Advanced Healthcare Materials
·
2025
·
IF 9.6
Yu Hu Zhou,Zi Hao Zou,Jian Xiang Teng,Zhan Yu Wu,Si Wei Luo,Xu Ning,Chuan Ye,Long Yang,Wei Seong Toh
Avascular necrosis of the femoral head (ANFH) is a debilitating musculoskeletal disorder that is typically caused by impaired blood supply to the hip joint. In treating irregular bone defects that resulted from ANFH, injectable hydrogels are particularly attractive as they can be administered in a minimally invasive manner and conform to the variable shape of a bone defect. However, they often lack the biochemical and mechanical properties for effective bone repair. To address these issues, an injectable composite hydrogel, SA/β-TCP@PMP, composed of sodium alginate (SA) and β-tricalcium phosphate (β-TCP) crosslinked with glucono-delta-lactone (GDL), and enforced with varying concentrations of poly(3-hydroxybutyrate- co -4-hydroxybutyrate)/magnesium oxide and polyethylene glycol (P34HB/MgO+PEG; PMP) coaxial electrospun microfibers are formulated. By varying the concentrations (0%, 1%, 2%, 4%) of PMP microfibers, the physical properties of the composite hydrogel, including its injectability, surface morphology, swelling, degradation, and mechanical properties, as well as magnesium ions (Mg 2+ ) release can be finely tuned. Among all, SA/β-TCP@2%PMP composite hydrogel demonstrates superior angiogenic and osteogenic properties, and promotes robust new bone formation in a rabbit model of steroid-induced ANFH. Overall, this novel composite hydrogel offers significant application potential for minimally invasive treatment of irregular bone lesions in ANFH.
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PubMed
高影响因子
CELLULOSE
·
2025
·
IF 4.8
Zhang Yutian,Zeng Dongdong,Li Jianing,Ma Xiaojun,Li Dongna,Li Yachao
Co-substrate fermentation has become an essential approach to achieving high-efficiency and low-cost production of poly (β- l -malic acid) (PMA). Herein, sustainable rubberwood ( Hevea brasiliensis ) hydrolysate (RWH) was co-fermented by A. pullulans to synthesize PMA using glucose and xylose as co-substrate carbon sources. Moreover, vitamin B was explored as nutrient supplementation in this study. Results showed that compared with the single RWH substrate, the PMA production of co-substrate fermentation increased by 38–52%. Furthermore, the addition of VB 6 promoted the utilization of substrates and shortened the fermentation cycle of PMA. During the scale-up fermentation, the PMA yield reached 0.50–0.54 g/g. Compared with traditional glucose culture medium, co-substrate fermentation improved the conversion rate of carbon sources and reduced PMA production cost by 14.9–36.6%. This work provides a new path for high-efficiency and economical industrial fermentation technology of PMA.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Kaiyu Jiang,Wei Gong,Yu-Xuan Liang,Juncai Tu,Jinjin Li,Weiman Gao,Xiuping Dong,Beiwei Zhu
To investigate the effects of processing temperatures on α, β-unsaturated aldehyde-mediated modifications of cod protein, a clickable probe, 4-(2-Propyn-1-yloxy)-2-butenal (yne-ACR), was developed to simulate α, β-unsaturated aldehyde modifications on cod protein in simulated processing systems (25 °C, 90 °C, and 180 °C). Processing at 180 °C resulted in a 3-fold increase in the hydrophobicity, while modifications under thermal processing had limited effects on the content of free nucleophilic amino acids. However, modification at 180 °C significantly increased the content of carbonylated proteins to 14.7 nmol/mg protein. Cod protein modified during thermal processing (90 °C and 180 °C) exhibited superior emulsifying and foaming properties than those subjected to heating alone. Cod protein processed at 90 °C exhibited 757 identified modified components, representing the highest number among the three processing temperatures, and thermally induced structural changes in cod protein facilitated multiple modifications on a single peptide. These findings provide new tools and insights for the study of food protein modification.
