MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Lingsong Lu,Bei Liu
3D porous Fe 3 O 4 @ZIF-90 interface provides high-density DNA loading sites. • Mn 2+ -gated DNAzyme walker ensures a low-background controllable assay. • DNAzyme walker and CRISPR-Cas13a cascade enable dual signal amplification. • The biosensor demonstrated high accuracy and specificity in human serum samples. The precise quantification of Mucin-1 (MUC1) is vital for the early diagnosis and therapeutic monitoring of epithelial malignancies. In this study, a fluorescent biosensing platform based on a 3D porous metal-organic framework (MOF) and a cascaded signal amplification strategy was developed for MUC1 detection, driven by a target-induced conformational switch and Mn 2+ -dependent catalytic DNAzyme walking. Porous Fe 3 O 4 @ZIF-90 nanocomposites were used to construct the sensing interface, which co-immobilized dormant DNAzyme/aptamer duplexes and a high-density layer of substrate DNA (sub-DNA), serving as a robust platform for the subsequent walking process. Upon specific MUC1 recognition, the aptamer preferentially captures the target MUC1. The thermodynamic driving force induces the dissociation of the MUC1/aptamer complex, liberating the DNAzyme engine. Following magnetic separation to eliminate matrix interferences, the addition of Mn 2+ triggers the processive inter-molecular migration of the DNAzyme walker across the sub-DNA immobilized 3D porous Fe 3 O 4 @ZIF-90 interface via successive hybridization and site-specific cleavage of internal riboadenosine (rA) junctions. This inter-molecular walking process generates a high flux of trigger strands, which subsequently activates the CRISPR-Cas13a system for a robust trans -cleavage of dual-labeled fluorophore-quencher (FQ) reporters. The resulting degradation of FQ reporter enables sensitive MUC1 quantification through exponential fluorescence recovery. By synergizing the structure advantages of the 3D MOF with this cascaded signal amplification, the biosensor achieved a wide linear range from 0.37 nM to 500 nM with a low detection limit of 17.2 pM. Furthermore, the biosensor exhibited reliable specificity and recovery (102.28%–106.94%) in complex human serum assays. The proposed strategy effectively minimizes non-specific background leakage, demonstrating promising potential for the ultra-sensitive detection of MUC1 in complex biological matrices. Schematic presentation of the fluorescent biosensor for the determination of Mucin-1 based on a 3D porous Fe 3 O 4 @ZIF-90 interface and DNAzyme walker-mediated CRISPR-Cas13a cascade. Download: Download high-res image (117KB) Download: Download full-size image
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高影响因子
POLYMER BULLETIN
·
2016
·Vol. 74
·期 1
·75-90
·
IF 1.371
Chen Xiuqiong,Yan Huiqiong,Shi Zaifeng,Feng Yuhong,Li Jiacheng,Lin Qiang,Wang Xianghui,Sun Wei
A novel biosensor based on electro-co-deposition of myoglobin (Mb), sodium alginate (SA), Fe 3 O 4 -graphene (Fe 3 O 4 -GR) composite on the carbon ionic liquid electrode (CILE) was fabricated using Nafion as the film forming material to improve the stability of protein immobilized on the electrode surface, and the modified electrode was abbreviated as Nafion/Mb-SA-Fe 3 O 4 -GR/CILE. FT-IR and UV–vis absorption spectra suggested that Mb could retain its native structure after being immobilized in the SA-Fe 3 O 4 -GR composite film. The electrochemical behavior of the modified electrode was studied by cyclic voltammetry, and a pair of symmetric redox peaks appeared in the cyclic voltammograms, indicating that direct electron transfer of Mb was realized on the modified electrode, which was ascribed to the good electrocatalytic capability of Fe 3 O 4 -GR composite, the good biocompatibility of SA and the synergistic effects of SA and Fe 3 O 4 -GR composite. The electrochemical parameters of the electron transfer number ( n ), the charge transfer coefficient ( α ) and the electron transfer rate constant ( k
s ) were calculated as 0.982, 0.357 and 0.234 s −1 , respectively. The modified electrode exhibited good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) with wide linear range from 1.4 to 119.4 mmol/L, low detection limit as 0.174 mmol/L (3 σ ), good stability and reproducibility.
