TALANTA
·
2018
·
IF 4.244
Xiao Sun,Jing Li,Li Xu
In the present study, the narrow-dispersed penetrable poly(methacrylic acid- co -ethylene glycol dimethacrylate) (poly(MAA- co -EDMA)) microspheres were successfully synthesized based on the sacrificial support method. The poly(MAA- co -EDMA) microspheres mirrored the porous structure of the sacrificial support, i.e. penetrable silica, characteristic of copious mesopores and throughpores. In addition, they possessed large surface area, adjustable hydrophobicity and the cation-exchange ability. Owing to their multi functionalities, they were applied as chromatographic stationary phase to separate proteins in different separation modes, including reversed phase, hydrophobic interaction and weak cation exchange. Moreover, thanks to their throughpores, fast separation at low column backpressure could be achieved in these three modes. Both protein recovery and column stability were satisfactory. The penetrable poly(MAA- co -EDMA) microspheres were potential stationary phase matrix for fast protein separation.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Ning Li,Di Wu,Na Hu,Guangsen Fan,Xiuting Li,Jing Sun,Xuefeng Chen,Yourui Suo,Guoliang Li,Yongning Wu
The present study reported a facile, sensitive, and efficient method for enrichment and determination of trace polycyclic aromatic hydrocarbons (PAHs) in food samples by employing new core–shell nanostructure magnetic covalent organic framework hybrid microspheres (Fe3O4@COF-(TpBD)) as the sorbent followed by HPLC-DAD. Under mild synthetic conditions, the Fe3O4@COF-(TpBD) were prepared with the retention of colloidal nanosize, larger specific surface area, higher porosity, uniform morphology, and supermagnetism. The as-prepared materials showed an excellent adsorption ability for PAHs, and the enrichment efficiency of the Fe3O4@COF-(TpBD) could reach 99.95%. The obtained materials also had fast adsorption kinetics and realized adsorption equilibrium within 12 min. The eluent was further analyzed by HPLC-DAD, and good linearity was observed in the range of 1–100 ng/mL with the linear correlation being above 0.9990. The limits of detection (S/N = 3) and limits of quantitation (S/N = 10) for 15 PAHs were in the range of 0.83–11.7 ng/L and 2.76–39.0 ng/L, respectively. For the application, the obtained materials were employed for the enrichment of trace PAHs in food samples and exhibited superior enrichment capacity and excellent applicability.
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ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Shan Liu,Maochao Wei,Xiangli Zheng,Shuai Xu,Fangquan Xia,Changli Zhou
In the paper, a novel and sensitive electrochemical sensor based on modification of electroactive alizarin red S functionalized mesoporous silica material SBA15 onto glassy carbon electrode (ARS-SBA15/GCE) was developed. Alizarin red S , called electrochemical probe that can selectively recognize polycyclic aromatic hydrocarbons (PAHs), as tools for the detection of tricyclic aromatic hydrocarbon anthracene. The morphology and interface property of ARS modified SBA15 (ARS-SBA15) were examined by transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR). Taking advantage of the π-π stacking force between alizarin red S and anthracene, the ARS-SBA15/GCE sensor could detect anthracene quantitatively in a wide range of 1.0 pM–10.0 nM and a low detection limit of 0.5 pM (S/N = 3). Other PAHs, such as naphthalene, phenanthrene, pyrene, and benzo[a]pyrene show little interference on the detection. Consequently, a simple and sensitive electrochemical method was proposed for the determination of anthracene, which can be used to determine anthracene in waste water samples. The electrochemical method provides a general tool that complements the commonly used spectroscopic methods and immune method for the detection of PAHs.
