JOURNAL OF MEDICINAL CHEMISTRY
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2024
·
IF 6.8
Mengzhen Gu,Xiaoqing Xu,Xiaoping Wang,Yun Wang,Yu Zhao,Xiaoxian Hu,Lu Zhu,Zhenzhong Deng,Chao Han
Histone lysine-specific demethylase 1 (LSD1) is hyperactive in breast cancer, which is associated with the metastasis of the tumor. Current irreversible LSD1 inhibitors are all synthesized by covalently binding to the flavin adenine dinucleotide cofactor, which often have side effects due to the high affinity for a variety of targets. Here, we identified isoforsythiaside (IFA), a natural phenylpropanoid glycoside isolated from Forsythia suspensa, as a novel covalent inhibitor of LSD1. The target ligand fishing technique and LC–MS/MS analysis identified that IFA could covalently bind to the Ser817 residue of LSD1 by α,β-unsaturated ketone moiety to block the amine oxidase-like domain of LSD1. Moreover, RBMS3/Twist1/MMP2, the downstream signaling pathway of LSD1, was activated after IFA treatment to inhibit the metastasis of MDA-MB-231 cells in vitro and in vivo. This study provided novel molecular templates for development of LSD1 covalence-binding inhibitor and laid a foundation for developing agents against breast carcinoma metastasis for targeting LSD1.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ying Xu,Genkuo Nie,Xiao Jiang,Hongyu Wang,Guihua Yang,Ziyi Yan,Ji-Jun Zou,Hailong Yu,Shitao Yu,Yu Liu
Metal-metal oxide catalysts are robust in selective hydrodeoxygenation of biomass derives. Herein, 1-hexanol in total yield about 76 % is firstly achieved by high selective hydrodeoxygenation of 5-hydroxymethylfurfural over Ni/MCM-41 and Pt-WO X /t-ZrO 2 . By controlling the amount ratio of Ni/Ni 2+ to 0.46, a 99.9 % yield of 2,5-tetrahydrofurandimethanol was achieved from 5-hydroxymethylfurfural by Ni/MCM-41. Subsequently high yield of 76 % of 1-hexanol was obtained by competing pathways of tandem reactions beginning at SN2 reaction at the epoxy atom of 2,5-tetrahydrofurandimethanol to enol/tautomer intermediates and ending with controllable selective hydrogenation. The reaction mechanism is explored and the reaction-oriented regulation is realized by the additional strong BrÖnsted acid sites generated by electron transfer from W to Pt and the appropriate amount of Pt 0 sites of Pt-WO X /t-ZrO 2 , where the ratios of W 5+ /W 6+ and Pt 0 /Pt 2+ are 0.31 and 4.58 respectively. This work provides a new and green method for the synthesis of 1-hexanol and expands the utilization of metal–metal oxide in making value-added products using sustainable feedstocks.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mingwei Zhang,Cheng-Bin Hong,Zhaoying Ding,Yujing Weng,Honghui Gong,Haiyong Wang,Yulong Zhang,Yuhe Liao,Haichao Liu
Hydrogenolysis of glycerol is an important reaction in biomass valorization, and requires efficient catalysts for selectively removing its hydroxyl groups to yield valuable chemicals, such as 1-propanol and 1,2-propanediol. In this work, we synthesized a porous and non-stoichiometric tungsten oxide (WO x ) by a solvothermal method, and a serious of WO x -supported Pt and Mn ((Mn)Pt/WO x ) catalysts by reduction with H 2 at different temperatures. On these catalysts, the product selectivity in the glycerol hydrogenolysis was significantly influenced by the presence of the Mn species and reduction temperatures. The catalysts after reduction with H 2 at lower temperatures favored the formation of 1-propanol, affording a yield of 88.0 % at 240 °C, while those reduced at higher temperatures facilitated the formation of 1,2-propanediol in a yield of 93.0 % under the same reaction conditions. Extensive analysis of these catalysts by in-situ and ex-situ characterizations showed that the high-temperature reduction led to the formation of MnWO 4 on the Mn/Pt/WO x catalysts and the consequent decrease in the acidic sites and increase in the basic sites, facilitating the alkali-assisted dehydrogenation of glycerol and selective formation of 1,2-propanediol. This study provides fundamental insights into the role of the W and Mn species in tuning the selectivity of the glycerol hydrogenolysis, which will be valuable for designing catalysts towards the valorization of other biomass-based feedstocks.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Cong Xu,Jiahui Guo,Baoyue Chang,Qingyun Wang,Yiming Zhang,Xianhui Chen,Wanyi Zhu,Jiage Ma,Shanshan Qian,Zhanmei Jiang,Juncai Hou
