POSTHARVEST BIOLOGY AND TECHNOLOGY
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2026
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IF 7.3
Yue Shi,Shiwei Shi,Yangmei Liu,Yonghua Zhang,Xiumei Chen,Nengguo Tao,Qiuli OuYang
ACL is identified as the target of trans-2-enals in suppressing G. citri-aurantii . • Trans-2-enals bind ACL with high affinity by Lys76 and Arg84. • ACL is a target for trans-2-enals (C₅-C₁₀), enabling broad antifungal strategies. Postharvest sour rot caused by Geotrichum citri-aurantii severely compromises citrus quality and marketability. This study clarified the antifungal mechanism of trans-2-enals (C 5 –C 10 ) against G. citri-aurantii , using trans-2-hexenal as a model. Physiological and transcriptome analysis of G. citri-aurantii treated with trans-2-hexenal revealed mitochondrial dysfunction and pinpointed ATP-citrate lyase (ACL) as a key inhibited target within the tricarboxylic acid (TCA) cycle. Enzymatic assays and RT-qPCR confirmed ACL inhibition, leading to citric acid accumulation and depletion of downstream intermediates. Density-functional analysis identified the conjugated enal moiety as the essential pharmacophore for trans-2-enals. Crucially, molecular docking localized a high-affinity binding cavity on ACL, where trans-2-enals form critical hydrogen bonds with residues Lys76 and Arg84. All tested trans-2-enals were confirmed to suppress ACL activity and expression, thereby altering TCA cycle intermediates. Collectively, ACL is the primary target of trans-2-enals, providing a foundation for developing next-generation antifungal agents against G. citri-aurantii . Download: Download high-res image (192KB) Download: Download full-size image Created with BioGDP.com ( Jiang et al., 2025 ).
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高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 1
·419-426
·
IF 3.719
He Yunfei,Liao Lifu,Xu Canhui,Wu Rurong,Li Shijun,Yang Yanyan
We describe a resonance light scattering (RLS) method for the direct detection of adenosine triphosphate (ATP) in aqueous solution without the need for separation. The binuclear uranyl complex bis-uranyl-bis-sulfosalophen (BUBSS) was synthesized by the coordination reaction of two uranyl ions with the ditopic tetradentate ligand bis-sulfosalophen. In parallel, gold nanoparticles were modified with anti-ATP aptamer (Apt-AuNPs) to serve as RLS probes. The detection scheme is based on the specific reaction of a BUBSS molecule with two ATP molecules to form a linear supramolecule. This, in turn, binds two Apt-AuNPs molecules, and this is accompanied by the aggregation of the Apt-AuNPs and results in a strong RLS signal. A RLS method was developed on this basis that has a linear response to ATP in the 5 to 500 nM concentration range, with a 4.5 nM detection limit under optimal conditions. The method has been successfully applied to the detection of ATP in spiked real samples and gave recoveries between 96.3 and 102.5 %.
We describe a resonance light scattering (RLS) method for the direct detection of adenosine triphosphate (ATP) in aqueous. Gold nanoparticles were modified with anti-ATP aptamer (Apt-AuNPs) to serve as RLS probes. The detection scheme is based on the specific reaction of binuclear uranyl complex molecule with two ATP molecules to form a linear supramolecule.
研究领域:
分析化学
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生物化学
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纳米技术
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
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IF 4.4
Dechan Lu,Rongyuan Cai,Yuqin Liao,Ruiyun You,Yudong Lu
Abnormal uric acid (UA) content in body fluids can fully reflect the status of metabolism and immunity in the body. We have developed a simple, efficient and label-free surface enhanced Raman scattering (SERS) method for UA detection. Briefly, p-aminothiophenol (p-ATP) was used as the internal standard molecule and linking molecule to prepare a glass/p-ATP/Ag NPs SERS substrate. The Raman characteristic peak of p-ATP at 1076 cm −1 can be used as an internal standard molecule to correct the signal fluctuation of UA detection. The results show that the SERS method owns a linear response with a ranging from 5 × 10 -6 to 10 -3 M of UA characteristic peak of both 693 cm −1 and 493 cm −1 with a determination coefficient (R 2 ) of 0.9878 and 0.9649, respectively. Additionally, the SERS sensor has been further used for the analysis of UA in sweat and good recoveries were obtained for the sensing of sweat. We believe that the developed SERS substrate has potential for applications in healthcare monitoring.