研究领域:
生物传感器
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电化学
·
蛋白质电化学
·
纳米材料
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生物相容性材料
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分子诊断
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转化医学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 91
·74548-74556
·
IF 3.84
Jianfeng Fei,Wenchao Dou,Guangying Zhao
A rapid and highly sensitive sandwich electrochemical immunoassay method was constructed for Salmonella pullorum and Salmonella gallinarum (S. pullorum and S. gallinarum) determination based on immune magnetic beads (MBs) and an enzyme labeled antibody. An abundance of gold nanoparticles (AuNPs) were attached to SiO2 coated Fe3O4 nanoparticles (Fe3O4/SiO2) via covalent binding between the –SH groups of Fe3O4/SiO2 and the AuNPs. Antibodies against S. pullorum and S. gallinarum were immobilized on Fe3O4/SiO2/AuNPs nanocomposites (AuMNPs) by automatic adsorption between thiol and the AuNPs. S. pullorum and S. gallinarum in the sample were captured by the AuMNPs and separated from the samples by applying an external magnetic field. The AuMNPs–Salmonella complexes (Ag/Ab1/AuMNPs) were re-dispersed in a buffer solution then exposed to horseradish peroxidase labeled anti-S. pullorum and S. gallinarum (HRP-Ab2) solution, forming a sandwich-type immune complex (HRP-Ab2/Ag/Ab1/AuMNPs). A 4 channel screen printed carbon electrode (4-SPCE) was modified by gold nanoparticles (AuNPs) through the electrodeposition method to prepare AuNPs/4-SPCE. After magnetically separating the sandwich immune complexes from solution, HRP-Ab2/Ag/Ab1/AuMNPs was anchored on AuNPs/4-SPCE by a magnet. A linear response to S. pullorum and S. gallinarum was obtained in the concentration range from 102 to 106 CFU mL−1, with a limit of detection of 3.2 × 101 CFU mL−1 (at an SNR of 3). This nanoparticle-based immunoassay method offers sensitive, highly specific, and reproducible detection of S. pullorum and S. gallinarum. Given its low detection limit, it represents promising potential in the detection of other food-borne pathogens by exchanging the antibody.
研究领域:
生物传感器
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食品安全
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电分析化学
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纳米生物技术
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微生物检测
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ying Wu,Chi Song,Xinyan Yu,Xianbao Shen,Lijie Xu,Ying Zhang,Han Gong,Changlei Xia,Lu Gan
In this study, waste polyethylene (PP) obtained from obsoleting water purifier filter was mixed with ferric chloride hexahydrate (FeCl 3 ·6H 2 O) to prepare a series of single crystal phased iron compound incorporated carbon composites (Fe/PP-C). The prepared Fe/PP-Cx catalysts were then utilized to degrade sulfamethoxazole (SMX) in water by activating peroxymonosulfate (PMS). The results showed that with the assistance of pyrolysis temperature regulation, waste PP filter could control the crystalline phase of the iron compound in the prepared Fe/PP-Cx through releasing distinct gaseous atmospheres at respective pyrolysis temperature. When the pyrolysis temperature was set at 600 °C and 900 °C, pure phase ferric oxide (Fe 3 O 4 ) and zero-valent iron (ZVI) incorporated carbon catalysts were obtained, respectively. 0.2 g/L of Fe/PP-Cx could completely degrade 0.02 mM of SMX within 20 min, in which the toxicity of the reaction solution was also effectively reduced. In both Fe/PP-Cx/PMS systems, non-radical mechanisms dominated the SMX degradation, in which singlet oxygen played the dominant role in the Fe/PP-C600/PMS system, whereas electron transfer process was primarily responsible for SMX degradation in the Fe/PP-C900/PMS system. High-valent iron-oxo species was also involved in SMX degradation in the Fe/PP-C900/PMS system. Since Fe/PP-C600 and Fe/PP-C900 respectively activated PMS via heterogeneous and homogeneous routes, Fe/PP-C600 maintained higher longtime use stability. This study aims to present a simple approach to manipulate the nonradical organic pollutant degradation mechanisms in PMS activated system, which also provides a new strategy of utilizing waste polymer.