This study was conducted to develop a W/O/W emulsion encapsulated Lactobacillus plantarum 23 – 1 ( LP23 – 1 ) to significantly enhance the survival rate of LP23-1 under simulated digestion and storage conditions. The zein particles and pectin formed a complex through electrostatic interaction and hydrogen bonding. When the proportion of zein particles to pectin was 1:1, the emulsifying stability index (ESI) was 304.17 %. Additionally, when the proportion of the internal aqueous phase to the oil phase was 1:9, the polyglycerol polyricinoleate (PGPR) concentration was 5 %, the proportion of primary emulsion to the external aqueous phase was 5:5, the zein particles concentration was 4 %, and the proportion of zein particles to pectin was 1:1, the encapsulation rate was the highest at 96.27 %. Cryo-scanning electron microscopy and fluorescence microscopy confirmed the morphology of W/O/W emulsion and successful encapsulation of LP23–1 . Furthermore, compared with free LP23–1 , the W/O/W emulsion encapsulation significantly improved the survival rate of LP23–1 to 73.36 % after simulated gastrointestinal digestion and maintained a high survival rate of 78.42 % during the 35-day storage. The W/O/W emulsion was found to effectively improve the survival rate of LP23–1 during simulated digestion and storage, which has implications for the development of probiotic functional foods with elevated survival rates.
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Journal of Energy Storage
·
2025
·
IF 9.8
Tian Zhao,Shilin Peng,Jiangrong Yu,Jiayao Chen,Fuli Luo,Kar Keng Lim,Hafiz Hamsan,Nurul Syakirah Nazri
This study examines how the morphology of HKUST-1-based cellulose separators affects the performance of aqueous zinc-ion batteries. Zinc-ion batteries are attractive for large-scale energy storage due to their safety, low cost, and environmental benefits, but challenges like zinc dendrite formation and cathode instability remain. Three HKUST-1 morphologies (orthorhombic octahedral, semi-fused, and fully-fused) were synthesized and integrated with cellulose nanofibers to create composite separators. The fully-fused HKUST-1 (F-HK) separator demonstrated superior performance due to its amorphous network structure, which enhanced mechanical strength, promoted uniform zinc-ion deposition, and suppressed dendrite growth. In Zn//Cu half-cells, the F-HK separator exhibited a low nucleation overpotential of 47 mV and an average Coulombic efficiency of 99.98 % over nearly 500 cycles. In Zn//MnO 2 full cells, it retained over 80 % capacity after 400 cycles at 1 A·g −1 with near-100 % Coulombic efficiency. Additionally, the F-HK separator showed significantly enhanced mechanical strength compared to pristine cellulose separators. The findings underscore the pivotal role of morphology in enhancing separator performance and offer meaningful insights for creating high - performance MOF - based separators for aqueous zinc - ion batteries.
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CANCER RESEARCH
·
2025
·
IF 16.6
Zhang Jie,Chen Weiwu,Zhang Chen,He Qian,Wang Xudong,Han Jiayu,Gao Peng,Wang Kunyu,Xie Haoze,Gao Feng,Guo Yining,Guo Wenhao,Jiang Haofei,Le Jing,Zang Ruichen,Mo Sisi,Fan Haobo,Zhu Xiaoxiao,Jiang Xinrong,Gao Fengbin,Yu Yanlan,Ding Guoqing,Chen Yicheng
PD-1 restrains effective killing of cancer cells by the immune system and is predominantly located on the surface of T cells or other immune cells. However, cancer cells also express PD-1 to varying degrees, which is commonly associated with a poor prognosis. Here, we investigated the regulation and function of PD-1 expression in prostate cancer (PCa) and revealed the impact on the tumor microenvironment. PD-1 expression in cancer cells positively correlated with Gleason grade and metastasis but negatively correlated with CD8+ T cell infiltration in PCa patients. PCa cells secreted PD-1 in exosomes that enhanced the activity of myeloid-derived suppressor cells (MDSCs) by activating JAK/STAT3 signaling. The activated MDSCs in turn reduced the infiltration of CD8+ T cells within the tumor, promoting tumor immune evasion. The ubiquitin-specific protease USP7 induced deubiquitination and elevated the abundance of PD-1 in PCa, and USP7 inhibition sensitized PCa tumors to anti-PD-1 antibody treatment. Given the modest efficacy of current immunotherapeutic approaches for PCa, strategies to inhibit the secretion of PD-1-bearing exosomes or USP7 function may emerge as promising immunostimulatory interventions for treating PCa.
