TALANTA
·
2020
·
IF 5.339
Huilan Piao,Yanxiao Jiang,Zucheng Qin,Pinyi Ma,Ying Sun,Xinghua Wang,Daqian Song,Qiang Fei
In this work, a rapid, convenient, sensitive, and cost-effective in-situ formed magnetic deep eutectic solvent based on a dispersive liquid-liquid extraction method was developed for the determination of triazine herbicides in rice. A novel tetrabutylammonium chloride (TBAC) based magnetic deep eutectic solvent (MDES), [TBAC/ethylene glycol][FeCl 4 ] was generated by means of an simple in-situ reaction of the deep eutectic solvent (DES) with iron chloride in the sample solution. The solvent which has high affinity for target analytes and magnetic ability was successfully employed as an extraction solvent and simplified both the extraction and separation procedure. A series of parameters influencing the extraction efficiency were investigated. Under optimal conditions, calibration curves for the target triazine herbicides were obtained in the ranges of 5–1000 ng g −1 , with correlation coefficients greater than 0.9910. The limits of detection (LOD) and quantification (LOQ) were in the range of 1.49–3.10 ng g −1 and 4.96–10.34 ng g −1 , respectively. The precision of intra- and inter-day were under 6.2% and 9.6%, respectively, and the precision between laboratories were lower than 7.5%. The accuracy of the method varied from 84.9 to 117.5%. Furthermore, the method showed satisfactory matrix effect and robustness. These results indicate that the technique is suitable for rice sample analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2020
·Vol. 66
·期 11
·e17015
·
IF 3.519
Caijin Zhou,Bingqi Xie,Shaowei Li,Jisong Zhang
In this work, an unsteady-state strategy for rapid measurement of gas diffusivity in liquid is proposed, which has a quick perturbation of the liquid flow rate in inner tube for obtaining the change of gas flow rate across the membrane with time. The strategy has taken full advantages of the tube-in-tube reactor that possesses a high permeability Teflon AF-2400 membrane to accelerate the diffusion rate of gas to liquid without direct contact between the two phases. With a developed mathematical model fitting the recorded variation of gas flow rate with time, the gas diffusivity in liquid can be determined within 0.5–3 min compared with the conventional methods of 4–14 hr. In addition, the strategy is demonstrated with several gas–liquid systems (O 2 -DMSO, CO 2 -[Emim] [NTf 2 ] and CO 2 -[Bmim] [BF 4 ]) with varied viscosities and temperatures, showing a good agreement with literature values with less than 10% deviation.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF EXPERIMENTAL BOTANY
·
2020
·Vol. 71
·期 19
·5948-5962
·
IF 5.908
Deng Changping,Shi Min,Fu Rong,Zhang Yi,Wang Qiang,Zhou Yang,Wang Yao,Ma Xingyuan,Kai Guoyin
Phenolic acids and tanshinones are major bioactive ingredients in Salvia miltiorrhiza, which possess pharmacological activities with great market demand. However, transcriptional regulation of phenolic acid and tanshinone biosynthesis remains poorly understood. Here, a basic leucine zipper transcription factor (TF) named SmbZIP1 was screened from the abscisic acid (ABA)-induced transcriptome library. Overexpression of SmbZIP1 positively promoted phenolic acid biosynthesis by enhancing expression of biosynthetic genes such as cinnamate-4-hydroxylase (C4H1). Furthermore, biochemical experiments revealed that SmbZIP1 bound the G-Box-like1 element in the promoter of the C4H1 gene. Meanwhile, SmbZIP1 inhibited accumulation of tanshinones mainly by suppressing the expression of biosynthetic genes including geranylgeranyl diphosphate synthase (GGPPS) which was confirmed as a target gene by in vitro and in vivo experiments. In contrast, the phenolic acid content was reduced and tanshinone was enhanced in CRISPR/Cas9 [clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9]-mediated knockout lines. In addition, the previously reported positive regulator of tanshinone biosynthesis, SmERF1L1, was found to be inhibited in SmbZIP1 overexpression lines indicated by RNA sequencing, and was proven to be the target of SmbZIP1. In summary, this work uncovers a novel regulator and deepens our understanding of the transcriptional and regulatory mechanisms of phenolic acid and tanshinone biosynthesis, and also sheds new light on metabolic engineering in S. miltiorrhiza.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Tian Yao,Kaifeng Du
