ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Zitong Wei,Xiaoyan Dong,Yan Sun
The intricate pathophysiology of Alzheimer’s disease (AD), characterized by β-amyloid (Aβ) deposition, oxidative stress, and neuroinflammation, presents significant challenges for conventional single-target therapeutic approaches, thereby necessitating the development of innovative multifunctional theranostic strategies. Herein, red fluorescence cerium-based carbon dots (CCP-CD) were designed and synthesized via a one-step solvothermal method by purposely selecting curcumin (Cur) for inhibitory and anti-inflammatory effects, Ce(NO3)3·6H2O for enhancing antioxidant activity, and p-phenylenediamine (p-PD) for modulating fluorescence property as precursors. The as-prepared CCP-CD potently suppressed Aβ fibrillization and strongly eliminated multiple reactive oxygen species (ROS) (·OH, O2·–, DPPH·) at low concentrations (1–10 μg/mL). Moreover, CCP-CD facilitates detection of Aβ plaques through a turn-on red fluorescence mechanism for both in vitro and in vivo imaging and quantification. In vitro assays showed that CCP-CD markedly attenuated Aβ-induced cytotoxicity and increased cell viability from ∼60 to 95%, as well as exhibited excellent anti-inflammatory effects by reducing the levels of TNF-α and IL-6 in BV-2 cells. In vivo experiments revealed that CCP-CD effectively suppressed Aβ plaque formation in the AD nematodes and prolonged the lifespan from 13 to 20 d. The unique structural characteristics of CCP-CD, including its conjugated aromatic framework, reversible Ce3+/Ce4+ redox pair, and abundant O- and N-containing functional groups, facilitate balanced multiple therapeutic and diagnostic functions. The work has thus provided an exceptional nanoagent for detecting Aβ and fighting against Aβ-associated toxicities.
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NEUROPHARMACOLOGY
·
2025
·
IF 4.6
Xiaofan Li,Guang Shi,Wanshu Guo,Kaishu Wen,Yifei Liu,Jiayue Cheng,Meichi Liu,Yuehui Yang,Xinyu Fan
DNA methylation plays a pivotal role in neuroprotection after ischemic stroke. Oxytocin, a neuropeptide renowned for its involvement anti-inflammatory and antioxidant activities, emerges as a promising candidate for the treatment of stroke. Here, we employed a transient middle cerebral artery occlusion (tMCAO) rat model to explore the underlying mechanisms of oxytocin's therapeutic potential. We utilized the Morris Water Maze, novel object recognition, and intruder tests to assess spatial memory, cognitive memory, and social memory abilities in rats, respectively. RNA sequencing and Western blotting were employed to detect alterations in mRNA expression and protein expression levels in the hippocampus. Daily oxytocin administration (0.1 and 1.0 mg/kg, subcutaneous) for seven days significantly reduced infarct volume, restored the expression of 1100 genes, including NQO1 and HO1, and robustly promoted hippocampal neurogenesis in tMCAO rats. These effects translated into enhanced neurological function and improved learning and memory abilities. Remarkably, our findings reveal that oxytocin-induced neurogenesis is intimately linked to the activation of the Wnt3a/β-catenin signaling pathway with Nrf2 as a key downstream target. Furthermore, it is possible that oxytocin, acting through its receptors, inhibited the tMCAO-induced binding of DNMT1 to MeCP2, thereby preventing the increase in DNA methylation at the Wnt3a gene. In summary, our study implies a possible involvement of oxytocin in the activation of the DNMT1-mediated Wnt3a/β-catenin signaling pathway. It may contribute to enhancing hippocampal neurogenesis and ameliorating memory impairments in rats with ischemic stroke. This hints that oxytocin holds great promise as a potential therapeutic agent for promoting post-stroke neurogenesis.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Xiujun Ren,Qiurong Luo,Di Zhou,Kailian Zhang,Die Gao,Qifeng Fu,Jun Liu,Zhining Xia,Lujun Wang
A novel chiral stationary phase (CSP) was prepared through the reaction of surface-initiated atom transfer radical polymerization (ATRP) by the copolymerization of thermoresponsive N -isopropylacrylamide (NIPAM) and β-cyclodextrin (β-CD) on the silica beads for high performance liquid chromatography (HPLC). X-ray photoelectron spectroscopy (XPS), elemental analysis (EA), Fourier transform infrared spectrometry (FT-IR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM) were applied to characterize the surface property of modified silica. Thermoresponsive modulation for the effect on enantioselectivity were investigated with chiral reagents including 1-phenyl-1-propanol, styrene oxide, 2-phenylpropionic acid and commercial chiral drugs comprising ibuprofen and labetalol hydrochloride. The column efficiency was evaluated by chromatographic parameters including retention factor (k), selective factor (α), resolution (R s ), plate number (N) and peak tailing factor (T f ). The results showed that five chiral solutes could be separated on the prepared smart column. And the selectivity of these compounds could be modulated by regulating the column temperature. It was contributed to the thermoresponsive NIPAM assisting β-CD to separate these chiral compounds. These results indicated that the thermoresponsive CSP would be a potential tool for separation of hydrophilic and hydrophobic chiral drugs and this paper provided a novel method for chiral separation in the future.
