CARBOHYDRATE POLYMERS
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2019
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IF 6.044
Jing Miao,Xi-qin Yang,Zhe Gao,Qian Li,Ting-ting Meng,Jia-ying Wu,Hong Yuan,Fu-qiang Hu
DrzBC and DrzBS (10-23 DNAzyme) could block the expression of HBV e- and s- gene respectively. But the application of 10-23 DNAzyme was limited owing to the lack of appropriate delivery vehicles. Chitosan oligosaccharide-SS-Octadecylamine (CSSO), a redox-responsive nano-sized polymeric carrier, could self-aggregate and bind with DNA by electrostatic interaction at proper mass ratio. Compared with the traditional commercial carrier Lipo2000, CSSO exhibited lower cytotoxicity, efficient cellular uptake by targeting cells, and rapidly DNA released in cytoplasm after escaping from endosomes. Including the same DNA concentration, Lipo2000/(DrzBC or DrzBS) showed maximum inhibitory rate on HBeAg (47.29 ± 1.86%) and HBsAg (33.58 ± 0.72%) secretion after 48 h incubation, and then both decreased. In contrast, HBeAg secretion inhibition by CSSO/DrzBC and HBsAg secretion inhibition by CSSO/DrzBS were up to 73.86 ± 1.77% and 67.80 ± 2.51% at 48 h, and further increased to 83.83 ± 2.34% and 76.79 ± 2.18% at 72 h, respectively. CSSO is a promising redox-responsive polymeric carrier for efficient anti-Hepatitis B Virus gene therapy.
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Journal of Materials Chemistry B
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2019
·Vol. 7
·期 11
·1882-1892
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IF 5.047
Chenmin Wang,Yuanman Yu,Han Chen,Shuang Zhang,Jing Wang,Changsheng Liu
Nutrients and oxygen are delivered mainly by blood vessels to nourish the cells and tissues in the body. Thus, biomaterials are processed by loading cytokines, such as vascular endothelial growth factors (VEGF), to facilitate angiogenesis in order to accelerate tissue regeneration. Nevertheless, the unpredictable biosecurity of exogenous cytokines is still a controversial issue for its clinical application. In this study, we constructed a kind of cytokine reservoir utilizing the binding affinity between heparin-like sulfate polysaccharide and endogenous growth factors. Two types of sulfated chitosan hydrogels, namely 6-O-sulfated chitosan (6-O-SCS) and 2-N,6-O-sulfated chitosan (2-N,6-O-SCS) hydrogels, were formed on the surface of the gelatin sponge matrix. The microstructure of the SCS-coated scaffolds is porous and interconnected, which is beneficial for cellular infiltration. Besides, human umbilical vein endothelial cells (HUVECs) can adhere and proliferate well on the surface of the scaffolds. Notably, sulfated chitosan-coated scaffolds exhibit an ability to capture VEGF in vitro & vivo, especially for the 2-N,6-O-SCS-coated scaffold. It is also verified by mice models that sulfated chitosan-coated scaffolds result in a concentrated VEGF microenvironment in specific domains as cytokine reservoirs and induce mass microvessels after implantation into subcutaneous tissues. As such, the sulfated chitosan-coated scaffolds served as VEGF reservoirs to accelerate angiogenesis and wound healing. This beneficial strategy may be applicable to in situ tissue regeneration by capturing more cytokines and promoting healing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Xiaoxiao Sun,Chao Liu,A.M. Omer,Wuhuan Lu,Shuxing Zhang,Xun Jiang,Hongjie Wu,Di Yu,Xiao-kun Ouyang
To develop relatively green and ecofriendly smart vehicles for colon-specific drug delivery, carboxymethyl cellulose (CMC) and chitosan (CS) pH-sensitive biopolymers were used in this study. To overcome the weaknesses of CMC carriers, such as poor mechanical performance and an explosive drug release, zinc oxide (ZnO) nanoparticles were incorporated into CMC beads and then coated with a CS layer via a self-assembly technique to form core–shell polyelectrolyte complexes. An anticancer drug, 5-fluorouracil (5-FU), used as a model drug, was loaded into ZnO/CMC/CS bio-nanocomposite beads. Fourier transform infrared spectroscopy , scanning and transmission electron microscopy , and thermogravimetric analysis were used to characterize the chemical structure, morphological changes, and thermal properties of the developed drug carrier, respectively. By studying their swelling and in vitro 5-FU release profiles under simulated gastrointestinal conditions, the pH sensitivity of the developed bio-nanocomposite hydrogel beads could be investigated. The obtained beads with reduced porosity could effectively encapsulate 5-FU and showed self-sustained release behavior depending on the concentrations of CMC, CS, and ZnO nanoparticles. The developed beads also demonstrated a capacity for biodegradation. The results indicated that the ZnO/CMC/CS bio-nanocomposite beads exhibited pH-sensitivity and could be applied efficiently as biodegradable carriers for colon-specific 5-FU delivery.
