JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Shuyu Tang,Hongxi Zhang,Ji Li,Jun Huang,Liang Wei,Jing Yang,Xiande Yang
The valorization of lignin into valuable chemicals is a cornerstone of future biorefineries, but it is often hindered by the need for harsh reaction conditions. Electrochemical oxidation offers a promising green alternative, yet many systems lack mechanistic clarity, which impedes rational design. Herein, we report a simple and highly efficient electrochemical system using tetrabutylammonium tetrafluoroborate (TBABF4) in methanol for the selective cleavage of lignin’s β-O-4 ether bond under ambient conditions. On a model compound, this metal-free system achieved over 97% conversion with excellent selectivity toward high-value aldehyde products. Mechanistic studies revealed a dual-pathway mechanism. Crucially, when this mechanistically guided protocol was applied to bagasse lignin, it demonstrated effective depolymerization, leading to the significant cleavage of native β-O-4 bonds, resulting in a total liquid product yield of 15.6%, which included valuable aromatic products such as benzaldehyde dimethyl acetal (7.9% yield). This work not only presents a practical method for lignin upgrading but also provides a clear mechanistic roadmap for the future design of selective electrocatalytic processes.
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DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2025
·Vol. 51
·期 11
·14746-14754
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IF 5.6
Boyu Chen,Lijuan Niu,Jianlong Chai,Zhiyuan Bai,Jiayin Zhang,Di Liu,Xuefeng Lu,Yabin Zhu
β-SiC ceramics were prepared by spark plasma sintering at 2200 °C with Y 2 O 3 , MgO , and CeO 2 as sintering additives. Al 2 O 3 was doped based on the above oxides to form a two-component sintering additive to carry out a comparative analysis. The effects of these additives on the microstructure, bulk density, and Vickers hardness of β-SiC were systematically studied. The mechanical properties of the single-component samples were superior to those of the two-component samples. Notably, the single-component sample with Y 2 O 3 , addition exhibited the highest density (98.69 %) and hardness (27.1 GPa), which can be attributed to the appropriate growth of the grains in the single-component sample with Y 2 O 3 . Additionally, the principle of lattice reduction in SiC ceramics was explored using first-principle calculations, further highlighting the advantage of Y 2 O 3 as a single additive for the high-temperature sintering of SiC ceramics.
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高影响因子
Biomaterials Advances
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2022
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IF 0
Dahu Qi,Jin Su,Song Li,Hao Zhu,Lijin Cheng,Shuaibin Hua,Xi Yuan,Jiawei Jiang,Zixing Shu,Yusheng Shi,Jun Xiao
Bioceramics have been used in orthopedic surgery for several years. Magnesium (Mg) is an essential element in bone tissue and plays an important role in bone metabolism. Mg-doped bioceramics has attracted the attention of researchers recently. However, the optimal doping amount of Mg in β-TCP and the immunomodulatory property of Mg-doped β-TCP (Mg-TCP) have not been determined yet. In this study, β-TCP scaffolds doped with different contents of magnesium oxide (0 wt%, 1 wt%, 3 wt%, and 5 wt%) with gyroid structure were printed by digital light processing (DLP) method, and the physicochemical and biological functions were then investigated. Mg-doping improved the physicochemical properties of the β-TCP scaffolds. In vitro experiments confirmed that the doping of Mg in β-TCP scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and angiogenic differentiation of endothelial progenitor cells (EPCs), where the 5Mg-TCP has the optimal properties when using the “one cell type” method. It was also found that all Mg-TCP facilitated the polarization of RAW264.7 cells to the M2 phenotype, especially the 3Mg-TCP. However, 3Mg-TCP displayed the optimal osteogenic and angiogenic potential when using a “multiple cell type” method, which referred to culturing the BMSCs or EPCs in the macrophage-conditioned medium. Finally, the in vivo experiments were conducted and the results confirmed that the 3Mg-TCP scaffolds possessed the satisfying bone defect repair capability both after 6 and 12 weeks of implantation. This study suggests that 3Mg-TCP scaffolds provide the optimal biological performance and thus have the potential for clinical translation.
