ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Chaohui Chen,Ningxing Li,Jingwen Lan,Xinghu Ji,Zhike He
A target-catalyzed hairpin assembly (CHA) and graphene/Au-NPs hybrids-based platform has been developed for the determination of DNA . This new sensor not only avoided any labeling but also reduced the background signal. In the absence of target, the assembly of H1 and H2 couldn't be triggered. The catalytic activity of graphene/Au-NPs hybrids was inhibited by adsorption of H1 and H2, leading to the “inactive” hybrids unable to catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). However, with the addition of target DNA, the target-catalyzed hairpin assembly was initiated and produced plenty of H1–H2 duplex, which had a weak binding affinity with the graphene/Au-NPs. Thus, the protected interface of graphene/Au-NPs hybrids became active and catalyzed the oxidation reaction of TMB accompanied with a colorless to-blue color change. This approach exhibited good sensitivity and specificity for target DNA with a detection limit of 5.74 × 10 −11 M, and realized the assay of target DNA in human serum samples. Besides, this sensor could be further expanded to detect viruses or proteins by adapting the corresponding aptamers, showing great potential in biochemical detections.
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高影响因子
RSC Advances
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2015
·Vol. 5
·期 40
·31262-31269
·
IF 3.84
Yulan Wang,Yueyun Li,Hongmin Ma,Xiang Ren,Wei Cao,Tao Yan,Qin Wei
A simple and novel label-free electrochemical immunosensor based on a bimetallic alloy was fabricated in this work. Three-dimensional (3D) pine-like Au–Cu nanodendrites (NDs) electrodeposited on the bare glassy carbon electrode (GCE) were employed as transducing materials to achieve the production and amplification of the electrochemical signal. The electrochemical detection was based on the fact that the immunocomplex can hinder the direct electron transfer in the redox process of Cu. Cu, instead of the traditional redox electron mediators, was exploited as a new signal producer. Au with good electron transfer ability could act as signal amplifier and capture antibodies. In addition, the 3D pine-like morphology of Au–Cu NDs with large specific surface area was beneficial to the immobilization of antibodies and the electron transfer between protein and metal. Under the optimal conditions, by using carbohydrate antigen 724 (CA724) as the target analyte, the label-free electrochemical immunosensor exhibited a wide linear range from 0.5 pg mL−1 to 500 ng mL−1 and a low detection limit of 0.25 pg mL−1. The proposed immunosensor also displayed a satisfying electrochemical performance with good reproducibility, selectivity and stability. In this sense, this novel method will provide a new promising application for the clinical immunoassay of other biomarkers.
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Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Jingyu Pang,Linan Zhou,Zhuang Liang,Tingting Li,Rui Yan,Fang Chai
The determination of heavy metal ions is critical to both human health, ecosystems, and the environment because it is a prerequisite for separating heavy metal pollutants. Herein, a ratiometric fluorescent strategy based on bimetallic AuAg nanoclusters and Si nanoparticles (AuAg-SiNPs) was constructed and achieved multivariate detection of Ag + , Cu 2+ , and Hg 2+ , simultaneously. The AuAg-SiNPs exhibited dual emission centered at 456 nm and 631 nm, with quantum yield of 23.48 %, and presented visual distinguishable signal for different ions. Notably, a smartphone visual platform was created to identify Ag + , Cu 2+ , and Hg 2+ with portable and accurate capturing and recognizing changing RGB values. The quantitative evaluation can be confirmed by various noticeable ratiometric evolution responses to three ions, the limits of detection were 1.61 nM, 49.94 nM, and 1.44 nM for Ag + , Cu 2+ , and Hg 2+ , respectively. Excellent environmental tolerance as well as reliability and practicability of AuAg-SiNPs enabled their test in real water samples. The synergistic effect of aggregation-caused quenching, static quenching effect, internal filtration effect, and fluorescence resonance energy transfer were revealed in process of detection. Ratiometric fluorescent probes offer great advantages in multiple identification of heavy metals in real sample. Smartphone-assisted detection greatly improves the effectiveness of visual on-site detection and has a promising application in real samples.