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BIORESOURCE TECHNOLOGY
·
2012
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IF 4.98
Zheng-Zheng Zou,Hui-Lei Yu,Chun-Xiu Li,Xin-Wen Zhou,Chihiro Hayashi,Jie Sun,Bao-Hong Liu,Tadeyuki Imanaka,Jian-He Xu
A new β-glucosidase (DtGH) representing 40% identity with an apple seed glycosidase (ASG) was cloned from Dictyoglomus thermophilum . DtGH showed extremely high thermostability in aqueous solution, with half-lives of 533, 44, and 5 h measured at 70, 80 and 90 °C, respectively. Therefore it was used for direct glycosylation of n -octanol at 70 °C instead of 50 °C as usually. As a result, the glucose based conversion was increased by 27%, but the time spent to reach equilibrium was decreased from 7 d to 3 d. This enzyme also exhibited excellent stability under the reaction environment, retaining 70–80% of its initial activity after 7 d of incubation at 70 °C in either 1.7 M glucose solution or octanol-aqueous (85:15, v/v) system. It could retain part of synthetic activity even in boiling water. Owing to the strong glucose-tolerance and extremely high thermostability, DtGH should be promising for various glucosides synthesis.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ye-Tao Zhang,Li-Guo Liao,Min Tan,Xu-Wei Qi,Fu-Zhong Zhang,Min-Feng Zeng,Minglong Yuan,Bang-Jing Li,Sheng Zhang
Formaldehyde is widely used in daily life and can be generated endogenously through diverse biological processes. However, formaldehyde may cause severe threat to the health of humanity. In this work, we designed a novel nanoprobe NaP@Pβ-CD through the host-guest self-assembly between poly β-cyclodextrins (Pβ-CD) and naphthalimide (NaP). The NaP is enclosed in the Pβ-CD cavity, forming hydrophilic nanoparticles. Formaldehyde can trigger “on” fluorescence response of NaP fluorophore and the detection can be carried out in aqueous solution. This probe has low detection limit (16.8 nM), a fast response to FA (5 min), high selectivity, good biocompatibility and excellent stability. More importantly, it shows excellent sensing capability totally in water without requiring any organic solvent. Therefore, the NaP@Pβ-CD could be successfully applied to formaldehyde detection in food, living cells, and organisms in vivo .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xiao-hua Chen,Xiao-min Li,Jie Deng,Jin-mei Li,Di-qiu Liu
Fusarium wilt is a significant problem restricting the growth of the lily industry. However, Lilium regale Wilson is a valuable germplasm resource because of its Fusarium Wilt resistance. In this study, six endo -β-1,4-glucanase genes ( Cel1-Cel6 ) in L. regale were identified among the differentially expressed genes reveled by omics data for L. regale infected Fusarium osysporum , which causes Fusarium wilt. Interestingly, the abundance of a few proteins and the expression of endo -β-1,4-glucanase genes, including LrCel1 , decreased in L. regale roots infected with F. oxysporum. Thus, the function of LrCel1 in the incompatible interaction between L. regale and F. oxysporum was analyzed. LrCel1 expression was inhibited by F. oxysporum and salicylic acid (SA). Additionally, the subcellular localization of LrCel1 revealed it was a cell membrane protein. The ectopic expression of LrCel1 increased of susceptibility of tobacco to F. oxysporum because of the associated increase in cellulase activity, which, hindered the production of cellulose, callose, and lignin. Moreover, LrCel1 overexpression inhibited the accumulation of SA and jasmonic acid (JA), resulting in the down-regulated expression of genes associated with SA/JA signaling pathways. Conversely, introducing an RNAi vector targeting LrCel1 into L. regale enhanced the resistance to F. oxysporum , likely because of an increase in the synthesis of cellulose, callose, and lignin as well as a decrease in the expression of JA/SA signaling pathway genes. In addition, LrCel1 promoter activity was suppressed by F. oxysporum and Fusarium solani as well as SA. Furthermore, a positive regulator of Fusarium wilt resistance, LrWRKY11, negatively regulated LrCel1 promoter activity. The study findings have elucidated the intricate regulatory network of WRKY transcription factor and SA/JA signaling pathways to inhibit the cellulase activity of endo -β-1,4-glucanase in L. regale , thereby conferring resistance to Fusarium wilt.
