JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Changqing Wen,Hang Su,Jiaxuan Li,Jia Zou,Mingxu Gong,Fujiang Wang,Haitao Ge
Ethnopharmacological relevance IgA nephropathy (IgAN) is characterized by the deposition of thylakoid Gd-IgA1 and progresses to kidney dysfunction and failure. Thousands of years ago, Abelmoschus manihot (L.) Medic has been used as a traditional Chinese medicine to treat kidney diseases. The total flavonoid (TFA) of Abelmoschus manihot (L.) Medic is the main active ingredient of the medicine, which is known to have therapeutic effects on kidney diseases. However, the mechanism of action is still not further explored. Aim of study We aim to reveal the targets and potential mechanisms of TFA through comprehensive proteomics and Weighted Gene Co-expression Network Analysis (WGCNA), and to provide new ideas for the treatment of IgAN. Materials and methods In this study, we constructed a model of IgA nephropathy in rats and evaluated the efficacy of TFA in the IgAN model by assessing renal function, renal Gd-IgA1/IgA and serum markers. Integrated proteomics and WGCNA analysis identified core targets associated with TFA, and target validation was performed by immunohistochemistry. Results Kidney function (Scr, PCR, BUN) and serum markers (TGF-β1, TNF-α, BAFF, MCP-1) were significantly reduced after TFA treatment. Proteomics identified 2,757 differential expressed genes (DEGs). WGCNA highlighted four key modules associated with TFA effects. Six core targets (C1QBP, CYC1, Phab, VDAC2, CDH2, Dbn1) were validated by immunohistochemistry, confirming their roles in TFA renoprotection. Conclusion Induction of rat IgAN model, proteomics and WGCNA analyses demonstrated that TFA improves renal function, reduces histopathological damage and modulates inflammation. These findings further elucidate the treatment mechanism of TFA and provide new insights into the treatment of IgA nephropathy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xinyao Su,Jia Liu,Jiarou Liu,Qiang Xue,Caixia Wang
Melia azedarach L. serves as an important source of timber and bioactive triterpenoids, notably tetracyclic limonoids and pharmacologically active pentacyclic triterpenes such as β-amyrin derivatives. Although limonoid biosynthesis has been extensively characterized, the enzymatic machinery governing pentacyclic triterpene formation remains poorly defined. Through transcriptomic mining of M. azedarach , we identified three CYP88A subfamily cytochrome P450 genes ( MaCYP88A108 , MaCYP88A164 , and MaCYP88A222 ). Functional characterization in both Nicotiana benthamiana transient expression systems and yeast microsomes demonstrated their catalytic roles as β-amyrin 11-oxidases, sequentially converting β-amyrin to 11-oxo-β-amyrin via C-11 oxidation. Intriguingly, MaCYP88A108 and MaCYP88A164 exhibited dual functionality, participating not only in pentacyclic triterpene production but also in limonoid biosynthesis, suggesting their evolutionary recruitment for triterpenoid diversification. Phylogenetic reconstruction and structural modeling revealed that these CYP88A enzymes share close homology and conserved active-site architectures with Glycyrrhiza uralensis CYP88D6 (GuCYP88D6), a characterized β-amyrin 11-oxidase, despite lacking genomic synteny. This observation supports a paradigm of functional convergence driven by structural conservation rather than shared gene lineage. Furthermore, we characterized an oxidosqualene cyclase ( MaβAS1 ) responsible for cyclizing 2,3-oxidosqualene to β-amyrin, thereby establishing the substrate pool for downstream oxidation. Our study uncovers a unique catalytic versatility in plant CYP88A P450s, demonstrating their capacity to coordinate two divergent triterpenoid branches. This functional plasticity, mediated by evolutionarily conserved structural frameworks, expands current paradigms of enzyme multifunctionality in plant specialized metabolism. The identified genes ( CYP88As and MaβAS1 ) establish critical tools for synthetic biology platforms targeting heterologous production of pharmaceutically important triterpenoids, bridging mechanistic exploration of plant secondary metabolism with biotechnological applications.