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Biomaterials Advances
·
2024
·
IF 5.5
Wuming Wu,Xiaolian Jiang,Qin Zeng,Huiyu Zou,Chunyan Deng
In this study, epigallocatechin-3-gallate nanospheres (EGCG NSs) are employed as an innovative alternative to traditional reducing agents for the in-situ growth of AuNPs on the EGCG NS surface to produce the Au nanoparticles decorated EGCG nanospheres (EGCG NS@AuNPs). This eco-friendly approach avoids toxic chemicals and simplifies the synthesis process, enhancing biocompatibility and functional properties of the resulting EGCG NS@AuNPs nanocomposite. The nanocomposite exhibits remarkable stability, photothermal properties, and peroxidase-like enzymatic activity. Taking advantage of the enhanced photothermal properties, the application of EGCG NS@AuNPs in the antibacterial field was investigated, and the antibacterial activity was greatly improved against both Gram-negative and Gram-positive bacteria comparing to bare AuNPs or EGCG Ns. Additionally, based on the excellent enzymatic activity, the sensing application of EGCG NS@AuNPs was explored by developing a colorimetric method for detecting ascorbic acid (AA). A remarkably low detection limit of 0.076 μM was achieved. This method has been successfully applied to measure the AA content in vitamin C tablets, demonstrating the practicality and accuracy of this approach. Therefore, the synthesis for EGCG NS@AuNPs is not only rapid, and cost-effective, but also eco-friendly and not harmful to biological systems, which is potential in biosensing, clinical diagnosis, and therapeutics. Future research could explore further applications of EGCG NS@AuNPs in biomedicine field, revealing even more of its remarkable potential.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Peng Wang,Chengli Tang,Libing Zhang,Yebo Lu,Fengli Huang
Ammonia (NH 3 ) gas is regarded as a biomarker for clinical diagnosis of various diseases and a potential indicator for the spoilage of protein-rich foods. The acuate detection of the ammonia gas concentration depend on reliable gas sensor. In this paper, flexible room temperature ammonia gas sensor based on hierarchical Au/PANI/WS 2 ternary composite was successfully prepared by in-situ polymerization, reduction and drop-coating process. The Au/PANI/WS 2 -based ammonia sensor was tested for its room temperature sensing property including response value, response/recovery time, selectivity, and long-term stability. The effects of flexible state, humidity and operating temperature on the sensor response were also investigated. The 0D/1D/2D Au/PANI/WS 2 -based sensor showed significantly enhanced ammonia sensing property both in response and response/recovery time as compared to that of pristine WS 2 . Gas sensing mechanism of the prepared sensor was investigated by complex impedance spectroscopy and in-situ fourier transform infrared spectroscopy analysis. Besides, the sensor was proved for its practical usability in seafood spoilage detection and warning. The construction of multidimensional materials into hierarchical structure may provide an instructive way for the sensibilization of layered two-dimensional nanomaterial of WS 2.
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TALANTA
·
2024
·
IF 5.6
Kaida Kuang,Yao Lu,Yang Chen,Pei Zhang,Nengqin Jia
The traditional luminol electrochemiluminescence (ECL) sensing suffers from low signal response and instability issues. Here, an Au/ZnCuS double-enhanced g -C 3 N 4 -supported luminol ECL aptasensor is constructed for the sensitive detection of human mucin 1 (MUC1). In this platform, g -C 3 N 4 of a large specific surface area is beneficial to load more luminol illuminants. Au nanoparticles promote the decomposition of H 2 O 2 coreactants to generate more reactive oxygen (•OH and O 2 • − ) intermediates, while ZnCuS can immobilize the aptamer and simultaneously catalyze H 2 O 2 decomposition, realizing the double-wing signal amplification. Under optimal conditions, this sensor shows a good detection capability within 1.0 × 10 −4 –1.0 × 10 3 ng mL −1 and a low detection limit of 5.0 × 10 −5 ng mL −1 , as well as ideal stability, selectivity, and reproducibility. This double-enhanced aptasensor highlights a new signal-enhancement approach for early biomarker detection.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
·
IF 4.3
Haifeng Yao,Changbo Jia,Yongping Dong
