ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Yushen Liu,Juan Wang,Chao Zhao,Xiaoxiao Guo,Xiuling Song,Wei Zhao,Sijie Liu,Kun Xu,Juan Li
Listeria monocytogenes ( L. monocytogenes ) is one of the most common food-borne pathogens . The authors describe a sensitive and reliable multicolorimetric assay for L. monocytogenes using a sensing system based on TMB 2+ etching of gold nanorods . Apt-MNP was used as the capture probe, and IgY-BSA-MnO 2 NPs was chosen as an oxidase-like nano-artificial enzyme to oxidize TMB to generate TMB 2+ . Under the optimized conditions, the longitudinal shift of localized surface plasmon resonances had a linear correlation with the L. m onoc ytogenes concentration in the range between 10 to 10 6 cfu mL −1 . Meanwhile, the sensing system can generate vivid color responses as colorful as a rainbow, and the limit of detection is as low as 10 cfu mL −1 at a glance. Recoveries ranging from 97.4 to 101.3% are found when analyzing spiked food samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of L. monocytogenes .
研究领域:
微生物学
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纳米技术
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食品安全
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 11
·8729-8735
·
IF 3.289
Suyan Wang,Limin Wang,Hongfu Chen,Yulong Wang,Jia Cai,Mingming Yang,Fengquan Liu
Here, a specific anti-Hg2+ monoclonal antibody (mAb) was generated and an eco-friendly immunochromatographic test strip (EFITS) based on a mAb-nanogold probe for rapid and specific detection of Hg2+ in water was developed. In the method, the conjugation of Hg–MNA (6-mercaptonicotinic acid)–BSA (bovine serum albumin) was synthesized as an immunogen, the conjugation of MNA–OVA (ovalbumin) was synthesized and selected as a coating antigen. The specific anti-Hg2+ mAb from BALB/C female mice was screened based on a competitive immunoassay. The coating antigen and goat anti-mouse IgG antibody were coated on a nitrocellulose membrane (NC membrane) as a test line and a control line, respectively. The anti-Hg2+ mAb-nanogold probe was applied to the conjugate pad. Hg2+ competes with OVA–MNA for the mAb-nanogold probe causing a color change on the test line corresponding to the Hg2+ content. Thus, we can distinguish the subtle differences through a strip reader. The resulting EFITS is able to detect Hg2+ with LOD of 0.4 ng mL−1 at 9 min by quantitative analysis. EFITS demonstrated here is eco-friendly (without Hg2+ on the strip), capable of rapid detection, and does not require chelators.
研究领域:
环境科学
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分析化学
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生物技术
·
毒理学
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Qian Wang,Juan Li,Qiuyueming Zhou,Ting He,Zhaoliang Cui,Young Moo Lee,Weihong Xing
Dissolved organic matter (DOM) is recognized as a crucial factor contributing to the irreversible fouling of ultrafiltration membranes. Quartz crystal microbalance with dissipation (QCM-D) offers insights into the kinetics of fouling layer formation and its structure in-situ on a model membrane. However, a clear correlation between the membrane fouling process and the adsorption-desorption results obtained from QCM-D has yet to be established. In this study, piezoelectric β-phase PVDF membranes were prepared, and their fouling behavior in response to three model compounds (humic acid (HA), dextran (DEX), bovine serum albumin (BSA), and their mixed solutions was examined. It was firstly proposed that the spin-coated model PVDF membrane surface exhibited a typical β-phase structure, characterized by regular undulations resulting from strong stretching effects. A novel double layer piezoelectric sensor, consisting of a β-phase layer over a piezoelectric quartz substrate, was designed for QCM-D analysis to assess the mass and stiffness variations of the absorbed DOM layer. The fouling tendency followed the order of BSA > Mixture > DEX > HA, with the foulant monomeric units having a similar molecular mass of around tens of kDa. Irreversible fouling resistance in series model as well as frequency drop in QCM-D were listed as indicators of irreversible fouling caused by foulant-membrane interaction, which realized a well-defined match for the first time.
