BMC Chemistry
·
2026
·
IF 5.1
Shi Xingyi,Cai Huahuan,Tang Wei,Wei Erjun,Zhao MingXiao,Zhao Yufen
The evolutionary origin of copper-binding proteins remains a central question in prebiotic chemistry. Modern copper proteins predominantly coordinate copper through histidine and cysteine residues, suggesting that these amino acids were selectively favored during early protein evolution. Here, we propose that ultraviolet (UV) radiation acted as a key selective factor in shaping primordial copper-peptides. Spectroscopic analyses suggest that His 2 interacts with Cu(II) through contributions from both its N- and C-terminal donor groups, generating a comparatively stronger binding environment and supporting its plausibility as a primitive Cu-binding motif. Yet His 2 is intrinsically vulnerable to UV-induced degradation, highlighting the need for stabilizing partners. In contrast, Tyr 2 exhibits remarkable photostability and preferentially binds Cu(II) through its N-terminal, consistent with a second-shell protective role analogous to those observed in modern Cu-binding proteins. Together, these findings suggest that Earth’s UV-rich environment favored cooperative copper-binding dipeptides, where His₂ provided catalytic functionality while Tyr 2 contributed photostability, establishing a plausible pathway from simple Cu-peptide complexes to functional metalloenzymes.
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高影响因子
Science China-Materials
·
2026
·
IF 7.7
Zeng Jie,Xiong Feilong,Zan Jun,Wang Dongan,Peng Shuping,Yang Hejin,Shuai Cijun
Bacterial infection following endometrial injury would delay tissue regeneration and further progress to endometritis and other reproductive disorders. Photodynamic therapy (PDT) is a promising bacterial strategy in the postantibiotic era. However, its therapeutic efficacy is often limited by the rapid recombination of photogenerated charge carriers and the limited penetration depth of visible excitation light. In this study, we report a previously unexplored up-conversion-mediated, Type I dominant photodynamic antibacterial uterine scaffold specifically designed for infection-associated endometrial injury. The core innovation lies in the construction of a Schottky Ag 0 -Ag 2 S heterojunction on the surface of NaYF 4 :Yb,Tm nanoparticles using ZIF-8 as a sacrificial precursor, which enabled highly efficient charge separation and oxygen-independent hydroxyl radical generation, effectively overcoming the inherent oxygen dependence and compromised performance of conventional Type II PDT under hypoxic uterine conditions. The upcon-version core further enables deep-tissue-penetrable NIR activation, addressing the poor penetration of visible light. Moreover, beyond instantaneous PDT, the dynamic release of Ag + ions provides synergistic bactericidal activity, enabling spatiotemporally coordinated biofilm disruption. Importantly, these nanostructures were incorporated into patient-customizable, biodegradable PLLA scaffolds fabricated by selective laser sintering, achieving simultaneous antibacterial therapy and endometrial regeneration in a single platform. Collectively, the integration of an oxygen-independent PDT mechanism, MOF-templated heterojunction engineering, and 3D printed personalized uterine implants constitutes a comprehensive therapeutic strategy not previously reported for treating infection and endometrial injury. The alternative text for this image may have been generated using AI.
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CARDIOVASCULAR DRUGS AND THERAPY
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2026
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IF 3.5
Chen XinJing,Hong Jingxuan
Objective This study aimed to determine whether dexmedetomidine (DEX) attenuates myocardial ischemia/reperfusion injury (MIRI) by regulating DNA methyltransferase 3B (DNMT3B)–mediated methylation of a CpG island within the Kruppel-like factor 4 (KLF4) promoter. Methods A rat model of MIRI was established and treated with DEX, DNMT3B knockdown, or DEX combined with DNMT3B overexpression. Histopathological alterations, apoptosis, and DNMT3B/KLF4 expression were assessed using H&E staining, TUNEL/cTnT co-staining, RT-qPCR, western blot, immunofluorescence, and immunohistochemistry. H9C2 cardiomyocytes subjected to oxygen-glucose deprivation/reperfusion (OGD/R) were treated with DEX and transfected with DNMT3B or KLF4 knockdown/overexpression plasmids. CpG island methylation within the KLF4 promoter was predicted using MSP and ChIP-PCR. Results MIRI impaired cardiac function, increased infarct size and myocardial enzyme release, upregulated DNMT3B expression, downregulated KLF4 expression, and promoted cardiomyocyte apoptosis. DNMT3B was predominantly localized in cTnT-positive cardiomyocytes. DEX treatment or DNMT3B knockdown reduced DNMT3B expression, restored KLF4 levels, and alleviated myocardial injury in vivo as well as OGD/R-induced damage in vitro . DEX was associated with decreased methylation of the investigated CpG island within the KLF4 promoter. DEX may inhibit DNMT3B-mediated promoter hypermethylation, thereby enhancing KLF4 transcription. KLF4 knockdown abolished the cardioprotective effects of DEX, while DNMT3B overexpression reversed DEX-mediated improvements in vivo . Conclusions DEX may attenuate MI/RI by downregulating DNMT3B and reducing methylation at a CpG island within the KLF4 promoter, thereby promoting KLF4 expression. However, the intrinsic resolution limitations of MSP and ChIP-PCR restrict precise mapping of methylation sites. Higher-resolution techniques, such as whole-genome bisulfite sequencing or single-molecule methylation sequencing, are warranted to validate these findings.
