JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 101
·期 7
·2779-2787
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IF 2.614
Wenbi Guan,Yingxin You,Jialing Li,Jiaying Hong,Haiying Wu,Yanna Rao
BACKGROUND Penicillic acid (PA) is produced by Aspergillus spp. and Penicillium spp., which are common postharvest and storage fungi of fruits. PA can be of concern for human health because of its toxicity and high fruit consumption by the population. However, no data on PA occurrence in various fruits have yet been reported. A quick, easy, cheap, effective, rugged and safe (QuEChERS) approach for PA determination in various fruits was developed and applied to explore PA incidence in fruits. RESULTS The modified QuEChERS procedure with extraction by ethyl acetate and purification by multi-walled carbon nanotubes (MWCNTs), primary secondary amine (PSA) and octadecyl silane (C18) was established to determine PA in various fruits by high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS). The average recoveries were 72.9–102.2% and relative standard deviations (RSDs) were 1.3–7.9%. A total of 161 fruits samples, including kiwi, apple, peach, grape and mandarin/orange, were collected in southern China. The incidence of PA in fruits was 14.9% and the levels of PA contamination were 0.200–0.596 μg kg −1 . Our results suggested that orange/mandarin, grape and kiwi were favorable matrices for Aspergillus spp. and Penicillium spp. to produce PA, rather than peach and apple. CONCLUSION To the best of our knowledge, this is the first report about PA contamination in various fruits in China. Our study emphasizes the necessity of the current established method, which could be used for continuous monitoring of PA and reducing the health risk to Chinese consumers. © 2020 Society of Chemical Industry
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MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Dan Qiao,Zhen Zhang,Lingshuang Wang,Wei Sheng,Qiliang Deng,Shuo Wang
Test strips as one of micro-devices are widely concerned in the fields of point-of-care detection and in-field diagnosing. For the purpose of on-site detection of tyramine, a carbon dots-molecularly imprinted fluorescent sensing test strip with high selectivity has been successfully obtained via a rapid in-situ preparation procedure (about 2.5 h). Here, the layer composed of carbon dots-molecularly imprinted polymer (CDs-MIP) with the function of the recognition and signal transduce has been formed on the surface of the filter paper strips via the sol-gel method. The developed test strip displayed a high specificity (Imprinting factor, IF = 4.96); and its fluorescence emission was quenched linearly with the increase of tyramine concentration from 0.5 to 10.0 mg L −1 . The limit of detection (LOD) is 0.059 mg L −1 (3 σ/s ). The application potential was verified by sensing of tyramine in rice vinegar and white rice vinegar with the recoveries of 90.9-104.4% (RSD < 5.8%). Many advantages including a rapid preparation and high selectivity make the fluorescence strip suitable for on-site detection of tyramine. In addition, the preparation procedure can also be easily transferred to the preparation of the fluorescence strip toward other targets.
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BIOSENSORS & BIOELECTRONICS
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2020
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IF 10.257
Bing Han,Lingjun Sha,Xiaomeng Yu,Mei Yang,Ya Cao,Jing Zhao
Breast cancer is the most common malignant disease among women worldwide. Nowadays, combined therapy against several therapeutic targets is becoming a promising treatment to enhance the survival rate of the patients with some lethal subtypes, and also proposes high demand on the discrimination of the co-existing targets in breast cancer. In this work, we designed in situ automatous DNA assembly reaction and applied it for the simultaneous identification of dual therapeutic targets using electrochemical techniques . Taking triple-negative breast cancer cell MDA-MB-231 as a model, chained strand displacement reactions were initiated after the capture probes recognized the surface biomarkers, epidermal growth factor receptor and intercellular adhesion molecule-1, respectively. Then, an increased electrochemical signaling was created to reveal the co-expression of the two targets using quantum dots as electrochemical labeling. Electrochemical results demonstrated high sensitivity and specificity of our method toward the identification of the coexisted therapeutic targets even in the serum samples, which also allowed to monitor the enhanced efficiency of combined therapy. Therefore, our method suggested a potential use in the accurate identification of therapeutic targets in breast cancer that might provide more information to facilitate the combined therapy in clinic.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2021
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IF 5.571
Xiao Peng Zhang,Bei Bei Wang,Wen Xuan Li,Wen Min Fei,Yong Cui,Xin Dong Guo
Dissolving microneedles (DMNs) are widely used in drug delivery systems since they are based on one-step application, which is simple and convenient for patients, especially for the patients such as diabetes who need daily or long-term self-administration. In general, the matrix materials of DMNs are water-soluble materials that can release the encapsulated drugs gradually by dissolving in the skin without generating sharp needle waste. However, the matrix materials of DMNs will also leave in the skin after application. Thus, it is vital to evaluate whether the matrix material of DMNs dissolved in the skin will cause health risks such as toxicity to the body or some skin-related complications to patients who frequent or long-term administration. In this work, PVA , as one of the typical matrix materials of DMNs, was selected to prepare the DMNs to research the safety of PVA-based MNs to the body after being dissolved in the skin. Briefly, in a 160 – days trial, the healthy mice were daily administrated by PVA MNs. The results showed that PVA materials mainly accumulated in the skin tissues of mice after dissolving and the concentration of PVA in the insertion sites gradually decreased and was almost undetectable at 6 days after administration. The observation of general conditions, blood hematological analysis and histological examinations of the mice demonstrated that the PVA-based MNs do not cause appreciable toxicity to the healthy mice after daily insertion in a 160 – days trial. Altogether, these results encourage further studies of PVA MNs for biomedical applications and support translation of PVA-based DMNs from pre-clinical development into clinical trials .
