JOURNAL OF MATERIALS SCIENCE
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2025
·Vol. 60
·期 40
·18933-18945
·
IF 3.9
Liu Guiqun,Jiao Pengwei,Ma Qisheng,Li Ding,Wu Wenli,Xue Min,Zhang Xiaoli
Conducting polymer-based bioadhesive conductive interface (CP-BCI) are widely used in bioelectronics, strain sensors due to their ease of processing and fabrication, excellent electrical, and biocompatibility. However, CP-BCI is conventionally fabricated using techniques such as cast molding, screen printing, electrochemical patterning, and photolithography. These traditional methodologies often encounter challenges in achieving precise control and the generation of intricate patterns, thus limiting the widespread application and evolution of these materials. Direct ink writing has become an emerging research hotspot for processing CP-BCI due to its high degree of programmability, precision, and process stability. Herein, biointerface inks with enhanced electrical conductivity and high adhesion have been specifically developed for direct ink printing. The development of this novel ink involves the incorporation of PAM into the PVA matrix to promote composite cross-linking, coupled with the integration of PEDOT:PSS conductive nanofibers within the matrix, thereby yielding the PEDOT:PSS-PAM-PVA biointerface ink. The innovative ink showcases exceptional printability, high electrical conductivity (0.24 ± 0.053 S m −1 ), high adhesion (54 ± 2.7 kPa), and a well-balanced mechanical profile, featuring a maximum stress of 51 kPa and an elongation at break of 369%. This research introduces the novel direct writing ink, promoting advancements in the manufacturing of flexible electronics. Graphical abstract
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-23
·
IF 12.6
Xi Zhipeng,Xu Ning,Zhou Xiaobo,Chen Peng,He Yanwei,Wan Renwen,Zhang Longlong,Ding Zhipu,Cai Weiye,Chen Yisheng,Luo Zhiwen,Zhang Wei,Lin Chengshou,Chen Shuo,Zhao Zhijie,Liu Chao,Li Jingchi
Osteochondral defects pose significant challenges in joint health, leading to pain, decreased mobility, and substantial healthcare costs due to surgical interventions. This study aimed to develop and evaluate Si-A/PUE@HA composite hydrogels as scaffolding materials for cartilage tissue engineering, addressing the limitations of current treatment options. Utilizing a multi-step synthesis method that incorporates both chemical and physical crosslinking techniques, we assessed the hydrogels’ cytocompatibility, chondrogenic differentiation potential, immunomodulatory properties, and in vivo cartilage regeneration capabilities. In vitro results demonstrated high cell viability and proliferation rates of bone marrow mesenchymal stem cells (BMSCs) cultured in Si-A/PUE@HA hydrogels, with significant expression of chondrogenic markers such as Acan, Sox9, and Col2a1. Notably, the hydrogels exhibited a favorable immunomodulatory effect, promoting an anti-inflammatory M2 macrophage phenotype, which is crucial for tissue regeneration. In vivo studies confirmed substantial new tissue formation and integration with surrounding cartilage, as evidenced by micro-CT analysis, alongside excellent biocompatibility with no significant adverse effects observed in major organs over a 12-week period. Single-cell RNA sequencing analysis revealed a favorable immune microenvironment and enhanced chondrogenesis linked to hydrogel treatment. In conclusion, Si-A/PUE@HA hydrogels represent a promising biomaterial with potential applications in cartilage repair and regenerative medicine, warranting further investigation in larger clinical trials to validate their efficacy and safety for future therapeutic use. Graphical abstract
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WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2025
·Vol. 41
·期 10
·1-17
·
IF 4.2
Li Jiaoqing,Huang Guohao,Zeng Jie,Wang Xiaodong,Wang Tan,Li Lin
