BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Qian Dong,Chun-xiao Gong,Ge-liang Xie,Guo-qiang Zhu,Zhen Fang
Freeze pretreatment combined with alkaline-hydrothermal method of rice straw for enzymatic hydrolysis was studied. Crystallization stress in the rice stem pores caused by water freezing at −20– −40 °C was modeled to illustrate the destruction mechanism. The stress was calculated as 22.5–38.3 MPa that were higher than the tensile yield stress of untreated stems (3.0 MPa), indicating ice formation damaging pore structure. After freeze at −20 °C, rice straw was further hydrothermally treated at 190 °C with 0.4 M Na 2 CO 3 , achieving 72.0 % lignin removal and 97.2 % cellulose recovery. Glucose yield rose to 91.1 % by 4.3 times after 24 h hydrolysis at 10 FPU loading of Cellic®CTec2 cellulase. The specific surface area of rice straw was 2.6 m 2 /g increased by 1.2 times after freeze. Freeze combined with alkaline-hydrothermal treatment is a green and energy-efficient method for improving enzymatic hydrolysis.
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高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Kehan Zhu,Zhen Yao,Tianyi Gu,Xianhao Jiang,Jianqin Zhou,Duxin Li
Swollen culms of Zizania latifolia possess a peculiar flavor, nutritional value and health-promoting effects. This study investigated the wound healing ability of polysaccharides isolated from Zizania latifolia (ZLP). Through in vitro assays, ZLP demonstrated negligible cytotoxicity and promoted the migration and phagocytosis of Staphylococcus aureus by macrophages where ZLP-2 had a significantly greater effect. Moreover, ZLP-2 has a higher purity and molecular weight, an integral and large lamellar morphology, and a larger particle size in solution, suggesting its ability to form films. Thus, ZLP-2 was fabricated as a gel formulation for topical application. The in vivo results showed that the ZLP-2 gel had significantly greater effects than did the autonomous healing process according to quantitative analysis of the wound closure area. The ZLP-2 gel decreased the levels of TNF-α and IL-6 in wounded tissue. Furthermore, the quantification of the molecular indexes via qRT-PCR revealed that the levels of tnf-α, tgf-β, mcp-1, ifn-γ, inos, and vegf in the treated group increased during the inflammatory and proliferation stages but decreased after wound closure. These results suggest that ZLP-2 has great potential in pharmaceutical applications for wound care.
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高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Haizhao Song,Jing Lu,Qiang Chu,Rou Deng,Xinchun Shen
BACKGROUND Because of their diverse biological activities, polysaccharides derived from Tremella fuciformis have received growing attention. This study aimed to investigate the structural characterization of a purified polysaccharide (designated as PTP-3a) derived from T. fuciformis and explore its interaction with gut microbiota in vitro . RESULTS The findings revealed that PTP-3a had a molecular weight of 1.22 × 10 3 kDa and consisted of fucose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.271:0.016:0.275:0.400:0.038. The primary linkage types identified in PTP-3a were 1,3-linked-manp, 1,4-linked-xylp and 1,2,3-linked-fucp, with corresponding ratios of 0.215:0.161:0.15. In addition, PTP-3a demonstrated notable thermal stability and exhibited a triple-helical structure. Moreover, following in vitro fermentation for 48 h, PTP-3a was efficiently utilized, resulting in a reduction in carbohydrate levels, the production of short-chain fatty acids (SCFAs) and pH adjustment. Furthermore, during in vitro fecal microbial fermentation, PTP-3a decreased the relative abundance of Firmicutes while increasing the proportions of Bacteroidetes and Proteobacteria, resulting in a significantly reduced Firmicutes/Bacteroidete s ratio. Additionally, PTP-3a stimulated the growth of beneficial bacteria such as Parabacteroides merdae , Gordonibacter pamelaeae , Bifidobacterium pseudolongum and Parabacteroides distasonis . Importantly, a strong correlation was observed between the production of SCFAs and specific microorganisms. CONCLUSION These findings suggested that PTP-3a has potential as a prebiotic for modulating the gut microbiota. © 2024 Society of Chemical Industry.
