Frontiers in Plant Science
·
2020
·
IF 4.402
Ji Chao,Liu Zhaoyang,Hao Liping,Song Xin,Wang Changdong,Liu Yue,Li Huying,Li Chaohui,Gao Qixiong,Liu Xunli
The present study investigated the physiological and biochemical characteristics of Enterobacter cloacae HG-1 isolated from saline-alkali soil. We further studied the effect of this strain on the salt tolerance of wheat and on the community structure of nitrogen-fixing bacteria in rhizosphere soil. We determined that the investigated strain had high nitrogen fixation activity and produced iron carriers, 1-aminocyclopropane-1-carboxylic acid deaminase, and plant hormones. The metabolites of this strain contained 2,3-butanediol, [R-(R*, R*)], 2-heptanone, and other growth-promoting and antibacterial substances. The strain was also highly salt-tolerant (10% NaCl). After the inoculation of wheat with the HG-1 strain, we recorded increases in root length, plant height, fresh weight, and dry weight of 19.15%, 18.83%, 16.67%, and 17.96%, respectively, compared with uninoculated plants (P < 0.05). Compared with the leaves of uninoculated plants, the proline concentration in the leaves of inoculated plants increased by 12.43% (P < 0.05), the malondialdehyde level decreased by 27.26% (P < 0.05), K+ increased by 20.69%, Ca2+ increased by 57.53% and Na+ decreased by 31.43% (all P<0.05). Furthermore, we detected that inoculation with the HG-1 strain did not affect the species composition of nitrogen-fixing bacteria in wheat rhizosphere soil at the phylum level. However, the average relative abundance of Proteobacteria was significantly increased, whereas the abundance of Verrucomiorobia was significantly decreased compared with uninoculated plants. At the genus level, we detected 32 genera in control samples and 27 genera in inoculated samples, and the species diversity and relative abundance of samples inoculated with the HG-1 strain decreased compared with uninoculated plants. Inoculated samples had lower abundances of Azospirillum, Rhodomicrobium, and Anabaena. Our study demonstrated that the inoculation of wheat with E. cloacae HG-1 could promote the growth of wheat under salt stress and increase salt stress tolerance. The results of this study investigating the interaction among soil, plants, and microorganisms supplement agricultural microbial databases and could provide a reference for the development of microbial-based saline soil improvement programs.
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PLoS One
·
2019
·Vol. 14
·期 2
·e0212335
·
IF 2.776
Yifan Zhang,Yong Chen,Yiting Cai,Zongyan Cui,Jinjie Zhang,Xiaohou Wang,Lirong Shen
Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal. The cut-off value (sensitivity) of GSIS in detecting MRJP1 is 2.0 μg/mL in solution. Validation analysis with RJ, milk vetch honey, acacia honey and honey adulteration containing rice syrup and corn syrup with different ratios demonstrated that the GSIS could show consistent Test line (T line) when the test samples contain more than 30% pure honey or MRJP1 0.4 mg/g. The validation results by isotope ratio mass spectrometry on the same pure and all adulteration milk vetch honey samples showed the same information of GSIS test. The qualitative assay GSIS provided a valuable new way for honey authenticity and laid the foundation for the future application of GSIS with monoclonal antibodies in honey authentication.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 15
·4712
·
IF 4.927
Yu Lei,Bin He,Shujun Huang,Xinyan Chen,Jian Sun
Facile construction of functional nanomaterials with laccase-like activity is important in sustainable chemistry since laccase is featured as an efficient and promising catalyst especially for phenolic degradation but still has the challenges of high cost, low activity, poor stability and unsatisfied recyclability. In this paper, we report a simple method to synthesize nanozymes with enhanced laccase-like activity by the self-assembly of copper ions with various imidazole derivatives. In the case of 1-methylimidazole as the ligand, the as-synthesized nanozyme (denoted as Cu-MIM) has the highest yield and best activity among the nanozymes prepared. Compared to laccase, theKmof Cu-MIM nanozyme to phenol is much lower, and thevmaxis 6.8 times higher. In addition, Cu-MIM maintains excellent stability in a variety of harsh environments, such as high pH, high temperature, high salt concentration, organic solvents and long-term storage. Based on the Cu-MIM nanozyme, we established a method for quantitatively detecting phenol concentration through a smartphone, which is believed to have important applications in environmental protection, pollutant detection and other fields.Keywords:laccase;phenolic oxidation;nanozyme;intelligent detection;self-assembly synthesis
