TALANTA
·
2016
·
IF 4.035
Jie Tang,Peng Wu,Xiandeng Hou,Kailai Xu
A facile yet sensitive and selective method was proposed for Hg 2+ detection based on N-acetyl-L-cysteine(NAC)-induced colorimetric response of AuNPs. The proposed method can be easily performed by introducing the premixing of NAC and Hg 2+ into as-prepared citrate-capped AuNPs solution. A combination of experimental and theoretical studies was applied to illustrate the mechanism of this AuNPs colorimetric system. The strong interaction of NAC and AuNPs through Au–S bond could lead to the aggregation of AuNPs, but the formation of NAC-Hg-NAC complex decreased the affinity between NAC and AuNPs and resulted in an anti-aggregation effect. Therefore, the color of the AuNPs solution would progress from purple to red with the increase of Hg 2+ concentration. The proposed method had a high sensitivity with a limit of detection of 9.9 nM. Coexistent metal ions , including Cd 2+ , Mn 2+ , Al 3+ , Ag + , K + , Mg 2+ , Ca 2+ , Cr 3+ , Cu 2+ , Fe 3+ , Pb 2+ , Ni 2+ and Zn 2+ , did not interfere with the detection of Hg 2+ . This method can be used to monitor Hg 2+ in tap water.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Tianxing Fang,Qingyi Wei,Erwen Wu,Hongbin Pu
Malachite green (MG)-contaminated aquatic products pose a serious threat to animal and human health. Hence, a novel recyclable surface-enhanced Raman scattering (SERS) substrate based on AuNPs/TiO 2 /Ti 3 C 2 heterostructures was developed for the detection and degradation of MG in aquatic products. Specifically, AuNPs/TiO 2 /Ti 3 C 2 heterostructures were synthesized by in situ oxidation and electrostatic adsorption based on Ti 3 C 2 nanosheets. The excellent photocatalytic and SERS performance of the AuNPs/TiO 2 /Ti 3 C 2 was demonstrated by Density functional theory (DFT) calculations and experimental results, which was attributed to the enhancement of charge transfer (CT) after the formation of heterostructures. The results demonstrate that AuNPs/TiO 2 /Ti 3 C 2 is highly sensitive and recyclable. The detection limit of the sensor for MG is 8.91 × 10 −5 mg/L. The sensor can be recycled for five times under the condition of light, and shows satisfactory self-cleaning performance in the food matrix, providing a possible alternative solution for the recyclable detection of MG.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xuanxuan Hao,Zhimin Liu,Yaxin Li,Waner Hou,Tongtong Zheng,Leqian Hu
In this study, a near-infrared (NIR) electrochemiluminescence (ECL) emitter CdIn 2 S 4 was synthesized by a convenient hydrothermal strategy. The ECL properties of the obtained CdIn 2 S 4 were examined in the presence of potassium persulfate coreactant at an excitation potential of − 1.25 V. The low trigger voltage of CdIn 2 S 4 may be ascribed to its lower bandgap. This lower excitation potential could effectively reduce the side-effects induced by high voltages, while maintaining the biological activity of antibodides and antigens. The ECL features of CdIn 2 S 4 were also investigated, demonstrating that CdIn 2 S 4 exhibited excellent NIR characteristics, and surface state transitions led to the ECL emission. Importantly, CdIn 2 S 4 was introduced into ECL and electrochemical impedance spectroscopy (EIS) to establish a dual-mode immunosensing interface. Meanwhile, AuNPs@g-C 3 N 4 with excellent properties were used to promote electron transmission and increase the immobilization amount of zearalenone (ZEA) antibodies in the dual-mode sensing system. The developed immunosensing strategy displayed superior sensing performance in ZEA analysis. The detection limits of ZEA were 30 fg mL −1 (ECL model) and 10 fg mL −1 (EIS model), respectively. The desirable recovery results of ZEA in corn samples prove the application potential of the NIR emitter CdIn 2 S 4 in developing a dual-mode immunosensing system for early real-time use.
