Foods
·
2023
·Vol. 12
·期 23
·4364
·
IF 5.2
Huibing Chi,Qingwei Jiang,Yiqian Feng,Guizheng Zhang,Yilian Wang,Ping Zhu,Zhaoxin Lu,Fengxia Lu
Acrylamide is present in thermally processed foods, and it possesses toxic and carcinogenic properties. L-asparaginases could effectively regulate the formation of acrylamide at the source. However, current L-asparaginases have drawbacks such as poor thermal stability, low catalytic activity, and poor substrate specificity, thereby restricting their utility in the food industry. To address this issue, this study employed consensus design to predict the crucial residues influencing the thermal stability ofCorynebacterium glutamicumL-asparaginase (CgASNase). Subsequently, a combination of site-point saturating mutation and combinatorial mutation techniques was applied to generate the double-mutant enzyme L42T/S213N. Remarkably, L42T/S213N displayed significantly enhanced thermal stability without a substantial impact on its enzymatic activity. Notably, its half-life at 40 °C reached an impressive 13.29 ± 0.91 min, surpassing that of CgASNase (3.24 ± 0.23 min). Moreover, the enhanced thermal stability of L42T/S213N can be attributed to an increased positive surface charge and a more symmetrical positive potential, as revealed by three-dimensional structural simulations and structure comparison analyses. To assess the impact of L42T/S213N on acrylamide removal in biscuits, the optimal treatment conditions for acrylamide removal were determined through a combination of one-way and orthogonal tests, with an enzyme dosage of 300 IU/kg flour, an enzyme reaction temperature of 40 °C, and an enzyme reaction time of 30 min. Under these conditions, compared to the control (464.74 ± 6.68 µg/kg), the acrylamide reduction in double-mutant-enzyme-treated biscuits was 85.31%, while the reduction in wild-type-treated biscuits was 68.78%. These results suggest that L42T/S213N is a promising candidate for industrial applications of L-asparaginase.Keywords:Corynebacterium glutamicum;L-asparaginase;thermal stability;consensus design;acrylamide;biscuits
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PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Xin Yin,Muhammad Aslam Khan,Paul Van der Meeren,Shuai Feng,Yongchao Wang,Li Liang
Co-encapsulation of bioactive compounds with different functionality in a carrier has received considerable attention, while co-encapsulation of bioactives with different solubility remains a challenge. In this study, the co-encapsulation of hydrophilic l -ascorbate and hydrophobic curcumin was achieved using an organic-free method. The aim of this work was to evaluate the efficacy of chitosan coating loaded with l -ascorbate on the complexation of curcumin with caseinate by investigating their interaction, storage stability and release behavior during simulated digestion. The size and ζ-potential of the optimized chitosan-caseinate binary particles at pH 5.0 were 582 nm and 28 mV, respectively. l -ascorbate electrostatically interacted with chitosan and then coated on the caseinate particles where hydrophobic curcumin was encapsulated. The encapsulation efficiency of curcumin and l -ascorbate was 96.2% and 47.6%, respectively. l -ascorbate-loaded chitosan coating significantly improved the storage and irradiation stability of curcumin and inhibited the structural changes of caseinate induced by the loaded curcumin after storage. The antioxidant activity of the co-delivered system was significantly improved compared with either individual system and basically unchanged during irradiation. These results may serve as a helpful guide for future study on the optimization of core-shell particles for the co-delivery of multiple bioactive compounds.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Lina Qiao,Jing Wang,Bing Liu,Junyi Wang,Ruifang Wang,Ning Zhang,Baoguo Sun,Yuping Liu,Shuqi Wang,Jie Sun
The flavor of deep-fried green onion oil (FO) changed significantly at 155 °C, with decreased fresh onion-like aroma and green scent, and increased savory and burnt notes. A total of 144 odorants were identified in FO samples. The total content of sulfur-containing odorants was highest at 155 ℃, while the total content of target furans and aldehydes/ketones peaked at 165 ℃. UFA accounted for approximately 95 % of the non-volatile fractions in FO, mainly in the form of PUFA . The oxidation degradation of UFA at high temperatures promoted the generation of small molecule aldehydes. Odorants in FO predominantly originated from reactions between sugars and amino acids , contributing to the enticing aroma.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Fanping Kong,Qian Zhang,Yuan Xie,Jiyue Ding,Hui Zhao,Zihan Zhang,Zequn Ma,Hailin Cong,Zilin Meng
Nanocarrier preparations could effectively improve the utilization rate of pesticides, and reduce pesticide loss. In this study, glyphosate (GLY)-loaded Mg Al layered double hydroxide (GLY@LDH) was synthesized via an in-situ method. Subsequently, GLY@LDH composite samples were prepared using a layer-by-layer self-assembly approach and modified with poly-L-aspartic acid (PASP) and chitosan (CS). XRD, FT-IR, SEM, and Zeta potential characterization confirmed that GLY was successfully loaded in the interlayer of LDHs and PASP/CS were successfully encapsulated on the surface of the composite sample. The release effect in different ionic solutions and soils was studied and analyzed. The release behavior conforms to the Ritger-Peppas kinetic model, and the release mechanism was ion exchange, which was further explored by means of XRD, SEM, and molecular simulation. The results of the anti-scouring experiment and contact angle measurement indicated that the layered self-assembly material enhanced the washing resistance of the material. The practical application effect of the sample was verified through a pot experiment. This study provides new insights into the simple preparation of pesticide-controlled release formulations that reduce leaching losses.