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高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Li Yang,Shuang Wang,Zhongshui Xie,Rongrong Xing,Runqin Wang,Xuan Chen,Shuang Hu
A novel deep eutectic solvent - loaded magnetic solid phase extraction (DES-L-MSPE) method was proposed, which was based on the direct loading of a hydrophobic DES composed of L-menthol and lactic acid (1:2) into the pores of core–shell magnetic Fe 3 O 4 @MIL-101(Cr) nanoparticles. The function of DES was to condition the Fe 3 O 4 @MIL-101(Cr) and promote liquid phase microextraction for the analytes. Through coupling with HPLC-UV analysis, this method was utilized for the determination of six non-steroidal anti-inflammatory drugs (NSAIDs), including ketoprofen, naproxen, flurbiprofen, diclofenac sodium, ibuprofen, and mefenamic acid in environmental water samples. The influencing parameters such as type and ratio of DES, amount of adsorbent, sample solution pH, salt concentration, extraction time, elution solvent and time were investigated and optimized in detail. Under the optimal conditions, the method exhibited good linearity (5.0–1000 μg/L for ketoprofen, ibuprofen, 1.5–600 μg/L for flurbiprofen, mefenamic acid, 2.5–1000 μg/L for naproxen and 3.0–600 μg/L for diclofenac sodium with the correlation coefficients ranging from 0.9972 to 0.9993), low limits of detection (0.20–1.1 μg/L), and high precision (RSD < 8.2 %). Besides, the recoveries in spiked lake and river water samples were 89.8 %–110.4 % and 83.8 %–109.0 %, respectively. Taken together, the established method was proved to be simple, fast, highly sensitive and accurate for the determination of NSAIDs from aqueous matrices.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Deng Ze-Hui,Wang Xia,Wang Xiao-Li,Gao Cui-Ling,Dong Liang,Wang Ming-Lin,Zhao Ru-Song
A magnetic covalent organic framework (Fe 3 O 4 @COF) with core–shell structure was fabricated at room temperature and used as an adsorbent for magnetic solid-phase extraction of polar endocrine-disrupting phenols (4-n-nonylphenol, 4-n-octylphenol, bisphenol A and bisphenol AF). The sorbent was characterized by transmission electron microscopy, FTIR, powder X-ray diffraction and other techniques. The main parameters governing the extraction efficiency were optimized. The phenols were quantified by HPLC with fluorometric detection. The method has attractive features such as low limits of detection (0.08–0.21 ng.mL −1 ), wide linear ranges (0.5–1000 ng.mL −1 ), and good repeatability (intra-day: 0.39%–4.99%; inter-day: 1.57%–5.21%). Satisfactory results were obtained when the developed method was applied to determine the four target pollutants in real world drink samples with spiked recoveries over the range of 81.3~118.0%. This indicates that the method is a powerful tool for the enrichment and determination of endocrine-disrupting phenols in drink samples.
A magnetite based covalent organic framework (Fe 3 O 4 @COFs) was synthesized with TPAB, TPA and Fe 3 O 4 . It was used for magnetic solid-phase extraction of endocrine-disrupting phenols from plastic-packaged tea drink samples coupled with liquid chromatography (LC) for determination.
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PubMed
高影响因子
Journal of Applied Spectroscopy
·
2019
·Vol. 86
·期 1
·89-95
·
IF 0.675
Wang Haojiang,Wang Xuan,Bian Wei,Sun Tijian,Cai Zongwei,Wei Jiancong
Core-shell magnetic metal organic nanocrystals were synthesized and used as matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four nitropolycyclic aromatic hydrocarbons (nitro-PAHs) were successfully detected by using negative-ion MALDI-TOF MS without background interference. Furthermore, the magnetic nanocomposites (MNCs) coated with zeolitic imidazolate framework-8 (ZIF-8) showed excellent adsorption and enrichment capacity, and can be isolated with a magnet and directly spotted on the stainless steel plate for MALDI measurement. It was also analyzed nitro-PAHs in PM2.5 samples using the ZIF-8-coated magnetic nanocomposite as adsorbent and matrix.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 32