✓ 已验证
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Lin-Meng Wang,Yi-Xue Zhang,Qing Yang,Ke Li,Wu-Chao Ma,Yan-Hong Bai
In order to improve the emulsion gel properties of PSE (pale, soft, and exudative)-like chicken meat protein isolate (PPI), effect of different addition of l -lysine (L-Lys) on the emulsifying and gel properties of PPI-sunflower oil emulsion were studied by comparing control groups (pure PPI groups (PPI), only added L-Lys (Lys) and sunflower oil (SO)). Compared with control groups, adding L-Lys to the PPI emulsion resulted in a decrease in particle size and uniform distribution, the stability of emulsion was increased, and the gel exhibited higher elastic (G′), better stability and more compact microstructure was formed. Especially when 0.2 % L-Lys was added to the emulsion system, the particle size of emulsion was reduced by 76.16 %, the emulsion gel strength was increased by 286.84 %, the centrifugal loss was reduced by 51.98 % ( P < 0.05). Therefore, L-Lys can be viewed as a viable method for enhancing the functional properties of PPI
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Hui Zhang,Mengjia Xu,Ming Yang,Jun Ding,Mingquan Guo
The seeds of Cassia obtusifolia L. (Cassia seed) have long been utilized as an edible traditional Chinese medicinal herb and tea with various health benefits, including improving obesity and protecting eyesight. In this work, we developed a rapid screening method based on pancreatic lipase (PL)-functionalized magnetic composite nanoparticles coupled with ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-QTOF-MS/MS) and molecular networking analysis. This method utilized the advantages of polydopamine (PDA) and metal-organic frameworks (MOFs) functionalized magnetic core-shell structures (Fe 3 O 4 @PDA@MOFs MNPs), significantly enhancing the immobilization efficiency and performance of PL. The enzyme loading capacity was increased by 1.5-fold, and notable improvements in reusability and storage stability were also observed. By using this immobilized enzyme model in combination with UPLC-QTOF-MS/MS, 22 potential PL inhibitors were selectively screened and identified from the seeds of Cassia obtusifolia L. Molecular network analysis was innovatively introduced to improve the efficiency of compound identification by summarizing the similarity of mass spectral fragments. The inhibitory activities of three representative ligands of Cassiaside B, Biochanin A 7-glucoside and Aurantio-obtusin were further investigated. Molecular docking analysis revealed that the amino acid residue ASP249 in lipase contributes a significant role in the binding of active ligands to lipase. Overall, this proposed screening strategy, characterized by its specificity in enzyme binding and the convenience of functionalized magnetic separation, presents promising prospects for rapid and high-throughput screening of active components and drug candidates from natural resources.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.7
Dan Wang,Panpan Chen,Qin Huang,Yan Ma,Xueping Tao,Yuanmin Gong,Jiaping Cui,Yang Zhou,Zhining Xia,Qifeng Fu
The inherently low phase ratio and limited surface capacity of open-tubular capillary electrochromatography (OT-CEC) significantly restrict its efficiency in enantioseparation. To overcome this limitation, we developed a rigid-flexible composite interface strategy to construct a high-efficiency chiral ligand-exchange CEC (CLE-CEC) column. A uniform nanocrystalline UiO-66-NH 2 layer was in situ grown on the capillary inner wall to serve as a high-surface-area rigid scaffold, followed by the covalent immobilization of L-arginine (L-Arg) as a flexible chiral ligand. This composite architecture combines the structural stability of metal-organic frameworks (MOFs) with the stereochemical selectivity of amino acid ligands. The dense MOF scaffold effectively