JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-22
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IF 12.6
Shang Xiaofei,Shao Wenjie,Dong Wuqi,Ren Wenxia,Du Wei,Li Chuang,Tian Yuxiang,Fang Weijun,Liu Tao,Xu Qinghua,Zhang Zhongping,Zhao Tingting
The main challenge of photosensitizer in image-guided photodynamic therapy (PDT) is the balance between the high singlet oxygen yields and strong fluorescence performance, while simultaneously targeting certain types of cancer to achieve optimal effect in both imaging and therapy. Herein, a novel donor-acceptor system, engineered with silica coated quantum dots as the donor and a typical porphyrin (T790) as the acceptor, has been further integrated with hierarchical targeting strategy, which demonstrated capability of targeting both hepatic tumors and mitochondria. The prepared nanoparticles were found to exhibit high singlet oxygen generation of 0.86, bright red fluorescence, and a large Stokes shift of 285 nm. In vitro assays revealed that they accumulated within the mitochondria of malignant hepatocytes to induce apoptosis due to their high singlet oxygen generation and disrupt the mitochondrial membrane potential, thereby leveling proteins associated with apoptosis. In vivo studies with xenograft tumor model confirmed their excelllent fluorescence imaging capability and remarkable anti-tumor effects. Transcriptomic analysis further revealed the potential molecular mechanisms of donor-acceptor system in the PDT process. This work provides insights for elucidating the transcriptional regulation of image-guided photodynamic therapy and paves the way for the development of more efficient and precise treatment methods.Graphical
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Haiyan Yao,Yuan Fan,Emine Sumeyra Turali Emre,Jiaolong Wang,Xuewen Wang,Junchao Wei
Bacterial infections and inflammation caused by persistent infections are the primary reasons for delayed healing of infected wounds. Chirality, a prevalent characteristic in nature, plays a crucial role in chemical recognition and interactions between materials and biosystems. Herein, chiral ZnO NPs was prepared and the effects of charity on antibacterial and immune regulation were investigated. D-ZnO NPs showed better antibacterial performance against both S.aureus and E.coli compared with L-ZnO NPs, and the D-ZnO NPs (250 μg/mL) antibacterial rates against of S.aureus and E.coli were 72.2 ± 6.3 % and 98.7 ± 0.22 %, respectively. D-ZnO NPs demonstrated better interaction with bacteria and destroyed bacterial cell walls, leading to the protein leakage and more reactive oxygen species (ROS) production. Furthermore, D-ZnO NPs could suppress over-reacted inflammation and promote the polarization of macrophages towards M2 phenotype. In vivo results showed that D-ZnO NPs effectively promoted the healing of infected wounds, achieving a wound healing rate of 100 % on day 10. This work provides novel insights into the development of chiral and enantioselective nanomaterials, as well as promotes the application of chiral nanomaterials in the biomedical fields.
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BIOORGANIC & MEDICINAL CHEMISTRY
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2025
·
IF 3
Shu-Mei Pan,Wen-Cai Liu,Chun-Yu Xing,Xin-Yue Pu,Qian-Qian Wang,Xuan Zhao,Qi-Chen Zhang,Dan-Dan Wang,Ya-di Zhu
Uridine-5′-diphosphate-glucuronosyltransferase 1A1 (UGT1A1) is a key enzyme in the regulation of bilirubin metabolism and detoxification of a variety of internal and external substances, and its insufficient function can lead to hyperbilirubinemia and affect the metabolic clearance of drugs, food additives, and environmental toxicants. However, there is a lack of safe and effective UGT1A1 inducers in the clinic. This study presents Corylin, an efficient UGT1A1 inducer identified from a series of natural flavonoids and isoflavonoids. Notably, Corylin demonstrated potent induction of intracellular UGT1A1. Furthermore, nuclear receptor reporter gene assays revealed that Corylin dose-dependently activated PPARs and AhR nuclear receptors, with the most robust activation observed in the PPARβ/δ subtype. Animal experiments indicated that Corylin exhibited favorable safety profiles and significant hepatoprotective effects in mice with APAP-induced liver injury, accompanied by marked UGT1A1 expression. Collectively, this study suggests that Corylin is a potential UGT1A1 inducer and a promising candidate for the intervention of liver-related disorders.
