ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Song Xinyi,He Zhaoyuan,Tan Siyuan,Cong Siyu,Lu Wuli,Mai Zedeng,Jiang Mingsheng,Wen Yu,Liu Cheng,Chen Hailan
Deoxynivalenol (DON), a highly stable and globally prevalent mycotoxin, has become a critical food safety concern due to its widespread contamination in animal feed, particularly in corn, wheat, and other cereal-based ingredients. As one of the most frequently detected mycotoxins in feedstuffs, DON contamination not only causes significant economic losses in livestock production but also poses severe health risks to animals, including vomiting, immunosuppression, and growth retardation, which may ultimately affect human health through the food chain. Existing detection methods for DON are often limited by time-consuming procedures or analytical complexity. Here, we report a novel surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) for rapid, sensitive, and specific detection of DON residues in animal feeds. The biosensor employs core-shell Au@4-MBA@Ag nanoparticles functionalized with 4-mercaptobenzoic acid (4-MBA) as a Raman reporter and DON-specific antibodies for target recognition. After systematic optimization, the SERS-LFIA platform achieved both qualitative and quantitative detection within 16 min, with an exceptionally low limit of detection (LOD) of 0.01 µg/kg. The assay demonstrated excellent specificity, showing no cross-reactivity with other major mycotoxins, including aflatoxin B 1 , zearalenone, and ochratoxin A. Remarkable reproducibility was evidenced by intra- and inter-batch coefficients of variation (CV) of 3.51% and 2.19%, respectively. The test strips maintained stability for at least 3 months at 4 ± 0.5 °C and for 2 months at 25 ± 0.5 °C, making them particularly suitable for routine monitoring in feed mills and farms. This innovative SERS-LFIA system represents a significant advancement in mycotoxin detection technology, offering a robust, field-deployable solution for monitoring DON contamination in animal feed, which is crucial for ensuring feed safety and animal health. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 11
·1-10
·
IF 5.3
Wang Qian,Hu Rongjing,Zhao Huifang,He Xiaojing,Zhang Ruiping,Dong Yongqiang
Coin-metal@semiconductor nanohybrids have attracted extensive attention in surface-enhanced Raman scattering (SERS) detection, aiming to achieve the synergistic effect of electromagnetic enhancement mechanism (EM) and chemical enhancement mechanism (CM). In this work, positively charged thin-layered molybdenum disulfide nanosheets (MoS 2 NSs) and negatively charged gold nanoparticles (AuNPs) were synthesized, which were further fabricated into MoS 2 NSs@AuNPs via electrostatic interactions and Au–S bonding. The approach is quite rapid, cost-effective, and environmentally friendly, and the resulting MoS 2 NSs@AuNPs exhibited an outstanding SERS enhancement factor of up to 2.33 × 10 8 for crystal violet due to the synergistic effect of EM and CM. Furthermore, the fabricated SERS substrate demonstrated long-term stability, maintaining about 80% of SERS activity even after storage for two months, and excellent reproducibility, with a relative standard deviation of 5.20%. Remarkably, this SERS-active nanohybrid enabled rapid and highly sensitive detection of thiram residues in apple juice, achieving satisfactory recoveries of 82.6–110.7% and an impressively low limit of quantification of 50 µg/L. This work not only presents a novel strategy for designing high-performance SERS nanomaterials but also highlights their promising applications in food safety monitoring and environmental analysis. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Gao Shengyong,Zhu Yibo,Shi Ke,Liu Peng,Zhang Yiming,Hu Junping,Wen Zhenhai,Wang Lina,Tan Wen,Wang Lianzhou,Luo Bin,Zhou Jisheng
Atomically dispersed metals offer advantages in guiding sodium deposition, yet the effects of single atoms on their surrounding structures and the precise tuning of coordination-governed single-atom activity remain underexplored. Herein, carbon nanofiber films with tin single atoms anchored via a dynamic coordination mode (shifting from coordination with three nitrogen atoms and one oxygen atom to coordination with one nitrogen atom and three oxygen atoms) are developed to address these challenges. The tin atoms not only enhance the sodium-ion adsorption activity of their directly coordinated nitrogen and oxygen atoms but also activate remote carbon atoms. The activation capability is strongly dependent on coordination environment, with tin atoms coordinated to more nitrogen atoms exhibiting higher activity. As a result, the optimized tin–carbon host enables uniform sodium deposition and complete stripping, allowing symmetric cells to cycle stably for 1200 h at 100 mA cm −2 and 100 mAh cm −2 with 100% depth of discharge. Anode-free full cells pairing the tin-carbon host with a sodium vanadate phosphate cathode achieve 94% capacity retention after 700 cycles at 10 C (6 min).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Sui Xiaoyu,Zhao Jiwu,Zhang Pu,Wang Ying,Zheng Hangbin,Zeng Haihua,Wang Pengzhao,Jia Yanyan,Wen Na,Ding Zhengxin,Zhang Zizhong,Dai Sheng,Xu Chao,Yuan Rusheng,Dai Wenxin,Fu Xianzhi,Long Jinlin
