Green Energy & Environment
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2025
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IF 14.6
Runqing Xiao,Qingmao Yang,Yanjie Li,Wei Zhang,Gang Xiao,Chun Shen
Achieving high selectivity to 2,5-furandicarboxylic acid (FDCA) in the photocatalytic oxidation of 5-hydroxymethylfurfural (HMF) in aqueous solution advocates the principle of green and sustainable chemistry, but still remains a significant challenge. Herein, manipulating the reactive oxygen species (ROS) has been realized and dramatically promotes the selective photocatalytic oxidation of HMF in aqueous solution. High FDCA yield of 98.6% has been achieved after 3 hours of visible light irradiation over the as-prepared FeO x -Au/TiO 2 catalyst, being one of the leading photocatalytic performances. Furthermore, satisfactory FDCA yields of higher than 80% could be realized even in the outdoor environment under natural sunlight irradiation, regardless of sunny or cloudy weather. A combination study including physical characterization, kinetic analysis, radical trapping experiments and density functional theory calculations unveils the rate-determining step (oxidation of hydroxyl group) and respective contributions of the generated ROS ( 1 O 2 and •O 2 – ) in each step of the entire reaction network. The present work would push ahead the understanding of HMF photocatalytic oxidation and contribute to the rational design of high-performance photocatalysts.
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PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
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2014
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IF 1.335
Yuming Dong,Zhengjie Zhou,Hongyan Ding,Shujiang Zhang
Context: High concentration of 5-amino salicylic acid (5-ASA) in the distal ileum and colon is necessary for the treatment of inflammatory bowel disease (IBD). The control of small molecules, drugs, released from a polymeric matrix remains a great challenge.Objective: To study the preparation and properties of a pH-sensitive carrier for targeting delivery of 5-ASA.Materials and methods: The carrier was prepared by ternary blends method based on polyvinyl alcohol (PVA), sodium alginate (SA) and polylactic acid. It was characterized by infrared spectrometry and scanning electronic microscopy. The adsorption and release of 5-ASA in different pH media were investigated.Results: We found out the best ratio of the materials for synthetic carrier. The vector exhibited good performance by the controlled release of the target drug experiment. The adsorption capacity of the carrier for 5-ASA was 70.34% in phosphate buffer saline at pH 1.00, and the release rate was 100.49% in phosphate buffer solution at pH 6.80.Discussion and conclusion: PVA is vector backbone of the carrier, and SA plays key role in its pH performance. It is a promising material to effectively deliver 5-ASA to the specific sites of IBD.
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JOURNAL OF THE ENERGY INSTITUTE
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2025
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IF 6.2
Ziwen Zhao,Hong Tian,Zhengye Chen,Zhangjun Huang,Shan Cheng,Siying Liu
This study employed torrefied wheat straw (260°C) as feedstock to synthesise a bifunctional metal-modified composite zeolite catalyst through alkali treatment (NaOH), dual metal loading (Fe and Ni), and hierarchical MCM-41 construction. Catalytically assisted pyrolysis behaviour was investigated using a tube furnace and Py-GC/MS system, evaluating the effects of pyrolysis temperature and catalyst/feedstock mixing ratio on product distribution and bio-oil composition. Results indicate that at a catalyst/feedstock ratio of 3:2, bio-oil yield peaked at 28.41% at 500°C, concurrently achieving a maximum monocyclic aromatic content of 66.36%. Elevating pyrolysis temperature promoted aromatic formation while significantly suppressing oxygenated compounds; notably, phenolic components decreased by 13.94% at 500°C, indicating the 1Ni1FeHR@M catalyst's potent deoxygenation capability. Further optimisation of the catalyst/feedstock blending ratio revealed that a 3:2 ratio yielded the highest combined bio-oil and monocyclic aromatic hydrocarbon production (18.62%), confirming this catalytic strategy effectively shifts the product distribution towards higher-value hydrocarbon fuels.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2019
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IF 3.553
Liuyan Gu,Haibo Yuan,Xueqin Lv,Guangsheng Li,Rigang Cong,Jianghua Li,Guocheng Du,Long Liu
5-Methylpyrazine-2-carboxylic acid (MPCA) is an important pharmaceutical intermediate and is used in the production of hypoglycemic agents and lipid-lowering drugs. This work aimed to develop a whole-cell biocatalytic process for the efficient synthesis of MPCA from 2, 5-dimethylpyrazine (DMP). Firstly, a whole-cell biocatalyst Escherichia coli strain was constructed by plasmid-based expression of xylene monooxygenase (XMO), benzyl alcohol dehydrogenase (BADH), and benzaldehyde dehydrogenase (BZDH) from Pseudomonas putida ATCC 33015, resulting in MPCA titer of 5.0 g/L. Then, the reaction conditions were optimized and the MPCA titer was further increased to 9.1 g/L. Next, the Ribosome Binding Site (RBS) Calculator v2.0 was used to predict and compare the translation initiation rates of the RBS sequences preceding xylM and xylA genes, encoding the two subunits of XMO. By optimizing the RBS sequences preceding xylA , the MPCA titer was increased to 10.2 g/L and the yield of MPCA on DMP reached 0.665 mol/mol. Finally, to achieve plasmid-free production of MPCA, we integrated the genes encoding for XMO, BADH and BZDH in the genome by using CRISPR/Cas9 and further fine-tuned the copy number ratios of xylM and xylA in the genome, improving the MPCA titer to 15.6 g/L and the yield of MPCA on DMP to 1.0 mol/mol. This work developed a high-yield and plasmid-free biocatalysis process for the environmentally friendly production of MPCA with 100% substrate conversion, and paved the way for the commercial production of MPCA in the future.
