HUMAN REPRODUCTION
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2022
·Vol. 37
·期 7
·1394-1405
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IF 6.353
Liu Ruyi,Yan Zheng,Fan Yong,Qu Ronggui,Chen Biaobang,Li Bin,Wu Ling,Wu Haibo,Mu Jian,Zhao Lin,Wang Wenjing,Dong Jie,Zeng Yang,Li Qiaoli,Wang Lei,Sang Qing,Zhang Zhihua,Kuang Yanping
STUDY QUESTIONAre there new genetic factors responsible for male infertility with normal sperm quantity and morphology?SUMMARY ANSWERWe identified the bi-allelic variants in KCNU1 and confirmed it a novel pathogenetic gene for male infertility mainly due to impaired sperm acrosome reactions (ARs).WHAT IS KNOWN ALREADYUntil now, the underlying genetic determinants for male affected individuals exhibiting normal sperm quantity and morphology have been largely unknown. Potassium/calcium-activated channel subfamily U member 1 (KCNU1) is a sperm-specific potassium channel. The Kcnu1 null mutation in male mice causes infertility due to the impaired progressive motility and AR.STUDY DESIGN, SIZE, DURATIONWe recruited a cohort of 126 male infertility individuals with typical asthenospermia or fertilization failure and focused on two infertile males from two consanguineous families from 2015 to 2020; whole-exome sequencing and homozygosity mapping were performed. We identified a homozygous missense variant (c.2144A>G, p.His715Arg) and a homozygous donor splice-site variant (c.1295 + 3A>C, p.Val405Glyfs*8) in KCNU1. Then, we generated a knock-in (KI) mouse model in September 2020 and have now carried out functional studies and possible treatment strategies.PARTICIPANTS/MATERIALS, SETTING, METHODSThe affected individuals with infertility were recruited from the Shanghai Ninth Hospital affiliated to Shanghai Jiao Tong University. Genomic DNA from the affected individual was extracted from peripheral blood. Whole-exome sequencing, homozygosity mapping and in silico analyses were used to screen and identify KCNU1 variants, and the variants were confirmed by Sanger sequencing. We used C57BL/6N mouse to construct KI mouse model to mimic the reproductive phenotype in vivo. We performed functional experiments by western blotting, AR assay and immunofluorescent Staining. Finally, we performed IVF and ICSI to explore the treatment strategies.MAIN RESULTS AND THE ROLE OF CHANCEWe identified a homozygous missense variant (c.2144A>G, p.His715Arg) and a homozygous donor splice-site variant (c.1295 + 3A>C, p.Val405Glyfs*8) in KCNU1 in two infertile males. We demonstrated that the splice-site variant affected normal alternative splicing of KCNU1, thus leading to the loss of function of KCNU1. Meanwhile, the missense pathogenic variant reduced the KCNU1 protein levels in sperm of both the affected individual and the KI mouse model, resulting in impaired ARs and male infertility. Intracytoplasmic sperm injection was able to rescue the deficiencies.LARGE SCALE DATAN/A LIMITATIONS, REASONS FOR CAUTIONThe exact molecular mechanism of KCNU1 and pathways need to be further explore in the future.WIDER IMPLICATIONS OF THE FINDINGSThis is the first report that establishes a causal relationship between KCNU1 deficiency and male infertility, confirming the critical role of KCNU1 in human reproduction. Our findings expand our knowledge of the genes that play critical roles in the human sperm AR and provide a new genetic marker for infertility.STUDY FUNDING/COMPETING INTEREST(S)This work was supported by the SHIPM-pi fund no. JY201801 from the Shanghai Institute of Precision Medicine, Ninth People’s Hospital Shanghai Jiao Tong University School of Medicine, the National Natural Science Foundation of China (81725006, 81771649, 81822019, 81771581, 81971450, 81971382, 82001538 and 82071642). The authors declare no conflict of interest.TRIAL REGISTRATION NUMBERN/A.
