SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Kai Zhang,Meina Yang,Helin Sun,Xueyin Wang,Hao Li,Jinxiang Han,Zhongwen Zhang
Using Peptide nucleic acid (PNA), this work provides a unique approach for controlling CRISPR/Cas13a activity by RNA Inhibitory PNA (RIP). This method reduces the action of CRISPR/Cas13a by inhibiting the binding of crRNA to CRISPR/Cas13a, which was subsequently utilized to detect metalloproteinase-2 (MMP-2) activity. We carefully constructed a PNA sequence that includes a peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) that is particularly cleaved by MMP-2 in the middle. When MMP-2 is not present in the system, the PNA/crRNA complex reduces crRNA binding to CRISPR/Cas13a, hence inhibiting CRISPR/Cas13a cleavage activity. When MMP-2 is present in the system, it cleaves the peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) selectively and so prevents PNA from binding to crRNA, enabling crRNA to reach the "free" form. Then, in the presence of an RNA activator, crRNA may bind to CRISPR/Cas13a and stimulate its activity. CRISPR/Cas13a triggered in this manner may precisely cleave the DNA sequence containing modified -UU- bases on the electrode surface . Thus, the ferrocene (Fc) is changed away from the electrode surface at one end of the DNA , enabling for the recovery of the Electrochemiluminescence (ECL) signal from the two-dimensional material transformed on the electrode surface. As a result, MMP-2 activity was detected utilizing this technique of inhibiting crRNA activity with a detection limit of 15.56 fM. Our suggested method of inhibiting CRISPR/Cas13a activity via PNA has a broad variety of potential therapeutic applications in gene editing and the development of CRISPR/Cas13a-based biosensors.
研究领域:
分子生物学
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基因编辑
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生物传感技术
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高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
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2023
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IF 2.4
Ping Zhao,Qingle Zeng
We have developed a diversity-oriented, substrate-controlled, chemoselective synthetic method for a variety of structurally diverse 1-sulfonylquinazoline-2,4(1H,3H)-diones and 2-sulfonamidobenzonitriles. This substrate-controlled synthesis is accomplished through the condensation reaction of 2-(sulfonamido)benzamides with or without an N-methyl group on the amides. The reactions are fulfilled within half an hour at room temperature with solid triphosgene as a convenient condensing agent and pyridine as an acid acceptor. This protocol features easily available starting materials, mild reaction conditions, easy experimental operation, and moderate to excellent yields of products.
研究领域:
有机化学
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药物化学
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DOI
高影响因子
PHYTOCHEMISTRY
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2024
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IF 3.2
Hao-Dong Yang,Yuan-Gui Yang,Zhi-Shu Tang,Kang Ma,Hong-Bo Xu
Acanthopanacis cortex (the dried root bark of Acanthopanax gracilistylus W. W. Smith) has been used for the treatment of rheumatic diseases in China for over 2000 years. Four previously undescribed lignans ( 1 – 4 ) and 12 known lignans ( 5 – 16 ) were isolated from Acanthopanacis cortex. In this study, the inhibitory activities of compounds 1 – 16 against neutrophil elastase (NE), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) are reported. The results show that compounds 1 – 16 exhibit weak inhibitory activities against NE and COX-1. However, compounds 2 , 6 – 8 and 13 – 16 demonstrate better COX-2 inhibitory effects with IC 50 values from 0.75 to 8.17 μ Μ. These findings provide useful information for the search for natural selective COX-2 inhibitors.
研究领域:
药理学
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中药学
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天然产物化学
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2022
·Vol. 32
·期 48
·2209743
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IF 19.924
Yi-Xuan Li,Jing Li,Dunru Zhu,Ju-Zheng Wang,Guo-Fang Shu,Junji Li,Sheng-Li Zhang,Xue-Ji Zhang,Serge Cosnier,Hai-Bo Zeng,Dan Shan
High electrocatalytic activity with tunable luminescence is crucial for the development of electrochemiluminescence (ECL) luminophores. In this study, a porphyrin-based heterobimetallic 2D metal organic framework (MOF), [(ZnTCPP)Co 2 (MeIm)] (1), is successfully self-assembled from the zinc(II) tetrakis(4-carboxyphenyl)porphine (ZnTCPP) linker and cobalt(II) ions in the presence of 2-methylimidazole (MeIm) by a facile one-pot reaction in methanol at room temperature. On the basis of the experimental results and the theoretical calculations, the MOF 1 contains paddle–wheel [Co 2 (-CO 2 ) 4 ] secondary building units (SBUs) axially coordinated by a MeIm ligand, which is very beneficial to the electron transfer between the Co(II) ions and oxygen. Combining the photosensitizers ZnTCPP and the electroactive [Co 2 (-CO 2 ) 4 ] SBUs, the 2D MOF 1 possesses an excellent ECL performance, and can be used as a novel ECL probe for rapid nonamplified detection of the RdRp gene of SARS-CoV-2 with an extremely low limit of detection (≈30 aM).