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JOURNAL OF MOLECULAR HISTOLOGY
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2026
·Vol. 57
·期 3
·142
·
IF 2.6
Ye Chunhua,Liu Hua,Ming Zhu,Yi Qinjun,Xiang Rong,Chen Yifu
This study investigated the role of TNF receptor associated factor 6 (TRAF6) in glomerular podocyte pyroptosis. An in vitro LN cell model was established by treating mouse glomerular podocyte MPC5 cells with 100 ng/ml LPS for 12 h. Concurrently, LN cells were treated with TRAF6 knockdown and TXNIP overexpression or NLRP3 activation (Nigericin). MRL/lpr mice were treated with adenovirus vector expressing sh-TRAF6 for in vivo validation. LPS treatment decreased cell viability, increased cell death, elevated Desmin mRNA, malondialdehyde, TRAF6, TXNIP, interleukin (IL)-1β, and IL-18, reduced Nephrin and Podocin mRNA (podocyte markers), superoxide dismutase, and glutathione levels, enhanced cell pyroptosis and lactate dehydrogenase concentration, and activated the NLRP3 inflammasome pathway. TRAF6 knockdown suppressed LPS-stimulated podocyte damage, oxidative stress, and pyroptosis. TRAF6 interacted with TXNIP at the protein level, and TRAF6 increased TXNIP protein expression. The protective functions of TRAF6 silencing on MPC5 cells were counteracted upon TXNIP overexpression or NLRP3 activation. In vivo, TRAF6 knockdown regulated the TXNIP/NLRP3 inflammasome pathway to alleviate renal tissue pathological changes and fibrosis in LN mice. In conclusion, TRAF6 activates the NLRP3 inflammasome pathway through regulating TXNIP, thereby accelerating LPS-induced oxidative stress, pyroptosis, and damage to glomerular podocytes. Hence, TRAF6 may be a promising therapeutic target for LN. Graphical abstract The alternative text for this image may have been generated using AI.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Tan Renjie,Zhang Ke,Si Yifan,Huang Kaisong,Jia Weibin,Li Min,Iqbal Saira,Zhang Shuai,Zhai Zhenya,Hu Jinlian
Peripheral nerve injury (PNI) often leads to limited functional recovery in over 50% of patients, with current clinical strategies primarily relying on nerve grafts or conduits to bridge injury gaps. In the search of alternative solutions, self-powered biomaterials show significant potential in tissue engineering applications. This study designed a self-powered polycaprolactone/gelatin (PCL/gelatin) nanofiber conduit for sciatic nerve regeneration. The electrospun PCL/gelatin nanofiber conduit was characterized for its self-powering capability, mechanical strength, biocompatibility, and degradability. Functionalization of the nanofiber conduit with conductive polypyrrole (PPy) nanoparticles loaded with nonsteroidal anti-inflammatory drugs (NSAIDs) enhanced anti-inflammatory and antioxidative effects and helped prevent muscle atrophy. Furthermore, the incorporation of conductive polymers reduced polarization cancellation and enabled the controlled release of NSAIDs through exercise-induced mechanical stimulation of the nanofiber conduit. Transcriptomic analyses confirmed that the nanofiber conduit promoted peripheral nerve regeneration and inhibited apoptosis by activating insulin signaling. Graphical
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Zhao Haojie,Han Fei,Sun Xinyue,Gao Zexi,Zheng Zeyan,Niu Yajing,Li Wenping,Su Chao,Li Zhonghong,Tang Wenzhi
The timely and cost-effective detection of infectious pathogens is critical for their prevention and control. In this study, a low-cost, portable, and user-friendly WiFi-enabled Internet of Things (IoT) monitoring system was developed for the detection of infectious pathogens. The detection mechanism relies on real-time monitoring of hydroxy naphthol blue (HNB) colorimetric signals, which are triggered by the depletion of Mg 2+ ions during loop-mediated isothermal amplification (LAMP). A 3D-printed, portable reader was developed for both LAMP incubation and real-time HNB signal monitoring. Analysis at an incubation temperature of 65 °C demonstrated the excellent thermal stability of HNB and its linear correlation ( R 2 = 0.9973) with Mg 2+ ion consumption, highlighting its suitability as a real-time indicator. The detection capability was validated using the Nosema bombycis SSU rRNA gene and the Bombyx mori nucleopolyhedrovirus gp41 gene as model targets. The results showed linear relationships ( R 2 > 0.99) between threshold time and log target concentration, with the limit of detection (LOD) reaching 1 copy µL −1 for both targets. Analysis of Nosema bombycis in real samples showed comparable performance to the conventional female moth microscopy method. Furthermore, the IoT-enabled reader allowed for fully automated result reporting via email, facilitating timely implementation of containment measures. This study reported on the development of a portable and user-friendly IoT-based test platform, presenting attractive potential for point-of-care diagnosis in infectious diseases, particularly in resource-constrained settings. Graphical