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CHEMICAL ENGINEERING JOURNAL
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2020
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IF 10.652
Yanyan Liu,Qiankun Zhao,Jun Yue,Chaoqun Yao,Guangwen Chen
The mass transfer of slug flow, a widely applied flow pattern in microreactors, is still difficult to predict mainly due to the competing nature between convection and diffusion. This work focused on the influence of the mixing mechanism on the mass transfer performance under gas–liquid and liquid–liquid slug flow, in both continuous slugs and dispersed droplets. Colorimetric study with the resazurin oxidation system was implemented, where the mass transfer resistance was constantly located in the aqueous phase. In the hydrophilic glass microreactor, the convection featured by intensive internal circulation and/or inter-slug leakage flow dominated diffusion, leading to nearly-constant k L a along the channel under given flow rates. However, in the hydrophobic PTFE capillary, the stagnant region constituted a significant share in the aqueous droplet, indicating the prominent role of diffusion against convection therein. As a result, k L a values decreased along the main channel length in fixed operating conditions. Accordingly, prediction models were respectively correlated depending on mixing mechanisms. Moreover, mass transfer contributions from the bubble and droplet formation stages were also investigated. This work is expected to shed light on judicious process design and reliable predictions in microreactor operations.
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BMC Ophthalmology
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2020
·Vol. 20
·期 1
·1-10
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IF 1.413
Yan Weiming,Long Pan,Wei Dongyu,Yan Weihua,Zheng Xiangrong,Chen Guocang,Wang Jiancong,Zhang Zuoming,Chen Tao,Chen Meizhu
Background Retinitis pigmentosa (RP) is a kind of inherited retinal degenerative diseases characterized by the progressive loss of photoreceptors. RP has been a conundrum without satisfactory countermeasures in clinic until now. Acetaldehyde dehydrogenase 2 (ALDH2), a major enzyme involved in aldehyde detoxification, has been demonstrated to be beneficial for a growing number of human diseases, such as cardiovascular dysfunction, diabetes mellitus and neurodegeneration. However, its protective effect against RP remains unknown. Our study explored the impact of ALDH2 on retinal function and structure in N-methyl-N-nitrosourea (MNU)-induced RP rats.Methods Rats were gavaged with 5 mg/kg Alda-1, an ALDH2 agonist, 5 days before and 3 days after MNU administration. Assessments of retinal function and morphology as well as measurement of specific proteins expression level were conducted.Results Electroretinogram recordings showed that Alda-1 administration alleviated the decrease in amplitude caused by MNU, rendering protection of retinal function. Mitigation of photoreceptor degeneration in MNU-treated retinas was observed by optical coherence tomography and retinal histological examination. In addition, Western blotting results revealed that ALDH2 protein expression level was upregulatedwith increased expression of SIRT1 protein after the Alda-1 intervention. Besides, endoplasmic reticulum stress (ERS) was reduced according to the significant downregulation of GRP78 protein, while apoptosis was ameliorated as shown by the decreased expression of PARP1 protein.Conclusion sTogether, our data demonstrated that ALDH2 could provide preservation of retinal function and morphology against MNU-induced RP, with the underlying mechanism at least partly related to the modulation of SIRT1, ERS and apoptosis.