研究领域:
遗传学
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生殖生物学
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分子生物学
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DOI
PubMed
高影响因子
Journal of Materials Research and Technology-JMR&T
·
2020
·Vol. 9
·期 4
·9142-9152
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IF 5.289
Jian-bo Xie,Tian Fan,Zhi-qi Zeng,Han Sun,Jian-xun Fu
This work investigates the effect of Bi addition on the formation of sulfide inclusions, and also aims to improve the machinability and mechanical properties of type 0Cr18Ni9 stainless steel. Smelting experiments as well as machining and tensile tests were carried out in various low and high Bi content steels. Results show that the forms of Bi-MnS inclusions were: isolated particle, cavity-bridge link, semi- and complete encapsulation. The forms of MnS inclusions were found as: mushroom or rod-like, long strip, spherical, and spindle shape. The normalized frequency of 0.065, corresponding to the inclusion area above 50 μm 2 , was the greatest at 2300 ppm Bi, reflecting an increase in the number of larger-sized inclusions. The averge hardness of steel matrix and the inclusions at 0 ppm Bi was 179 and 166 kgf/mm 2 , while that after Bi addition was 154∼168 and 155∼166 kgf/mm 2 , respectively. The Bi addition to steel improved the surface finish of the steels after machining and increased the ductility of steel, but had little effect on the yield strength of steel.
研究领域:
材料科学
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冶金学
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机械工程
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DOI
高影响因子
Advanced Fiber Materials
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2025
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IF 21.3
Li Xiaoyan,Guo Zhiguang,Ji Yating,Xie Yu,Du Peibo,Wang Jie,Xue Yunfan,Xu Bi,Cai Zaisheng
Integration of radiative cooling and heating within a single textile is essential for effectively maintaining human thermal comfort and reducing energy consumption. However, most existing radiative thermal-management textiles depend either on complex micro/nanostructure fabrication techniques or on the simple stacking of cooling and heating layers, both of which substantially limit their scalability and long-term durability in practical applications. Here, we report a scalable dual-mode thermal management textile with biomimetic asymmetric solar confinement (ASCT), achieved through the design of asymmetric micro/nano-structures guided by controlled fiber-orientation alignment. ASCT demonstrates excellent solar modulation capability, with the high solar confinement (HSC) side exhibiting a solar absorptance of 97%, while the low solar confinement (LSC) side maintains a significantly lower absorptance of only 4.9%. Additionally, the mid-infrared (MIR) emissivity contrast between the two sides reaches up to 40%. Compared to conventional textiles, ASCT significantly enhances the thermal modulation range by up to 15 °C. More importantly, ASCT has mechanical robustness, breathability, softness, and durability tailored for wearable applications. This work offers a novel path to the structural design and scalable fabrication of textiles, which is expected to accelerate their advancement and practical deployment. Graphical abstract
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2017
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IF 3.133
Fengyu Kong,Wei Ning,Anding Wang,Yan Liu,Mingliang Tian
Bi is a semimetal with unusual and attractive electronic properties. However, synthesis of nanoscale 0-2D Bi single crystal with good dispersity and quality without surfactants and substrates is still a challenge. In this paper, a novel, simple and low-cost solvothermal method for synthesis of 0-2D nanoscale Bi at mild conditions and without a surfactant/substrate is reported. By using Bi(NO 3 ) 3 ·5H 2 O or NaBiO 3 ·2H 2 O as Bi sources and environmental friendly C 3 H 8 O 3 as solvent, we synthesized nanospheres , nanowires and nanoribbons characterized with monodispersity, high purity and crystallinity. The growth mechanism of the 0-2D nanostructure was discussed. It is found that the Bi source, concentration and solvent significantly influences the morphology and size. Single crystal free-standing nanoribbon has large magnetoresistance up to 540% at 100 K. This novel approach can be applied in synthesis of nanostructured Bi single crystals and the large-scale fabrication of some other nanostructured semimetals.
研究领域:
材料科学
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纳米技术
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
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IF 3.131
Yumei Peng,Lixia Qin,Jiefei Liu,Shi-Zhao Kang,Guodong Li,Xiangqing Li
Here, by a simple one-step in situ chemical reduction method, the Ag x Cu y alloy was tailored on the surface of mesoporous TiO 2 (M-TiO 2 ), therefore, a series of bi-functional mesoporous TiO 2 nanohybrids (M-TiO 2 /Ag x Cu y ) for both surface enhanced Raman scattering (SERS) detection and photocatalytic degradation of ampicillin (AMP) were achieved. The composition and structure of the nanohybrids were well characterized using XRD, TEM, XPS and HRTEM-EDS measurements. Results indicated that Ag x Cu y alloy was mainly attached around the pores of M-TiO 2 which may generate more active sites than nonporous P25−Ag−Cu. Thus, the M-TiO 2 /Ag x Cu y nanohybrids could be used as efficient SERS substrates to sensitively monitor the AMP in water with lower detection limit (˜ 1 ppt) than P25−Ag−Cu. Besides, the M-TiO 2 /Ag 3 Cu 1 nanohybrid also showed higher photocatalytic activities for the degradation of AMP under UV irradiation over the M-TiO 2 /Ag, the M-TiO 2 /Cu and the P25−Ag−Cu. This work provided a simple and effective approach to synthesize bi-functional nanohybrids for detection and degradation of organic contaminants in wastewater.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
ACS Applied Nano Materials
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2019
·
IF 0
Yan Cheng,Navid Hussain Shah,Jian Yang,Kai Zhang,Yanyan Cui,Yaling Wang
A multicomponent Bi-based Z-scheme photocatalyst, BISB, was developed through a hydrothermal method. In this sample, two semiconducting nanostructures, BiOI and Bi2S3, were linked together with the metal Bi nanoparticle. As compared with BiOI (BI) and heterojunction BiOI/Bi2S3 (BIS) samples, the BISB had optimal Rhodamine B (RhB) photodecomposition performance. High efficiency separation of photoexcited electrons and holes, decreased band gap energy, extended specific surface area, and promotional redox potential were key factors for enhancing the photocatalytic activity of the prepared sample. The photoexcited holes were the key active radicals during the RhB photodecomposition process over the BISB catalyst, while ·O2¯ was also involved to a smaller extent. Additionally, the photocatalyst demonstrated excellent reusability and stability during the photoreaction process, suggesting that the BISB sample could be a candidate for water purification applications.