Four novel organic magnetic ionic liquids were synthesized and characterized. A new method of in-situ magnetic ionic liquid dispersive liquid-liquid microextraction coupled with HPLC was established to simultaneously separate, preconcentrate and determine trace amount of sulfonamides in milk samples for the first time. In this work, extraction procedure was free of volatile organic solvent. Extraction equilibrium was immediately achieved without further operation such as vortex shaking. Magnetic separation of two phases was effectively achieved by applying external magnetic field without need of centrifugation. Furthermore, only a very small amount of inorganic salts were generated. This is the first time to realize all these advantages in an analytical method. Under the optimal conditions, satisfactory enrichment factors, precisions, linear range and low limit of detection and quantitation were acquired. The validated method was successfully applied in the simultaneous analysis of 5 sulfonamides in real milk samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Yiye Wang,Xueyan Luo,Ye Han,Baijun Liu,Yiwei Hu,Xiangchi Liu
Styrene emulsion polymerizations using sodium dodecyl sulfate as an anionic surfactant and in the presence of cationic initiators 2, 2′- Azobis (2-methylpropionamidine) dihydrochloride (AIBA) and 2,2′-azobis(N,N′-dimethyleneisobutyramidine) dihydrochloride (ADIBA) were studied. In this work, an explanation for in situ charge neutralization is proposed, and the effect of in situ charge neutralization on the particle size distribution of the ultimate polymer particles is discussed. Charges of cationic radicals from initiator decomposition shield the anionic charges of surfactant molecules adsorbed on the particle surface and induce particle coagulation during the early particle formation period. As the occurrence of in situ charge neutralization, the particle number decreases and the rate of micelle capture for the initiator radical is enhanced. Thus, the particle nucleation period is shortened, and monodisperse polymer particles with a diameter of ～200−300 nm are prepared using this simple emulsion polymerization process. In addition, hydrolysis of the initiator AIBA at different pH conditions is used to control particle size and stability. Compared to conventional emulsion polymerization theories, the emulsion polymerization in the presence of in situ charge neutralization presents unique kinetics.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Orthopaedic Translation
·
2019
·
IF 3.063
Yong Dai,Han Guo,Linyang Chu,Zihao He,Minqi Wang,Shuhong Zhang,Xifu Shang
Objective To enhance the bioactivity of polyetheretherketone (PEEK) while maintain its mechanical strengths. Methods Suspension coating and melt bonding. Results Silicon nitride (Si 3 N 4 , SN) coating lead to higher surface roughness, hydrophilicity and protein absorption; SN coating could slowly release Si ion into simulated body fluid (SBF), which caused weak alkaline of micro-environment owing to the slight dissolution of SN; SN coating resulted in the improvements of adhesion, proliferation, differentiation and gene expressions of MC3T3-E1 cells in vitro ; SN coating of PEEK with bioactive SN coating (CSNPK) obviously promoted bone regeneration and osseointegration in vivo . Conclusions CSNPK with SN coating as bone implant might be a promising candidate for orthopedic implants. The Translational Potential of this Article The silicon nitride-coated polyetheretherketone (CSNPK) prepared in this article could induce MC3T3-E1 cells adhesion, proliferation and differentiation in vitro ; it could also induce bone regeneration in bone defect in vivo , which indicate its good cytocompatibility and biocompatibility. If the raw materials are medical grade, and preparation process as well as production process of this article are further improved, it will have great translational potential.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POWDER TECHNOLOGY