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ACS Applied Nano Materials
·
2025
·
IF 5.5
Jun Zhao,Yangfeng Du,Wanqin Cai,Pei Zhang,Xiling Song,Wenxuan Ye,Yunping Peng,Jianfu Zhao,Siming Yu
Nowadays, bacterial infection-associated diseases pose significant threats to human public health. Developing alternative strategies for the highly efficient treatment of bacterial infections is in urgent demand. It is well-known that hydroxyl radical (•OH) exhibits strong antibacterial activity and is widely used for chemodynamic therapy (CDT), while hydrogen sulfide (H2S) displays the ability to promote wound healing for gas therapy (GT). In the present work, a multifunctional nanozyme with pH and GSH dual-responsive sequentially releasing •OH and H2S properties was rationally designed for high-efficiency wound infection treatment via bimodal CDT and GT therapy. Herein, dendritic mesoporous organic silica (DMOS) nanoparticles were prepared and used as the carrier for in situ copper peroxide (CP) nanodots growth, obtaining nanozyme of DMOS@CP. In the acidic condition, CP decorated on DMOS was first decomposed by generating •OH via the Fenton-like reaction, which was able to effectively inhibit bacterial growth, as well as eradicate bacterial biofilms, by disrupting the bacterial cell membrane, increasing intracellular ROS generation, and damaging bacterial DNA. Subsequently, DMOS can be further dissociated by GSH to release a substantial amount of H2S to promote bacterial wound healing. The mechanism study revealed that H2S was capable of first reversing the inflammatory microenvironment of the wound by reprogramming M2-type macrophage polarization, followed by upregulating expressions of hypoxia-inducible factor-1α, vascular endothelial growth factor, and CD31 to promote cell migration and angiogenesis.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Jiacheng Dong,Yu Zhang,Zhongyu Yang,Yanhua Rao,Yueyue Feng,Liying Huang,Shiwei Xu,Guofeng Wang,Nannan Liu
Endocrine-disrupting chemicals, particularly estradiol (E2), pose significant risks to environmental and human health due to their persistence and bioactivity. In this study, we developed a highly sensitive and label-free electrochemical sensor for E2 detection, based on a glass micropipette (GMP) filled with polydopamine nanotubes (PDA-NTs) and coupled with a small molecule-induced DNA strand dissociation strategy. PDA-NTs self-assemble into a densely packed structure at the tip of the GMP, providing abundant binding sites for E2 aptamer (Apt) immobilization and achieving the first signal amplification, which yields a detection limit of 1.29 pM. To further enhance sensitivity, a partially complementary DNA (cDNA) strand was hybridized with the aptamer to form an Apt-cDNA duplex. Upon E2 binding, the Apt in the Apt-cDNA duplex undergoes a conformational change that releases the sterically large cDNA, resulting in a significant increase in ionic current and enabling the second signal amplification. This second amplification improves the detection limit by 112-fold, reaching as low as 11.5 fM. The sensor can detect E2 in the detection range of 10 fM–10 nM and demonstrates high selectivity in complex mixed solutions such as fetal bovine serum (FBS) and environmental water. Beyond highly sensitive detection of E2, this dual-signal amplification also allows the detection of other small molecules through aptamer sequence replacement.
