Nano Research
·
2020
·Vol. 14
·期 3
·879-886
·
IF 8.183
Zhao Shen Fei,Hu Fang Xin,Shi Zhuan Zhuan,Fu Jing Jing,Chen Yue,Dai Fang Yin,Guo Chun Xian,Li Chang Ming
It is critical for fabricating flexible biosensors with both high sensitivity and good selectivity to realize real-time monitoring superoxide anion (O 2 •− ), a specific reactive oxygen species that plays critical roles in various biological processes. This work delicately designs a Mn 3 (PO 4 ) 2 /MXene heterostructured biomimetic enzyme by assembling two-dimensional (2-D) Mn 3 (PO 4 ) 2 nanosheets with biomimetic activity and 2-D MXene nanosheets with high conductivity and abundant functional groups. The 2-D nature of the two components with strong interfacial interaction synergistically enables the heterostructure an excellent flexibility with retained 100% of the response when to reach a bending angle up to 180°, and 96% of the response after 100 bending/relaxing cycles. It is found that the surface charge state of the heterostructure promotes the adsorption of O 2 •− , while the high-energy active site improves electrochemical oxidation of O 2 •− . The Mn 3 (PO 4 ) 2 /MXene as a sensing platform towards O 2 •− achieves a high sensitivity of 64.93 µA·µM −1 ·cm −2 , a wide detection range of 5.75 nM to 25.93 µM, and a low detection limit of 1.63 nM. Finally, the flexible heterostructured sensing platform realizes real-time monitoring of O 2 •− in live cell assays, offering a promising flexible biosensor towards exploring various biological processes.
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JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2017
·
IF 0.697
Yanni Miao,Qi Liu,Wei Wang,Li Liu,Li Wang
A new chiral capillary electrophoresis (CE) analytical method for the determination of six pairs of amino acid enantiomer-derivatized precapillary with 9-fluorenylmethoxycarbonyl chloride (FMOC) was developed. CE separation parameters such as the pH of background electrolyte, the boric acid concentration, the addition of D-fructose and isopropanol, and the effect of new binary chiral selectors on the electropherograms were investigated. Precapillary and in-capillary derivatization was compared as well. The results showed that enantiomeric separations were obtained with enantio-resolution (Rs) ranged from 2.61 to 9.99 for the FMOC-derivatized amino acid enantiomers except alanine with Rs 1.06. Precapillary derivatization method provides overall better Rs for nearly all the targets except FMOC-aspartate with efficiency up to 1.309 × 106 plates and limit of detection (LOD) down to 2.5 μM. This method was successfully applied to analyze the concentration of the D/L-glutamic acid and D/L-aspartate in seven rice wine samples.
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Cell Communication and Signaling
·
2025
·Vol. 23
·期 1
·1-21
·
IF 8.9
He Qiongyao,He Wu,Ren Yanlin,Wu Wenbin,Dong Hui,Yuan Gang,Ren Huihui,Wang Xinwei,Lu Fuer,Wang Dingkun
Ferroptosis, an iron-dependent form of programmed cell death, is closely associated with tubular damage in diabetic nephropathy (DN). Glutathione peroxidase 4 (GPX4) is an important anti-oxidant enzyme, and plays a crucial role in protecting against ferroptosis. However, the regulatory mechanism of GPX4 expression levels in renal tubular epithelial cells (RTECs) remains elusive. This study reveals that ferroptosis occurs in the late-stage of DN, and the GPX4 level is significantly downregulated in DN patients, animal models and cell models. By applying database predictions, luciferase reporter assays and chromatin immunoprecipitation, we find that vitamin D receptor (VDR) transcription factor promotes GPX4 expression and plays a key role in inhibiting ferroptosis of RTECs. VDR knockout exacerbates ferroptosis in RTECs and worsens renal function, while intraperitoneal injection of VDR agonist paricalcitol significantly improves renal injury. Proteomics analysis suggests that E3 ligase PRPF19 mediates ubiquitination degradation of VDR and is an important therapeutic target for DN. Therefore, through molecular docking, targeted fishing technology using high-performance affinity beads, and surface plasmon resonance (SPR), we screen and identify berberine (BBR) as a novel inhibitor of PRPF19, which offers renal protection by inhibiting VDR degradation and tubular ferroptosis. These findings elucidate the role of ferroptosis in DN renal tubular injury, and suggest that PRPF19 is a promising therapeutic target.
