FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Maoyun Li,Xiaoqin Luo,Ruixue Zhu,Kai Zhong,Wenyi Ran,Yanping Wu,Hong Gao
The development of bio-based and active food packaging materials is an effective way to alleviate the growing environmental concern and extend the shelf life of food . This study aimed to prepare a novel bilayer film composed of chitosan (CS) and sodium alginate (SA) incorporated with inclusion complex of dihydromyricetin with hydroxypropyl-β-cyclodextrin (ICDH) by layer-by-layer casting. The results of structural and physical characterization revealed that CS/SA/ICDH films were hydrophilic and had good compatibility, thermal stability, and UV blocking capability, with a smooth and uniform appearance. Compared with CS/SA film, CS/SA/ICDH-10% film exhibited improved tensile strength (45.57 MPa) and elongation at break (22.46%). More importantly, incorporation of ICDH was shown to enhance the antioxidant activity of the bilayer film, as well as the antibacterial activity against Staphylococcus aureus . CS/SA/ICDH-10% film had a superior performance as a food packaging material on preservation of fried meatballs by effectively reducing lipid oxidation and microbial growth. After storage for 7 days, the thiobarbituric acid reactive substance (TBARS) and total plate count (TPC) of fried meatballs packaged with CS/SA/ICDH-10% film were 31.72 μg/g and 3.29 log 10 CFU/g, respectively, which were significantly lower than those of the control group (37.00 μg/g, 5.34 log 10 CFU/g) and plastic film group (41.07 μg/g, 5.97 log 10 CFU/g). Therefore, this study suggested that CS/SA/ICDH film could be used as a promising active packaging material for application in food industry.
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ADVANCED SYNTHESIS & CATALYSIS
·
2018
·Vol. 361
·期 3
·556-561
·
IF 5.123
Peiyuan Yao,Zefei Xu,Shanshan Yu,Qiaqing Wu,Dunming Zhu
The morphinan skeleton is an important sub-structure in many medicines such as dextromethorphan, and can be constructed from 1-benzyl-1,2,3,4,5,6,7,8-octahydroisoquinoline (1-benzyl-OHIQ) derivatives. 1-Benzyl-3,4,5,6,7,8-hexahydroisoquinolines (1-benzyl-HHIQs), the precursors of 1-benzyl-OHIQs, constitute a type of bulky α, β-unsaturated imines. Until now, the application of imine reductases (IREDs) to α, β-unsaturated imines has only rarely been reported. In this study, through evaluation of 48 IREDs, both enantiomers of 1-(4-methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline (1-(4-methoxybenzyl)-OHIQ) were obtained in high yield and excellent optical purity. Among the enzymes, the most steric hindrance-tolerant IRED from Sandarearacinus amylolyticus (IR40) was able to convert various phenyl substituted 1-benzyl-HHIQ to the corresponding 1-benzyl-OHIQ derivatives with excellent enantiometric excess. These results provide an effective route to synthesize these important compounds via enantioselective reduction of bulky α, β-unsaturated imine precursors, which can be readily prepared from 2-(1-cyclohexenyl)ethylamine and corresponding aryl acetic acids.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 4
·1-8
·
IF 5.479
Yang Xuan,Sun Xiaodong,Feng Zijie,Du Yingxiang,Chen Jiaquan,Ma Xiaofei,Li Xiaoqi
Magnetic nanoparticles (MNPs) modified with β-cyclodextrin and mono-6-deoxy-6-(1-methylimidazolium)-β-cyclodextrin tosylate (an ionic liquid), which called MNP-β-CD and MNP-β-CD-IL, were coated into the capillary inner wall. Compared to an uncoated capillary, the new systems show good reproducibility and durability. The systems based on the use of MNP-β-CD or MNP-β-CD-IL as stationary phases were established for enantioseparation of Dns-modified amino acids. Improved resolutions were obtained for both CEC systems. Primary parameters such as running buffer pH value and applied voltage were systematically optimized in order to obtain optimal enantioseparations. Under the optimized conditions, the capillaries exhibited excellent chiral recognition ability for six Dns-amino acids (the DL-forms of alanine, leucine, lsoleucine, valine, methionine, glutamic acid) and provided a promising way for the preparation of chiral column.
