ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Zhang Hongding,Ke Changlu,Xing Zhenhua,Zhao Mengqi,Xiang Xin,Wang Hai-Bo
Although fluorescent probes have been used extensively for alkaline phosphatase (ALP) sensing, most involve a tedious, prolonged, or high-cost synthesis procedure. Thus, it is still necessary to develop convenient and economical fluorescent probes for sensitive determination of ALP. Herein, a facile fluorescent strategy was designed for ALP detection utilizing the fluorogenic response of thiamine (TH) triggered by Cu 2+ (Cu 2+ -TH system). It was found that Cu 2+ facilitated the oxidation of non-fluorescent TH to fluorescent thiochrome (TC) in an alkaline environment, while pyrophosphoric acid (PPi) interacted with Cu 2+ to form PPi-Cu 2+ chelates, preventing the oxidation of TH to fluorescent TC. Consequently, a faint fluorescence output was detected in the system. After adding ALP into this system, PPi was catalyzed to phosphate (Pi), which combined weakly with Cu 2+ . A large amount of TH was oxidized into fluorescent TC by free Cu 2+ , leading to high fluorescence. Therefore, an ALP sensing platform was constructed by utilizing the fluorogenic reaction of the Cu 2+ -TH system as the signal reporter. The assay exhibited favorable analytical performance for ALP measurement. A linear correlation was obtained between the ALP concentration (0.01–1 mU/mL) and the fluorescence intensity of the Cu 2+ -TH system, with a limit of detection as low as 0.0038 mU/mL. Additionally, the assay was further employed for analyzing ALP content in complex biological samples. This work thus advances the science of in situ fluorogenic reaction in the field of biosensing. Graphical
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
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IF 3.3
Gahlot Himanshi,Kang Sun Chul
Synergistic therapy is unwaveringly developing as a potential approach to enhance the effectiveness of anticancer medications in chemotherapy. Incorporating adjuvants alongside conventional anticancer medications has demonstrated selective cytotoxicity toward cancer cells while mitigating adverse effects such as DNA damage and systemic toxicity in normal cells. The extended use of 5-FU, a synthetic medicine used in chemotherapy, has many adverse effects, which limit its long-term use in clinical settings. Numerous investigations have demonstrated that luteolin exhibits anticancer effects and alleviates inflammation in mammalian cells. In this study, we provide the first experimental evidence of the synergistic interaction between luteolin and 5-FU in murine melanoma (B16F10) cells, compared to monotherapies (luteolin and 5-FU alone). Using MTT assay for cell viability, flow cytometry for cell cycle analysis, and Western blotting for apoptosis markers, we demonstrate that simultaneous exposure to luteolin and 5-FU speeds up oxidative stress and enhances the production of endogenous ROS. The combination therapy induced the G1-phase cell cycle arrest (65.39%) and decreased the cell viability of B16F10 cells to 34.6%. This, in turn, activates the DNA damage response and initiates the apoptotic pathway by suppressing autophagy and the DNA repair system. Further, the co-treatment of luteolin and 5-FU significantly decreases the mitochondrial membrane potential and reduces ATP production. The results indicate that the combination of luteolin and 5-FU may improve treatment efficacy while potentially decreasing the necessary dosage of 5-FU, therefore reducing its toxicity in clinical settings.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2025
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IF 3.1
Ma SongYuChen,Li YinHui,Di Yang,Liu Tao,Wang DeQi,Shi WenJing,Ling ZhiGang,Li FengSheng,Liu Jie
