JOURNAL OF MOLECULAR LIQUIDS
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2020
·
IF 5.065
Linghua Zhuang,Fei Zhong,Mengyi Qin,Yu Sun,Xiaoyan Tan,Huiwen Zhang,Mengke Kong,Kehui Hu,Guowei Wang
Two imidazolium chloride ionic liquids (ILs) were mixed with urea for chitosan dissolution. Ionic liquids were [Amim]Cl and [Etmim]Cl (1-allyl-3-methyl-imidazolium chloride and 3-methyl-1-(ethylacetyl)imidazolium chloride, respectively). Physicochemical properties of neat ILs and IL-Urea solutions (including density, viscosity, conductivity, β value, π* value, and thermogravimetric analysis), were thoroughly investigated. Dissolution performance of chitosan in IL-Urea solutions was determined. Chitobiose (KET) as model chitosan structure, density functional theory (DFT) calculations were used to discuss the interactions between IL, urea, and chitobiose, and to clarify the different physicochemical properties of ILs, IL-Urea solutions. Also DFT simulation results were used for elucidating the influence of different cationic substituents and urea addition for chitosan dissolution. [Etmim]Cl showed higher density, viscosity, conductivity, β, and π* value than those of [Amim]Cl. These results revealed strong hydrogen bonds among [Etmim]Cl owing to the carboxylic acid ester group than allyl group of [Amim]Cl. Densities, viscosities, and π* values of IL-Urea solutions increased with increasing urea content. Conductivities and β values of IL-Urea solutions decreased with the increase of urea content at same temperature. The thermal stability of [Amim]Cl-Urea mixture increased when urea content increased from 10% to 30%, which was different from [Bmim]Cl-Urea melt. The thermal stability of [Etmim]Cl-Urea mixture was different to that of [Amim]Cl-Urea mixture. Urea was accommodated in ILs, forming hydrogen bonds with both the anion and cation in ILs, which changed the molecular interactions of ILs. The influence of different substituents in imidazolium ILs and urea addition on chitosan dissolution were analyzed using experimental determination and DFT calculations. Cationic substituents of imidazolium ILs play an extraordinary role in hydrogen bonds formation with chitobiose, so to strengthen chitosan dissolution. The information obtained would provide a guide for designing novel IL solvent systems for chitosan dissolution.
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ACS Applied Bio Materials
·
2020
·
IF 0
Ting Wu,Shaobin Yu,Dongzi Lin,Zhimin Wu,Jun Xu,Jinglin Zhang,Zefen Ding,Ying Miao,Tao Liu,Tao Chen,Xiang Cai
With double cross-linking of chitosan (CS) and sodium alginate (SALG), a colon-targeting CS/SALG hydrogel bead was prepared and used for the colon-targeted release of doxorubicin hydrochloride (DOX). The CS/SALG hydrogel bead showed the improved mechanical property to withstand the simulated colon intestinal fluid (SCF), small intestinal fluid (SIF), as well as gastric fluid (SGF). DOX was released slightly in SGF and SIF and rapidly in SCF, showing a controlled DOX release from the CS/SALG hydrogel bead under different physiological conditions. In vitro cytotoxicity and in vivo toxicity assays indicated that the DOX-loaded CS/SALG hydrogel bead displayed obvious inhibition to tumor cells. These results suggested that the CS/SALG hydrogel bead had the potential application as a colon-targeting oral formulation for DOX transport.