The non-oxidative dehydrogenation of light alkanes to alkenes is thermodynamically limited by the trade-off between the cleavage of C-H and C-C bonds. Unlocking the thermodynamic bottleneck with photocatalysis is prone to eliminate undesirable side reactions such as deep dehydrogenation, cracking, isomerization, and polymerization. Herein, we show the photocatalytic non-oxidative dehydrogenation of ethane to ethene and hydrogen at ambient conditions, which is enabled by grafting of Ni single atoms to modulate the surface electronic structure of Pd nanoparticles photo-deposited on the surface of anatase TiO 2 nanoparticles, modifying the ethane dehydrogenation pathway. A high rate of 8.2 ± 0.2 mmol·g −1 ·h −1 for the stoichiometric conversion of ethane to ethene and hydrogen is achieved with a 100% ethene selectivity in a flow reactor under solar light irradiation. The apparent quantum efficiency reaches ~22.3% at 350 nm by using the optimal T-Ni 0.6 Pd 0.24 photocatalyst. Solar-driven non-oxidative alkane dehydrogenation offers a route to light alkenes with high performance, and selectivity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2025
·Vol. 41
·期 10
·1-17
·
IF 4.2
Li Jiaoqing,Huang Guohao,Zeng Jie,Wang Xiaodong,Wang Tan,Li Lin
The carbamate pesticide carbaryl poses significant ecological and health risks due to its widespread misuse and improper long-term disposal. In this study, we engineered a novel bacterial carbaryl-degrading esterase to enhance enzymatic activity, thermostability, and carbaryl degradation through semi-rational design and whole-cell biocatalysis strategies. An esterase, designated Est03320, was identified from Bacillus velezensis MB01B. Phylogenetic analysis revealed it resides in a distinct clade. However, it contains a catalytic domain similar to those of the well-characterized carbaryl-hydrolyzing esterase CarH and the chlorpyrifos-degrading esterase MPH. Est03320 was expressed and purified from Escherichia coli BL21(DE3) cells, demonstrating high hydrolytic activity against short-chain p -nitrophenyl esters and carbaryl. The enzyme exhibits an optimum pH of 6.5 and an optimal temperature of 25 °C, with a K m of 139.70 µM and a V max of 29.47 µM/min. The mutant Est03320-L230G, engineered through a combined semi-rational design and site-directed mutagenesis approach, exhibited a 68.7% increase in enzymatic activity toward the substrate p -nitrophenyl acetate, an extended half-life at 25 °C from 13.6 h to 40.6 h, and a 96.5% enhancement in carbaryl degradation efficiency. To construct a whole-cell biocatalyst, this mutant esterase was further fused with the autotransporter EhaA and displayed on the surface of engineered Pseudomonas sp. MB04R-14-03320-L230G-EhaA cells. The resulting biocatalyst showed high efficiency in degrading and mineralizing carbaryl, retaining over 83% of its activity after five consecutive degradation cycles. This integrated approach significantly enhances both enzymatic performance and reusability, offering a sustainable and effective approach for mitigating carbaryl pollution and advancing environmental protection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Reproductive Biology and Endocrinology
·
2025
·Vol. 23
·期 1
·1-15
·
IF 4.7
Sun Lu,Huang Jiayu,Wang Xuezi,Huang Peng,Dong Baolin,Liang Zehang,Wu Jiahong,Wang Jiancheng
Background Testosterone deficiency (TD) occurs most frequently in older men and can cause many health problems. Testosterone replacement therapy (TRT) is widely used to treat TD, but this regimen can lead to a series of side effects. Stem cell therapy has been wildly studied in vitro. However, due to the multidirectional differentiation potential and heterogeneity of stem cells, it is difficult to achieve the good efficiency and reproducibility in basic research and clinical applications. This study aims to identify a new strategy for the treatment of TD. Methods Bone marrow-derived mesenchymal stromal cells (BMSCs) were enucleated by Ficoll density gradient centrifugation. The organelles and cellular functions of enucleated BMSCs were analyzed by immunofluorescence staining and flow cytometry. Extracellular vesicles (EVs) were isolated by ultracentrifugation and characterized. For the animal studies, enucleated BMSCs were labelled with Mitotracker and injected into ethane dimethanesulfone (EDS)-treated rats. Testosterone production and spermatogenesis were detected at different time points through various tests. To determine the mechanism of efferocytosis, we analysed the number of macrophages by immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR). Results The injection of enucleated BMSCs (Cargocytes) into the testes of EDS-treated rats restored the levels of serum testosterone, increased the number of Leydig cells (LCs), and improved spermatogenesis. We found that enucleated BMSCs underwent apoptosis earlier than BMSCs did. Subsequently, testicular interstitial macrophages phagocytosed apoptotic enucleated BMSCs through efferocytosis. Efferocytosis promoted macrophage polarization from the M1 to the M2 phenotype, reduced the expression of proinflammatory cytokines, and decreased the levels of inflammation and oxidative stress. Conclusions In summary, this study pioneered the application of stromal cell enucleation technology to repair tissue damage in the reproductive system, explored the potential of cell burial in the treatment of reproductive system diseases and provided a new approach for the clinical treatment of male infertility.
