MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-10
·
IF 5.7
Liu Yuyao,Deng Yuan,Liao Dongyun,Han Yujie,Wang Qiang,Guo Yujing,Bai Hongcun,Dong Chuan,Fan Lifang
A label-free and efficient electrochemical (EC) sensing platform for di-2-ethylhexyl phthalate (DEHP) was developed based on in situ probe nickel hexacyanoferrate nanoparticle (NiHCF NP)-decorated three-dimensional reduced graphene oxide (3D rGO) composites. NiHCF NPs in the composites as an in situ probe show a pair of well-defined peaks with good reversibility and stability. Coupling 3D rGO with NiHCF NPs not only improved the electron transfer capability of NiHCF NPs but also provided more sites for aptamer immobilization. The synthesized NiHCF NP-decorated 3D rGO composites were used to act as a substrate for the immobilization of anti-DEHP aptamer by the covalent bonding method. The designed EC sensing platform displays excellent sensing performance for DEHP with a low detection limit of 3.64 pg/L, and a linear working range of 0.01 − 1000 ng/L. The application of the sensing platform to actual environmental samples was studied and satisfactory results were obtained. Thus, the proposed EC sensing platform would provide a potential tool for efficient detection of pollutants in the environment. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
·
2023
·
IF 7.4
Qihuang Huo,Yahui Wang,Huijun Chen,Yu Feng,Lina Han,Weiren Bao,Liping Chang,Jiancheng Wang,Kechang Xie
It is a win–win strategy to prepare mercury sorbents from low-cost high-sulfur raw materials. In part 1, mercury sorbents with excellent performance were prepared from high inorganic sulfur coal through KOH activation. However, this method does not work for high organic sulfur coal. In this work, a two-step preparation method is developed to prepare mercury sorbents from high organic sulfur coal. The first step is the co-pyrolysis/carbonization of Fe 2 O 3 and coal, during which organic sulfur in coal is converted into FeS x . XPS shows that the percentage of S x 2– /S on the surface of the carbonized samples gradually increases from 5.4% to 22.2% with the m(Fe):m(S o ) ratio from 0:4 to 5:4. The second step is KOH activation, and it converts FeS x into various S species, including C-S, S 0 , S 2 2− and S 2− . Among the prepared mercury sorbents, LF+3Fe/4S-A (m(Fe):m(S o ) = 3:4) shows the best mercury removal performance, and its mercury removal efficiency remains above 84% for 120 min under N 2 +O 2 at 120 °C. The Hg-TPDD and XPS results indicate that S*, including C-S, S 0 and S 2 2− , are the major active sites for mercury removal rather than Fe element, and HgS is the main existing form of mercury on the used sorbents. Besides LF coal, this two-step process is also applicable to other high organic sulfur coals (such as LL coal, WJZ coal, and SY coal) and high-sulfur petroleum coke.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 7
·3628-3637
·
IF 4.1
Yuanyuan Xue,Youwu Liao,Haiqiao Wang,Shuang Li,Zhengqing Gu,Michael Adu-Frimpong,Jiangnan Yu,Ximing Xu,Hugh D. C Smyth,Yuan Zhu
BACKGROUND Astaxanthin is a type of food-derived active ingredient with antioxidant, antidiabetic and non-toxicity functions, but its poor solubility and low bioavailability hinder further application in food industry. In the present study, through inclusion technologies, micellar solubilization and electrospray techniques, we prepared astaxanthin nanoparticles before optimizing the formulation to regulate the physical and chemical properties of micelles. We accomplished the preparation of astaxanthin nanoparticle delivery system based on single needle electrospray technology through use of 2-hydroxypropyl-β-cyclodextrin and Soluplus® to improveme the release behavior of the nanocarrier. RESULTS Through this experiment, we successfully prepared astaxanthin nanoparticles with a particle size of approximately 80 nm, which was further verified with scanning electron microscopy and transmission electron microscopy. Furthermore, the encapsulation of astaxanthin molecules into the carrier nanoparticles was verified via the results of attenuated total reflectance intensity and X-ray powder