ANALYTICAL SCIENCES
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2023
·
IF 1.6
Zhang Miaomiao,Wei Xuanwen,Bai Ligai,Liu Haiyan
In present work, a method for enrichment, purification, and content determination of oleanolic acid (OA) in medicinal plants was established based on on-line solid phase extraction (SPE). A metal organic frameworks–porous organic polymer monolith (MOF–POPM) was prepared with functionalized UiO-66-(OH) 2 as monomer and was used as SPE column for online enrichment and purification of OA. The ratio of adsorbent, enriching and eluting solvent, mobile phase pH, and flow rate had been systematically investigated. Under the optimum conditions, the linear range of OA was 0.59–2500 μg/mL with r = 0.9996. The limit of detection (LOD) was 0.18 μg/mL and the limit of quantification (LOQ) was 0.59 μg/mL. The intra-day relative standard deviations (RSDs) and inter-day RSDs of retention time and peak area were less than 0.3% and 1.3%, respectively. The average recoveries of OA in medicinal plants samples ranged from 87.7 to 104.6%. The results demonstrated that the online system was reliable and accurate for enrichment, purification, and content determination of OA in medicinal plants. Graphical abstract
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Nano Research
·
2023
·
IF 9.9
Xi Guangshuai,Zhang Dongzhi,Tang Mingcong,Zhang Hao,Sun Yuehang,Zhang Yubiao,Cai Haolin,Xia Hui,Zhou Dandi
In recent years, multi-modal flexible tactile sensors have become an important direction in the development of electronic skin because of their excellent sensitivity, flexibility and wearable properties. In this work, a humidity-pressure multi-modal flexible sensor based on polypyrrole (PPy)/Ti 3 C 2 T x sensitive film packaged with porous polydimethylsiloxane (PDMS) is investigated by combining the sensitive structure generation mechanism of in situ polymerization to achieve the simultaneous detection of humidity and pressure, which has a sensitivity of 89,113.4 Ω/% RH in a large humidity range of 0%–97% RH, and response/recovery time of 2.5/1.9 s. The tactile pressure sensing has a high sensitivity, a fast response of 67/52 ms, and a wide detection limit. The device also has excellent performance in terms of stability and repeatability, making it promising for respiratory pattern and motion detection. This work provides a new solution to address the construction of multi-modal tactile sensors with potential applications in the fields of medical health, epidemic prevention.
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Journal of Analysis and Testing
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2023
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IF 4.7
Yang Jingxuan,Guo Wenjun,Xu Xiaohang,Zhao Liang,Xu Yajuan,Wang Yang
Fatty acids (FAs) are an important class of functional small molecules and participate in a variety of life biological processes. This experiment proposed a liquid chromatography–quadrupole-Qrbitrap mass spectrometry (LC–Q-Orbitrap MS) method to perform FA profiling under parallel reaction monitoring (PRM) acquisition mode. The FA was derivatized by 2-dimethylaminoethylamine (DMED) to increase the ionization efficiency and provide the characterized fragment pattern. The mass spectra obtained from full scan, multiple ion monitoring (MIM), and PRM were compared. The results showed that the protonated ion of FA + DMED–H 2 O was detected and the neutral loss of 45.06 Da was observed in the tandem mass spectrum. The PRM method provided the highest selectivity and sensitivity for FA detection with the help of accurate mass weight and specific neutral loss-based fragments. The method validation was performed using the FA standards and pooled quality control serum sample, which showed that the established method had good repeatability, stability, and linearity. Finally, the developed method was successfully applied to analyze the rat serum and brain tissue samples for the drug efficacy evaluation of gross saponin of Tribulus terrestris L. fruit (GSTTF) against middle cerebral artery occlusion (MCAO)-induced ischemic stroke. This method has extensive practicability and great potential in the detection of FA, especially for the analysis of samples with complex matrices.
