CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2026
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IF 1.4
Qi Wei,Fenglan Pan,Guanghua Su,Weifang Jiang,Jiarui Li,Xiaohui Xu,Xuehui Tang,Jinfang Nie,Wenying Jin,Yun Zhang
This work initially reports a simple, instrument-free, quantitative colorimetric assay of organophosphorus pesticides based on the analyte-inhibited in-situ formation of Au nanoparticles (AuNPs) suppressing their Tyndall effect (TE). Malathion was tested as a model analyte of organophosphorus pesticides. L-ascorbic acid-2-phosphate can be dephosphorylated by alkaline phosphatase to generate L-ascorbic acid, which can subsequently reduce chloroauric acid to produce colloidal AuNPs that display a pronounced TE response under irradiation with a laser pointer. In the presence of malathion, the analyte would effectively inhibit catalytic activity of the alkaline phosphatase, preventing the AuNPs’ formation to switch off their TE. The TE is inversely related to the malathion level and can be quantitatively measured by a smartphone. The results showed that this new method enabled a linear concentration range spanning four orders of magnitude (50 nM-15 µM) for malathion quantification. The limit of detection was estimated to be as low as 1.29 nM (3σ). When it was applied to detect real samples (i.e., pond water and tap water), recoveries of malathion ranged from 94.0 to 107.3%. The developed TE-based colorimetric approach offers many advantages including simplicity, low cost, and portable smartphone readout, thus holding great promise for on-site analysis and/or household self-testing of organophosphorus pesticides like malathion.
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Journal of Future Foods
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2026
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IF 7.2
Xiaobo Zhang,Zekun Long,Wei Wang,Yafeng Jiang,Qiuyue Zheng,Jijuan Cao
Aflatoxin B1 (AFB1), the most toxic mycotoxin among aflatoxin family, which presents a risk to food safety and human health at a trace level. Herein, the self-enhanced electrochemiluminescence (ECL) emitters of Au nanoclusters-anchored g-C 3 N 4 nanosheet (Au NCs@g-C 3 N 4 ) were synthesized, in which the Au NCs as a co-reaction accelerator enhanced the ECL stability and intensity. Integrating with CRISPR/Cas12a system, the Au NCs@g-C 3 N 4 behaved as sensing substrate to construct a “off-on” switch-type ECL sensor for AFB1 detection based on ECL resonance energy transfer (ECL-RET). The presence of targets AFB1 can specifically bind its aptamer to trigger the release of the activator. Using dual aptamer-regulated activation of CRISPR-Cas12a, AFB1 can be quantified via the ECL sensor, displaying a wide detection range from 0.2 pg/mL-100 ng/mL and a detection limit of 0.08 pg/mL. Furthermore, good practicability was verified in real samples assay, providing an efficient tool for the early warning of AFB1 pollution.
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Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
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2019
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IF 2.17
Tehseen Yaseen,Hongbin Pu,Da-Wen Sun
In this study, a surface-enhanced Raman scattering (SERS) approach based on silver-coated gold nanoparticles (Au@Ag NPs) was established for rapid detection of multiple organophosphorus pesticides (triazophos and methyl-parathion) in peach fruit. The Raman enhancement of Au@Ag NPs for detecting organophosphorus pesticides was stronger than those of single Ag and Au NPs. It was revealed that core size of Au NPs was a critical parameter affecting the enhancement of Raman signals by joining two plasma resonance absorptions. The Au@Ag NPs with 26 nm Au core size and 6 nm Ag shell thickness showed significant Raman enhancement, especially by the creation of hot spots through NPs aggregation induced by connection between Au@Ag NPs and target molecules. The detection limits of triazophos and methyl-parathion in peach were 0.001 mg/kg. Good recovery (93.36 to 123.6 %) and high selectivity of the SERS activity allowed excellent precision for the detection of the triazophos and methyl-parathion in peach. Compared to earlier studies, the current approach was rapid, inexpensive and simple without lengthy sample pre-treatment. This study revealed that the proposed method could be employed for the analysis of trace contaminants such as triazophos and methyl-parathion in many food matrices.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6
Chen Yang,Limin Zhang,Zijun Ma,Junhao Gao,Yang Zhou,Bing Li,Jianbo He,Fang Li
