EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2023
·
IF 4.9
Yue Zhang,Wenxiu Pan,Dequan Wang,Han Wang,Yanting Hou,Meijuan Zou,Hongyu Piao
l -ascorbic acid （Vitamin C, VC） is the most abundant antioxidant in human skin. But its poor penetration into the skin and unstability limit the application. The aim of the study was to promote the topical skin permeation and retention of VC, increase the stability as well as effectiveness by a novel solid in oil nanodispersion. In the nanodispersions system, nano-sized particles of hydrophilic molecules are dispersed in an oil vehicle with the assistance of hydrophobic surfactants. The optimized formula composed of O170 and S1570 (12.5:1, w/w ) showed high EE% of 98% and good stability. FTIR analysis confirmed that there may be hydrogen bond between VC and surfactants. The results of DSC, and XRD revealed that the drug was successfully encapsulated in the surfactants, which maintained the stability of drug. By analyzing and fitting the release data in vitro , the drug release mechanism of SONDs was predicted as a multi-dynamic model. Skin permeation of VC was improved 3.43-fold for SONDs compared with VC aqueous solution, highlighting that the lipophilicity and nano size of the carrier more easily penetrated into the skin. Finally, the photoaging study revealed that topical application of VC-SONDs provided the highest skin protection compared UV and VC aqueous solution treated group which was evident by the normal thick epidermal morphology, no obvious melanocytes and the densely arranged dermal elastic fibers. These results demonstrated that the solid-in-oil nanodispersions may be a potential transdermal delivery system for hydrophilic bioactive ingredients.
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CARBOHYDRATE POLYMERS
·
2023
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IF 11.2
Chunwang Hu,Hongliang Wei,Bingyan Hua,Yaqi Zhang,Gang Wang,Tao Guo
A thermal/pH-sensitive interpenetrating network (IPN) hydrogel was prepared facilely from starch and poly(α- l -lysine) through amino-anhydride and azide-alkyne double-click reactions in one pot. The synthesized polymers and hydrogels were systematically characterized using different analytical techniques such as Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), scanning electron microscope (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and rheometer. The preparation conditions of the IPN hydrogel were optimized via one-factor experiments. Experimental results indicated the IPN hydrogel possessed pH and temperature sensitivity. Effect of different parameters (pH, contact time, adsorbent dosage, initial concentration, ionic strength , and temperature) on adsorption behavior were investigated in monocomponent system with cationic methylene blue (MB) and anionic Eosin Y (EY) as model pollutants. The results indicated that the adsorption process of the IPN hydrogel for MB and EY followed pseudo-second-order kinetics. The adsorption data for MB and EY fitted well with the Langmuir isotherm model, indicating monolayer chemisorption. The good adsorption performance was due to various active functional groups (–COOH, –OH, –NH 2 , etc.) in the IPN hydrogel. The strategy described here opens up a new way for preparing IPN hydrogel. The as-prepared hydrogel exhibits potential application and bright prospects as an adsorbent in wastewater treatment.
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Journal of Cleaner Production
·
2023
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IF 11.1
Dong Wang,Yumeng Wang,Luheng Jing,Lei Wang,Tonghui Zhang,Xuefen Wang
In this work, a flower-like Fe(OH) 3 @Co 2 (OH) 2 CO 3 catalyst was prepared via the confinement effects provided by L-phenylalanine, which favored the heterostructure construction of active iron centers (Fe(OH) 3 ) anchored on the Co 2 (OH) 2 CO 3 nanosheet supports. Benefiting from the created electron-transfer interface and dual metal active sites, the limitation of redox cycling was broken and exhibited highly efficient catalytic activities , the optimal Fe(OH) 3 @Co 2 (OH) 2 CO 3 catalyst showed comparable catalytic performance for the degradation of dye (degradation degree of 99% methylene blue and orange II within 18 min). In addition, the prepared catalysts were employed for the efficient removal of antibiotics (tetracycline (98.9%), norfloxacin (92.5%) and doxycycline hyclate (90.2%). Besides, the preferred nanofibrous composite membranes prepared by combination of nanofibrous membranes (NFMs) with Fe(OH) 3 @Co 2 (OH) 2 CO 3 catalysts possessed considerable potential for large-scale MB removal (treatment capacity of 238.5 L m −2 and permeability of 191.8 L m −2 h − 1 driven by gravity) and stability (182.7 L m −2 of treatment capacity was achieved in the third run). The strategy of construction of rapid interface-redox on the heterogenous catalysts was designed to enhance catalytic performance, which provided a promising chance for the efficiently large-scale removal of dyes and antibiotics.
