APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Yu-Xuan Dai,Wen-Li Xin,Lian-Hua Xu,Ji Li,Yi-Xuan Li,Junji Li,Serge Cosnier,Xue-Ji Zhang,Robert S. Marks,Dan Shan
Rational design highly active transition metal or transition metal oxides/nanocarbon hybrids is one of the feasible way to deliver a cooperative oxygen reduction (ORR) with a synergistic effect of the hybrid interface. Herein, based on benzimidazole-5-carboxylate (BIMC), CoO/Co-N-C nanocomposites are fabricated via 2-methylimidazole (2-MeIm)-tuned “4-Self” strategy, namely, self-templating, self-adapting, self-assembly and self-catalysis. As a base competitive ligand with N and C sources, 2-MeIm modulates the dispersion of BIMC in methanol to generate collodial-like BIMC tiny particles, the coordination ability of BIMC to Co(II), the composition and morphology of the self-assembled precursor (Co-BM), and as well as the conductivity of the resultant electrocatalyst. Impressing, carbonization of Co-BM at 800 °C under N 2 atmosphere endows the pyrolysis product (Co-BM-800) with the well-dispersed Co/CoO riveted on nitrogen-doped carbon, as well as the enhanced ORR performance in terms of positive onset-potential (0.890 V vs. RHE), large diffusion limiting current density (5.21 mA cm −2 ), and high stability.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Xueyin Ru,Ning Tao,Yanyan Feng,Qingqing Li,Qingguo Wang
Enzymatic browning is one of the essential issues affecting the quality of fresh-cut produce. In this study, we identified a novel, safe, and effective anti-browning agent, 3-mercapto-2-butanol, which can prevent fresh-cut potatoes from browning for 5 d under the concentration of 25 μL L −1 at 5 °C. The inhibitory effect of 3-mercapto-2-butanol was close to sodium bisulfite. 3-Mercapto-2-butanol was further identified as a competitive inhibitor which directly inhibited the activity of polyphenol oxidase (PPO) by enzyme kinetic analysis, and the expression levels of PPO-related genes ( POT32 and POT33 ) were decreased after 3-mercapto-2-butanol treatment. Besides, the concentration of enzymatic browning substrate tyrosine (Tyr) in the potato chips was found significantly higher in 3-mercapto-2-butanol treatment group than in the control group. We speculated that 3-mercapto-2-butanol could reduce the consumption of Tyr by inhibiting the activity of PPO. Our finding not only provided a novel and safe anti-browning agent candidate (3-mercapto-2-butanol) to efficiently solve the browning issue for fresh-cut food products, but also preliminarily revealed its mechanism in browning inhibition.
研究领域:
食品科学
·
植物生理学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Tong Zhou,Qingqing Chai,Man Zhang,Heping Cui,Ahmad Mohammad,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
Exogenous xylose (Xyl) could promote 2-furfural formation but inhibit pyrazines generation during its co-heating with alanine (Ala)-Xyl Amadori rearrangement product (AX-ARP). The activation energy of AX-ARP formation from Ala and Xyl was considerably lower than that of pyrazine formation from Ala and glyoxal (GO). Accordingly, the preferential interaction of exogenous Xyl with regenerated Ala triggered a continuous cycle of consumption and regeneration of Ala in AX-ARP-Xyl model. In this way, functioning as a catalyst, regenerated Ala facilitated the continuous conversion of AX-ARP into deoxyxylosones (DXs). However, exogenous Xyl accelerated the pH drop during the thermal treatment of AX-ARP, which favored the conversion of DXs into 2-furfural while limiting the transformation of their α-dicarbonyl fragments into pyrazines. Model reactions of AX-ARP-[ 13 C 5 -Xyl] further confirmed that exogenous Xyl was progressively converted into 2-furfural, while its downstream products, such as GO and methylglyoxal (MGO), did not exhibit an enhanced tendency to form pyrazines.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Ningze Zheng,Shurui Liu,Jianheng Chen,Yue Xu,Wenyin Cao,Jinyi Lin,Guang Lu,Guigen Zhang