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FOOD HYDROCOLLOIDS
·
2026
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IF 13.8
Xiangrui Ren,Liyuan Ma,Diandian Wang,Liping Zhao,Haiyu Luo,Yanyun Hu,Wenhao Li
This study proposes a novel method for efficiently modifying native chitosan (NCS) by employing electron beam irradiation (EBI) in conjunction with citric acid (CA)-associated modification, thereby overcoming the limitations of NCS application, including poor solubility and restricted functionality. The results confirmed that EBI effectively reduced the apparent viscosity-average molecular weight of NCS and exposed more accessible sites, thereby facilitating subsequent CA-associated modification. Comprehensive analyses (FT-IR, XRD, DSC, and SEM) confirmed that the synergy of physical activation and chemical modification disrupted the hydrogen-bond network of NCS at the molecular level and reduced its crystallinity. These structural changes generated a porous morphology and significantly improved pH-dependent solubility stability, thereby preventing precipitation in neutral to alkaline environments. Antioxidant and antibacterial properties are also enhanced, which may be attributed to exposed active sites, CA-associated groups, and improved solution clarity. Ultimately, NCS derivatives with improved pH-dependent solubility stability, strong antioxidant activity, and excellent antibacterial performance were obtained. PCA suggested an association between apparent viscosity-average molecular weight reduction and functional improvement. These findings provide a feasible strategy for improving the applicability of chitosan in food-related systems.
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MICROCHEMICAL JOURNAL
·
2026
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IF 5.2
Jinping Tang,Fuqiang Qian,Yuhai Wang,Chunxu Wang,Yuan Chai,Dewen Kong,Jin Wang
By integrating the photonic nanojet effect of polystyrene microsphere (PS), the charge transfer capability of MXene nanosheets, and the electromagnetic field enhancement induced by Ag nanoparticles, the PS@MXene@Ag composite substrate exhibited outstanding SERS sensitivity. • The SERS performance of the PS@MXene@Ag substrate was evaluated using 2-mercaptopyridine as a probe molecule, achieving a detection limit as low as 1.24 × 10 −12 M, with the SERS performance factor (SPF) up to 5.0 × 10(Gueguen et al., 2000 [7] ) . In addition, the SERS substrate demonstrated satisfactory results in signal uniformity, batch-to-batch reproducibility, and long-term stability. • PS@MXene@Ag composite successfully detected thiram and methyl blue on practical sample surfaces (orange and fish), achieving satisfactory recovery rates. Surface-enhanced Raman scattering (SERS) spectroscopy has emerged as a powerful analytical technique for the sensitive detection of hazardous molecules, owing to its ultranarrow spectral linewidths and highly specific molecular fingerprinting capability. Herein, we designed a reliable SERS substrate consisting of polystyrene microspheres (PS), Ti 3 C 2 T x MXene nanosheets, and Ag nanoparticles (NPs) for the sensitive detection of various organic pollutants. By integrating the photonic nanojet effect of PS, the charge transfer capability of MXene nanosheets, and the electromagnetic field enhancement induced by Ag NPs, the PS@MXene@Ag composite substrate exhibited outstanding SERS sensitivity, along with excellent reproducibility and signal uniformity. As a result, the limit of detection (LOD) for 2-mercaptopyridine (2-Mpy) was as low as 1.24 × 10 −12 M, with the SERS performance factor (SPF) of up to 5.0 × 10 7 . Moreover, the SERS substrate maintained good stability after storage for four weeks. In addition, the PS@MXene@Ag composite was successfully applied to the detection of other organic pollutants, including thiram and methylene blue (MB). The calibration results revealed a good linear relationship between the logarithm of concentration and the corresponding SERS intensity, with correlation coefficients (R 2 ) of 0.99 and 0.98, respectively. The LODs for thiram and MB were calculated to be 1.96 × 10 −10 M and 2.33 × 10 −10 M, respectively, and satisfactory recovery rates were obtained in practical sample analyses (orange peel and fish skin). These results demonstrate that the fabricated PS@MXene@Ag SERS substrate has promising potential for the detection of organic molecular pollutants. Download: Download high-res image (129KB) Download: Download full-size image PS@MXene@Ag as a SERS-active substrate for the detection of organic pollutants.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Yahong Li,Junjin Zhu,Xin Zhang,Yuanyuan Li,Shu Zhang,Linxin Yang,Ruyi Li,Qianbing Wan,Xibo Pei,Junyu Chen,Jian Wang
