JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-22
·
IF 12.6
Yang Ningning,Hua Rongrong,Lai Yingying,Zhu Peijun,Ding Jian,Ma Xianhui,Yu Gaoxiang,Xia Yiheng,Liang Chao,Gao Weiyang,Wang Zhouguang,Zhang Hongyu,Yang Liangliang,Zhou Kailiang,Ge Lu
In the field of large-area trauma flap transplantation, preventing avascular necrosis remains a critical challenge. Key mechanisms for improving flap viability include angiogenesis promotion, oxidative stress inhibition, and cell death prevention. Recently, two-dimensional ultrathin Ti 3 C 2 T X (MXene) nanosheets have gained attention for their potential contributions to these processes, though MXene’s physiological impact on flap survival had not been previously investigated. This study is the first to confirm MXene's biological effects on the ischaemic microenvironment post-skin flap transplantation. Findings indicated that MXene significantly decreased the necrotic area in ischaemic flaps (37.96% ± 2.00%), with reductions of 30.40% ± 1.86% at 1 mg/mL and 20.19% ± 2.11% at 2 mg/mL in a concentration-dependent manner. Mechanistically, MXene facilitated in situ angiogenesis, mitigated oxidative stress, suppressed pro-inflammatory pyroptosis, and activated the PI3K-Akt pathway, particularly influencing vascular endothelial cells. Comparative transcriptome analysis of skin tissues with and without MXene treatment provided additional evidence, highlighting mechanisms such as pro-inflammatory pyroptosis, ROS metabolic processes, endothelial cell proliferation regulation, and PI3K-Akt signaling pathway activation. Overall, MXene demonstrated biological activity, effectively promoting ischaemic flaps survival and presenting a novel strategy for addressing ischaemic necrosis in skin flaps. Graphical abstract
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ARCHIVES OF DERMATOLOGICAL RESEARCH
·
2025
·Vol. 317
·期 1
·1-16
·
IF 2.1
Lin Ling,Li Wei,Gao Xinjing,Li Qian,Zhou Xin,Liu Weiyu,Zhong Xuelian,Yang Yunqing,Zhang Xibao,Luo Quan
Psoriasis represents a chronic inflammatory skin disease occurring globally. We investigated the role of plasma exosomal microRNA (miR)- 148a- 3p and its target gene Bim in psoriasis. Plasma exosomes (Exos) and CD4 + T cells were extracted from psoriatic patients. miR- 148a- 3p expression in Exos and CD4 + T cells of psoriatic patients, the proportions of CD4 + T cell subsets, and the contents of anti-inflammatory/pro-inflammatory factors were determined by RT-qPCR, flow cytometry and ELISA. The correlation between plasma exosomal miR- 148a- 3p and CD4 + T cell subsets in psoriatic patients was analyzed by Pearson’s analysis. The psoriatic mouse was treated with Exos/antagomir miR- 148a- 3p. Histopathological changes in the skin were observed. The CD4 + T cell subset levels, serum cytokine contents and miR- 148a- 3p expression in the blood were measured. The miR- 148a- 3p-Bim targeted binding relationship was predicted and verified by Starbase database and dual-luciferase assay. The Bim expression in psoriatic mice was determined. Psoriatic patients had highly-expressed miR- 148a- 3p in both Exos and CD4 + T cells, and abnormal CD4 + T cell subsets and cytokine levels. Plasma exosomal miR- 148a- 3p was correlated with the CD4 + T cell subsets in psoriatic patients. Exos caused down-regulated miR- 148a- 3p level in skin tissues of mouse, regulated CD4 + T cell function and aggravated the symptoms in psoriatic mice. miR- 148a- 3p repression partially reversed the role of Exos in CD4 + T cell function and psoriasis-like symptoms. Exos-carried miR- 148a- 3p targeted Bim in CD4 + T cells of psoriatic mice. Plasma exosomal miR- 148a- 3p targeted Bim to affect the dysfunction of CD4 + T cells in psoriatic mice, thereby aggravating the psoriasis-like symptoms.