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Nanoscale
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2020
·Vol. 12
·期 32
·17113-17120
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IF 6.895
Ziren Xie,Xingjun Li,Renfu Li,Shan Lu,Wei Zheng,Datao Tu,Yanhui Feng,Xueyuan Chen
The metal halide perovskite quantum dots (APbX3 PeQDs; A = Cs or CH3NH3; X = Cl, Br or I) have emerged as a new type of promising optoelectronic material for light-emitting and photovoltaic applications because of their excellent optical properties. However, the precise control over the size and photoluminescence (PL) emission of APbX3 PeQDs remains a great challenge, which has been one of the main obstacles to the applications of PeQDs. Herein, we report a unique strategy for in situ confined growth of MAPbBr3 (MA = CH3NH3) PeQDs by using porous metal–organic framework (MOF) UiO-66 as a matrix. By introducing Pb(Ac)2 and MABr precursors into the pores of UiO-66 via a stepwise approach, ultrasmall MAPbBr3 PeQDs were in situ grown in the matrix with the size tuned from 6.4 to 3.3 nm by changing the concentration of the Pb(Ac)2 precursor. Accordingly, the PL emission wavelength of the resulting MAPbBr3 PeQDs was blue-shifted from 521 to 486 nm with the size reduction, owing to the strong quantum confinement effect of the PeQDs. Due to the surface passivation effect endowed by the UiO-66 matrix, the ultrasmall MAPbBr3 PeQDs also displayed a high PL quantum yield (PLQY) of 43.3% and a long PL lifetime of 100.3 ns. This study proposes a new strategy to prepare ultrasmall PeQDs and effectively control their sizes and PL emissions, which may open up new avenues for the development of high-performance luminescent PeQDs for diverse applications.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2020
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IF 2.734
Chao Luo,Weibin Wu,Shuaijie Lou,Saiqi Zhao,Kai Yang
Tanshinone IIA (TA) is a major active ingredient of traditional Chinese medicine Salvia miltiorrhiza Bunge (Danshen) with poor solubility. The strong crystallinity of TA is the main reason for its poor solubility. Since sodium alginate (SA) can suppress the drug crystal growth and has excellent biocompatibility , we hypothesize that SA may be an ideal matrix for preparing solid dispersion (SD) to improve the dissolution rate and bioavailability of TA. In this study, a novel TA-SA-SD was developed via a rapid one-pot approach for improving the dissolution rate, in vivo bioavailability, and pharmacological activity of TA. The results of scanning electron microscopy (SEM), X-ray diffraction (XRD) confirmed the SD process will reduce the crystallinity of TA. The crystallinity reduction of TA in TA-SA-SD can obviously improve the dissolution rate, oral absorption rate and in vivo bioavailability, the relative bioavailability of TA-SA-SD (1:6) and TA-SA-SD (1:8) were 2.08 and 2.75 folds of TA, respectively. In vitro anti-inflammatory assays were performed in RAW264.7 cells by ELISA , fluorescence microscope and Western blot analysis . The results revealed that the TA-SA-SD can significantly improve the pharmacological activity of TA. This study provides a successful method of incorporating hydrophobic drugs into hydrophilic bio-macromolecules matrices for improving the dissolution rate and bioavailability.
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JOURNAL OF EXPERIMENTAL BOTANY
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2020
·Vol. 71
·期 10
·3066-3079
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IF 5.908
Liu Xiaojuan,Zhao Chenning,Gong Qin,Wang Yue,Cao Jinping,Li Xian,Grierson Donald,Sun Chongde
Polymethoxylated flavones (PMFs), which accumulate exclusively in fruit peel of citrus, play important physiological and pharmacological roles but the genetic basis for the methylation of flavonoids has not been fully elucidated in citrus. Here we characterize a caffeoyl-CoA O-methyltransferase-like enzyme, designated CrOMT1. The expression pattern of CrOMT1 was highly correlated with the concentration of the three major PMFs in two different citrus fruit tissues during fruit maturation. Exposure of fruit to UV-B radiation sharply increased the level of CrOMT1 transcripts and also led to the accumulation of three PMFs. The potential role of CrOMT1 was studied by testing the catalytic activity of recombinant CrOMT1 with numerous possible substrates in vitro. The enzyme could most efficiently methylate flavones with neighboring hydroxy moieties, with high catalytic efficiencies found with 6-OH- and 8-OH-containing compounds, preferences that correspond precisely with the essential methylation sites involved in the synthesis of the three naturally occurring PMFs in Citrus reticulata. This indicates that CrOMT1 is capable of in vitro methylation reactions required to synthesize PMFs in vivo. Furthermore, transient overexpression of CrOMT1 increased levels of the three major PMFs in fruit, indicating that CrOMT1 is likely to play an essential role in the biosynthesis of PMFs in citrus.
