LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Jin Wang,Qijun Wang,Liang Xu,Da-Wen Sun
Effects of extremely low frequency pulsed electric field (ELF-PEF) on the total volatile basic nitrogen (TVB-N), thiobarbituric acid reactive substance (TBARS), total microorganism number, protein oxidation and denaturation, enzymatic activity, microstructure, and sensory quality of tilapia fillets during cold storage at 4°C for 6 days are evaluated. The results show that the TVB-N value and total microorganism number in the treated group are within the international standard (< 20 mg/100 g, < 7 log 10 CFU/g) during the storage, and the shelf-life of the treated group is extended by 1 - 2 days as compared with the control. In addition, ELF-PEF treatment inhibits sample quality deterioration during storage, as reflected by a small change in colour, slow softening of texture, less protein oxidation and denaturation, lower endogenous enzyme activity, and tighter microstructure compared to the control, indicating the promising of ELF-PEF treatments for fish preservation during cold storage.
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Materials Today Nano
·
2022
·
IF 13.364
X. Cui,Y. Zhou,Y. Zheng,L. Cao,L. Gao,J. Duan,H. Yin,S. Ai
N 6 -methyladenosine (m 6 A) is the most abundant and dynamic reversible modification of mRNA in eukaryotes discovered in the mid-1970s, which is widely involved in various physiological processes. Fat mass and obesity-associated protein (FTO protein) is not only a demethylase for m 6 A, but also a carcinogenic factor for many human cancers. Therefore, it is very important to build a simple and sensitive platform for simultaneous detection of m 6 A and FTO protein. To achieve this aim, a novel photoelectrochemical biosensor was constructed with the antibody-free detection strategy of DTT mediated –SH functionalization of m 6 A triggered by FTO protein catalyzed m 6 A oxidation . To achieve high detection sensitivity, CdS/Bi 2 MoO 6 /MoSe 2 system with matched energy gap was employed as photoactive material. To achieve high detection selectivity , the –CH 3 of m 6 A in the captured RNA sequence (m 6 A RNA) on the electrode surface was oxidized by FTO protein to form hydroxymethyl, followed with the covalent reaction of DTT with hydroxymethyl, making the labeling of m 6 A with –SH. Finally, a CdS nanoparticle was modified on the electrode through the specific covalent reaction between –SH and Cd, achieving the improvement of the photocurrent with the matched energy band . Under the optimal experimental conditions, the biosensor showed linear range from 0.001 to 50 nM and 0.0005–500 μg/L with the detection limit of 0.37 pM and 0.034 ng/L (S/N = 3) for m 6 A RNA and FTO protein, respectively. The applicability of the developed method was assessed by investigating the effect of entacapone on FTO protein activity.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 18
·10743
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IF 6.208
Teng Wang,Xi Hu,Yujun Yang,Qing Wu,Chengdian He,Xiong He,Zhenyu Wang,Xiang Mao
Metal-based enzyme mimics are considered to be acceptable agents in terms of their biomedical and biological properties; among them, iron oxides (Fe3O4) are treated as basement in fabricating heterogeneous composites through variable valency integrations. In this work, we have established a facile approach for constructing Fe3O4@Ag composite through assembling Fe3O4and Ag together via polyethyleneimine ethylenediamine (PEI) linkages. The obtained Fe3O4@PEI@Ag structure conveys several hundred nanometers (~150 nm). The absorption peak at 652 nm is utilized for confirming the peroxidase-like activity of Fe3O4@PEI@Ag structure by catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The Michaelis–Menten parameters (Km) of 1.192 mM and 0.302 mM show the higher catalytic activity and strong affinity toward H2O2and TMB, respectively. The maximum velocity (Vmax) value of 1.299 × 10−7M∙s−1and 1.163 × 10−7M∙s−1confirm the efficiency of Fe3O4@PEI@Ag structure. The biocompatibility illustrates almost 100% cell viability. Being treated as one simple colorimetric sensor, it shows relative selectivity and sensitivity toward the detection of glucose based on glucose oxidase. By using indocyanine green (ICG) molecule as an additional factor, a remarkable temperature elevation is observed in Fe3O4@PEI@Ag@ICG with increments of 21.6 °C, and the absorption peak is nearby 870 nm. This implies that the multifunctional Fe3O4@PEI@Ag structure could be an alternative substrate for formatting acceptable agents in biomedicine and biotechnology with enzymatic and photothermal properties.Keywords:Fe3O4@PEI@Ag;assembling process;enzymatic;catalytic performance;photothermal property
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ACS Applied Materials & Interfaces
·
2022