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Biomaterials Science
·
2014
·Vol. 3
·期 3
·490-499
·
IF 0
Gongyan Liu,Quanqing Luo,Haiqi Gao,Yuan Chen,Xing Wei,Hong Dai,Zongcai Zhang,Jian Ji
In cancer therapy, surface engineering of drug delivery systems plays an essential role in their colloidal stability, biocompatibility and prolonged blood circulation. Inspired by the cell membrane consisting of phospholipids and glycolipids, a zwitterionic phosphorylcholine functionalized chitosan oligosaccharide (PC-CSO) was first synthesized to mimic the hydrophilic head groups of those amphipathic lipids. Then hydrophobic stearic acid (SA) similar to lipid fatty acids was grafted onto PC-CSO to form amphiphilic PC-CSO-SA copolymers. Cell membrane-mimetic micelles with a zwitterionic surface and a hydrophobic SA core were prepared by the self-assembly of PC-CSO-SA copolymers, showing excellent stability under extreme conditions including protein containing media, high salt content or a wide pH range. Doxorubicin (DOX) was successfully entrapped into polymeric micelles through the hydrophobic interaction between DOX and SA segments. After fast internalization by cancer cells, sustained drug release from micelles to the cytoplasm and nucleus was achieved. This result suggests that these biomimetic polymeric micelles may be promising drug delivery systems in cancer therapy.
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JOURNAL OF MEMBRANE SCIENCE
·
2013
·
IF 4.093
Zhiming Yang,Zhaogen Wang,Xueping Yao,Xiaoqiang Chen,Yong Wang
Fluorescent polymeric spheres with uniform sizes are of great interests in the measurement and calibration of the pore sizes and defects of porous media, including separating membranes. We reported the preparation of such nanospheres by dissolving amphiphilic block copolymers of polystyrene- block -poly(2-vinyl pyridine) (PS- b -P2VP) together with chromophores (perylene and pyrene) in acetic acid at elevated temperatures. Micellar nanosphere with the chromophores residing in the cores were formed through the heating-enabled micellization process. Following quaternization of the P2VP coronae gave the spheres a positively charged surface and a good water dispersibility. Scanning and transmission electron microscopy and dynamic light scattering analysis confirmed that the micellar nanospheres were uniform in size both in the dry and wet state. The micelle size and the thickness of coronae can be tuned by altering the molecular weight of the constituent blocks. Encapsulation of chromophores in the micelle cores provided a strong fluorescent emission to the micellar nanospheres, while quaternization of the P2VP coronae gave the nanospheres a greatly variable size according to pHs and salt concentrations in the surroundings. Taking advantages of their uniform size, water dispersibility, and fluorescent emission for accurate detection, we demonstrated that the micellar nanospheres were capable of characterizing pore sizes of separating membranes and detecting their large-pore defects.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2014
·Vol. 17
·期 33
·21158-21163
·
IF 4.198
Meng Jiang,Zhijiang Qian,Xufeng Zhou,Xing Xin,Jinghua Wu,Chao Chen,Gongjun Zhang,Gaojie Xu,Yuchuan Cheng
A structure of hexadecyl trimethyl ammonium bromide (CTAB) micelle-assisted reduced graphene oxide–Ag nanoparticle (rGO–AgNP) hybrids is designed and fabricated for SERS detection of nonpolar polycyclic aromatic hydrocarbons (PAHs), in which CTAB micelles act as the host material to capture PAH molecules. This method provides stable aqueous suspensions of functionalized graphene with an alkyl chain, since the rGO–AgNP hybrids do not need to be pre-modified by CTAB. The result shows that the CTAB-assisted rGO–AgNP substrate has excellent SERS performance toward PAHs and ideal stability under continuous laser radiation. With further optimization, the detection limits of pyrene and perylene were 10−6 M and 10−7 M, respectively. Two different PAH molecules could be detected simultaneously by their characteristic peaks.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2014
·
IF 4.245
Zheng Wang,Chao Chen,Qi Zhang,Min Gao,Ju Zhang,Deling Kong,Yanjun Zhao