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高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Liyun Zhang,Yi He,Lan Ma,Jingyu Chen,Yi Fan,Shihong Zhang,Heng shi,Zhenyu Li,Pingya Luo
Filtration membranes, with good antifouling performance and robust solvent resistance (e.g., organic solvents or highly acidic/alkaline/saline solvents), that can effectively purify complex polluted water systems are especially demanded in practice but present a challenge to be conquered. Herein, a simple method has been demonstrated to address the obstacles, applying the stabilized polyacrylonitrile (SPAN) nanofiber/β-FeOOH nanorod composite membrane as a model. In this work, simply stabilizing PAN nanofibers in air can achieve robust solvent resistance against organic solvents and strong inorganic acidic/alkaline/saline solutions. Hydrophilic β-FeOOH nanorods were anchored onto SPAN nanofibers of our electrospun membrane and achieve superhydrophilicity (0°)/underwater superoleophobicity (>155°) for various oils. More importantly, the SPAN/β-FeOOH nanofibrous membrane exhibits robust mechanical strength (274 MPa of Young’s modulus), excellent chemical stability, fast separation flux (2532–10146 L m–2 h–1), and satisfying removal ratio (>98.2%) against insoluble oils and soluble cationic dyes. In addition, good photocatalytic activity against organic pollutants provides our membranes with excellent flux restorability and a long-term use capacity. These outstanding performances endow our membrane with a great potential application in purifying polluted aquatic systems in worldly harsh conditions.
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PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 12
·3369
·
IF 3.098
Li-Min Zhao,Zhen Guo,Yi-Jie Xue,Jun Zhe Min,Wen-Jing Zhu,Xiang-Yu Li,Hu-Ri Piao,Cheng Hua Jin
The transforming growth factor-β (TGF-β), in which overexpression has been associated with various diseases, has become an attractive molecular target for the treatment of cancers. Thirty-two quinoxaline-derivatives of 3-substituted-4-(quinoxalin-6-yl) pyrazoles14a–d,15a–d,16a–d,17a–d,18a–d,19a–d,25a,25b,25d,26a,26b,26d,27b, and27dwere synthesized and evaluated for their activin TGF-β type I receptor kinase and p38α mitogen activated protein (MAP) kinase inhibitory activity in enzymatic assays. Among these compounds, the most active compound19binhibited TGF-β type I receptor kinase phosphorylation with an IC50value of 0.28 µM, with 98% inhibition at 10 µM. Compound19balso had good selectivity index of >35 against p38α MAP kinase, with 9.0-fold more selective than clinical candidate, compound3(LY-2157299). A molecular docking study was performed to identify the mechanism of action of the synthesized compounds and their good binding interactions were observed. ADMET prediction of good active compounds showed that these ones possess good pharmacokinetics and drug-likeness behavior.Keywords:TGF-β;ALK5 inhibitor;kinase assay;selectivity;docking
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高影响因子
Desalination and Water Treatment
·
2016
·
IF 1.272
Xudong Zheng,Jiangdong Dai,Jianming Pan
In this work, mesoporous attapulgite (mATP) was firstly prepared in the presence of the cetyltrimethylammonium bromide using attapulgite (ATP) as the matrix materials. Then, it was modified by β-cyclodextrin (β-CD) to obtain the β-cyclodextrin/mesoporous attapulgite composites (β-CD/mATP). The characterization of the composites showed that β-CD/mATP composites remained excellent channel structure and the value of the specific surface area was 300.30 m2/g. As-prepared β-CD/mATP was evaluated as sorbents to adsorption of 2,4,6-trichlorophenol (2,4,6-TCP) and 2,4,5-trichlorophenol (2,4,5-TCP). The results of the batch mode experiment revealed that the Langmuir isotherm model fitted the equilibrium data better, while the kinetic data could be described by pseudo-second-order model perfectly. Furthermore, 2,4,5-TCP adsorption onto β-CD/mATP was more affected than that of 2,4,6-TCP in the presence of competitive phenolic compound. In addition, β-CD/mATP possessed excellent regeneration property.