The carbamate pesticide carbaryl poses significant ecological and health risks due to its widespread misuse and improper long-term disposal. In this study, we engineered a novel bacterial carbaryl-degrading esterase to enhance enzymatic activity, thermostability, and carbaryl degradation through semi-rational design and whole-cell biocatalysis strategies. An esterase, designated Est03320, was identified from Bacillus velezensis MB01B. Phylogenetic analysis revealed it resides in a distinct clade. However, it contains a catalytic domain similar to those of the well-characterized carbaryl-hydrolyzing esterase CarH and the chlorpyrifos-degrading esterase MPH. Est03320 was expressed and purified from Escherichia coli BL21(DE3) cells, demonstrating high hydrolytic activity against short-chain p -nitrophenyl esters and carbaryl. The enzyme exhibits an optimum pH of 6.5 and an optimal temperature of 25 °C, with a K m of 139.70 µM and a V max of 29.47 µM/min. The mutant Est03320-L230G, engineered through a combined semi-rational design and site-directed mutagenesis approach, exhibited a 68.7% increase in enzymatic activity toward the substrate p -nitrophenyl acetate, an extended half-life at 25 °C from 13.6 h to 40.6 h, and a 96.5% enhancement in carbaryl degradation efficiency. To construct a whole-cell biocatalyst, this mutant esterase was further fused with the autotransporter EhaA and displayed on the surface of engineered Pseudomonas sp. MB04R-14-03320-L230G-EhaA cells. The resulting biocatalyst showed high efficiency in degrading and mineralizing carbaryl, retaining over 83% of its activity after five consecutive degradation cycles. This integrated approach significantly enhances both enzymatic performance and reusability, offering a sustainable and effective approach for mitigating carbaryl pollution and advancing environmental protection.
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MOLECULAR NEUROBIOLOGY
·
2025
·Vol. 63
·期 1
·44
·
IF 4.3
Xie Min,Cheng Yu,Yang Lizhen,Lin Zijin,Shen Hao,Zhou Xinhua,Liu Zheng,Xing Jieyu,Zhou Sigui,Chen Haiyun
Parkinson’s disease (PD) is the second most prevalent neurological disorder without a clear etiology and specific cure. Microglia-mediated neuroinflammation plays a pivotal role in the pathogenesis and progression of PD, which is influenced by various factors and involves multiple signaling pathways. CT-011, synthesized by combining nitrone-tetramethyl pyrazine (TMP) and caffeic acid through an ester bond, has shown neuroprotection against ischemic stroke and glutamate-induced neuronal damage in animal models. The purpose of this study is to explore the impact of CT-011 on PD-related neuroinflammation and its potential in treating PD. Results showed that CT-011 significantly inhibited lipopolysaccharide (LPS)-induced release of pro-inflammatory cytokines and mediators in BV2 microglial cells. CT-011 also effectively mitigated LPS-induced mitochondrial membrane potential (MMP) reduction, mitochondrial and intracellular reactive oxygen species (ROS) production. Meanwhile, CT-011's anti-inflammatory effect was related to its inhibition of the TLR4-mediated MyD88/NF-B and PI3K-mediated AKT/GSK3β pathways. More significantly, CT-011 repressed the NLRP3 inflammasome activation. Additionally, CT-011 protected primary neurons against microglial-mediated neurotoxicity in vitro , and ameliorated dopaminergic neuronal damage in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice with a consistent anti-neuroinflammatory effect in vivo. These results demonstrate that CT-011 effectively inhibits microglia-mediated neuroinflammation and exerts neuroprotective effects on dopamine neurons, thus making it a promising therapeutic candidate for PD.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Tang Yanyan,Chen Qinglin,Zeng Mingmin,Ma Xiao,Zhu Ling,Zuo Zhibo,Lan Qingchun,Wu Yongquan
A significant limitation of existing fluorescent probes is their broad reactivity toward multiple biothiols, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). The structural similarity and overlapping reactivity of these thiol-containing species present a critical challenge for the selective discrimination of individual targets within complex biological environments. To address this limitation, we present M-Cys , a cysteine-selective frequency upconversion luminescence (FUCL) probe engineered from heteroanthracene-based fluorophores. By utilizing anti-Stokes emission, M-Cys enables near-infrared (NIR) excitation at 808 nm while simultaneously emitting NIR light (742 nm), thereby overcoming the limitations associated with conventional Stokes-shifted probes. This innovative design achieves a three-fold enhancement in sensitivity compared to traditional 690-nm excitation, with