diffraction techniques. The in vitro release behavior of astaxanthin nanoparticles was different in media that contained 0.5% Tween 80 (pH 1.2, 4.5 and 6.8) buffer solution and distilled water. Also, we carried out a pharmacokinetic study of astaxanthin nanoparticles, in which it was observed that astaxanthin nanoparticle showed an effect of immediate release and significant improved bioavailability. CONCLUSION 2-hydroxypropyl-β-cyclodextrin and Soluplus® were used in the present study as a hydrophilic nanocarrier that could provide a simple way of encapsulating natural function food with repsect to improving the solubility and bioavailability of poorly water-soluble ingredients. © 2023 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
·
2023
·
IF 3.5
Hao CHEN,Zhi Yuan HOU,Die CHEN,Ting LI,Yi Ming WANG,Marcelo Andrade DE LIMA,Ying YANG,Zhen Zhong GUO
Objective Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2. Methods In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry. Results Under optimal experimental conditions, the linear range of the sensor was 10 −13 –10 −9 mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability. Conclusion The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Tao Peng,Lianhua Dong,Xiaoli Feng,Yi Yang,Xia Wang,Chunyan Niu,Zhanwei Liang,Wang Qu,Qingcui Zou,Xinhua Dai,Minghua Li,Xiang Fang
More than forty antigen testing kits have been approved to response the prevalence of SARS-CoV-2 and its variant strains. However, the approved antigen testing kits are not capable of quantitative detection. Here, we successfully developed a lateral flow immunoassay based on colloidal gold nanoparticles (CGNP-based LFIA) for nucleocapsid (N) protein of SARS-CoV-2 quantitative detection. Delta strain (NMDC60042793) of SARS-CoV-2 have been cultured and analyzed by our developed digital PCR and LFIA methods to explore the relationship between N protein amount and N gene level. It indicated that the linear relationship (y = 47 ×) between N protein molecule number and N gene copy number exhibited very well (R 2 = 0.995), the virus titers and N protein amount can be roughly estimated according to nucleic acid testing. Additionally, detection limits (LODs) of nine approved antigen testing kits also have been evaluated according to the Guidelines for the registration review of 2019-nCoV antigen testing reagents. Only three antigen testing kits had LODs as stated in the instructions, the LODs of Kits have been converted into the N gene and N protein levels, according to the established relationships among virus titer vers. N gene and antigen. Results demonstrated that the sensitivity of nucleic acid testing is at least 1835 times higher than that of antigen testing. We expect that the relationship investigation and testing kits evaluation have the important directive significance to precise epidemic prevention.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Wei-Ran Han,Wen-Long Wang,Tie-Jun Qiao,Wei Wang,Hang Su,Chen-Xin Xu,Qian-Yuan Wu
Micro-bubble aeration is an efficient way to promote ozonation performance, but the technology is challenged by extensive energy cost. Here, a ceramic ultrafiltration membrane was used to achieve ozone micro-bubble (0–80 µm) aeration in a simple way at gaseous pressures of 0.14–0.19 MPa. Compared with milli-bubble aeration, micro-bubble aeration increased the equilibrium aquatic O 3 concentrations by 1.53–3.25 times and apparent O 3 transfer rates by 3.12–3.35 times at pH 5.0–8.0. Consequently, the • OH yield was 2.67–3.54 times via faster O 3 transfer to the aquatic solution followed by decomposition rather than interfacial reaction. Ozone micro-bubble aeration outperformed milli-bubble aeration, with the degradation kinetics of 2,4-D being 3.08–4.36 times higher. Both O 3 -oxidation and • OH oxidation were important to the promotion with the contributions being 35.8%-45.9% and 54.1%-64.2%, respectively. The operational and water matric conditions influenced the oxidation performance via both O 3 oxidation and • OH oxidation, which is reported for the first time. In general, the ceramic membrane offered a low-energy approach of ozone micro-bubble aeration for efficient pollutant degradation. The O 3 oxidation and • OH oxidation were proportionally promoted by ozone micro-bubble due to O 3 transfer enhancement. Thus, the promotive mechanism can be interpreted as the synchronous enchantment on ozone exposure and • OH exposure for the first time.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2023