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Food Analytical Methods
·
2024
·
IF 2.6
Ma Xiaoyuan,Wei Jinxiang,Jiang Caiyun,Wang Zhouping
Herein, an aptasensor with stable surface-enhanced Raman scattering (SERS) and fluorescence dual-signal output had been developed for the detection of aflatoxin B 1 (AFB 1 ). The quantum dots were encapsulated within silica to ensure the stability of fluorescence signal. The gold nanoparticles (AuNPs) were modified with SERS signal molecule DTNB and the complementary sequence (cDNA) of AFB 1 aptamer and then connected on the surface of silica to obtain the signal probes. In the meantime, Fe 3 O 4 was coated with silica which was modified with aptamer to gain capture probes. The two probes could bind specifically based on the base complementary pairing principle. Then, the mixture was separated and gathered by an external magnet. At this time, the maximum SERS signal could be detected in the precipitate, and there was no fluorescence signal in the supernatant. Upon introduction of AFB 1 into the system, the signal probes would be released, resulting in fluorescence recovery in the supernatant while SERS signal weakened in the precipitate. The developed method showed a linear relationship of AFB 1 for the SERS signal in the range of 10 −4 –10 3 ng/mL, with a detection limit of 0.087 pg/mL. The linear range for the fluorescence signal was from 10 −4 to 10 2 ng/mL, with a detection limit of 0.100 pg/mL. The proposed technique was highly sensitive and accurate, which showed promising application prospects in food safety detection.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiayue Zhou,Dongmei Li,Jie Nan,Nan Zhang,Hongwei Zhao,Huan Xia,Zhuxin Chang,Na Sai
Inspired by the desert beetle, a novel biomimetic chip was developed to detect chloramphenicol (CP). The chip was characterized by a periodic array in which hydrophobic Au nanoparticles (AuNPs) were semi-embedded on hydrophilic polymethyl methacrylate (PMMA) spheres. Among them, the AuNPs exhibited both a localized surface plasmon resonance effect to amplify the reflected signal and a synergistic effect with PMMA spheres to create a significant hydrophilic-hydrophobic interface, which facilitated the enrichment of target CP molecules and improved sensitivity. After optimization, the chip showed direct, ultrasensitive (as low as 0.2 ng/mL), fast (5 min), and selective detection of CP with a wide concentration range extending from 0.2 ng/mL to 1000 ng/mL. During detection, color changes of the chip were observed by naked eyes without any color display equipment. The recovery of CP was between 94.65 % and 108.70 % in chicken and milk samples.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yudie Sun,Kehong Quan,Ruilai Zhang,Aobo Feng,Mingfu Ye,Kui Zhang
Methicillin-resistant Staphylococcus aureus (MRSA) is a ubiquitous foodborne pathogen capable of causing food poisoning and producing enterotoxins in foodstuffs, which ultimately results in various illnesses, such as vomiting, diarrhea, and pneumonia. In this study, we present a MoS 2 NF@AuNPs enhanced electrochemiluminescence (ECL) biosensor for MRSA detection based on a DNA branching tree structure formed via enzyme-free hybridization chain reaction (HCR) amplification. This approach integrates bacterial aptamers with multistage HCR amplification to achieve highly sensitive and specific target recognition. Furthermore, MoS 2 NF@AuNPs act as a synergistic catalyst for Ru(bpy) 3 2+ molecules, which in turn substantially enhances the signal readout of the biosensor. The biosensor exhibits a robust linear correlation for MRSA detection over a concentration range of 10 0 to 10 7 cfu/mL, with a detection limit of 1 cfu/mL. In addition, the biosensor demonstrates excellent selectivity and high sensitivity when applied to real samples such as water, juice, and milk.