研究领域:
医学
·
分子生物学
·
肾脏病学
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高影响因子
Molecular Medicine Reports
·
2021
·Vol. 24
·期 4
·1-10
·
IF 2.952
Ruiying Liu,Jing Meng,Danfei Lou
The aim of the present study was to examine whether adiponectin could inhibit cardiomyocyte senescence induced by D‑galactose (D‑gal), and whether it functioned via the adiponectin receptor 1 (AdipoR1)/adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) signaling pathway. For this purpose, the expression levels of adiponectin, AdipoR1 and APPL1 in mouse plasma and myocardial tissues were detected via reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting. An adiponectin‑overexpression plasmid was transfected into D‑gal‑treated H9c2 cells prior to the detection of AdipoR1 and APPL1 expression by RT‑qPCR. Senescence‑associated β‑galactose staining was then performed to observe cellular senescence following the transfection of small interfering RNAs (si) targeting AdipoR1 and APPL1 into D‑gal‑treated H9c2 cells overexpressing adiponectin. Commercial kits were used to detect reactive oxygen species (ROS) production and malondialdehyde (MDA) content in the different groups. The expression levels of heme oxygenase (HO)‑1 and high mobility group box 1 (HMGB1) were examined by western blot analysis. The results revealed that the expression levels of adiponectin, AdipoR1 and APPL1 were downregulated in aged mouse plasma, myocardial tissues and D‑gal‑treated cardiomyocytes. It was also observed that AdipoR1 and APPL1 expression levels were significantly upregulated following the overexpression of adiponectin into D‑gal‑treated cardiomyocytes. Moreover, adiponectin overexpression reduced cellular senescence induced by D‑gal and the expression of p16 and p21; these effects were reversed following transfection with si‑AdipoR1 and si‑APPL1. Adiponectin also downregulated the levels of ROS and MDA in D‑gal‑treated H9c2 cells via AdipoR1/APPL1. Additionally, the release of HO‑11/HMGB1 was affected by adiponectin via AdipoR1/APPL1, and adiponectin/AdipoR1/APPL1 suppressed ROS production via HO‑1/HMGB1. On the whole, the present study demonstrated that adiponectin played an inhibitory role in cardiomyocyte senescence via the AdioR1/APPL1 signaling pathway and inhibited the levels of oxidative stress in senescent cardiomyocytes via the HO‑1/HMGB1 signaling pathway.
研究领域:
心脏病学
·
细胞生物学
·
分子生物学
·
药理学
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Nature Communications
·
2026
·
IF 18.1
Li Yang,Zhang Yuanming,Zeng Zhaojian,Chen Yong,Xu Xiaoming,Zou Zhigang,Li Zhaosheng
The hydrogen spillover effect embodies a sophisticated and multifaceted phenomenon for H atom equivalents. Despite substantial efforts to identify potential candidates, including d -block metals like Ru and Pd, fundamental aspects of dynamic interplay of activation and spillover processes are still inadequately understood, hindering efficient hydrogenation reactions and high-capacity chemical hydrogen storage. Here, we quantify a comprehensive platform of thermodynamic‒kinetic descriptors to systematically elucidate the full scope of the hydrogen spillover pathway and transcend the limitations of conventional approaches that focus solely on the surface concentration of spilled-over hydrogen, shielding the signal heterogeneity inherent in zones of differential hydrogen availability. Taking a well-defined d -block M/TiO 2− x (M = Ru, Mn, Fe, Co, or Ni) as a representative case by combining spectroscopic quantification and computational investigation, the nonlinear spillover capability and rate of d -block metal originates from the synergistic overlap of its unoccupied d - s orbitals, and the σ and σ* orbitals of the H atom equivalents interact to form a weak antibonding state, reducing the dissociation of the H–H bond and M–H bond. This methodology provides a refined lens for dissecting spillover mechanisms, facilitating a profound mechanistic and spatial understanding of rational metal dilution.
