore the associated mechanisms. Our findings indicate that dual CCK/GLP-1 RA improved cognitive deficits, reduced amyloid-beta (Aβ) accumulation, and alleviated mitochondrial damage in 5 × FAD mice by inducing mitophagy. In an in vitro model of AD cells induced by Aβ, CCK/GLP-1 RA could exert neuroprotective effects by regulating PINK1/Parkin-mediated mitophagy. These data reveal for the first time that the new CCK/GLP-1 RA modulates mitophagy via PINK1/Parkin pathway and enhances cognitive function in the 5 × FAD animal model. Moreover, the performance of the CCK/GLP-1 RA in certain indicators was superior to that of GLP-1 analogue liraglutide, suggesting that it may represent a more promising therapeutic option for AD.
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高影响因子
Food Bioscience
·
2025
·
IF 5.9
Shuai Wang,Jiayang Li,Cong Xie,Wanhui Chen,Huajie Feng,Wenying He
Our previous study found that 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole (BPT) exhibited very low toxicity and higher biology activity. In the present study, BPT was used to investigate its mechanism against Colletotrichum gloeosporioides in mango by chemical proteomics and chemicobiological methods. The experimental results of some phenotypic traits and cytotoxicity indicated that there are some changes in permeability of the cell membranes and BPT has fungistatic activity with lower cytotoxicity. Next, a biotin probe named BPTN with a higher activity (EC 50 value of 11.08 mg/L) than that of BPT(EC 50 value of 14.13 mg/L) was designed and synthesized by the chemical proteomics approach. The major differentially expressed proteins between BPT or BPTN and Colletotrichum gloeosporioides were identified and analyzed by Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) and bioinformatics. The results indicated that there are 25 differentially expressed proteins in Colletotrichum gloeosporioides under BPT intervention, and 29 target proteins in Colletotrichum gloeosporioides captured by BPTN. For BPTN-anthracnose system, the protein No. 8 belonged to cytochrome P450 proteins (CYP) family can be taken as a target protein of BPTN, which confirmed by bioinformatics analysis. Further, a classic fungal target protein of triazoles, sterol 14α-demethylase (CYP51) was used to verify the interaction in the presence of BPT by various spectroscopic and molecular docking methods. The results showed that BPT exhibited strong interactions with CYP51 and it was predominantly driven by hydrophobic forces and hydrogen bonds. The present study has significant theoretical and practical implications for the development of novel 1,2,3-triazole antifungal agents.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-19
·
IF 10.2
Qiu Lei,Shen Renbin,Wei Lei,Xu Shujuan,Xia Wei,Hou Yan,Cui Jinxin,Qu Rong,Luo Jiale,Cao Jian,Yang Jie,Sun Jing,Ma Ronglin,Yu Qiang
Patients with inflammatory bowel disease (IBD) always suffer from severe abdominal pain and appear to be at high risk for colorectal cancer. Recently, the co-delivery of targeted drugs and gut microbiota has developed into an attractive strategy. A new strategy using gut microbiota fermentation to overcome the interspace diffuse resistance from the mucus layer to control drug release in inflammatory bowel sites (IBS sites) has not yet been available. Here, we designed an alginate hydrogel microsphere encapsulating bifidobacterium (Bac) and drug-modified nanoscale dietary fibers (NDFs). The hydrogel microsphere is responsible for protecting drugs from acidic and multi-enzymatic environments and delivering drugs to the colorectum. Subsequently, the fermentation of Bac by digesting NDFs and proteins as carbon and nitrogen sources can promote drug release and play a probiotic role in the gut microbiota. In vitro evidence indicated that small-sized NDF (NDF-1) could significantly promote short-chain fatty acid (SCFA) expression. Notably, NDF-1 hydrogel microspheres showed a boost release of 5-ASA in the IBS sites, resulting in the amelioration of gut inflammation and remodeling of gut microbiota in chronic colitis mice. This study developed a controlled release system based on microbial fermentation for the treatment of IBD.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Xuemin Zhong,Peng Yuan,Yanfu Wei,Dong Liu,Dusan Losic,Mengyuan Li
The aerobic oxidation of 5-hydroxymethylfurfural (HMF), a key platform compound derived from biomass, to 2,5-furandicarboxylic acid (FDCA) is a highly important reaction in the production of green and sustainable chemicals. Here, we developed a highly efficient and stable halloysite-supported Pt–Au alloy catalyst for the selective oxidation of HMF to FDCA. The catalyst was synthesized through the organosilane functionalization of halloysite nanotubes, followed by the in situ formation and dispersion of Pt–Au alloy nanoparticles on the internal and external surfaces of nanotubes. The composition, morphology, and structure of the prepared catalyst were characterized. The catalyst with the optimal composition of Pt/Au molar ratio of 1/4 and metal loading of 1.5 wt % exhibited outstanding catalytic activity for the oxidation of HMF to FDCA using O2 as an oxidant with 100% conversion of HMF and 99% selectivity of FDCA. This excellent catalytic performance is mainly attributed to the high dispersion and alloying effects of bimetallic nanoparticles, which promoted the activation of reactants or intermediates and further improved FDCA selectivity. Furthermore, the halloysite-supported Pt/Au bimetallic catalyst showed high stability and reusability. This study provides a promising strategy by combining clay mineral halloysite and bimetallic alloys for developing efficient catalysts with high FDCA selectivity and stability for the oxidation of HMF to FDCA.
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