·12563-12569
·
IF 3.069
Huixiao Duo,Xiaofeng Lu,Shuai Wang,Licheng Wang,Yong Guo,Xiaojing Liang
Herein, a novel, fusiform-like magnetic metal–organic framework material (Fe3O4-NH2@MOF-235) was fabricated by a facile two-step solvothermal approach. Then, Fe3O4-NH2@MOF-235 was applied as an adsorbent in magnetic solid-phase extraction (MSPE) coupled with high-performance liquid chromatography (HPLC) for the simultaneous extraction and determination of five kinds of benzoylurea insecticides (flufenoxuron, chlorbenzuron, teflubenzuron, diflubenzuron and triflumuron) from honey, fruit juice and tap water samples. Moreover, potential variables influencing the MSPE efficiency were optimized. Under the optimized conditions, an excellent linearity was achieved in the range of 1.0–300.0 μg L−1 for teflubenzuron and flufenoxuron and 2.0–300.0 μg L−1 for diflubenzuron, chlorbenzuron and triflumuron, with the correlation coefficients of 0.9991–0.9995. The limits of detection for the analytes were 0.25–0.5 μg L−1, and the recoveries at three fortified concentration levels ranged from 76.45% to 90.74% for the honey samples, 79.95–95.27% for the fruit juice samples, and 84.02–99.62% for the tap water samples. The π–π stacking and hydrophobic interactions between Fe3O4-NH2@MOF-235 and the analytes played an important role in the adsorption process. Moreover, maximum adsorption could be achieved within 5 minutes with very little adsorbent for all the samples. Therefore, the magnetic Fe3O4-NH2@MOF-235 composite combined with MSPE is a convenient approach to detect benzoylurea insecticides in actual samples.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Fukai Li,Aijun Gong,Lina Qiu,Weiwei Zhang,Jingrui Li,Zhe Liu
In this work, magnetic nanomaterials were synthesized by grafting diglycolamide (DGA) derivatives onto polydopamine (PDA) functionalized magnetic nanomaterials (Fe 3 O 4 @PDA@DGA). The adsorption behaviors of Fe 3 O 4 @PDA@DGA for rare earth elements (REEs) were evaluated. The characterization results indicated that the Fe 3 O 4 nanoparticles form a hexagonal-like two-dimensional projection shape, and DGA is successfully grafted onto the surface of the nanomaterials. By using Fe 3 O 4 @PDA@DGA nanomaterials as the adsorption material, a novel method for magnetic solid phase extraction (MSPE) coupled with inductively coupled plasma optical emission spectrometry (ICP-OES) was developed, which can quantitatively determine trace REEs in environmental water. With the MSPE procedure, the parameters affecting the adsorption behaviors of the REEs on Fe 3 O 4 @PDA@DGA nanomaterials, such as pH, ultrasonic adsorption time, mass and solution volume were investigated systematically. Under the optimum experimental conditions, the limits of detections (LODs) and the relative standard deviations (RSDs) of 16 REEs (C = 10 µg L −1 , n = 7) were in the range of 0.039 µg L −1 for Eu to 0.425 µg L −1 for Nd and 1% for La to 3.9% for Gd. The linear range of the calibration curve over 2–1000 ng mL −1 (R 2 > 0.999) was obtained for the target analytes. This developed method was applied for the environmental samples and the recoveries were 95.8–105.8% for the spiked water. The proposed MSPE-ICP-OES method has the advantages of easy separation, simple operation, good anti-interference capability, fast adsorption and elution kinetics, low detection limits, high sensitivity and fast analysis speed, thereby acting as a potential application for the simultaneous analysis of trace REEs in environmental samples.