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高影响因子
DALTON TRANSACTIONS
·
2016
·Vol. 45
·期 9
·3853-3862
·
IF 4.177
Ao Wang,Weiwei Jin,Enyi Chen,Jiahong Zhou,Lin Zhou,Shaohua Wei
Near-infrared (NIR) light triggered photodynamic therapy (PDT), based on upconversion nanoparticles (UCNPs), has attracted great attention because of its high tissue penetration and low photodamage to living organisms. However, most UCNPs cannot be stably dispersed in aqueous solution and cannot carry photosensitive drugs directly. Besides, UCNP mediated PDT is a fluorescence resonance energy transfer (FRET) process from the UCNPs to the attached photosensitive drugs. So the drug and UCNPs must be stably connected and close enough. In this manuscript, carboxymethyl-β-cyclodextrin (COOH-β-CD) was used to connect UCNPs and adamantine modified phthalocyanine (Ad-ZnPc) through a self-assembly process followed by a host–guest interaction. COOH-β-CD can provide good water solubility of the system and short-distance linking between the UCNPs and Ad-ZnPc. Most importantly, the system has a strong NIR light triggered PDT activity toward cancer cells.
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高影响因子
Acta Biomaterialia
·
2015
·
IF 6.025
Xin-Shi Wang,Jun-Qing Situ,Xiao-Ying Ying,Hui Chen,Hua-fei Pan,Yi Jin,Yong-Zhong Du
Multifunctional nanoparticles which integrate the therapeutic agents and bio-imaging agents into one carrier are emerging as a promising therapeutic platform. Herein, GaOOH:Cr 3+ was firstly synthesized using improved hydrothermal method (atmospheric pressure, 95 °C), and by manipulating the pH of the reaction medium, GaOOH:Cr 3+ with different sizes (125.70 nm, 200.60 nm and 313.90 nm) were synthesized. Then β-Ga 2 O 3 :Cr 3+ nanoparticles with porous structures were developed as a result of the calcination of GaOOH:Cr 3+ . The fabricated, porous β-Ga 2 O 3 :Cr 3+ nanoparticles could effectively absorb doxorubicin hydrochloride (DOX) (loading rate: 8% approximately) and had near infrared photoluminescence with a 695 nm emission. Furthermore, β-Ga 2 O 3 :Cr 3+ nanoparticles were coated with l -Cys modified hyaluronic acid (HA-Cys) by exploiting the electrostatic interaction and the cross-link effect of disulfide bond to improve the stability. The DOX loaded HA-Cys coated β-Ga 2 O 3 :Cr 3+ nanoparticles (HA/β-Ga 2 O 3 :Cr 3+ /DOX) showed an oxidation–reduction sensitive drug release behavior. The HA-Cys coated β-Ga 2 O 3 :Cr 3+ nanoparticles showed a low cytotoxicity on MCF-7 and Hela cell lines. The cellular uptake of HA/β-Ga 2 O 3 :Cr 3+ /DOX using the near infrared photoluminescence of β-Ga 2 O 3 :Cr 3+ nanoparticles and the fluorescence of DOX demonstrated the HA/β-Ga 2 O 3 :Cr 3+ /DOX could internalize into tumor cells quickly, which was affected by the size and shape of β-Ga 2 O 3 :Cr 3+ nanoparticles.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jingming Wang,Bin Wu,Na Zhang,Mingyue Zhao,Tingting Jing,Yi Wu,YunQing Hu,Feng Yu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
β-Ionone is a carotenoid-derived flavor and fragrance compound with a complex fruity and woody scent, known for its violet aroma. Due to the low odor threshold, β-ionone dramatically affects the aroma and quality of tea. Previous studies have shown that β-ionone increases during tea withering; however, its formation and regulation during the withering process are far from being understood. As dehydration is the most important stress during the withering of the tea leaves, we isolated a dehydration-induced gene belonging to the subfamily of carotenoid cleavage dioxygenases called carotenoid cleavage dioxygenase 1a (CsCCD1a) from Camellia sinensis and expressed it in Escherichia coli. The recombinant protein could generate volatile β-ionone and pseudoionone from carotenoids. CsCCD1a was induced by dehydration stress, and its expression was related to the β-ionone accumulation during tea withering. Overall, this study elucidated that CsCCD1a catalyzes the formation of β-ionone in C. sinensis and enhanced the understanding of the β-ionone formation under multiple stresses during the processing of tea.