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JOURNAL OF NUTRITIONAL BIOCHEMISTRY
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2020
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IF 4.873
Liu Tang,Xiao-Lei Li,Zi-Xin Deng,Yao Xiao,Ya-Hong Cheng,Jing Li,Hong Ding
Conjugated linoleic acid (CLA), commonly found in beef, lamb and dairy products, has been reported to exhibit anti-inflammatory and antipruritus effects and to inhibit the release of chemical mediators such as histamine and eicosanoid in laboratory rodents. The chief objective of the study is to assess the efficacy of CLA on atopic dermatitis (AD) in mice and to explore possible mechanisms with CLA treatments . To develop a new therapy for AD, the anti-AD potential of CLA was investigated by inducing AD-like skin lesions in mice using 2,4-dinitrofluorobenzene. We evaluated dermatitis severity; histopathological changes; serum levels of T helper (Th) cytokines (interferon-γ, interleukin-4); changes in protein expression by western blotting and immunohistochemistry staining for cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX), toll like receptor 4 (TLR-4), myeloid differentiation factor 88 (MyD88), nuclear factor-κB (NF-κB) and tumor necrosis factor α (TNF-α); and production of the proinflammatory lipid mediators, such as prostaglandin E 2 and leukotriene B4 , in the skin lesions. Treatment with CLA ameliorated the development of AD-like clinical symptoms and effectively inhibited epidermal hyperplasia and infiltration of mast cells and CD4 + T cells in the AD mouse skin. Total serum immunoglobulin E levels and the expression levels of Th1/Th2 cytokines and lipid mediators in dorsal skin were dramatically suppressed by CLA. Furthermore, CLA down-regulated the expressions of COX-2, 5-LOX, TLR4, MyD88, NF-κB and TNF-α. Taken together, our findings demonstrate the potential usefulness of CLA as an anti-inflammatory dietary supplement or drug for the prevention and management of AD skin diseases by modulating the COX-2/5-LOX and TLR4/MyD88/NF-κB signaling pathways .
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Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 37
·7347-7354
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IF 4.726
Chao Qi,Ying-Jie Zhu,Yong-Gang Zhang,Ying-Ying Jiang,Jin Wu,Feng Chen
A rapid and simple strategy is reported for the synthesis of amorphous calcium phosphate (ACP) vesicle-like nanospheres using the adenosine 5′-triphosphate (ATP) disodium salt as a biocompatible phosphorus source and stabilizer by the sonochemical method in mixed solvents of water and ethylene glycol (EG). The ACP vesicle-like nanospheres are characterized by X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, UV/Vis absorption spectroscopy, thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), dynamic light scattering (DLS) and Brunauer–Emmett–Teller (BET) nitrogen adsorption. The ACP vesicle-like nanospheres exhibit essentially inappreciable toxicity to the cells in vitro. Furthermore, the as-prepared ACP vesicle-like nanospheres can be used as anticancer drug nanocarriers and show a pH-responsive drug release behaviour using doxorubicin (Dox) as a model drug. The ACP vesicle-like nanosphere drug delivery system exhibits a high ability to damage cancer cells, thus, it is promising for application in pH-responsive drug delivery.