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PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Liu Jianming,Zhang Han,Xu Yingying,Meng Hao,Zeng An-Ping
The use of gaseous and air-captured CO 2 for technical biosynthesis is highly desired, but elusive so far due to several obstacles including high energy (ATP, NADPH) demand, low thermodynamic driving force and limited biosynthesis rate. Here, we present an ATP and NAD(P)H-free chemoenzymatic system for amino acid and pyruvate biosynthesis by coupling methanol with CO 2 . It relies on a re-engineered glycine cleavage system with the NAD(P)H-dependent L protein replaced by biocompatible chemical reduction of protein H with dithiothreitol. The latter provides a higher thermodynamic driving force, determines the reaction direction, and avoids protein polymerization of the rate-limiting enzyme carboxylase. Engineering of H protein to effectively release the lipoamide arm from a protected state further enhanced the system performance, achieving the synthesis of glycine, serine and pyruvate at g/L level from methanol and air-captured CO 2 . This work opens up the door for biosynthesis of amino acids and derived products from air.
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高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Tianyang Liu,Yuying Zhang,Taoli Huhe,Chao Yao,Xiazhang Li
The degradation of large flow volatile organic compounds (VOCs) at low temperature remains a significant challenge. Photothermal catalytic technology, which integrates the advantages of photocatalysis and thermal catalysis, has the potential to address this challenge. In this work, using attapulgite (ATP) as the substrate, MnCo 2 O 4.5 /ATP composites with various spinel loading amount were prepared via a sol-gel approach. Results demonstrated that the 20 % MnCo 2 O 4.5 /ATP catalyst presented the best catalytic performance at a space velocity of 10,000 mL·g −1 h −1 (500 ppm toluene) under light illumination. The toluene conversion rate and mineralization rate reached 99 % and 81 %, respectively. The surface temperature of MnCo 2 O 4.5 /ATP composite rapidly increased under Xenon lamp irradiation, reaching as high as 301.6 °C. In-situ DRIFTS and DFT calculations were performed to explore the photothermal catalytic reaction mechanism. The effective MnCo 2 O 4.5 /ATP interface facilitated the mobility of lattice oxygen and strengthened the activation of oxygen. The ATP surface with its high surface area and rich hydroxyl groups promoted the adsorption of toluene. The oxygen vacancies enriched on the surface of MnCo 2 O 4.5 intensified the adsorption and activation of oxygen, facilitating the transformation of toluene at the MnCo 2 O 4.5 /ATP interface. Current work offers a new strategy for the efficient catalytic elimination of VOCs by taking advantage of solar energy and minerals.
研究领域:
环境科学
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催化材料
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化学工程
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高影响因子
STRUCTURE
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2025
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IF 4.3
Shu-Cheng Fang,Liang Wang,Meng-Ting Cheng,Da Xu,Zhi-Peng Chen,Jie Wang,Wenli Liao,Yanyan Li,Cong-Zhao Zhou,Wen-Tao Hou,Yuxing Chen
The human ATP-binding cassette (ABC) transporter ABCA7 participates in the lipidation of apolipoprotein ApoE, a commonly recognized risk factor for Alzheimer’s disease (AD). How ABCA7 is involved in the molecular pathogenesis of AD remains poorly understood. Using cryoelectron microscopy (cryo-EM), we determined ABCA7 structures in the apo and substrate-bound forms, respectively. Combined with activity assays, we assigned the residues that specifically bind two molecules of phosphatidylserine (PS) that are arranged in a “tail-to-tail” manner. Pull-down assays confirmed that ApoE directly interacts with ABCA7; and moreover, both ATPase and lipid transport activities of ABCA7 were significantly enhanced in the presence of ApoE. We also measured the activities of a familial AD variant and a protective clinically reported variant in the ABCA7 gene. Our findings not only give structural insights into ABCA7-mediated PS translocation, but we also provide first biochemical evidence for its link to AD by forwarding lipids to ApoE.
研究领域:
结构生物学
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神经科学
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分子生物学
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PubMed
高影响因子
PLoS One
·
2017
·Vol. 12
·期 11
·e0186184
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IF 2.806
Tingyu Yu,Jiao Lin,Jin Zhao,Wei Huang,Lingwen Zeng,Zhiyuan Fang,Ning Xu
The analysis of intracellular ATP can reveal the response of cells to different treatments and is important for individualized medicine. In the present study, we developed a cell penetrating peptides (CPPs) tagged luciferase (TAT-LUC) for tumor chemosensitivity assay. The activity of recombinant TAT-LUC was evaluated using ATP standard solution and tumor cells. This recombinant TAT-LUC was then used for the analysis of sensitivity index (SI) of four strains of tumor cells. The results showed that TAT-LUC could detect less than 10 nM extracellular ATP with a strong correlation between the luminescence intensity and the ATP content (R 2 = 0.994). Without cell lysis, the detection limit for intracellular ATP analysis was 40 tumor cells. Furthermore, chemosensitivity of four strains of tumor cells (Skov-3/DDP, A549/DDP, MDA-MB-231, Huh-7) was determined by this assay successfully. The cell penetration ability of TAT-LUC enables the assay not only to reflect drug resistance of tumor cells real-timely but also to minimize the test time, which can be a valuable aid for personalized cancer chemotherapy.