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PubMed
高影响因子
POLYMER
·
2021
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IF 4.43
Wenze Li,Mengyao Wang,Huan Luo,Yanhua Niu,Yajiang Huang,Guangxian Li
A high molecular weight (H M w ) PLLA has been blended with a low molecular weight (L M w ) PDLA and its two types diblock copolymers (PEG- b -PDLA and PDMS- b -PDLA), respectively, to investigate the stereocomplex (sc) and homochiral (hc) crystallization behaviors . The miscible PEG and immiscible PDMS segments with comparable molecular weight were introduced into PDLA by bulk ring-opening polymerization method. Compared to PLLA/PDLA, the introduction of both sequences could inhibit the crystallization kinetics of sc and hc crystallites of the blends due to the dilution effect of L M w segments. The formation of sc crystallites is prior to its hc counterparts. The shorter induction period of hc crystallites of PLLA/PEG-b-PDLA blend indicates that the miscible PEG segments could promote the mobility of PLLA chains. Due to the involving of PEG chains, the spherulite exhibits coarsen and irregular texture and its lamellae show sparse string-like structure. The microphase separation between PLA and PDMS segments as evidenced by SAXS results in the increased sc nuclei and different growth mode of hc crystallites. The phase separated microdomains weakened the competition between sc and hc crystallites, and the former acts as a template for repeated assembly of the latter. X-ray analysis also reveal that sc crystallites possess much thicker lamellar thickness than hc crystallites in PLLA/PDLA and PLLA/PEG-b-PDLA blends.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
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IF 4.952
Shan-Shan Zhang,Sen Guo,Zhao-Jing Zheng,Shao-Jing Liu,Yu-Fei Hou,Chi-Tang Ho,Nai-Sheng Bai
Volatiles in four Allium tenuissimum flower samples were detected and characterized by headspace-gas chromatography-olfactometry-mass spectrometry, and odor activity values. The key aroma compounds were determined by combining the sensory analysis with the omission and recombination experiments. The results illustrated that a total of 36 volatiles were identified and divided into six types, including sulfur-containing compounds, aldehydes, alcohols, ketones, nitrogen-containing compounds, and others. The sulfur-containing compounds and aldehydes were the two most abundant compounds in A. tenuissimum flower. The volatiles were quantified through authentic reference compounds, and the contents of these compounds in four A. tenuissimum flower samples were obtained. Five key aroma compounds of A. tenuissimum flower were confirmed as dimethyl disulfide, dimethyl trisulfide, linalool, 2-methylbutanal, and nonanal. Among these, the predominant compounds dimethyl disulfide and dimethyl trisulfide having strong onion and sulfur aroma, significantly contributed to the aroma of A. tenuissimum flower. The main aroma profiles of A. tenuissimum flower were onion, sulfur, flower, green and burnt. The closer the geographical location and environment of A. tenuissimum flower growth are, the more similar their chemical composition.