Background The detection and quantification of glucose in human biofluids are critical for the early diagnosis and management of metabolic disorders such as hypoglycemia and diabetes. Non-invasive wearable sensors for sweat glucose monitoring have emerged as promising alternatives to traditional blood glucose testing due to their ease of use, rapid response, and portability. However, the development of stable, cost-effective, and enzyme-free wearable platforms capable of reliable sweat glucose detection remains a significant challenge. Current enzyme-based sensors often suffer from poor stability under ambient conditions, complex fabrication processes, and high costs, limiting their widespread applicability in resource-constrained settings. Results Au NPs/Cu-TCPP(Fe) nanozymes exhibiting dual peroxidase-like and glucose oxidase-like catalytic activities were synthesized and characterized. These nanozymes catalyze a cascade reaction: glucose oxidation generates H 2 O 2 , which is then decomposed to produce hydroxyl radicals that oxidize 3,3′,5,5′-tetramethylbenzidine (TMB), yielding a blue-colored product for colorimetric detection. A flexible, multi-layer hydrophilic-hydrophobic array patch was fabricated by integrating the nanozymes and TMB into a paper-based matrix. This design enables efficient in situ sweat collection, concentration, and colorimetric analysis. The patch exhibits a linear detection range of 20–200 μM with a detection limit of 12.72 μM, suitable for physiological sweat glucose levels. Selectivity tests confirmed negligible interference from common sweat components. A smartphone application was developed to enable real-time, quantitative analysis of colorimetric signals. Clinical validation on multiple volunteers across different skin locations (chest, back, forearm, forehead) showed strong correlation with reference methods (HPLC-MS, commercial glucose detection kit), with relative errors <10 %. Significance This study presents a low-cost and enzyme-free sweat glucose detection platform based on Au NPs/Cu-TCPP(Fe) nanozymes. The integration of smartphone technology enables point-of-care testing, making the platform particularly suitable for personalized health monitoring. The successful validation in human subjects demonstrates its potential for real-world applications in diabetes management and early disease detection. This work bridges the gap between nanozyme catalysis and wearable sensor technology, providing a versatile platform for non-invasive biomarker detection.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 2
·81
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IF 5.3
Zhang Haili,Peng Man,Lu Anjiang,Peng Xishun,Wang Cunwei,Zu Caizhen,Bai Zhongchen
A surface-enhanced Raman scattering (SERS) sensor of Au/Ag nanoparticles (NPs) grown on ZnO/polypyrrole@Poly(3,4-ethylene dioxythiophene)-poly(styrene sulfonate) (PPy@PEDOT:PSS) film for detecting norfloxacin (NFX) residues in milk is presented. The ZnO/PPy@PEDOT:PSS film was prepared by the electrochemical deposition method. Subsequently, Ag NPs and Au NPs were deposited on the surface of ZnO/PPy@PEDOT:PSS film to form a Au/Ag/ZnO/PPy@PEDOT:PSS film by an electrochemical deposition process and the magnetron sputtering process, respectively. Owing to the charge transfer between the SERS substrate and the NFX molecules, this SERS sensor could effectively identify low-concentration NFX molecules with a limit of detection (LOD) of 0.84 nM. Moreover, it could also recognize NFX residues in milk with recoveries of 91.2% to 108.4%. The SERS sensor of Au/Ag/ZnO/PPy@PEDOT:PSS film had a high sensitivity, excellent long-term stability, and significant potential in food safety monitoring applications. Graphical abstract
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Li Ming,Ning Li,Hongyan Zhang,Xiaobo Zou,Can Zhang
A “all-in-one” nanolabel APZ was constructed. • High Nbs coupling, dual spectral overlap, visible absorption, pH-triggered catalysis. • The three orthogonal signals enhance reliability and enable flexible applicability. • ZIF-8 “enrichment–confinement–pH-triggered collapse” synergy boosts catalysis. • Stabilize Au Pt heterostructure via ZIF-8–enabled physical isolation and protection. Conventional lateral flow immunoassay (LFIA) suffers from limited sensitivity due to single-signal readout, low antibody conjugation efficiency, and steric hindrance associated with bulky conventional antibodies. Herein, we developed a multimodal LFIA employing Au@Pt@ZIF-8 (APZ) as a multifunctional nanolabel, prepared by encapsulating flower-like Au@Pt nanoparticles (Au@Pt NPs) within a ZIF-8 framework. Benefiting from its high specific surface area and mesoporous structure, APZ significantly enhanced the coupling efficiency and stability of anti-3-phenoxybenzoic acid nanobodies (3-PBA Nbs), achieving a coupling efficiency of 94.75% under identical conditions, compared with 69.61% for Au@Pt NPs. APZ further exhibits a broad absorption spectrum, fluorescence quenching due to dual spectral overlap, and high catalytic activity arising from the intrinsic peroxidase-mimicking activity of Au@Pt, synergistically enhanced by ZIF-8 enrichment/confinement and pH-triggered framework collapse. These features enabled the integration of three orthogonal readout modes, namely aggregation-induced coloration, catalysis-induced chromogenesis, and fluorescence quenching, into a single test strip. The resulting multimodal APZ-LFIA attains limits of detection (LOD) for 3-PBA of 0.004 ng/mL, 0.001 ng/mL, and 0.0003 ng/mL, respectively. By integrating multiple complementary signals, this strategy offers a flexible and reliable platform for trace 3-PBA analysis and shows great promise for on-site food safety monitoring. Download: Download high-res image (87KB) Download: Download full-size image
