Nature Communications
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2024
·Vol. 15
·期 1
·1-15
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IF 14.7
Zhu Wenjun,Wei Ting,Xu Yuchun,Jin Qiutong,Chao Yu,Lu Jiaqi,Xu Jun,Zhu Jiafei,Yan Xiaoying,Chen Muchao,Chen Qian,Liu Zhuang
Transdermal drug delivery has been regarded as an alternative to oral delivery and subcutaneous injection. However, needleless transdermal delivery of biomacromolecules remains a challenge. Herein, a transdermal delivery platform based on biocompatible fluorocarbon modified chitosan (FCS) is developed to achieve highly efficient non-invasive delivery of biomacromolecules including antibodies and antigens. The formed nanocomplexes exhibits effective transdermal penetration ability via both intercellular and transappendageal routes. Non-invasive transdermal delivery of immune checkpoint blockade antibodies induces stronger immune responses for melanoma in female mice and reduces systemic toxicity compared to intravenous injection. Moreover, transdermal delivery of a SARS-CoV-2 vaccine in female mice results in comparable humoral immunity as well as improved cellular immunity and immune memory compared to that achieved with subcutaneous vaccine injection. Additionally, FCS-based protein delivery systems demonstrate transdermal ability for rabbit and porcine skins. Thus, FCS-based transdermal delivery systems may provide a compelling opportunity to overcome the skin barrier for efficient transdermal delivery of bio-therapeutics.
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Chemical and Biological Technologies in Agriculture
·
2024
·Vol. 11
·期 1
·1-13
·
IF 5.2
Qu Yunhe,Zhang Pingping,Zhao Boya,Xu Jing,Shi Dongfang
The total polysaccharides extracted from Laetiporus sulphureus fruiting bodies by ultrasound-assisted complex enzyme method were separated by freeze–thaw combined with fehling reagent to prepare purified polysaccharide (LSPS1). The results of monosaccharide composition and molecular weight distribution demonstrated that LSPS1 contained galactose (51.83%), mannose (26.89%), fucose (16.13%) and glucose (5.15%), and the molecular weight was around 17.3 kDa. Methylation analysis indicated that the backbone of LSPS1 consisted of 1,6-Gal p residues, branched at O-2 of Gal p with t-Man p , t-Fuc p and t-Glc p residues as side chains. In addition, LSPS1 was used for the synthesis of SeNPs based on the redox system of sodium selenite and ascorbic acid. The L. sulphureus polysaccharide selenium nanoparticles (LSPS1–SeNPs) were characterized by UV, FT-IR, ICP-MS, EDX, DLS and SEM, inferring that LSPS1–SeNPs were stabilized with spherical and granular surface structure as well as a mean particle size of 96.72 ± 1.12 nm. Both of LSPS1 and LSPS1–SeNPs possessed strong antioxidant and hypoglycemic activities while the effects of LSPS1–SeNPs were superior to LSPS1. The findings provided valuable structural information for L. sulphureus polysaccharide, and will promote the application of SeNPs by L. sulphureus polysaccharide in potential antioxidant agent and hypoglycemic candidate. Graphical
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CATALYSIS LETTERS
·
2024
·
IF 2.3
Feng Guo,Wang Xu,Wang Yajie,Sun Hongxin,Li Guohua
In PMS catalytic oxidation studies, we design and prepare a Mn 3 O 4 /CuO (CMO-2) composite catalyst using metal–organic frameworks (MOFs) as precursors. Compared with Mn 3 O 4 , its activated per-oxy-monosulfate activation catalyzed the degradation of pollutants with nearly six times higher activity efficiency. The experimental findings demonstrate that CMO-2 derived from metal–organic frameworks offer the advantages of wide adaptability to solution pH, along with improved versatility, stability, and recyclability. Further experiments involving radical scavenging, electrochemistry, and electron spin resonance (EPR) techniques confirm that the CMO-2 derived from MOF structures exhibit more significant interfacial engineering effects. This leads to an expansion of active interfaces, enhancing the accessibility and dispersion of active sites. Consequently, it significantly improves the efficiency of electron-transfer reactions and promotes a wide range of reactive oxygen species (ROSs), including SO 4 •− •OH and 1 O 2 . Graphical Abstract Based on the synergistic effect of copper-manganese bimetallic, the copper-manganese composite catalyst (CMO-2) prepared by doping 0.5 g of Cu-MOF showed the best catalytic activity in activating PMS, and the resulting ROS S could efficiently degrade organic pollutants.