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PubMed
FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Xin Liu,Peng Wang,Yu-Xiao Zou,Zhi-Gang Luo,Tamer Mahmoud Tamer
Vitamin C (VC) and β -Carotene ( β C) were selected to produce co-encapsulated liposomes using hydrophilic and hydrophobic cavities simultaneously by ethanol injection method. The results of liposomal structure characterized by particle size, polydispersity index, zeta-potential and transmission electron microscope showed that the microstructure of all liposomal samples was spherical without adhesion or break and the size of VC- β C-loaded liposome (L-VC- β C) was bigger than VC-loaded liposome (L-VC) or β C-loaded liposome (L- β C). The encapsulation efficiency (EE) of VC in L-VC- β C was significantly higher than that in L-VC, and the EE of β C in L-VC- β C had no significant change compared with that in L- β C. The free radical scavenging rate of L-VC- β C was significantly higher than that of L- β C, while it had no significant change compared with that of L-VC. In addition, the storage stability of β C in L-VC- β C improved greatly compared with that in L- β C. Furthermore, the zero order model was applied to understand the release kinetics of β C from L- β C and L-VC- β C in the stomach, whereas the Korsmeyr-Peppas model was chosen to describe the release of β C from two types of liposome in small intestine and their release mechanisms were mainly dominated by Fickian diffusion. It was significant to provide a new idea for using hydrophilic and hydrophobic cavities simultaneously in liposomes to design the multicomponent nutrient delivery system.
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PubMed
高影响因子
Frontiers in Pharmacology
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2020
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IF 4.225
Xie Jing,Luo Feng-xian,Shi Chong-ying,Jiang Wei-wei,Qian Ying-yan,Yang Ming-rong,Song Shuang,Dai Tian-yi,Peng Lei,Gao Xiao-yu,Tao Liang,Tian Yang,Sheng Jun
Moringa oleifera Lam. (M. oleifera) is valuable plant distributed in many tropical and subtropical countries. It has a number of medicinal uses and is highly nutritious. M. oleifera has been shown to inhibit tumor cell growth, but this effect has not been demonstrated on prostate cancer cells. In this study, we evaluated the inhibitory effect of M. oleifera alkaloids (MOA) on proliferation and migration of PC3 human prostate cancer cells in vitro and in vivo. Furthermore, we elucidated the mechanism of these effects. The results showed that MOA inhibited proliferation of PC3 cells and induced apoptosis and cell cycle arrest. Furthermore, MOA suppressed PC3 cell migration and inhibited the expression of matrix metalloproteinases (MMP)-9. In addition, MOA significantly downregulated the expression of cyclooxygenase 2 (COX-2), β-catenin, phosphorylated glycogen synthase 3β, and vascular endothelial growth factor, and suppressed production of prostaglandin E2 (PGE2). Furthermore, FH535 (β-catenin inhibitor) and MOA reversed PGE2-induced PC3 cell proliferation and migration, and the effects of MOA and FH535 were not additive. In vivo experiments showed that MOA (150 mg/kg) significantly inhibited growth of xenograft tumors in mice, and significantly reduced the protein expression levels of COX-2 and β-catenin in tumor tissues. These results indicate that MOA inhibits the proliferation and migration, and induces apoptosis and cell cycle arrest of PC3 cells. Additionally, MOA inhibits the proliferation and migration of PC3 cells through suppression of the COX-2 mediated Wnt/β-catenin signaling pathway.
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PubMed
高影响因子
Oxidative Medicine and Cellular Longevity
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2021
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IF 6.543
Yanxia Ma,Yu Liu,Linxin Teng,En Luo,Dekang Liu,Fuqiong Zhou,Kaiyuan Wang,Weiping Chen,Lei Bi
Lung cancer has become the leading cause of cancer-related death worldwide. Oxidative stress plays important roles in the pathogenesis of lung cancer. Many natural products show antioxidative activities in cancer treatment. Zi Shen decoction (ZSD) is a classic prescription for the treatment of lung disease. However, its effect on lung cancer lacks evidence-based efficacy. In this study, we investigated the anticancer effects of ZSD on lung cancer in vivo and in vitro. Our results showed that oral administration of ZSD suppressed the Lewis lung cancer (LLC) growth in a subcutaneous allograft model and promoted necrosis and inflammatory cell infiltration in the tumor tissues. Furthermore, ZSD not only inhibited tumor cell proliferation and migration but also induced cell apoptosis in lung cancer cells. PI3K/AKT signaling is well characterized in response to oxidative stress. The bioinformatics analysis and western blot assays suggested that ZSD decreased the enzyme activity of PI3K and AKT in vivo and in vitro. We also found that the AKT/GSK-3β/β-catenin pathway medicated anticancer effect of ZSD in lung cancer cells. In conclusion, we demonstrate for the first time that ZSD possesses antitumor properties, highlighting its potential use as an alternative strategy or adjuvant treatment for lung cancer therapy.