a detection limit of 23.2 nM, in contrast to 65.9 nM, attributable to reduced background interference and improved signal-to-noise ratios for FUCL. Comprehensive selectivity assays confirm M-Cys 's specificity for cysteine over competing biothiols, thereby ensuring reliable biological discrimination. In addition to in vitro experiments, M-Cys significantly enhances high-contrast imaging of exogenous cysteine in live cells and facilitates in vivo FUCL visualization in murine models. This methodology leverages FUCL's deep tissue penetration and minimal phototoxicity. By integrating unprecedented sensitivity with translational applicability, this study establishes a versatile optical platform that advances high-precision probes for clinical diagnostics. Graphical abstract
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·729
·
IF 12.6
Fu Weicong,Huang Tian,Wang Yi-Xin,Zhao Jing,Shen Jianliang,Yang Qining
Multidrug resistant (MDR) bacterial infections, particularly those involving methicillin-resistant Staphylococcus aureus (MRSA), pose a grave threat to global population health, necessitating novel therapeutic approaches capable of circumventing existing resistance mechanisms. Biomimetic enzymes, which generate bactericidal reactive oxygen species (ROS) by mimicking natural enzyme activity, represent a highly promising solution. This study reports a straightforward one-step synthesis method for vanadium dioxide (VO₂) nanoparticles, which function as potent biomimetic enzymes exhibiting both oxidase-like and peroxidase-like activities. When combined with near-infrared laser irradiation (808 nm, 1 W/cm²) for photothermal therapy (PTT), these VO₂ nanoparticles not only mediate local hyperthermia with a photothermal conversion rate of up to 36.9%, but also significantly enhance ROS generation through biomimetic catalysis. This achieves potent synergistic effects between photothermal therapy and chemodynamic therapy (CDT). This combined therapy exhibits potent antibacterial activity against suspended methicillin-resistant Staphylococcus aureus (MRSA) and effectively disrupts preformed biofilms. Furthermore, in a subcutaneous abscess mouse model, VO₂-mediated PTT-CDT treatment efficiently eradicated bacteria, alleviated local inflammation, promoted tissue repair and angiogenesis. In summary, this readily synthesised VO₂ nanozyme system offers an efficient and translatable therapeutic strategy for tackling challenging multidrug-resistant bacterial infections. Graphical
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Nano-Micro Letters
·
2020
·Vol. 12
·期 1
·1-15
·
IF 12.264
Miao Yalei,Qiu Yudian,Zhang Mengna,Yan Ke,Zhang Panke,Lu Siyu,Liu Zhongyi,Shi Xiaojing,Zhao Xubo
AbstractSection
Highlights
The formation of manganese oxide induces self-assembly of block copolymers to form polymeric vesicles.
The polymeric vesicles possessed strong stability and high drug loading capacity.
The drug-loaded polymeric vesicles have been demonstrated, especially in in vivo studies, to exhibit a higher efficacy of tumor suppression without known cardiotoxicity.
AbstractSection
Abstract
Molecular self-assembly is crucially fundamental to nature. However, the aqueous self-assembly of polymers is still a challenge. To achieve self-assembly of block copolymers [(polyacrylic acid– block –polyethylene glycol– block –polyacrylic acid (PAA 68 – b –PEG 86 – b –PAA 68 )] in an aqueous phase, manganese oxide (MnO 2 ) is first generated to drive phase separation of the PAA block to form the PAA 68 – b –PEG 86 – b –PAA 68 /MnO 2 polymeric assembly that exhibits a stable structure in a physiological medium. The polymeric assembly exhibits vesicular morphology with a diameter of approximately 30 nm and high doxorubicin (DOX) loading capacity of approximately 94%. The transformation from MnO 2 to Mn 2+ caused by endogenous glutathione (GSH) facilitates the disassembly of PAA 68 – b –PEG 86 – b –PAA 68 /MnO 2 to enable its drug delivery at the tumor sites. The toxicity of DOX-loaded PAA 68 – b –PEG 86 – b –PAA 68 /MnO 2 to tumor cells has been verified in vitro and in vivo. Notably, drug-loaded polymeric vesicles have been demonstrated, especially in in vivo studies, to overcome the cardiotoxicity of DOX. We expect this work to encourage the potential application of polymer self-assembly.