It has been confirmed that substantial vascularization is an effective strategy to heal large-scale bone defects in the field of bone tissue engineering. The local application of deferoxamine (DFO) is among the most common and effective methods for promoting the formation of blood vessels, although its short half-life in plasma, rapid clearance, and poor biocompatibility limit its therapeutic suitability. Herein, zeolitic imidazolate framework-8 (ZIF-8) was selected as a vehicle to extend the half-life of DFO. In the present study, a nano DFO-loaded ZIF-8 (DFO@ZIF-8) drug delivery system was established to promote angiogenesis–osteogenesis coupling. The nanoparticles were characterized, and their drug loading efficiency was examined to confirm the successful synthesis of nano DFO@ZIF-8. Additionally, due to the sustained release of DFO and Zn2+, DFO@ZIF-8 NPs were able to promote angiogenesis in human umbilical vein endothelial cells (HUVECs) culture and osteogenesis in bone marrow stem cells (BMSCs) in vitro. Furthermore, the DFO@ZIF-8 NPs promoted vascularization by enhancing the expression of type H vessels and a vascular network. The DFO@ZIF-8 NPs promoted bone regeneration in vivo by increasing the expression of OCN and BMP-2. RNA sequencing analysis revealed that the PI3K-AKT-MMP-2/9 and HIF-1α pathways were upregulated by DFO@ZIF-8 NPs in HUVECs, ultimately leading to the formation of new blood vessels. In addition, the mechanism by which DFO@ZIF-8 NPs promoted bone regeneration was potentially related to the synergistic effect of angiogenesis–osteogenesis coupling and Zn2+-mediation of the MAPK pathway. Taken together, DFO@ZIF-8 NPs, which were demonstrated to have low cytotoxicity and excellent coupling of angiogenesis and osteogenesis, represent a promising strategy for the reconstruction of critical-sized bone defects.
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TISSUE & CELL
·
2023
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IF 2.6
Ying Wang,Yongjun Li,Dongxia Liu
Background This study aimed to investigate role of erythropoietin in atherosclerosis and explore whether underlying mechanism is associated with PI3K/AKT/mTOR pathway. Methods High-fat-diet-induced atherosclerosis model was established in apolipoprotein E knockout mice (C57BL/6 genetic background). Mice were randomly divided into the control group and the EPO group. Hematoxylin-eosin was performed for the determination of atherosclerotic lesions. The expression levels of related proteins were detected by western blot analysis . Results Erythropoietin significantly enhanced the incidence of hemorrhage in atherosclerotic plaques compared with the control group. The proteins’ expression signaling pathways (including PI3K , AKT, and mTOR) and angiogenesis-related proteins (VEGF, COX-2, and HIF-1α) were proved to be up-regulated by erythropoietin. Additionally, erythropoietin significantly enhanced the incidence of hemorrhage in the atherosclerotic plaques compared with the control group. The vitro experiments were conducted in macrophages at 21% O 2 or 1% O 2 . The data showed that expression of p -PI3K, p -AKT, p -mTOR, VEGF, COX-2, and HIF-1α related proteins increased in 1% O 2 group than 21% O 2 group. Moreover, compared with control group, protein expression including p -PI3K, p -AKT, p -mTOR, VEGF, COX-2, and HIF-1α was markedly increased in EPO group, decreased in inhibitors group, and similar results were observed in EPO+ inhibitors group. Conclusion The present study demonstrated that erythropoietin might promote angiogenesis in atherosclerotic vulnerable by activating PI3K/AKT/mTOR signaling pathway in atherosclerotic, providing a novel therapeutic target for atherosclerotic targeted therapy.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2023
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IF 5
Ying Sun,Yongxiang Zhang,Xiaxia Liu,Yan Tingting,Liyan Shen,Dan Ye,Xia Kong,Yonghui Su,Qingping Tian
Transfersomes is one of the most effective nanotechnology-based formulation for transdermal delivery of minoxidil (MXD), however the potential use was limited by its low drug content ( DC ) and entrapment efficiency ( EE ). In this study, efforts have been made to improve DC and EE of MXD in transfersomes for effective topical treatment of alopecia . By dissolving both MXD and edge activator (Tween 80) in aqueous phase than either or both of them co-dissolving in organic solvent with lipids (phospholipid and cholesterol with mass ratio of 5:1), the DC and EE of MXD transfersomes prepared by thin film hydration method were significantly increased. The optimized transfersomes (MT) and its gel (MT gel ) with mass ratio of drug, Tween 80 and lipids 3:1:18 have a spherical morphology without aggregation, and a particle size of not more than 100 nm. The differential scanning calorimetry measurements revealed that the drug in MT was in the non-crystalline state. MT and MT gel exhibited better in vitro transdermal penetration ability and hair of C57BL/6 mice growth-promoting capacity than commercial formulations. MT gel has non-irritation and is better than MT in skin adhesion, stability, potential for skin storage and hair growth promotion ability, which could endow it to be a promising strategy for topical treatment of alopecia.