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2022
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IF 5.307
Yongxi Dong,Mingji Liu,Yongqing Mao,Shuxia Wu,Jinlan Wen,Jun Lu,Yaxin Yang,Shiyang Ruan,Lei Li,Xinyun Liu,Jiquan Zhang,Shanggao Liao,Li Dong
Carnosic acid could disrupt the β-catenin/BCL9 protein-protein interaction and inhibit β-catenin dependent transcription, thereby reduce the incidence of colorectal cancer induced by abnormal activation of Wnt/β-catenin signalling pathway. However, its activity was weak (IC 50 for SW480: 28.2 ± 2.05 μM) and total synthesis was difficult. During the structural simplification of natural products, S0 was revealed to be the basic pharmacophore of carnosic acid. Subsequent structural optimization of S0 led to the discovery of S11 as a possible anticancer agent with prominent proliferation inhibition effect (IC 50 for SW480: 9.56 ± 0.91 μM) and best selectivity index (SI = 3.0) against Wnt hyperactive cancer cells. Futher mechanism investigation through TOP/FOP dual luciferase reporter assay, immunofluorescence, co-immunoprecipitation, microscale thermophoresis, downstream oncoprotein expression and cell apoptosis showed that compound S11 could significantly inhibit the proliferation of SW480 cells via obvioudsly decreasing the nucleus translocation of β-catenin and effectively disrupting β-catenin/BCL9 protein-protein interaction. Additionally, cell migration, molecule docking, in vitro stability and solubility assays were also conducted. Overall, S11 was worthy of in-depth study as a potential inhibitor for the Wnt/β-catenin pathway and its discovery also proved that the structural simplification of natural products was still one of the effective methods to find new lead compounds or candidate drugs.
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PubMed
高影响因子
JOURNAL OF CHEMICAL NEUROANATOMY
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2020
·
IF 2.353
Hongqing Liu,Shiqi Li,Cuizhu Yang,Hui Jia,Ziting Gu,Xing Tu,Sumin Tian,Jing Liu,Guoying Li,Yuxin Ma
The senile plaque formed by β-amyloid (Aβ) deposition in the brain is one of the main pathological features of Alzheimer’s disease (AD), and the c-Jun N-terminal kinase (JNK) signaling pathway plays an important role in the pathogenesis of AD. This study aimed to investigate that D-serine may ameliorate motor and cognitive impairment in Aβ injected mice by inhibiting JNK signaling pathway. Firstly, Kunming mice were injected intrahippocampally with Aβ 1-42 to build AD model. The mice were injected intraperitoneally with saline, D-serine, D-amino acid oxidase (DAAO), and Sodium benzoate (BE) for 10 consecutive days, respectively. Subsequently, the motor and cognitive functions of mice were detected by behavioral tests. The silver staining and immunohistochemical methods were used to detect the distributions of Aβ in the hippocampus of mice. 18 F-2-Fluro-D-deoxy-glucose positron emission tomography/computed tomography ( 18 F-FDG PET/CT) scans were performed to detected glucose metabolism of Aβ 1-42 induced lesions. The expressions of relative JNK factors were detected by immunohistochemistry and Western blot methods. These results showed that Aβ severely impaired the motor and memory abilities of mice. The expressions of glial fibrillary acidic protein (GFAP), tumor necrosis factor (TNF-α), N-methyl-D-aspartate receptor 1 (NMDAR1), phospho-JNK (p-JNK), p-c-Jun and activating transcription factor 2 (ATF2) increased significantly. After D-serine treatment, the abilities of movement and memory of mice were improved, and the clearance rate of Aβ was accelerated. The expressions of GFAP, TNF-α, NMDAR1, p-JNK, p-c-Jun and ATF2 decreased significantly. DAAO and BE were administered to further validate these results. Therefore, this study showed that D-serine could alleviate the cognitive impairment of Aβ 1-42 injected mice by inhibiting JNK signaling pathway. These results provide more evidences for the effect of D-serine on AD and relevant mechanism to treat AD.
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PubMed
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Ying Lin,Ran Huang,Xiuxiu Sun,Xi Yu,Ying Xiao,Ling Wang,Wenzhong Hu,Tian Zhong
In order to develop a biodegradable agent for food preservation, β-cyclodextrin (β-CD) inclusion complexes containing p -Anisaldehyde (PAA) were prepared using co-precipitation method. Physical properties including moisture content, bulk density, hygroscopicity, entrapment efficiency and loading capacity of the inclusion complexes showed considerable results for food applications. SEM and particle size test showed notably difference between the prepared inclusion complexes and the other formulations including the raw β-CD, physical mixture, and the recrystallized β-CD in both shape and size distribution. FTIR , XRD, TGA and DTA were also carried out and confirmed the formation of inclusion complexes and inhibition of the volatility of encapsulated PAA. As a potential preservation agent, inclusion complexes demonstrated a sustained released behavior in phosphate buffer saline (PBS). Comparing with pure PAA, the inclusion complexes presented a better storage stability in low humidity environment, a higher antioxidant activity , and equal MICs against Escherichia coli and Staphylococcus aureus .
