CARBOHYDRATE POLYMERS
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2023
·
IF 11.2
Ronghua Tan,Jing Ge,Congcong Wang,Ying Wan,Xiangliang Yang
A novel type of diselenide bond-bridged hydroxyethyl starch-doxorubicin conjugate, HES-SeSe-DOX, was synthesized via a specially designed multistep synthetic route. The optimally achieved HES-SeSe-DOX was further combined with photosensitizer, chlorin E6 (Ce6), to self-assemble into HES-SeSe-DOX/Ce6 nanoparticles (NPs) for potentiating chemo-photodynamic anti-tumor therapy via diselenide-triggered cascade actions. HES-SeSe-DOX/Ce6 NPs were observed to disintegrate through the cleavage or oxidation of diselenide-bridged linkages in response to the stimuli arising from glutathione (GSH), hydrogen peroxide and Ce6-induced singlet oxygen, respectively, as evidenced by the enlarged size with irregular shapes and cascade drug release. In vitro cell studies exhibited that HES-SeSe-DOX/Ce6 NPs in combination with laser irradiation effectively consumed intracellular GSH and promoted a large rise in levels of reactive oxygen species in tumor cells, actuating the disruption of intracellular redox balance and the enhanced chemo-photodynamic cytotoxicity against tumor cells. The in vivo investigations revealed that HES-SeSe-DOX/Ce6 NPs were inclined to accumulate in tumors with persistent fluorescence emission, inhibited tumor growth with high efficacy and had good safety. These findings demonstrate the potential of HES-SeSe-DOX/Ce6 NPs for use in chemo-photodynamic tumor therapy and suggest their viability for clinical translation.
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JOURNAL OF MOLECULAR LIQUIDS
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2023
·
IF 6
Dan Yang,Yang Luo,Shang Wei Yuan,Li Xia Chen,Pei Hua Ma,Zhu Tao,Xin Xiao
In recent years, most of the constructions on cucurbit[ n ]uril-based supramolecular polymers have been based on host–guest chemistry, however, this limits the choice of host and guest. Herein, we present a newly designed supramolecular polymer, namely Q[8]@PEG-HPTS formed by utilizing outer surface Q[8] interactions. In aqueous solution, the supramolecular polymer Q[8]@PEG-HPTS was obtained quickly and simply by mixing Q[8] with PEG-HPTS, where after, a variety of characterization techniques were employed to confirm the structure of the Q[8]@PEG-HPTS. The ability of this system to selectively recognize metal ions as well as pesticide molecules was also investigated; selectivity was observed for Fe 3+ and paraquat. The biocompatibility and excellent fluorescence properties associated with the constituents of the supramolecular polymer allowed for the imaging of HeLa cells. This system could image and trace the nucleus through the cytoplasm, indicating that it could enter and internalize HeLa cells. Finally, by continuously adjusting the ratio between Q[8] and PEG-HPTS, a Q[8]@PEG-HPTS hydrogel was obtained. This hydrogel exhibited a stimulated temperature response, and opens up further possibilities for exploring the applications of such supramolecular polymers.
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CHEMOSPHERE
·
2023
·
IF 8.8
Dayang Zheng,Jiali Zou,Hao Xu,Min Wu,Yayi Wang,Cang Feng,Eryang Zheng,Teng Wang,Yuxiang Shi,Yongjian Chen,Binyang Li
A novel and recyclable composite material, Fe 3 O 4 /N co-doped sludge biochar (FNBC), was developed from original sludge biochar (BC) and found to have excellent stability and superior catalytic capacity during the ciprofloxacin (CIP) degradation under the action of peroxymonosulfate (PMS). In the FNBC/PMS system, an approximately complete removal of CIP was achieved within 60 min under the condition of 1.0 g/L FNBC, 3.0 mM PMS, and 20 mg/L CIP, which was about 2.08 times of that in BC/PMS system (48.01%). Besides, FNBC/PMS system could effectively remove CIP under the influence of wide pH (2.0–10.0) or inorganic ions compared with BC/PMS system. Moreover, it was found that there were radical produced under the effect of Fe element, defects, functional groups, pyridinic N and pyrrolic N and non-radical caused by graphitic N, carbon atoms next to the iron atoms and better adsorption capacity in the FNBC/PMS system. It was observed that the contribution of hydroxyl radical (•OH), sulfate radical (SO 4 •− ) and singlet oxygen ( 1 O 2 ), which were the main reactive oxygen species , during the CIP degradation, were 75.80%, 11.49% and 10.26%, respectively. Furthermore, total organic carbon (TOC) variation was analyzed and the degradation pathway of CIP was speculated. The application of this material could combine the recycling of sludge with the effective degradation of refractory organic pollutant, providing an environmentally friendly and economic method.