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Communications Earth & Environment
·
2026
·
IF 9.3
Wang Xuemeng,Li Lingyan,Li Huiju,Li Jie,Thies Janice,Xue Kai,Dong Xiuzhu
Wetlands are a significant source of methane, a potent greenhouse gas that contributes to global warming. Acetate and H 2 /CO 2 are generally considered the dominant precursors for methane formation, while H 2 , generally considered as the electron donor, is considerably lower in wetlands. This work investigated alternative reductive mechanisms of methanogenesis in the soil microcosms of Zoige wetland on Tibetan Plateau. Carbon monoxide inhibition of hydrogenase combined with isotope tracing experiments indicated only 2.9%-12.5% of the wetland H 2 was used in methanogenesis. Electron shuttles (biochar and magnetite) promoted methane production, and electrochemical experiments detected current generation from bacteria and current consumption coupled to methane production, confirming that electromethanogenesis occurred in the Zoige wetland and contributed 27.7%-50.9% of CH 4 production. Moreover, electromethanogenesis exhibited 2.6-16.1-fold higher CO 2 - and methanol-reductive methanogenic efficiencies than using H 2 . Uncultured Rice Cluster Ⅱ methanogens and a family of H 2 -dependent methylotrophic methanogen called Methanomassiliicoccaceae were enriched in electrochemical microcosms. Remarkably, oxidized anthraquinone-2,6-disulfonate decreased methane production. This work verifies the role of electromethanogenesis in a Tibetan wetland and provides a potential strategy for reducing methane emission.
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Science China-Materials
·
2026
·
IF 7.7
Zhang Jianhao,Jia Yi,Li Quan,Wang Yang,Pan Limei,Yang Jian
Development of flexible materials integrating broadband electromagnetic wave (EMW) absorption, infrared stealth, and hydrophobicity is required for complex environments. In this work, flexible SiTiOC ceramic fiber felts embedded with Sn nanoparticles were fabricated via electrospinning followed by in-situ carbothermal reduction. The metallic Sn promotes carbon graphitization, forming a three-dimensional conductive network and establishing heterogeneous interfaces within the SiTiOC matrix. First-principles calculations indicate that the work function gradient ( Φ Sn < Φ C ) drives electron migration at the Sn/C interface, inducing interfacial polarization, while turbostratic carbon in the SiTiOC phase provides defect dipole polarization. The fiber felt exhibits an effective absorption bandwidth of 7.3 GHz at a thickness of 3.75 mm. The material integrates radar-infrared compatible stealth through low thermal conductivity and low emissivity, with a water contact angle exceeding 138°. These results provide an interface engineering strategy for multifunctional stealth materials in complex environments.
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npj Vaccines
·
2026
·
IF 7.2
Yang Wenqi,Zhao Qingqing,Shi Wendi,Yan Jiacheng,Tang Di,Hu Yu,Zhang Linbo,Song Yueqi,Zhou Rui,Ren Qian,Li Yanyi,Wang Xintong,Cao Yufeng,Shi Li,Liang Zhihui,Xue Changying,Sun Bingbing
As a critical advancement in modern immunization strategies, multivalent vaccines combine multiple antigenic components to seamlessly broaden protection against diverse pathogens within a single dose. During the formulation of aluminum-adjuvanted vaccines, antigen adsorption onto the adjuvant surface serves as a critical determinant of vaccine performance. However, it remains unclear how the sequence of adsorption during formulation influences the particle-to-particle antigen loading heterogeneity on adjuvant particles and whether such differences are associated with immune outcomes. In this study, we systematically investigate how the adsorption sequence modulates particle-to-particle antigen-loading heterogeneity and antibody binding in bivalent vaccines. Using human papillomavirus (HPV16/18) and norovirus (NoV GI.1/GII.4) virus-like particles (VLPs) as model antigens with different adsorption strengths for amorphous aluminum hydroxyphosphate sulfate (AAHS), we demonstrate that the strength of antigen–adjuvant interaction is associated with how adsorption sequence influences antigen distribution. Antigens with weaker binding affinity exhibit distinct particle-to-particle antigen loading heterogeneity depending on the formulation sequence, whereas those with strong interactions remain largely unaffected. Importantly, these variations in particle-to-particle antigen-loading heterogeneity were associated with differences in antibody and monoclonal antibody binding, and with differences in in vivo humoral immune responses. These findings highlight adsorption sequence as an important formulation parameter associated with antigen distribution and antibody responses in multivalent vaccines.