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Nanomaterials
·
2024
·Vol. 14
·期 19
·1579
·
IF 4.4
Liying Jiang,Dongyang Li,Mingxing Su,Yirong Qiu,Fenghua Chen,Xiaomei Qin,Lan Wang,Yanghai Gui,Jianbo Zhao,Huishi Guo,Xiaoyun Qin,Zhen Zhang
Cardiac troponin I (cTnI) monitoring is of great value in the clinical diagnosis of acute myocardial infarction (AMI). In this paper, a highly sensitive electrochemical aptamer sensor using polystyrene (PS) microspheres as the electrode substrate material in combination with Prussian blue (PB) and gold nanoparticles (AuNPs) was demonstrated for the sensitive and label-free determination of cTnI. PS microspheres were synthesized by emulsion polymerization and then dropped onto the glassy carbon electrode (GCE); PB and AuNPs were electrodeposited on the electrode in corresponding electrolyte solutions step by step. The PS microsphere substrate provided a large surface area for the loading mass of the biological affinity aptamers, while the PB layer improved the electrical conductivity of the modified electrode, and the electroactive AuNPs exhibited excellent catalytic performance for the subsequent electrochemical measurements. In view of the above mentioned AuNPs/PB/PS/GCE sensing platform, the fabricated label-free electrochemical aptamer sensor exhibited a wide detection range of 10 fg/mL~1.0 μg/mL and a low detection limit of 2.03 fg/mL under the optimal conditions. Furthermore, this biosensor provided an effective detection platform for the analysis of cTnI in serum samples. The introduction of this sensitive electrochemical aptamer sensor provides a reference for clinically sensitive detection of cTnI.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xin Xie,Peng Chen,Jingxi Wang,Ding Wang,Daxiu Li,Beibei Kou,Wenjiao Zhou
Herein, a novel electrochemical biosensor on the basis of MB/AuNPs@Zr-MOF signal probe and enzyme-free cascade signal amplification was fabricated for the ultrasensitive detection of colorectal cancer (CRC)-associated miR-92a-3p. Impressively, compared with the traditional electrochemical probes, MB/AuNPs@Zr-MOF not only exhibits high loading for electroactive substances, but also shows enhanced electronic conductivity, thereby generating stable and high electrochemical signal. Meanwhile, under the assistance of the target-induced enzyme-free cascade signal amplification including multicomponent nuclease (MNAzyme) recycle catalytic and catalytic hairpin assembly (CHA), the sensitivity of proposed biosensor was further improved. Additionally, the DNA tetrahedral scaffold (TDNs) was served as an electrode support interface, which could greatly reduce the non-specific adsorption and improve the analytical performance. As a result, the developed biosensor enabled the miR-92a-3p with a detection limit as low as 0.36 fM. This work provided an efficient method to increase the sensitivity and the stability of electrochemical sensing platforms, showing great potential for the detection of biomarker and disease diagnosis.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Longbin Que,Hehua Zhang,Xue Qi,Xin Gu,Hongmin Gao,Yang Zhou,Dong Chang,Hongzhi Pan
Mucin 1 (MUC1), a pivotal tumor biomarker overexpressed in breast cancer, is conventionally detected using laborious, costly, and technically demanding methods. To overcome these limitations, we present a novel label-free electrochemical aptasensor for rapid, sensitive, and cost-efficient MUC1 quantification. The sensor employs a nanohybrid platform composed of gold nanoparticle-decorated three-dimensional cadmium-cobalt oxide nanostructures (3D-CdCo-ONSs@AuNPs), which synergistically amplify the differential pulse voltammetry (DPV) signal. The 3D-CdCo-ONSs provide a high-surface-area scaffold for dense AuNP deposition, enabling efficient immobilization of thiolated aptamers via Au–S bonds. Comprehensive material characterization via scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) confirmed the structural and compositional integrity of the nanocomposite. Under optimized conditions, the sensor exhibited a broad linear detection range from 10 pg/mL to 100 μg/mL (R 2 = 0.998) with an ultralow limit of detection (LOD) of 0.96 pg/mL. The assay demonstrated excellent reproducibility, stability, and selectivity in complex matrices, facilitating precise MUC1 quantification in clinical samples. This work advances the development of portable, high-performance biosensors for early cancer diagnosis and monitoring.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2024
