JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Li Shuyi,Li Yuguo,Wu Jiang,Gao Yue,Zeng Shiyi,Deng Hao,Hu Xin,Guo Quanyi,Xiao Jingang,Wei Jiawei
Dysregulation of chondrocyte metabolic networks and the physical barrier imposed by the extracellular matrix (ECM) represent primary bottlenecks in precision intervention for osteoarthritis (OA). We engineered an environment-responsive, charge-adaptive biomimetic nanoplatform, IM@PP-R, in which the metabolic modulator RCGD423 was encapsulated within a polycaprolactone (PCL) core via flash nanoprecipitation (FNP), then cloaked with a hybrid shell of neutrophil membranes and ionizable lipids. In the mildly acidic OA microenvironment (pH 6.4–6.8), IM@PP-R undergoes surface charge inversion from negative to positive, converting electrostatic ECM repulsion into attraction to facilitate deep cartilage penetration. Both in vitro and in vivo, IM@PP-R treatment was associated with improved mitochondrial status and attenuated oxidative stress. This process was closely linked to a redirection of the arachidonic acid (ARA) metabolic profile: integrated transcriptomic and metabolomic analyses revealed the downregulation of pro-inflammatory enzymes (ALOX5, mPGES-1) alongside a partial recovery of the IL-1β-suppressed pro-resolving enzyme CYP2J4. These enzymatic shifts corresponded with a transition from PGE2 toward PGA2/PGJ2 accumulation, suggestive of a reprogrammed lipid mediator profile. In a rat ACLT model, IM@PP-R significantly prolonged joint residence time and attenuated osteochondral degeneration. This study presents a translatable strategy for overcoming dense tissue barriers to achieve intracellular metabolic reprogramming in OA. Graphical
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Guan Xinhui,Zhang Yuying,Ma Xiaoqiao,Wang Haiyan,Qiao Xiaoqiang,Gu Xiaofei
Comprehensive characterization of exosomal phospholipids is essential for understanding cancer-associated metabolic alterations and discovering potential biomarkers, but remains challenging due to their extensive structural diversity and the limited selectivity and coverage of conventional liquid chromatography-tandem mass spectrometry (LC-MS/MS) approaches. In this study, an ionic liquid-functionalized styrene-maleic anhydride copolymer brush stationary phase (Sil-BHHICl) was developed and integrated into an offline two-dimensional (2D) LC-MS/MS platform for comprehensive exosomal phospholipid profiling. The Sil-BHHICl stationary phase exhibited a hydrophilic interaction/reversed-phase mixed-mode retention mechanism and provided enhanced selectivity toward phospholipids, enabling efficient separation at both the phospholipid-class and molecular-species levels. The developed analytical platform enabled comprehensive profiling of plasma-derived exosomal phospholipids, resulting in the identification of 274 molecular species belonging to 10 phospholipid classes. Lipidomic analysis of plasma exosomes from breast cancer patients and healthy controls revealed distinct phospholipid alterations associated with breast cancer and identified five candidate biomarkers, among which LysoPC-O (16:0) exhibited the strongest discriminatory ability. Pathway enrichment analysis further indicated that the altered phospholipids were predominantly associated with glycerophospholipid metabolism, suggesting extensive lipid metabolic remodeling in breast cancer. Overall, this study establishes a mixed-mode chromatographic platform for high-coverage exosomal phospholipid profiling and demonstrates its potential for lipidomics-based biomarker discovery and investigation of disease-associated metabolic alterations. Graphical abstract
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高影响因子
CHEMICAL PAPERS
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2026
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IF 2.7
Zhong Xiangyan,He Xinye,Long Ruiqing,Qi Linfeng,Zhao Qiang
Efficient utilization of long-wavelength visible light remains a key challenge in developing metal-organic-framework-based photocatalysts for solar-driven pollutant removal. To address this issue, carbon quantum dot (CQD)-modified UiO-66-NH 2 hybrid materials with different nominal CQD loadings were constructed through induced loading and secondary thermal fixation to combine spectral conversion with interfacial photophysical regulation. Structural and textural characterizations showed that CQD incorporation preserved the crystalline and porous framework of UiO-66-NH 2 while modifying its surface composition, pore characteristics, and optical response. The CQDs displayed visible-to-ultraviolet anti-Stokes emission centered at approximately 340 nm under 600 nm excitation. CQD incorporation also enhanced visible-light absorption and induced photoluminescence quenching consistent with interfacial photophysical coupling. Transient-current measurements showed reproducible light-switching responses, while EIS revealed a lower impedance response for CU-3 under illumination; together, these results are consistent with facilitated interfacial charge migration. Under visible-light irradiation in the presence of FeCl 3 as an electron scavenger, CU-3, containing 3 wt% CQDs, achieved a background-corrected net methyl orange removal of 67% within 90 min. Its apparent rate constant was 1.203 × 10 − 2 min − 1 , approximately 6.6 times that of pristine UiO-66-NH 2 . Tert-butanol inhibition and DMPO spin-trapping ESR measurements jointly supported the involvement of hydroxyl radicals, with CU-3 exhibiting a stronger light-induced DMPO–·OH signal than pristine UiO-66-NH 2 . The effect of EDTA-2Na could not be assigned exclusively to photogenerated holes because of its complexation with Fe 3+ . These findings demonstrate the importance of optimizing CQD loading and suggest that spectral conversion and interfacial photophysical coupling contribute to the enhanced apparent visible-light activity.