increases the specific surface area and ligand loading capacity of the stationary phase, thereby facilitating efficient Zn(II)-mediated ternary complexation for chiral recognition. Validating this design, the UiO-66-NH-L-Arg@capillary column achieved baseline separation for seven pairs of Dns-D,L-AAs, with resolution factors reaching up to 2.56. The column exhibited satisfactory repeatability and good operational stability. Furthermore, the practicability of this method was demonstrated by its successful application in the kinetic monitoring of L-alanine dehydrogenase, providing a robust micro-separation tool for precise bioanalysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 5.9
Liuyi Wei,Taoying Wu,Hao Wu,Li Wen,Bo Cui,Yunhui Cheng
Rice-derived peptides are a sustainable protein source with emerging lipid regulation functions. In this study, peptides with novel pancreatic lipase (PL) and cholesterol esterase (CE) inhibition from rice protein hydrolysates (RPHs) were identified by peptidomics. Then their alleviating effects and mechanisms on lipid accumulation in cells were investigated. 55 peptides were identified, showing significant enrichment of Phe, Pro, Arg, and Leu, with hydrophobic/polar amino acids at terminal positions. Among them, five peptides (WQ7, FR6, GF6, GA7, and GY7) exhibited potent inhibitory activity against PL and CE, with IC 50 values of 0.19-0.70 μg/mL and 0.22-0.25 μg/mL, respectively. Molecular docking studies revealed that five peptides bind stably to the active sites of PL (Phe77, Tyr114, Phe215, and His263) and CE (Ser194 and His435) through hydrogen bonding, PI-alkyl, and PI-PI interactions. Dynamic simulations demonstrated that three peptides (WQ7, FR6, and GF6) form stable complexes with both CE and PL, exhibiting strong binding affinity. In vitro study confirmed that these peptides reduced levels of TC, TG, and LDL-c while increasing HDL-c content, with WQ7 showing superior efficacy. Western blot and qPCR results indicated WQ7 regulated lipid metabolism by suppressing the PPARγ pathway and downregulating the protein expression of related factors, such as ACS, FASN, and SREBP-1c. This study showed that the WQ7, a multifunctional bioactive peptide, demonstrates direct enzyme-inhibitory activity and regulates core transcriptional pathways, providing theoretical support for utilizing rice protein in the development of lipid-lowering functional food.
✓ 已验证
产品关联
DOI
高影响因子
Reaction Kinetics Mechanisms and Catalysis
·
2026
·
IF 2.1
Sun Bai,Zhang Zhilong,Zhou Taoyou,Hong Guiyun,Wang Yun,Pan Fakang,Huang Hao,Bao Lining,Yi Mingjian,Zhu Shuguang
Zeolitic imidazolate framework-L (ZIF-L)-derived cobalt–nickel layered double hydroxide (CoNi-LDH) nanosheets are developed as a heterogeneous activator of peroxymonosulfate (PMS) for Rhodamine B (RhB) degradation. The optimized CoNi-LDH/PMS system achieves 99% RhB removal within 12 min along with an apparent rate constant ( k obs ) of 0.55 min −1 , while total organic carbon removal reaches approximately 34% at 12 min. The catalyst also retains activity toward several representative organic contaminants, during five reuse cycles, and in tap and lake water matrices. Quenching experiments, electron paramagnetic resonance, nitroblue tetrazolium probing, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy collectively indicate that PMS activation is dominated by a singlet oxygen ( 1 O 2 )-mediated non-radical pathway, with radical species making minor contributions. The results further suggest that Co/Ni redox cycling promotes PMS activation, while lattice-oxygen-related surface changes would participate in interfacial electron transfer. This study highlights a ZIF-L-derived bimetallic LDH architecture that couples rapid PMS activation with preferential 1 O 2 generation, and provides a basis for further evaluation in complex water matrices.