·Vol. 26
·期 5
·1-14
·
IF 2.1
Liu Zhe-Nan,Liu Lv-Cheng,Xue Jin-Peng,Li Shi-Gang,Li Xing
In this work, we employed L-methionine (L-M) as the functionalized reagent of AuNPs for the colorimetric and UV–vis dual-mode detection of As 3+ , Cd 2+ , and Hg 2+ ions with excellent selectivity and anti-interference. After adding the target ions, the functionalized AuNPs can be cross-linked together due to the As 3+ /Cd 2+ /Hg 2+ ions reacting with amino N, carbonyl or hydroxyl O of L-methionine to form the coordination bonds, resulting in the aggregation of functionalized AuNPs and cause the different red-shifts of the SPR absorption peak and color changes of functionalized AuNPs solutions. The LODs of the colorimetric sensor are 10 ppb/13 ppb/4 ppb by naked eyes and 2.35 ppb/1.68 ppb/0.32 ppb by UV–vis spectroscopy with good linear relationships between the absorbance ratios (A 633nm /A 519nm , A 645nm /A 519nm and A 665nm /A 519nm ) and As 3+ , Cd 2+ and Hg 2+ concentrations, which can be successfully applied to sense and detect As 3+ /Cd 2+ /Hg 2+ pollutants in tap water, pond water, well water, and other domestic water sources. Graphical Abstract
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ANALYST
·
2020
·Vol. 146
·期 4
·1295-1302
·
IF 3.978
Defen Feng,Fucun Wei,Yeyu Wu,Xuecai Tan,Fang Li,Yongkuai Lu,Gaochao Fan,Heyou Han
An ultrasensitive electrochemiluminescence (ECL) biosensor was developed based on MIL-53(Al)@CdS QDs and SiO2@AuNPs for trichlorfon detection. Metal–organic frameworks (MOFs) were used as a loading platform that provided a large surface area to load targets and modified materials onto the electrode. At the same time, SiO2@AuNPs loaded plenty of AuNPs which effectively increased the ECL resonance energy transfer between the CdS QDs, so that the ECL signal was strongly quenched and resulted in an amplified response. In the range of 10−11–10−4 M, the ECL response showed a linear relationship with the concentration (logarithm) of trichlorfon, and the detection limit was 5.1 × 10−12 M (S/N = 3). When the biosensor was applied to detect trichlorfon in lettuce, broccoli, cucumber, and chives, the recoveries obtained from the spiked samples were 97%–105%, 102%–104%, 100%–104%, and 98%–104%, respectively. Thus, this novel ECL biosensor has potential applications for the analysis of trichlorfon in food samples.
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BIOELECTROCHEMISTRY
·
2021
·
IF 5.373
Shengzhong Rong,Lina Zou,Yang Li,Yue Guan,Huilin guan,Ze Zhang,Yingcong Zhang,Hongmin Gao,Hongwei Yu,Fuyang Zhao,Hongzhi Pan,Dong Chang
Early finding and diagnosis are critical for prevention and treatment of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a typical biomarker of HCC. Since AFP level can reflect the severity of HCC, it is essential to ensure the accurate detection of AFP. In this study, through a combination of the advantages exhibited by ordered mesoporous carbon (OMC)@gold nanoparticles (AuNPs) composites and AuPt-methylene blue (AuPt-MB), a disposable ultrasensitive sandwich-configuration electrochemical immunosensor for determination of AFP was designed. Characterized by excellent conductivity, highly ordered pore distribution and great surface area, OMC can be effective in promoting electron transfer and loading a large number of AuNPs. In the meantime, AuNPs can also immobilize AFP-Ab 1 through Au-N bonds. As a new redox-active species, rod-like AuPt-MB demonstrates high conductivity, uniform morphology and excellent biocompatibility, which makes it capable not only to fix AFP-Ab 2 , but also to release electrochemical signals. A wide linearity of 10 fg mL −1 -100 ng mL −1 and a low detection limit of 3.33 fg mL −1 (S/N = 3) were obtained. Moreover, the proposed immunosensor exhibited acceptable selectivity, high stability and reproducibility. The excellent performance in detecting serum samples endows the proposed immunosensor with broad prospects of extensive application in the detection of disease biomarkers.
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