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Nuclear Science and Techniques
·
2023
·Vol. 34
·期 12
·1-10
·
IF 2.8
Li Zhen-Jiang,Qi Cheng-Hang,Li Bei-Ning,Yang Shu-Min,Zhao Jun,Lei Zhi-Di,Zhu Shi-Jie,Shi Hao,Wang Lu,Wu Yan-Qing,Tai Ren-Zhong
Evaluating the comprehensive characteristics of extreme ultraviolet (EUV) photoresists is crucial for their application in EUV lithography, a key process in modern technology. This paper highlights the capabilities of the Shanghai Synchrotron Radiation Facility (SSRF) 08U1B beamline in advancing this field. Specifically, it demonstrates how this beamline can create fringe patterns with a 15-nm half-pitch on a resist using synchrotron-based EUV lithography. This achievement is vital for evaluating EUV photoresists at the advanced 5-nm node. We provide a detailed introduction to the methods and experimental setup used at the SSRF 08U1B beamline to assess an EUV photoresist. A significant part of this research involved the fabrication of high-resolution hydrogen silsesquioxane mask gratings. These gratings, with an aspect ratio of approximately 3, were created using electron beam lithography on an innovative mask framework. This framework was crucial in eliminating the impact of zeroth-order light on interference patterns. The proposed framework propose offers a new approach to mask fabrication, particularly beneficial for achromatic Talbot lithography and multicoherent-beam interference applications.
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ANALYTICAL SCIENCES
·
2023
·
IF 1.6
Xi Huai,Liang Xiaolin,Huang Guidan,Liang Jinhua,Li Dandan,Wen Qilin,Zhang Yun,Xiao Xiaofen,Zhu Wenyuan
The rapid and accurate detection of miRNAs is of great significance for early diagnosis and treatment of cancer. Hence, a novel enzyme-free and label-free electrochemical biosensor based on bio-barcode amplification for detecting miRNAs was presented. Sandwich structures constructed of magnetic nanoparticles modified with DNA probes, gold nanoparticles with numerous barcoded DNA strands that hybridized with target miRNAs were fabricated as the amplifier. The released barcoded DNA strands then acted as the secondary targets and triggered the electrochemical sensor with a significant electrochemical response. A highly sensitive (detection limit of 0.24 fM) and selective electrochemical miRNA detection was realized, which has great potential for application in miRNA-related clinical diagnosis and biochemical research. Graphical abstract A novel biosensor based on bio-barcode amplification for the electrochemical detection of miR-7a was successfully developed.
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VETERINARY RESEARCH
·
2023
·Vol. 54
·期 1
·1-12
·
IF 4.4
Fu Shaodong,Wang Zhenglei,Han Xiangan,Xu Yuanyuan,Miao Jinfeng
Macrophages play a pivotal role in the inflammatory response to the zoonotic pathogen E. coli, responsible for causing enteric infections. While considerable research has been conducted to comprehend the pathogenesis of this disease, scant attention devoted to host-derived H2S. Herein, we reported that E. coli infection enhanced the expression of CSE in macrophages, accompanied by a significantly increased inflammatory response. This process may be mediated by the involvement of excessive autophagy. Inhibition of AMPK or autophagy with pharmacological inhibitors could alleviate the inflammation. Additionally, cell model showed that the mRNA expression of classic inflammatory factors (Il-1β, Il-6), macrophage polarization markers (iNOS, Arg1) and ROS production was significantly down-regulated after employing CSE specific inhibitor PAG. And PAG is capable of inhibiting excessive autophagy through the LKB1-AMPK-ULK1 axis. Interestingly, exogenous H2S could suppress inflammation response. Our study emphasizes the importance of CSE in regulating the macrophage-mediated response to E. coli. Increased CSE in macrophages leads to excessive inflammation, which should be considered a new target for drug development to treat intestinal infection.