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REACTIVE & FUNCTIONAL POLYMERS
·
2023
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IF 5.1
Peng Zhou,Xiangzhou Li,Jun Zhou,Wensheng Li,Liqun Shen
A molecularly imprinted polymer (MIPs) was prepared based on a computer-aided design, which was used to selectively enrich and purify synephrine (SYN) from the extract waste liquid of Citrus aurantium L. The suitable functional monomers , cross-linkers and reaction systems for preparing MIPs were quickly screened with density functional theory (DFT) calculation to effectively avoid the design blindness of molecularly imprinted material. The synephrine molecularly imprinted polymer (SYN-MIPs) with imprinted holes and magnetic properties were prepared with nano Fe 3 O 4 as the core, and the structure, morphology, magnetic property and adsorption behavior were studied and the interaction mechanism of the adsorption process was clarified by DFT calculations. The saturated adsorption of SYN-MIPs for SYN was as high as 47.38–53.68 mg/g within 298 K to 308 K. The adsorption behavior of SYN-MIPs for SYN was well fit for the Freundlich adsorption and the pseudo-second-order kinetic model. It was worth mentioning that SYN-MIPs showed distinguished re-usability and specific recognition. The imprinting factor and selection factor to SYN were 3.13 and 2.43, respectively. The selective enrichment and purification of SYN from the extract waste liquid of Citrus aurantium L. achieved satisfactirily, and the purity of SYN enriched to 93.73% while the concentration of SYN was only 1.73% in the extract waste liquid. The study provides not only an efficient adsorption separation polymer materials but also a practical for the enrichment and purification process of SYN from the extract waste liquid of citrus plants.
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Nutrients
·
2022
·Vol. 14
·期 19
·3980
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IF 6.706
Shan-Shan Zhang,Yu-Fei Hou,Shao-Jing Liu,Sen Guo,Chi-Tang Ho,Nai-Sheng Bai
Forty compounds were isolated and characterized fromA. tenuissimumflower. Among them, twelve flavonoids showed higher α−glucosidase inhibition activities in vitro than acarbose, especially kaempferol. The molecular docking results showed that the binding of kaempferol to α−glucosidase (GAA) could reduce the hydrolysis of substrates by GAA and reduce the glucose produced by hydrolysis, thus exhibiting α−glucosidase inhibition activities. The in vivo experiment results showed that flavonoids−richA. tenuissimumflower could decrease blood glucose and reduce lipid accumulation. The protein expression levels of RAC−alpha serine/threonine−protein kinase (AKT1), peroxisome proliferator activated receptor gamma (PPARG), and prostaglandin G/H synthase 2 (PTGS2) in liver tissue were increased. In addition, the Firmicutes/Bacteroidetes (F/B) ratio was increased, the level of gut probioticsBifidobacteriumwas increased, and the levels ofEnterobacteriaceaeandStaphylococcuswere decreased. The carbohydrate metabolism, lipid metabolism, and other pathways related to type 2 diabetes mellitus were activated. This study indicating flavonoids−richA. tenuissimumflower could improve glycolipid metabolic disorders and inflammation in diabetic mice by modulating the protein expression and gut microbiota.Keywords:A. tenuissimumflower flavonoids;network pharmacology;molecular docking;gut microbiota;type 2 diabetes mellitus
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FOOD CHEMISTRY
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2022
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IF 9.231
Obafemi Ibitayo Obajemihi,Okon Johnson Esua,Jun-Hu Cheng,Da-Wen Sun