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Yakun Zhang,Xuelei Wang,Hao Li,Daqing Yang
As a well-known biodegradable and renewable polymer, cellulose has recently gained significant attention in the development of luminescent material, while it still remains a significant challenge to achieve multifunctional luminescent hydrogels. Here we report the construction of stimuli-responsive luminescent hydrogels through the synergistic combination of metal–ligand coordination interactions of lanthanide (Ln3+) complexes and hydrogen bonding between carboxymethyl cellulose (CMC), poly(ethylene glycol) (PEG), and pyridine-2,6-dicarboxylic acid (DPA) molecules. This molecular anchoring strategy based on the hydrogen-bonding interactions effectively suppresses the aggregation of DPA molecules and enhances the stability of Ln3+-DPA complexes in hydrogels through synergistic Ln3+-DPA coordination bond interactions, resulting in luminescent hydrogels possessing excellent luminescence performance. The dynamic double-network structure formed between CMC and PEG through hydrogen bonding interactions confers enhanced mechanical property and self-healing ability to the luminescent hydrogels. Based on changes in the luminescence brightness and emission intensity, the luminescent hydrogels can act as smart sensor for the naked-eye detection of l-ascorbic acid with good reversibility and selectivity. Furthermore, the reversible luminescence behavior of the luminescent hydrogels could be manipulated by the successive addition of l-ascorbic acid and arginine. This work may provide an interesting perspective for the design of luminescent hydrogels integrated with Ln3+ complexes and CMC-based networks.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Nanotechnology
·
2025
·Vol. 2025
·期 1
·4853716
·
IF 4.1
Nguyen Giang Nam,Nguyen Mau Thanh,Nguyen Thanh Binh,Le Van Thanh Son,Nguyen Chi Bao,Le Thi Hoa,Nguyen Duc Vu Quyen,Dinh Quang Khieu
In this study, silica nanofibers were synthesized via a ZnO-assisted hydrothermal method to develop a novel nanofertilizer with promoted plant growth potential. The aim was to fabricate one-dimensional (1D) silica nanostructures and evaluate their effectiveness when combined with chitooligosaccharide as a seed treatment agent. Structural and morphological characterization was conducted using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared (FTIR) spectroscopy. The resulting silica nanofibers exhibited diameters ranging from 10 to 100 nm and a high surface area, with ZnO and ethylenediamine serving as key complexing and structure-directing agents in the nanofiber formation. The fibers were subsequently coated with chitooligosaccharide to form a nanofiber-based organic fertilizer. Its efficacy was tested on the germination and early seedling development of peanut ( Arachis hypogaea L.) and date palm ( Phoenix dactylifera ) seeds under four treatment conditions: control, chitosan alone, uncoated silica nanofibers, and chitooligosaccharide-coated silica nanofibers. The results demonstrated that seeds treated with the coated nanofiber fertilizer showed significantly higher germination rates and greater root and leaf lengths than other treatments. Specifically, the germination rates of peanut and date palm seeds treated with COS-coated SNF were approximately 1.25 times (0.989 vs. 0.789) and 1.57 times (0.97 vs. 0.79) higher than the respective controls. Regarding root development, COS-coated SNF increased root length by 1.45 times (119 mm vs. 81.8 mm) in peanut seedlings after 7 days and by 2 times (23.1 mm vs. 11.5 mm) in date palm seedlings after 17 days. Additionally, leaf growth in date palm was enhanced by 1.97 times (228.7 mm vs. 128.6 mm) after 30 days compared to the control. These results highlight the potential of COS-coated SNF as an effective nanofertilizer to promote early plant growth and development.