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CHEMOSPHERE
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2020
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IF 5.778
Jian Cheng,Doudou Wang,Baohua Wang,Han Ning,Ying Zhang,Yuchao Li,Jiutao An,Peiling Gao
The α-MnO 2 , β-MnO 2 and γ-MnO 2 samples were prepared by the hydrothermal method and were used for the degradation of ciprofloxacin (CIP) wastewater in a combined DBD-catalytic process. The physical and chemical properties of the samples were systematically studied by several analytical techniques including BET, XRD, SEM, HRTEM, XPS, and H 2 -TPR. The combination of DBD with α-MnO 2 showed the highest CIP degradation efficiency, and the efficiency could reach 93.1% after 50 min, which was 10.8% and 18.1% higher, respectively, than those of β-MnO 2 and γ-MnO 2 catalysts in the plasma-catalytic system. According to the model of response surface methodology , the contribution of key experimental parameters on the CIP degradation decreased in the order: peak voltage > air flow rate > initial concentration > initial pH. The optimum operating parameters were peak voltage 17 kV, air flow rate 2.5 L min −1 , an initial concentration 5 mg L −1 and an initial pH 6.9. The quenching experiments of active species showed that OH and O 2 − were critical to the CIP degradation. The generated O 3 might be adsorbed by the α-MnO 2 catalyst and resulted in more OH generation. The intermediate products of CIP degradation in DBD+α-MnO 2 system were analyzed by LC-MS, and three possible degradation pathways were proposed. This research provides an insight into the use of the crystallographic structures in discharge plasma system for antibiotics in water.
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JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Liangchen Yue,Jun Cheng,Haihua Zhang,Luyun Yuan,Junjie Hua,Haiquan Dong,Yu-you Li,Junhu Zhou
To study the inhibited degradation metabolism and anaerobic digestion of typical lipids in food waste, an artificially produced capsaicin, N-Vanillylnonanamide, a typical soluble component in waste lipids, was added to a glycerol trioleate anaerobic digestion system. The microorganisms damage and blocked electron transfer caused by N-Vanillylnonanamide during anaerobic digestion were further clarified. Scanning electron microscopy and transmission electron microscopy images demonstrated that N-Vanillylnonanamide (≥4 wt%) structurally damaged microorganisms via cell membrane breakage, which impair their function. N-Vanillylnonanamide inhibited the activities of the key enzyme CoA, AK, F 420 , and CoM, which are relevant for both degradation metabolism and anaerobic digestion. 16S rRNA analysis showed that dominant bacterial and archaeal communities markedly decreased after anaerobic digestion of glycerol trioleate with N-Vanillylnonanamide (≥4 wt%). For example, the proportion of Methanosarcina decreased from 30 % to 6 %. Current-voltage curves indicated that the electron transfer rate in the community of microorganisms decreased by 99 % from 4.67 × 10 −2 to 5.66 × 10 −4 s −1 in response to N-Vanillylnonanamide (40 wt%). The methane yield during anaerobic digestion of glycerol trioleate decreased by 84.0 % from 780.21–142.10 mL/g-total volatile solids with N-Vanillylnonanamide (40 wt%).
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JOURNAL OF MEMBRANE SCIENCE
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2020
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IF 7.183
Ruoxi Wang,Xueting Zhao,Youyou Lan,Lifen Liu,Congjie Gao
Superhydrophilic and underwater superoleophobic membranes have recently attracted increased attention in oily wastewater treatment because of their potential to promote membrane permeability and minimize membrane fouling . Despite recent advances, the manipulation of membrane superwettability is usually achieved by coating functional layers on commercial hydrophobic porous substrates. The direct fabrication of superhydrophilic and underwater superoleophobic microporous membranes still remains a challenge. In this work, we provide a new in situ metal-polyphenol interfacial assembly strategy and prepare a new type of superwetting PES/SPES/MPN membranes. In the phase inversion of membrane formation, the coordination assembly of tannic acid (TA) and transition metal ions takes place alone with the solvent/nonsolvent exchange, resulting in the in situ deposition of metal-polyphenol networks (MPNs) on as-prepared polyether sulfone (PES)/sulfonated polyether sulfone (SPES) membranes. The PES/SPES/MPN membranes exhibit hierarchical surfaces and bi-continuous pores, and the in situ deposited MPNs provide hydrophilic bases. Among them, the PES/SPES/TA-Fe 3+ membrane showed the highest superhydrophilicity and underwater superoleophobicity. Thanks to the excellent superhydrophilicity, the water flux of PES/SPES/TA-Fe 3+ membrane can reach 4423 Lm −2 h −1 . Moreover, the PES/SPES/TA-Fe 3+ membrane exhibits high-performance for oil-in-water emulsion separation with high flux (>1500 Lm −2 h −1 ), excellent oil rejection (>99.5%) and reliable enhanced antifouling and recycling performance. This study provides a novel and facile strategy for the direct fabrication of superwetting microporous membranes, and predicts the potential application of PES/SPES/MPN membranes in oily wastewater treatment.
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