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JOURNAL OF ECONOMIC ENTOMOLOGY
·
2025
·
IF 2.4
Cao Li-Xia,Pan Xing-Run,Zhang Wan-Na,Lu Jing-Bo,Hu Zhi-Kang,Sheng Cheng-Wang,Peng Ying-Chuan
The fall armyworm (Spodoptera frugiperda), a lepidopteran pest of highly destructive, has developed resistance to organophosphate and carbamate insecticides through target-site mutations, including A201S and F290V in the ace-1 gene encoding acetylcholinesterase (AChE). Developing rapid and reliable methods to detect these mutations is crucial for monitoring resistance and guiding effective management strategies. Although S. frugiperda populations remain sensitive to the tested insecticides, the A201S and F290V mutations were identified in collected field samples. To facilitate detection, a sensitive and field-deployable loop-mediated isothermal amplification (LAMP) assay was developed, targeting these mutations within the ace-1 gene of S. frugiperda. The specificity and sensitivity of the LAMP assay was optimized for rapid detection, with reactions performed at 68 °C for 80 min. Results were visualized through color change and confirmed by gel electrophoresis. Validation using DNA from laboratory and field samples demonstrated complete agreement between the LAMP assay and DNA sequencing, affirming the accuracy and reliability of the method. The developed LAMP assay provides a simple, rapid, and effective tool for detecting the A201S and F290V mutations in the ace-1 gene of S. frugiperda. The assay’s simplicity, isothermal operation, and clear visual results make it a valuable tool for resistance monitoring and integrated pest management in agricultural settings.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
·
IF 4.8
Sensen Li,Juan Cao,Zhongjie Yang,Shaoju Jin,Lei Yang,Hao Chen
Background Chronic obstructive pulmonary disease (COPD) is a chronic respiratory disease. Licorice and dried ginger decoction (LGD) is traditional Chinese medicine prescription with multiple effects. Glycyrrhetinic acid (GA) is the main bioactive components of LGD, which has been proven to have a relieving effect on various inflammatory diseases. Siglec-1 is a cell surface sialoadhesin and has been confirmed to be overexpressed in COPD and facilitate inflammatory reaction. This study is aimed to probe the interaction between LGD, GA, and siglec-1. Methods Cigarette smoke (CS) combined with lipopolysaccharide (LPS) treatment was utilized to construct a COPD rat model. Cigarette smoke extract (CSE) was utilized to induce alveolar macrophage NR8383 to construct a COPD cell model. HE staining was applied for measuring histopathological changes of COPD rats. Enzyme-linked immunosorbent assay (ELISA), reverse transcription real-time polymerase chain reaction (RT-qPCR), and western blot were applied for testing the concentrations and expressions of proinflammatory factors. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS) analysis was utilized to determine the combination between siglec-1 and GA. JC-1 assay was utilized to evaluate mitochondrial function. Reactive oxygen species (ROS) production was tested by dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining. Results LGD treatment notably alleviated lung injury and inflammatory response in COPD rats. In CSE-induced cells, LGD treatment suppressed the contents of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-8. Sialic-acid-binding Ig-like lectin 1 (Siglec-1) expression induced by CS was decreased after LGD treatment. Furthermore, we proved that GA could target siglec-1 to regulate the inflammatory response in COPD rats and cells . Additionally, GA could reduce ROS production and alleviate mitochondrial dysfunction to suppress COPD progression. Conclusion LGD inhibits inflammation and alleviates mitochondrial dysfunction in COPD by targeting siglec-1.