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Nano Research
·
2023
·
IF 9.9
Huang Tao,Li Ke,Lei Jinyu,Niu Quan,Peng Hui,Zou Bingsuo
Sb-based organic–inorganic hybrid metal halides (OIHMHs) with [SbCl 5 ] 2− units have been widely reported due to high photoluminescence quantum yield (PLQY) and occasional multiple self-trapped exciton (STE) emission bands mainly out of singlet and triplet states, and their multi-band emission is important in white light-emitting diode (WLED). However, not all these OIHMH compounds can produce both emissions out of singlet STE and triplet STE at room temperature simultaneously. It is crucial to consider how the singlet STE generates and retains to emit light at room temperature for this material’s design and application. Herein, a strategy is proposed that can significantly lift Sb halide PLQY by synthesizing two Sb-based OIHMHs using organic amine cations of different-sized and -quantity, which modulate the distance of neighboring emission centers. Therein, the occurrence of singlet STE emission is found to be closely related to the distance of [SbCl 5 ] 2− units and local unit distortion in the lattice. The larger distance can produce smaller local distortions, favoring the formation of the singlet STE emission band at higher energy. This is the first work to reveal the relationship between the local structure and the origin of the singlet STE emission band, providing new insights into the modulation of the Sb-based OIHMH’s emission.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2023
·
IF 2.5
Feng Chenxu,Wang Xiaoyu,Cong Qianmin,Sun Zizhan,Cai Zhengyu,Fan Chuangang,Pei Lizhai
A facile hydrothermal approach was applied for synthesizing single crystalline Sm vanadate nanowires without any surfactants, and the formation mechanism of the Sm vanadate nanowires was analyzed in detail. The Sm vanadate nanowires possess a tetragonal SmVO 4 phase, smooth surface, length of tens of micrometers, and a diameter of 30–100 nm, respectively. The Sm vanadate nanowires can be applied as the electrode materials with excellent electron transfer interface, exhibiting good electrocatalytic performance for Co 2+ detection by square wave voltammetry (SWV) technique. A well-defined potential at + 0.60 V exists at the Sm vanadate nanowire–modified electrode in KCl electrolyte with 1 mM Co 2+ . Electrolyte pH value, deposition time, deposition potential, and standing time are optimized to be pH = 6, 90 s, − 1.5 V, and 60 s, respectively. Sm vanadate nanowire–modified electrode indicates a linear detection range of 0.01–1000 μM, detection limit of 0.23 nM, good stability, reproducibility, and selectivity for Co 2+ detection. The Sm vanadate nanowires provide promising electrode materials and are applicable for the detection of Co 2+ in real water environment.
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MOLECULAR NEUROBIOLOGY
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2023
·
IF 5.1
Wu Yuhan,Zhu Zhanpeng,Lan Tian,Li Shuhan,Li Ye,Wang Changmin,Feng Yabo,Mao Xueqin,Yu Shuyan
Depression is a common psychological disease with high morbidity and mortality. Recently, the involvement of synaptic plasticity in the pathogenesis of depression has shed light on the direction of developing novel antidepressants. Levomilnacipran is a newly approved medication for the treatment of adult major depressive disorder. However, the detailed mechanisms underlying its antidepressant-like effects have yet to be illuminated. In this study, we aimed to investigate the role of levomilnacipran in regulating synaptic plasticity and explore the possible molecular mechanisms of its antidepressant effects using a rat model of depression induced by lipopolysaccharide (LPS). The results demonstrated that levomilnacipran (30 mg/kg, i.p.) significantly ameliorated depression-like behaviors in rats, alleviated the dysregulation of synaptic plasticity, and suppressed neuroinflammation within hippocampus induced by LPS-treatment. Levomilnacipran increased the expression of postsynaptic dense 95 (PSD-95) and synaptophysin (Syn) and reversed the imbalance between pro- and anti-inflammatory cytokines within hippocampus of depressed rats. Additionally, levomilnacipran elevated expression level of brain-derived neurotrophic factor (BDNF), accompanied by increased tyrosine kinase B (TrkB), phosphorylated phosphatidylinositol 3-kinase (PI3K), phosphorylated protein kinase B (p-Akt), and phosphorylated mammalian target of rapamycin (p-mTOR). Taken together, these results suggest that levomilnacipran may exert antidepressant effects via upregulating BDNF/TrkB mediated PI3K/Akt/mTOR signaling pathway to improve synaptic plasticity. These findings reveal potential mechanisms for the antidepressant effects of levomilnacipran and offer new insights into the treatments for depression. Graphical Abstract