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PLANT MOLECULAR BIOLOGY
·
2025
·Vol. 115
·期 2
·1-13
·
IF 3.8
Liu Yitong,Liu Shuangrong,Jing Yanjun,Li Jialong,Lin Rongcheng
Seed dormancy enables plants to delay germination until conditions are favorable for the survival of the next generation. Seed dormancy and germination are controlled by a combination of external and internal signals, in which light and ethylene act as critical regulators. However, how light and ethylene are interlinked to control these two processes remains to be investigated. Here, we show that ethylene and its precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), promote seed germination under light. Light facilitates the conversion of ACC to ethylene, in which phytochrome B (phyB) and FAR-RED ELONGATED HYPOCOTYL3 (FHY3) are functionally required. ACC oxidases (ACOs) catalyze the conversion of ACC to ethylene, among which ACO1 is specifically and predominantly expressed in imbibed seeds. Ethylene induces FHY3 protein accumulation in imbibed seeds, whereby FHY3 directly binds to ACO1 promoter and specifically mediates light-promoted ACO1 expression. Light promotes ACO1 protein accumulation. Overexpression of ACO1 significantly promotes seed germination, and almost completely restores the dormant defect of fhy3 loss-of-function mutants. In summary, this study reveals an ethylene-responsive regulatory cascade of phyB-FHY3-ACO1 that integrates external light input with internal factors to regulate seed dormancy and germination.
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Microsystems & Nanoengineering
·
2025
·Vol. 11
·期 1
·1-13
·
IF 9.9
Zheng Lixiang,Li Gong,Du Henan,Li Zonghao,Xu Bingrui,Yang Fan,Mao Yanan,Wei Jing,Xie Hainan,Xie Wei,Fu Rongxin,Liu Na,Zhang Shuailong,Liu Lianqing,Li Wen Jung,Sun Yu
Automated parallel manipulation of multiple micro-objects with optoelectronic tweezers (OET) has brought significant research interests recently. However, the parallel manipulation of multiple objects in complex obstacle-dense microenvironment using OET technology based on negative dielectrophoresis (nDEP) remain a big technical challenge. In this work, we proposed an adaptive light pattern design strategy to achieve automated parallel OET manipulation of multiple micro-objects and navigate them through obstacles to target positions with high precision and no collision. We first developed a multi-micro-object parallel manipulation OET system, capable of simultaneous image processing and microparticles path planning. To overcome microparticle collisions caused by overlapping light patterns, we employed a novel adaptive light pattern design that can dynamically adjust the layout of overlapping light patterns according to surrounding environment, ensuring enough space for each microparticle and preventing unintended escapes from the OET trap. The efficacy of this approach has been verified through systematic simulations and experiments. Utilizing this strategy, multiple polystyrene microparticles were autonomously navigated through obstacles and microchannels to their intended destinations, demonstrating the strategy’s effectiveness and potential for automated parallel micromanipulation of multiple microparticles in complex and confined microenvironments.
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BMC CANCER
·
2025
·Vol. 25
·期 1
·1-10
·
IF 3.4
Liu Shiyuan,Guan Tian,Yang Futian,Zhang Honglei,Yao Yao,Huang Jiman,Zhao Pengfei,Huang Wei,Wu Tailiang,Lin Hui,Fu Ting,Chen Shaoyu,Dai Pengzhan,Ding Zhechun,Chen Dongmei,Li Wei,Zeng Haoyu,Zhang Xianjun
Human oral tongue squamous cell carcinoma (OTSCC) is a prevalent form of head and neck squamous cell carcinoma (HNSCC), often presenting at an advanced stage with a grim prognosis. Traditional therapeutic approaches such as surgery, adjuvant radiotherapy, and chemoradiotherapy have shown limited efficacy in treating advanced OTSCC, underscoring the urgent need for innovative treatment strategies. Our bioinformatics analysis identified CD276 as a significant biomarker in OTSCC, with high protein expression levels correlating to a markedly reduced survival rate in late-stage patients. This discovery has led us to develop chimeric antigen receptor-natural killer (CAR-NK) cells derived from umbilical cord blood cells (UCBCs), specifically targeting CD276. Our aim is to investigate this novel therapeutic approach for its potential to combat OTSCC under pre-clinical conditions. Our in vitro and ex vivo studies have demonstrated that CD276-targeted CAR-NK cells exhibit remarkable efficacy in lysing OTSCC cell lines and primary cells, as well as in eliminating OTSCC organoids. These promising results underscore the pivotal role of CD276 in OTSCC pathogenesis and highlight the potential of CAR-NK cell therapy as a groundbreaking treatment option for advanced-stage OTSCC, offering new hope for translational medicine in the field of stomatology.