Schematic presentation of the open-tubular capillary electrochromatography systems with MNP-β-CD and MNP-β-CD-IL as stationary phases for enantioseparation of dansylated amino acids.
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PubMed
高影响因子
DALTON TRANSACTIONS
·
2016
·Vol. 45
·期 9
·3853-3862
·
IF 4.177
Ao Wang,Weiwei Jin,Enyi Chen,Jiahong Zhou,Lin Zhou,Shaohua Wei
Near-infrared (NIR) light triggered photodynamic therapy (PDT), based on upconversion nanoparticles (UCNPs), has attracted great attention because of its high tissue penetration and low photodamage to living organisms. However, most UCNPs cannot be stably dispersed in aqueous solution and cannot carry photosensitive drugs directly. Besides, UCNP mediated PDT is a fluorescence resonance energy transfer (FRET) process from the UCNPs to the attached photosensitive drugs. So the drug and UCNPs must be stably connected and close enough. In this manuscript, carboxymethyl-β-cyclodextrin (COOH-β-CD) was used to connect UCNPs and adamantine modified phthalocyanine (Ad-ZnPc) through a self-assembly process followed by a host–guest interaction. COOH-β-CD can provide good water solubility of the system and short-distance linking between the UCNPs and Ad-ZnPc. Most importantly, the system has a strong NIR light triggered PDT activity toward cancer cells.
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Zhipeng Xie,Jinlong Lei,Mingfeng Yang,Youji Li,Xinhua Geng,Simin Liu,Jiahai Wang
Amine group-bearing small molecules tend to adsorb onto the nanochannel surfaces, which degrades the efficiency of nanochannel sensors. In this study, we utilized host-guest knowledge to eliminate the influence of excessive small molecules. In combination with single-walled carbon nanotubes (SWNTs), β-cyclodextrin (β-CD) provided an excellent sensing performance for the conical nanochannel coated with polyethyleneimine (PEI) and zirconium ion (Zr 4+ ). By taking detection of melamine as a prototype, the as-prepared nanochannel could selectively detect melamine-elicited double-stranded DNA (dsDNA). Both excessive melamine and single-stranded DNA were removed via the addition of β-CD and SWNTs. The nanochannel sensing platform for the detection of melamine has a detection limit of as low as 4.3 nM with a suitable sensitivity, an excellent reproducibility and stability. Therefore, it is highly promising that a nanochannel sensor based on ion current rectification (ICR) can be used to detect other possible targets.
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2021
·Vol. 32
·期 12
·1-13
·
IF 3.896
Xu Wanghan,Tan Wei,Li Chan,Wu Keke,Zeng Xinyi,Xiao Liwei
Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment. It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis. In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15). The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET. The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro. This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis. After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization. Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis. To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis. Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.