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Scientific Reports
·
2026
·
IF 4.9
Wang Qi,Bi Pengfei,Wang Chunlin,Li Wenyang,Chi Bowen,Li Haitong,Tang Xin,Ma Tianyi
Colorectal cancer (CRC) often exhibits severe tolerance to immunotherapy due to the highly immunosuppressive nature of the tumor microenvironment (TME), with the polarization of tumor-associated macrophages (TAMs) toward the pro-tumor M2 phenotype playing a key role. Arginine (Arg), as a core precursor for the synthesis of the immune-microenvironment-reshaping metabolite spermine (Spm), is a crucial factor influencing the fate of TAMs. Unlike some tumors with Arg auxotrophy, CRC cells exhibited elevated argininosuccinate synthetase 1 (ASS1) expression, which was associated with increased extracellular Arg availability in our co-culture system. This study aimed to investigate the specific regulatory mechanisms of CRC cell metabolic reprogramming on immune function in the TME. Single-cell and clinical tissue analyses revealed elevated ASS1 expression in CRC epithelial cells and enrichment of SMS in M2-like TAMs, while higher spermine synthase (SMS) expression was associated with poorer survival in the analyzed PD-1-treated CRC cohort. The Transwell co-culture model demonstrated that CRC cells specifically upregulated ASS1 and released large amounts of Arg into the TME. Macrophages took up Arg and utilized their significantly enriched SMS to convert it into Spm. The intracellular accumulation of Spm further activated the PI3K-AKT-mTOR signaling pathway, driving macrophage polarization toward the M2 phenotype and upregulating immunosuppressive factors such as IL-10 and TGF-β, which in turn induced the exhaustion of local CD8 + T cells. Based on these findings, combined treatment with AMXT 1501 and Nivolumab attenuated M2-like polarization and the CD8⁺ T-cell exhaustion-associated phenotype, reduced CRC-cell proliferation, and increased apoptosis in vitro, with the major findings further validated in the MSS CRC cell line SW480. In conclusion, this study clarified the importance of the ASS1/Arg/SMS/Spm cross-cellular metabolic axis in CRC immune escape and demonstrated that targeting polyamine metabolism in combination with immune checkpoint blockade represents a promising strategy for overcoming immune tolerance across molecularly distinct CRC models.
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JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
·
2021
·Vol. 53
·期 3
·275-284
·
IF 2.945
Yang Rong,Li Xue,Mei Jie,Wan Wencheng,Huang Xinduo,Yang Qiaohong,Wei Xiaoyong
We have previously reported that syringic acid (SA) extracted from D. aurantiacum var. denneanum (kerr) may be used to prevent diabetic cataract (DC). However, the underlying mechanisms through which SA prevents DC in human lens epithelial cells (HLECs) remained unclear. In the present study, we employed single-molecule optics technologies, including transmission electron microscopy (TEM), atomic force microscopy (AFM), laser scanning confocal microscopy (LSCM) and Raman spectroscopy, to monitor the effect of SA on HLECs biomechanics and organelle structure in real-time. TEM suggested that SA improved the ultrastructure of HLECs with regard to nuclear chromatin condensation and reducing mitochondrial swelling and degeneration, which may aid in the maintenance of HLECs integrity in the presence of glucose. AFM revealed a reduced surface roughness and stiffness following SA treatment, suggesting an improved viscoelasticity of HELCs. Raman spectrometry and LSCM further revealed that these changes were related to a modification of cell liquidity and cytoskeletal structure by SA. Taken together, these results provide insights into the effects of SA on the biomechanics of HLECs and further strengthen the evidence for its potential use as a novel therapeutic strategy for DC prevention.
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