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Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 6
·772-783
·
IF 4.5
Gao Xiu,Luo Beining,Hong Yanping,He Peihang,Zhang Zedong,Wu Guoqiang
Development of a titanium silicalite-1 (TS-1) catalyst with good crystallinity and a four-coordinate Ti framework is critical for efficient catalytic oxidation reaction under mild conditions. Herein, a size-controlled TS-1 zeolite (TS-1#0.1ACh (acetylcholine)) was synthesized via steam-assisted crystallization by introducing acetylcholine as a crystal growth modifier in the preparation process, and TS-1#0.1ACh was also employed in epoxidations of different substrates containing C=C double bonds. The crystalline sizes of the as-synthesized TS-1#0.1ACh catalysts were controlled with the acetylcholine content, and characterization results showed that the particle sizes of highly crystalline TS-1#0.1ACh zeolite reached 3.0 µm with a good Ti framework. Throughout the synthetic process, the growth rate of the crystals was accelerated by electrostatic interactions between the connected hydroxyl groups of the acetylcholine modifier and the negatively charged skeleton of the pre-zeolites. Furthermore, the TS-1#0.1ACh catalyst demonstrated maximum catalytic activity, good selectivity and high stability during epoxidation of allyl chloride. Importantly, the TS-1#0.1ACh catalyst was also highly versatile and effective with different unsaturated substrates. These findings may provide novel, easily separable and large TS-1 catalysts for efficient and clean industrial epoxidations of C=C double bonds.
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2014
·
IF 3.679
Sheng-Jie Chen,Yong-Qi Li,Yong-Yong Wang,Xiao-Li Zhao,Ye Liu
The ionic hybrid P,N-ligands of 1 and 2 have been synthesized as one type of unconventional tertiary phosphines with the advantages of both the π-accepting ligands and the hemilabile ligands. The corresponding ionic Rh(I)-complexes of 1A and 2A proved to be more efficient catalysts for hydroformylation of 1-octene over isomerization than the traditional Rh I (acac)(CO)(PPh 3 ), due to the π-backdonation interaction in Rh → P linkage and the hemilabile ligation of N,P-coordinating sites to Rh-center. The in situ high-pressure FT-IR analysis indicated that: (1) the formation and lifetime of the active Rh H species (2047 cm −1 ) derived from 1A are facilitated by the presence of ligand 1 ; (2) the formation of the active Rh H species is depressed without the aid of the P,N-hemilabile ligation; and (3) the active Rh H species derived from Rh I (acac)(CO)(PPh 3 ) is unstable and susceptible to the formation of the inactive carbonyl-bridged Rh-dimer.
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JOURNAL OF NEUROTRAUMA
·
2023
·
IF 4.2
Hongxing Tang,Kejun He,Kun Zhao,Chen Zheng,Weichi Wu,Weilin Jin,Lixuan Yang,Baoshu Xie
Hinokitiol (β-thujaplicin, HIK) is a natural monoterpene small molecule compound showing various biological effects including antibacterial, anti- inflammatory and anti-tumor. Ferroptosis, a new form of iron-dependent cell death, has been demonstrated as one of the pathophysiological mechanisms of traumatic brain injury (TBI). However, the effect of hinokitiol on neuronal ferroptosis after traumatic brain injury has not been reported. Here, we show that hinokitiol alleviated excessive glutamate-induced intracellular reactive oxygen species (ROS), lipid peroxidation, and Fe2+ accumulation in HT-22. Moreover, hinokitiol upregulated heme oxygenase-1 (HO-1) expression, promoted nuclear factor-erythroid factor 2-related factor 2 (Nrf2) nuclear translocation, and inhibited the activation of microglia and astrocyte after TBI. Furthermore, hinokitiol remarkably reduced brain tissue lesions, mitigated neuron loss, iron deposition and attenuated neurologic impairment in the CCI-constructed TBI model in vivo. These results suggest that hinokitiol has neuroprotective effects on rescuing cells from TBI-induced neuronal ferroptosis. In summary, we reveal a novel role of hinokitiol in TBI, which has neuroprotective effects on rescuing cells from TBI-induced neuronal ferroptosis. Moreover, hinokitiol through activating the Nrf2/Keap1/HO-1 signaling pathway significantly reduces the neuronal damage, suggesting that it is a potential therapeutic candidate for TBI.