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DYES AND PIGMENTS
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2019
·
IF 4.018
Lu Zhang,Jun Wang,Yingying Zhang,Linjie Ke,Xiaowen Lin,Ziying Li,Haijun Chen,Yu Gao
The outcome of application of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in patients with non-small-cell lung cancer (NSCLC) always suffers from acquired drug resistance. Its combination with other therapies has been explored with promising synergistic results. Photodynamic therapy (PDT) is an established treatment modality for NSCLC, but the low tumor selectivity of currently available photosensitizers (PSs) hampered the wide clinical application of PDT. Here, we synthesized erlotinib modified chitosan (ECs) by click coupling and developed ECs/indocyanine green (ICG) self-assembled nanoparticles (GECs) for combined targeted chemo-photodynamic therapy. GECs showed oval-shaped particles with a diameter of 267.5 nm, a zeta potential of −15.2 mV, and considerable photostability. In vitro release experiment showed that erlotinib and ICG could be slowly released from GECs in acidic condition with lysozyme. GECs were capable of generating reactive oxygen species (ROS) for PDT under near-infrared laser irradiation . GECs showed synergistic molecular targeted and photodynamic therapeutic effects in inhibition of cellular growth and induction of apoptosis in NSCLC cells. This study demonstrated that the GECs could be a promising platform by combination of targeted chemo-photodynamic therapy for NSCLC treatment.
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DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
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2019
·
IF 2.367
Jing Zhou,Yuanyuan Chen,Mengmeng Luo,Fen Deng,Sen Lin,Wencan Wu,Guqiang Li,Kaihui Nan
Objective: This study was aimed to develop sustained drug release from levofloxacin (LF)-loaded chitosan (CS) microspheres for treating ophthalmic infections.Significance: Dual cross-linked CS microspheres developed by the spray-drying technique displays significantly higher level of sustained drug release compared with non-cross-linked CS microspheres.Methods: LF-loaded CS microspheres were prepared using the spray-drying technique, and then solidified with tripolyphosphate and glutaraldehyde as dual cross-linking agents. The microspheres were characterized by surface morphology, size distribution, zeta potential, encapsulation efficiency, and drug release profiles in vitro. The drug quantification was verified and analyzed by high-performance liquid chromatography (HPLC). The structural interactions of the CS with LF were studied with Fourier transform infrared spectroscopy. The effect of various influencing excipients in the formulation of the dual cross-linked CS microspheres on drug encapsulation efficiency and the drug release profiles were extensively investigated.Result: The microspheres demonstrated high encapsulation efficiency (72.4 ∼ 98.55%) and were uniformly spherical with wrinkled surface. The mean particle size was between 1020.7 ± 101.9 and 2381.2 ± 101.6 nm. All microspheres were positively charged (zeta potential ranged from 31.1 ± 1.32 to 42.81 ± 1.55 mV). The in vitro release profiles showed a sustained release of the drug and it was remarkably influenced by the cross-linking process.Conclusion: This novel spray-drying technique we have developed is suitable for manufacturing LF-loaded CS microspheres, and thus could serve as a potential platform for sustained drug release for effective therapeutic application in ocular infections.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Xiaoxiao Sun,Chao Liu,A.M. Omer,Li-Ye Yang,Xiao-kun Ouyang
A new pH-sensitive drug carrier based on alginate (Alg), chitosan (Cs), and kappa-carrageenan (kC) marine biopolymers was developed, comprising a dual-layered polyelectrolyte complexes microbead structure. Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) were used to investigate the structures, thermal stability , and morphological changes, respectively, of the microcapsules we developed. In the intestinal environment (pH 7.4), the stability and swelling of Alg microbeads were enhanced after generation of the dual-layered polyelectrolyte complexes. The in vitro release profiles of 5-fluorouracil under simulated GI conditions were also examined. At gastric pH (1.2), the cumulative 5-FU release percentage was decreased by introduction of the additional kC layer from 14% in Alg/Cs microbeads to 7% in the dual-layered Alg/Cs/kC microbeads. Additionally, the release profiles were greatly improved under simulated intestinal and colon conditions. Finally, the microbeads were all biodegradable and showed no cytotoxicity in Chang liver cells. Our results clearly indicate that the dual-layer pH-sensitive Alg/Cs/kC microbeads could be used effectively for the targeted delivery of anticancer drugs to the colon.