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高影响因子
Polymers
·
2018
·Vol. 10
·期 12
·1329
·
IF 2.935
Lingyu Liu,Xudong Zhu,Yanbo Zeng,Hailong Wang,Yixia Lu,Jian Zhang,Zhengzhi Yin,Zhidong Chen,Yiwen Yang,Lei Li
In this paper, we report a new composite of reduced graphene oxide/Fe3O4-ionic liquid based molecularly imprinted polymer (RGO/Fe3O4-IL-MIP) fabricated for diphenylamine (DPA) detection. RGO/Fe3O4-IL-MIP was prepared with RGO/Fe3O4as supporter, ionic liquid 1-vinyl-3-butylimidazolium hexafluorophosphate ([VC4mim][PF6]) as functional monomer, ionic liquid 1,4-butanediyl-3,3’-bis-l-vinylimidazolium dihexafluorophosphate ([V2C4(mim)2][(PF6)2]) as cross-linker, and diphenylamine (DPA) as template molecule. Fourier transform infrared spectroscopy, thermal gravimetric analysis, scanning electron microscopy, and vibrating sample magnetometer were employed to characterize the RGO/Fe3O4-IL-MIP composite. RGO/Fe3O4-IL-MIP was then drop-cast onto a glassy carbon electrode to construct an electrochemical sensor for DPA. The differential pulse voltammetry (DPV) peak current response for 20 μM DPA of RGO/Fe3O4-IL-MIP modified glassy carbon electrode (GCE) was 3.24 and 1.68 times that of RGO/Fe3O4-IL-NIP and RGO/Fe3O4-EGDMA-MIP modified GCEs, respectively, indicating the advantage of RGO/Fe3O4-IL-MIP based on ionic liquid (IL) as a cross-linker. The RGO/Fe3O4-IL-MIP sensor demonstrated good recognition for DPA. Under the optimized conditions, the RGO/Fe3O4-IL-MIP sensor exhibited a DPA detection limit of 0.05 μM (S/N = 3) with a linear range of 0.1–30 μM. Moreover, the new RGO/Fe3O4-IL-MIP based sensor detected DPA in real samples with satisfactory results.Keywords:reduced graphene oxide;Fe3O4;molecularly imprinted polymer;ionic liquid cross-linker;electrochemical sensor;diphenylamine
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 4.321
Jie-Ping Fan,Xiao-Kang Xu,Rui Xu,Xue-Hong Zhang,Jian-Hang Zhu
In this work, the superparamagnetic Fe 3 O 4 particles prepared by the coprecipitation method were coated successively by SiO 2 , vinyl end groups and molecularly imprinted polymer (MIP) shells to afford a pomegranate-like core/shell superparamagnetic particle (Fe 3 O 4 @SiO 2 @MIP), and the MIP shell preparation conditions were optimized by multi-objective response surface methodology. In order to simultaneously enrich and separate the four taxoids (10-DAB, baccatin III, cephalomannine and paclitaxel), cephalomannine was selected as the template because of its similar chemical structure to other three taxoids and relative inexpensiveness. Especially, to avoid template leaking which may influence the accuracy of identification and quantitation of the analytes, we selected paclitaxel as the target molecular during the investigation of the adsorption property of Fe 3 O 4 @SiO 2 @MIP particles. This strategy was similar to the dummy molecular imprinting technique in which the dummy molecularly imprinted polymer was prepared by using a dummy template, structural analog of the target analyte. The as-prepared Fe 3 O 4 @SiO 2 @MIP particles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FTIR). The adsorption kinetics, isotherms and reusability were also investigated. Finally, the Fe 3 O 4 @SiO 2 @MIP particles were successfully applied to simultaneously enrich and separate these four taxoids in the extract of Taxus × media ( T. × media ).
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高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Shaoming Fang,Xiaodong Dong,Shunli Liu,Donglai Penng,Linghao He,Minghua Wang,Guodong Fu,Xiaozhong Feng,Zhihong Zhang
In this paper, we report a novel label-free electrochemical aptasensor for detecting proteins in whole blood based on a three-component nanocomposite , in which ferriferrous oxide and three-dimensional graphene nanocomposite were modified with the plasma-polymerized 4-vinyl pyridine (Fe 3 O 4 @3D-rGO@PP4VP). In this novel sensing strategy, large amounts of amino groups in PP4VP facilitated the immobilization of aptamer strands via the strong electrostatic interaction between positively charged ammonium groups of the nanocomposites and negatively charged phosphate groups of aptamers. In the presence of thrombin, LYS (LYS), and platelet-derived growth factor-BB (PDGF-BB), the adsorbed aptamer strands on the developed nanocomposite surface caught the targeted proteins at the electrode interface. The aptamer preferred to be a barrier for electrons and inhibited electron transfer, leading to the decreased peak current of cyclic voltammetry measurements and the increased electron transfer resistance of electrochemical impedance spectroscopy . The determination of the thrombin, PDGF-BB, and LYS concentrations with this novel strategy showed low detection limits of 4.5, 29.4, and 14 pg·mL −1 , and the analytical ranges extend from 0.01 to 50, 0.1 to 100, and 0.1 to 200 ng·mL −1 , respectively. The resultant aptasensor exhibited high selectivity, acceptable reproducibility, and stability toward thrombin. The aptasensor could be used to detect thrombin in whole blood samples, thereby suggesting its possible application in clinical settings.
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高影响因子
危险品化学品经营许可证（带存储）