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JOURNAL OF COORDINATION CHEMISTRY
·
2018
·
IF 1.703
Kuan-Guan Liu,Ru Wang,Hong-Yan Han,Qi-De Shan,Jing Nie,Xiao-Wei Yan
Incorporating ferrocenecarboxylato(-1) into [CuI
2(μ-dppm)2]2+ (dppm = bis(diphenylphosphino)methane) led to the isolation of a heterometallic complex [Cu2(μ2-η1,η1dppm)2(μ2-η1, η1FcCOO)(CH3CN)]BF4·CH3CN (1). It was characterized by infrared spectroscopy (IR), elemental analysis (EA), powder X-ray diffraction analysis (PXRD), ultraviolet–visible spectrum (UV–Vis), nuclear magnetic resonance (NMR), and thermogravimetric analysis (TGA). Single-crystal X-ray structural analysis revealed that ferrocenecarboxylato(-1) coordinated to the [Cu2(μ2-η1,η1dppm)2]2+ unit via μ2-η1,η1 bridging mode in 1. The title complex shows good optical absorption and thermal stability, and reliable electrochemical stability. Moreover, this heterometallic copper(I) complex could be used as a promising electrochemical sensing material for the detection of H2O2.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Wenbo Li,Yilin Wang,Dongchen Li,Jiachi Jiang,Kunlan Li,Kaili Zhang,Qingda An,Shangru Zhai,Ligang Wei
The oxidative depolymerization of alkali lignin (AL) in 1-ethyl-3-methylimidazolium acetate ([C 2 C 1 im]OAc) system without additional catalyst was investigated under mild conditions (initial O 2 pressure of 1.5 MPa, 80 °C–100 °C). Compared with other ionic liquids (ILs), the cooperation of imidazolium cation and acetate anion successfully enhanced AL conversion. Among the investigated imidazolium acetate ILs with ethyl- to octyl-side chains, [C 2 C 1 im]OAc presented the best catalytic capacity for AL oxidative depolymerization. Adding an appropriate amount of water to [C 2 C 1 im]OAc can further improve the reaction efficiency. In the [C 2 C 1 im]OAc system with the addition of 0.10–0.25 mL of water, approximately 77 wt% AL was depolymerized into small molecule soluble products at 100 °C for 2 h. The extracted oil was composed mainly of phenolic derived compounds. With the use of the [C 2 C 1 im]OAc-based system, the specific inter-unit linkages of lignin were broken down, and residual lignin with low molecular weight and narrow polydispersity index (1.88–1.96) was obtained. Compared with that in AL conversion with fresh [C 2 C 1 im]OAc, only a minimal decrease (~3.2%) was observed with the recovered IL until the fifth cycle. These findings revealed that [C 2 C 1 im]OAc–based system is a simple and efficient catalytic system for lignin oxidative depolymerization.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2023
·
IF 6.5
Qingwei Jia,Yuqing Yin,Shuting Gai,Lu Tian,Zhihao Zhu,Lei Qin,Yong Wang
Cryptochromes (CRYs), as blue-light photoreceptors, play a crucial role in regulating flowering time and hypocotyl and cotyledon development. Their physiological functions have been extensively studied in various plant species. However, research on onions remains limited. In this study, we identified AcCRY1 and conducted preliminary investigations into its function. Our results demonstrate that AcCRY1 possesses a conserved domain typical of cryptochromes with high homology to those found in monocots. Furthermore, we examined the expression level of AcCRY1 in onion. The green tissues is significantly higher compared to non-green tissues, and it exhibits a significant response to blue-light induction. AcCRY1 demonstrates cytoplasmic localization under blue-light conditions, while it localizes in the nucleus during darkness, indicating a strong dependence on blue-light for its subcellular distribution. In comparison to cry1 , overexpression of AcCRY1 leads to a significant shorten in seedling hypocotyl length, notable expansion of cotyledons, and acceleration of flowering time. The yeast two-hybrid experiment demonstrated the in vitro interaction between AcCRY1, AcCOP1, and AcSPA1. Additionally, BIFC analysis confirmed their interaction in Onion epidermis. Notably, under blue-light conditions, a significantly enhanced binding activity was observed compared to dark conditions. These findings establish a functional foundation for the regulatory role of AcCRY1 in important physiological processes of onion and provide initial insights into the underlying molecular mechanisms.