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Nanoscale
·
2019
·Vol. 11
·期 38
·17736-17745
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IF 6.97
Qian Zhu,Fushan Wang,Fengwei Zhang,Zhengping Dong
The direct oxidation of alcohols to esters through a green and cost-effective strategy is a fascinating chemical synthesis route. In this study, an environmentally friendly N-doped porous carbon encapsulated Co-based nano-catalyst was prepared via a simple carbonization procedure, utilizing renewable chitosan, accessible dicyandiamide and low-cost Co(OAc)2 as co-precursors. The obtained Co@NC-2-T catalysts were successfully used in selective oxidation of aromatic alcohols with methanol to esters under atmospheric reaction conditions. The Co@NC-2-900 catalyst (added with 2 g dicyandiamide and pyrolyzed at 900 °C) shows optimal activity and applicability and can also be reused at least six times in the oxidative esterification of aromatic alcohols with excellent stability. The presence of superoxide anion radicals in the current catalytic system was detected by the EPR method, and a possible mechanism of alcohol oxidation to ester was proposed on this basis. Thus, this study provides a facile, eco-friendly, and highly efficient catalytic system for oxidative esterification of alcohols.
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Polymers
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2019
·Vol. 11
·期 9
·1417
·
IF 3.164
Wei Wang,Zufeng Xiao,Chaofan Huang,Kewang Zheng,Yin Luo,Yumin Dong,Zitong Shen,Wei Li,Caiqin Qin
Chitosan microspheres modified by 2-pyridinecarboxaldehyde were prepared and used in the construction of a heterogeneous catalyst loaded with nano-Cu prepared by a reduction reaction. The chemical structure of the catalyst was investigated by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and X-ray Photoelectron Spectroscopy (XPS). Under mild conditions, such as no ligand at room temperature, the catalyst was successfully applied to catalyze the borylation of α,β-unsaturated receptors in a water-methanol medium, yielding 17%–100% of the corresponding β-hydroxy product. Even after repeated use five times, the catalyst still exhibited excellent catalytic activity.Keywords:chitosan microspheres;copper;organoboron compounds
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CARBOHYDRATE POLYMERS
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2019
·
IF 6.044
Shuangyu Xiong,Luminita Marin,Lian Duan,Xinjian Cheng
Nitroaromatic compounds, especially 2, 4, 6-trinitrophenol, have strong biological toxicity and explosive risks, so the detection of 2, 4, 6-trinitrophenol exhibit importantly practical and scientific significance. In this work, three fluorescence functionalized chitosan CNS 3 , CNS 4 and CNS 5 were prepared using chitosan as matrix. When 2, 4, 6-trinitrophenol (TNP) and/or p-nitrophenol (4-NP) was present in spot, these fluorescent chitosan sensors produced notable fluorescence quenching. It renders the chitosan sensing ability to detect TNP and 4-NP selectively and sensitively. The sensing mechanism is investigated as well. When introduced electron-rich moieties to the fluorescent chitosan, the sensitive detecting ability could be obtained. Excellent recognition ability could reach as low as 0.28 μM. The fluorescence fictionalization cause slight influence to the gel performance of chitosan.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yang Liu,Lishuang Hu,Bo Tan,Jingru Li,Xiaohui Gao,Yaning He,Xifeng Du,Wei Zhang,Weili Wang
Dextran-chitosan (DC) macromolecule resin was synthesized by ultrasonic heating and applied to adsorb various heavy metal ions (Cu 2+ , Co 2+ , Ni 2+ , Pb 2+ , Cd 2+ ). The morphology and structure of the samples were characterized by various testing methods. The effects of five factors on the adsorption properties were studied. The adsorption kinetics , thermodynamics and isotherm models were discussed theoretically. The results show that the adsorption of heavy metal ions by DC resin is a spontaneous single molecule chemical adsorption, and the adsorption capacities of DC resin for Cu 2+ , Co 2+ , Ni 2+ , Pb 2+ and Cd 2+ were 342 mg g −1 , 232 mg g −1 , 184 mg g −1 , 395 mg g −1 , and 269 mg g −1 , respectively at 20 °C, pH = 7 and adsorbent dose is 0.01 g. In addition, DC resin adsorbent has good reusability.