·
IF 5.4
Qiumin Peng,Wanting Huang,Duo Chen,Zhipeng Gao,Yanbing Yang,Quan Yuan
Comprehensive Summary With the advantages of high sensitivity, rapid response, label-free, and simple operation, field effect transistor biosensors have shown promising application prospects in large-scale pathogen screening. However, in practical biological fluids with relatively high ionic strength, such as saliva and serum, the Debye screening effect will weaken the interaction between FET biosensors and target bio-molecules, thereby affecting the sensing sensitivity and accuracy. Herein, an interfacial polymer-engineered field effect transistor (IPE FET) biosensor was developed for the efficient identification of SARS-CoV-2 N antigens in saliva samples. The inclusion of a polymer layer shortens the distance between target molecules and the electrode interface, which effectively overcomes the limitation of Debye screening. The constructed IPE FET biosensors exhibit high sensitivity and anti-fouling capability, achieving efficient detection of SARS-CoV-2 N antigen in saliva within 5 min with a detection limit of 4.6 fg·mL −1 . In a cohort of 35 simulated throat swab samples of SARS-CoV-2 N antigen, IPE FET exhibits an identification accuracy of up to 97.1%, with predictive sensitivity and specificity of 96.0% and 100.0%, respectively. The excellent performance of IPE FET not only provides a strategy to design efficient detection platforms but also suggests a pathway to realize rapid and scalable epidemic screening.
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2023
·
IF 4.3
Liqun Huang,Ge Sun,Wenli Xu,Shufang Li,Xiujun Qin,Quan An,Zhongwen Wang,Jianguo Li
The objective of this study was to explore the endocytosis mechanisms of uranium uptake in HK-2 cells and its toxic effects. Our results demonstrated that uranium exposure impairs redox homeostasis and increases the permeability of the cell membrane and mitochondrial membrane, which may induce cell apoptosis by cytochrome-c leakage . Alkaline phosphatase activity increased after uranium exposure, which may be involved in the process of intracellular mineralisation of uranium, leading to severe cell necrosis. Furthermore, our findings demonstrated that the clathrin-mediated endocytosis process contributed substantially to uranium uptake in HK-2 cells and the total uranium uptake was highly correlated with cell viability, reaching a high correlation coefficient (r = −0.853) according to Pearson correlation analysis. In conclusion, the uptake of uranium into mammalian cells was mainly facilitated by the clathrin-mediated endocytosis pathway and induced dose-dependent cellular toxicity, including redox homeostasis imbalance, membrane injury, cell apoptosis and necrosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2022
·
IF 7.139
Zuqin Duan,Qian Lei,Wenhui Wu,Honglin Chen
Hydrogen release from formic acid is a significant energy supply route. However, the current catalysts suffer from low catalytic efficiency and stability. Herein, a porous N-doped carbon material with high N content (16.87%) and a large surface area (1544 m 2 ·g −1 ) were designed using a 2-dimensional metal-organic framework and etching agent potassium chloride . Due to its high N content and large surface area, ultrafine Pd particles are uniformly distributed on the porous N-doped carbon support, which effectively enhances excellent reactivity and enables a TOF value of 2365 h −1 under additive-free conditions. The research also revealed that the strong interaction between Pd particles and pyridinic-N species can significantly slow metal agglomeration. Hence, the Pd/NC ZIF-L (KCl) displayed good activity even after 10 cycling experiments, and it is a particularly competitive catalyst for hydrogen release from formic acid.