To precisely manipulate the intracellular delivery of pleiotropic curcumin , this work reports two types of acid-responsive polymer–curcumin conjugates with different structures. One or two amphiphilic poly(ethylene glycol)-co-poly(lactic acid) (PEG-PLA) copolymer(s) were linked to curcumin via pH-liable hydrazone, producing a linear or phospholipid-like conjugate, respectively. Both conjugates efficiently self-assembled into micellar nanocarriers with enhanced stability in contrast to the paralleling PEG-PLA micelles. In comparison to the micelles assembled by phospholipid-like conjugates, the linear conjugate micelles exhibited similar size, doubled loading dose, higher release rate, and hence enhanced cellular uptake and cytotoxicity at the cost of increased critical micelle concentration. This work highlighted the importance of precise tuning of polymer–drug conjugate architecture in determining the pharmaceutical properties and thus delivery efficiency of the corresponding conjugate micelles.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2014
·
IF 3.785
Yuliang Xiao,Pingli Li,Yanna Cheng,Xinke Zhang,Juzheng Sheng,Decai Wang,Juan Li,Qian Zhang,Chuanqing Zhong,Rui Cao,Fengshan Wang
The purpose of this report was to demonstrate the effect of amphiphilic polysaccharides-based self-assembling micelles on enhancing the oral absorption of low molecular weight chondroitin sulfate (LMCS) in vitro and in vivo , and identify the transepithelial transport mechanism of LMCS micelles across the intestinal barrier. α-Linolenic acid-low molecular weight chondroitin sulfate polymers(α-LNA–LMCS) were successfully synthesized, and characterized by FTIR, 1 HNMR, TGA/DSC, TEM, laser light scattering and zeta potential . The significant oral absorption enhancement and elimination half-life ( t 1/2 ) extension of LNA–LMCS2 in rats were evidenced by intragastric administration in comparison with CS and LMCS. Caco-2 transport studies demonstrated that the apparent permeability coefficient ( P app ) of LNA–LMCS2 was significantly higher than that of CS and LMCS ( p < 0.001), and no significant effects on the overall integrity of the monolayer were observed during the transport process. In addition, α-LNA–LMCS micelles accumulated around the cell membrane and intercellular space observed by confocal laser scanning microscope (CLSM). Furthermore, evident alterations in the F-actin cytoskeleton were detected by CLSM observation following the treatment of the cell monolayers with α-LNA–LMCS micelles, which further certified the capacity of α-LNA–LMCS micelles to open the intercellular tight junctions rather than disrupt the overall integrity of the monolayer. Therefore, LNA–LMCS2 with low cytotoxicity and high bioavailability might be a promising substitute for CS in clinical use, such as treating osteoarthritis , atherosclerosis , etc.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Hailong Peng,Wenjian Li,Fangjian Ning,Lihua Yao,Mei Luo,Xuemei Zhu,Qiang Zhao,Hua Xiong
A novel amphiphilic chitosan derivative of N,N-dimethylhexadecyl carboxymethyl chitosan (DCMCs) was synthesized. The structure of DCMCs was confirmed via FT-IR and 1H NMR, and the critical micelle concentration (CMC) was investigated by fluorescence spectroscopy. The results indicated that DCMCs has hydrophilic carboxyl and hydrophobic methylene groups and the CMC value was 23.00 mg·L–1. The polymeric liposomes (DCMCs/cholesterol liposomes, DC-Ls) were developed, and its properties were evaluated. The DC-Ls exhibited multilamellar spheres with positive charge (+73.30 mV), narrow size distribution (PDI = 0.277), and good crystal properties. Salidroside was first to encapsulate into DC-Ls. Compared with traditional liposomes (phosphatidylcholine/cholesterol liposome, PC-Ls), DC-Ls showed higher encapsulation efficiency (82.46%) and slower sustained release rate. The in vitro salidroside release from DC-Ls was governed by two distinct stages (i.e., burst release and sustained release) and was dependent on the pH of the release medium. The case II transport and case I Fichian diffusion were the main release mechanisms for the entire release procedure. These results indicated that DC-Ls may be a potential carrier system for the production of functional foods that contain salidroside or other bioactive food ingredients.