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Discover Oncology
·
2025
·Vol. 16
·期 1
·1-13
·
IF 2.9
Huang Hai-yi,Lin Lisa Zong Yong,Lu Xin-yi,Jiang Ying
Triple negative breast cancer (TNBC) refers to a molecular subtype of breast cancers (BC) with high rate of distant metastases and poor prognosis. Epirubicin (EPI) is widely used for the therapy of TNBC but it’s limited in clinical use due to cardiotoxicity and chemotherapy resistance. We previously identified F-α-DDB-derivative, a novel synthetic of bifendate, as a potential agent to improve the therapeutic efficacy of TNBC in combination with EPI since F-α-DDB-derivative inhibited MDA-MB-468, but not as much as EPI. In this study, we investigated the antitumor activity of F-α-DDB-derivative in combination with EPI against TNBC in vitro and in vivo and whether co-treatment would induce additional cardiotoxicity. In human TNBC MDA-MB-468 cells, application of F-α-DDB-derivative (11.5, 23.0, 46.0 μg/ml) in combination with EPI (1.5, 3.0, 6.0 μg/ml) exhibited great inhibition on cell viability and cell proliferation. Additionally, F-α-DDB-derivative (5.75, 11.5, 23.0, 46.0 μg/ml) interacted with EPI (0.75, 1.5, 3.0, 6.0 μg/ml) synergistically to induce apoptosis in MDA-MB-468 cells. This suggests that F-α-DDB-derivative may be more sensitive to apoptotic pathways. Furthermore, we revealed that co-administration of EPI and F-α-DDB-derivative (ip, once every other day for 14 days) significantly increased the therapeutic efficacy of EPI (2.0 mg/kg) or F-α-DDB-derivative (20.0 mg/kg) in mice harboring MDA-MB-468 cell xenografts without additional cardiotoxicity compared to that in EPI monotherapy group. These results implicate that co-treatment of EPI and F-α-DDB-derivative may be a potential therapeutic approach for the treatment of TNBC.
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RARE METALS
·
2025
·
IF 11
Ma Jingwen,Li Junbin,Tian Ying,Liu Jinhao,Wang Ruiting
Fe–N–C single-atom catalysts are considered among the most promising non-precious metal-based catalysts for oxygen reduction reaction (ORR), but issues such as low utilization of active sites and the easy aggregation of single atoms severely hinder their application in fuel cells and metal–air batteries. Herein, a single-atom Fe-embedded hierarchical porous hollow carbon fiber catalyst (Fe SAC/HCNF) is reported for ORR. The hollow channels and the porous structure of the fibers facilitate the exposure of single-atom active sites, and offer multidimensional mass transfer pathways to promote the transport of reactants, thus significantly enhancing catalytic performance. Additionally, the abundant micropores exert spatial confinement, which is beneficial for preventing the aggregation of single atoms. Leveraging its unique structural advantages, the Fe SAC/HCNF catalyst demonstrates outstanding ORR activity with low metal loading, boasting a high half-wave potential of 0.905 V, a substantial double-layer capacitance ( C dl ) of 41.1 mF cm −2 , and a notable kinetic current density of 45.2 mA cm −2 in alkaline media. Furthermore, the liquid Zn–air battery (ZAB) using Fe SAC/HCNF catalyst as the air cathode exhibits excellent battery performance and long-term cycling durability nearly 600 h. And the flexible quasi-solid-state ZAB can be stably cycled in various flat/bent states, which is promising for applications in flexible electronic devices. Graphical
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JOURNAL OF MOLECULAR MEDICINE-JMM
·
2024
·
IF 4.8
Wang Pengtao,Tao Wei,Li Qiujie,Ma Wanting,Jia Wei,Kang Yuting