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CARBOHYDRATE POLYMERS
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2020
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IF 7.182
Renliang Lyu,Ting Xia,Cheng Liang,Cheng Zhang,Zeqin Li,Lichun Wang,Yi Wang,Ming Wu,Xiaogang Luo,Jiayu Ma,Cunwen Wang,Caili Xu
Three kinds of novel environmentally benign and high-efficiency crude oil demulsifiers were prepared using methoxy polyethylene glycol (MPEG) to modify alkylated carboxymethyl chitosan (ACMC). Structures of the demulsifiers were confirmed using FT-IR and 1 H NMR, and the relationship between surface tension and concentration was tested. Demulsification performance was investigated using the bottle test method with oil-in-water (O/W) emulsions that were prepared in lab conditions. The demulsification efficiency was as high as 79.1 %–84.9 %, and the possible mechanism of the demulsification process is discussed. Results show that MPEG-grafted ACMC (MPEG-ACMC) has a promising application as a demulsifier for dealing with emulsified O/W crude oil.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
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2020
·Vol. 108
·期 9
·1890-1898
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IF 3.525
Lilong Gao,Hui Zhang,Bing Yu,Wenlong Li,Fei Gao,Ke Zhang,Hongbo Zhang,Youqing Shen,Hailin Cong
Skin lesions and injuries can increase the risk of pathogen infections. Developing efficacious wound dressings could effectively prevent bacterial infection and accelerate wound healing. Herein, we developed chitosan composite hydrogels cross-linked by multifunctional diazo resin (DR) as antibacterial dressings for improved wound healing. The composite hydrogels were in situ formed by electrostatic interactions, chelation interactions, and covalent bonds between carboxylated chitosan and DR under ultraviolet assisted without small photosensitizer. The resultant hydrogels (noted as DR-CCH) showed good stability at different DR concentrations in physiological buffers. The antibacterial assays showed the DR-CCH could inhibit and kill Escherichia coli and Staphylococcus aureus . What is more, our hydrogels could accelerate wound healing in vivo. The present study demonstrates this composite DR-CCH with trace zinc has potential for accelerated wound healing.
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CARBOHYDRATE POLYMERS
·
2019
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IF 6.044
Xiao Ke,Mingyan Li,Xiaoqing Wang,Jiawen Liang,Xing Wang,Shaoyun Wu,Ming Long,Chengheng Hu
Acute myocardial infarction (MI) is a common cardiovascular disease with high mortality. In this study, an injectable thermosensitive hydrogel of chitosan (CS)/dextran (DEX)/β–glycerophosphate (β-GP) loaded with umbilical cord mesenchymal stem cells (UCMSCs) was prepared for MI treatment. The good biocompatibility of hydrogels was confirmed by 3T3 cells and HUVECs culture study in vitro . HUVECs encapsulated in the hydrogels showed a cell delivery ability. Furthermore, the results indicated that hydrogel could encapsulate most of UCMSCs and the cells exhibited good viability in CS/ 1.0DEX/β-GP hydrogels. The expression of cardiac markers of cTnI and Cx43 and signaling pathways of p-Akt and p-ERK1/2 were studied, and it showed that UCMSCs differentiate towards myocardium and has a great potential for therapeutic use of cardiac repair. In conclusion, the thermosensitive hydrogel of CS/1.0 DEX/β-GP loaded UCMSCs was a promising candidate as cell delivery vehicle for cardiac repair to reconstitute damaged myocardium.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Qi Wenbo,Xu Lijian,Zhao Shuangdan,Zheng Jiahua,Tian Yanpeng,Qi Xuejun,Huang Xianghua,Zhang Jingkun
Herein we report a facile approach for chitosan-heparin hydrogels with controlled release manner and their applications for intrauterine adhesion. The sol precursor was converted to gel at physiological temperature in 15 min. FTIR, SEM and swelling test were performed to characterize their compositions, morphologies and stability. In vitro releasing profiles was investigated in PBS solutions. Intrauterine injured rat model was established and treated with different methods. The results of H&E staining, Masson trichrome staining, western blots assay, immunohistochemical staining and immunofluorescence staining revealed that endogenous c-kit positive stem cells (HSCs) were recruited to the injury site and promoted the wound recovery. After 7 days' treatment, uterus treated with SDF-1α releasing hydrogel showed no difference with control group on endometrial thickness, glands number and fibrosis level. This work provides a possible method for intrauterine adhesion healing.
