CARBOHYDRATE RESEARCH
·
2024
·
IF 2.4
Lin Wang,Yu Pang,Zhongwen Su,Meihua Xin,Mingchun Li,Yangfan Mao
N -(sodium 2-hydroxypropylsulfonate) chitosan (HSCS), N -sulfonate chitosan (SCS) and N -isonicotinic sulfonate chitosan (ISCS) were prepared. The structures of the prepared chitosan derivatives were characterized by nuclear magnetic resonance ( 1 H NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, ultraviolet–visible (UV–vis) spectroscopy and elemental analysis (EA). Antibacterial and antibiofilm activities of these chitosan derivatives were evaluated in vitro . The minimum inhibitory concentration (MIC) of HSCS and SCS against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) were 0.625 mg/mL and 0.156 mg/mL, respectively. ISCS exhibited MIC values of 0.313 mg/mL and 0.078 mg/mL against E. coli and S. aureus , respectively. ISCS demonstrated superior antibacterial and antibiofilm properties compared to SCS and HSCS. These findings suggest that the incorporation of a pyridine structure into sulfonate chitosan enhances its antibacterial and antibiofilm activities, and the prepared ISCS has a promising application prospect for controlling the reproduction of microorganisms in the field of food packaging.
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Biology-Basel
·
2025
·Vol. 14
·期 3
·226
·
IF 3.5
Zonghan Jiang,Leisheng Sun,Yuanyuan Li,Haoyu Li,Yu Fu,Jiyun Li,Zhiliang Sun
Simple SummaryEscherichia coliis a ubiquitous pathogen, with certain strains causing severe diarrhea and intestinal illnesses, which pose significant health risks to both children and animals. As antimicrobial resistance continues to rise, traditional antibiotics are increasingly ineffective, highlighting the urgent need for novel therapeutic strategies. In this study, we investigated the antimicrobial peptide D-CONGA-Q7, aiming to evaluate its efficacy and underlying mechanism against drug-resistantE. colistrains. Our results demonstrate that D-CONGA-Q7 effectively disrupts bacterial cell membranes, leading to bacterial death, and exhibits comparable activity against polymyxin- and meropenem-resistant strains. In vivo experiments revealed that D-CONGA-Q7 significantly alleviated intestinal inflammation, reduced inflammatory cytokine levels, and promoted the repair of damaged intestinal tissues. Furthermore, D-CONGA-Q7 displayed good stability. These findings suggest that D-CONGA-Q7 not only represents a promising new approach to combat drug-resistant bacterial infections but also holds potential for clinical application.Escherichia coli(E. coli) is a zoonotic bacterium widespread in the environment, highly transmissible, and responsible for significant economic losses and millions of cases of illness annually. The rise of multidrug-resistant (MDR) strains has rendered last-line antibiotics such as polymyxin and meropenem ineffective, making the development of new antibiotics urgent. Although D-CONGA-Q7 has broad-spectrum bactericidal activity, its underlying mechanism remains poorly understood. In this study, we used in vitro and in vivo experiments to demonstrate that D-CONGA-Q7 effectively kills both antibiotic-sensitive and multidrug-resistant strains ofE. coli. D-CONGA-Q7 disrupts the cell membranes of Gram-negative bacteria, and the treatment ofE. colistrainLN175with D-CONGA-Q7 resulted in a significant up-regulation of theMlacgene, suggesting that D-CONGA-Q7 may interact with phospholipids in the cell membrane. Furthermore, in treatingK88-induced bacterial enteritis in the small intestine, D-CONGA-Q7 significantly reduced intestinal inflammation. In conclusion, this study provides a novel approach to combat drug-resistantE. coli.