·
2019
·
IF 3.413
Xuechen Tang,Wanli Kang,Bobo Zhou,Yongbo Gao,Changxiao Cao,Shujun Guo,Muhammad Wassem Iqbal,Hongbin Yang
Polymer/nano-SiO 2 composite microspheres (PNSCMs) were synthesized via inverse suspension polymerization with 3-methacryloxypropyltrimethoxysilane (MPS) modified SiO 2 as the reinforcing filler. The influences of these polymerizable nanoparticles on the swelling behavior, mechanical property, thermal stability, viscoelasticity and dispersion stability were examined in detail. Moreover, the plugging behaviors were investigated by sand pack tests. The results indicate that the introduction of these modified nanoparticles revealed a significant enhancement on the comprehensive properties of PNSCMs. By varying the content of MPS-modified SiO 2 , the environmental sensitivity of the microspheres’ swelling behavior was reduced. Besides, the mechanical property, thermal stability and viscoelasticity were greatly improved by increasing the SiO 2 loading. Compared with SiO 2 -free ones, PNSCMs exhibited better dispersion stability and plugging performance, and the maximum plugging rate reached 93.64%, which was increased by 14.69%. These PNSCMs studied can realize in-depth profile control in reservoirs and have great application prospects in the further enhanced oil recovery research.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2022
·
IF 3.656
Meng Chen,Chao Wang,Lei Wang,Jixiang Xu
In this work, carbon quantum dots@In 2 S 3 hollow tube (CQDs@In 2 S 3 ) with sheet-like hierarchical structure was synthesized by sulfidation CQDs@In-MIL-68. The CQDs@In 2 S 3 -2 exhibited the optimal Cr(VI) reductive ratio of nearly 100% after 45 min light irradiation in solution with pH of 4. The enhanced Cr(VI) reduction activity was ascribed to the efficient separation of electrons and holes after coupling of CQDs. The ·O 2 − was the dominant active specie to reduce Cr(VI), and possible mechanism was proposed. This work provides thought for design of high-performance photocatalysts by loading electron-collecting species to remove environmental pollutants .
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 148
·期 4
·752-761
·
IF 5.227
Xintong Lu,Luoyu Ding,Haohan Song,Wenxin Yu,Chaoqing Dong,Jicun Ren
Matrix metalloproteinase-9 (MMP-9) plays an important role in tumor progression. It is of great significance to establish a sensitive in situ assay strategy for MMP-9 activity in single living cells. Here a novel in situ single molecule spectroscopy method based on the fluorescence correlation spectroscopy (FCS) technique was proposed for measuring the MMP-9 activity at different locations within single living cells, using a fluorescent specific peptide and a reference dye as dual probes. The measurement principle is based on the decrease of the ratiometric translational diffusion time of dual probes in the detection volume due to the peptide cleavage caused by MMP-9. The peptide probe was designed to be composed of an MMP-9 cleavage and cell-penetrating peptide sequence that was labeled with a fluorophore and conjugated with a streptavidin (SAV) molecule. The ratiometric translational diffusion time was used as the measurement parameter to eliminate the effect of intracellular uncertain viscosity. The linear relationship between the ratiometric diffusion time and MMP-9 activity was established, and applied to the determination of enzymatic activity in cell lysates as well as the evaluation of the inhibitory effects of different inhibitors on MMP-9. More importantly, the method was successfully used to dynamically determine MMP-9 activity in single living cells or under the stimulation with phorbol 12-myristate 13-acetate (PMA) and inhibitors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jinjie Xu,Yuehong Pang,Zhuying Yan,Xiaofang Shen