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IF 10.383
Ke Yang,Xueyao Zhou,Zhaoli Li,Zefeng Wang,Yuze Luo,Le Deng,Dinggeng He
Preventing bacterial infections and accelerating wound closure are essential in the process of wound healing. Current wound dressings lack enough mechanical properties, self-healing ability, and tissue adhesiveness, and the bacterial killing also relies on the use of antibiotic drugs. Herein, a well-designed hybrid hydrogel dressing is constructed by simple copolymerization of acrylamide (AM), 3-acrylamido phenylboronic acid (AAPBA), chitosan (CS), and the nanoscale tannic acid (TA)/ferric ion (Fe3+) complex (TFe). The resulting hydrogel possesses lots of free catechol, phenylboronic acid, amine, and hydroxyl groups and contains many reversible and dynamic bonds such as multiple hydrogen bonds and boronate ester bonds, thereby showing satisfactory mechanical properties, fast self-healing ability, and desirable tissue-adhesive performance. Benefiting from the high photothermal conversion efficiency of the TFe, the hydrogel exhibits satisfactory antibacterial activity against both Gram-positive and Gram-negative bacteria. Moreover, the embedded TFe also endows the hydrogel with good antioxidant activity, anti-inflammatory property, and cell proliferation to promote tissue regeneration. Remarkably, in vivo animal assays reveal that the hybrid hydrogel effectively eliminates biofilm bacteria in the wound sites and accelerates the healing process of infected wounds. Taken together, the developed versatile hydrogels overcome the shortcomings of traditional wound dressings and are expected to become potential antibacterial dressings for future biomedical applications.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2022
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IF 6.76
Xiaolei Zhang,Ziying Wang,Yangsai Lyu,Jia Li,Kun Song,Ningning Xing,Dickon H.L. Ng
Near-infrared (NIR) light-powered nanomotors with active motion have attracted extensive attention in the chemo-photothermal therapy of cancer. However, there are also some limitations such as complicated synthesis process, low permeability of drugs and viscous physiological environment. Herein, we developed a facile route to synthesize novel NIR light-powered DOX/Au-m-HTAS nanomotor based on natural halloysite (HNTs) for active chemo-photothermal therapy. Such nanomotor exhibited NIR light controlled on/off motion with a maximum speed of 64.5 μm/s. Owing to the synergetic NIR light-powered motion and chemo-photothermal therapy, DOX/Au-m-HTAS nanomotor could effectively adhere tumor cells, leading to deeper cell penetration, higher cellular uptake, and better ablation of tumor cells. Meanwhile, DOX/Au-m-HTAS nanomotors also had enhanced ability for CT imaging of local tumor, demonstrating the enhanced anticancer efficiency. This strategy provided a new insight into the chemo-photothermal synergistic therapy of tumors.
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Frontiers in Environmental Science
·
2022
·
IF 5.411
Han Qi,Dong Wenyi,Wang Hongjie,Yu Boping,Dong Zijun,Li Mu,Xie Linshen,Dai Zhiguang
In this study, the degradation of bisphenol A (BPA) by ozonation was studied systematically by investigating the effects of different factors, including ozone dosages (0.25–1.50 mg/L), temperatures (10–50°C), initial solution pH (3.0–11.0) and interfering ions. The reaction kinetics were analyzed at the same time. In addition, the generation of intermediates was analyzed and the possible mechanism was proposed by combining with the density functional theory (DFT) calculation. At last, the variation and controlling effect of toxicity was also evaluated. The results showed that ozonation had a stronger degrading ability of BPA (1.0 mg/L). A complete removal efficiency was obtained within 10 min when dosing only 1.0 mg/L ozone. The BPA degradation reactions were well fitted with pseudo-second-order kinetics and could well adapt with the wide range of pH (3.0–9.0), during which over 91% removal of BPA was achieved. The indirect pathway by •OH oxidation was proved mainly responsible for BPA degradation by the scavenging and electron paramagnetic resonance experiments. HCO3−, NH4+ and humic acid showed a certain inhibiting effect. Fe3+ and Cu2+ played a catalytic role on BPA degradation. The DFT calculation has identified that the active regions of BPA was focused at C6, C4, C5 and C1 in terms of radical and electrophilic attack. Thus, combining the results of DFT with GC/MS-MS detection, two degradation pathways of BPA were proposed. Toxicity evaluation showed that, due to the generation and accumulation of more toxic intermediates, values of T% had a sharp increase at initial stage. However, with ozone dosage increasing to 1.50 mg/L, the toxicity could be effectively controlled much lower (5%) than BPA itself (49%).