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SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Yonghai Song,Jiajia Han,Lijuan Xu,Longfei Miao,Canwei Peng,Li Wang
Dopamine (DA) is a nerve-transducing chemical that helps cells transmit pulses. Too high or too low concentration of DA is detrimental to human health. Therefore, finding a fast, efficient and sensitive method for detecting DA is of great significance. Here, a DA-imprinted chitosan (CS) film/ZnO nanoparticles (NPs)@carbon (C)/three-dimensional kenaf stem-derived macroporous carbon (3D-KSC) was prepared for electrochemical detection of DA. The DA-imprinted CS film/ZnO NPs @C/3D-KSC was prepared by growing zeolite imidazole framework-8 (ZIF-8) on 3D-KSC, carbonizing under N 2 atmosphere, electrodepositing AA-CS on ZnO NPs@C/3D-KSC and eluting template AA molecules. The results showed that ZIF-8 derived porous ZnO NPs@C nanospheres were arranged evenly on 3D-KSC surface to form a monolayer. The diameter of nanospheres was approximately 500–600 nm, and the nanospheres were composed of ZnO NPs embedded in porous carbon. A layer of CS with DA-imprinted pores was covered on the ZnO NPs@C nanospheres, so the DA-imprinted CS film/ZnO NPs@C/3D-KSC integrated electrode exhibited better selectivity for DA detection. The linear range was 0.12 nM-152 μM, the detection limit was 0.039 nM, and the sensitivity was 757 μA mM −1 cm -2 . The DA-imprinted CS film/ZnO NPs@C/3D-KSC integrated electrode also showed higher stability.
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CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Yanjun Huang,Hailing Wu,Taikang Shao,Xia Zhao,Hong Peng,Yuefa Gong,Huihai Wan
A novel composite adsorbent of Ca-DTCS/ALG bead was prepared by immobilization of DTPA-modified chitosan micro-gels (DTCS) into the alginate matrix. The copper sorption properties of Ca-DTCS/ALG beads were tested and compared with the other two adsorbents of DTCS powder and plain alginate (ALG) beads. In addition, the mechanisms of copper sorption on the adsorbents were investigated by FTIR and XPS analysis. Similar to DTCS powder, Ca-DTCS/ALG beads exhibited high copper removal efficiency in strongly acidic solutions, whereas calcium, sodium and aluminum salts had little effect on its copper adsorption capacities. The maximum adsorption capacities of Ca-DTCS/ALG and DTCS were 107.5 and 106.0 mg g −1 at pH 3.0, respectively, which were close to the values predicted by the Sips model. However, the maximum adsorption capacity of ALG beads predicted by the Sips model was much higher than its experimental one (101.1 mg g −1 ). The copper adsorption equilibrium by DTCS powder was achieved within 1 min, nevertheless, ALG and Ca-DTCS/ALG gel beads reached the adsorption equilibrium within 500 min and 1440 min, respectively. Moreover, Ca-DTCS/ALG gel beads showed good reusability, but DTCS powder was not easily reused due to its notable swelling property. Furthermore, the efficiency of copper recovery by Ca-DTCS/ALG beads in the simulated electroplating wastewater at the copper concentration of 1–5 mg L −1 was much higher than that of DTCS and ALG beads. These results reveal that immobilization of DTCS into the alginate matrix greatly improves the adsorption and separation performance of the adsorbent.
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