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International Journal of Nanomedicine
·
2023
·
IF 8
Yao Chen,Shuya Liu,Yin Liao,Hanshan Yang,Zhuo Chen,Yuru Hu,Shaozhi Fu,Jingbo Wu
Background Considering the strong attenuation of photons and the potential to increase the deposition of radiation, high-atomic number nanomaterials are often used as radiosensitizers in cancer radiotherapy, of which gold nanoparticles (GNPs) are widely used.Materials and Methods We prepared albumin-modified GNPs (Alb-GNPs) and observed their radiosensitizing effects and biotoxicity in human non-small-cell lung carcinoma tumor-bearing mice models.Results The prepared nanoparticles (Alb-GNPs) demonstrated excellent colloidal stability and biocompatibility at the mean size of 205.06 ± 1.03 nm. Furthermore, clone formation experiments revealed that Alb-GNPs exerted excellent radiosensitization, with a sensitization enhancement ratio (SER) of 1.432, which is higher than X-ray alone. Our in vitro and in vivo data suggested that Alb-GNPs enabled favorable accumulation in tumors, and the combination of Alb-GNPs and radiotherapy exhibited a relatively greater radiosensitizing effect and anti-tumor activity. In addition, no toxicity or abnormal irritating response resulted from the application of Alb-GNPs.Conclusion Alb-GNPs can be used as an effective radiosensitizer to improve the efficacy of radiotherapy with minimal damage to healthy tissues.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Rui Ma,Jinrong Bai,Yuting Huang,Zhiqiu Wang,Yongju Xu,Yan Huang,Kai Zhong,Yina Huang,Hong Gao,Qian Bu
Peanut (Arachis hypogaea) peptides have various functional activities and a high utilization value. This study aims to isolate and characterize antioxidant peptides from peanut protein hydrolysates and further evaluate their neuroprotection against oxidative damage to PC12 cells induced by 6-hydroxydopamine (6-OHDA). After the peanut protein was hydrolyzed with pepsin and purified using ultrafiltration and gel chromatography, six peptides were identified and sequenced by high-performance liquid chromatography (HPLC) and liquid chromatography–tandem mass spectrometry (LC–MS/MS). Out of these six peptides, Pro-Gly-Cys-Pro-Ser-Thr (PGCPST) exhibited a desirable antioxidant capacity, as determined using the 1,1-diphenyl-2-picrylhydrazyl, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and hydroxyl radical scavenging assays. Moreover, our results indicated that the peptide PGCPST effectively increased the cell viability and reduced the cell apoptosis in 6-OHDA-induced PC12. RNA sequencing further showed that the neuroprotective effect of the peptide PGCPST was mediated via sphingolipid metabolism-related pathways. With further research efforts, the peptide PGCPST was expected to develop into a new neuroprotective agent.
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