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Zhou Xing,Chang Chia-Yu,Yu Dongfang,Zhang Kai,Li Zhi,Jiang Shi-Kai,Zhu Yizhou,Xia Yongyao,Hwang Bing Joe,Wang Yonggang
The application of high-voltage positive electrode materials in sulfide all-solid-state lithium batteries is hindered by the limited oxidation potential of sulfide-based solid-state electrolytes (SSEs). Consequently, surface coating on positive electrode materials is widely applied to alleviate detrimental interfacial reactions. However, most coating layers also react with sulfide-based SSEs, generating electronic conductors and causing gradual interface degradation and capacity fading. To address this, we propose a Li 2 ZrF 6 coating layer on LiCoO 2 , which exhibits minimal reaction with SSEs, and its decomposition products are electron-conductive-free. Furthermore, this coating layer also efficiently mitigates the layered-to-spinel/rock-salt surface structural transformation in LiCoO 2 . As a result, the In-Li|Li 6 PS 5 Cl | Li 2 ZrF 6 -LiCoO 2 all-solid-state cell demonstrates an initial areal capacity of 5.2 mAh cm −2 and a capacity retention of 80.5% after 1500 cycles at 70 mA/g with high LiCoO 2 areal mass loading (30.19 mg cm −2 ) and a cut-off voltage of 3.9 V (corresponding to potential of 4.5 V versus Li + /Li), at 25 °C.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2025
·Vol. 32
·期 6
·1-13
·
IF 2.8
Yin Changjie,Wang Boyu,Li Huimin,Yin Dezhong,Zhang Qiuyu
A new well controllable vinyl silane grafted styrene butadiene rubber (SBR) was firstly prepared by atom transfer radical polymerization (ATRP) in latex. The brominated SBR (SBR-Br) was prepared by the reaction between the SBR and N-bromosuccinimide (NBS), then vinyl silane grafted SBR with well-controlled branch chain was prepared by the ATRP initiated by SBR-Br. The best grafting condition was obtained, that is the reaction temperature and time were 70 °C and 12 h, the mass ratio of monomer: CuBr: dNbpy (4,4'-Dinonyl-2,2'-bipyridine) was 3: 0.02: 0.01. The SBR-Br and grafted SBR were characterized by ATR-FTIR and 1 H-NMR. The reaction kinetics study proved that the process of grafting reaction had active/controllable characteristics. The grafted yield of SBR could reach to 11.62%. The significant phase separation of grafted SBR was observed, and it also had a typical core–shell structure. The tensile strength of vinyltrimethylsilane grafted SBR were increased by 2.66 times than that of raw SBR.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-12
·
IF 5.3
Chen Yafei,Zhang Xuhui,Huang Wanjin,Jin Zhenhuan,Wei Xiaoping,Li Jianping
Atrazine (ATZ) is used extensively, resulting in residues in food and the environment, posing a serious threat to human health. Herein, Cd 0.5 Zn 0.5 S/Ti 3 C 2 photoelectric material was synthesized and immobilized on a FTO electrode as a photoanode. A photoelectrochemical (PEC) aptamer sensor was constructed for the highly sensitive and selective determination of ATZ based on signal amplification via cascade catalysis of CRISPR/Cas12a and G-quadruplex/hemin DNAzyme (G4/hemin DNAzyme). G4/hemin DNAzyme catalyses the oxidation reaction between H 2 O 2 and dopamine (DA) to form polydopamine (PDA) deposit. This process, in turn, inhibits the photocurrent at the photoanode, leading to a decrease in photocurrent. Concurrently, the depletion of DA as an electron donor for the PEC reaction at the photoelectrode further contributes to the decrease in photocurrent. ATZ can hybridize with ATZ aptamer (Apt) in Apt/cDNA to release activation strand (cDNA), which activates the activity of CRISPR/Cas12a and triggers cleavage of G4, causing the cleaving of G4/hemin DNAzyme immobilized on the electrode surface. This process leads to a decrease of G4/hemin DNAzymes amount on the electrode, consequently reducing both the PDA generation and the DA consumption. As a result, the photocurrent is restored. The cascade catalysis of CRISPR/Cas12a and G4/hemin DNAzyme has been demonstrated to result in photocurrent amplification. The photocurrent change was linear with the logarithmic value of ATZ concentration in the range 1.00 × 10 –12 to 1.00 × 10 –5 mol/L. The limit of detection was 3.47 × 10 –13 mol/L. The sensor has been successfully applied to the determination of trace ATZ in environmental and food samples. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOM