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CHEMICAL PAPERS
·
2023
·
IF 2.2
Liu Wenqiang,Li Jiangxin,Xie Yuheng,Wang Yajuan,Zhang Juan
Dimethyl carbonate (DMC) is widely used and nontoxic and can replace more dangerous materials such as dimethyl sulfate or methyl chloride. Spherical particle catalysts (PILs-XSS) were synthesized by suspension polymerization, which was characterized by SEM–EDS, FT-IR, TG, and BET-BJH. The characterization results showed that PILs-XSS had a specific core structure, a certain pore size (14.3 nm), and surface area size (71.5m 2 /g) and remained stable at 293.4 °C. The catalyst performance was evaluated by a one-pot synthesis of dimethyl carbonate. The process conditions were optimized in detail. The optimal reaction conditions were as follows: the dosage of PILs-XSS was 2.5 wt %, the CO 2 pressure was 1.5 MPa, the reaction temperature was 100 °C, the reaction time was 4 h, the molar ratio of methanol to propylene oxide (PO) was 3: 1, and the dosage of cocatalyst Na 2 CO 3 was 3 wt %. Under the optimum conditions, the conversion of PO was up to 98.8% and the yield of DMC reached 53.7%. In addition, the combined catalyst PILs-Na 2 CO 3 was easily recovered by filtration in the reaction system. After five times of repeated use, the activity and the shape of the catalyst were almost unchanged, which meant that the catalyst had amazing mechanical strength. This study found that the transesterification catalyst Na 2 CO 3 not only had good transesterification efficiency but also promoted the reaction of CO 2 . Graphical abstract
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EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
·
2023
·Vol. 48
·期 4
·363-376
·
IF 1.9
Zhuang Yu,Wang Yuxiao,Li Ning,Meng Haitao,Li Zhiyu,Luo Jianguang,Qiu Zhixia
Background and ObjectivesThe oral bioavailability of withangulatin A (WA) is low and may undergo first-pass metabolism because of the presence of two esters bonds. This study aimed to identify the hydrolysis behavior and mechanism of WA, thus enriching its structure-pharmacokinetic relationship.MethodsThe in vivo pharmacokinetic studies of WA in rats were first investigated, followed by in vitro assays including metabolic stability, phenotyping identification and metabolic kinetics assays. After screening out the responsible enzymes with higher catalytic capacity, molecular docking study was performed to demonstrate the interaction mode between WA and metabolic enzymes. Then, metabolites in human serum albumin (HSA) were identified by LC-TOF-MS/MS.ResultsIn rats, the oral bioavailability of WA was only 2.83%. In vitro, WA was hydrolyzed in both rat and human plasma and could not be inhibited by selective esterase inhibitors. Physiologic concentration of HSA not recombinant human carboxylesterases (rhCES) could significantly hydrolyze WA, and it had a similar hydrolytic capacity with human plasma to WA. Furthermore, WA could stably bind to HSA by forming hydrogen bonds with Lys199 and Arg410, accompanied by the metabolic reaction of the lactone ring opening.ConclusionThe study showed that WA underwent obvious hydrolysis in rat and human plasma, which implied a strong first-pass effect. Serum albumin rather than common esterases primarily contributed to the hydrolytic metabolism of WA in plasma.
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MRS Communications
·
2023
·Vol. 13
·期 4
·538-545
·
IF 1.9
Yin Jinkun,Chu Yaoqing,Tan Lianjiang
Cu/N co-doped TiO 2 nanopowder was prepared with a facile sol–gel method. In the absence of stabilizing agents, the co-doped nanopowder with nanoscaled particle sizes was obtained. Doping didn’t change the anatase crystal structure of TiO 2 . Cuprite Cu 2 Cl(OH) 3 formed in the co-doped nanopowder. Based on ultraviolet–visible (UV–vis) spectroscopy and X-ray photoelectron spectroscopy (XPS) data, the mechanisms of significantly improved photocatalytic activity of the co-doped nanopowder were discussed. The antibacterial evaluation and cytotoxicity test suggested the nanopowder inhibited bacteria significantly under visible light and had ultralow cytotoxicity. This work provided a promising antibacterial agent with potential biomedical applications.
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危险品化学品经营许可证（带存储）