研究领域:
肿瘤学
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分子生物学
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药理学
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PubMed
高影响因子
ACS Nano
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2024
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IF 15.8
Yufei Zhang,Ze-Tao Jiang,Yuxia Wang,Huan-Yu Wang,Shihao Hong,WenBo Li,Dong-Sheng Guo,Xinge Zhang
In the context of an increasingly escalating antibiotics crisis, phototherapy has emerged as a promising therapeutic approach due to its inherent advantages, including high selectivity, noninvasiveness, and low drug resistance. Photothermal therapy (PTT) and photodynamic therapy (PDT) are two complementary and promising phototherapies albeit with inherent limitations, noted as the challenges in achieving precise heat confinement and the associated risk of off-target damage for PTT, while the constraints due to the hypoxic microenvironment are prevalent in biofilms faced by PDT. Herein, we have designed a supramolecular nanoformulation that leverages the complexation-induced quenching of guanidinium-modified calix[5]arene grafted with fluorocarbon chains (GC5AF5), the efficient recognition of adenosine triphosphate (ATP), and the oxygen-carrying capacity of the fluorocarbon chain. This intelligent nanoformulation enables the adaptive enhancement of both photothermal therapy (PTT) and photodynamic therapy (PDT), allowing for on-demand switching between the two modalities. Our nanoformulation utilizes ATP released by dead bacteria to accelerate the elimination of biofilms, rendering bacteria unable to resist while minimizing harm to healthy tissues. This research highlights the particular recognition and assembly capabilities of macrocycles, offering a promising strategy for creating potent, combined antibiofilm therapies.
研究领域:
医学
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药学
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材料科学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Tingsong Ye,Rongwei Cui,Xin Zhou,Yingying Huang,Xin Li,Sami Ramadan,Bing Li,Lizhou Xu,Danyang Li
The existing aptamer-regulated nanozymes for colorimetric detection primarily rely on unidirectional activity modulation. The role of salt ions in regulating nanozyme activity and the underlying mechanisms remain poorly understood, limiting their broader application. Herein, we report a novel bidirectional regulation strategy using a hemin-functionalized oxidized carbon nanotubes (Hemin/oCNT) nanozyme. Contrary to convertional approaches that seek to mitigate salt interference, we here leverage the salt-induced charge screening effect that high salt suppresses the peroxidase-like activity of Hemin/oCNT, yet paradoxically promotes aptamer binding to Hemin/oCNT. The formed aptamer/Hemin/oCNT complex exhibits restored and enhanced activity in high salt, attributed to improved dispersion, facilitated substrate (3,3′,5,5′-tetramethylbenzidine, TMB) adsorption and accelerated electron transfer. This bidirectional mechanism was harnessed to develop a one-step ‘mix-and-detect’ aptasensor for adenosine triphosphate (ATP). The assay achieves a wide linear range of 0.625–50 μM with a low detection limit of 300 nM (UV–vis). Furthermore, a smartphone-assisted dual mode (RGB/CMY, Red-Green-Blue/Cyan-Magenta-Yellow) platform was established, where the R channel achieved a superior detection limit of 190 nM, and the CMY mode possessed excellent precision analysis (RSD ≤ 2 %) owing to its signal stability. This sensor showed high selectivity for ATP and successful application in spiked diluted serum. This work represents a paradigm for designing high-performance biosensors by exploiting, rather than avoiding salt effects.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Shulan Chen,Jiang Huang,Wenyue Chen,Hongwen Wu,Hongliang Tan
Hybridization gating reduces fluorophore ratio-control constraints. • Post-activation dye recruitment limits background from pre-integrated signal dyes. • Immunomagnetic enrichment provides target selectivity for ATP-based sensing. • Chitosan-modified hydrogel limits acid-induced background in portable readout. Rapid bacterial detection in dairy food safety monitoring requires portable platforms that combine analytical sensitivity with target selectivity. Here, we report a hybridization-gated zeolitic imidazolate framework-8 (ZIF-8) ratiometric nanoprobe, designated RSZ, for portable detection of Staphylococcus aureus ( S. aureus ) through adenosine triphosphate (ATP)-triggered DNA duplex signaling. The RSZ probe adopts a dual-zone architecture with Ru(bpy) 3 2+ and ssDNA1 embedded within the ZIF-8 particle interior and ssDNA2 adsorbed on the exterior surface. This architecture separates the stable reference channel from the formation of the responsive fluorescence channel, thereby