To enhance the safety performance and thermal stability of 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexazepane (CL-20), a core–shell composite was synthesized via in situ polymerization of aniline on polyethyleneimine (PEI)-modified CL-20. PEI modification introduced –NH 2 groups onto CL-20 surface, providing active sites for aniline polymerization and inhibiting its self-polymerization, thus forming dense polyaniline (PANI) shells with uniform thickness (about 2.8 mass%) while maintaining the high-energy crystal structure of CL-20. Compared to pure CL-20, CL-20-P@PANI showed a 15.8 °C increase in phase transition temperature (185.5 °C) and a 4 °C rise in thermal decomposition temperature (247.21 °C), attributed to the thermal barrier effect of PANI shells. The results of the mechanical sensitivity test indicate that the dense polyaniline shell layer effectively dissipates external forces, leading to a 51.43% reduction in impact sensitivity and a 20% decrease in friction sensitivity. This study provides a novel modification approach for high-energy materials by combining PEI-mediated interface engineering with in situ polymerization, ensuring both safety and energy characteristics. Graphic abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 22
·1-12
·
IF 2.8
Liu Gang,Pu Zining,Xu Limeng,Yao Cheng,Zhang Changhai,Zhang Tiandong,Chi Qingguo
Conventional polymer film capacitors suffer from significant performance degradation at high temperatures due to increased conductivity loss. This study aims to enhance the high-temperature energy storage performance of polyetherimide (PEI) films by constructing a PZT/SiO 2 heterojunction composite dielectric structure. PEI films were prepared by solution casting and then modified by magnetron sputtering to deposit PZT and SiO 2 layers. At 150 °C, the composite films exhibited a high breakdown strength of 636.7 MV/m, a discharged energy density of 4.28 J/cm 3 , and a charge–discharge efficiency exceeding 90%. The PZT/SiO 2 heterojunction effectively inhibited charge injection, reducing leakage current and improving cyclic stability with minimal degradation after 50000 charge–discharge cycles. This scalable method offers a promising approach for developing high-performance polymer-based energy storage materials suitable for high-temperature applications.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 24
·1-13
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IF 2.8
Rafiq Muhammad Nasir,Dai Zhonghua,Zheng Yuanyuan,Li Xujun,Liu Chenxi,Zhao Xin,Cong Yu,Gu Shuitao
The performance of lead-based ceramics in energy storage applications is often hindered by their low energy density and inadequate phase transition properties. The (Pb, La) (Zr, Sn, Ti)O 3 (PLZST) antiferroelectric ceramics have attracted much attention as energy storage materials. In this work, ceramics Pb 1−1.5 x La x (Zr 0.93 Sn 0.05 Ti 0.02 )O 3 at ( x = 0.015, 0.025, 0.035, and 0.045) were prepared using a solid-state method, and their dielectric and energy storage characteristics were studied. It was observed that integrating lanthanum significantly improved the perovskite structure, reducing grain size and boosting the polarization intensity. In this present study, a recoverable energy storage density ( W rec ) of 6.24 J/cm 3 was achieved under an electric field of 270 kV/cm. Moreover, these ceramics demonstrated an impressive discharge energy density ( W dis ), with 90% of the energy released within 1.29 μs. The outcomes show that PLZST is a highly effective material for advanced energy storage, pulse power applications, and paving the way for technological advancements.
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JOURNAL OF POLYMER RESEARCH
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2025
·Vol. 32
·期 9
·1-11
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IF 2.8
Liu Xiang,Deng Yuxin,Wang Ping,Tang Hongyu,Li Shuangqing,Xing Zheng
The utilization of hydrogels in the domain of flexible capacitors (FCs) and multifunctional sensors (MSs) has attained considerable prevalence. However, balancing the various properties of such versatile hydrogels remains a major challenge. In the present study, a novel xIL/PAM/SA dual-network hydrogel composed of polyacrylamide/sodium alginate (PAM/SA) and an ionic liquid (IL) 1-Butyl-3-methylimidazolium hydroxide ([BMIM][OH]) is proposed. The OH − in the IL inhibits the protonation of -NH 2 groups on the polymer chain and avoids the reduction of the number of hydrogen bonds in the system due to the formation of -NH 3+ , thus enhancing the strength of the network. The results showed that the series of tough hydrogels had high tensile strength (386.64 kPa), elongation at break (338.16%), and toughness (670.83 kJ/m 3 ). Moreover, the rich functionality such as capacitive performance (198.5 mF/cm 2 ), water retention performance (LR = 25.4%), temperature sensitivity (GF = 2.12), and strain sensitivity (GF = 4.56), make it have broad application potential. This work improves the traditional dual-network hydrogels by ionic liquids (ILs) and provides a feasible idea for the design of multi-functional tough conductive hydrogels.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2025