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Experimental and Therapeutic Medicine
·
2023
·Vol. 25
·期 4
·1-11
·
IF 2.7
Chun-Lian Liang,Xiu-Li Li,Xiao-Juan Quan,Lin Zhang
Idiopathic pulmonary fibrosis (IPF) is a heterogeneous lung disease associated with high mortality. Disabled‑2 (DAB2), an adapter protein, regulates cell‑fibrinogen adhesion and fibrinogen uptake. DAB2 is differentially expressed in mouse fibrotic lungs induced by bleomycin according to a genome microarray analysis based on Gene Expression Omnibus database. However, the role of DAB2 in IPF has not been revealed. A bleomycin‑induced mouse model of pulmonary fibrosis was constructed in the present study. It found that the expression of DAB2 was upregulated in bleomycin‑induced fibrotic lung tissue with collagen fiber deposition and pulmonary interstitium thickening. Colocalization of DAB2 with α‑smooth muscle actin (SMA) was observed in lung tissue sections. In vitro, human lung fibroblast MRC‑5 cells were treated with TGF‑β1 and the expression of DAB2 was increased. Knockdown of DAB2 suppressed cell proliferation and the expression of α‑SMA, collagen I, collagen IV and fibronectin in TGF‑β1‑treated MRC‑5 cells. The phosphorylation levels of PI3K and AKT were suppressed in DAB2‑knockdown cells. IGF‑1/IGF‑1R has been reported to promote pulmonary fibrosis and activate the PI3K/Akt signaling. In the present study, the activation of IGF‑1/IGF‑1R signaling pathways in bleomycin‑induced fibrotic lung tissues were positively associated with DAB2 expression. The phosphorylation level of IGF‑1R was increased in MRC‑5 cells with TGF‑β1 treatment, and DAB2 expression was decreased by silencing of IGF‑1R. This suggested that DAB2 might be a downstream target of the IGF‑1R pathway and thus induced PI3K/AKT signaling activation and fibrogenesis. The current study demonstrated the importance of DAB2 in pulmonary fibrosis and suggested the potential of IGF‑1R/DAB2/PI3K in the pathogenesis of IPF.