研究领域:
材料科学
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环境科学
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产品关联
DOI
Journal of Physical Chemistry C
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2017
·
IF 4.536
Zhan Zhang,Lixia Ren,Hong Yan,Shujin Guo,Shuanhu Wang,Min Wang,Kexin Jin
Single crystals of heterovalent Bi-doped CH3NH3PbCl3 perovskite have been successfully grown through the inverse temperature crystallization method. Bandgap narrowing of 300 meV (a 55 nm red-shifting absorption edge) is obtained for the nominal 20% Bi-doped crystal with the host structure and the energy at the top of the valence band invariable. It is observed that the contact between the Au electrodes and single crystals transforms from Ohmic to Schottky, and the conductivity increases with increasing Bi doping level. By contrast, the Bi-doped CH3NH3PbCl3 thin films are also prepared, and the similar bandgap narrowing is found, although the narrowing degree is less than that in single crystals. This work has a comprehensive understanding of Bi-doped single crystals and thin films, providing further optoelectronic applications for these promising solution-processed hybrid perovskite semiconductor materials.
研究领域:
材料科学
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光学
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DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2017
·Vol. 33
·期 6
·929-933
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IF 1.024
Bu Luxia,Liu Daying,Wei Zhen,Ma Zhanlin
The preparation and characterization of homogeneous and colorless ionic liquid analogs(ILAs) containing choline chloride(ChCl), malonic acid and bismuth chloride were investigated. The structure of the above mixture was preliminarily analyzed, and then the physicochemical properties including viscosity, conductivity and density were investigated as functions of temperature and composition. Additionally, the thermal stability of the ILAs and the electrochemical behavior of Bi3+ ions in the medium were studied. Structure analysis reveals that the synthesis of deep eutectic solvents(DESs) is closely related to the formation of hydrogen bonds between malonic acid and ChCl, with their viscosity, conductivity and density being greatly dependent on the temperature and composition. Thermogravimetric analysis demonstrates that the ILAs are very stable between room temperature and 398.15 K. And the electrochemical experiments indicate that the bismuth films can be successfully deposited from this ILAs and the deposition mechanism of bismuth is a rather irreversible process. The surface morphology of bismuth films deposited on Cu substrates was very compact and smooth. Thus, ChCl-malonic acid DESs were shown to be a good alternative to aqueous solvents for various deposition-related applications.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
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2013
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IF 3.777
Ronghua Liu,Lina Jin,Dan Lu,Gang Hu,Long He,Jun Shan
In this paper, inspired by the cubic bi-continuous gyroid structure self-assembled by block copolymers, we start with the application of a novel and more facile approach, compared to the tedious synthetic procedures of block copolymers, to prepare the bi-continuous phases structured nanocomposite electrolytes. The bi-continuous phase structure is composed of a hard phase of the electrospun poly vinylidene fluoride (PVDF) non-woven scaffold providing the mechanical support for the electrolyte membranes and another soft phase built up then by the infiltration of polyethylene oxide (PEO) electrolyte into the PVDF scaffold membrane to be the ionic conducting phase. Therefore, the nanocomposite polymer electrolytes of the bi-continuous phase structure are prepared with addition of the different amounts of TiO 2 nanoparticles into the PEO electrolyte (containing LiClO 4 ). The nanocomposite electrolyte membranes have systematically been characterized by scanning electron microscopy (SEM), differential scanning calorimeter (DSC), X-ray diffraction (XRD), and tensile test. The tensile results indicate that the infiltration of PEO phase into the PVDF scaffold can greatly improve the elongation of the electrolyte membrane at break, but the tensile strength of the electrolyte membrane is substantially dependent on the electrospun PVDF scaffold. The ionic conductivity measurements show that addition of TiO 2 nanoparticles with an appropriate amount of ca . 