研究领域:
材料科学
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分析化学
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病毒学
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PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
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2025
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IF 17.7
Pengkun Zhang,Qinhan Wu,Haoyu Wang,Dong-Hau Kuo,Yujie Lai,Dongfang Lu,Jiqing Li,Jinguo Lin,Zhanhui Yuan,Xiaoyun Chen
Herein, we established a Zn 3 (OH) 2 (V 2 O 7 )(H 2 O) 2 /V-Zn(O,S) Z-scheme heterojunction (labeled ZnVO/V-Zn(O,S) with a heterovalent V 4+ /V 5+ states and oxygen vacancies in both phases via a one-step in-situ hydrolysis method. The NaBH 4 regulated the ZnVO/V-Zn(O,S)-3 with rich Vo and suitable n (V 4+ )/ n (V 5+ ) ratio achieved an excellent photocatalytic nitrogen fixation activity of 301.7 μmol/(g·h) and apparent quantum efficiency of 1.148% at 420 nm without any sacrificial agent, which is 11 times than that of V-Zn(O,S). The Vo acts as the active site to trap and activate N 2 molecules and to trap and activate H 2 O to produce the H for N 2 molecules photocatalytic reduction. The rich Vo defects can also reduce the competitive adsorption of H 2 O and N 2 molecules on the surface active site of the catalyst. The heterovalent vanadium states act as the photogenerated electrons, quickly hopping between V 4+ and V 5+ to transfer for the photocatalytic N 2 reduction reaction. Additionally, the Z-scheme heterojunction effectively minimizes photogenerated carrier recombination. These synergistic effects collectively boost the photocatalytic nitrogen fixation activity. This study provides a practical method for designing Z-scheme heterojunctions for efficient photocatalytic N 2 fixation under mild conditions.
研究领域:
材料科学
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催化
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环境科学
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纳米技术
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化学工程
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DOI
高影响因子
Journal of Coatings Technology and Research
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2025
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IF 2.8
Liu Jinkang,Lv Ziqi,Zhao Yunyan,Li Guicun,Zhang Zhonghua,Song Xibin,Waterhouse Geoffrey I. N.
Self-healing anticorrosion coatings are attractive for extending the service-life of metals, especially in marine environments. This work reports the novel self-healing coatings based on (2-mercaptobenzimidazole)-loaded carbon nanocages (MBI@CNCs) embedded in a polymethyl methacrylate (PMMA) matrix. The carbon nanocages (CNCs) as inhibitor carriers provide abundant channels for loading and releasing of 2-mercaptobenzimidazole (MBI). Nanocomposite coatings containing 12 wt.% MBI@CNCs deliver the optimal anticorrosion performance as well as excellent self-healing properties when applied to carbon steels. After scratching the coatings in a corrosive medium, MBI molecules are released from the coating. The corrosion inhibitor then reacts with the metal surface to form a passivation layer by coordination and electrostatic effect. The general strategy combining the one-pot synthesis of CNCs and loading with a corrosion inhibitor is conducive to large-scale production and practical application of self-healing anticorrosion coatings.
研究领域:
材料科学
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腐蚀与防护
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纳米技术
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DOI
高影响因子
Food & Function
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2022
·Vol. 13
·期 19
·9931-9946
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IF 6.317
Xiyi Hu,Zhenguo Liu,Yuntao Lu,Xuepeng Chi,Kai Han,Hongfang Wang,Ying Wang,Lanting Ma,Baohua Xu
10-Hydroxy-2-decenoic acid (10-HDA) is a principal active ingredients of royal jelly. Several recent studies demonstrated that 10-HDA has potential anti-type 2 diabetes mellitus (T2DM) properties. To evaluate the anti-T2DM effect of 10-HDA and explore its underlying molecular mechanisms, we used high fat diet (HFD) combined with streptozotocin (STZ) injection to establish a diabetes model. Mice were randomly divided into four groups (8 mice per group): control group, 10-HDA group, T2DM group, and T2DM + 10-HDA group. The 10-HDA and T2DM + 10-HDA groups were administered intragastric 10-HDA (100 mg per kg body weight), while the control and T2DM groups were administered a vehicle, daily for 4 weeks. Our analysis indicated that there was no significant difference in body weight between T2DM + 10-HDA and control group mice (P > 0.05). Treatment with 10-HDA reduced fasting blood glucose and increased insulin levels in diabetic mice (P < 0.05), as well as increasing the area of pancreatic islets (P < 0.05), and alleviating vacuolar degeneration in the liver. Further, 10-HDA intervention increased superoxide dismutase, catalase, and glutathione peroxidase activities in diabetic mouse liver, alleviated lipid peroxidation, inhibited liver NF-κB nuclear translocation, decreased IL-6 and TNF-α content, and increased P-PI3K, P-AKT, and P-GSK3β protein levels (all P < 0.05). Fifteen potential biomarkers were screened by analysis of liver metabolomics data, of which hexadecanamide, stearamide, pentadecanoic acid, and fatty acid esters of hydroxy fatty acids (16:0/18:1) were highly abundant. In conclusion, 10-HDA has clear hypoglycemic effects on diabetic mice, through the PI3K/AKT/GSK3β signaling pathway.