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FOOD CHEMISTRY
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2023
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IF 8.8
Bingxue Hu,Hongbin Pu,Da-Wen Sun
In this study, a high-performance, stable and homogeneous [email protected] /CMC/qPCR flexible film surface-enhanced Raman scattering (SERS) substrate was constructed by synergistically stabilizing and protecting bimetallic core–shell [email protected] nanorods ( [email protected] ) with carboxymethylcellulose (CMC) and fluorescent-quantitative-polymerase-chain-reaction (qPCR) film. The network structure of CMC immobilized and aligned [email protected] through coordination of carboxyl groups with surface Ag atoms to provide intensive and stable ‘hot spots’, and the qPCR bilayer film performed as carrier and barrier to protect [email protected] from oxidation, humidity and optical damage and improved the robustness and stability. The [email protected] /CMC/qPCR film was used for in-situ extraction and SERS detection of thiabendazole on nectarine (0.24 ppm) and lemon (0.27 ppm) with low detection of limits. Furthermore, it retained 98.6% SERS performance after storage for 90 days under ambient conditions, revealing the great potential in promoting the commercialization of the SERS technique for sensitive contaminants sensing with simple fabrication procedures, homogeneity, reproducibility and long-term stability.
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BIOELECTROCHEMISTRY
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2023
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IF 5
Zheng-Zhi Yin,Zixuan Liu,Min Zhou,Xu Yang,Guojun Zheng,Hongyu Zhang,Yong Kong
Sensitive detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein (S protein) is of significant clinical importance in the diagnosis of COVID-19 pandemic. In this work, a surface molecularly imprinted (SMI) electrochemical biosensor is fabricated for the detection of SARS-CoV-2 S protein. Cu 7 S 4 -Au is used as the built-in probe and modified on the surface of a screen-printed carbon electrode (SPCE). 4-Mercaptophenylboric acid (4-MPBA) is anchored to the surface of the Cu 7 S 4 -Au through Au–SH bonds, which can be used for the immobilization of the SARS-CoV-2 S protein template through boronate ester bonds. After that, 3-aminophenylboronic acid (3-APBA) is electropolymerized on the electrode surface and used as the molecularly imprinted polymers (MIPs). The SMI electrochemical biosensor is obtained after the elution of the SARS-CoV-2 S protein template with an acidic solution by the dissociation of the boronate ester bonds, which can be utilized for sensitive detection of the SARS-CoV-2 S protein. The developed SMI electrochemical biosensor displays high specificity, reproducibility and stability, which might be a potential and promising candidate for the clinical diagnosis of COVID-19.
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CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Xuefeng Yu,Chuanhao Yan,Jinglei He,Jinlong Zhang,Hangzhou lv,Fuqiang Liu,Jianlong Wang,Junfeng Liu,Xilong Wang
To synthesize a novel magnetic nanomaterial for effective Au(III) recovery from the aqueous phase with excellent capability, superior selectivity, one-step reduction and ease of separation after loading Au(III) still remains a huge challenge. In this study, a magnetic nanomaterial (γ-Fe 2 O 3 @PDA-NAM) with a core–shell structure was synthesized for effective Au(III) recovery by sorption. The maximum Au(III) sorption capacity by γ-Fe 2 O 3 @PDA-NAM reached 2910 mg/g at pH = 6.10 ± 0.15, 44.29 times that by γ-Fe 2 O 3 (65.7 mg/g). Its Au(III) sorption efficiency was over 97.75% at very low concentrations (i.e., 5.5 × 10 -4 and 5.5 × 10 -5 mg/mL). TEM, XPS and FTIR analysis verified that excellent Au(III) recovery by γ-Fe 2 O 3 @PDA-NAM resulted from N/S-containing soft base moieties introduced to different layers. N and/or S elements at these sites provided lone pair electrons to Au(III) to form stable coordination bond. Meanwhile, the hydrogen bond between O-containing (–OH, -C=O) and N-containing (-C-N, -N(-C)-N-, -C=N) moieties on γ-Fe 2 O 3 @PDA-NAM and –OH on the main speciations of Au(III) (AuCl 3 (OH) - , AuCl 2 (OH) 2 - , and AuCl(OH) 3 - ) also greatly enhanced Au(III) recovery. About 78.03% of Au(III) was directly reduced to elemental gold by -C-S- and -N(-C)-N- group on the tertiary amine structure during sorption. More than 98.84% of Au(III) was selectively sorbed on γ-Fe 2 O 3 @PDA-NAM at pH 4.0 even though Pt(IV), Cu(II), Zn(II), Pb(II), and Ni(II) were co-present at the same concentration as that for Au(III) (0.01 mg/mL). In contrast, sorption efficiency of other interfering ions was within 1.75–26.05%. A relatively poor sorption selectivity for Pt(IV) could be acceptable because it is also a noble metal.