Herein, a novel sandwich electrochemiluminescence (ECL) aptasensor was developed based on the resonance energy transfer (RET) with iridium complex doped silicate nanoparticles (SiO 2 @Ir) as energy donor and gold nanoparticles modified TiVC MXene (AuNPs@TiVC) as energy acceptor. Strong anodic ECL signal of SiO 2 @Ir was obtained through both co-reactant pathway and annihilation pathway. Electrochemical results showed that SiO 2 @Ir has good electron transfer rate and large specific surface area to immobilize more aptamers. AuNPs@TiVC apparently quenched the ECL signal of SiO 2 @Ir due to the ECL resonance energy transfer between them. In the presence of kanamycin (KAN), a sandwich type sensor was formed with the aptamer probes as connecters between the donor and the acceptor, resulting in the decrease of ECL intensity. Under the optimal condition, KAN could be sensitively detected in the range of 0.1 pg/mL to 10 ng/mL with a low detection limit of 24.5 fg/mL. The proposed ECL system exhibited satisfactory analytical performance, which can realize the detection of various biological molecules by adopting suitable aptamer.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Sicheng Xu,Hanyan Zhang,Zhiyi Qian,Weizhong Yuan
A novel and efficient cancer therapy was developed using a smart hydrogel containing multifunctional bimetallic organic frameworks and anticancer drugs. The injectable self-healing hydrogel with pH-responsiveness was constructed through borate ester and imine bonds among dopamine-grafted sodium alginate (SADA), hydroxypropyl chitosan (HPCS) and 2-formylphenylboronic acid (2-FPBA). The Au nanoparticles-decorated Ti/Fe bimetallic organic framework tetragonal nanosheets (Au/TF-MOF TNS) were synthesized and incorporated into the hydrogel with the anticancer drugs doxorubicin (DOX). Upon intratumoral injection of nanocomposite hydrogel, the acidic tumor microenvironment triggered the cleavage of borate ester and imine bonds, causing the hydrogel to break down and accelerating the release of both Au/TF-MOF TNS and DOX. These Au/TF-MOF TNS functioned as nanozymes, producing hydroxyl radicals (·OH) for chemodynamic therapy (CDT), generating oxygen (O 2 ) to support sonodynamic therapy (SDT), and depleting glucose for starvation therapy (ST). Additionally, the Au/TF-MOF TNS served as sonosensitizers, capable of converting O 2 into singlet oxygen ( 1 O 2 ) upon ultrasound irradiation to achieve SDT. Therefore, this nanocomposite hydrogel system enabled synergistic sonodynamic-chemodynamic-starvation-chemo therapy (SDT-CDT-ST-CT) of cancer, presenting a promising platform for advanced cancer therapy strategies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xi Jin,Liangke Gong,Jianwei Liang,Zhenhao Wang,Kuiyu Wang,Tao Yang,Hui Zeng
Vertically-ordered mesoporous silica film (VMSF) has proven to be an effective anti-interference and anti-fouling layer in the field of analysis. However, because VMSF is not stable enough in complex samples, its application in sensing field is limited. We herein use mussel-inspired multi-functional agent polydopamine (PDA) to enhance the stability of VMSF on electrode. Owing to the amino-functionalized VMSF combined with amino groups on PDA, superhydrophilicity was achieved which was proved to enhance excellent anti-fouling property. In addition, the electrodeposition of Au nanoparticles (AuNP) in the nanochannel of VMSF serving as an internal electrochemical signal shows excellent electrochemical signal stability, which provides a prerequisite for maintaining the further detection accuracy in complex samples. Finally, we validated the detection performance of the anti-fouling sensing strategy using a foodborne pathogen, Vibrio parahaemolyticus (VP), as a model target. Specifically, the aptamer was modified by covalent bond on the PDA-integrated VMSF interface surface for the recognition of VP. Using shrimp homogenate as a real sample, the anti-fouling detection performance of the aptasensor was verified. The linear range of VP detection in real samples was 10 3 -10 7 CFU/mL, and the detection limit was 10 3 CFU/mL. The results show that the anti-fouling aptasensor has excellent anti-interference and stability. It is worth emphasizing that this strategy (using inherent Au signal) enables rapid electrochemical analysis of VP without outer indicators, greatly reducing the difficulty of constructing an electrochemical VP detection strategy.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Yahui Zhang,Xia Huang,Jiachang Chen,Jiyuan Li,Shen Zhou,Junying Chen