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POLYMER ENGINEERING AND SCIENCE
·
2025
·
IF 3.2
Hui-Hong Luo,Wen-Rong Xu
pH-responsive supramolecular polymeric vesicles were successfully developed through the host–guest complexation of two biocompatible and water-soluble saccharides: sulfobutylether-β-cyclodextrin (SCD) and N -dodecyl chitosan (DCS). Extensive characterization using UV–vis spectroscopy, dynamic light scattering (DLS), zeta potential measurements, and transmission electron microscopy (TEM) confirmed the structural robustness of the vesicles, which exhibited effective self-assembly in acidic conditions and disassembly in alkaline environments. To evaluate the drug-loading capacity and pH-responsive release properties, naproxen sodium (NS), a widely used nonsteroidal anti-inflammatory drug, was encapsulated as a model compound. In vitro release studies simulating gastrointestinal pH conditions (2.0, 6.8, and 8.5) demonstrated minimal drug release of (28.34 ± 1.02)% at low pH and significantly enhanced release of (56.57 ± 1.68)% and (86.96 ± 1.29)% at neutral and slightly alkaline pH, respectively. The system exhibited an initial burst release followed by sustained release, aligning with the therapeutic requirements for rapid onset and prolonged action. These findings highlight the potential of SCD/DCS supramolecular vesicles as a versatile platform for targeted oral drug delivery, particularly for drugs requiring intestinal release. Highlights Supramolecular vesicles self-assembled via sulfobutylether-β-cyclodextrin (SCD) and N -dodecyl chitosan (DCS) host–guest complexation. Vesicles exhibited stability in acidic pH and disassembled in alkaline conditions. Naproxen sodium-loaded vesicles displayed pH-sensitive release properties. Drug release occurred in neutral to alkaline pH, ideal for intestinal delivery.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Siru Guo,Huiting Han,Xinru Gao,Yilin Zhai,Juan Wang,Zhaona Zhang,Xuetong Liu,Ruoyu Yuan,Lina Yang,Xinxing Wang
Bisphenol A (BPA), as an endocrine disruptive compound of public concern, is widely present in natural water and wastewater. The development of BPA detection technology is of great significance. However, the insoluble organic nature of BPA hinders its adsorption and electron transfer at hydrophilic sensing interfaces, limiting the improvement of electrochemical sensing performance. Based on the high affinity and selectivity of β -cyclodextrin ( β -CD) toward BPA, a composite material (CNT@CD-MOF) of β -cyclodextrin metal–organic framework (CD-MOF) embedded with carbon nanotubes (CNTs) was prepared in this article for electrochemical sensing of BPA. Compared with β -CD, the three-dimensional ordered structure of CD-MOF promoted the adsorption and enrichment of BPA. The doping of CNTs led to the growth of smaller sized CD-MOF, improved the material’s charge transfer ability, and enhanced its stability. Under the synergistic effect of CNTs and CD-MOF, the CNT@CD-MOF modified glassy carbon electrode exhibited a catalytic effect on BPA oxidation and significantly improved response signals, achieving detection of BPA in a fairly wide linear range of 1 nM ∼ 500 μM with a low detection limit of 0.5 nM. This study successfully applied functionalized CD-MOF for the electrochemical detection of BPA for the first time, greatly improving the electrochemical sensing performance of BPA and promoting the application of electrochemical sensing technology in BPA detection. In addition, the excellent properties of CNT@CD-MOF make it of great application value in the fields of BPA adsorption and removal.