研究领域:
膜科学
·
环境工程
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
Membranes
·
2021
·Vol. 12
·期 1
·42
·
IF 4.106
Xin Huang,Feng Tian,Guohong Chen,Fanan Wang,Rengui Weng,Beidou Xi
It is of great significance to search for efficient, renewable, biodegradable and economical membrane materials. Herein, we developed an organic-inorganic hybrid regenerated cellulose membrane (ZrO2/BCM) with excellent hydrophilic and anti-fouling properties. The membrane was prepared by introducing ZrO2particles into an N-Methylmorpholine-N-oxide(NMMO)/bamboo cellulose(BC) solution system by the phase inversion method. The physi-chemical structure of the membranes were characterized based on thermal gravimetric analysis (TGA), Fourier transform infrared spectroscopy (ATR-FTIR), field emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD). The modified regenerated cellulose membrane has the excellent rejection of bovine serum albumin (BSA) and anti-fouling performance. The membrane flux of ZrO2/BCM is 321.49 (L/m2·h), and the rejection rate of BSA is 91.2%. Moreover, the membrane flux recovery rate after cleaning with deionized water was 90.6%. This new type of separation membrane prepared with green materials holds broad application potential in water purification and wastewater treatment.
研究领域:
材料科学
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环境工程
·
纳米技术
·
膜技术
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2017
·
IF 6.035
Zhu Xiong,Yun Zhong,Haibo Lin,Fu Liu,Tiantian Li,Jinglong Li
In this work, a feasible and efficient approach was developed to produce Poly(lactic acid (PLA) ultrafiltration membrane with both high permeability and high rejection simultaneously. Poly(L-lactic acid)/Poly(D-lactic acid) (PDLA/PLLA) stereocomplex crystallites were thought to play the critical role different from the traditional pore forming agents or hydrophilic modification. The membrane morphology of the stereocomplex PDLA/PLLA membrane with high porosity and thin skin layer was observed by SEM. The addition of PDLA resulted in the thermodynamic enhancement for phase separation of PLLA casting solution as demonstrated by a rotational rheometer. The PDLA/PLLA stereocomplex crystallization behavior was investigated by DSC and XRD . With increasing the stereocomplex crystallites, the water permeability of PLA membrane significantly increased from ~482 L/m 2 ·h to ~3325 L/m 2 h, while with small loss in degree of rejection. Furthermore, the stereocomplex PLA membrane demonstrated the excellent separation efficiency for nano-TiO 2 and ink dispersion. Besides, the stereocomplex PLA membrane exhibited outstanding anti-protein fouling properties through the static and dynamic BSA antifouling tests. All results demonstrated that the stereocomplexation can effectively tune the pore size evolution and control the permeability, selectivity and fouling resistance.
研究领域:
材料科学
·
膜技术
·
高分子化学
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环境工程
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Feng Yang,Yilan Wang,Huatao He,Guanxiong Wang,Menghan Yang,Meiying Hong,Jin Huang,Yaolei Wang
Water-in-water (W/W) Pickering emulsions , exhibit considerable potential in the food and pharmaceutical fields owing to their compartmentalization and high biocompatibility . However, constrained by the non-uniform distribution of shear forces during emulsification or the spatial obstruction in polydimethylsiloxane (PDMS) passive microfluidic platform , the existing methods cannot generate monodisperse W/W Pickering emulsions with high particle coverage rate, thereby limiting their applications. Herein, a novel microfluidic system is designed for the preparation of monodisperse and highly particle-covered W/W Pickering emulsions under mild conditions. pH-responsive Polyethylene glycol (PEG)/phosphate aqueous two-phase system (ATPS) is used for the emulsions' preparation. Notably, a coverage rate of 96 ± 3 % is obtained by adjusting the length of the helical coiled tube, as well as the size and contact angle of genipin cross-linked BSA (BSA-GP) particles. Moreover, these W/W Pickering emulsions, with surfaces almost completely covered, can maintain monodisperse (N coal = 1.18 ± 0.03) for one day. Furthermore, the results of ranitidine hydrochloride (RH) release demonstrated that the drug release rate of W/W Pickering emulsions in the simulated gastric fluid (SGF) was 10 times faster than that in the neutral solution. We believe that the highly particle-covered monodisperse W/W Pickering emulsions possess great potential applications in bioencapsulation for foods and drug delivery.