The effects of pretreatments using plasma functionalized water (PW), osmodehydration (OD), and combined plasma functionalized water and osmodehydration (PO) on the drying characteristics, physicochemical and bioactive components of tomato slices during hot air drying at an air temperature of 55 °C and velocity of 1.5 m/s were evaluated. Results revealed that PW pretreatment led to an increase in lycopene compared to fresh samples, and shortened drying time, improved ascorbic acids, TPC, TFC, acidity, rehydration, porosity and hue, but reduced TSS, compared to dried control samples, while OD resulted in lycopene degradation during pretreatment, and prolonged drying time, increased TSS, but lowered acidity, rehydration, porosity and hue, compared to dried control samples. On the other hand, PO was found to overcome the shortcomings of OD with enhancement in the lycopene during pretreatment, and showed accelerated moisture transfer, improved bioactive, acidity, porosity, rehydration, hue and texture, but decreased TSS, when compared to dried control samples. Overall, the results showed the promising potential of PW and PO pretreatments for enhancing drying efficiency and product quality for the food industry.
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Agriculture-Basel
·
2022
·Vol. 12
·期 7
·988
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IF 3.408
Yaojun Liu,Wenqiang Shen,Tao Liu,Rainer Mosenthin,Yinghui Bao,Peng Chen,Wenbo Hao,Lihong Zhao,Jianyun Zhang,Cheng Ji,Qiugang Ma
(1) Background: L-carnosine (β-alanyl-L-histidine), a natural dipeptide, exists at relatively high concentrations in skeletal muscles, and has been shown to protect cells from adverse conditions due to its antioxidant, anti-aging, anti-glycation, and buffering properties. Satellite cells (SCs), residing on the myofiber surface, are crucial for muscle post-growth and regeneration. However, the effects of L-carnosine on muscle development of pigs in vivo, on proliferation and growth of SCs in vitro, and the relationship between SCs and muscle development have not yet been investigated. (2) Methods: The objective of this study was to assess the effect of dietary L-carnosine on growth performance andlongissimus dorsimuscle development of pigs in vivo, and to elaborate its molecular mechanisms in vitro using L-carnosine-treated SCs. (3) Results: It was shown that L-carnosine supplementation (0.2 and 2 mM) increased (p< 0.05) SC proliferation and cell percentage in the synthesis (S) phase and decreased cell percentage in the resting (G0)/first gap (G1)/phase. Moreover, average daily gain (ADG) of pigs fed diets containing 0.1% of L-carnosine was higher (p< 0.05) than that of pigs fed diets without L-carnosine, and thelongissimus dorsimuscle weight of pigs assigned to the L-carnosine treatments was 7.95% higher compared to control pigs. Both in thelongissimus dorsimuscle and cultured SCs of pigs, the Akt/mTOR/S6K signaling pathway was activated (p< 0.05), suggesting that L-carnosine improved muscle growth and SC proliferation of pigs. (4) Conclusions: Considering the important role of SCs in post-natal muscle growth, there is evidence that L-carnosine may improve muscle growth of pigs through promoting SC proliferation via activating the Akt/mTOR/S6K signaling pathway.Keywords:L-carnosine;satellite cells;proliferation;mTOR signaling pathway;muscle growth
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3 Biotech
·
2022
·Vol. 12
·期 9
·1-15
·
IF 2.893
Weng Chunyue,Mi Zheyan,Li Meijing,Qin Haibin,Hu Zhongce,Liu Zhiqiang,Zheng Yuguo,Wang Yuanshan
To improve S-Adenosyl-L-methionine (a compound with important physiological functions, SAM) production, atmospheric and room temperature plasma and ultraviolet-LiCl mutagenesis were carried out with Saccharomyces cerevisiae strain ZY 1–5. The mutants were screened with ethionine, L-methionine, nystatin and cordycepin as screening agents. Adaptive evolution of a positive mutant UV6-69 was further performed by droplet microfluidics cultivation with ethionine as screening pressure. After adaptation, mutant T11-1 was obtained. Its SAM titer in shake flask fermentation reached 1.31 g/L, which was 191% higher than that of strain ZY 1–5. Under optimal conditions, the SAM titer and biomass of mutant T11-1 in 5 L bioreactor reached 10.72 g/L and 105.9 g dcw/L (142.86% and 34.22% higher than those of strain ZY 1–5), respectively. Comparative transcriptome analysis between strain ZY 1–5 and mutant T11-1 revealed the enhancements in TCA cycle and gluconeogenesis/glycolysis pathways as well as the inhibitions in serine and ergosterol synthesis of mutant T11-1. The elevated SAM synthesis of mutant T11-1 may attribute to the above changes. Taken together, this study is helpful for industrial production of SAM.