Quantitative analysis of harmful patina components on the surface of bronzeware is crucial as patina can corrode artifacts. Surface-enhanced Raman spectroscopy (SERS) has a common application in surface analysis. However, there exists an absence of documented investigations concerning the quantitative analysis of detrimental patina, particularly the chloride patina. To achieve high sensitivity in targeting harmful patina, we report a fabrication method for constructing Ag/Au alloys in the pores and on the surface of UiO-66 (zirconium 1,4-dicarboxybenzene metal–organic framework) nanomaterials, resulting in Ag/Au/UiO-66 nanoparticle monolayers formed by self-assembly at the oil–water interface. By incorporating them onto the surface of PVA hydrogel, we obtained SERS sensor devices with excellent flexibility, outstanding optical transparency, and ultrahigh activity. These sensor devices efficiently sample irregular surfaces and detect micrometer-scale specimen. They significantly enhance the Raman characteristic peaks of measured objects, facilitating precise analysis of the patina composition on the surface of bronze artifacts. The Raman peak at 118 cm–1 was boosted from 367 to 11,909 for detecting harmful patina, and the enhancement factor of the SERS substrate was calculated to be 3.6 ×1010 for detecting the Raman peak of rhodamine 6G (R6G), which could be easily detected at 610 cm–1 at a concentration of 10–13 mol/L (M). The Raman peaks at 610 cm–1 from the SERS substrate could also be readily detected. By grafting the organic fluorescent group amino-MQAE onto the surface of Ag/Au/UiO-66 nanoparticles (NPs), the content of harmful patina on the surface of bronze artifacts treated for different durations was indirectly determined. These results indicate the successful construction of the Ag/Au alloy on the surface of UiO-66 NPs, resulting in SERS sensor devices with excellent flexibility, optical transparency, and ultrahigh sensitivity.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Shengqiao Gao,Qingyi Wei,Hongbin Pu
Heterojunction-like substrates have drawn significant attention in surface enhanced Raman spectroscopy (SERS) owing to their multi-enhancement characteristics. In this study, the core-shell architectures of UiO-66@ TiO 2 were fabricated for capture and enrichment of organophosphorus pesticides (OPs) in solution. After absorption of OPs, the SERS-active nanoparticles of Au were assembled on the core-shell architectures to form UiO-66@TiO₂/Au substrate via Au-S bonding between OPs molecule and AuNPs and electrostatic interactions between UiO-66@TiO 2 and Au, resulting in adsorption-induced aggregation of Raman signal for OPs. The proposed method of adsorption-induced aggregation could be used for detecting thiamethoxam and triazophos in apple juice with detection limit of 3.51 μg/L and 0.66 μg/L, respectively. Furthermore, the satisfactory recovery rates, ranging from 91.98 % to 104.31 % for thiamethoxam and from 91.47 % to 116.47 % for triazophos, indicated that the prepared heterojunction-like substrate could be potentially used for trace detecting OPs in foods.
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Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 9
·3254-3260
·
IF 5.7
Dandan Men,Hong Wang,Qianqian Ding,Yingyi Wu,Ting Wu,Wenshan Qu,Liang Ma,Honghua Zhang,Guihua Jiang,Lifeng Hang
In numerous studies, it is imperative to establish a homogeneous refractive index (RI) surrounding environment to acquire a surface lattice resonance (SLR) characteristic peak for a plasmonic array. However, this requirement will limit the practical applicability of the SLR. This study successfully achieved the fabrication of cost-efficient, size-adjustable, and structure-transferable two-dimensional (2D) ordered gold (Au) near-spherical nanoparticle (NP) arrays with large NP size (>100 nm) through utilizing a colloidal crystal template and annealing process. In this method, the NP diameter can be adjusted by varying the thickness of the deposited Au film. Importantly, the fabricated 2D Au NP arrays with larger NP size supported an obvious and sharp SLR peak by exciting with normal incident light in an asymmetric air environment. These results indicated that if the arrays were well arranged and the NP size was large enough, index-matching was not necessary for the formation of far-field electromagnetic coupling of the plasmonic NPs. Furthermore, theoretical calculation demonstrated that the significant SLR signal in air environment was relevant to the NP size. Due to the obvious SLR peak in air, the 2D Au NP arrays were applied as a sensor to detect hemoglobin (Hb). It was found that the SLR peak was more sensitive than the localized surface plasmon resonance (LSPR) peak, and its offset was obviously observed when the Hb concentration was as low as 10 μM. Moreover, the Au NP arrays also displayed good uniformity and stability. Besides rigid nanophotonic sensor, the prepared 2D Au NP arrays on a quartz substrate can be transferred to a flexible substrate to form a flexible free-standing optical sensor, providing a real-time tunable SLR mode. With the decrease of water content, the SLR peak continuously blue-shifted from 1058 nm to 605 nm, displaying high sensitivity. This paper not only provides a cost-effective and controllable approach to fabricate next-generation rigid and flexible nanophotonic devices, but also paves the way for practical application of SLR in sensing.
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