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高影响因子
Nature Microbiology
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2026
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IF 18.7
Wu Xiaolei,Wang Teng,Jia Chenghao,Zhou Haiyang,Li Yan,Baker Stephen,Yue Min
Understanding the genetic factors facilitating emergence of infectious diseases is critical, however, mechanisms underlying expansion of pathogenic bacterial variants remain unclear. Here we performed a large-scale genomic analysis of 44,597 Salmonella Typhimurium genomes and observe that sequential gene loss in monophasic Salmonella Typhimurium (mSTM) ST34 explains its clonal expansion as an increasingly prevalent zoonotic lineage. Functional and in vivo competition experiments show that a frameshift mutation in dinB , a polymerase for translesion DNA synthesis, leads to transcriptional changes affecting flagellin gene expression and subsequent loss of the flagellin-encoding fljB , altering the requirements for gut infection. Temporal evolutionary modelling supports a role for gene loss events in a specific chronological order for mSTM ST34 expansion. Our findings reveal a stepwise pathoadaptation model underpinning clonal global spread, providing mechanistic insights relevant to forecasting future pandemics.
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高影响因子
Stem Cell Research & Therapy
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2026
·Vol. 17
·期 1
·312
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IF 7.8
Shao Qi,Shi Jianhong,Lu Jin,Tian Yuanye
Background Craniofacial bone defects represent significant clinical challenges resulting from diverse etiological factors. Despite advancements in biomaterials science, autologous tissue transplantation remains the gold standard for craniofacial reconstruction due to the limitations of existing materials in replicating the complexity and functionality of native tissues. Objective This study aimed to investigate the efficacy of combining 3D-printed nano-hydroxyapatite (n-HA) scaffolds with concentrated growth factor-enriched cell sheets (CGF-BMSCs-CS) for bone defect repair, exploring their synergistic effects on osteogenesis and tissue regeneration. Methods Bone marrow mesenchymal stem cells (BMSCs) were utilized to fabricate CGF-enriched cell sheets. 3D-printed porous n-HA scaffolds (5 mm diameter, 1 mm thickness) were prepared and wrapped with CGF-BMSCs-CS to form composite scaffolds. Four experimental groups were established: control, n-HA alone, CGF-BMSCs-CS alone, and the composite n-HA/CGF-BMSCs-CS. Osteogenic potential was evaluated through alkaline phosphatase (ALP) activity assays and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of osteogenesis-related gene expression. Sprague-Dawley rats were randomly assigned to these groups for a cranial defect model. Micro-computed tomography (µCT) was performed at 6 and 12 weeks post-surgery to assess bone regeneration metrics, including bone volume fraction (BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th). Results Scanning electron microscopy (SEM) confirmed the n-HA scaffold’s uniform porosity and structural integrity. The CGF-BMSCs-CS exhibited optimal thickness and viscosity for tissue engineering applications. The composite group demonstrated significantly elevated ALP activity and upregulated expression of osteogenesis-related genes, indicating enhanced osteogenic capacity. µCT analysis revealed superior bone formation and osseointegration in the composite scaffold group compared to other groups. This was further supported by significantly higher bone mineral density (BMD) values at both time points ( p < 0.05). Histological evaluation via hematoxylin and eosin (H&E) staining showed increased osteocyte activity, neovascularization, and trabecular bone formation in the composite group. Conclusions We innovatively integrated 3D printed n-HA scaffolds with CGF BMSCs CS, which combines mechanical stability with enhanced osteogenic potential for craniofacial bone defect repair, promoting bone healing. This innovative strategy provides promising prospects for advancing bone tissue engineering and regenerative medicine. Graphical
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高影响因子
Nature Communications
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2026
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IF 18.1
Nan Youbo,Li Xiang,Wang Xiutong,Du Yan,Hu Jiaxin,Han Rongbing,Chen Baodong,Wang Zhong Lin,Wei Di