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2024
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IF 10.8
Lihong Gan,Jin Zhang,Yangtao Wu,Zhuo Chen,Zhenyu Zhao,Shihong Lin,Yi Jiang
Polyelectrolyte multilayer (PEM) membranes, with advantageous features of versatile chemistry and structures, are driving the development of advanced nanofiltration (NF) membranes with exceptional performance. While developing a printing method holds great promise for the eventual mass production of these membranes, reports on the printing method and the underlying mechanisms of membrane formation are currently scarce. Herein, we develop an aerosol-assisted printing (AAP) system for fabricating PEM NF membranes with highly tunable separation characteristics. Our study unveils the three stages of membrane formation from assembly of polyethylenimine (PEI) and poly(sodium 4-styrenesulfonate) (PSS): aerosol deposition, single PE layer formation, and PEM assembly. The droplet deposition is governed by inertial impaction, and the deposited PEs migrate/entangle to form a single PE layer. The thicknesses of the PE layer and PEM exhibit linear growth as the number of printing scan increases. Furthermore, PE interdigitation forms an effective polymeric network barrier, which increases the resistance to solute and water transport. By manipulating the PE deposition mass and layering, PEM membranes with tunable pore radii (0.40–0.56 nm) and water permeability (5–60 L·m–2·h–1·bar–1) were obtained for various water treatment applications, ranging from micropollutant removal to humic acid filtration. Our study offers valuable mechanistic insights into the PEM formation and precise structural adjustment via printing, thus facilitating scalable manufacturing and widespread applications of the PEM NF membranes.
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高影响因子
FOOD CONTROL
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2024
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IF 5.6
Luyao Zhao,Jin Liu,Bing Bai,Yongpeng An,Yukun Yang,Xiaowen Wang,Xu Jing
Herein, we established a green, rapid, and sensitive method to analyze ochratoxin A (OTA) in cereals based on modified QuEChERS and enzyme-linked immunosorbent assay (ELISA). Lignocellulose is a renewable material mainly consisting of cellulose, hemicellulose, and lignin. In the modified QuEChERS, a natural deep eutectic solvent synthesized from glucose and levulinic acid derived from cellulose and hemicellulose was used as an extraction solvent to replace traditional organic solvents. Furthermore, modified lignin was used as an adsorbent to purify the extraction solution. In the magnetic bead-based ELISA, the effervescent reaction accelerated the immune competition. Additionally, magnetic beads were used to shorten the incubation and washing time, whereas gold nanoparticles were used to amplify the optical signal. This method has been successfully applied to the analysis of OTA in cereal samples. The limits of detection were 0.68–0.83 μg kg −1 . This pretreatment and detection strategy has potential application in analyzing mycotoxins.
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DESALINATION
·
2024
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IF 8.3
Gao Xuerui,Li Ping,Qin Yuan,Bai Chengling,Gu Zhengyang,Yu Shuili
The negative rejection of certain solutes is an interesting phenomenon often observed in nanofiltration (NF) process, which presents an ideal solute separation effect. In this study, the NF membrane pore size, electric charge property and influent ions composition were controlled to investigate the rule and mechanism of negative rejection phenomenon during SO 4 2− /Cl − mixed salt NF. The results show that the changing of the membrane pore diameter and charge will significantly affect the NF selectivity to SO 4 2− /Cl − , then affect the negative rejection phenomenon. Selective efficiency of Cl − /SO 4 2− can be up to 156.04–283.20 for the negative charged NF membrane with an average pore diameter of 0.62–0.68 nm, and the Cl − was negatively rejected under many influent conditions with the lowest rejection rate of −252.36 %. The substitution of Na + for Mg 2+ and the increase of SO 4 2− /Cl − ratio are beneficial to improve selectivity. The Donnan Steric Pore Model with dielectric exclusion model was used to calculate the rejection curve which is consistent with the experimental results. The analysis of NF mechanism shows that the negative rejection of Cl − is caused by the extra transfer power derived from the NF selectivity to Cl − , which is a compensation for the higher rejection rate of SO 4 2− .
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高影响因子
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