INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Xingzi Lan,Yurong Liu,Yaqi Wang,Feng Tian,Xiaomin Miao,Han Wang,Yadong Tang
Preparing wound dressing with dual-delivery of antioxidant and antibacterial agents is highly desirable in clinical wound treatment. Herein, a series of coaxial nanofiber membranes loaded with antioxidant tea polyphenols (TP) in the core and antibacterial ε-poly (L-lysine) (ε-PL) in the shell layer were successfully fabricated by coaxial electrospinning. The physicochemical characterizations by transmission electron microscopy , inverted fluorescence microscopy and fourier transform infrared spectroscopy confirmed the formation of core-shell structure. The results of in vitro drug release indicated that ε-PL exhibited a fast release profile while TP released in a sustained manner, which is favorable to the achievement of quick bacteria inhibition in the initial phase as well as long-term antioxidant activity during wound healing. The antioxidant activity of coaxial nanofibers was found to be increased with the increment of TP content and incubation time . The antibacterial assays against Escherichia coli and Staphylococcus aureus demonstrated that the incorporation of ε-PL in the coaxial nanofibers led to strong antibacterial activity . Additionally, all the coaxial nanofibers possessed good cytocompatibility. Therefore, the prepared coaxial nanofibers simultaneously incorporated with ε-PL and TP are promising as potential wound dressing materials.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 20
·14774-14788
·
IF 2.993
Hu Yang,Liu Shuifeng,Li Xin,Yuan Teng,Zou Xiuju,He Yinyan,Dong Xianming,Zhou Wuyi,Yang Zhuohong
Biocompatible porous scaffolds with tunable microstructures and drug delivery ability have aroused increasing attention in the application of the biomedical fields, especially in tissue engineering. In this study, we have facilely fabricated the poly( l -lactic acid)-modified halloysite nanotubes (m-HNTs)/poly(ε-caprolactone) (PCL) porous scaffolds by direct solvent evaporation of m-HNTs stabilized water in oil Pickering emulsion templates, which contain PCL in the oil phase. The obtained scaffolds have possessed the porous microstructures, which can be easily tailored by varying the preparation conditions of emulsion templates including m-HNTs concentrations and the volume ratios of water to oil. Furthermore, the antibacterial drug enrofloxacin (ENR) has been loaded into the scaffolds, and the in vitro release studies show the potential of m-HNTs/PCL porous scaffolds as drug carriers. And the antimicrobial test results have proved that the ENR-loaded porous scaffolds exhibit obvious and long-term antibacterial activity against Escherichia coli . In addition, mouse bone mesenchymal stem cells (mBMSCs) are cultured on the m-HNTs/PCL porous scaffolds, and the results of cell counting kit-8 assay and confocal laser scanning microscope observation show that the m-HNTs/PCL porous scaffolds are cytocompatible, because mBMSCs can attach, develop and proliferate well on the porous scaffolds. All the results indicate that the m-HNTs/PCL porous scaffolds hold great potential applications in tissue engineering as scaffolds and/or drug carriers.
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高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 10
·2738-2749
·
IF 5.831
Weijun Zhang,Kunxi Zhang,Guifei Li,Shifeng Yan,Lei Cui,Jingbo Yin
Shape memory scaffolds are minimally invasive cell carriers that are promising biomaterials for tissue regeneration. Since cell fate is critical for successful regeneration, the influence of mechanostructural stimuli induced by shape memory on cell fate is worthy of investigation. In this study, we developed a poly(L-glutamic acid)-based (PLGA-based) shape memory porous scaffold by cross-linking PLGA with poly(ε-caprolactone)-diols (PCL-diols) and by using the particle leaching method. After regulating the cross-linking density and molecular weight of the PCL-diols, the scaffolds exhibited excellent shape memory properties around physiological temperatures. The interconnected porous structure not only enabled the scaffold to be deformed to 20% of its original size but also supported tissue invasion. In vivo results demonstrated that the PLGA-based scaffold degraded within 6 months. Cell fate studies indicated that large dimensional deformation of the porous structure during the shape memory process induced significant death, detachment and reorganization of stem cells but had negligible effects on stemness and proliferation. These results indicate that the PLGA-based shape memory porous scaffold is a potential cell carrier for tissue regeneration, and they are also meaningful to investigate the effects of mechanostructural stimuli on stem cell fate in porous structures.