✓ 已验证
产品关联
DOI
高影响因子
PHYTOTHERAPY RESEARCH
·
2023
·Vol. 37
·期 7
·3009-3024
·
IF 7.2
Yi Wang,Siyu Wang,Jingwen Xu,Yihai Wang,Limin Xiang,Xiangjiu He
Multiple drug resistance (MDR) often occurs after prolonged chemotherapy, leading to refractory tumors and cancer recurrence. In this study, we demonstrated that the total steroidal saponins from Solanum nigrum L. (SN) had broad-spectrum cytotoxic activity against various human leukemia cancer cell lines, especially in adriamycin (ADR)-sensitive and resistant K562 cell lines. Moreover, SN could effectively inhibit the expression of ABC transporter in K562/ADR cells in vivo and in vitro. In vivo, by establishing K562/ADR xenograft tumor model, we demonstrated that SN might overcome drug resistance and inhibit the proliferation of tumors by regulating autophagy. In vitro, the increased LC3 puncta, the expression of LC3-II and Beclin-1, and the decreased expression of p62/SQSTM1 in SN-treated K562/ADR and K562 cells demonstrated autophagy induced by SN. Moreover, using the autophagy inhibitors or transfecting the ATG5 shRNA, we confirmed that autophagy induced by SN was a key factor in overcoming MDR thereby promoting cell death in K562/ADR cells. More importantly, SN-induced autophagy through the mTOR signaling pathway to overcome drug resistance and ultimately induced autophagy-mediated cell death in K562/ADR cells. Taken together, our findings suggest that SN has the potential to treat multidrug-resistant leukemia.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Iranian Journal of Basic Medical Sciences
·
2023
·
IF 2.2
Junyuan Li,Gang Wang,Yehao Zhang,Xiaodi Fan,Mingjiang Yao
Objective(s): To explore the ability and underlying molecular mechanisms involved in the protective effects of Baicalin (BA) against L-Glutamate-induced mouse hippocampal neuron cell line HT-22. Materials and Methods: The cell injury model of HT-22 cells was induced by L-glutamate, and cell viability and damage were detected by CCK-8 and LDH assays. Generation of intracellular reactive oxygen species (ROS) was measured by DCFH-DA in situ fluorescence method. The SOD activity and MDA concentration in the supernatants were determined by WST-8 and colorimetric method, respectively. Furthermore, Western blot and real-time qPCR analysis were utilized to detect the expression levels of the Nrf2/HO-1 signaling pathway and NLRP3 inflammasome proteins and genes. Results: L-Glutamate exposure induced cell injuries in HT-22 cells, and the concentration of 5 mM L-Glutamate was chosen to be the modeling condition. Co-treatment with BA significantly promoted cell viability and reduced LDH release in a dose-dependent manner. In addition, BA attenuated the L-Glutamate-induced injuries by decreasing the ROS production and MDA concentration, while increasing the SOD activity. Moreover, we also found that BA treatment up-regulated the gene and protein expression of Nrf2 and HO-1, and then inhibited the expression of NLRP3. Conclusion: Our study found that BA could relieve oxidative stress damage of HT-22 cells induced by L-Glutamate, and the mechanism might be related to the activation of Nrf2/HO-1 and inhibition of NLRP3 inflammasome.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2611
·
IF 4.6
Hui Ye,Yuqin Luo,Yanjie Li,Xiangyun Wang,Hongmei He,Jinhua Jiang,Jianzhong Yu,Changpeng Zhang
A simple and sensitive method for the simultaneous quantitation of prohexadione-Ca and uniconazole in the field experiment ofOryza sativaL. andCitrus reticulataBlanco was established using solid-phase extraction (SPE) with polymer anion exchange (PAX) and Florisil followed by LC-MS/MS. The method demonstrated excellent linearity (R2> 0.999 0), trueness (recoveries between 95~105%), precision (CVs between 0.8~12%), sensitivity, and repeatability (LOQ of 0.05 and 0.01 mg/kg, respectively). Residue tests were conducted in the field at 12 representative sites in China, revealing final concentrations of prohexadione-Ca and uniconazole in brown rice, rice hull, and rice straw to be below 0.05 mg/kg, while in whole citrus fruit and citrus pulp, they were below 0.01 mg/kg. These were below the maximum residue limits specified in China. The chronic dietary risks of prohexadione-Ca and uniconazole in rice crops and citrus fruits were calculated to be 0.48% and 0.91%, respectively. Our research suggests that the chronic risk associated with the daily consumption of rice crops and citrus fruit at the recommended dosage is acceptable.Keywords:Oryza sativaL.;citrus fruit;dietary intake risk assessment;plant growth regulators
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）