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高影响因子
Biomaterials Advances
·
2022
·
IF 0
Dahu Qi,Jin Su,Song Li,Hao Zhu,Lijin Cheng,Shuaibin Hua,Xi Yuan,Jiawei Jiang,Zixing Shu,Yusheng Shi,Jun Xiao
Bioceramics have been used in orthopedic surgery for several years. Magnesium (Mg) is an essential element in bone tissue and plays an important role in bone metabolism. Mg-doped bioceramics has attracted the attention of researchers recently. However, the optimal doping amount of Mg in β-TCP and the immunomodulatory property of Mg-doped β-TCP (Mg-TCP) have not been determined yet. In this study, β-TCP scaffolds doped with different contents of magnesium oxide (0 wt%, 1 wt%, 3 wt%, and 5 wt%) with gyroid structure were printed by digital light processing (DLP) method, and the physicochemical and biological functions were then investigated. Mg-doping improved the physicochemical properties of the β-TCP scaffolds. In vitro experiments confirmed that the doping of Mg in β-TCP scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and angiogenic differentiation of endothelial progenitor cells (EPCs), where the 5Mg-TCP has the optimal properties when using the “one cell type” method. It was also found that all Mg-TCP facilitated the polarization of RAW264.7 cells to the M2 phenotype, especially the 3Mg-TCP. However, 3Mg-TCP displayed the optimal osteogenic and angiogenic potential when using a “multiple cell type” method, which referred to culturing the BMSCs or EPCs in the macrophage-conditioned medium. Finally, the in vivo experiments were conducted and the results confirmed that the 3Mg-TCP scaffolds possessed the satisfying bone defect repair capability both after 6 and 12 weeks of implantation. This study suggests that 3Mg-TCP scaffolds provide the optimal biological performance and thus have the potential for clinical translation.
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DOI
PubMed
Redox Biology
·
2019
·
IF 7.793
Shen-Fei Wang,Xin Liu,Mo-Yu Ding,Shuangcheng Ma,Jing Zhao,Ying Wang,Shaoping Li
Reducing agents are crucial for the management of maladaptive inflammation-induced macrophage death and hematopoietic toxicity of chemotherapy. 2- O - β - d -glucopyranosyl- l -ascorbic acid (AA-2 β G), a unique AA (or vitamin C) derivative identified in Lycium barbarum , exhibited enhanced free radical scavenging activity compared with AA and its synthetic derivative AA-2 α G. AA-2 β G protected hydrogen peroxide-induced cell death in murine macrophage RAW264.7 cells. Treatment with AA-2 β G eliminated oxidative stress and the ratio of cellular glutathione to glutathione disulfide more effectively than AA and AA-2 α G. AA-2 β G also significantly reduced the fluorescent intensity of DCFH-DA triggered by chemotherapeutic agent camptotehcin-11 but not fluorouracil. AA, AA-2 α G, and AA-2 β G significantly decreased Keap-1expression, and increased the expression levels of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1. All compounds triggered the nuclear translocation of Nrf2, while the ability of AA-2 β G to enhance the Nrf2-DNA binding affinity was approximately two fold as those of AA and AA-2 α G. Sodium ascorbate cotransporters (SVCT) inhibitors, sulfinpyrazone, phloretin, and 3- O -methyglucose, potently abrogated the free radical scavenging activities of AA, AA-2 α G, and AA-2 β G. The cellular uptake efficacy of AA-2 α G and AA-2 β G was less than 10% of AA, while the inhibition of SVCT with sulfinpyrazone considerably diminished the uptake efficacy of these compounds. AA-2 α G and AA-2 β G are more stable in the Fenton reagents than AA. In summary, AA-2 β G from L. barbarum with excellent free radical scavenging activity is a promising natural AA derivative for further pharmacological evaluation.
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PubMed
高影响因子
Desalination and Water Treatment
·
2016
·
IF 1.272
Xudong Zheng,Jiangdong Dai,Jianming Pan
In this work, mesoporous attapulgite (mATP) was firstly prepared in the presence of the cetyltrimethylammonium bromide using attapulgite (ATP) as the matrix materials. Then, it was modified by β-cyclodextrin (β-CD) to obtain the β-cyclodextrin/mesoporous attapulgite composites (β-CD/mATP). The characterization of the composites showed that β-CD/mATP composites remained excellent channel structure and the value of the specific surface area was 300.30 m2/g. As-prepared β-CD/mATP was evaluated as sorbents to adsorption of 2,4,6-trichlorophenol (2,4,6-TCP) and 2,4,5-trichlorophenol (2,4,5-TCP). The results of the batch mode experiment revealed that the Langmuir isotherm model fitted the equilibrium data better, while the kinetic data could be described by pseudo-second-order model perfectly. Furthermore, 2,4,5-TCP adsorption onto β-CD/mATP was more affected than that of 2,4,6-TCP in the presence of competitive phenolic compound. In addition, β-CD/mATP possessed excellent regeneration property.