10 wt% enhances the ionic conductivity of the nanocomposite electrolytes to 7.60 × 10 −5 S cm −1 at 60 °C, compared to 8.33 × 10 −6 S cm −1 at 60 °C of the PEO–LiClO 4 electrolyte. The effect of TiO 2 nanoparticles on the enhancement of Li + ion conductivity has been investigated by attenuated total reflectance-Fourier transform infrared spectra (ATR-FTIR), and the results show that a competitive interaction between TiO 2 nanoparticles and Li + ions weakens to some extent the existing complexing action of ether-O…Li + , allowing Li + ions faster transfer. However, we have found by high-resolution transmission electron microscopy (HRTEM) that the real dispersion state of TiO 2 nanoparticles in the nanocomposite electrolytes is in the form of large aggregates instead of the individual primary nanoparticles, which would tremendously depress the surface effect of the individual nanoparticles and greatly impact on the enhancement of the ionic conductivity.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2019
·
IF 14.229
Fang Li,Wei Zhao,Dennis Y.C. Leung
Photoelectrocatalytic hydrogen production is a clean and sustainable way to combat energy crises and environmental issues. The performance of BiVO 4 which acts as a promising photoanode could be effectively enhanced by loading with Bi nanoparticles. In this study, a viable method to fabricate Bi/BiVO 4 electrode was proposed to enhance hydrogen production through a photoelectrocatalytic reaction. The results indicated that Bi nanoparticles loaded under optimal condition could obviously increase the photocurrent density of the BiVO 4 electrode from 0.9 mA cm −2 to 2.0 mA cm −2 under visible light illumination. Furthermore, the photoelectrochemical hydrogen production efficiency could have a 2.5-fold improvement with the addition of 20 ppm phenol. The possible mechanism of the enhanced performance and the function of phenol in the overall reaction was discussed.
研究领域:
材料科学
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能源化学
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产品关联
DOI
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 12
·5499-5505
·
IF 6.97
Hongjing Wang,Yinghao Li,Dandan Yang,Xiaoqian Qian,Ziqiang Wang,You Xu,Xiaonian Li,Hairong Xue,Liang Wang
Electrochemical reduction of N2 represents a very attractive approach for sustainable NH3 production at environmental temperature and pressure. The design of highly branched Pd-based nanoarchitectonics is very important for the electrocatalytic N2 reduction reaction (NRR). Herein, we propose a very simple synthetic method for direct fabrication of unique bi-metallic PdRu nanorod assemblies (PdRu NRAs) with high yield in an aqueous solution. Owing to their branched shape together with bi-metallic compositions, the self-supported PdRu NRAs assembled with staggered nanorods show high catalytic activity and superior durability for the NRR. The presented direct synthetic strategy is very valuable for the design of active branched metallic catalysts for the NRR.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2023
·
IF 7.2
Yuan Zhao,Qingshuang Wang,Dongming Yin,Shouliang Li,Chunli Wang,Long Liang,Shaolei Zhao,Chunmin Zhang,Limin Wang,Yong Cheng
The high dehydrogenation temperature of α-AlH 3 has always been an important factor hindering its development. In order to solve this problem, we synthesize Bi-MOF by hydrothermal method and burn it under inert gas to obtain Bi@C, which is used as catalyst ( x Bi@C, x = 3, 5 and 7 wt%) to improve the hydrogen desorption performance of α-AlH 3 . The dehydrogenation onset temperature of α-AlH 3 +5 wt% Bi@C drop to 80.4 °C, which is reduced by 43.4% compared with pure α-AlH 3 . At 120 °C, it can provide a stable hydrogen capacity of 7.45 wt%. In contrast, pure α-AlH 3 releases only 6.66 wt% hydrogen at the same time. The density functional theory calculations further indicate that the existence of Bi@C catalyst can make the Al–H bond length increase, more conducive to the release of hydrogen. The results show that the synergistic effect of Bi and porous carbon in Bi@C materials can improve the hydrogen desorption kinetics of α-AlH 3 , providing a good prospect for the application of α-AlH 3 in hydrogen storage .
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高影响因子
危险品化学品经营许可证（带存储）