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EXPERIMENTAL ANIMALS
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2022
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IF 1.798
Zhanchun SONG,Xiaozeng WANG,Lianqi HE,Liang CHEN,Zhichao REN,Siyu SONG
Myocardial infarction (MI) as the remarkable presentation of coronary artery disease is still a reason for morbidity and mortality in worldwide. Lysosomal-associated protein transmembrane 5 (LAPTM5) is a lysosomal-related protein found in hematopoietic tissues and has been confirmed as a positive regulator of pro-inflammatory pathways in macrophages. However, the role of LAPTM5 in MI remains unknown. In this study, we found that both mRNA and protein expression levels of LAPTM5 were significantly elevated in MI mice. Suppression of LAPTM5 in myocardial tissues decreased cardiac fibrosis and improved cardiac function after MI. At the molecular level, downregulated LAPTM5 dramatically suppressed the macrophage activation and inflammatory response via inhibiting the activation of the NF-κB pathway. Collectively, suppression of LAPTM5 in myocardial tissues inhibits the pro-inflammatory response and the cardiac dysfunction caused by MI. This study indicated that LAPTM5 as a pro-inflammatory factor plays a crucial role in MI disease.
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ANALYTICA CHIMICA ACTA
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2013
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IF 4.387
Debin Wan,Hongmei Yang,Fengrui Song,Zhiqiang Liu,Shuying Liu
1-Phenyl-3-methyl-5-pyrazolone (PMP) labeling technique has hitherto proved to be a convenient and sensitive method for separating and detecting oligosaccharides. However, the detailed fragmentation of the derivatives by tandem mass spectrometry has been reported limitedly and no characteristic fragment ions for isomers have been detected. In this study, eight disaccharide isomers were labeled with PMP and analyzed by positive ion electrospray ionization multi-stage tandem mass spectrometry (ESI-MS n ). In comparison with the native disaccharides, PMP labeled disaccharides gave rise to more fragment ions in the tandem mass spectra. The distinctive diagnostic fragment ions formed from cleavage of C C bonds have been detected in the fragmentation of PMP-labeled disaccharide linkage isomers, allowing unambiguous assignment of the position of the glycosidic linkages. This feature is particularly useful for the structural determination of unknown isomeric disaccharides mixed together. In addition, the anomeric configurations can also be easily assigned based on the relative abundance ratios of the selected ion pairs. To verify the feasibility of the method used in the analysis of natural product, water soluble Panax Ginseng extract has been further investigated to identify its unknown disaccharides. The results confirmed that the PMP labeling technique in conjunction with ESI-MS n could offer a powerful and convenient tool for differentiation of structurally closely related isomers, even the unknown mixtures of isomeric disaccharides with different linkage types.
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Reaction Kinetics Mechanisms and Catalysis
·
2024
·Vol. 137
·期 3
·1635-1649
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IF 1.7
Pan Weixing,Chen Hui,Chen Aicheng
A series of low-cost α-Ni(OH) 2 /SiO 2 catalysts were successfully fabricated by the vapor-induced internal hydrolysis method, then the hydrogenation of 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF) was investigated by using the ethanol as the hydrogen donor over these as-prepared α-Ni(OH) 2 /SiO 2 catalysts. Based on the reaction results, these as-prepared catalysts, which could dispense with extra pre-reduction treatment before reactions, exhibited superior catalytic performance for the HMF hydrogenation to BHMF. A remarkable intrinsic selectivity of BHMF selectivity of almost 100% with about 90% HMF conversion could be obtained under 195 °C for 5 h in an N 2 atmosphere over the α-Ni(OH) 2 /SiO 2 catalyst of 5-Ni(OH) 2 /SiO 2 with about 5 wt% Ni on SiO 2 . The almost constant HMF conversion and BHMF intrinsic selectivity found from the recycling tests can indicate that the catalyst was stable without the obvious loss of its catalytic activity after recycling. Moreover, the mechanism of HMF hydrogenation using ethanol over the α-Ni(OH) 2 /SiO 2 catalysts could be referred to as the mechanism of the Meerwein-Ponndorf-Verley reaction. This work will provide a simple synthesis method for future design and development of high-performance, low-cost, and low-energy consumption catalysts for HMF hydrogenation to BHMF.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Zuhao Chen,Yongyou Hu,Guanglei Qiu,Donghui Liang,Jianhua Cheng,Yuancai Chen,Guobin Wang,Xiaoqiang Zhu,Jieyun Xie