·
2025
·
IF 2.3
Zhao Yingqing,Deng Yifan,Liu Jieqiang,Li Min,Li Ying
Sodium-ion batteries (SIBs) are usually considered as a promising alternative to lithium-ion batteries due to the abundant resources of sodium. Antimony has been widely used for SIBs anodes because of its high capacity, which often suffers the issue of unstable structure, leading to a poor performance and unstable solid electrolyte interface (SEI). In order to improve the stability of anodes, the electrochemical reduction of solid antimony sulfide/carbon nanotubes (Sb 2 S 3 /CNTs) to a antimony/carbon nanotubes (Sb/CNTs) composite used as an anode for SIBs using deep eutectic solvent is reported for the first time. The electrolytic Sb particles will be wrapped by the CNTs after the electrochemical desulfurization approach, resulting in an improvement of the stability of the electrolytic products used for anodes. The electrolytic Sb/0.5CNTs anode exhibits a high specific capacity of 612.3 mAh g −1 after 200 cycles at a current density of 0.1 A g −1 , and a high specific capacity of 480.9 mAh g −1 after 150 cycles at a current density of 0.5 A g −1 . The results demonstrate the feasibility of the direct electrochemical desulfurization strategy from solid sulfides and provide a novel route for producing SIBs anodes.
✓ 已验证
产品关联
DOI
高影响因子
ONCOGENE
·
2025
·
IF 7.3
Wei Xiaosong,Wang Beibei,Yang Yang,Fang Zhiwei,Yi Chengzhi,Zhang Liuhui,Fan Xin,Tang Dongdong,Zhang Lirong,Yang Xiaoming,Song Dongkui
Zinc finger protein 24 (ZNF24) is a conserved multifunctional transcription factor associated with tumorigenesis, but its function in bladder carcinogenesis remains unclear. Herein, the expression of ZNF24 was decreased in bladder cancer (BC) cells and tissues, and patients with higher expression of ZNF24 had a better prognosis. Doxycycline-induced overexpression and knockdown of ZNF24 identified its anti-proliferative and anti-metastasis role in BC in vitro and in vivo. The potential genes for the anti-cancer role of ZNF24, involving transcriptional regulation of several factors, such as dual-specificity phosphatase 1 and squalene epoxidase. E2 conjugating enzyme UBC9 and small ubiquitin-like modifier (SUMO) 1 were found to interact with ZNF24, suggesting that ZNF24 may be SUMOylated. Consistent with the expression, ZNF24 SUMOylation levels were decreased in BC cells and tissues. Pan-SUMOylation inhibition promoted protein degradation of ZNF24. UBC9 SUMOylated ZNF24 at Lys-27 (K27) site with SUMO1 modification and the K27 mutation of ZNF24 greatly damaged the protein stability of ZNF24. Cullin 3 (CUL3), a E3 ubiquitin ligase, was responsible for the degradation of ZNF24. ZNF24 SUMOylation prevented CUL3-mediated protein degradation of ZNF24. Overall, the crosstalk between the SUMOylation and ubiquitination of ZNF24 may be a novel regulatory mechanism to block tumorigenesis and development of BC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Products and Bioprospecting
·
2025
·Vol. 15
·期 1
·1-8
·
IF 4.9
Wang Zi-Jiao,Tan Bang-Yin,Zhao Yun,Wang Chang-Bin,Zhao Yun-Li,Luo Xiao-Dong
Two new together with 32 known compounds were isolated from the leaves of Lycium barbarum . Their structures were elucidated using 1D and 2D NMR, HRESIMS, and ECD spectroscopic techniques. Compounds 1–34 were evaluated for their anti-rheumatoid arthritis activities in a lipopolysaccharide (LPS)-induced MH7A cells inflammatory model. As a result, compounds 1– 3 , 6 , 8 , 10 , 14 , 17–19 , 29 and 31 inhibited the activity of lactate dehydrogenase (LDH) and nitric oxide (NO) at concentrations 20 μM. Among them, compound 1 showed the best effectiveness, with inhibition rates of 46.7% for NO and 32.8% for LDH. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）