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IF 3
Zeng Qingbin,Wang Zhen,Guo Qianni,Song Wei,Zhao Xiuchao,Yang Yuqi,Zhou Xin
Hyperpolarized 129 Xe magnetic resonance imaging (MRI) is a powerful tool for detecting respiratory system diseases. However, 129 Xe is an inert gas and lacks specific detection capability. Entrapping xenon within molecular cages to enable specific detection is a challenging task, and numerous molecular cages have been developed and evaluated to address this challenge. Herein, we report that the aluminum-based metal-organic framework, CAU-1, can effectively entrap xenon for hyperpolarized 129 Xe MRI in aqueous solutions. This platform exhibits high water stability and good dispersibility, and shows excellent xenon entrapment capability, even at a concentration as low as 50 µg/mL. Importantly, it is responsive to pH changes across a range from 6.6 to 5.0, making it promising for monitoring the weakly acidic environment in tumors or metabolic abnormality. Furthermore, the scalable and cost-effective production of this molecular cage will facilitate future advancements in molecular imaging and chemical sensing applications.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Liu Wei,Lv Yang,Ou Honghui,Zhang Jiqiu,Ren Yuxi,Xia Mengyang,Li Yang,Li He,Ren Xiaoling,Hu Huagui,Yang Guidong
Plasma-electrochemical tandem conversion with NO x − as intermediates promises a route for renewable ammonia (NH 3 ) synthesis from air and water. However, a critical challenge lies in developing electrolyzers capable of operating efficiently at large current densities. Here, we present a scalable membrane electrode assembly electrolyzer with a full runner design (MEA-FR) that achieves efficient NH 3 production at industrial current densities. Compared to conventional serpentine runner configuration, MEA-FR leveraging forced convection within porous electrodes achieves three-order-of-magnitude enhancement in NO x − mass transfer flux. This design, meanwhile, generates strong shear forces across the porous electrode, promoting rapid detachment of O 2 bubbles at the anode and reducing overpotential losses. Notably, MEA-FR exhibits a high Faradaic efficiency of 91.8 ± 1.4% for NH 3 synthesis at 500 mA cm −2 , significantly outperforming the serpentine runner counterparts (64.9 ± 1.1%). Furthermore, a scaled-up 4 × 25 cm 2 MEA-FR stack with four modular cells is assembled with rotationally symmetric bipolar plates, delivering high NO x − conversion efficiency (>95%), high Faradaic efficiency (>91%), and long-term stability (>200 h) under industrial-relevant current densities.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Fan Rong,Guan Zeyuan,Zhou Guanghong,Yang Xi,Zhang Fei,Wu Menglong,Wang Xuecui,Liu Jian,Chen Pei,Liu Yanjun,Zhang Delin,Yin Ping,Yan Junjie
Amylopectin, the primary form of starch in plant leaves, seeds and tubers, features a tree-like architecture with branched glucose chains. Excess branches result in the formation of soluble phytoglycogen instead of starch granules. In higher plants and green algae, the debranching enzyme isoamylase ISA1 forms either homomultimer or hetero-multimer with ISA2 to facilitate branch trimming and starch granule formation, but the molecular basis remains largely unknown. In this study, we reconstitute the rice OsISA1-ISA2 complex in vitro and determine the cryo-EM structures of the OsISA1 homodimer, as well as the malto-oligosaccharide (MOS)-free and MOS-bound OsISA1-ISA2 heterocomplex. The OsISA1 dimer shows a tail-to-tail rod-like architecture, whereas the OsISA1-ISA2 complex mainly exhibits as a trimer, with OsISA2 flanking on the N-terminal segments of the dimeric OsISA1. Combined with comprehensive biochemical analyses, these structural data elucidate the organization of the ISA1-ISA2 heterocomplex in higher plants and demonstrate how ISA1 and ISA2 cooperate during amylopectin biosynthesis.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Cai Chongyang,Li Leipeng,Lv Xiaohuan,Li Huimin,Li Tao,Li Pei,Zhao Wenting,Zi Lingzhu,Feng Shiji,Fan Xuan,Zhang Hailei,Peng Dengfeng,Wang Feng,Qiu Jianrong,Yang Yanmin