Indole-3-aldehyde (I3A) is an intestinal microbial metabolite that regulates inflammation in various inflammatory diseases; however, its role in chronic obstructive pulmonary disease (COPD) remains unclear. This study aimed to investigate the anti-inflammatory effects and molecular mechanisms of I3A in COPD. We constructed in vivo models using cigarette smoke (CS)-stimulated mice and in vitro models using cigarette smoke extract (CSE)-stimulated MH-S cells. The results demonstrated that I3A significantly alleviated bronchial obstruction in mice with COPD and reduced the expression of inflammatory factors such as TNF-α, IL-1β, and IL-6. Additionally, I3A decreased the levels of matrix metalloproteinases MMP2, MMP12, and inhibited the NF-κB p65/NLRP3 pathways. Further investigation revealed that I3A inhibited NF-κB activity by suppressing p65 phosphorylation and nuclear translocation in CSE-stimulated MH-S cells. The activation of the NF-κB and NLRP3 signaling pathways is mediated by histone deacetylase 5 (HDAC5) and HDAC6, both of which are inhibited by I3A. Subsequent experiments indicated that aryl hydrocarbon receptor (AHR) knockdown attenuated the inhibitory effect of I3A on pro-inflammatory cytokines and the HDACs/NF-κB/NLRP3 signaling pathways, highlighting the dependence of I3A's anti-inflammatory effects on the AHR receptor. Key messages
I3A effectively reduced lung inflammation in COPD mice by inhibiting the NF-κB pathway.
In CSE-stimulated MH-S cells, I3A suppressed p65 phosphorylation and nuclear translocation, thereby inhibiting NF-κB activity.
The activation of the NF-κB/NLRP3 pathways by HDAC5 and HDAC6 was diminished by I3A.
Through the activation of the AHR receptor, I3A suppressed the activities of HDAC5/6, leading to a decrease in inflammatory factor levels.
Graphical abstract
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AMB Express
·
2021
·Vol. 11
·期 1
·1-11
·
IF 3.298
Zhao Yirui,Sang Xue,Hao Hongshun,Bi Jingran,Zhang Gongliang,Hou Hongman
Controlling the content of biogenic amines (BAs) is critical to guarantee the safety of fermented aquatic products. The degradation characteristics and application potential of amine-negative starter cultures ( Virgibacillus halodenitrificans CGMCC 1.18601: G25 , Virgibacillus pantothenticus CGMCC 1.18602: G38) screened from grasshopper sub shrimp paste (Gssp) were studied. The enzymes of the two strains G25 and G38 that degrade BAs were amine oxidases (AOs) located on their respective cell membranes. The conditions that promoted the AO activity of Virgibacillus spp. were NaCl concentrations 5–10%, temperature 37 °C, pH 7.0 and ethanol concentrations 0–2%. Safety assessments (antibiotic susceptibility, biofilm activity and hemolytic activity) indicated that Virgibacillus spp. do not present a risk to human health, and this isolate can be confidently recommended as safe starter cultures for the food industry. Then, the two strains were cultured separately as starters and applied to the Gssp to analyze their influence on the flavor and quality of the product. As far as the bad flavors in Gssp such as sulfur-organic and sulf-chlor were concerned, the response values in the starter groups by G25 and G38 were significantly reduced by 39% and 65%, respectively. For the ability of strains to degrade BAs in Gssp, G25 degraded 11.1% of histamine, 11.3% of tyramine, 15.5% of putrescine and 4.1% of cadaverine; G38 significantly degraded 10.1% of histamine, 21.8% of tyramine, 18.1% of putrescine and 5.0% of cadaverine. These results indicated that the selected species could be used as starter cultures for the control of BA accumulation and degradation in Gssp.