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PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 59
·51110-51119
·
IF 4.6
Puying Qi,Hongwu Liu,Yue Li,Daiyu Tang,Lei Shao,Junjie Wang,Qifei Zhu,Taibai Jiang,Lixia Li,Shilong Jiang,Fan Wu,Yanhong Guo,Yong Liu,Lihong Shi,Yin Wang,Jun Sun
Xanthomonas oryzae pv. Oryzae, result in rice bacterial blight, is the most severe bacterial disease affecting rice, and in certain regions, it is considered the most critical disease overall, with the potential to reduce yields by as much as 50%. It is difficult to control rice bacterial blight and lacking of pesticides. 1,2,3,4-Tetrahydro-β-carboline (THC) and their analogues show a diverse range of activities; however, research specifically focusing on THC remains limited, particularly concerning its antibacterial properties. Given its promising characteristics, THC holds potential for development as an environmentally friendly green pesticide. These outcomes reveal that THC signally inhibits both the cell growth and biofilm formation, thereby reducing its pathogenicity. Consequently, THC holds promise as a novel green pesticide aimed at targeting bacterial biofilms to effectively manage rice bacterial leaf blight.
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高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Lvyuan Chen,Kungang Chai,Dongli Li,Youquan Zhang,Fang Lai
Under the challenge of efficiently removing bisphenols (BPs) from contaminated water, we herein present the synthesis and evaluation of two rigid aromatic β-cyclodextrin polymers with hierarchical porous networks (RACP-HPNs) via an integrated polymerization–crosslinking strategy. The materials were characterized by multiple techniques to verify their structures. Adsorption experiments revealed that RACP-HPN exhibited an excellent overall adsorption performance and rapid kinetics. Its adsorption capacity for BPA reaches 542.2 mg g −1 , which equilibrates within only 3 min, and it retains over 96 % of the adsorption performance after six regeneration cycles. Effective removal was achieved under various solution conditions, and the performance was closely dependent on the molecular configuration and size of the bisphenol substrates. Theoretical calculations confirmed that the efficient adsorption originated primarily from van der Waals forces, hydrogen bonding and π–π interactions, while its hierarchical pore architecture promotes mass transfer and accessibility to active sites. These findings indicate that RACP-HPN is a promising and rigid adsorbent for efficient removal of bisphenol compounds.
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高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Tao Bo,Chenfei Wang,Dingjin Yao,Qiuyu Jiang,Yitong Zhao,Feifei Wang,Wei He,Weiyi Xu,Hao Zhou,Ming Li,Si Zhang,Ruyi Xue
Gene delivery to macrophages holds great promise for cancer immunotherapy. However, traditional gene delivery methods exhibit low transfection efficiency in macrophages. The star -shaped topological structure of polymers is known to encapsulate genes inside their cores, thereby facilitating sustained release of the genetic material. Herein, combining the structural advantages of star polymers and the transfection advantages of poly (β-amino ester)s (PAEs), we developed a novel linear oligomer grafting-onto strategy to synthesize a library of multi-terminal star structured PAEs (SPAEs), and evaluated their gene delivery efficiency in various tissue cells. The transfection with human hepatocellular carcinoma cells (HepG2, HCC-LM3 cells and MHCC-97H cells), rat normal liver cells (BRL-3 A cells), human ovarian cancer cells (A2780 cells), African green monkey kidney cells (Vero cells), human cervical cancer cells (HeLa cells), human chondrosarcoma cells (SW1353 cells), and difficult-to-transfect human epidermal keratinocytes (HaCaT cells) and normal human fibroblast cells (NHF cells) showed that SPAEs exhibited superior transfection profile. The GFP transfection efficiency of top-performing SPAEs in HeLa cells (96.1%) was 2.1-fold, and 3.2-fold higher compared to jetPEI and Lipo3000, respectively, indicating that the star-shaped topological structure can significantly enhance the transfection efficiency of PAEs. More importantly, the top-performing SPAEs could efficiently deliver Nod2 DNA to difficult-to-transfect RAW264.7 macrophages, with a high transfection efficiency of 33.9%, which could promote macrophage M1 polarization and enhanced CD8+ T cell response in co-incubation experiments. This work advances gene therapy by targeting difficult-to-transfect macrophages and remodeling the tumor immune microenvironment.
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