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ANALYTICA CHIMICA ACTA
·
2022
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IF 6.911
Jie Xiang,Junyi Zhang,Shunmei Li,Ruo Yuan,Yun Xiang
The concentration variation of phenylalanine (Phe), an essential amino acid in humans, can cause metabolism disorders and even mental disability. Sensitive and convenient monitoring of Phe is therefore important for disease diagnosis. We describe here the establishment of a new aptamer-based, sensitive and label-free colorimetric Phe detection strategy by integrating catalytic hairpin assembly (CHA) and Mg 2+ -dependent DNAzyme amplification cascades. The target Phe coordinates with pentamethylcyclopentadienyl rhodium(III) chloride dimer [(Cp*RhCl 2 ) 2 ] to form a complex that has a high affinity to the corresponding aptamer sequence. Upon its binding to the aptamers in DNA duplex probes, ssDNA strands are released to trigger subsequent CHA reactions for the formation of many DNAzymes, which cleave the substrate signal probes to liberate lots of CHA initiation strands and free G-quadruplexes to realize the cascaded amplifications. Hemin further associates with the many G-quadruplexes to yield hemin/G-quadruplex mimicking peroxidases , which catalyze solution of substrate to exhibit highly enhanced UV–vis adsorption for detecting Phe at 0.19 μM level. At the meantime, the monitoring of Phe in diluted serums with high selectivity has also been demonstrated by the developed method, indicating its potential for simple diagnosis of Phe-related diseases.
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ENVIRONMENTAL POLLUTION
·
2022
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IF 9.988
Lulu Wang,Laihong Shen,Yuyang Long,Dongsheng Shen,Shouxi Jiang
Uncoupling chemical looping gasification (CLG), the organic sulfur evolution was simulated and explored qualitatively and quantitatively using typical sulfur compounds on TG-MS and temperature-programmed fixed bed. The HS radical in the reductive atmosphere easier converted to H 2 S and COS. H 2 O activated the evolution of S which was stably bonded to carbon, and H 2 generated from gasification and oxidation of reductive Fe by H 2 O contributed to the release of sulfur. The proportion of H 2 S released from sulfur compounds was greater than 87% in steam gasification, and more than 60% during CLG. Oxygen carriers promoted the conversion of sulfur to SO 2 in the mid-temperature region (500 °C–700 °C), and H 2 S in the high temperature region (700 °C–900 °C). Sulfur species played a pivotal role in sulfur evolution at low temperature of CLG. The organic sulfur in mercaptan and benzyl were more easily converted and escaped than in thiophene and phenyl . The thermal stability of sulfur species, the presence of steam and OC affected the initial temperature and peak concentration of gas sulfur release as well as sulfur distribution. Consequently, CLG strengthened the sulfur evolution, and made it possible to targeted restructure the distribution of sulfur by regulating process parameters, or blending fuel with different sulfur species for emission reduction, and selective conversion of sulfur.
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JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·Vol. 77
·期 9
·1141-1146
·
IF 1.237
Peng Fangfang,Zhou Xin,Cheng Wanmin,Ma Jimei,Jiang Hong
Abstract Carbon dots ( CDs ) with yellow-green fluorescence were synthesized by using m -aminophenol and ethylenediamine under mild conditions and applied as a sensor for detecting cysteine ( Cys ). Cys reacted with p -nitrophenyl acrylate via Michael addition and cyclization to form p -nitrophenol ( PNP ) which had the UV absorption maximum at 405 nm. PNP quenched the fluorescence of CDs at the excitation wavelength of 420 nm based on inner filter effect. The fluorescence intensity change of CDs was used as an output signal for Cys detection. The probe detected Cys over homocysteine and glutathione in a solution with high selectivity. The system was highly sensitive to Cys with low limit of detection of 0.8 μM and showed a good linear relationship in the range of 1–50 μM. This carbon dots probe has the potential to be applied in biological analysis.