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International Journal of Nanoscience
·
2023
·
IF 0.8
Yang Fu,Chaohui Ji,Zhiheng Ma,Defeng Xu,Hang Hu
In this work, we synthesized lactobionic acid-decorated diselenide-linked polyethylene glycol-doxorubicin conjugate (LA-PEG-SeSe-DOX) and prepared free DOX-loaded LA-PEG-SeSe-DOX(DOX@LA-PEG-SeSe-DOX) nanoparticles for hepatoma-targeted DOX delivery. LA-PEG-SeSe-DOX can self-assemble into nanoparticles in deionized water and DOX@LA-PEG-SeSe-DOX nanoparticles were prepared by loading free DOX into LA-PEG-Se-Se-DOX nanoparticles under sonication. DOX@LA-PEG-SeSe-DOX nanoparticles have high DOX loading content of 31.3%. The dynamic scattering analysis shows that DOX@LA-PEG-SeSe-DOX nanoparticles have small size (hydrodynamic diameter 4 5 . 6 9 ± 0 . 7 1 nm), near neutral zeta potential, and excellent colloidal stability. The in vitro drug release study indicates that DOX@LA-PEG-SeSe-DOX nanoparticles exhibit dual redox-responsive drug release characteristics. The cellular uptake study reveals that DOX@LA-PEG-SeSe-DOX nanoparticles can be taken up by hepatoma cells by asialoglycoprotein receptor (ASGPR)-mediated pathway. Finally, DOX@LA-PEG-SeSe-DOX nanoparticles exhibit enhanced cytotoxicity against HepG2 cells as compared to LA-PEG-SeSe-DOX nanoparticles, underlining the significance of releasing free DOX for effective tumor cell proliferation inhibition. This work provides a facile and effective strategy for targeted delivery of DOX to hepatoma cells.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Ying Peng,Yongshuai Xie,Zhezhe Deng,Dehua Ma,Benxue Liu,Xinqiang Wang,Guanghui Zhang,Luyi Zhu
The inherent brittleness and hydrophilicity of ceramics pose a great challenge to designing a reliable structure that can resist mechanical loads and moisture in extreme conditions with high temperature and high humidity. Here, we report a two-phase hydrophobic silica–zirconia composite ceramic nanofiber membrane (H-ZSNFM) with exceptional mechanical robustness and high-temperature hydrophobic resistance. For the dual-phasic nanofibers, the amorphous silica blocked the connection of zirconia nanocrystals, and the lattice distortion was observed due to Si in the ZrO2 lattice. H-ZSNFM has strong strength (5–8.4 MPa), high hydrophobic temperature resistance (450 °C), high porosity (89%), low density (40 mg/cm3), low thermal conductivity (30 mW/m·K), and excellent thermal radiation reflectivity (90%). By simulating the actual high-temperature and high-humidity environment, 10-mm-thick H-ZSNFMs can reduce the heat source from 1365 to 380 °C and maintain complete hydrophobicity even in a water vapor environment of 350 °C. This means that it has superior insulation and waterproof performance even in a high-temperature water environment. For firefighting clothing, H-ZSNFM displayed waterproof and insulation layers, which have excellent thermal protection performance and achieve incompatibility between water and fire, providing valuable time for fire rescue and a safety line of defense for emergency personnel. This design strategy with mechanical robust and hydrophobic temperature resistance applies to the development of many other types of high-performance thermal insulation materials and presents a competitive material system for thermal protection in extreme conditions.
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EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Peiyun Cheng,Na Liang,Wei Zhao,Xianfeng Gong,Weijia Wang,Shaoping Sun
In this study, an amphiphilic polymer mPEG-CS-Bio-Hz-Q with capabilities of near-infrared (NIR) fluorescence imaging, active tumor targeting, minimized protein adsorption and pH-responsive drug release was designed and synthesized. The polymer was served as a micellar carrier for paclitaxel (PTX) delivery. The mean size of PTX-loaded mPEG-CS-Bio-Hz-Q micelles was 123.5 nm. In vitro drug release study indicated the pH-triggered PTX release behavior of the micelles. Cellular uptake study demonstrated that the micelles could be effectively internalized by MCF-7 cells and exhibit bright fluorescence. In vivo investigation illustrated the high antitumor activity and excellent safety of the micelles. The mPEG-CS-Bio-Hz-Q micellar system would be promising candidate for bioimaging and cancer therapy.