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2026
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IF 4.7
Quan Maohua,Liu Junchao,Wang Jingxia
Solvent-responsive photonic films with programmable deformation are attractive for smart indicators and reconfigurable devices. Here, we present a Janus poly(vinylidene fluoride-cohexafluoropropylene) (PVDF-HFP) inverse opal film composed of a nanoporous upper layer and a dense lower layer, enabling directional bending, solvent-induced plastic deformation with residual-stress-driven reversible configuration change. Exposure to 1,2-dichloroethane induces asymmetric infiltration of the nanostructured pores, generating capillary pressure sufficient to plastically deform the porous layer and produce a characteristic two-step bending behavior. The bending amplitude and accompanying photonic bandgap shift (580–710 nm) are tunable through pore size, solvent volume, and application position. Mechanical and structural analyses support a capillarity-driven plastic deformation mechanism linking nanoscale architecture to macroscopic actuation. The resulting photonic Janus film offers a simple, visually interpretable platform for solvent-triggered shape morphing and exposure indication.
The alternative text for this image may have been generated using AI.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2026
·Vol. 252
·期 5
·197
·
IF 4.2
Shi Zhenbao,Yang Shaojie,Jiang Mingjun,Zhang Jinglong,Wu Dianhui
Fermented green plum wine (GPW) is a typical high-acid fruit wine in which nitrogen source regulation plays an important role in yeast metabolism and flavor formation. However, the effects of peptide-based nitrogen sources in such systems remain insufficiently understood. This study systematically compared the effects of six plant-derived protein peptides on fermentation performance and flavor formation in GPW. Corn protein peptides showed the most pronounced effect, reducing fermentation time from 18 days to 11–12 days and enhancing ethanol production efficiency. A total of 103 volatile compounds were identified, with esters and higher alcohols accounting for 76–84% of total volatiles. Corn peptides promoted the accumulation of fruity and floral esters and aromatic higher alcohols. Ethyl octanoate reached 1052.40 µg·L⁻¹, approximately twice that of the control. Relative odor activity value (ROAV) analysis identified six key aroma-active compounds (ROAV ≥ 1.0), with β-damascenone and β-ionone contributing prominently in the corn peptide group. Untargeted metabolomics indicated enrichment of fatty acid degradation and L-phenylalanine metabolism, suggesting enhanced aroma precursor formation. These findings provide experimental evidence for flavor optimization of high-acid fruit wine through nitrogen source regulation.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
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2026
·Vol. 41
·期 2
·285-295
·
IF 1.4
Li Yaohu,Lou Wenbo,Pan Hongfei
We successfully incorporated phenyl groups into a small-molecule quaternary ammonium cross-linker and synthesized cross-linked polybenzimidazole membranes via a one-step cross-linking process. Compared with conventional quaternary ammonium-crosslinked benzimidazole membranes, the introduction of phenyl groups significantly increases the free volume within the membrane. After phosphoric acid doping, the benzimidazole membrane with larger free volume retains more phosphoric acid compared to conventional quaternary ammonium-crosslinked membranes, forming an extensive hydrogen-bonding network that effectively enhances its anhydrous proton conductivity. The anhydrous proton conductivity reaches 91 mS·cm −1 at 160 °C, substantially higher than that of conventional quaternary ammonium-crosslinked membranes with the same mass fraction. Benefiting from the improved conductivity, the membrane electrode assembly exhibits reduced ohmic polarization, achieving a peak power density of 792 mW·cm −2 at 160 °C.