The development of biomolecule delivery systems is essential for the treatment of various diseases such as cancer, immunological diseases, and metabolic disorders. For the first time, we found that SARS-CoV-2-encoded nonstructural protein 2 (NSP2) can be secreted from the cells, where it is synthesized. Brefeldin A and H89, inhibitors of ER/Golgi secretion pathways, did not inhibit NSP2 secretion. NSP2 is likely secreted via an unconventional secretory pathway. Moreover, both secreted and purified NSP2 proteins were able to traverse the plasma membrane barrier and enter both immortalized human umbilical vein endothelial cells and tumor cell lines. After entry, the NSP2 protein was localized in only the cytoplasm. Cytochalasin D, a potent inhibitor of actin polymerization, inhibited the entry of NSP2. NSP2 can carry other molecules into cells. Burkholderia lethal factor 1, a monomeric toxin from the intracellular pathogen Burkholderia pseudomallei, has demonstrated antitumor activity by targeting host eukaryotic initiation translation factor 4A. An NSP2-BLF1 fusion protein was translocated across the cellular membranes of Huh7 cells and mediated cell killing. By using different approaches, including protein purification, chemical inhibition, and cell imaging, we confirm that NSP2 is able to deliver heterologous proteins into cells. NSP2 can act as a potential delivery vehicle for proteins.
研究领域:
分子生物学
·
细胞生物学
·
癌症研究
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
FUEL
·
2025
·
IF 7.5
Wanpeng Li,Xuewei Zhang,Guangmai Zhao,Tianhan Zhu,Mi Luo,Tianyu Gao,Chenguang Wang,Xiaoyu Chen,Jin Tan,Yuhe Liao,Haiyong Wang
The efficient and sustainable transformation of biomass-derived furfural into value-added chemicals is a key challenge in green chemistry. In this study, a series of Pt/CeO 2 catalysts with varying Pt loadings (0.1–2.0 wt%) were synthesized via a carbonization–reduction method and applied for the selective conversion of furfural to 2-furfural diethanol in ethanol under mild conditions. Comprehensive characterization using XRD, XPS, EPR, TEM, and Py-FTIR revealed that the 1.0 wt% Pt/CeO 2 catalyst exhibited optimal properties, including high dispersion of metallic Pt⁰, abundant oxygen vacancies, enriched Ce 3+ species, and dominant Lewis acid sites. Catalytic tests demonstrated that under mild conditions (20 °C, 4 MPa N 2 ), the 1.0 wt% Pt/CeO 2 catalyst achieved the highest yield (92.17 %) and furfural conversion (95.41 %). Mechanistic studies suggest a proposed redox-driven tandem hydrogenation–condensation pathway, where the synergistic effect between Pt⁰ sites and oxygen vacancies facilitates furfural activation, hydrogen transfer, and product formation. This work provides a mechanistic understanding of furfural upgrading over Pt/metal oxide interfaces and offers a promising strategy for the rational design of high-performance catalysts for biomass valorization.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 3
·898-909
·
IF 1.606
Xu Jian-Miao,Cheng Feng,Fu Fang-Tian,Hu Hai-Feng,Zheng Yu-Guo
L -2-aminobutyric acid ( L -ABA) as a precursor for the anticonvulsant and the antituberculotic is a key intermediate in the chemical and pharmaceutical industries. Recently, leucine dehydrogenase (LeuDH) with NAD + regeneration was developed for L -ABA production on a large scale. Previously, the L -ABA yield was improved by optimizing conversion conditions, including cofactor regeneration and enzyme immobilization but not protein engineering on LeuDH due to lacking an applicable high-throughput screening (HTS) method. Recently, an HTS assay was developed by us, which enables researchers to engineer LeuDH in a relatively short period of time. Herein, a semirational engineering was performed on LeuDH to increase the catalytic efficiency of BcLeuDH. Firstly, the structure of wild-type (WT) BcLeuDH was modeled and seven potentially beneficial positions were selected for mutation. Five beneficial variants were then identified from the seven site-saturation mutagenesis (SSM) libraries by HTS and confirmed by rescreening via amino acid analyzer. The “best” variant M5 (WT + Q358N) showed 44.5-fold higher catalytic efficiency ( k
cat / K
M ) than BcLeuDH WT, which suggested that BcLeuDH M5 is an attractive candidate for L -ABA production on a large scale. Furthermore, the structure-functional relationship was investigated based on the docking and kinetic results.