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Frontiers in Chemistry
·
2025
·
IF 4.2
Pan-Pan Zhang,Xin-Jing Gui,Xue-Hua Fan,Han-Li,Hai-Yang Li,Xiao-Peng Li,Feng-Yu Dong,Yan-Li Wang,Jing-Yao,Jun-Han Shi,Rui-Xin Liu
Amomi fructus (AF) has been used for both medicinal and food purposes for centuries. However, issues such as source mixing, substandard quality, and product adulteration often affect its efficacy. This study used E-nose (EN) and headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) to determine and analyze the volatile organic compounds (VOCs) in AF and its counterfeit products. A total of 111 VOCs were detected by HS-GC-IMS, with 101 tentatively identified. Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) identified 47 VOCs as differential markers for distinguishing authentic AF from counterfeits (VIP value >1 and P < 0.05). Based on the E-nose sensor response value and the peak volumes of the 111 VOCs, the unguided Principal Component Analysis (PCA), guided Principal Component Analysis-Discriminant Analysis (PCA-DA), and Partial Least Squares-Discriminant Analysis (PLS-DA) models were established to differentiate AF by authenticity, origin, and provenance. The authenticity identification model achieved 100.00% accuracy after PCA analysis, while the origin identification model and the provenance identification model were 95.65% (HS-GC-IMS: PLS-DA) and 98.18% (HS-GC-IMS: PCA-DA/PLS-DA), respectively. Further data-level fusion of E-nose and HS-GC-IMS significantly improved the accuracy of the origin identification model to 97.96% (PLS-DA), outperforming single-source data modeling. In conclusion, the intelligent data fusion algorithm based on E-nose and HS-GC-IMS data effectively identifies the authenticity, origin, and provenance of AF, providing a rapid and accurate method for quality evaluation.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Salah Naji Sufyan,Yusof A. Y. A. Mohammed,Chenglong Mu,Kashan Bashir,Ning Wen,Muhammad Mamoor,Xiuling Jiao,Dairong Chen,Ting Wang
Flexible piezoresistive pressure sensors are crucial for next-generation wearable electronics, yet achieving simultaneous high sensitivity, durability, and mechanical robustness remains a challenge. Herein, we report a high-performance piezoresistive sensor fabricated through the integration of two-dimensional MXene nanosheets with a hierarchically structured modified polyamide 6 nanofiber net (D-PA6 NFN) using scalable vacuum filtration. Benefiting from the synergistic combination of MXene’s excellent electrical properties, web-like architecture, the composite nanofiber tensile strength (52 MPa), and flexibility (40%), the resulting sensor exhibited good sensitivity (0.189 kPa–1), a rapid response/recovery time (14/13 ms), and an ultralow detection limit (4.2 Pa). Moreover, the device maintained stability over 12,000 compression cycles. The sensor precisely captured both subtle physiological signals (pulse waveforms) and large-scale dynamic motions (joint flexion). This work establishes a scalable strategy for engineering durable, high-sensitivity electronic skins, with immediate applications in advanced human-machine interfaces and real-time physiological monitoring.
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JOURNAL OF FOOD ENGINEERING
·
2025
·
IF 5.8
Chunying Jiang,Yexuan Yao,Dan Liu,Chenlu Ma,Zhiyi Zhang,Jian Wu,Wei Liu,Ting Luo,Liqiang Zou
A cyclodextrin-liposome system (CL) was developed to encapsulate dihydromyricetin (DMY), to improve its water solubility, bioavailability, and to mask its bitter taste, thus expanding its application in the food field. The dihydromyricetin-cyclodextrin-liposome (DCL) prepared by film dispersion method showed a multivesicular structure with an average particle size of 86.77 nm and an encapsulation rate of 58.4 %. The stability analyzer showed that the DCL had excellent physical stability (instability index: 0.023 ± 0.003) and exhibited good slow-release properties. QCM-D analysis of DCL effectively inhibited the binding of DMY and mucin. The electronic tongue indicated that DCL significantly masked the bitterness of DMY. In vivo pharmacokinetic studies showed that the AUC 0–90min and Cmax of DMY delivered by DCL significantly increased by 5.83-fold and 3.38-fold, respectively, compared with the control group. This study provides a proof-of-concept for the development of an efficient DMY delivery strategy, which is expected to greatly contribute to its potential application in the field of functional foods.