Lanthanides-doped luminescent materials have gathered considerable attention due to their application potential in stress sensing, lighting and display, anti-counterfeiting technology and so forth. However, existing materials mainly cover the 380–1540 nm range, with slight extension to the UV region, impeding their applications in solar-blind imaging, background-free tracking, concealed communication, etc. To address this challenge, here we propose guidelines for far-UVC (200–230 nm) optical design. Accordingly, we achieve multi-stimulated far-UVC luminescence at ~222 nm in Pr 3+ -doped SrF 2 , stemming from the inter-configurational 4f5d → 4f 2 transition of Pr 3+ . Besides Pr 3+ , the SrF 2 host shows high tolerance to Ce 3+ , Nd 3+ , Sm 3+ , Eu 2+,3+ , Gd 3+ , Tb 3+ , Dy 3+ , Ho 3+ , Er 3+ , Tm 3+ and Yb 3+ , vastly extending the emission wavelength across the entire spectral range from 200 to 1700 nm. Particularly, these materials exhibit self-recoverable mechanoluminescence by direct mechanical excitation, along with thermally and mechanically stimulated emission after X-ray irradiation. We demonstrate that these lanthanides-doped SrF 2 crystals offer unique opportunities for high-contrast marking and structural health monitoring in complex environments.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Qian Shiwu,Wang Chenyu,Fang Changxiang,Li Meng,Qiao Yan,Hu Weihua
Understanding the extracellular electron transfer (EET) process of electroactive bacteria is of great significance. It is critical yet challenging to differentiate the partial currents from direct (DET) and mediated electron transfer (MET) pathways in the integrated EET current. Herein the EET current of model exoelectrogen is successfully disentangled by using spatiotemporally-resolved oblique-incidence reflection difference (OIRD) technique coupled with polyaniline (PANI)-based dual electrode. The PANI film serves as an electron acceptor to translate the charge information into OIRD signals, enabling mapping of EET current. Upon complete reduction of PANI, the local EET current is switched off, and the soluble mediators are forced to discharge on the nearby PANI electrode, enabling measuring of MET current. In such a way, the DET and MET currents are measured and the average currents from each bacterium are quantified. As-reported method enables successful disentangling the EET current and may offer valuable insights to related research.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-16
·
IF 5.3
Meng Jingjing,Han Lu,Li Lu,Yin Xue-bo,Zheng Jing
Manganese dioxide (MnO 2 )-based heterogeneous catalysts have gained significant attention in nanozyme research due to their exceptional oxidase-mimicking capabilities. This work reports the rational design of MnO 2 functionalized magnetic carbon nanotubes (Fe 3 O 4 @C@MnO 2 ) through a multistep synthesis strategy. The magnetic N-doped hollow carbon substrate (Fe 3 O 4 @C) was first synthesized via controlled pyrolysis of FeOOH-coated polydopamine precursors. Subsequent interfacial redox reaction with KMnO 4 enabled the in situ deposition of ultrathin MnO 2 nanosheets on the nanotube substrate, forming a triple-component coaxial architecture. This hollow structure not only facilitates dense MnO 2 deposition but also maintains robust magnetic responsiveness. The obtained Fe 3 O 4 @C@MnO 2 exhibits remarkable enzyme-mimetic capability, catalyzing TMB chromogenic conversion with 0.92-fold enhanced catalytic performance relative to pristine MnO 2 . Systematic mechanistic studies reveal that tannic acid (TA) competitively inhibits ·OH radical generation via MnO 2 -TA complexation, while the carbon interlayer enhances electron transfer efficiency. Owing to the superior enzyme-mimetic performance of Fe 3 O 4 @C@MnO 2 and their inhibition effect by TA, a sensitive colorimetric biosensing assay was fabricated, achieving a TA detection limit of 0.029 μM with a 0.096–4.82 μM linear concentration range. This work exemplifies the synergy of structural engineering and component optimization in designing multifunctional nanozymes, showing potential applications in food quality monitoring, environmental analysis, and point-of-care diagnostics. Graphical
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