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Drug Design Development and Therapy
·
2023
·
IF 4.8
Junhui He,Dongbo Han,Chunlian Jia,Jiaxiu Xie,Fucui Zhu,Jie Wei,Dongmei Li,Dongmei Wei,Yi Li,Li Tang,Guining Wei,Jing Yan,Yuanming Tong,Lifang Yang,Xuecai Tan
Purpose To investigate the mechanisms of antidepressant action of active fraction of Polyrhachis vicina Rogers (AFPR) through network pharmacology, molecular docking and experimental validation.Methods GC-MS was used to predict chemical compounds, corresponding databases were used to predict chemical compound targets and depression targets, Cytoscape software was used to construct and analyze the protein interaction network map, DAVID database was used to analyze gene ontology (GO) and KEGG signaling pathway, and AGFR software was used to perform molecular docking. Subsequently, the underlying action mechanisms of AFPR on depression predicted by network pharmacology analyses were experimentally validated in a CORT-induced depression model in vitro and in vivo.Results A total of 52 potential targets of AFPR on antidepressant were obtained. GO is mainly related to chemical synaptic transmission, signal transduction and others. KEGG signaling pathways are mainly related to cAMP signaling pathway and C-type lectin receptor signaling pathway. The experiment results showed that AFPR significantly increased the expression of PRKACA, CREB and BDNF in mouse brain tissue and PC12 cells. Furthermore, after interfered of cAMP in PC12 cells, the decreased expression of PRKACA, CREB and BDNF was reversed by AFPR.Conclusion AFPR may exert antidepressant effects through multiple components, targets and pathways. Furthermore, it could improve neuroplasticity via the cAMP signaling pathway to improve depression-like symptoms.
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ENVIRONMENTAL TECHNOLOGY
·
2023
·
IF 2.8
Wenyan Wang,Yunlan Xu,Dengjie Zhong
Ni-EDTA is widely present in electroplating effluents. It cannot be effectively removed by traditional wastewater treatment methods due to its chemical stability. In this study, copper sulphide/cuprous sulphide doped zero-valent iron@carbon (ZVI@C/CuS/Cu2S) was prepared to active peroxymonsulphate (PMS) to decomposition Ni-EDTA. The ZVI@C/CuS/Cu2S + PMS process shows excellent performance under neutral or even alkaline conditions. This is due to the acceleration of ZVI electron transport by CuS/Cu2S, the autocatalysis of CuS/Cu2S itself, and the synergistic effect of CuS/Cu2S and Ni-EDTA. The removal efficiency of 50 ppm Ni-EDTA electroplating effluents reached 99.53% at 10 min, and the discharge water can meet the Chinese emission standard. The influences of the main parameters such as initial pH value, catalyst, PMS and initial Ni-EDTA concentration on removal efficiency was systematically investigated.
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DRUG DEVELOPMENT RESEARCH
·
2023
·Vol. 84
·期 3
·582-600
·
IF 3.8
Jiabin Zhou,Yuhan Liu,Jun Chen,Nanxiang Xiong,Dongye Yi
Kaempferol (Kae) is a natural flavonoid that has multiple biological activities, such as anti-inflammatory and antitumor activities. However, few studies have been reported on antiglioma effects of Kae. This study aimed to explore the effects and potential mechanisms of Kae and synergistic antitumor activities with gefitinib (Gef) on glioma. Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine assays were used to detect cytotoxicity and cell proliferation. Cell apoptosis and the cell cycle were detected by flow cytometry. Transwell assays were used to detect the migratory and invasive abilities of glioma cells. Network pharmacology and molecular docking analysis were used to screen for core targets of Kae in glioma therapy. Xenograft tumor nude mice were established with U251 cells to verify the antiglioma effects of Kae in vivo. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay was used to detect apoptosis in tumor tissues. The expression of proteins was detected by immunohistochemistry and western blot analysis. Kae inhibited cell proliferation, promoted apoptosis, and induced cell cycle arrest in the G2/M phase of glioma cells in a concentration-dependent manner. Kae inhibited the migration and invasion of glioma cells at low concentrations. Network pharmacology analyses showed that epidermal growth factor receptor (EGFR) and SRC proto-oncogene (SRC) might be direct molecular-binding targets of Kae. Our results showed that Kae inhibited the levels of phosphorylated EGFR, phosphorylated SRC (p-SRC), and phosphorylated signal transducer and activator of transcription 3 (STAT3). In addition, the combination of Kae with Gef significantly inhibited the proliferation of glioma cells. Kae further inhibited EGFR phosphorylation after treatment with Gef. Similarly, Kae further enhanced the inhibition of p-SRC caused by SU6656. Finally, we demonstrated that Kae exerted great antitumor activities and enhanced the antitumor effect of Gef by inhibiting EGFR/SRC/STAT3 signaling pathway in vivo. Kae played a potential role and synergistic antiglioma effects with Gef by inhibiting the phosphorylation of EGFR/SRC dual targets. Kae is expected to be a candidate drug or chemosensitizer in glioma therapy.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 6
·3458-3468
·
IF 3.3
Mingyan Liu,Xingzi Yao,Gaowei Zhang,Shengxiang Yi,Xueyong Huang,Fangjian Ning,Liping Luo
Summary Eurya honey (EH) is produced from the nectar of Eurya. In this paper, the physicochemical properties (moisture, free acidity, pH, 5-hydroxymethylfurfural, conductivity, sugar, and colour) of EH were systematically studied for the first time. The total phenols and flavonoids were determined by spectrophotometry, and the contents were 40.29 ± 4.17 mg GAE/100 g and 12.44 ± 0.62 mg RE/100 g, respectively. A total of 232 compounds were identified, and 30 of them were quantified by ultra-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q/TOF-MS). The potential markers of EH were abscisic acid (21.3–103.4 μg/100 g), gallic acid (6.9–220.2 μg/100 g), tricoumaroyl spermidine (284.2–518.1 μg/100 g), and kaempferol (15.0–258.7 μg/100 g). EH showed antioxidant capacities in DPPH free radical-scavenging activity (IC 50 : 42.87–131.26 mg mL −1 ), ferric reducing antioxidant capacity (23.22–112.24 μmol FeSO 4 ·7H 2 O/100 g), and ABTS radical-scavenging activity assays (9.51–31.21 μmol Trolox/100 g).
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Foods
·
2023
·Vol. 12
·期 5
·1031
·
IF 5.2
Jing-Hong Liu,Yu-Yun Zheng,Jian-Ming Ouyang
The antioxidant activities of seven degraded products (GLPs) with different molecular weights (Mw) of polysaccharides fromGracilaria lemaneiformiswere compared. TheMwof GLP1–GLP7 were 106, 49.6, 10.5, 6.14, 5.06, 3.71 and 2.42 kDa, respectively. The results show that GLP2 withMw= 49.6 kDa had the strongest scavenging capacity for hydroxyl radical, DPPH radical, ABTS radical and reducing power. WhenMw< 49.6 kDa, the antioxidant activity of GLPs increased with the increase inMw, but whenMwincreased to 106 kDa, their antioxidant activity decreased. However, the ability of GLPs to chelate Fe2+ions increased with the decrease in polysaccharideMw, which was attributed to the fact that the polysaccharide active groups (–OSO3–and –COOH) were easier to expose, and the steric hindrance was smaller when GLPs chelated with Fe2+. The effects of GLP1, GLP3, GLP5 and GLP7 on the crystal growth of calcium oxalate (CaOx) were studied using XRD, FT-IR, Zeta potential and thermogravimetric analysis. Four kinds of GLPs could inhibit the growth of calcium oxalate monohydrate (COM) and induce the formation of calcium oxalate dihydrate (COD) in varying degrees. With the decrease inMwof GLPs, the percentage of COD increased. GLPs increased the absolute value of the Zeta potential on the crystal surface and reduced the aggregation between crystals. Cell experiments showed that the toxicity of CaOx crystal regulated by GLPs to HK-2 cells was reduced, and the cytotoxicity of CaOx crystal regulated by GLP7 with the smallestMwwas the smallest, which was consistent with the highest SOD activity, the lowest ROS and MDA levels, the lowest OPN expression level and the lowest cell necrosis rate. These results suggest that GLPs, especially GLP7, may be a potential drug for the prevention and treatment of kidney stones.Keywords:Gracilaria lemaneiformispolysaccharide;antioxidant activity;cytotoxicity;calcium oxalate;molecular weight
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