Electrical double layers (EDLs) at solid-liquid interfaces mediate charge separation and ion transport, but their dynamic use for both energy harvesting and sensing in open water remains limited. Here we show that wave-driven multi-interface electrical double layers in a tubular dielectric architecture can couple power generation with environmental perception. Periodic water motion assembles and disrupts coupled inner and outer interfacial layers, producing a charge-relay and parallel-release process that enhances charge transfer and short-circuit current to levels sufficient for low-power sensing. The same ion motion that generates electricity also encodes hydrodynamic and spatial perturbations as current signatures. Inspired by weakly electric fish, we organize these signatures using a main-auxiliary electrode network that amplifies directional responses and enables coating monitoring, wave-state recognition and two-dimensional localization. This work establishes dynamic multi-interface electrical double layers as a physical platform for self-powered hydrospheric sensing and spatially resolved environmental perception.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Yuewen Xue,Lina Sun,Qinghua Tan,Tongtong Cui,Wanzhu Hao,Xia Luo,Ningyi Xian,Xijing He,Leilei Zhang,Ting Zhang
Polyetherketoneketone (PEKK) has attracted interest for load-bearing orthopedic implants due to its favorable elastic modulus and radiolucency; however, its biological inertness and weak interfacial bonding limit its clinical application. Here, we developed a dual-reinforced PEKK composite by incorporating hydroxyapatite (HA) particles and silicon nitride (SN) nanowires to improve its mechanical, tribological, antibacterial, and osteogenic properties. The ternary PEKK/HA/SN composite (PSH) exhibited tensile, compressive, and flexural strengths of 112.23, 241.36, and 201.48 MPa, increased from 23.04, 92.34, and 60.23 MPa for pure PEKK, respectively. Under dry sliding conditions, PSH showed a friction coefficient of 0.34 and a wear rate of 0.69 × 10 − 5 mm 3 /N·m, corresponding to reductions of 30.6% and 24.2% compared to PEKK, respectively. In bovine serum, the friction coefficient and wear rate further decreased to 0.23 and 0.63 × 10 − 5 mm 3 /N·m, with reductions of 45.2% and 25.9%, respectively. Furthermore, PSH exhibited antibacterial activity, with inhibition rates of 66.81% against E. coli and 75.25% against S. aureus , and reduced S. aureus biofilm biomass to 48.77 ± 6.51% of pristine PEKK. Biological assays showed improved osteoblast proliferation, osteogenic differentiation, and peri-implant bone formation in vivo. These findings suggest that the combined incorporation of HA and SN nanowires may provide a feasible strategy for improving the mechanical strength, tribological stability, antibacterial activity, and osteogenic performance of PEKK-based composites.
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PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2026
·
IF 15
Chang Xiong,Liubing Chen,Zilong Rao,Jinyan Guo,Jiaming Ji,Zhenyu Yang,Jinyu Liu,Chengpeng Han,Ziqing Hei,Ying Bai,Weifeng Yao
Eosinophils have recently been recognized as hepatoprotective immune cells; however, cell-based therapies still face significant challenges. Apoptotic extracellular vesicles (ApoEVs) serve as critical cell-free alternatives capable of promoting tissue repair, yet exhibit marked functional heterogeneity dependent on their cell source. The therapeutic potential and mechanisms of eosinophil-derived ApoEVs (Eos-ApoEVs) in steatotic liver ischemia/reperfusion (I/R) injury remain elusive. Herein, treatment with Eos-ApoEVs effectively reversed oxygen–glucose deprivation/reperfusion (OGD/R)-induced cellular dysfunction in hepatocytes and liver sinusoidal endothelial cells (LSECs). Moreover, Eos-ApoEVs exerted potent immunomodulatory effects. Mechanistically, Eos-ApoEVs are enriched with functional mitochondria that fuse with damaged mitochondria in recipient cells, thereby reprogramming energy metabolism to promote hepatocyte proliferation, restore LSEC migration and tube-forming capacity, and facilitate anti-inflammatory macrophage polarization. To develop a clinically translatable therapeutic approach, we constructed a decellularized amniotic matrix (dAM)-based hydrogel patch (ApoEVs@dAM/HA-DA), which enables sustained release of ApoEVs and exhibits high biocompatibility. In a mouse model of steatotic liver I/R injury, transplantation of ApoEVs@dAM/HA-DA onto the liver surface significantly increased anti-inflammatory macrophage polarization, attenuated inflammation, promoted hepatocyte proliferation and enhanced angiogenesis, thereby accelerating liver repair. Thus, surface transplantation of ApoEVs@dAM/HA-DA provides a clinically viable and potent therapeutic strategy for steatotic liver I/R injury.