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高影响因子
RSC Advances
·
2017
·Vol. 7
·期 60
·37636-37642
·
IF 3.108
Qiao-Feng Zheng,Mo Yu,Shu-Shen Liu,Fu Chen
Hormesis is a biphasic concentration–response relationship. During the luminescence inhibition test of Vibrio qinghaiensis sp.-Q67 (Q67), some organic solvents display the hormesis phenomenon. However, the mechanism of hormesis with respect to organic solvents remains unclear. This study focuses on luciferase, which is the key factor in luminescent reactions, and explores its role in the mechanism of hormesis. Q67 luciferase has two subunits, α and β. Molecular docking and molecular dynamics simulations were carried out by taking organic solvents as ligand and the two subunits as receptor. In addition, the binding free energies of the complexes formed by the ligand and Q67 luciferase were calculated. The results showed that the organic solvent ligands exhibiting hormesis bind to the β subunit first, while those that do not exhibit hormesis bind more easily to the α subunit. The hormetic organic solvents bind to β subunit first at low concentration, and change the flexibility of residues Ser145–Arg165 located on the α subunit; this enables flavin mononucleotide (FMN) to bind to the α subunit, exhibiting the hormesis phenomenon. With the increasing concentration, redundant molecules start to bind to the α subunit and compete with/block FMN binding to the α subunit, resulting in inhibition.
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高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Zhixiang Cai,Hongbin Zhang
The chain conformational change in curdlan during carboxymethylation was investigated using nuclear magnetic resonance (NMR), circular dichroism (CD) spectroscopy, and atomic force microscopy (AFM). The distributions of carboxymethyl substituents within anhydroglucose unit (AGU) of CMCD were found to follow the order of OH (6) > OH (4) > OH (2) for CMCD with a low DS and OH (6) > OH (2) > OH (4) for CMCD with relatively high DS. The increased carboxymethylation level induced the chain conformation transition of curdlan from triple helix to random coil in water. The DS of 0.25 was the critical value of chain conformation transition, below which CMCD chains were triple helices. For DS larger than 0.25, CMCD existed in the state of random coils. The intermolecular hydrogen bonding between C2 hydroxyls in AGU sustained the triple helical conformation and stiffness of the polymer chain, which weakened with the increase in DS.
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高影响因子
MOLECULES
·
2018
·Vol. 23
·期 12
·3369
·
IF 3.098
Li-Min Zhao,Zhen Guo,Yi-Jie Xue,Jun Zhe Min,Wen-Jing Zhu,Xiang-Yu Li,Hu-Ri Piao,Cheng Hua Jin
The transforming growth factor-β (TGF-β), in which overexpression has been associated with various diseases, has become an attractive molecular target for the treatment of cancers. Thirty-two quinoxaline-derivatives of 3-substituted-4-(quinoxalin-6-yl) pyrazoles14a–d,15a–d,16a–d,17a–d,18a–d,19a–d,25a,25b,25d,26a,26b,26d,27b, and27dwere synthesized and evaluated for their activin TGF-β type I receptor kinase and p38α mitogen activated protein (MAP) kinase inhibitory activity in enzymatic assays. Among these compounds, the most active compound19binhibited TGF-β type I receptor kinase phosphorylation with an IC50value of 0.28 µM, with 98% inhibition at 10 µM. Compound19balso had good selectivity index of >35 against p38α MAP kinase, with 9.0-fold more selective than clinical candidate, compound3(LY-2157299). A molecular docking study was performed to identify the mechanism of action of the synthesized compounds and their good binding interactions were observed. ADMET prediction of good active compounds showed that these ones possess good pharmacokinetics and drug-likeness behavior.Keywords:TGF-β;ALK5 inhibitor;kinase assay;selectivity;docking
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