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APPLIED CLAY SCIENCE
·
2019
·
IF 3.89
Zhiming Sun,Jie Xu,Gaofeng Wang,Ankang Song,Chunquan Li,Shuilin Zheng
Mycotoxins contamination has been a worldwide long-standing issue, which poses tremendous threats to human health and food security. However, the detoxification of various mycotoxins, especially apolar species, is still a big challenge at present. In this study, carbon nanoparticles derived from biopolymer chitosan (Cts) and rectorite supported Cts carbon nanocomposite ( [email protected] ) were fabricated via hydrothermal treatment as efficient mycotoxin adsorbents for apolar zearalenone (ZER). Microstructural analysis revealed that Cts nanoparticles with smaller sizes uniformly distributed on the surface of rectorite, forming the [email protected] biocomposite . The obtained two adsorbents contained high organic carbon contents and large specific surface areas, which exhibited superior adsorption efficiency towards ZER. Kinetic study indicated that the adsorption of ZER on Cts and [email protected] reached equilibrium within 120 min, and the adsorption process fitted pseudo-second-order kinetic model better. The adsorption isotherms of ZER on both adsorbents matched obviously with linear model, which implied the existence of adsorption/partition model mechanism. The adsorption capacities were found to be positively correlated to the organic carbon contents and specific surface areas, suggesting that organic matter functioned as partition medium for ZER and the enhanced specific surface areas provided sorption sites for ZER. Furthermore, no desorption happened when changed the simulated pH based on the values from gastric fluid to intestinal fluid, confirming the feasibility of resultant Cts and [email protected] biocomposite as mycotoxins adsorbents in vivo.
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JOURNAL OF MATERIALS SCIENCE
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2019
·Vol. 55
·期 5
·2176-2185
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IF 3.442
Ghorbani Marjan,Nezhad-Mokhtari Parinaz,Sohrabi Hessamaddin,Roshangar Leila
In the last years, chitosan (CS) nanofibers as one of the biodegradable biomaterials in nature for tissue engineering and related fields, including wound healing and cell–material interaction, have been widely used. For this purpose, we prepared the reinforced CS/nanocrystals cellulose-graft-poly( N -vinylcaprolactam) (CS/NCC-g-PVCL) nanofibers via the electrospinning technique. Fabricated nanofibers were characterized and studied for their structural, morphological, thermal stability, as well as mechanical and hydrophilic properties. Uniform nanofibers were achieved, and NCC-g-PVCL contents were partially embedded into CS nanofibers, as revealed in SEM analyses. Incorporation of NCC-g-PVCL contents (0.5, 2.5, and 5 wt%) enhanced the average fiber diameter of the obtained nanofibers from 100 nm (neat CS) to ∼ 350 nm [CS/NCC-g-PVCL (5 wt%)] and improved the nanofibers thermal stability. Additionally, among the CS/NCC-g-PVCL nanocomposite fibers, those loaded with 5 wt% NCC-g-PVCL had the best mechanical properties. The water contact angle of nanocomposite nanofibers increased with elevation of the weight content of NCC-g-PVCL. Cell culture results showed that the prepared nanofibers had better cytocompatibility and proliferation than neat CS nanofibers. The results proposed that the developed CS/NCC-g-PVCL nanocomposite nanofibers were promising as new biomaterials to be applied in the area of skin tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Xiangmei Ruan,Puwang Li,Chao Wang,Zuyu He,Yunhao Liu,Chuang Zhou,Liqing Du,Shuhui Song,Ziming Yang
Chitosan (CS) is a natural marine polysaccharide with good biocompatibility and biodegradability . But its poor water solubility and antibacterial activity limit its application in fruits preservation. In this study, based on the advantage of quaternary phosphonium salt (QP) and salicylic acid (SA) with good antibacterial activities and different antibacterial mechanisms, a novel antibacterial coating material was synthesized by grafting QP and SA onto CS. With the grafting of SA and QP onto CS, not only the crystallinity of CS molecules decreased and the water solubility improved, but also the antibacterial activity of CS-QP-SA against Escherichia coli and Staphylococcus aureus , and Colletotrichum gloeosporioides (anthracnose) improved by the synergistic effect of QP and SA. After 20 days storage, the mango fruits treated by CS-QP-SA had a weight loss rate of 12.86 %, the fruit decay incidence was 52.00 ± 1.70 %. Hence, the CS-QP-SA films effectively extending the storage time of mango fruits to a certain extent. The results of this study indicated that CS-QP-SA might be a promising preservative for fruits and vegetables.