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高影响因子
Small
·
2021
·Vol. 17
·期 32
·2101495
·
IF 13.281
Jingwu Zhu,Jiang Tian,Chao Yang,Junpeng Chen,Lihuang Wu,Mengni Fan,Xiaojun Cai
The development of alternative strategies for the efficient treatment of subcutaneous abscesses that do not require the massive use of antibiotics and surgical intervention is urgently needed. Herein, a novel synergistic antibacterial strategy based on photodynamic (PDT) and NO gas therapy is reported, in which, a PDT-driven NO controllable generation system (Ce6@Arg-ADP) is developed with l -Arg-rich amphiphilic dendritic peptide (Arg-ADP) as a carrier. This carrier not only displays superior bacterial association and biofilm penetration performance, but also acts as a versatile NO donor. Following efficient penetration into the interior of the biofilms, Ce6@Arg-ADP can rapidly produce massive NO via utilizing the H 2 O 2 generated during PDT to oxidize Arg-ADP to NO and l -citrulline, without affecting singlet oxygen ( 1 O 2 ) production. The combination of 1 O 2 and the reactive by-products of NO offers notable synergistic antibacterial and biofilm eradication effects. Importantly, following efficient elimination of all bacteria from the abscess site, Arg-ADP can further generate trace quantities of NO to facilitate the angiogenesis and epithelialization of the wound tissues, thereby notably promotes wound healing. Together, this study clearly suggests that Arg-ADP is a versatile NO donor, and the combination of PDT and NO represents a promising strategy for the efficient treatment of subcutaneous abscesses.
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高影响因子
BIOMATERIALS
·
2013
·
IF 7.604
Jingbin Huang,He Zhang,Yuan Yu,Yan Chen,Dong Wang,Guoqing Zhang,Guichen Zhou,Junjie Liu,Zhiguo Sun,Duxin Sun,Ying Lu,Yanqiang Zhong
To develop biodegradable docetaxel-loaded self-assembled nanoparticles of poly ( d , l -lactide-co-glycolide)/hyaluronic acid block copolymers were successfully synthesized. These copolymers could form nanoparticles with small size (<200 nm), an acceptable CMC (∼7.9 mg/L), typical core/shell structure and superior stability in one week. DTX-loaded PLGA 502H -b-HA 5.6k nanoparticles (DTX/SANPs) showed a biphasic release pattern within 120 h, and exhibited enhanced cytotoxicity toward CD44-overexpressing MDA-MB-231 cells. Cellular uptake study indicated that PLGA 502H -b-HA 5.6k nanoparticles (SANPs) were taken up in MDA-MB-231 cells by CD44-mediated endocytosis . Pharmacokinetics study revealed DTX/SANPs could prolong the circulation of DTX in the blood. In vivo studies demonstrated that SANPs exhibited enhanced tumor targeting and antitumor activity with lower systemic toxicity. In conclusion, DTX/SANPs have great potential for targeted chemotherapy for CD44-overexpressing breast cancer.
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高影响因子
Materials Today Communications
·
2019
·
IF 1.859
Henan Wang,Zeyu Li,Shuting Lu,Chunyu Li,Wancheng Zhao,Yuliang Zhao,Shuling Yu,Ting Wang,Tiedong Sun
Biocompatibility and targeted therapy are the demand by modern chemotherapy drugs. Meanwhile, the utilizations of biopolymer or environmentally friendly polymer are also the key issues for sustainable development. We have constructed a new drug delivery system with targeted and controlled release capabilities. It has been confirmed that an environmentally friendly polymer (CMC- g -PLA) obtained by grafting polylactic acid on a carboxymethyl cellulose biopolymer has an amphiphilic property and is easy to form micelles , thereby being useful as a hydrophobic drug carrier. In order to have the function of targeted therapy, anti-EpCAM antibody was anchored on the polymer chain of carboxymethyl cellulose by amidation reaction. Doxorubicin and HepG2 cells were used as the model anticancer drug and tumor targeting cells, respectively. The nano scale core-shell structure, critical micelle concentration , drug loading and encapsulation efficiency were studied by TEM, pyrene fluorescent probe method and ultraviolet standard curve method, respectively. Furthermore, the pH induced release function experiments, cytotoxicity experiments and in vivo tumor treatments experiments on mice. The results show that the drug carrier with antibody prepared by us can effectively deliver hydrophobic drugs to specific tumor sites and increase the effect of tumor treatment. This versatile design provides a new solution for future cancer treatments.