研究领域:
生物技术
·
生物化工
·
酶学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Ruijin Zeng,Minghao Qiu,Qing Wan,Zhisheng Huang,Xiaolong Liu,Dianping Tang,Dietmar Knopp
Large-scale, rapid, and inexpensive serological diagnoses of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) are of great interest in reducing virus transmission at the population level; however, their development is greatly plagued by the lack of available point-of-care methods, leading to low detection efficiency. Herein, an ultrasensitive smartphone-based electrochemical immunoassay is reported for rapid (less than 5 min), low-cost, easy-to-implement detection of the SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 N protein). Specifically, the electrochemical immunoassay was fabricated on a screen-printed carbon electrode coated with electrodeposited gold nanoparticles, followed by incubation of anti-N antibody (Ab) and bovine serum albumin as the working electrode. Accompanied by the antigen–antibody reaction between the SARS-CoV-2 N protein and the Ab, the electron transfer between the electroactive species [Fe(CN)6]3–/4– and the electrode surface is disturbed, resulting in reduced square-wave voltammetry currents at 0.075 V versus the Ag/AgCl reference electrode. The proposed immunoassay provided a good linear range with SARS-CoV-2 N protein concentrations within the scope of 0.01–1000 ng/mL (R2 = 0.9992) and the limit of detection down to 2.6 pg/mL. Moreover, the detection data are wirelessly transmitted to the interface of the smartphone, and the corresponding SARS-CoV-2 N protein concentration value is calculated and displayed. Therefore, the proposed portable detection mode offers great potential for self-differential diagnosis of residents, which will greatly facilitate the effective control and large-scale screening of virus transmission in resource-limited areas.
研究领域:
医学诊断
·
生物传感器
·
病毒学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Yaohua Yang,Kai Yan,Jingdong Zhang
A Ni(OH) 2 /TiO 2 photoanode possessing high photoelectrocatalytic activity toward glucose oxidation driven by visible light was fabricated. The results indicated that deposition of Ni(OH) 2 on TiO 2 film electrode could remarkably promote the catalytic oxidation of glucose under visible light illumination. By applying a low voltage, the Ni(OH) 2 /TiO 2 electrode showed a linear photoelectrochemical response to glucose in the concentration range from 0.5 μM to 20 μM. Furthermore, the high photoelectrocatalytic activity of Ni(OH) 2 /TiO 2 electrode toward glucose oxidation was utilized to construct a glucose-air photofuel cell, which generated electrical signal responding to glucose concentration from 5 μM to 100 μM, without using external electrical power supply. Thus, dual non-enzymatic glucose sensors based on photocatalytic oxidation of glucose on the Ni(OH) 2 /TiO 2 electrode under visible light illumination were demonstrated.
研究领域:
生物医学工程
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Wei Chen,Jiayue Dong,Sihan Zhou,Chunyong Zhang,Degang Fu
1-naphthol (1-NAP) and 2-naphthol (2-NAP) are both hazardous pollutants which pose great threat to our living environment. In the current work, a comparative investigation on the electrochemical mineralization of 1-NAP and 2-NAP using boron-doped diamond (BDD) anode was presented. Experiments were carried out using synthetic 1-NAP or 2-NAP solutions containing Na 2 SO 4 as supporting electrolyte . A two-level factorial design methodology was adopted to optimize the mineralization process, six mathematical models were thus constructed and used to explore the structure-property relation involved. The results obtained suggested that 1-NAP was easier and faster to break down than 2-NPA under the same oxidation conditions, which were in line with the outcome obtained from cyclic voltammetry , HPLC and TOC measurements. To understand the destruction mechanism, the electron structures of 1-NAP and 2-NAP were further examined by the quantum chemistry calculations with density functional theory. It was also found that the results from frontier orbital analysis could well explain the differing mineralization performances recorded in BDD anode cell. Finally, with the aid of LC/MS analysis, the oxidation pathways of 1-NAP or 2-NAP on BDD anodes were proposed.