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CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Rongyu Zhang,Jingxin Ding,Xiaoyu Lu,Aihua Yao,Deping Wang
Multifunctional drug delivery system that can treat bone infection and concurrently accelerate bone tissue regeneration is highly desirable. In this study, we developed a pH-responsive local drug carrier in which a core/shell microsphere consisting of a borate glass core and a mesoporous hydroxyapatite HA shell (BG-HA) was coated with glutaraldehyde-crosslinked chitosan (CS). At neutral pH, a negligible concentration of vancomycin was released from the [email protected] microspheres. However, in an acidic environment, the pores on the HA shell were uncapped due to the swelling of CS, thus accelerating the drug release. The advantage of the [email protected] was evidenced by comparing with [email protected] that was prepared by fully converting borate glass followed by CS coating. The dissolution of the unconverted borate glass core upon immersing in PBS led to an increase in the local pH. This change of pH regulated the swelling/de-swelling behaviours of CS, thereby realizing more sustained drug release. Furthermore, it revealed that the vancomycin-loaded [email protected] provided highly effective antibacterial activity against S. aureus and E. coli , while the vancomycin-loaded [email protected] did not show inhibitory effect on E. coli . The results indicated that the [email protected] would be a promising multifunctional delivery system that can achieve self-regulated drug release, control the acidic environment of bone infection sites and promote bone tissue regeneration.
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Foods
·
2023
·Vol. 12
·期 15
·2898
·
IF 5.2
Lingyu Han,Ruiyi Zhai,Bing Hu,Jixin Yang,Yaoyao Li,Zhe Xu,Yueyue Meng,Tingting Li
The synthesis of octenyl-succinylated chitosan with different degrees of substitution resulting from chemical modification of chitosan and controlled addition of octenyl succinic acid was investigated. The modified products were characterized using1H NMR, FTIR, and XRD, and the degree of substitution was also determined. The properties of the modified chitosan oligosaccharide in solution were evaluated by surface tension and dye solubilization, finding that the molecules self-assembled when they are above the critical aggregation concentration. The two methods yielded consistent results, showing that the self-assembly was reduced with higher levels of substitution. The antimicrobial activity of the octanyl-succinylated chitosan oligosaccharide (OSA-COS) derivatives againstStaphylococcus aureus,Escherichia coli, andFusarium oxysporum f.sp cucumerinumwas investigated by the Oxford cup method. While the acetylated COS derivatives were not significantly effective against eitherE coliorS. aureus, they showed significant antifungal activity towardF. oxysporumthat was superior to that of COS. The modified product was found to form a stable emulsion when mixed with whey protein isolate. The emulsion formed by the highly substituted derivatives have a certain stability and loading efficiency, which can be used for the encapsulation and delivery of astaxanthin.Keywords:chitosan oligosaccharide;octenyl succinic anhydride;astaxanthin;emulsions properties
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Advanced Healthcare Materials
·
2023
·
IF 10
Ning Yang,Xiuhua Pan,Xiawei Zhou,Zengyi Liu,Jie Yang,Jun Zhang,Zengguang Jia,Qi Shen
Immunogenic cell death (ICD) shows promising therapeutic potential for tumor regression. However, the low sensitivity and immunosuppressive state of current cell death manners seriously impede tumor immunogenicity. Ferroptosis characterized by excessive lipid peroxidation (LPO), has emerged as a potential strategy to induce ICD and activate antitumor immune responses. However, the effectiveness of ferroptosis is limited by antioxidant regulatory networks, including the glutathione peroxidase 4 (GPX4) and ferroptosis suppressor protein 1 (FSP1) pathways, presenting challenges for its induction. Herein, we propose a novel approach using functionalized chitosan-ferrocene-sodium alginate (CFA) crosslinked nanogels, incorporating pravastatin (PRV) and M1 macrophage membrane (MM) (designing as CFA/PRV@MM). Specifically, ferrocene boots intracellular reactive oxygen species (ROS) levels for efficient glutathione (GSH) depletion through Fenton reaction, thus disrupting the GPX4/GSH axis, while PRV intervenes in the mevalonate (MVA) pathway to inhibit the FSP1/CoQ10 antioxidant axis, thereby synergistically causing pronounced ferroptotic damage and promoting ICD. The CFA/PRV@MM nanogels demonstrate superior therapeutic efficacy in a mouse breast model, resulting in effective tumor ablation and immune response with minimal side effects. RNA transcription analysis reveals that nanogels can significantly affect metabolic progress, as well as immune activation. This research provides valuable insights into the design of ferroptosis induction for cancer immunotherapy. This article is protected by copyright. All rights reserved
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