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Science Advances
·
2025
·Vol. 11
·期 25
·
IF 12.5
Yang Gao,Daming Liu,Jing Zhou,Wen Zhang,Danqi Sun,Shuyang Wang,Yipeng Yang,Yuchen Xiang,Yang Zhang,Wei Lei,Tongqing Lu
Expanded polytetrafluoroethylene (e-PTFE) is extensively used in medical implants for its excellent bioinertness. Existing methods to fix e-PTFE implants on host tissues mainly use invasive anchorage such as sutures, spiral tacks, or metal skeletons, which has limitations such as being time-consuming and causing leakage and tissue damage. To overcome these limitations, we introduce a bioadhesive interface to realize the adhering fixation of e-PTFE implants. We integrate a hydrophilic and bioadhesive hydrogel layer on the hydrophobic and bioinert e-PTFE by designing a facile approach of freezing-induced micromechanical interlocking. The integration is robust enough as pulling hydrogel out of the interlocked pores on e-PTFE requires large energy dissipation. This bioadhesive interface enables instant (operating time < 10 seconds) and secure (adhesion energy >200 joules per square meter) adhering fixation of e-PTFE implants to wet tissue. The advantages for reducing inflammatory response, fibrogenesis, and the resultant postoperative adhesion are further demonstrated in a reconstructive surgery of an abdominal wall defect in rabbits.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 23
·7805-7817
·
IF 5.56
Fu Linglong,Sun Mengning,Wen Weizhang,Dong Na,Li Defa
Abstract Small ubiquitin-like modifier (SUMO) tag is widely used to promote soluble expression of exogenous proteins, which can then be cleaved by ubiquitin-like protease 1 (Ulp1) to obtain interested protein. But the application of Ulp1 in large-scale recombinant protein production is limited by complicated purification procedures and high cost. In this study, we describe an efficient and simple method of extracellular production of Ulp1 403-621 using a leaky Escherichia coli BL21(DE3), engineered by deleting the peptidoglycan-associated outer membrane lipoprotein ( pal ) gene. Ulp1 403-621 was successfully leaked into extracellular supernatant by the BL21(DE3)-Δ pal strain after IPTG induction. The addition of 1% glycine increased the extracellular production of Ulp1 403-621 approximately four fold. Moreover, extracellular Ulp1 403-621 without purification had high activities for cleaving SUMO fusion proteins, and antimicrobial peptide pBD2 obtained after cleavage can inhibit the growth of Staphylococcus aureus . The specific activity of extracellular Ulp1 403-621 containing 1 mM EDTA and 8 mM DTT reached 2.0 × 10 6 U/L. Another commonly used protease, human rhinovirus 3C protease, was also successfully secreted by leaky E. coli strains. In conclusion, extracellular production of tool enzymes is an attractive way for producing large-scale active recombinant proteins at a lower cost for pharmaceutical, industrial, and biotechnological applications. Key points
• First report of extracellular production of Ulp1
403-621
in leaky Escherichia coli BL21(DE3) strain.
• One percent glycine addition into cultivation medium increased the extracellular production of Ulp1
403-621
approximately four fold.
• The specific activity of extracellular Ulp1
403-621
produced in this study reached 2.0 × 10
6
U/L.
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BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Yimin Li,Wenjie Yuan,Jiaoqi Gao,Chao Fan,Wenzhong Wu,Fengwu Bai
In this study, the optimum induction and reaction conditions, and fermentation process of producing Ala-Gln by E. coli Origami 2 overexpressing α-amino acid ester acyltransferase (OPA) were investigated. Besides, the Ala-Gln synthesis by OPA achieved the maximum molar yield of 94.7% and productivity of 1.89 g/L/min due to the extremely high enzyme activity. On this basis, repeated-cycle batch fermentation to produce Ala-Gln indicated that OPA could maintain high Ala-Gln yields and enzyme stabilities after several cell recycling. Consequently, the cost-efficient and environmentally friendly approach for Ala-Gln production by recycling OPA makes a great contribution to further industrial-scale applications.
