SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
·
IF 4.3
Jiangyue Ning,Xingyan Bao,Haotian Chen,Zelong Yan,Li Ding,Chang Shu
Thrombin plays a critical role in hemostasis and hemolysis, and is a significant biomarker for blood-related diseases. Detection and inhibitors screening of thrombin are essential in medical research. In this study, we developed a fluorescent sensor based on the interaction between quantum dots (QDs) and fibrinogen (Fib) for thrombin detection and its inhibitors screening. Upon the presence of thrombin, the fibrinogen of soluble QDs-Fib were converted into insoluble fibrin precipitate, causing a change of fluorescence intensity in the supernatant. Under optimized conditions, our method exhibited an excellent linearity (R 2 ≥0.99) over the range of 2∼100 U/L with a limit of detection (LOD) as low as 0.29 U/L. Moreover, we employed this method to screen for thrombin inhibitors using dabigatran as an exemplary direct thrombin inhibitor (DTI), even at concentrations as low as 1 nM. Finally, the established method was successfully used to screen thrombin inhibitors in 23 different extracts from Eupolyphaga sinensis walker . The method provided not only a sensitive, specific and high throughput assay for the detection of thrombin activity in biological samples, but also a reliable strategy for the screening of thrombin inhibitors in complex matrices.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Wenmiao Zhao,Lishi Mo,Xiaoyuan Shi,Bo Liu,Guangmin Yang,Ting Deng
CoTe 2 as a potential electrode material for sodium-ion batteries (SIBs) suffers severe volume expansion and rapid capacity degradation. Herein, we propose an anionic chemistry regulation strategy to enhance the redox performance of CoTe 2 by Se substitution which creates more porosity inside the structure to accommodate the volume expansion. Physical characterizations and theoretical calculations have studied the structural and dynamical influence of Se/Te ratio on the overall performance. As a result, CoSe 1.33 Te 0.67 @C with a 〈1 1 1〉 preferential growth direction exhibited the most balanced performance, which delivered a high initial specific capacity of 795 mAh/g at 0.05 A/g, a high rate performance of 5 A/g and a capacity retention of 370 mAh/g after 400 cycles at 0.5 A/g. Accordingly, a full SIB was constructed with a Na 3 V 2 (PO 4 ) 3 cathode and a CoSe 0.67 Te 0.33 @C anode, delivering a maximum energy density of 197.2 Wh kg −1 . These results have proved that anionic chemistry regulation is quite efficient to synthesize metal tellurides with high redox activity and long life-span for SIB .
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JOURNAL OF FLUORESCENCE
·
2015
·Vol. 25
·期 5
·1397-1402
·
IF 1.927
Cao Yujuan,Wei Jiongling,Wu Wei,Wang Song,Hu Xiaogang,Yu Ying
In the present work, the CdTe quantum dots were covalently conjugated with permethylated- β -cyclodextrin (OMe- β -CD) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as cross-linking reagent. The obtained functional quantum dots (OMe- β -CD/QDs) showed highly luminescent, water solubility and photostability as well as good inclusion ability to malachite green. A sensitive fluorescence method was developed for the analysis of malachite green in different samples. The good linearity was 2.0 × 10 −7 –1.0 × 10 −5 mol/L and the limit of detect was 1.7 × 10 −8 mol/L. The recoveries for three environmental water samples were 92.0–108.2 % with relative standard deviation (RSD) of 0.24–1.87 %, while the recovery for the fish sample was 94.3 % with RSD of 1.04 %. The results showed that the present method was sensitive and convenient to determine malachite green in complex samples.
The analytical mechanism of OMe-β-CD/QDs and its linear response to MG
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JOURNAL OF CONTROLLED RELEASE
·
2015
·
IF 7.705
HongYan Zhu,ShengYu Zhang,Yong Ling,GuoLiang Meng,Yu Yang,Wei Zhang
Hypoxia is a characteristic of cancer and plays a key role in tumorigenesis, angiogenesis and resistance to cancer therapies. SiRNA treatment is effective against hypoxic tumors by gene silencing. However, siRNA delivery to the hypoxic regions of solid tumors still presents a challenge due to the distance from blood vessels and the increased presence of efflux transporters. Therefore, tumor therapies would be improved through the immediate development of an effective siRNA delivery system to hypoxic regions. To this end, we synthesized a system to deliver HIF-1α siRNA into hypoxic tumor cells. The system consists of a functional shell composed of 2-deoxyglucose (DG)-polyethylene glycol (PEG) connected with the compound of lipoic acid , lysine and 9-poly- d -arginine (LA–Lys–9R) by a hydrazone bond and a core of CdTe quantum dots (QDs). The molecular structure of DG–PEG–LA–Lys–9R was confirmed by liquid chromatography–mass spectrometry (LC–MS), nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared spectroscopy (FTIR), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The multifunctional CdTe QDs measured approximately 200 nm and showed excellent biocompatibility , perfect siRNA binding capability and enhanced hypoxic tumor targeting. Importantly, the system described here is pH-responsive with a hydrazone bond; therefore, it avoids GLUT1 receptor-mediated endocytic recycling, resulting in irreversible delivery of the siRNA. We used Western blots to confirm the superior gene silencing efficiency induced by the DG–PEG–LA–Lys–9R with hydrazone modified CdTe QDs. Here, we demonstrate high efficacy of the siRNA tumor delivery system using in vitro and in vivo experiments. In addition, these studies demonstrate that pH-responsive hybrid quantum dots show improved antitumor efficacy with decreased organ toxicity , indicating a promising siRNA delivery system for hypoxic cancer therapy.