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JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 18
·12837-12849
·
IF 2.993
Han Zongzhuang,Shen Yu,Wang Fumin,Zhang Xubin
Hierarchical titanium silicalite-1 (TS-1) without generated anatase TiO 2 species was synthesized in the presence of polyquaternium-7 (M550) by one-step hydrothermal crystallization method and characterized by field-emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray fluorescence (XRF), Fourier transform infrared spectra, ultraviolet visible spectra, Raman spectroscopy and N 2 adsorption–desorption measurements. The experimental results show that highly porous TS-1 crystals, with pores of ca. 2.6–4.9 nm diameter, are obtained under hydrothermal conditions by using polyquaternium-7 as the mesopore-directing agent. The polyquaternium-7 has a great influence on both the textural properties and the coordination of Ti species in the framework of TS-1. The catalytic properties and recyclability of the prepared mesoporous TS-1 for hydroxylation of phenol with H 2 O 2 have been evaluated, and their activity has been compared with those of TS-1 with only micropores. The great catalytic activity, selectivity and stability of the hierarchical TS-1 as well as the cheap price and environmental-friendly nature of the used mesopore-directing agent allow to consider this hierarchical TS-1 as a promising catalyst for industry application.
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3 Biotech
·
2019
·Vol. 9
·期 7
·1-9
·
IF 1.786
Yan Hong De,Guo Bing Han,Wang Zhao,Qian Jun Qing
The lipase from Aspergillus oryzae was modified with a surfactant and then observed to exhibit high catalytic efficiency and enantioselectivity for the kinetic resolution of ( RS )-1-phenylethanol. The influential factors of the modified-lipase preparation were investigated, including the surfactant source, the organic cosolvent, and the buffer pH. The optimum modification conditions were found with a surfactant of polyoxyethylene sorbitan monopalmitate, an organic cosolvent of tetrahydrofuran and a phosphate buffer of pH 7.0. In the transesterification of ( RS )-1-phenylethanol with vinyl acetate, the surfactant-modified lipase showed excellent enantioselectivity for the R -isomer ( E > 200), giving an enantiomeric excess of higher than 99% for ( R )-1-phenylethyl acetate at 46.8% conversion with the reaction time of 2 h at 30 °C. The enzymatic activity had barely altered after 30 days even at 50 °C when it was saved in a powdered state. The results indicated that the modification strategy was useful and highly efficient, and that modified A. oryzae lipase was a promising biocatalyst in the kinetic resolution of ( RS )-1-phenylethanol.
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Journal of Physical Chemistry C
·
2020
·
IF 4.189
Yeming Xian,Yongli Zhang,Naveed Ur Rahman,Hang Yin,Yi Long,Peng Liu,Wenzhe Li,Jiandong Fan
Owing to the high toxicity of Pb-based hybrid perovskites, a hypotoxic element, e.g., Sn, was employed to partially or completely replace Pb with the aim of gaining environmentally friendly lead-free photoelectronic device. Herein, for the first time we hydrothermally grow a series of all-inorganic CsSnxPb1–xBr3 (0 ≤ x ≤ 1) perovskite single crystals and carefully study their crystallographic structure and electronic structure that are subject to the varied x value. The phase transition and narrowed band gap from CsPbBr3 to CsSnBr3 are observed, which is similar to the evolution of MASnxPb1–xBr3 perovskites while varying the ratio of Sn/Pb. Likewise, the incremental concentration of Sn atoms in the lattice tend to cause the energy level splitting that likely derives from the orbital overlapping between Sn and/or Pb atoms, which thereby gives rise to the narrowed band gap. Equally important is that the incorporation of Sn atoms is demonstrated to be capable of tightening the electron orbital coupling between Sn and/or Pb atoms, which thereafter enables advantageous balancing of the orbital overlap in the crystal structure with balanced and highly ordered arrangement of [SnBr6]4– and/or [PbBr6]4– octahedra and thus allows us to improve carrier transport and to benefit the real ecofriendly application of photoelectronic devices.
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