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JOURNAL OF POLYMER RESEARCH
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2026
·Vol. 33
·期 5
·179
·
IF 3.5
Wang Jie,Meng Junqing,Lyu Chunhui,Lyu Yingpei
Traditional fire prevention technologies have primarily aimed at enhancing flame-retardant performance, often neglecting the integrated improvement of flow properties. A superhydrophobic coated flame-retardant fabric was developed, and a flow simulation test system was established to systematically investigate the effects of hydrophobic surfaces, polymers (polyethylene oxide, PEO, and guar gum, GG), and a surfactant (dodecyl hydroxy sulfobetaine, DHSB) on fluid flow behavior. The influence of the coating on flame retardancy and the effect of polymer-surfactant additives on the thermal stability of wood were also evaluated. Through comprehensive improvement rate assessment and Pearson correlation analysis, a highly consistent integrated trend was identified between the enhanced flow efficiency and the improved flame retardancy conferred by the superhydrophobic coating and the PEO/GG + DHSB composite systems, validating the effective integrated optimization within the designed formulations. The surfactant DHSB was found to optimize the polymer’s function within the fluid boundary layer and contributed to flame retardation through multiple mechanisms during pyrolysis, including gas dilution, char formation catalysis, and free radical quenching. Based on quantitative parameters such as the comprehensive improvement rate and correlation analysis, this study provides a clear basis for material design and additive optimization in various fire prevention application scenarios.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
·
IF 4.1
Feng Rui,Wang Yupeng,Chen Yonghua,Li Tianxu,Zhou Houfeng,Zhan Zhenye,He Yuying,Tang Xialin,Su Jianfen,Guo Yuhuai,Chen Xiaomin,Zan Jie
Traumatic brain injury (TBI) represents a significant global health challenge requiring rapid and accurate diagnostic tools. S100 calcium-binding protein B (S100B) serves as a consensus-endorsed serum biomarker for TBI, but current detection methods lack the speed and simplicity needed for on-site clinical applications. This study reports the development of a time-resolved fluorescence immunochromatographic test strip (TRFIS) for rapid and quantitative serum S100B detection. High-affinity monoclonal antibodies against S100B were generated via hybridoma technology, and time-resolved fluorescent microspheres (TRFMs) were integrated with lateral flow immunoassay. The TRFIS demonstrated excellent analytical performance with a linear range of 15.6–1000 pg/mL ( R 2 = 0.9885), a detection limit of 12.6 pg/mL, and a test time of approximately 13 min. Intra- and inter-batch coefficients of variation were both < 10.0%, confirming high reproducibility. This platform offers high sensitivity, accuracy, and simplicity, making it potentially suitable for on-site TBI diagnosis in resource-limited healthcare settings. Graphical abstract
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产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2026
·Vol. 9
·期 1
·91
·
IF 21.8
Fang Xuan,Zhao Hongqi,Ke Wentao,Zou Yi,Xu Tao,Chen Guangzi,Li Jian,Sheng Gaohong,Cheng Haozhe,Zhu Hao,Xiao Jun,Toh Wei Seong,Lin Sien,Wang Tianqi,Ma Tian
Osteoarthritis (OA) is a chronic joint disorder characterized by synovial inflammation, cartilage degradation and macrophage polarization imbalance, where excessive reactive oxygen species (ROS) accumulation and mitochondrial dysfunction exacerbate pro-inflammatory M1 macrophage polarization. Here, a pH-responsive nanocomposite (Se/LDH@PEG-M) is developed by integrating selenium (Se) within MgAl-layered double hydroxide (MgAl-LDH), modified with PEGylation and loaded with the mitochondria-targeted antioxidant Mito-TEMPO. The synthesized nanocomposite retains glutathione peroxidase (GPX)-like activity and the antioxidant capacity of Se nanoparticles, while exhibiting pH-responsive degradation to neutralize acidic OA microenvironments and release Mg²⁺, Se and Mito-TEMPO for inflammation suppression, ROS scavenging and macrophage polarization modulation. In vitro, Se/LDH@PEG-M effectively inhibits pro-inflammatory M1 polarization while promoting anti-inflammatory M2 phenotypes by suppressing multiple inflammatory pathways, restoring redox homeostasis, and enhancing mitochondrial oxidative phosphorylation and ATP production via respiratory chain activation. Conditioned medium from Se/LDH@PEG-M-treated macrophages attenuates chondrocyte apoptosis, inflammation and catabolism compared to LPS group. In murine OA models, intra-articular Se/LDH@PEG-M administration effectively alleviates synovial inflammation, suppresses osteophyte formation and mitigates cartilage degradation. This multifunctional nanocomposite concurrently targets OA pathogenesis through macrophage immunomodulation, ROS scavenging and mitochondrial protection, providing a potential therapeutic strategy for OA management. Graphical Schematic illustration of the synthesis process and therapeutic mechanism of Se/LDH@PEG-M. ( A )The synthesis procedure of Se/LDH@PEG-M nanocomposites. The nanocomposites were developed by integrating selenium (Se) into Mg-Al layered double hydroxides, followed by PEG modification and loading with mitochondria-targeted antioxidant Mito-TEMPO. ( B ) The therapeutic mechanism of Se/LDH@PEG-M. The nanocomposites degraded in acidic OA microenvironments and releasing Mg²⁺, Se and Mito-TEMPO for inflammation suppression, ROS scavenging and immunomodulation.
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