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BIOCHEMICAL ENGINEERING JOURNAL
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2022
·
IF 4.446
Shuping Zou,Zhentao Jiang,Heng Tang,Feng Cheng,Yaping Xue,Yuguo Zheng
The enzymatic asymmetric synthesis of L -phosphinothricin ( L -PPT) from 2-carboxyl-4-(hydroxymethylphosporyl) butyric acid (PPO) is a green and efficient method. In our previous works, we constructed a glutamate dehydrogenase (GluDH) and glucose dehydrogenase (GDH) co-expression strain, Escherichia coli BL21 (DE3)/pETDuet- gludh-gdh , which can asymmetrically reduce PPO to L -PPT. To improve the stability and recyclability of the biocatalyst , we developed a novel method for whole-cell immobilization with cross-linked cell aggregates (CLCAs) and employed immobilized cells in L -PPT production. Montmorillonite was used as a carrier, and dextran polyaldehyde (DPA) was developed as a new cross-linker for cell immobilization . The highest activity recovery (79.2%) was obtained under the following conditions: 20 g/L cell concentration, 0.9% montmorillonite concentration, 1.2% polyethyleneimine (PEI) concentration, and 1.2% DPA concentration. The half-lives of CLCAs were 169.10 h (35 °C), 17.39 h (45 °C), and 10.65 h (55 °C), which were 1.87-, 3.70-, and 10.78-fold than that of free cells, respectively. The CLCAs showed good reusability, retaining over 85% of their initial activities after 10 cycles of utilization. The total yield of L -PPT reached 667 mmol/g cells, and the enantiomeric excess value was more than 99%. The immobilized whole-cell in the form of CLCAs is a valuable biocatalyst for industrial applications.