✓ 已验证
DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Niu Ting,Wei Zhiying,Fu Jiao,Chen Shu,Wang Ru,Wang Yuya,Zheng Ruihe
Introduction: Venlafaxine is one of the most commonly used anti-depressant and antineoplastic drug. Previous studies have predicted venlafaxine as an anti-cancer compound, but the therapeutic effects of venlafaxine in melanoma have not yet been demonstrated. Nur77 is an orphan nuclear receptor that highly expressed in melanoma cells and can interact with Bcl-2 to convert Bcl-2 from an antiapoptotic to a pro-apoptotic protein.Method: We examined the effects of venlafaxine in MV3 cells in vitro and MV3 xenograft tumor in nude mice. Western-blot, PCR, TUNEL assay and immunofluorescence were used to reveal the growth of melanoma cells.Results: Here, our data revealed that venlafaxine could reduce the growth, and induce apoptosis of melanoma cells through a Nur77-dependent way. Our results also showed that treatment with venlafaxine (20 mg/kg, i.p.) potently inhibited the growth of melanoma cells in nude mice. Mechanistically, venlafaxine activated JNK1/2 signaling, induced Nur77 expressions and mitochondrial localization, thereby promoting apoptosis of melanoma cells. Knockdown of Nur77 and JNK1/2, or inhibition of JNK1/2 signaling with its inhibitor SP600125 attenuated the anti-cancer effects of venlafaxine.Conclusion: In summary, our results suggested venlafaxine as a potential therapy for melanoma.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 48
·5091-5099
·
IF 3.532
Yue Sun,Zihao Xie,Fubin Pei,Wei Hu,Shasha Feng,Qingli Hao,Bing Liu,Xihui Mu,Wu Lei,Zhaoyang Tong
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seriously threatened global public health. Establishing a rapid and sensitive diagnostic test for early detection of the SARS-CoV-2 nucleocapsid protein is urgently required to defend against the pandemic. Herein, an enhanced lateral flow immunoassay (LFIA) was fabricated by trimetallic Au@Pd@Pt core–shell nanozymes for detection of the SARS-CoV-2 nucleocapsid protein. The Au@Pd@Pt nanozymes (Au@Pd@Pt NZs) synthesized via a one-pot method, with a dendrite morphology and uniform particle size, showed excellent peroxidase-like activity. Due to the perfect enzyme-like catalytic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), the catalytic signal could be generated even by a tiny amount of Au@Pd@Pt NZs accumulated on the test strip. Therefore, rapid detection with higher sensitivity was achieved. The Au@Pd@Pt NZs-based LFIA provided a quantitative range of 0.05–100 ng mL−1 with a limit of detection of 0.037 ng mL−1, which was 17-fold lower than the LFIA without enhancement. The average recoveries from spiked samples were in the range of 92.5–107.9% with relative standard deviations all less than 4%, indicating the reliability and repeatability of the proposed LFIA. Additionally, the proposed LFIA could report results within 30 min using a microplate reader. In conclusion, the Au@Pd@Pt NZs-LFIA is a rapid, simple, and sensitive method for detecting the SARS-CoV-2 nucleocapsid protein.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Kai Zhang,Meina Yang,Helin Sun,Xueyin Wang,Hao Li,Jinxiang Han,Zhongwen Zhang
Using Peptide nucleic acid (PNA), this work provides a unique approach for controlling CRISPR/Cas13a activity by RNA Inhibitory PNA (RIP). This method reduces the action of CRISPR/Cas13a by inhibiting the binding of crRNA to CRISPR/Cas13a, which was subsequently utilized to detect metalloproteinase-2 (MMP-2) activity. We carefully constructed a PNA sequence that includes a peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) that is particularly cleaved by MMP-2 in the middle. When MMP-2 is not present in the system, the PNA/crRNA complex reduces crRNA binding to CRISPR/Cas13a, hence inhibiting CRISPR/Cas13a cleavage activity. When MMP-2 is present in the system, it cleaves the peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) selectively and so prevents PNA from binding to crRNA, enabling crRNA to reach the "free" form. Then, in the presence of an RNA activator, crRNA may bind to CRISPR/Cas13a and stimulate its activity. CRISPR/Cas13a triggered in this manner may precisely cleave the DNA sequence containing modified -UU- bases on the electrode surface . Thus, the ferrocene (Fc) is changed away from the electrode surface at one end of the DNA , enabling for the recovery of the Electrochemiluminescence (ECL) signal from the two-dimensional material transformed on the electrode surface. As a result, MMP-2 activity was detected utilizing this technique of inhibiting crRNA activity with a detection limit of 15.56 fM. Our suggested method of inhibiting CRISPR/Cas13a activity via PNA has a broad variety of potential therapeutic applications in gene editing and the development of CRISPR/Cas13a-based biosensors.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）