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Journal of Translational Medicine
·
2024
·Vol. 22
·期 1
·1-12
·
IF 6.1
Tan Yue-Rong,Shen Si-Yang,Li Xin-Yi,Yi Peng-Fei,Fu Ben-Dong,Peng Lu-Yuan
Ulcerative colitis (UC) is an idiopathic, chronic inflammatory condition of the colon, characterized by repeated attacks, a lack of effective treatment options, and significant physical and mental health complications for patients. The endoplasmic reticulum (ER) is a vital intracellular organelle in maintaining cellular homeostasis. Endoplasmic reticulum stress (ERS) is induced when the body is exposed to adverse external stimuli. Numerous studies have shown that ERS-induced apoptosis plays a vital role in the pathogenesis of UC. Mogroside V (MV), an active ingredient of Monk fruit, has demonstrated excellent anti-inflammatory and antioxidant effects. In this study, we investigated the therapeutic effects of MV on dextran sulfate sodium (DSS)-induced UC and its potential mechanisms based on ERS. The results showed that MV exerted a protective effect against DSS-induced UC in mice as reflected by reduced DAI scores, increased colon length, reduced histological scores of the colon, and levels of pro-inflammatory cytokines, as well as decreased intestinal permeability. In addition, the expression of ERS pathway including BIP, PERK, eIF2α, ATF4, CHOP, as well as the apoptosis-related protein including Caspase-12, Bcl-2 and Bax, was found to be elevated in UC. However, MV treatment significantly inhibited the UC and reversed the expression of inflammation signaling pathway including ERS and ERS-induced apoptosis. Additionally, the addition of tunicamycin (Tm), an ERS activator, significantly weakened the therapeutic effect of MV on UC in mice. These findings suggest that MV may be a therapeutic agent for the treatment of DSS-induced UC by inhibiting the activation of the ERS-apoptosis pathway, and may provide a novel avenue for the treatment of UC.
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MICROCHIMICA ACTA
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2024
·Vol. 191
·期 11
·1-14
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IF 5.3
Ren Shufang,Liu Xiaohang,Liu Yahui,Zhao Junpeng,Zhang Yuan,Zheng Zhixiang
A novel voltammetric sensor designed for dopamine (DA) detection is presented utilizing a combination of zeolitic imidazolate framework (ZIF-67) derived cobalt and nitrogen-doped carbon on reduced graphene oxide (Co–N-C/rGO). ZIF-67 cubic crystals were synthesized in situ and deposited onto the graphene oxide (GO) surface through room-temperature reactions. High-temperature calcination resulted in partially collapsed cubic and spherical carbon, while simultaneously reducing GO to rGO. A molecular imprinting resorcinol polymer (MIP) membrane was also in situ applied to the Co–N-C/rGO/glassy carbon electrode (GCE) via electropolymerization. Analyses using cyclic voltammetry, electrochemical impedance, and pulse voltammetry reveal that the modified MIP/Co–N-C/rGO/GCE electrodes show improved electroconductivity and notable electrochemical reactivity towards dopamine. After optimizing detection parameters, the sensor demonstrates a wide linear detection range of 0.01—0.5 and 0.5—100 μmol/L, with a limit of detection (LOD) of 3.33 nmol/L (S/N = 3). Additionally, the sensor displays strong robustness, including excellent selectivity, significant resistance to interference, and long-term stability. It also shows satisfactory recovery in detecting spiked real samples. Graphical
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JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 27
·12347-12360
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IF 3.5
Ding Xiuping,Tao Jianghui,Huang Liangzhu,Wang Yabin,Wang Yanni
Dendritic mesoporous silica nanospheres (DMSNs) possess excellent specific surface areas, pore volumes, and extremely accessible internal spaces. DMSNs have experienced high-speed development in the aspects of synthesis techniques, functionalization strategies, and application fields. It is proved that DMSNs own inherent structural superiorities as catalysts, adsorbents, or reaction platforms. Naturally, researchers are enlightened to conceive and attempt to synthesize dendritic mesoporous titania nanospheres (DMTNs) by replacing Si in DMSNs with Ti, in view of the superior activity and catalytic performance from TiO 2 . Nevertheless, the hydrolysis and condensation rates of the titanium precursors are too fast to develop ideal dendritic textures. To get the goal in a roundabout way, hybrid dendritic mesoporous silica&titania nanospheres (DMSTNs) come into sight. In this work, a series of DMSTNs have been synthesized by the one-pot co-condensation method. For the first time, their morphologies and architectures have been controlled by adjusting the ratio of titanium to silica, stirring speed, reaction temperature, co-solvent, etc. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) have been utilized to directly reveal their differences. The basic physicochemical properties of DMSTNs with fine topological structures have been compared, covering Fourier Transform Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), N 2 adsorption–desorption isotherms, Raman spectrum, X-ray photoelectron spectroscopy (XPS), ultraviolet–visible diffuse reflectance spectroscopy (UV-Vis-DRS), photoluminescence (PL) spectra, etc. Most importantly, these typical DMSTNs can photo-catalytically produce more hydrogen (2.4 ~ 3.6 times) within 1% Pt than that of bare DMSNs under simulated sunlight, owing to Ti in their skeletons. Graphical Abstract