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PubMed
高影响因子
IONICS
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2026
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IF 3.4
Du Yongli,Cao Yongfan,Cao Zhenzhu,Wang Liying
Composite solid electrolytes (CSEs) have both high safety and long cycle life, and are the key materials for the next-generation lithium batteries. However, due to low ionic conductivity and poor interfacial compatibility, their practical application in high-energy-density lithium metal batteries is limited. This work introduces one-dimensional (1D) halloysite nano tube (HNT) into a Polyvinylidene fluoride (PVDF)/ lithium bis(trifluoromethanesulfonyl)imide (LiTFSI)/ Li 6.85 La 2.95 Yb 0.05 Zr 1.85 Ta 0.15 O 12 (LLYZTO) CSE (PLLH- x ), and investigates its effect on the structure, mechanical and electrochemical properties. HNTs nanotubes with external negative and internal positive electrical polarity, can construct continuous lithium ions (Li⁺) transport pathways between LLYZTO particles and delay the diffusion of TFSI - anion. The obtained PLLH-7 electrolyte has an ionic conductivity of 8.52 × 10 -4 S cm -1 , a Li⁺ transference number of 0.80 and a mechanical strength of 12.3 MPa. The Li symmetric cells assembled based on it exhibit long cycle stability for 2400 h and realize effective inhibition of lithium dendrites. The Li/PLLH-7/LiFePO 4 (LFP) battery still has a discharge specific capacity of 131.6 mA h g -1 after 350 cycles at 0.2 C, with the Coulombic efficiency of 95.4%, showing excellent cycle stability. The Li/PLLH-7/LiNi 0.8 Co 0.1 Mn 0.1 O 2 (NCM811) battery also exhibits excellent high-voltage and high-rate tolerance, with the specific capacity maintaining at 124.1 mA h g -1 after 500 cycles at 1 C. This study provides an effective method to improve the ionic conductivity, mechanical and electrochemical property of PVDF composite electrolyte.
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高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2026
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IF 1.7
Guo Yuanyuan,Wang Ying,Shan Haiwei,Zhang Lieyu,Wang Hongqiang
The treatment of uranium-containing wastewater faces major challenges, including complex compositions, strong interference from coexisting ions, poor selectivity of conventional methods, and rapid blockage of electrode active sites. Here, we utilize expired flour as a low-cost biomass carbon/nitrogen source and copper oxalate as a copper precursor to prepare a biomass-derived carbon-supported copper single-atom catalyst (Cu-SAs/BC). This catalyst is then composited with g-C 3 N 4 at an optimal mass ratio of 2:3 and loaded onto carbon felt to construct a Cu-SAs/BC-g-C 3 N 4 photoelectrocatalytic electrode. The electrode achieves a uranium removal efficiency of 99.4% within 5 min under optimal conditions (total loading 13 mg, pH 4–6), and exhibits rapid removal across a wide initial concentration range of 5–100 mg/L. The distribution coefficient for U(VI) reaches 6.5 × 10 4 mL/g, significantly surpassing those of coexisting interfering ions (Al, Zn, Fe, Mn, Ca). Complete uranium removal from actual uranium mining seepage water is achieved within 20 min. Mechanistic studies confirm that phosphate groups on the electrode surface specifically chelate and coordinate with UO 2 2+ enabling rapid and targeted enrichment of uranyl ions. The UO 2 2+ ions then migrate to Cu-N x active sites, accept electrons, and undergo reduction to form U(Ⅳ), producing UO 2 precipitates that automatically detach under square-wave potential for efficient separation. This study provides a low-cost, sustainable biomass-derived single-atom catalytic material and a technological pathway for uranium recovery from complex radioactive wastewater.