研究领域:
化学工程
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材料科学
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药剂学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Xinmeng Zhou,Huan Liu,Zilian Yu,Hao Yu,Decheng Meng,Liran Zhu,Huanjun Li
Hydrogel microneedle patches have emerged as promising platforms for painless, minimally invasive, safe, and portable transdermal drug administration. However, the conventional mold-based fabrication processes and inherent single-functionality of such microneedles present significant hurdles to broader implementation. Herein, we have developed a novel approach utilizing a precursor solution of robust nanocomposite hydrogels to formulate photo-printable inks suitable for the direct 3D printing of high-precision, triple-responsive hydrogel microneedle patches through digital light processing (DLP) technology. The ink formulation comprises four functionally diverse monomers including 2-(dimethylamino)ethyl methacrylate , N -isopropylacrylamide, acrylic acid , and acrylamide , which were crosslinked by aluminum hydroxide nanoparticles (AH NPs) acting as both reinforcing agents and crosslinking centers. This results in the formation of a nanocomposite hydrogel characterized by exceptional mechanical strength , an essential attribute for the 3D printing of hydrogel microneeedle patches. Furthermore, this innovative 3D printing strategy facilitates facile customization of microneedle geometry and patch dimensions. As a proof-of-concept, we employed the fabricated hydrogel microneedles for transdermal delivery of bovine serum albumin (BSA). Importantly, these hydrogel microneedles displayed no cytotoxic effects and exhibited triple sensitivity to pH, temperature and glucose levels, thereby enabling more precise on-demand drug delivery. This study provides a universal method for the rapid fabrication of hydrogel microneedles with smart responsiveness for transdermal drug delivery applications.
研究领域:
材料科学
·
生物医学工程
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药学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2024
·
IF 5.1
Gang Yu,Jianxin He,Zhongshan Gao,Linglin Fu,Qiaozhi Zhang
Advanced glycation end-products (AGEs) are complex and heterogeneous compounds widely present in processed foods. Previous studies evidenced the adverse effects of AGEs on gut homeostasis, but the precise pathological mechanisms and molecular pathways responsible for the disruption of intestinal barrier integrity by AGEs remain incompletely elucidated. In this study, protein-bound AGEs (BSA-MGO), the most common type of dietary AGEs, were prepared by methylglyoxal-mediated glycation, and an in vitro human epithelial colorectal adenocarcinoma (Caco-2) cell model was employed to evaluate the impact of protein-bound AGEs on gut epithelial function. Results showed that exposure to BSA-MGO significantly increased the permeability of Caco-2 cell monolayers as evidenced by the decreased transepithelial electrical resistance value, increased paracellular transport of FITC-dextran, and down-regulated tight-junction proteins. Parallelly, BSA-MGO induced pro-inflammatory responses and oxidative stress in the monolayers. Transcriptomic profiling further revealed that BSA-MGO disrupted the retinol metabolism, thereby contributing to the barrier integrity damage in epithelial cells. Overall, these results provide valuable insights into the disrupting effects of dietary AGEs on intestinal barrier function, and the perturbed pathways present potential targets for further exploration of the molecular mechanisms underlying the detrimental effect of processed foods on gut health.
研究领域:
胃肠病学
·
细胞生物学
·
分子生物学
·
营养学
·
炎症
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Dan Yang,Yao Zhou,Mengqi Wang,Ying Yuan,Yuqi Feng,Yapeng Fang,Guoliang Li,Dechun Liu,Xiaolin Yao
Biomacromolecules in physiological environments would adsorb onto the nanoparticles (NPs) to form corona layers, in which protein coronas (PCs) are the major constituent. PCs always play diverse influences on the fate of NPs in vitro and in vivo, especially for active-targeting NPs (e.g., transferrin-modified nanoparticles, Tf-NP). In order to eliminate the inhibition of PCs on the efficiency of Tf-NP, the precoated Tf-NP with bovine serum albumin (BSA, B@Tf-NP) was designed to fabricate an “active PCs” (PCs formed by artificial modification) against the “passive PCs” (PCs formed in the biological environments), which was inspired by the formation pattern of PCs. The results indicated that B@Tf-NP had similar particle size, dispersion, and physical stability with Tf-NP. Surprisingly, B@Tf-NP enhanced the cellular uptake in enterocytes and permeability in intestinal tract of mice. Notably, the concentration ratio of BSA to Tf that could ensure Tf revealed timely during the interacted process was considered to be appropriate. These findings provide an easy while efficient design platform for active-targeting NPs to overcome the biomacromolecular barrier in oral administration.