研究领域:
药理学
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内分泌学
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代谢学
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分子生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2019
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IF 7.149
Wei Guo,Jian Han,Shengmin Wu,Xiongjie Shi,Qiangwei Wang,Bingsheng Zhou
Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (TBPH) is a ubiquitous environmental contaminant, but its toxicity is not fully understood. Accordingly, we investigated the effects of TBPH and its metabolite, mono-(2-ethyhexyl)tetrabromophthalate (TBMEHP), on lipid metabolism using a zebrafish model. The molecular docking study revealed that TBPH and TBMEHP bind to zebrafish peroxisome proliferator-activated receptor γ (PPARγ), with binding energies similar to rosiglitazone, a PPARγ agonist. Zebrafish embryos 0.75 hpf were exposed to TBPH (0.2–2000 nM) or TBMEHP (0.2–2000 nM) until 72 hpf, and their effects on PPARγ-mediated lipid metabolism were evaluated. Significant regional DNA demethylation of the PPARγ promoter was observed in the larvae at 72 hpf. Demethylation of the PPARγ promoter accompanied by upregulation of tet1 and tet2 transcription caused upregulation of PPARγ transcription and certain downstream genes involved in lipid lipolysis, transport, and metabolism. The triglyceride and total cholesterol concentrations in the larvae were significantly reduced following exposure to TBPH or TBMEHP. Furthermore, significant increases in the whole ATP content and locomotor activity in the 120 hpf larvae were observed. The overall results suggest that both TBPH and TBMEHP affect methylation of the PPARγ promoter, subsequently influencing larvae lipid metabolism via the PPARγ signaling pathway and disrupting energy homeostasis.
研究领域:
环境毒理学
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分子生物学
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发育生物学
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表观遗传学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2019
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IF 3.845
Lin Xiao-mian,Luo Wu,Wang Heng,Li Rong-zhen,Huang Yi-shan,Chen Lian-kuai,Wu Xiao-ping
The prostaglandin-endoperoxide synthase-2 (PTGS2) plays essential roles in diverse pathological process. Although recent studies implied that PTGS2 was closely related with chemoresistance, the precise roles and the underlying mechanisms of PTGS2 in the developing process of chemoresistance in non-small cell lung cancer (NSCLC) remained elusive. In the present study, we revealed a novel molecular mechanism of PTGS2 implicated in the chemoresistance of NSCLC and proposed a model for the positive feedback regulation of PTGS2 in the process of developing resistance phenotype in NSCLC cells. Our results demonstrated that cisplatin induced PTGS2 expression through the ROS-ERK1/2-NF-κB signaling axis. The prostaglandin E2 (PGE2) derived from PTGS2 catalyzation further strengthened PTGS2 expression via the PGE2-EPs-ERK1/2 positive feedback loop, which induced multidrug resistance of NSCLC cells through up-regulation of BCL2 expression and the subsequent attenuation of cell apoptosis. Consistently, high levels of both PTGS2 and BCL2 were closely associated with poor survival in NSCLC patients. Inhibition of PTGS2 significantly reversed the chemoresistance in the resistant NSCLC in vitro and in vivo. Our results suggested that PTGS2 might be employed as an adjunctive therapeutic target for improving the response to the therapeutic agents in a subset of resistant NSCLC.