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Microorganisms
·
2025
·Vol. 13
·期 7
·1698
·
IF 4.2
Yue Cheng,Qian Fu,Junjia Xu,Xinhua Niu,Lin Liu,Jiaqi Wang,Jingwen Quan,Qingyue Yu,Baoyan Chi,Haitao Li,Rongmei Liu
Prometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation is one of the main methods to solve such problems. In this study, the effects of prometryn and acetochlor-degrading strain NM-1 on soil enzymes, soil microbial communities, and physiological indexes of soybean seedlings during soil remediation were studied, and the relationship between them was discussed. The results showed that 81.54% of prometryn (50 mg·L−1) and 89.47% of acetochlor (50 mg·L−1) were degraded within 15 days after NM-1 inoculation in soil. NM-1 positively affected soil enzyme activities and soil microbial communities, and the abundance of beneficial bacteria in soil increased. More importantly, the inoculation of strain NM-1 under prometryn and acetochlor stress significantly increased plant height, root length, root volume, water content, chlorophyll concentration, and root activity of soybean. The results of these studies showed that the NM-1 strain showed significant potential in bioremediation in order to provide technical support for solving the problem of prometryn and acetochlor pollution.
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Catalysts
·
2025
·Vol. 15
·期 4
·390
·
IF 4
Zezheng Bing,Yuanyuan Gao,Zhongyi Liu,Qiaoyun Liu
Biomass, as a renewable carbon resource, holds broad application prospects. Among various bio-based platform molecules, furan derivatives play a significant role in green chemical production. Notably, the conversion of 2-methylfuran (2-MF) to 3-acetyl-1-propanol (3-AP) over bifunctional catalysts has attracted considerable interest. In this study, a Pd@PHZSM-5 catalyst was prepared by encapsulating Pd nanoparticles within P-doped HZSM-5 for 2-MF conversion. The encapsulation improved Pd dispersion and metal–acid synergy, enhancing both catalytic activity and 3-AP selectivity. Additionally, phosphorus doping increased HZSM-5 crystallinity, resulting in excellent stability. This work provides a feasible strategy for optimizing metal–acid cooperation, offering theoretical guidance for bifunctional catalysis and biomass valorization.
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BIOTECHNOLOGY AND BIOENGINEERING
·
2025
·
IF 3.6
Wenzhe Ma,Wanqing Wei,Jian Wen,Cong Gao,Guipeng Hu,Xiaomin Li,Jia Liu,Jing Wu,Wei Song
The synthesis of tertiary amides is crucial in pharmaceutical manufacturing, but achieving this efficiently through enzymatic methods remains challenging. Here, we describe the screening, evolution, and application of a lactamase for the synthesis of 2-(2-oxopyrrolidin-1-yl)-butanoic acid (2-OYBA) in aqueous phase. Through database mining, we identified a lactamase from Paraburkholderia xenovorans ( Px AmpC) with the highest amide synthesis activity in this study at 31.3 U/g. Using protein crystallization, molecular dynamics simulations, and quantum mechanics calculations, we proposed and verified three potential synthesis mechanisms for 2-OYBA. We identified a mechanism for synthesis of 2-OYBA involving adenylation and lactamization, with the carboxyl group in the intermediate 2-2 (INT2-2) acting as a proton shuttle. Guided by this mechanism, rational design efforts enhanced amide synthesis activity by 16.6-fold. The Px AmpC mutants also exhibited a broader substrate scope for amide bond synthesis compared to the wild type. Our findings advance the green synthesis of complex amide compounds.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiawei Ruan,Xiaoyi Zhang,Wanting Zhang,Xinzi Wu,Qian Liu,Zhen Song,Lifang Chen,Zhiwen Qi
The direct synthesis of dimethyl carbonate (DMC) from CO 2 is a promising route for CO 2 utilization, however it is constrained by high CO 2 activation barrier and inherent thermodynamic limitations. Herein, we developed a reactive extraction strategy to intensify the reaction through 1-alkyl-3-methylimidazolium triazolide ionic liquids (ILs) (CnMImTz, n = 2, 4, 6, and 8) by modulating IL polarity. The absorption and activation mechanism of CO 2 in ILs was demonstrated, and subsequent transformation into DMC can be achieved under mild conditions. Remarkably, C2MImTz achieves the highest DMC yield (9.4 % after 12 h at 25 °C), revealing chain-length dependent catalytic efficiency of IL analogs at both kinetics- and thermodynamic-controlled stages. Phase analyses, binding energy calculations, and molecular dynamics simulations revealed that the highly polar C2MImTz preferentially concentrate CH 3 OH and H 2 O, while spontaneously segregating weakly polar DMC into a separate phase. The enhanced CH 3 OH concentration and diffusion coefficients promote the mass transfer and reaction kinetics. Moreover, the segregation of DMC can effectively shift thermodynamic equilibrium toward DMC formation. Therefore, the integrated absorption-conversion-separation process within a single functionalized IL presents a scalable platform for CO 2 capture and utilization.
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