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Science China-Chemistry
·
2025
·
IF 9.7
Wu Min,Nan Mingjun,Liu Shumin,Zhong Caihe,Qiao Lin,Zhang Huamin,Ma Xiangkun
With the increasing demand for energy storage technology, iron-chromium flow batteries (ICFBs) have been widely concerned because of their price advantage. However, the low electrochemical activity of Cr 3+ /Cr 2+ redox couples and the side hydrogen evolution reaction limit the industrial application of ICFBs. A multi-dimensional Bi/carbon composite electrocatalyst (Bi@C) for ICFB is designed and prepared to improve the electrochemical activity of Cr 3+ /Cr 2+ redox couples. Benefiting by using the Bimetal organic framework (Bi-MOF) with solid matrix as precursors, Bi nanospheres are highly dispersed on the Bi@C electrocatalyst that effectively enhances the electrochemical activity. The special morphology of Bi@C electrocatalyst helps the transfer of electrons and ions, significantly reducing the polarization of battery. Herein, the 3D porous carbon frames accelerate mass transfer, and the 2D carbon nanobelts and carbon layer coating on 0D Bi nanospheres improve the conductivity of Bi nanospheres. Therefore, the ICFB with multi-dimensional Bi@C electrocatalyst exhibits coulombic efficiency of 98.10% and energy efficiency of 79.14% at 140 mA cm −2 , which is higher than ICFBs with commercial graphite carbon electrocatalyst and with heat treatment carbon felt. This work provides a simple and economical method to fabricate a high-performance multidimensional Bi@C electrocatalyst for Cr 3+ /Cr 2+ redox couples, boosting the development of ICFBs.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
·
IF 3.8
Zhang Yahui,Wang Xinyi,Jia Changbo,Dong Yongping
The majority of previously reported cathodic electrochemiluminescence (ECL) systems often required very negative potential to be carried out, which has greatly limited their applications in the sensing field. Screening high-performance cathodic ECL systems with low triggering potential is a promising way to broaden their applications. In this work, rhenium disulfide nanosheets (ReS 2 NS) have been revealed as an efficient co-promoter to realize low-triggering-potential cathodic luminol ECL. One strong cathodic ECL signal appeared at a potential of −0.3 V and one anodic ECL peak was obtained at −0.15 V under the reverse potential scan, which were caused by electrogenerated reactive oxygen species (ROS) from hydrogen peroxide. The generation of strong luminol ECL at low potential was the result of the electrocatalytic effect of ReS 2 NS on the reduction of H 2 O 2 . The scavenging effect of uric acid (UA) on the ROS could significantly inhibit the cathodic ECL. As a result, an ECL sensor was proposed, which showed outstanding performance for the detection of UA in the range of 10 nM to 0.1 mM with a low detection limit of 1.53 nM. Moreover, the ECL sensor was successfully applied in the sensitive detection of UA in real samples. This work provides a new avenue to establish a low-potential cathodic ECL system, which will sufficiently expand the potential application of cathodic ECL in the sensing field.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-12
·
IF 14.7
Guo Kexin,Yang Xuehan,Zhou Chao,Li Chuang
Environmentally adaptive hydrogels that are capable of reconfiguration in response to external stimuli have shown great potential toward bioinspired actuation and soft robotics. Previous efforts have focused mainly on either the sophisticated design of heterogeneously structured hydrogels or the complex manipulation of external stimuli, and achieving self-regulated reversal shape deformation in homogenous hydrogels under a constant stimulus has been challenging. Here, we report the molecular design of structurally homogenous hydrogels containing simultaneously two spiropyrans that exhibit self-regulated transient deformation reversal when subjected to constant illumination. The deformation reversal mechanism originates from the molecular sequential descending-ascending charge variation of two coexisting spiropyrans upon irradiation, resulting in a macroscale volumetric contraction-expansion of the hydrogels. Hydrogel film actuators were developed to display complex temporary bidirectional shape transformations and self-regulated reversal rolling under constant illumination. Our work represents an innovative strategy for programming complex shape transformations of homogeneous hydrogels using a single constant stimulus.