研究领域:
肿瘤学
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分子生物学
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药理学
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PubMed
高影响因子
NEUROREPORT
·
2025
·Vol. 36
·期 16
·988
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IF 1.7
Zhong Zhihong,Xu Canxin,Chen Xiao,Lin Dong,Bian Liuguan
Plain Language Summary Background Intracerebral hemorrhage (ICH) induces secondary brain injury, driven in part by oxidative stress caused by hemin, a toxic hemoglobin breakdown product. Cortical astrocytes, critical for maintaining redox homeostasis, are vulnerable to hemin-induced oxidative damage, exacerbating neuronal injury. Induced pluripotent stem cell–derived neural progenitor cells (iPSC-NPCs) have shown therapeutic potential in brain injury models by promoting neural repair, but their ability to protect astrocytes against hemin toxicity remains unexplored. We hypothesized that iPSC-NPCs coculture mitigates hemin-induced oxidative stress in astrocytes by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) antioxidant pathway. Materials and methods Astrocytes were exposed to hemin with or without iPSC-NPC coculture. We assessed cell viability, reactive oxygen species (ROS) accumulation, apoptosis, and the role of the Nrf2/HO-1 pathway using small-interfering RNA. Results iPSC-NPC coculture significantly reduced hemin-induced oxidative damage by promoting Nrf2 nuclear translocation and upregulating HO-1, thereby decreasing ROS and apoptosis. Silencing Nrf2 abolished these protective effects. Conclusion Our findings demonstrate that iPSC-NPCs protect astrocytes from hemin toxicity via the Nrf2/HO-1 pathway, suggesting a novel therapeutic strategy for ICH-induced oxidative injury. Plain Language Summary Intracerebral hemorrhage (ICH) can cause brain damage due to oxidative stress from hemin, a toxic breakdown product of hemoglobin. This study explored whether induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs) can protect astrocytes, which are crucial for brain health, from hemin-induced damage. Researchers found that when astrocytes were cocultured with iPSC-NPCs, oxidative stress and cell death were reduced. This protective effect was linked to the activation of the Nrf2/HO-1 antioxidant pathway. Silencing Nrf2 negated the benefits, highlighting the pathway's importance. These findings suggest iPSC-NPCs could be a promising treatment for oxidative injury in ICH. Text is machine generated and may contain inaccuracies. FAQ
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Hao-Min Song,Li-Xia Zhao,Shuai-Qi Zhang,Tong Ye,Ying Fu,Fei Ye
Cinnamic acid, isolated from cinnamon bark, is a natural product with excellent bioactivity, and it effectively binds with cyclohexanedione to form novel 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors. According to the active sub-structure combination principle, a series of novel 3-hydroxy-2-cinnamoyl-2-en-1-one derivatives were designed and synthesized. The title compounds were characterized by infrared, 1H NMR, 13C NMR, and HRMS. The in vitro inhibitory activity of AtHPPD verified that compound II-13 showed the most activity with a half-maximal inhibitory concentration (IC50) value of 0.180 μM, which was superior to that of mesotrione (0.206 μM) in vitro. The preliminary herbicidal activity tests demonstrated that some compounds had good herbicidal activity especially compound II-13 at a concentration of 150 g ai/ha. The binding mode of AtHPPD through molecular docking indicated that two oxygens of compounds II-13 formed bidentate interactions with metal ions, and the benzene ring formed π-π accumulation effects with Phe-381 and Phe-424. The results of molecular dynamics simulations showed that compound II-13 exhibited a more stable binding ability with AtHPPD than mesotrione. This study provided insights into the development of natural and efficient herbicides in the future.
研究领域:
化学
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药理学
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农业科学
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分子生物学
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植物科学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2024
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IF 1.6
Dapeng Sun,Chenglong Ye,Zhuo Chen
Matrix metalloproteinase-2 (MMP-2) is a key biomarker involved in various pathological processes, so its sensitive and selective detection is of great clinical significance. A fluorescent sensor for the detection of MMP-2 was successfully designed based on the inner filter effect (IFE) between carbon dots (CDs) and gold nanoparticles (AuNPs). In the presence of MMP-2, peptide chains are hydrolyzed, releasing short peptides that contain thiol groups and positive charges, thereby inducing the aggregation of AuNPs. This aggregation disrupts the IFE between AuNPs and CDs, leading to a significant enhancement of the fluorescence. By monitoring the change in fluorescence intensity of CDs, the concentration of MMP-2 was accurately determined. The sensor exhibited a linear response across the concentration range from 1.0 to 150 ng/mL, with a detection limit as low as 0.23 ng/mL, which is lower than those of previously reported methods. Furthermore, the sensor demonstrated minimal interference from coexisting biochemical substances for MMP-2 in human serum. Notably, the sensor was successfully applied to determine MMP-2 in human serum, showing satisfactory recovery rates ranging from 98.8 to 1106.0%. In conclusion, this fluorescent sensor offers high selectivity and sensitivity for MMP-2 detection, making it a promising tool for clinical diagnosis of related diseases.
研究领域:
生物医学
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纳米技术
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临床诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）