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Frontiers in Chemistry
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2023
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IF 5.5
Huang Liyuan,Su Ruobing,Xi Fengna
Sensitive determination of noradrenaline (NE), the pain-related neurotransmitters and hormone, in complex whole blood is of great significance. In this work, an electrochemical sensor was simply constructed on the pre-activated glassy carbon electrode (p-GCE) modified with vertically-ordered silica nanochannels thin film bearing amine groups (NH2-VMSF) and in-situ deposited Au nanoparticles (AuNPs). The simple and green electrochemical polarization was employed to pre-activate GCE to realize the stable binding of NH2-VMSF on the surface of electrode without the use of any adhesive layer. NH2-VMSF was conveniently and rapidly grown on p-GCE by electrochemically assisted self-assembly (EASA). With amine group as the anchor sites, AuNPs were in-situ electrochemically deposited on the nanochannels to improve the electrochemical signals of NE. Owing to signal amplification from gold nanoparticles, the fabricated AuNPs@NH2-VMSF/p-GCE sensor can achieve electrochemical detection of NE ranged from 50 nM to 2 μM and from 2 μM to 50 μM with a low limit of detection (LOD) of 10 nM. The constructed sensor exhibited high selectivity and can be easily regenerated and reused. Owing to the anti-fouling ability of nanochannel array, direct electroanalysis of NE in human whole blood was also realized.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
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IF 4.4
Min Chen,Yiqun Huang,Junjian Miao,Yuxia Fan,Keqiang Lai
Concerns about food safety have been arisen due to the improper use of chemicals in aquaculture. Malachite green (MG) has attracted attention because of its illegal usage and its potential negative impacts on the environment and public health. Surface-enhanced Raman scattering (SERS) platforms coupled with different SERS substrates have been employed for rapid analysis of MG residues in food. However, the most commonly used SERS substrates were non-reusable and showed limited detection sensitivity. In this study, a novel SERS substrate with a good recyclability and a high sensitivity was prepared by electrostatically assembling together a metal–organic framework material called materials of institute lavoisie-100(Fe) (MIL-100(Fe)) and Au NPs. The lowest detectable concentration of MG was 10 −13 M based on the optimal substrate. The SERS sensor was applied for the detection of the trace MG in fish pond water, which was accomplished with the correlation coefficients R 2 = 0.991–0.996 in a concentration range of 10 −6 –10 −13 M. Moreover, MIL-100(Fe)/Au was recycled at least five times, realizing a “detection to degradation”, showing great potential for food contamination monitoring due to its distinguished performance.
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ACS Sensors
·
2023
·
IF 8.9
Siqi Xie,Cheng Zhao,Jiabin Shen,Jing Wei,Haiquan Liu,Yingjie Pan,Yong Zhao,Yongheng Zhu
Listeria monocytogenes, which is abundant in environment, can lead to many kinds of serious illnesses and even death. Nowadays, indirectly detecting the metabolite biomarker of L. monocytogenes, 3-hydroxy-2-butanone, has been verified to be an effective way to evaluate the contamination of L. monocytogenes. However, this detection approach is still limited by sensitivity, selectivity, and ppb-level detection limit. Herein, low-cost and highly sensitive and selective 3-hydroxy-2-butanone sensors have been proposed based on the bimetallic AuPd decorated hierarchical flower-like WO3 nanospheres. Notably, the 1.0 wt % AuPd-WO3 based sensors displayed the highest sensitivity (Ra/Rg = 84 @ 1 ppm) at 250 °C. In addition, the sensors showed outstanding selectivity, rapid response/recovery (8/4 s @ 10 ppm), and low detection limit (100 ppb). Furthermore, the evaluation of L. monocytogenes with high sensitivity and specificity has been achieved using 1.0 wt % AuPd-WO3 based sensors. Such a marvelous sensing performance benefits from the synergistic effect of bimetallic AuPd nanoparticles, which lead to thicker electron depletion layer and increased adsorbed oxygen species. Meanwhile, the unique hierarchical nanostructure of the flower-like WO3 nanospheres benefits the gas-sensing performance. The AuPd-WO3 nanosphere-based sensors exhibit a particular and highly selective method to detect 3-hydroxy-2-butanone, foreseeing a feasible route for the rapid and nondestructive evaluation of foodborne pathogens.
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