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ACS Nano
·
2024
·
IF 15.8
Fengru Zheng,Qi Huang,Juan Xiang,Zhiwen Zhu,Jiayi Lu,Jinyang Xu,Zhaofeng Liang,Lei Xie,Fei Song,Qiang Sun
On-surface molecular self-assembly presents an important approach to the development of low-dimensional functional nanostructures and nanomaterials. Traditional strategies primarily exploit hydrogen bonding or metal coordination, yet the potential of chalcogen bonding (ChB) for on-surface self-assemblies remains underexplored. Here, we explore fabricating molecular networks via tellurium (Te)-directed chalcogen-organic interactions. Employing carbonitrile molecules as molecular building blocks, we have achieved extended 2D networks exhibiting a 4-fold binding motif on Au(111), marking a notable difference from the conventional coordinative interaction involving transition metals. Our findings, supported by density functional theory (DFT) and scanning tunneling spectroscopy (STS), show that the Te-carbonitrile interaction exhibits lower stability compared to the metal–organic coordination, and the construction of the Te-directed molecular networks does not alter the electronic properties of the involved molecules. Introducing chalcogen-directed interactions may expand the spectrum of strategies in supramolecular assembly, contributing to the design of advanced molecular architectures for nanotechnological applications.
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Zhiping Zhou,Haiqin Ying,Yanyan Liu,Wanzhen Xu,Yanfei Yang,Yu Luan,Yi Lu,Tianshu Liu,Shui Yu,Wenming Yang
This paper demonstrates a facile method to synthesize surface molecular imprinting polymer (MIP) on SiO 2 -coated CdTe QDs for selective detection of sulfadimidine (SM 2 ). The fluorescent MIP sensor was prepared using cadmium telluride quantum dots (CdTe QDs) as the material of fluorescent signal readout, sulfadimidine as template molecule, 3-aminopropyltriethoxysilane (APTES) as functional monomer and tetraethyloxysilane (TEOS) as cross-linking agent. The CdTe cores were embed in the silicon shells by a sol-gel reaction and then the molecular imprinting layers were immobilized on the surface of the SiO 2 -coated CdTe QDs. Under the optimized conditions, the relative fluorescent intensity weakened in a linear way with the increasing concentration of sulfadimidine in the range of 10–60 μmol L −1 . The practical application of the fluorescent MIP sensor was evaluated by means of analyzing sulfadimidine in the real milk samples. The recoveries were at the range of 90.3–99.6% and the relative standard deviation (RSD) ranged from 1.9 to 3.1%, which indicates the successful synthesis of the fluorescent MIP sensor. This sensor provides an alternative solution for selective determination of sulfadimidine from real milk samples.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3189-3196
·
IF 4.58
Wang Bei,Chen Miao-miao,Zhang Hai-qing,Wen Wei,Zhang Xiu-hua,Wang Sheng-fu
A fluorescence assay is described for the fluorometric determination of dopamine (DA). It based on the use of silica-coated CdTe quantum dots (QD@SiO 2 ). These were fabricated through a hydrothermal process. When DA is added to a solution of the QD@SiO 2 and then oxidized by oxygen under catalytic action of tyrosinase to form dopamine quinone, the fluorescence of QD@SiO 2 (acquired at excitation/emission wavelengths of 310/525 nm) decreases due to an electron transfer quenching processes. The assay has a linear calibration plot in the 0.05 to 30 μM DA concentration range and a 12.5 nM detection limit (at an S/N ratio of 3). The method was applied to the determination of DA in spiked human serum samples.
Schematic presentation of a fluorometric dopamine (DA) assay by using silica-coated CdTe QDs (QD@SiO 2 ). DA is oxidized by oxygen under catalytic action of tyrosinase to form dopamine quinone, and this causes the quenching of fluorescence of QD@SiO 2 at excitation/emission wavelengths of 310/525 nm.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2017
·
IF 3.133
Xiao Zhang,Yiming Zhou,Yanling Pei,Yuexing Chen,Bifei Yuan,Shuangmei Zhang,Yuan Deng,Shengkai Gong,Jiaqing He,Li-Dong Zhao
SnTe, one lead-free analogue of PbTe, has been paid attention in thermoelectric community because it has the similar rock-salt crystal structure and electronic band structure . However, SnTe possesses a very high total thermal conductivity (∼10.0 W m −1 K −1 at room temperature) and a mediocre ZT (∼0.2 at 723 K). In this study, to reduce its thermal conductivity, we prepare SnTe samples by melting reaction followed by mechanical alloying and spark plasma sintering . We demonstrate that both total thermal conductivity and lattice thermal conductivity can be considerably reduced through reducing particle size from micro-scale to nano-scale via facially mechanical alloying method. Experimental results show that the total thermal conductivity at room temperature can be reduced from ∼10 W m −1 K −1 for SnTe ingot to ∼3Wm −1 K −1 for SnTe with mechanical alloying 25 h. Correspondingly, the lattice thermal conductivity at room temperature was reduced from ∼5Wm −1 K −1 to ∼2Wm −1 K −1 . Combination of reduced thermal conductivity and moderate power factor, average ZT ave can be increased by 33.3%, from 0.09 for SnTe ingot to 0.12 for SnTe with nano-grain size.