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INDUSTRIAL CROPS AND PRODUCTS
·
2020
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IF 4.244
Wenxiu Jiang,Xuejun Yu,Yuan Hui,Bowei Shen,Zhang Farong,Zhiquan Pan,Hong Zhou
Diosgenin is an important intermediate of steroid hormone drugs, green production of diosgenin with high yield is a key research issue. In this study, a convenient, efficient, and eco-friendly approach was developed to produce diosgenin from saponins in D. zingiberensis C. H. Wright (Curcuma longa L) using a magnetic solid acid. Fe 3 O 4 @SiO 2 -Pr-S-SO 3 H is a heterogeneous catalyst and was obtained by loading 3-(mercaptopropyl) trimethoxysilane on the surface of Fe 3 O 4 @SiO 2 , followed by sulfonation with chlorosulfonic acid. The magnetic solid acid catalyst was fully characterized by Fourier Transform-Infrared (FT-IR) spectra, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), and X-ray photoelectron spectroscopy (XPS), scanning electron microscope(SEM), transmission electron microscope (TEM), NH 3 -TPD, elemental mapping analysis. The effects of single factors, such as the solid acid mass, alcoholysis temperature, extraction time, and solvent volume on the diosgenin yield were analyzed. The extraction parameters for diosgenin were optimized by response surface methodology (RSM). The optimized values for the solid acid mass, solvent volume, extraction time, and temperature were 0.22 g, 7.8 mL, 7.4 h, and 100 °C, respectively. Under the optimized conditions, the maximum yield of diosgenin was 19.15 %. The solid acid reported in this work exhibited higher catalytic activity at a smaller dosage than traditional sulfuric acid and other solid acids. The alcoholysis products were identified by high-performance liquid chromatography-mass spectrometry (HPLC-MS). Alcoholysis catalysed by an as-prepared magnetic solid acid is clearly superior in terms of catalytic activity, dosage, and reusability. It is a promising strategy for the green production of diosgenin.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Fenfen Li,Pengcheng Du,Weiyu Yang,Danfei Huang,Shaoping Nie,Mingyong Xie
The intestinal epithelium is known as an important barrier to protect the body from harmful pathogens or toxic substance that may induce intestinal barrier injury. The aim of this study was to investigate the effects of polysaccharide from the seeds of Plantago asiatica L. (PLP) on nonylphenol (NP) induced intestinal barrier injury in vitro . Caco-2 cells were pretreated with PLP, or co-cultured with PLP and NP simultaneously, and cytotoxicity, LDH leakage, transepithelial electrical resistance (TEER), FITC-dextran flux and tight junction (TJ) proteins were conducted to evaluate the intestinal barrier function. The results suggested that PLP pretreatment or co-culture with NP could significantly attenuated NP induced Caco-2 cytotoxicity, suppressed LDH release, restored the TEER value and paracellular permeability of Caco-2 monolayers, which were attributed to enhancing the TJ protein expressions. In addition, PLP co-cultured with NP possessed better protective effects against NP induced cytotoxicity. This study indicated that PLP assuaged NP induced intestinal barrier injury by increasing TJ, and threw light on the development of a dietary supplementation for preventing exogenous toxic substances induced intestinal barrier injury or improving intestinal TJ barrier function.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Na Deng,Ziqian He,Ruixue Guo,Bisheng Zheng,Tong Li,Rui Hai Liu
The mechanisms of highland barley whole grain (BWG) with rich phenolics on obese db/db mice were investigated in this study. Oral consumption of BWG reduced food intake, body weight, organ/body weight indexes of liver and fat, levels of serum and hepatic lipids, liver injury, and oxidative stress. Furthermore, BWG recovered the disorder of cecal microbiota by augmenting the Bacteroidetes/Firmicutes ratio and Alistipes abundance and decreasing the abundances of Bacteroides and Desulfovibrionaceae to modulate lipid metabolism-related genes. BWG inhibited fatty acid biosynthesis via upregulating the phosphorylation of AMP-activated protein kinase α, while downregulating sterol regulatory element binding protein-1c, fatty acid synthase (FAS), and stearoyl-CoA desaturase 1 levels. BWG also significantly downregulated miRNA-122, miRNA-33, miRNA-34a, and miRNA-206 levels. Accordingly, BWG exhibited hypolipidemic potential through modulating cecal microbiota, AMPK/SREBP-1c/FAS pathway, and related miRNAs, triggering the alleviation of dyslipidemia. These findings suggested BWG as an effective candidate to ameliorate the symptoms of hyperlipidemia.