研究领域:
纳米技术
·
药学
·
生物医学工程
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Xinying Yuan,Xue Zhang,Qionghua Zhang,Gang Zhao
Three forms of cobalt, including cobalt chloride (CoCl 2 ), cobalt (II) acetylacetonate (Co(acac) 2 ) and cobalt (III) acetylacetonate (Co(acac) 3 ), were evaluated for their interactions with bovine serum albumin (BSA). Results reveal distinct binding mechanisms among cobalt compounds. CoCl 2 bound to negatively charged residues on the BSA surface via electrostatic interactions, exhibiting weak binding affinity ( K b = (0.09–2.3) × 10 3 L·mol −1 ) and high temperature sensitivity (Δ G ° decreased significantly with increasing temperature). Co(acac) 2 partially penetrated the hydrophobic microenvironment of BSA through its hydrophobic ligands, achieving moderate binding strength ( K b = (3.24–6.84) × 10 3 L·mol −1 ) with synergistic contributions from hydrogen bonding and van der Waals forces (Δ H ° = −48.80 ± 0.01 kJ·mol −1 , Δ S ° = −89.87 ± 0.01 J·mol −1 ·K −1 ). Co(acac) 3 demonstrated the strongest binding capacity ( K b = (9.27–32.3) × 10 4 L·mol −1 ), attributed to multimodal interactions (hydrophobic embedding, coordination bonds, and hydrogen bonding) facilitated by its three hydrophobic ligands. The binding affinity of cobalt compounds to BSA increases with ligand complexity and hydrophobicity (CoCl 2 → Co(acac) 2 → Co(acac) 3 ). UV–Vis, three-dimensional fluorescence, and circular dichroism spectra further revealed that organic acetylacetonate ligands enhanced BSA structure perturbation, intensifying with ligand number. Co(acac) 3 induced significant conformational changes in BSA (19.98 % reduction in α -helix content), increased microenvironmental polarity (5 nm fluorescence redshift), and optimized spectral overlap between donor and acceptor (34.6 % increase in J value) by shortening the binding distance ( r = 2.83 ± 0.01 nm), thereby elevating energy transfer efficiency ( E = 34.65 %). This study provides molecular-level insights into the biocompatibility and toxicity assessment of cobalt-based compounds.
研究领域:
无机化学
·
生物无机化学
·
分子毒理学
·
物理化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Qiang Xue,Kaisong Zhang
Current membranes have limitations in permeability and selectivity, and require long-time stability, which drive the development of high-performance nanofiltration (NF) membranes. MXene is a new type of highly hydrophilic and negatively charged two-dimensional (2D) nanosheets material, especially its supernatant has good dispersibility and stability. The MXene supernatant containing several layers was treated as aqueous solvent for interfacial polymerization, consequently the prepared MXene nanocomposite membrane could maintain high permeselectivity even under low pressures required for low carbon. The characterization results showed that the MXene nanocomposite membrane had better hydrophilicity and higher negative charge. At the same time, the Na 2 SO 4 rejection was increased to 97.6% and the pure water flux was improved by about 1.7 times compared with the pristine membrane. In the BSA antifouling test and 58-day persistent desalination test, the MXene nanocomposite membrane showed the better antifouling property, high rejection and good stability. As an excellent 2D modifier, MXene provided fresh ideas and guidance for low-carbon and persistent NF membranes with excellent separation performance.
研究领域:
材料科学
·
环境工程
·
纳米技术
·
水处理
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Wentang Zhao,Mei Wu,Lisha Mei,Hongling Li
An ultra-sensitive electrochemical immunosensor for detecting carcinoembryonic antigen was prepared. Graphene oxide-gold was used as the substrate modifier of the sandwich immunosensor. The high conductivity of graphene oxide effectively enhanced the electron transfer rate. The large specific surface area of graphene and the biocompatibility of gold nanoparticles increased the number of primary antibodies. The bovine serum albumin coated silver – platinum composite was used as a secondary antibody tag to prepare signal probes. The excellent biocompatibility of bovine serum albumin increased the number of secondary antibody connections. Silver nanoparticles increased the speed of electron transfer, and platinum further enhanced electrochemical sensing capabilities by catalyzing hydrogen peroxide. The immunosensor showed good performance for detecting carcinoembryonic antigen. Under the optimal conditions, the dynamic response range for carcinoembryonic antigen was from 0.005 ng·mL−1 to 100 ng·mL−1 with a detection limit of 0.76 pg·mL−1. The immunosensor also shown high selectivity, suitable stability, and good reproducibility, which provides new capabilities for clinical carcinoembryonic antigen detection.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）