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CARBOHYDRATE POLYMERS
·
2013
·
IF 3.479
Yinhui Li,Shudong Lin,Jiwen Hu,Guojun Liu,Gangwei Zhang,Yuanyuan Tu,Hongsheng Luo,Wei Li
Cu 2+ /amylose assemblies of various sizes were prepared through the Cu 2+ ion induced-assembly of amylose. These assembly structures were characterized via transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), dynamic light scattering (DLS), 1 H NMR analysis, fluorescence spectroscopy (FL) and UV–vis absorption spectroscopy (UV–vis). The results from these characterizations revealed the existence of a complexation effect and/or a bridging effect between the hydroxyl groups of amylose and Cu 2+ ions, and that the formation of the hydrophobic domains promoted the formation of Cu 2+ /amylose assemblies. The use of other metal ions to induce the formation of spherical, flower- and wire-like amylose assemblies was investigated as well. A preliminary investigation on the ability of amylose to capture various metal ions was also performed, and the results of this work demonstrated that amylose could bind quantitatively metal ions that were at low concentrations. This work provided an alternative strategy for the recovery of precious metals from metal ion-containing aqueous solutions and the reduction of water pollution.
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RSC Advances
·
2015
·Vol. 5
·期 112
·92292-92302
·
IF 3.84
Meng Cai,Mengyuan Ye,Xingxing Shang,Honghao Sun,Mingxing Liu,Hongmei Sun,Zhuo Ma,Hongda Zhu
Polymeric micelles as nanocarrier-based drug delivery systems provide an innovative platform for selectively delivering active molecules and offer better antitumour activity. However, circulation stability in vivo, controllable drug intracellular release and high targeting efficiency are several practical challenges for micelles. Therefore, we developed cRGD-modified and shell crosslinked micelles (RSCMs) based on amphiphilic poly(styrene-co-maleic anhydride) (SMA). RSCMs exhibited a spherical shape and were homogeneous with an average diameter of 106.1 nm and a low polydispersity of 0.087. In comparison, after crosslinking the micelles possessed better stability against dilution and stronger redox-response towards dithiothreitol (DTT). The SMA micelles displayed high drug loading capacity for hydrophobic DOX with a drug loading of approximately 14.1–19.2% (w/w) and an encapsulation efficiency of 72.1–82.7% (w/w). The in vitro release studies of shell crosslinked micelles showed that DOX release was minimal (<25%, 24 h) under physiological conditions. However, in the presence of 10 mM DTT, accelerated release of DOX was achieved (60%, 24 h). MTT assays in B16F10 cells indicated that RSCMs displayed low cytotoxicity up to a concentration of 500 μg ml−1. Moreover, the IC50 value showed that cRGD-DOX-ss-M could be more effective than other groups. In vitro, cellular uptake was further studied with confocal laser scanning microscopy and flow cytometry, both the qualitative and quantitative results demonstrated that cRGD-DOX-ss-M possessed much better specificity to cancer cells and superior stimulated release property in cytoplasm. Notably, cRGD-DOX-ss-M could more efficiently be delivered to and release into the nuclei of αvβ3 integrin over expressing tumor cell line (B16F10) than counterparts of integrin-deficient tumor cells (Hela). Thus, these cRGD-modified redox-sensitive micelles have appeared as a technology platform with great potential for targeted anticancer drug delivery and release for integrin over expressing tumor cells.
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Materials
·
2015
·Vol. 8
·期 2
·379-391
·
IF 2.651
Yanchao Liang,Zhihui Su,Yao Yao,Na Zhang
A novel polymer-drug conjugate was prepared by the chemical reaction between the copolymer Pluronic P123 and the docetaxel via a pH sensitive hydrazone bond. These pluronic P123-docetaxel (DTX) conjugates (P123-DTX) could form the stable drug-loaded materials that can self-assemble into the defined nano-micelles in aqueous solution because of their obvious amphiphilic property and low critical micelle concentration. The spherical morphology and particle size of the prepared nano-micelles were characterized by transmission electron microscopy and dynamic light scattering, respectively. Moreover, after the introduction of pH sensitive hydrazone bond, P123-DTX micelle showed a pH dependent drug release behavior. At pH 5.0 (in 48 h), the cumulative release amount of DTX were ~84.9%, which is about six times higher than that at pH 7.4. The prepared novel p123-DTX conjugates may offer a great benefit for drug delivery and controlling the drug release.Keywords:docetaxel;conjugates;nano-micelles;stability;pH controlled release
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