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高影响因子
CLINICAL RHEUMATOLOGY
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2026
·
IF 2.7
Li Zhiqiang,Cao Liang,Wang Jiajia,Gao Xia,Suo Fengtai,Luo Fengshou,Xu Wentao,Wang Zhenzhong,Zhang Xinzhuang,Xiao Wei
Objective This study aimed to evaluate the therapeutic efficacy of Compound Nanxing Zhitong Plaster (CNZP) against rheumatoid arthritis (RA) and to investigate its underlying mechanism through an integrated approach combining network pharmacology and metabolomics. Methods A collagen-induced arthritis (CIA) rat model was established to assess the anti-RA effects of CNZP. A comprehensive strategy, incorporating network pharmacology (based on previously reported chemical constituents), molecular docking, serum biochemical assays, histopathological examination, immunohistochemistry, and Western blotting, was employed to elucidate its therapeutic actions, associated pathways, and molecular targets. Results Network pharmacology identified 53 common targets between CNZP and RA, with IL1β, TLR4, and STAT3 as the top three hub genes. GO and KEGG enrichment analyses revealed that the therapeutic effects of CNZP are primarily mediated through inflammatory response, endopeptidase activity, and signaling pathways such as TNF, IL-17, and Toll-like receptor signaling. Quercetin, imperatorin, and isoimperatorin were identified as the core components. Molecular docking confirmed favorable binding affinities of these three components to IL1β, TLR4, and STAT3. Metabolomics analysis revealed eight potential biomarkers, among which six were significantly modulated by CNZP. These metabolites were primarily involved in arginine and proline metabolism, histidine metabolism, and glycerophospholipid metabolism pathways. Integrated analysis of metabolomics and network pharmacology highlighted the arginine and proline metabolism and arginine biosynthesis pathways as the common mechanisms. l -Arginine was pinpointed as the key differential metabolite, with nitric oxide synthase 1 (NOS1) as its associated regulatory enzyme. CNZP treatment is associated with reduced activation of the JAK1/STAT1 pathway and downregulation of NOS1, suggesting a potential involvement of this axis in the therapeutic effect of CNZP against RA. Conclusion CNZP exhibits significant therapeutic effects on RA, potentially by downregulating NOS1 expression and subsequently correcting the metabolic imbalance of l -arginine.
Key Points • CNZP demonstrated significant therapeutic efficacy in a CIA rat model; serum metabolomics revealed the associated differential metabolites and key metabolic pathways involved in its action. • Network pharmacology and molecular docking revealed that CNZP exerts multi-target effects on RA by modulating TNF, IL-17, and Toll-like receptor signaling pathways via its core components quercetin, imperatorin, and isoimperatorin. • Integrated analysis suggests that CNZP treatment is associated with downregulation of NOS1 and correction of l-arginine metabolic imbalance, implicating the JAK1/STAT1 pathway in its anti-RA effect. • This preclinical study provides mechanistic evidence for the anti-RA effect of CNZP.
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高影响因子
CELL BIOCHEMISTRY AND BIOPHYSICS
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2026
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IF 3
Chen Zhi,Liang Dan,Liu Zhai,Ren Yu,Wang Zhimei,Wan Jun,Tang Yi,Zhou Yibo
Nasopharyngeal carcinoma (NPC) remains a major clinical challenge, particularly at advanced stages. RNA-binding proteins (RBPs) are important post-transcriptional regulators of tumorigenesis, but their roles in NPC remain poorly understood. This study investigated whether RNA-binding motif protein 4 (RBM4) stabilizes homeobox B13 ( HOXB13 ) mRNA and thereby activates oncogenic signaling to promote NPC progression. Bioinformatics analysis identified differentially expressed genes in NPC. ENCORI was used to predict potential RNA-binding proteins (RBPs) targeting the candidate gene, and the predicted interaction was validated by RNA immunoprecipitation. Functional effects of HOXB13 and RBM4 in NPC cells were examined using colony formation and Transwell assays, along with modulating RBM4, HOXB13, nuclear factor kappa B (NF-κB) p65, and β-catenin expression. In vivo, effects of the RBM4/HOXB13 axis on NPC tumor growth and metastasis were evaluated using murine xenograft and lung metastasis models. HOXB13 was among the most strongly upregulated genes in NPC, and RBM4 was predicted to be a candidate RBP targeting HOXB13 mRNA. RBM4 was also upregulated in NPC models and increased HOXB13 mRNA stability. RBM4 silencing suppressed NPC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while increasing apoptosis and reducing HOXB13 expression and NF-κB and Wnt/β-catenin signaling. Overexpression of HOXB13, NF-κB p65, or β-catenin partially reversed these effects. In vivo, RBM4 silencing inhibited xenograft growth and experimental lung metastasis, and these effects were partially reversed by HOXB13 overexpression. RBM4 acts as a pro-tumorigenic RBP in NPC by stabilizing HOXB13 mRNA and activating the NF-κB and Wnt/β-catenin pathways. The RBM4/HOXB13 axis therefore warrants further investigation as a potential molecular target in NPC.
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高影响因子
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