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DRUG DELIVERY
·
2022
·
IF 6.819
Wanmei Wang,Yan Liu,Pan Pan,Yueqi Huang,Ting Chen,Tianyu Yuan,Yulong Ma,Guang Han,Jiahuan Li,Yiguang Jin,Fei Xie
Smoke bombs are often used in military/fire training, which can produce a large amount of zinc chloride (ZnCl2) smoke. Inhalation of ZnCl2 smoke usually causes acute lung injury (ALI) that would likely develop to acute respiratory distress syndrome (ARDS). However, there is no effective prevention or treatment strategy for the smoke-induced ALI. Resveratrol (RES) is a natural polyphenol with good anti-inflammatory and anti-apoptotic activities, but its low solubility, stability, and bioavailability restrict its clinical application. In this study, an inhalable RES formulation composed of RES-β-cyclodextrin inclusion complexes (RES-β-CD) was prepared for the prevention of ZnCl2 smoke-induced ALI. RES-β-CD powders had a small mass median aerodynamic diameter of 3.61 μm and a high fine particle fraction of 38.84%, suitable for pulmonary inhalation. RES-β-CD exhibited low BEAS-2B cytotoxicity. Pulmonary delivery of RES-β-CD to mice remarkably prevented the smoke-induced ALI with downregulation of TNF-α, IL-1β, STAT3, and GATA3, and upregulation of T-bet and Foxp3. RES-β-CD protected the respiratory function, percutaneous oxygen saturation, physical activity, lung capillary integrity, and lung liquid balance, alleviating inflammation and apoptosis. Pulmonary delivery of the positive drug, budesonide (BUD), also alleviated the smoke-induced ALI by reduction of inflammation and cell apoptosis. RES-β-CD exhibited the regulation of the Th1/Th2 and Treg/Th17 balances, while BUD did not show any effect on immune balances. In conclusion, pulmonary delivery of RES-β-CD is a promising anti-inflammatory and anti-apoptosis strategy for the prevention of ZnCl2 smoke-induced ALI by direct lung drug distribution and regulation of immune balance.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2021
·Vol. 32
·期 12
·1-13
·
IF 3.896
Xu Wanghan,Tan Wei,Li Chan,Wu Keke,Zeng Xinyi,Xiao Liwei
Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment. It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis. In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15). The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET. The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro. This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis. After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization. Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis. To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis. Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.
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BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Zhipeng Xie,Jinlong Lei,Mingfeng Yang,Youji Li,Xinhua Geng,Simin Liu,Jiahai Wang
Amine group-bearing small molecules tend to adsorb onto the nanochannel surfaces, which degrades the efficiency of nanochannel sensors. In this study, we utilized host-guest knowledge to eliminate the influence of excessive small molecules. In combination with single-walled carbon nanotubes (SWNTs), β-cyclodextrin (β-CD) provided an excellent sensing performance for the conical nanochannel coated with polyethyleneimine (PEI) and zirconium ion (Zr 4+ ). By taking detection of melamine as a prototype, the as-prepared nanochannel could selectively detect melamine-elicited double-stranded DNA (dsDNA). Both excessive melamine and single-stranded DNA were removed via the addition of β-CD and SWNTs. The nanochannel sensing platform for the detection of melamine has a detection limit of as low as 4.3 nM with a suitable sensitivity, an excellent reproducibility and stability. Therefore, it is highly promising that a nanochannel sensor based on ion current rectification (ICR) can be used to detect other possible targets.
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Redox Biology
·
2019
·
IF 7.793
Shen-Fei Wang,Xin Liu,Mo-Yu Ding,Shuangcheng Ma,Jing Zhao,Ying Wang,Shaoping Li
Reducing agents are crucial for the management of maladaptive inflammation-induced macrophage death and hematopoietic toxicity of chemotherapy. 2- O - β - d -glucopyranosyl- l -ascorbic acid (AA-2 β G), a unique AA (or vitamin C) derivative identified in Lycium barbarum , exhibited enhanced free radical scavenging activity compared with AA and its synthetic derivative AA-2 α G. AA-2 β G protected hydrogen peroxide-induced cell death in murine macrophage RAW264.7 cells. Treatment with AA-2 β G eliminated oxidative stress and the ratio of cellular glutathione to glutathione disulfide more effectively than AA and AA-2 α G. AA-2 β G also significantly reduced the fluorescent intensity of DCFH-DA triggered by chemotherapeutic agent camptotehcin-11 but not fluorouracil. AA, AA-2 α G, and AA-2 β G significantly decreased Keap-1expression, and increased the expression levels of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1. All compounds triggered the nuclear translocation of Nrf2, while the ability of AA-2 β G to enhance the Nrf2-DNA binding affinity was approximately two fold as those of AA and AA-2 α G. Sodium ascorbate cotransporters (SVCT) inhibitors, sulfinpyrazone, phloretin, and 3- O -methyglucose, potently abrogated the free radical scavenging activities of AA, AA-2 α G, and AA-2 β G. The cellular uptake efficacy of AA-2 α G and AA-2 β G was less than 10% of AA, while the inhibition of SVCT with sulfinpyrazone considerably diminished the uptake efficacy of these compounds. AA-2 α G and AA-2 β G are more stable in the Fenton reagents than AA. In summary, AA-2 β G from L. barbarum with excellent free radical scavenging activity is a promising natural AA derivative for further pharmacological evaluation.
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