Inoculating functional bacterial strains is a cost-effective strategy for enhancing treatment of anaerobic digestion liquids in swine wastewater. This study systematically evaluated inoculation of heterotrophic nitrification aerobic denitrification strain Acinetobacter indicus CZH-5 in an internal circulation airlift zeolite sphere-based sequencing batch reactor (IR) for aerobic removal of nitrogen (N), phosphorus (P), and tetracycline (TEC). Inoculation with CZH-5 promoted secretion of quorum sensing signaling molecules, specifically N-acyl-homoserine lactones (C6-HSL and C10-HSL). These signaling molecules enhance quorum sensing and reinforce cooperation among functional bacteria. Under optimal conditions, average removal efficiencies of total nitrogen, total phosphate, and TEC were 92.8%, 88.4%, and 93.1%, respectively. The removal performance in IR exceeded that of the control by 26%–71%. N removal involved complete nitrification-denitrification, while accumulated P was transformed into phosphate monoesters within biofilm. Metagenomic analysis identified Thauera and Acinetobacter as the dominant genera, and Acinetobacter indicus as predominant species. Inoculation enhanced microbial richness and diversity to improve system operational stability. The abundance of functional genes associated with N, P, and TEC transformations significantly increased compared to the control. This study aimed to investigate the characteristics and mechanisms of inoculating a heterotrophic nitrification aerobic denitrification strain into an aerated biofilm system for swine wastewater remediation.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Haoheng Wu,Yanyan Yu,Ying He,Yun Liu
The development of high-performance, multifunctional catalysts capable of effectively addressing nitroarene hydrogenation remains a significant challenge. In this study, we present a novel approach to catalyst design through the controlled carbonization of a copper–furan dicarboxylate metal–organic framework (Cu-FDCA MOF), resulting in two distinct nanocomposites: oxygen-vacancy-rich Cu/Cu 2 O/CuO@C (synthesized in an argon atmosphere) and conventional CuO (prepared by air calcination). Advanced characterizations, including TEM, XPS, EPR, and BET, reveal that Cu/Cu 2 O/CuO@C possesses 2.3 times more oxygen vacancies and a surface area 97 times greater (106.21 m 2 /g vs. 1.09 m 2 /g) than CuO, which directly correlates with its superior catalytic performance. In the context of nitroarene hydrogenation, Cu/Cu 2 O/CuO@C demonstrates an exceptionally high rate constant (k app = 25.33 × 10 -2 s -1 ), outperforming CuO by a factor of 40. Furthermore, Cu/Cu 2 O/CuO@C maintains 90 % of its catalytic activity after five reaction cycles and can be fully regenerated upon treatment at 400 °C. Through a combination of experimental studies and density functional theory (DFT) calculations, we propose a novel water-derived hydrogen transfer mechanism that challenges conventional understanding of the role of NaBH 4 . Notably, Cu/Cu 2 O/CuO@C also exhibits impressive versatility, efficiently degrading organic dye (Congo red/methylene blue/methyl blue) within three minutes. This work establishes MOF-derived oxygen-deficient nanocomposites as promising platforms for both amine synthesis and environmental decontamination.
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ACS Nano
·
2025
·
IF 16
Chendong Yang,Entao Li,Xiaoping Guo,Wenyu Xie,Yuanzhan Wang,Xuefei Huang,Sandra Chiu,Xuanjun Wu
Over the past 3 years, SARS-CoV-2 Omicron has been circulating globally with the emergence of multiple subvariants, including BA.5, BA.5.2, XBB, XBB.1, EG.5.1, HK.3, BA.2.86, JN.1, and KP.2. To combat these Omicron subvariants, several vaccines based on receptor-binding domain (RBD) dimers have been developed; however, RBD dimer vaccines require frequent updates to cope with the emergence of new variants. In contrast, the development of a safe, effective, and broad-spectrum vaccine against multiple Omicron subvariants, including the latest JN.1 and KP.2, would be a one-size-fits-all solution. Here, we designed BA.4/5 RBD-PC7A conjugate micelles by displaying the BA.4/5 RBD in PC7A micelles. Remarkably, the micelles elicited potent neutralizing antibodies (NAbs) in rabbits, effectively neutralizing BA.5.2, XBB.1.18, and HK.3 infections. Moreover, the micelles alone were able to induce NAbs in mice against the BA.5 variant. When a cytosine-phosphate-guanine (CpG) adjuvant was added and electrostatically adsorbed to the micelles, there was a significant increase in the antibody titers of IgG1, IgG2b, and IgG2c. This enhancement facilitated the broad neutralization of various strains, including BA.5.2, XBB.1.18, HK.3, JN.1, and KP.2. Furthermore, the micelles adsorbed with CpG protected golden hamsters from infection with the BA.5.2 strain. This study presents a potent and broadly neutralizing nanovaccine that includes the BA.4/5 RBD antigen and a CpG adjuvant. It demonstrates efficacy against multiple Omicron subvariants, including BA.5, BA.5.2, XBB.1.18, HK.3, JN.1, and KP.2, highlighting its potential for clinical translation.
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