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BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
·
2013
·
IF 3.389
Ting Wu,Mengying He,Xixi Zang,Ying Zhou,Tianfu Qiu,Siyi Pan,Xiaoyun Xu
Flavonoids exhibit a broad range of biological activities including antibacterial activity. However, the mechanism of their antibacterial activity has not been fully investigated. The antibacterial activity and membrane interaction of 11 flavonoids (including 2 polymethoxyflavones and 4 isoflavonoids) against Escherichia coli were examined in this study. The antibacterial capacity was determined as flavonoids > polymethoxyflavones > isoflavonoids. Using fluorescence, it was observed that the 5 flavonoids rigidified the liposomal membrane, while the polymethoxyflavones and isoflavonoids increased membrane fluidity. There was a significant positive correlation between antibacterial capacity and membrane rigidification effect of the flavonoids. A quantitative structure–activity relationship (QSAR) study demonstrated that the activity of the flavonoid compounds can be related to molecular hydrophobicity (CLogP) and charges on C atom at position3 (C3). The QSAR model could be used to predict the antibacterial activity of flavonoids which could lead to natural compounds having important use in food and medical industry.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2024
·
IF 3.9
Zhonghua Wang,Wei Song,Wanqing Wei,Hejia Qi,Weiwei Meng,Jia Liu,Xiaomin Li,Cong Gao,Liming Liu,Guipeng Hu,Yiwen Zhou,Jing Wu
Natural chondroitin sulfate (CS), extracted from animal cartilage, is widely used in the pharmaceuticals and foods. However, contamination with animal-derived heteropolysaccharides presents significant challenges, including potential immune responses. To address this, we developed a green and efficient method for synthesizing chondroitin sulfate E (CSE) via enzymatic synthesis, identifying EcCHST15, a sulfotransferase that catalyzes the conversion of chondroitin sulfate A (CSA) to CSE. We investigated the novel catalytic mechanism of CHST15 through quantum mechanical (QM) calculations and experimental validation, confirming its alignment with the SN2 reaction mechanism. Subsequently, we enhanced the catalytic efficiency of CHST15 using protein engineering, improving the catalytic efficiency from 18.1% in the wild type (WT) to 62.5% in the M7 mutant—a 3.5-fold increase. Finally, we constructed a six-enzyme cascade whole-cell catalyst, achieving a 72.2% conversion of 15 g/L CSA to produce CSE within 24 h. These findings offer a promising strategy for the industrial production of CSE.
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NANOTECHNOLOGY
·
2020
·Vol. 31
·期 39
·395602
·
IF 3.551
Hongchao Geng,Ning Jiang,Chenyi Li,Xingqi Zhu,Yan Qiao,Qingyun Cai
A novel visible light-driven photocatalyst (represented as Mn-CdS/ZCISe/CIS/TiO2) for the passivation of E. coli was prepared with TiO2 nanowires as support and using CuInS2 (CIS) and ZnCuInSe (ZCISe) quantum dots (QDs), as well as Mn-doped CdS (Mn-CdS) nanoparticles (NPs) as sensitizers. The use of CIS and ZCISe QDs and Mn-CdS NPs extends the light harvest region to visible light. The photoelectric conversion efficiency was consequently improved, with a photocurrent density of 12.5 mA cm−2, about 60 times that of pure TiO2 nanowires. The germicidal efficiency of the photocatalyst was assessed by passivation of E. coli, 96% bacteria in 50 ml 105 colony forming units (CFU) ml−1 solution were killed within 50 min. Besides the high efficiency, the composite has good stability and satisfactory recycling performance. The antibiotic mechanism was also performed by using photoluminescence and a scavenging agent of different active matter, revealing that the photo-generated holes play a major role in the sterilization process.
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Desalination and Water Treatment
·
2024
·
IF 1
Xiaoting Hong,Yumei Zhou,Zhuoliang Ye,Haifeng Zhuang,Wanpeng Liu,K.S. Hui,Zhi Zeng,Xianhuan Qiu
Ag 3 PO 4 /TiO 2 nanocomposite was fabricated by an in situ precipitation method and then blended into poly(vinylidene fluoride) (PVDF) casting solution to prepare the ultrafiltration membrane via wet phase inversion technique. The water flux and bovine serum albumin (BSA) rejection rate of membrane were investigated; meanwhile, the ultrafiltration membrane morphologies and structural properties were analyzed using scanning electron microscope (SEM) and X-ray diffraction. Compared with the control membrane, the permeate performance of blended membranes was improved while possessing a steady BSA retention due to enhanced hydrophilicity. Mechanical tests revealed that the modified membranes exhibited a larger tensile strength and breakage elongation. SEM images and the halo zone testing were employed to assess the antibacterial performances of the nanocomposite membranes against Escherichia coli. The antibacterial tests confirmed that the modified membranes showed an effective antibacterial property against E. coli.
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