研究领域:
环境科学
·
电化学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2019
·Vol. 103
·期 2
·1046-1056
·
IF 3.094
Xiaohui Feng,Le Wang,Ran Wei,Ziyan Wang
Non-contact temperature sensors based on the fluorescence intensity ratio (FIR) have been widely investigated owing to their high sensitivity and reliable real-time monitoring. Herein, the SiO 2 -coated LiY(MoO 4 ) 2 @SiO 2 :Er 3+ ,Yb 3+ phosphor was investigated as an optical thermometry material, which was synthesized using the conventional solid state reaction and coated by a facile wet chemical route. The effect of surface modification on FIR was systematically characterized by structural analyses and spectral measurements and the temperature-dependent up-conversion FIR was investigated from 303 to 603 K under a 980 nm laser excitation. The results showed that the FIR value was thermally stable and the SiO 2 coating led to a higher FIR sensitivity as well as a higher saturation threshold. This work would pave a way to design interesting optical thermometry materials in up-conversion phosphors with better properties.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
DALTON TRANSACTIONS
·
2019
·Vol. 49
·期 12
·3743-3749
·
IF 4.052
Ruling Tang,Chunli Hu,Jianghe Feng,Zhi Fang,Yan Chen,Fang Kong,Jianggao Mao
Two new inorganic metal borate derivatives, Sr5TeO2(BO3)4 and NaSr5(BO3)(SiO4)2, have been obtained through a high temperature solid reaction method. Sr5TeO2(BO3)4 crystallizes in a tetragonal space group of P4/mnc featuring a three-dimensional (3D) structure composed of a porous 3D strontium framework stabilized by the unique zero-dimensional (0D) [TeO2(BO3)4]10− anions. NaSr5(BO3)(SiO4)2 crystallizes in a monoclinic space group of P21/c with a 3D structure consisting of a splendid strontium 3D framework strengthened by isolated BO3 and SiO4 groups. The sodium cations located in the empty space of the structure. Thermal stability analyses, optical property measurements and theoretical studies of the compounds were performed. Refractive index calculations revealed that the birefringence of Sr5TeO2(BO3)4 and NaSr5(BO3)(SiO4)2 is 0.048 and 0.029, respectively, at 1064 nm.
研究领域:
材料科学
·
无机化学
✓ 已验证
DOI
高影响因子
Chem Catalysis
·
2022
·
IF 0
Yu Li,Peiyuan Yao,Shiqing Zhang,Jinhui Feng,Hao Su,Xiangtao Liu,Xiang Sheng,Qiaqing Wu,Dunming Zhu,Yanhe Ma
Summary While formaldehyde is an emerging C1 resource for the synthesis of high value-added chemicals due to its high and versatile reactivity, selective activation and controllable transformation of formaldehyde still remains a great challenge. In particular, coupling of formaldehyde with small aldehydes such as 3-hydroxypropanal offers excellent opportunity for constructing valuable molecules but has not been realized. A benzaldehyde lyase from Herbiconiux sp. SALV-R1 was engineered through directed evolution to achieve the coupling of 3-hydroxypropanal and formaldehyde. A carbonyl reductase and a transaminase were identified to catalyze reduction or transamination of 1,4-dihydroxybutan-2-one, a highly functionalized small ketone. The newly attained enzymatic hydroxymethylation and asymmetric reduction or transamination were then combined with the chemocatalytic hydration of acrolein in a one-pot process to synthesize optically pure 1,2,4-butanetriol and 2-aminobutane-1,4-diol from acrolein and formaldehyde. These results exemplify a new way for sustainable synthesis of high value-added chemicals from C1 molecules and simple starting materials.
研究领域:
化学工程
·
生物化学
·
生物技术
·
有机化学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）