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SENSORS
·
2022
·Vol. 22
·期 6
·2222
·
IF 3.847
Mengxue Li,Yiwen Kuang,Ziyan Fan,Xiaoli Qin,Shiyu Hu,Zhanning Liang,Qilin Liu,Weizhong Zhang,Birui Wang,Zhaohong Su
Sensitive simultaneous electrochemical sensing of phytohormones indole-3-acetic acid and salicylic acid based on a novel poly(L-Proline) nanoparticles–carbon dots composite consisting of multiwalled carbon nanotubes was reported in this study. The poly(L-Proline) nanoparticles–carbon dots composite was facilely prepared by the hydrothermal method, and L-Proline was used as a monomer and carbon source for the preparation of poly(L-Proline) nanoparticles and carbon dots, respectively. Then, the poly(L-Proline) nanoparticles–carbon dots–multiwalled carbon nanotubes composite was prepared by ultrasonic mixing of poly(L-Proline) nanoparticles–carbon dots composite dispersion and multiwalled carbon nanotubes. Scanning electron microscope, transmission electron microscope, Fourier transform infrared spectroscopy, ultraviolet visible spectroscopy, energy dispersive spectroscopy, cyclic voltammetry, electrochemical impedance spectroscopy, and linear sweep voltammetry were used to characterize the properties of the composite. poly(L-Proline) nanoparticles were found to significantly enhance the conductivity and sensing performance of the composite. Under optimal conditions, the composite-modified electrode exhibited a wide linear range from 0.05 to 25 μM for indole-3-acetic acid and from 0.2 to 60 μM for salicylic acid with detection limits of 0.007 μM and 0.1 μM (S/N= 3), respectively. In addition, the proposed sensor was also applied to simultaneously test indole-3-acetic acid and salicylic acid in real leaf samples with satisfactory recovery.Keywords:poly(L-Proline) nanoparticles;carbon dots;multiwalled carbon nanotubes;indole-3-acetic acid;salicylic acid;simultaneous detection
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Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Shang Xian-chao,Zhang Yu-qin,Zheng Yan-fen,Li Yiqiang
Polyphenols and flavonoids are abundant in spent onion skins (SOSs), being present in amounts are 2–sixfold greater times of than those in the inner bulb. In this study, an eco-friendly and efficient extraction method based on temperature-responsive deep eutectic solvents (TRDESs) was used to obtain natural flavonoids from SOSs. Microwave-assisted extraction yields of quercetin, kaempferol, luteolin, and quercetin-3-O-β-D-glucoside were evaluated to determine the efficiencies of a series of TRDESs. An extraction system composed of ethanolamine and m -cresol at ratio of 1:1 (20% water in TRDES system) was found to possess the highest efficiency. The extraction conditions were screened and optimized for efficiency using a Box-Behnken design. As a result, an extraction power of 554 W, extraction temperature of 76 °C, extraction time of 16 min, and solid–liquid ratio of 14 mg/mL were determined to be the optimal conditions. Under these extraction conditions, the total flavonoid extraction yields reached 47.83 ± 0.21 mg/g. The macroporous resin HPD 600 was selected for the separation and recovery of the TRDESs extraction mixture and the experimental measurements revealed that it allowed recovery rate more than 90%. In vitro activity testing of the flavonoid extract demonstrated excellent FRAP assay, DPPH assay and ABTS assay results for the SOSs extract prepared using a TRDES, and its inhibitory effects on S. aureus , E. carotovora , and B. subtilis were marked.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Hang Xu,Mengfei Zheng,Chenguang Yang,Kun Wang,Zheng Lv,Zhilin Liu,Zhaohui Tang,Xuesi Chen
Glutamine antagonists, such as 6-diazo-5-oxo-L-norleucine (DON), have demonstrated remarkable anti-tumor effects by blocking tumor glutamine metabolism, but their use is frequently causing toxicity. Despite the existence of multiple glutamine antagonist prodrug designs, a tumor-selective prodrug has yet to be developed. Herein, a novel prodrug of DON, Azo-DON, has been developed, which remains stable and inactive in normal tissues with sufficient oxygen levels, while can be selectively reduced to DON by highly expressed azo-reductase in hypoxic tumor environments. This leads to blockade of glutamine metabolism in cancer cells and promotes cell death without affecting T cell proliferation. In a high-hypoxic H22 hepatoma cancer model, Azo-DON showed a 1.8-fold enhancement in glutamine blockade compared to the control group, resulting in a tumor suppression rate (TSR) of 84.2% in vivo with no significant weight loss. In a low-hypoxic CT26 colon cancer model, when combined with vascular disrupting agent nanoparticles (CBP) to induce a hypoxic environment, Azo-DON exhibited a 4.6-fold enhancement in glutamine blockade over the control group, resulted in a remarkable TSR of 96.6% in vivo . This innovative approach represents a promising strategy for the application of broad-spectrum metabolic inhibitors in the field of precision cancer treatment.
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