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高影响因子
Journal of Biobased Materials and Bioenergy
·
2021
·Vol. 15
·期 4
·553-558
·
IF 0.708
Chen Zhifeng,Guo Haifeng,Sui Changling,Gao Zhixi,Wang Tianhong,Luo Yong,Qin Cheng,Guan Xiaoxi
Drought and salt are main environmental factors that affect the growth, development, productivity and distribution of plants of plants. Alfalfa has a strong ability of early and salt resistance. In this work, the varieties Xinjiang Daye was used as material, the effects of drought (simulated
with PEG, polyethylene glycol-6000) and salt stress (with NaCl solution) on the antioxidant capacity of alfalfa seedlings and stress resistance genes was studied, to select alfalfa varieties with strong resistance and study its functional principle of resistance related genes. The results
showed that with the increase of drought stress, the contents of H2O2, O2−, MDA increased by 323, 247 and 235 (15% PEG treatment). The activities of SOD, CAT and APX increased by 18.01, 15.56 and 587% (15% PEG treatment), respectively. The expression
of drought resistance genes increased significantly. With the increase in NaCl stress, the activities of SOD, cat, pod and APX increased by 132.14, 315.60, 102.78 and 27.61%, respectively. The expression of two genes related to salt stress increased significantly. In conclusion, alfalfa leaves
have good survival ability under high stress, and the activities of antioxidant enzymes and the expression of related genes have adaptive changes under drought and salt stress.
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2023
·
IF 11.4
Lei Chen,Daijie Chen,Shunyi Zhou,Jieying Lin,Yun Liu,Xiongfei Huang,Qingqi Lin,Jean Louis Morel,Zhuobiao Ni,Shizhong Wang,Rongliang Qiu
Hexafluoropropylene oxide (HFPO) homologues, which are important alternatives to perfluorooctanoic acid, have been frequently identified in crops. Although exposure to HFPO homologues via crops may pose non-negligible threats to humans, their impact on crops is still unknown. In this study, the accumulation, transport, and distribution mechanisms of three HFPO homologues in lettuce were investigated at the plant, tissue, and cell levels. More specifically, HFPO trimer acid and HFPO tetramer acid were primarily fixed in roots and hardly transported to shoots (TF, 0.06–0.63). Conversely, HFPO dimer acid (HFPO-DA) tended to accumulate in lettuce shoots 2–264 times more than the other two homologues, thus resulting in higher estimated daily intake values. Furthermore, the dissolved organic matter derived from root exudate enhanced HFPO-DA uptake by increasing its desorption fractions in the rhizosphere. The transmembrane uptake of HFPO homologues was controlled by means of a transporter-mediated active process involving anion channels, with the uptake of HFPO-DA being additionally facilitated by aquaporins. The higher accumulation of HFPO-DA in shoots was attributed to the larger proportions of HFPO-DA in the soluble fraction (55–74%) and its higher abundance in both vascular tissues and xylem sap. Our findings expand the understanding of the fate of HFPO homologues in soil–crop systems and reveal the underlying mechanisms of the potential exposure risk to HFPO-DA.
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高影响因子
Russian Journal of Physical Chemistry A
·
2019
·Vol. 93
·期 4
·696-702
·
IF 0.581
Huanxin Li,Zhang Haihan,Dou Yonghui,Xu Li,Liu Guoji
Abstract The solubility of (1R,2R)-(+)-1,2-diaminocyclohexane L-tartrate (DHT) in methanol + water, ethanol + water, and 2-propanol + water mixed solvents were measured using a synthetic method with alcohols mass fractions varying from 0.1 to 0.5 over 278.15 to 318.15 K under atmospheric pressure ( P = 0.1 MPa). It was proved that the solubility of DHT in the three binary solvents increased with increasing temperature, while decreased with the mass fraction of alcohols rising. The experimental solubility data were correlated by the modified Apelblat equation and Sun model. The modified Apelblat model showed better agreement with the experimental data than the other model. Furthermore, the thermodynamic parameters including \(\Delta G_{{{\text{sol}}}}^{^\circ }\) , \(\Delta H_{{{\text{sol}}}}^{^\circ }\) , and \(\Delta S_{{{\text{sol}}}}^{^\circ }\) for DHT in the dissolution process were obtained from Van’t Hoff plots.