Glyphosate, a widely used herbicide, is prone to residue in the environment that may cause harm to humans. In this work, titanium ion-modified melamine foams (MF/PDA/Ti 4+ ) were prepared as sorbents to achieve rapid extraction of glyphosate by pipette-tip solid-phase extraction (PTSPE). The MF/PDA/Ti 4+ was characterized by SEM, EDS, XPS, and TGA, and its adsorption properties were evaluated by adsorption kinetics and isotherm experiments. The porous structure and good mechanical properties of MF made the MF/PDA/Ti 4+ fill into the pipette-tip easily and could achieve low back pressure. The developed PTSPE method showcased short extraction time (<2 min) for glyphosate and the limits of detection (LOD, 3σ/k) was 4.9 μg/L. It was applied in aqueous samples (tap water, lake water, tea drink, and orange juice) with recoveries ranging from 82.8 % to 108.2 % and the relative standard deviation (RSD, n = 3) was less than 7.7 %, demonstrating broad perspectives on the extraction of glyphosate from the aquatic and terrestrial environment.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Oncology
·
2020
·
IF 4.848
Zhan Wenhua,Li Hanrui,Guo Yingying,Du Getao,Wu Yayan,Zhang Dexin
Combination chemotherapy is a routine treatment for esophageal cancer, but some shortcomings, such as drug toxicity and side effects, greatly limit the clinical application of combination therapy. To overcome these shortcomings, we have developed a mesoporous silica nanoparticle system that was used to load doxorubicin and β-elemene. β-elemene was encapsulated in the pore of mesoporous silica nanoparticle and doxorubicin was electrostatically adsorbed on the surface of mesoporous silica nanoparticle by hyaluronic acid to construct dual drugs synergistic nanoparticles (bMED NPs, ~77.15 nm). In vitro studies demonstrated that bMED NPs had a good treatment effect in esophageal cancer cell lines. In vivo fluorescence imaging results demonstrated that bMED NPs could accumulate in tumor sites and achieve in vivo long-term circulation and continuous drug release. In addition, bMED NPs exhibited significant antitumor effects in the esophageal cancer mouse model, which may provide a great platform for esophageal cancer chemotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 43
·8980-8993
·
IF 6.331
Shuying Hu,Hanbang Chen,Fang Zhou,Jun Liu,Yunzhu Qian,Ke Hu,Jia Yan,Zhuxiao Gu,Zhaobin Guo,Feimin Zhang,Ning Gu
Bone tissue engineering (BTE) is a promising approach to recover insufficient bone in dental implantations. However, the clinical application of BTE scaffolds is limited by their low mechanical strength and lack of osteoinduction. In an attempt to circumvent these limitations and improve osteogenesis, we introduced magnetic iron oxide nanoparticles (IONPs) into a core–shell porous electrospun scaffold and evaluated their impact on the physical, mechanical, and biological properties of the scaffold. We used poly(lactic-co-glycolic acid)/polycaprolactone/beta-tricalcium phosphate (PPT) scaffolds with and without γ-Fe2O3 encapsulation, namely PPT–Fe scaffolds and PPT scaffolds, respectively. The γ-Fe2O3 used in the PPT–Fe scaffolds was coated with polyglucose sorbitol carboxymethylether and was biocompatible. Structurally, PPT–Fe scaffolds showed uniform iron distribution encapsulated within the resorbable PPT scaffolds, and these scaffolds supported sustainable iron release. Furthermore, compared with PPT scaffolds, PPT–Fe scaffolds showed significantly better physical and mechanical properties, including wettability, superparamagnetism, hardness, tensile strength, and elasticity modulus. In vitro tests of rat adipose-derived mesenchymal stem cells (rADSCs) seeded onto the scaffolds showed increased expression of integrin β1, alkaline phosphatase, and osteogenesis-related genes. In addition, enhanced in vivo bone regeneration was observed after implanting PPT–Fe scaffolds in rat calvarial bone defects. Thus, we can conclude that the incorporation of IONPs into porous scaffolds for long-term release can provide a new strategy for BTE scaffold optimization and is a promising approach that can offer enhanced osteogenic capacity in clinical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）