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NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 4
·1736-1743
·
IF 3.269
Yongling Ding,Hong Yin,Shirley Shen,Kangning Sun,Futian Liu
A chitosan-based drug delivery system, composed of Fe3O4 magnetic nanoparticles (MNPs), CdTe@ZnS quantum dots (QDs) and the anti-cancer drug doxorubicin (DOX), was designed and fabricated. The surfaces of MNPs were modified with carboxymethyl chitosan (CMCS) to minimise the possible fluorescence quenching triggered by magnetic cores. Glutaraldehyde was used as a crosslinking agent to create chemical covalent conjunction between different components, thus enhancing the stability of the obtained nanocomposites. The resulting nanocomposites retain favourable magnetic and fluorescence properties and can be used for bimodal cellular imaging. A sustained pH dependent DOX release with more release at pH = 5.3 was observed. The nanocomposites exhibited low cytotoxicity and CLSM images confirmed the cell fluorescence labelling properties and time-dependent cellular uptake. In summary, the chitosan-based nanocomposites developed in this work provided highly integrated functionalities and are promising for controlled drug delivery and also suitable for in situ cellular imaging.
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TALANTA
·
2017
·
IF 4.162
Sifang Lv,Feng Chen,Chunyan Chen,Xiaoming Chen,Hang Gong,Changqun Cai
We report a rapid and facile resonance light scattering (RLS) technique that utilizes CdTe quantum dots (CdTe QDs) probe to detect microRNA-122. The RLS sensor is ingeniously designed with P1 and P2 , two cDNA sequence probes with partially complementary sequences to miRNA-122. The amine-modified P1 and P2 are coupled to the surface of QDs to form functional QDs-P1 and QDs-P2 conjugates, which are collectively referred to as QDs-P. The cDNAs hybridize with the target miRNA to rapidly induce the self-assembly of QDs probe and change RLS intensity. The proposed technique can detect miRNA-122 within 40 min. RLS intensity is enhanced in proportion with miRNA-122 concentrations of 0.16–4.80 nM and has a low detection limit of 9.4 pM. In addition, the assay satisfactorily detects miRNAs in human serum samples. Thus, the assay has considerable potential for the analysis of other interesting tumor makers.
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JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2017
·Vol. 100
·期 12
·5723-5730
·
IF 2.841
Zhiwei Zhou,Junyou Yang,Qinghui Jiang,Dan Zhang,Jiwu Xin,Xin Li,Yangyang Ren,Xu He
In this work, the thermoelectric properties of SnTe have been regulated synergistically by introduction of n -type ZnO nanoinclusions. On one hand, the excessive holes in SnTe matrix were compensated properly by n -type ZnO, and the Seebeck coefficient has been improved greatly due to the carrier energy filtering effect by a large quantity of ZnO-SnTe hetero-junction interface barriers; On the other hand, the thermal conductivity gets reduced by the enhanced phonon scattering from multiscale hierarchical architectures. Consequently, a maximum ZT of ~0.9 at 873K has been obtained in the 0.8 wt% sample, which enhances by 112% in comparison with the nanoinclusion-free sample.
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BIOMATERIALS
·
2017
·
IF 8.402
Feng Li,Tianyu Li,Wei Cao,Lu Wang,Huaping Xu
Cisplatin (CDDP) has received worldwide approval for clinical use in the past decades. However, its development in cancer chemotherapy was overshadowed by severe side effects and drug resistance. Herein, we developed a CDDP drug delivery system with high encapsulation efficiency and near-infrared light stimuli-responsive drug release properties based on the coordination of novel tellurium-containing block polymer (PEG-PUTe-PEG) and CDDP. The nanocarriers made from PEG-PUTe-PEG were loaded with CDDP and indocyanine green (ICG) simultaneously. The coordination chemistry between CDDP and tellurium guaranteed the nanocarrier a high stability in plasma and prolonged circulation time in vivo by reducing possible penetration of water molecule into the nanoparticles . Under the stimuli of a near-infrared laser, an amount of ROS can be generated by irradiation of ICG. The tellurium is easily oxidized by ROS because of the low electronegativity of tellurium. The CDDP could be rapidly released from the nanocarriers along with the oxidation of the tellurium at the tumor sites as the oxidized tellurium will weaken the coordination interaction with CDDP. In addition, the encapsulated ICG played a synergistic antitumor effect through photothermal effect with mild laser irradiation . The integrated strategy achieved higher antitumor efficacy and showed minimal side effects compared with the CDDP alone.
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