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高影响因子
ULTRASONICS SONOCHEMISTRY
·
2014
·
IF 3.816
Wang Liju,Keyume Ablajan,Feng Jun
A rapid and efficient ultrasound-promoted one-pot synthesis of spiro[indoline-3,4′-pyrano[2,3-c]pyrazole] derivatives is described here. The reaction of substituted phenylhydrazine and dialkyl acetylenedicarboxylate with substituted isatin and malononitrile is catalyzed by l -proline to give good-to-excellent yields in water/ethanol (v/v, 1:1) at room temperature.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 2
·928
·
IF 5.924
Muhammad Inam,Lu Liu,Jian-Wei Wang,Ka-Xi Yu,Chi-Uyen Phan,Jie Shen,Wen-Hua Zhang,Guping Tang,Xiurong Hu
Puerarin (PUE) is a Chinese traditional medicine known to enhance glucose uptake into the insulin cells to downregulate the blood glucose levels in the treatment of type II diabetes. Nevertheless, the bioavailability of pristine PUE is limited due to its poor solubility and low intestinal permeability. In this work, we demonstrate that the solubility of PUE can be significantly enhanced via its co-crystallization with L-Proline (PRO). Two crystalline phases, namely, the solvate-free form [PUE][PRO] (I) and the solvated form [PUE]2[PRO]∙EtOH∙(H2O)2(II) are isolated. These two phases are characterized by single-crystal X-ray diffraction (SCXRD), powder X-ray diffraction (PXRD), Fourier-transformed infrared (FT-IR) spectra, nuclear magnetic resonance (NMR), and thermogravimetric analysis in association with differential scanning calorimetry (TGA-DSC). The solubility and dissolution rate of bothIandIIin water, gastrointestinal tract at pH 1.2, and phosphate buffer at pH 6.8 indicates a nearly doubled increase as compared to the pristine PUE. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of pristine PUE,IandIIagainst murine colon cancer cell lines CT-26 and human kidney cell lines HEK-293 indicated that neither compound exhibits obvious cytotoxicity after 24 h. This work showcases that the readily available and biocompatible PRO can be a promising adjuvant to enhance the physicochemical properties of PUE toward orally administered drug formulation with improved pharmacokinetics.Keywords:puerarin;L-proline;pharmaceutical co-crystal;crystal structure;solubility and dissolution rate;Chinese traditional medicine;Hirschfeld analysis;hydrogen-bonding
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高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Liang Dong,Hui Chen,Ting Liu,Junming Zhu,Min Yu,Qingmei Yuan
Three disulfide bond-containing peptide amphiphiles (PA1–3) with different lengths of alkyl tails (PA1 for C6, PA2 for C12, and PA3 for C18) were synthesized by ring-opening polymerization of α-amino acid N-carboxyanhydride followed by post-polymerization modification. The peptide segments were composed of 3-mercaptopropionic acid-modified poly(l-cysteine) [P(Cys-SS-CH2CH2COOH)]. We characterized the chemical structure and molecular parameters by 1H NMR, 13C NMR, gel permeation chromatography, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy. It is found that alkyl-P(Cys-SS-CH2CH2COOH) mainly presents a β-sheet conformation at the solid state. However, the PAs present predominant random coils at pH 7.4 in aqueous solutions. The β-sheet conformation increased dramatically when the concentration of the PA exceeded its critical micelle concentration (ca. 0.3 mg/mL for PA3), indicating the formation of self-assembly-induced β-sheet nanostructures. Elongation of the alkyl chain length or a decrease of the pH of the PA solution can promote the formation of the β-sheet conformation. The three PAs can self-assemble into spherical micelles or nanofibrous hydrogels, which can be utilized as nanocarriers for systemic drug delivery or implants for localized drug delivery, respectively. Cisplatin (CDDP) was loaded as a model medicine to examine the drug delivery potential of PA3. We found that the CDDP-loaded PA3 micelles are stable at pH 7.4, have a spherical morphology with a hydrodynamic diameter of ca. 52 nm, and accomplish pH/reduction dual-responsive release of CDDP. In addition, alkyl-P(Cys-SS-CH2CH2COOH) can self-assemble into nanofibrous hydrogels at pH 5.0–6.0 or upon the addition of certain metal ions and show excellent dynamic reversibility. Moreover, the CDDP-loaded PA3 hydrogel exhibits a sustained release profile and a nearly linear release over 48 h. In vitro cytotoxicity of PA3 also indicates its nontoxicity. Thus, our findings suggest that alkyl-P(Cys-SS-CH2CH2COOH) has great potential for both systemic and localized delivery of therapeutics.
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高影响因子
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