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高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Maoyun Li,Xiaoqin Luo,Ruixue Zhu,Kai Zhong,Wenyi Ran,Yanping Wu,Hong Gao
The development of bio-based and active food packaging materials is an effective way to alleviate the growing environmental concern and extend the shelf life of food . This study aimed to prepare a novel bilayer film composed of chitosan (CS) and sodium alginate (SA) incorporated with inclusion complex of dihydromyricetin with hydroxypropyl-β-cyclodextrin (ICDH) by layer-by-layer casting. The results of structural and physical characterization revealed that CS/SA/ICDH films were hydrophilic and had good compatibility, thermal stability, and UV blocking capability, with a smooth and uniform appearance. Compared with CS/SA film, CS/SA/ICDH-10% film exhibited improved tensile strength (45.57 MPa) and elongation at break (22.46%). More importantly, incorporation of ICDH was shown to enhance the antioxidant activity of the bilayer film, as well as the antibacterial activity against Staphylococcus aureus . CS/SA/ICDH-10% film had a superior performance as a food packaging material on preservation of fried meatballs by effectively reducing lipid oxidation and microbial growth. After storage for 7 days, the thiobarbituric acid reactive substance (TBARS) and total plate count (TPC) of fried meatballs packaged with CS/SA/ICDH-10% film were 31.72 μg/g and 3.29 log 10 CFU/g, respectively, which were significantly lower than those of the control group (37.00 μg/g, 5.34 log 10 CFU/g) and plastic film group (41.07 μg/g, 5.97 log 10 CFU/g). Therefore, this study suggested that CS/SA/ICDH film could be used as a promising active packaging material for application in food industry.
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高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2024
·
IF 2.3
Bing Shao,Yang Fu,Bo Li,Siming Huo,Jiayu Du,Xuliang Zhang,Xin Yin,Yanfei Li,Zheng Cao,Miao Song
Faced with infectious bone defects, the development of a thermosensitive hydrogel containing icariin (ICA) represents a promising therapeutic strategy targeting infection control and bone regeneration. In this study, we prepared and evaluated the physicochemical properties, in vitro and in vivo drug release, antimicrobial activity, anti-inflammatory properties, and bone repair effects of ICA/Chitosan/β-Glycerophosphate (ICA/CTS/β-GP) thermosensitive hydrogel. Our findings demonstrate that the ICA/CTS/β-GP thermosensitive hydrogel undergoes a liquid-to-gel transition at body temperature, which is crucial for maintaining local drug release at the defect site. Additionally, the hydrogel exhibited sustained release of ICA over 28 days, showing high antimicrobial activity against Staphylococcus aureus and good biocompatibility in blood compatibility tests. In a canine model of infectious bone defects, the ICA/CTS/β-GP thermosensitive hydrogel showed effective infection control and modulated inflammation, vascular formation, and bone factor expression, while also activating the Wnt/β-catenin signaling pathway. In conclusion, the ICA/CTS/β-GP thermosensitive hydrogel could control infection and repair bone tissue. Its antimicrobial and osteogenic properties provide hope for its clinical application.
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高影响因子
ACS Applied Optical Materials
·
2023
·
IF 0
Jie Zhu,Yang Sun,Meng-Xin Duan,Zheng-Wu Wang,Xin Tan,Zhen Yu,He-Fang Wang
The fast and accurate detection of β-lactamase is a prerequisite of customized treatments and food safety. We present a pretreatment-free dual-excitation three-mode detection of β-lactamase by using ZnAl2O4:Cr3+ etched by ethylenediaminetetraacetic acid disodium (ZAO-EDTA) as a phosphorescent probe and nitrocefin as the signal mediator. The changes in the wavelength overlaps of UV–vis absorbance of nitrocefin upon interactions with β-lactamase and the dual excitation of ZAO-EDTA enable the design of three detection modes: i.e., the enhanced emission when excited at 393 nm, the quenched emission when excited at 533 nm, and the ratio of enhanced excitation of 393 nm and decreased excitation of 533 nm to determine β-lactamase. All three modes have a wide linear range, high sensitivity and selectivity, and fine anti-interference capability. The lowest limit of detection is observed in the ratiometric excitation mode (0.2 U·L–1), and the proposed method can directly detect β-lactamase in bacteria and milk samples without any pretreatments, with the spiked recoveries of 95.3–113% and relative standard deviation < 3.1%. Therefore, the proposed method would be attractive for the detection of β-lactamase in a complex sample, and the ZAO-EDTA probe can be widely used to establish other excitation ratiometric optosensings by changing other signal mediators.
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