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Lipids in Health and Disease
·
2024
·Vol. 23
·期 1
·1-15
·
IF 3.9
Guo XiaoYu,Zhou Jianglu,Yu Hong,Cao Han,Li Xia,Hu Qing,Yu YunQiu
Background Long-chain fatty acids (LCFAs) are involved in regulating multiple physiological processes as signalling molecules. Gas chromatography–mass spectrometry (GC–MS) is widely used to quantify LCFAs. However, current quantitative methods for LCFAs using GC–MS have demonstrated complicated issues. Psoriasis is a chronic inflammatory skin disease, and its pathogenesis may be related to the overproduction of interleukin-17A (IL-17A). Clinical efficacy of anti-IL-17A monoclonal antibody (mAb) treatment in psoriasis patients has been demonstrated. Recent studies suggest that LCFAs play varying roles in the pathogenesis of psoriasis. However, more comprehensive research is needed to illuminate the mechanism of LCFAs in psoriasis. Methods The established in situ derivatization method for analysing LCFAs with a GC–MS platform was utilized to conduct serum lipidomics analysis of healthy volunteers and psoriasis patients receiving pretherapy and posttreatment with of anti-IL-17A mAb. Imiquimod (IMQ)-treated wild type (WT) and T-cell receptor delta chain knock-out ( Tcrd −/− ) mice were used to investigate the correlation between IL-17A and abnormal changes in LCFAs in psoriasis patients. Results A rapid and sensitive in situ extraction derivatization method for quantifying LCFAs using GC–MS was established. Serum lipidomic results showed that psoriasis patients had higher levels of saturated fatty acids (SFAs) and ω-6 polyunsaturated fatty acids (PUFAs) but lower levels of monounsaturated fatty acids (MUFAs) and ω-3 PUFAs than healthy individuals, indicating impaired serum LCFA metabolism. Anti-IL-17A mAb treatment affected most of these LCFA changes. Analysis of LCFAs in IMQ-treated mice showed that LCFAs increased in the serum of WT mice, while there were no significant changes in the Tcrd −/− mice. SFAs increased in IMQ-treated WT mice, while MUFAs showed the opposite trend, and PUFAs did not change significantly. Conclusions This study presented a dependable method for quantifying LCFAs that enhanced sensitivity and reduced analysis time. The lipidomic analysis results showed that anti-IL-17A mAb not only ameliorated skin lesions in psoriasis patients but also affected abnormal LCFAs metabolism. Furthermore, the study indicated a potential correlation between IL-17A and abnormal LCFA metabolism in psoriasis patients, which was supported by the alterations in serum LCFAs observed in IMQ-treated WT and Tcrd −/− mice.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Gong Xu,Liu Shan,Xia Bozhang,Wan Yichen,Zhang Shuyi,Zhang Baoyan,Wang Zehao,Chen Junge,Xiao Fei,Liang Xing-Jie,Yang Yun
Genetically engineered commensal bacteria are promising living drugs, however, their therapeutic molecules are frequently confined to their colonization sites. Herein, we report an oral protein delivery technology utilizing an engineered bacterial type zero secretion system (T0SS) via outer membrane vesicles (OMVs). We find that OMVs produced in situ by Escherichia coli Nissle 1917 (EcN) can penetrate the intact gut epithelial barrier to enter the circulation and that epithelial transcytosis involves pinocytosis and dynamin-dependent pathways. EcN is engineered to endogenously load various enzymes into OMVs, and the secreted enzyme-loaded OMVs are able to stably catalyze diverse detoxification reactions against digestive fluid and even enter the circulation. Using hyperuricemic mice and uricase delivery as a demonstration, we demonstrate that the therapeutic efficacy of our engineered EcN with a modified T0SS outperforms that with a direct protein secretion apparatus. The enzyme-loaded OMVs also effectively detoxify human serum samples, highlighting the potential for the clinical treatment of metabolic disorders.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-15
·
IF 12.6
Wang Xingyi,Fang Hanyi,Hu Wenzhu,Feng Yuan,Zhou Zhangyongxue,Hu Mengyan,Jiang Dawei,Zhang Yongxue,Lan Xiaoli
Limited treatment response and inadequate monitoring methods stand firmly before successful immunotherapy. Recruiting and activating immune cells in the hypoxic tumor microenvironment is the key to reversing immune suppression and improving immunotherapy efficacy. In this study, biomimetic oxygen-delivering nanoparticles (CmPF) are engineered for homologous targeting and hypoxia alleviation within the tumor environment. CmPF targets the tumor microenvironment and delivers oxygen to reduce hypoxia, thereby promoting immune cell activity at the tumor site. In addition, granzyme B-targeted positron emission tomography (PET) imaging is employed to monitor immune cell activity changes in response to immunotherapy efficacy in vivo. The combination of CmPF with carboplatin and PD-1 inhibitors significantly suppresses tumor growth by 2.4-fold, exhibiting the potential of CmPF to enhance the efficacy of immunotherapy. Immunohistochemistry further confirms increased expression of key immune markers, highlighting the reprogramming of the tumor microenvironment. This study demonstrates that hypoxia alleviation enhances tumor immunotherapy efficacy and introduces a non-invasive PET imaging method for dynamic, real-time assessment of therapeutic response.
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