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MOLECULAR PHARMACEUTICS
·
2021
·
IF 4.939
Zhiting Sun,Xiaoxiao Wang,Jing Liu,Zhihong Wang,Weiwei Wang,Deling Kong,Xigang Leng
Photodynamic therapy (PDT) is promising for clinical cancer therapy; however, the efficacy was limited as an individual treatment regimen. Here, an approach synergistically combining PDT and nitric oxide (NO) gas therapy along with destruction of the tumor extracellular matrix (ECM) was presented to eliminate cancer. Specifically, the NO donor l-arginine (l-Arg) and the photosensitizer indocyanine green (ICG) were co-encapsulated in poly(lactic-glycolic acid) (PLGA) nanoparticles and then loaded into the poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL–PEG–PCL) hydrogel to develop an injectable, thermosensitive dual drug delivery system (PLGA@ICG@l-Arg/Gel). Significantly, reactive oxygen species (ROS) produced by PLGA@ICG@l-Arg/Gel under near-infrared (NIR) light irradiation could not only result in the apoptosis of cancer cells but also oxidize l-Arg to generate NO, which could suppress the proliferation of cancer cells. Moreover, ROS could further oxidize NO to generate peroxynitrite anions (ONOO–). ONOO– could activate matrix metalloproteinases (MMPs), which notably degraded collagen in ECM so as to damage the tumor microenvironment. PLGA@ICG@l-Arg/Gel significantly increased the antitumor efficacy against highly malignant 4T1 tumors in mice. Taken together, PLGA@ICG@l-Arg/Gel is a multifunctional platform that provides a novel strategy for cancer treatment with cascade amplification of the ROS oxidation effect, which holds great potential in clinical translation.
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PubMed
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Natural Product Communications
·
2020
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IF 0.468
Na Li,Ting Lv,Jie Pan,Chunhua Liu,Jia Sun,Yanyu Lan,Aimin Wang,Yongjun Li,Yonglin Wang,Yuan Lu
A simple, rapid, and selective ultra-performance liquid chromatography-mass spectrometry (MS)/MS method was established to investigate tissue distribution of 6 polyphenolic compounds of Polygonum orientale L. extract in normal and myocardial ischemia (MI) model rat tissues, including isoorientin, orientin, vitexin, quercitrin, astragalin, and protocatechuic acid. An Agilent Eclipse Plus C18 column was used. The mobile phase consisted of acetonitrile and water, both with 0.1% formic acid. Quantification was performed in negative ion multiple reaction monitoring mode. All the analysts had good linearity with r ≥ 0.9912. Accuracy ranged from 12.49% to −13.98% for the 6 compounds; within-day variation (precision) was ≤9.98% and interday precision was ≤11.88%. Extraction recovery of the analysts ranged from 80.55% to 99.92%; the matrix was 81.00%–98.73%. The analyst preparations were stable throughout. The 6 compounds were rapidly distributed in various tissues after oral administration, without accumulation over 12 hours. Compared with normal rats, distributions of 6 compounds in the heart, liver, spleen, lung, kidney, brain, stomach, and intestine in MI model rats were different from those in the normal group. The study provides an insight for further research of P. orientale L.
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高影响因子
ACS Applied Polymer Materials
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2020
·
IF 0
Yaxin Qiu,Juqun Xi,Qianru Wanyan,Defeng Wu
A biomass-derived and biocompatible poly(l-malic acid) (PMA) based material with shape memory effect (SME) was developed in this work. 1,8-Octanediol was used as the cross-linker to construct cross-linking networks with hydrophilic backbone chain of PMA, tuning the density of networks and their swelling capacities by controlling −COOH/–OH ratios. The solvent-induced SME of as-obtained cross-linked PMAs was activated under mild conditions in this way. Using the shape recovery levels of U-shaped samples as the probes, the mixed methanol/ethanol/water systems with various ratios could be easily discriminated because the cross-linked PMAs show different sensing capacities to different components. The mechanisms were then studied in terms of the solvent molecule–polymer interactions. This work provides an interesting approach of visible discrimination of the solvents with similar structures, or their mixtures with various ratios, and also opens applications of biocompatible shape memory polymers in biomedical and chemical industries.
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危险品化学品经营许可证（带存储）