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EXPERIMENTAL AND MOLECULAR MEDICINE
·
2023
·Vol. 55
·期 1
·183-195
·
IF 12.8
Wu Zhuhua,Zhu Li,Nie Xinran,Wei Li,Qi Yong
Pulmonary hypertension (PH) is a life-threatening cardiopulmonary disease characterized by pulmonary vascular remodeling. Excessive growth and migration of pulmonary artery smooth muscle cells (PASMCs) are believed to be major contributors to pulmonary vascular remodeling. Ubiquitin-specific protease 15 (USP15) is a vital deubiquitinase that has been shown to be critically involved in many pathologies. However, the effect of USP15 on PH has not yet been explored. In this study, the upregulation of USP15 was identified in the lungs of PH patients, mice with SU5416/hypoxia (SuHx)-induced PH and rats with monocrotaline (MCT)-induced PH. Moreover, adeno-associated virus-mediated functional loss of USP15 markedly alleviated PH exacerbation in SuHx-induced mice and MCT-induced rats. In addition, the abnormal upregulation and nuclear translocation of YAP1/TAZ was validated after PH modeling. Human pulmonary artery smooth muscle cells (hPASMCs) were exposed to hypoxia to mimic PH in vitro, and USP15 knockdown significantly inhibited cell proliferation, migration, and YAP1/TAZ signaling in hypoxic hPASMCs. Rescue assays further suggested that USP15 promoted hPASMC proliferation and migration in a YAP1/TAZ-dependent manner. Coimmunoprecipitation assays indicated that USP15 could interact with YAP1, while TAZ bound to USP15 after hypoxia treatment. We further determined that USP15 stabilized YAP1 by inhibiting the K48-linked ubiquitination of YAP1. In summary, our findings reveal the regulatory role of USP15 in PH progression and provide novel insights into the pathogenesis of PH.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Yongshuai Jing,Shilin Zhang,Feifan Wang,Beibei Hu,Shiguo Sun,Danshen Zhang,Yuguang Zheng,Lanfang Wu
The polysaccharides of Salvia miltiorrhiza (SMP) from 10 different sources were obtained by water extraction and alcohol precipitation, and their physicochemical properties and structures were analyzed. Multidimensional fingerprints based on high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC) and proton nuclear magnetic resonance ( 1 H-NMR) were established, and monosaccharide composition (galactose, glucose), molecular weight (SP1 2.07 × 10 6 Da-5.57 × 10 6 Da, SP3 692.20 Da–707.22 Da) and chemical shift (δ2 4.982 ppm, δ4 5.317 ppm) could be used for the quality control of SMP. The antioxidant activity was evaluated, and the spectrum effect relationship was studied. Galactose in the monosaccharide composition and the 2.07 × 10 6 Da-5.57 × 10 6 Da component in molecular weight were found to have a positive correlation effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, which can provide a preliminary basis and data reference for the quality control and structure-activity relationship study of SMP.
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ANALYST
·
2023
·Vol. 148
·期 4
·912-918
·
IF 4.2
Xinai Zhang,Zhenzhong Wang,Xu Li,Wei Xiao,Xiaobo Zou,Qilin Huang,Lili Zhou
The development of effective methods for tracking cancer cells is of significant importance in the early diagnosis and treatment of tumor diseases. Compared with the developed techniques, the electrochemical assay has shown considerable potential for monitoring glycan expression on the cell surface using nondestructive means. However, the application expansion of the electrochemical strategy is strongly impeded owing to its dependence on electroactive species. In this study, a competitive electrochemical strategy was reported for monitoring cancer cells based on mannose (a typical glycan) as a clinical biomarker. Herein, functionalized carbon nanotubes were used to load the thiomannosyl dimer, and thionine-interlinking signal probes were designed for competitive recognition. After effective competition between cancer cells and the anchored mannose, a decreased current was obtained as the cell concentration increased. Under optimal conditions, the proposed biosensor exhibited attractive performance for cancer cell analysis with a detection limit as low as 20 cells per mL for QGY-7701 and 35 cells per mL for QGY-7703, facilitating great promise for the sensitive detection of cancer cells and thus showing potential applications in cancer diagnosis.
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