reducing dependence on precise dual-fluorophore loading and limiting background associated with a pre-integrated responsive fluorophore. Upon ATP-induced framework disruption, ssDNA1 becomes accessible for hybridization with ssDNA2, and the resulting duplex recruits SYBR Green I from solution to generate the responsive fluorescence channel relative to the stable Ru(bpy) 3 2+ reference. To enable field-deployable detection, the nanoprobe is embedded in a chitosan-modified agarose hydrogel in which chitosan maintains a near-neutral microenvironment and limits acid-induced nonspecific activation. Fe 3 O 4 nanoparticles conjugated with porcine immunoglobulin G (Fe 3 O 4 -pIgG) are integrated upstream to selectively capture S. aureus via protein A-pIgG recognition, followed by smartphone red-green-blue analysis without specialized instrumentation. The integrated platform achieves a detection limit of 6 CFU/mL and recoveries of 98.8–101.7% in spiked milk. Therefore, this study demonstrates a hybridization-gated strategy for translating stimulus-induced ZIF-8 disruption into controlled ratiometric signal generation for portable bacterial sensing in food analysis. Download: Download high-res image (106KB) Download: Download full-size image
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高影响因子
PLoS One
·
2019
·Vol. 14
·期 10
·e0223096
·
IF 2.776
Lili Wang,Yunqian Li,Ran Guo,Shanshan Li,Anqi Chang,Zhixiang Zhu,Pengfei Tu
Activated platelets release adenosine trisphosphate (ATP) and bioluminescence analysis of ATP release is usually used to monitor activation of platelets induced by various stimulants. However, bioluminescence analysis of ATP possesses poor linearity, the signal is quickly attenuated, and the accuracy of ATP release from platelets is hard to determine accurately enough to be used in a high throughput screening of platelet inhibitors. The present study was designed to optimize bioluminescence analysis of ATP released by platelets and expand its application in high throughput screening of platelet inhibitors. The results showed that accuracy of ATP analysis was significantly improved by adding coenzyme A (CoA) and signal attenuation of ATP analysis was greatly postponed by adding bovine serum albumin (BSA) both in Hank’s balanced salt solution (HBSS) and Tyrode’s buffer. Furthermore, ATP release of activated platelets and inhibitory effects of Ly294002 and Staurosporine on platelet activation were accurately determined by our optimized bioluminescence analysis of ATP. Thus, we have successfully constructed an optimized bioluminescence analysis of ATP which can be used in high throughput screening of platelet inhibitors.
研究领域:
药理学
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血液学
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分子生物学
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PubMed
高影响因子
Surfaces and Interfaces
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2024
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IF 5.7
Xiaoqian Chen,Xingliang Chen,Binglin Li,Jixian Ma,Binxia Zhao,Nan Shao,Yuqi Wang
A magnetic ternary double Z-scheme ATP-g-C 3 N 4 /SnFe 2 O 4 /Bi 2 WO 6 (AG/SFO/BWO) heterojunction was prepared by a simple and green hydrothermal process, which was successfully used in the dye degradation . AG/7.5 %SFO/BWO catalyst demonstrated outstanding visible-light-induced degradation, achieving an impressive 96.72 % degradation of rhodamine B (RhB) in 2 h. Compared with Bi 2 WO 6 (BWO) and SnFe 2 O 4 /Bi 2 WO 6 (SFO/BWO), the degradation efficacy was increased about 1.65 and 1.16 times, respectively. After being used for five batches, the degradation rate of RhB remained over 90 %. Moreover, AG/7.5 %SFO/BWO showcased remarkable efficacy in degrading methylene blue (MB), Congo red (CR), and tetracycline (TC). Bacterial toxicity test indicated the toxicity of dyes was rapidly decreased in 24 h. Physicochemical properties and morphological characteristics of the prepared photocatalysts were investigated by various methods, including XRD , FTIR, XPS , SEM, TEM , BET, VSM , UV–vis, and EIS . AG/7.5 %SFO/BWO, utilizing a dual-Z scheme pathway, efficiently absorbed broad light, yielding multiple active agents, primarily hole (h + ) and superoxide radical •O 2 − . The double Z-scheme heterojunction structure enhanced the electron-hole separation and ensured a stable photochemical environment. UPLC-HRMS was used to identify intermediates during the RhB degradation. Its degradation pathway and catalytic mechanism were illustrated systematically. The bio-toxicity (acute toxicity, developmental toxicity, and mutagenicity) of the intermediates was also evaluated by T.E.S.T.
研究领域:
环境科学
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材料科学
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高影响因子
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