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JOURNAL OF MATERIALS RESEARCH
·
2024
·Vol. 39
·期 14
·2082-2094
·
IF 2.7
Gao Chenyu,Deng Jikun,He Jiaxing,Wang Zechuan,Sheng Yuanyuan,Liu Junwu,Zhong Honghai,Tong Guoqing,Jiang Yang
A novel Cu/Ti 3 C 2 T X laminated composite foil was prepared using a combination of electroplating and electrophoretic deposition. The results indicate that the average thermal expansion coefficient of the Cu/ Ti 3 C 2 T X composite foil decreased by 11.26% compared to that of pure copper. Due to the high electron mobility of Ti 3 C 2 T X and the favorable Cu/ Ti 3 C 2 T X interface, the electrical conductivity of the Cu/ Ti 3 C 2 T X composite foil exceeded that of pure copper by 8.7%, reaching 6.2147 × 10 7 S·m −1 , and its thermal conductivity increased from 381.5 W/m·K to 423.5 W/m·K. The study revealed that a thinner, looser Ti 3 C 2 T X layer with fewer layers is more favorable for copper penetration and filling, enabling a continuous network-like structure and resulting in significantly improved thermal and electrical properties of the composite copper foil. These findings position the Cu/Ti 3 C 2 T X foil as a promising candidate for electronic encapsulation and super-conductivity. Graphical Abstract
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JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Xue Weijian,Ling Xiangyu,Li Huiqian,Liu Yuhang,Zhao Bing,Yin Yanbing
In this work, a series of dibenzimidazole derivatives 1 – 4 , act as highly reversible colorimetric and fluorescent pH chemosensor, were designed and synthesized. Excellent reversible pH response of these sensors could be found by a specific pH change through obvious fluorescent color changes. The response is not affected by common cations (including Al 3+ , Cu 2+ , Ca 2+ , Cd 2+ , Co 2+ , Cr 3+ , Mg 2+ , Na + , K + , Ni 2+ , Pb 2+ and Zn 2+ ) and anions (including F − , Cl − , Br − , I − , ClO 4 − , H 2 PO 4 − , HSO 4 − , HCO 3 − and CH 3 COO − ). Notably, these sensors can be reused more than 10 times without losing functionality. Unlike previous reports, the distinct properties of 1 – 4 are attributed to the varied link groups. Based on comprehensive experimental data and mechanistic analyses, it is concluded that sensors 1 – 4 are promising candidates for use as highly reversible “on-off-on” fluorescence switches under precise pH control. Graphic Abstract
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CATALYSIS LETTERS
·
2024
·
IF 2.3
Hu Jingru,Jiang Minjun,Hu Bingwen,Cheng Gang
Remediation of antibiotics by photocatalysis technique has been regarded as a promising route to tackle with the environmental pollution affecting human survival and development. In this work, brookite TiO 2 nanorods with different oxygen vacancy have been synthesized through tailoring the volume of ethylene glycol by hydrothermally treatment with NaTiOx-nanoassembly. After constitute and morphology confirmation with different characterizations, their photocatalytic performances are evaluated via ciprofloxacin (CIP) antibiotic degradation experiments. The result shows that the TiO 2 -0 has the highest photocatalytic efficiency towards CIP degradation, comparing with TiO 2 -15 and TiO 2 -30 samples. Although the TiO 2 -30 has high concentration oxygen vacancy, it exhibits excellent adsorption ability in the dark, rather than CIP degradation rate. The photo/electrochemical tests suggest the photo-generated electron lifetime, charge transfer ability, and the effective active sites on the material’s surface are inversely proportional to the concentration of oxygen vacancies. It also concludes that rational fabrication conditions tailoring of the photocatalyst could optimize the corresponding capability in the antibiotic degradation process. In addition, the possible degradation pathway is also proposed based on the high resolution mass spectrometry (HRMS), and the acute toxicity changes in the degradation process are also predicted. Graphical Abstract
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危险品化学品经营许可证（带存储）