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高影响因子
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
·
2020
·Vol. 59
·期 24
·9414-9417
·
IF 12.959
Shuai Liu,Xiaomeng Liu,Sangen Zhao,Youchao Liu,Lina Li,Qingran Ding,Yanqiang Li,Zheshuai Lin,Junhua Luo,Maochun Hong
Birefringent materials, which can modulate the polarization of light, are almost exclusively limited to oxides. Peroxides have long been overlooked as birefringent materials, because they are usually not stable in air. Now, the first peroxide birefringent material Rb 2 VO(O 2 ) 2 F is reported, the single crystals of which keep transparency after being exposed in the air for two weeks. Interestingly, Rb 2 VO(O 2 ) 2 F does not feature an optimal anisotropic structure, but its birefringence (Δn=0.189 at 546 nm) exceeds those of the majority of oxides. According to the first-principles calculations, this exceptional birefringence should be attributed to the strong electronic interactions between localized π orbital of O 2 2− anions and V 5+ 3d orbitals, which may be also favorable to the stability in the air for Rb 2 VO(O 2 ) 2 F. These findings distinguish peroxides as a brand-new class of birefringent materials that may possess birefringence superior to the traditional oxides. Graphical Birefringent materials , which can modulate the polarization of light, are almost exclusively limited to oxides. Now, a peroxide birefringent material, Rb 2 VO(O 2 ) 2 F, is reported. Single crystals retain transparency after being exposed in the air for two weeks. Rb 2 VO(O 2 ) 2 F does not feature an optimal anisotropic structure, but its birefringence (Δ n =0.189 at 546 nm) exceeds those of the majority of oxides.
研究领域:
材料科学
·
光学材料
·
无机化学
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固态物理
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CATALYSIS LETTERS
·
2026
·Vol. 156
·期 4
·95
·
IF 2.5
Tang Zhanqiu,Fu Yuqing,Feng Xinyi,Wu Zhenglong,Qin Weidong
Enantioselective catalytic oxidation of d -glucose has been extensively applied in food, pharmaceutical, and chemical industries. In this work, a nitrogen-rich graphitic carbon nitride ( g- C 3 N 5 ) was synthesized through the pyrolysis of 3-amino-1,2,4-triazole. This material exhibited a reduced band gap, enhanced electrical conductivity, and superior photocatalytic performance compared to graphitic C 3 N 4 ( g- C 3 N 4 ), facilitating more efficient photocatalytic oxidation of glucose with higher yield. The surface of g- C 3 N 5 was subsequently capped with a molecularly imprinted polymer (MIP) layer by employing d -glucose as the template molecule, and 4-vinylphenylboronic acid as the functional monomer. The resulting g- C 3 N 5 -MIPs demonstrated the ability to selectively photocatalyze the oxidation of d -glucose, producing d -glu acid and H 2 O 2 . Notably, the relative selectivity coefficient for d -glucose over l -glucose increased by a factor of 8.79 compared to the non-imprinted g- C 3 N 5 . This work presents an effective approach for synthesizing advanced g-C 3 N 5 -MIP-based photocatalysts, and highlights their potential for highly enantioselective aerobic oxidation of d -glucose. Graphical
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Shuang Li,Shuting Li,Longda Li,Zhaoquan Li,Yixi Li,Linhong He,Yanying Liu,Fan Yang,Yong Huang,Xinchun Li
Ion channels can selectively recognize and transport biological species via an exquisite protein conformational switch. Classically, glucose-specific ion channels strictly transport the disomer over the lisomer. Inspired by this, we report a biomimetic d-glucose-responsive ion gate. We designed and synthesized a functional receptor, a chiral biphenylboronic acid (cBBA) derivative, which was used as a responsive element in an artificial nanopore. Due to its high binding affinity for d-glucose molecules, the biomimetic ion gate exhibits a sensitive and specific gating effect in response to d-glucose stimulation, thereby modulating the ionic current rectification (ICR) behavior. Remarkably, an extremely low concentration (e.g., 10 pM) of d-glucose stimulus could generate a remarkable ionic current change. After binding of physiological concentration of d-glucose (e.g., 5 mM), the ionic current modulation drastically decreased, with the switch ratio reaching up to 1530, showing a powerful gating capacity. Particularly, other saccharide analogues or the l-glucose isomer failed to activate the ion gate. Moreover, this gating effect was pH-dependent and demonstrated reversible ON-OFF ability when altering the medium acidity. Density functional theory (DFT) calculations unraveled that the designed artificial receptor can preferentially bind d-glucose over the l-glucose enantiomer, and finite element simulations revealed the ion gating mechanism in the d-glucose sensing event. The nanopore sensor was successfully applied to the electrochemical assay of glucose in biofluids.
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Food Bioscience
·
2021
·
IF 4.24
Feier Cheng,Xinhui Ge,Yao Zhang,Jia Li,Shaoqing Yan,Yunlong Li,Min Wang
The hypoglycemic effects and underlying molecular mechanisms of combining two dietary compounds, quercetin (Que) and d-chiro-inositol (DCI) on hepatic insulin resistance in C57BL/6J mice that were fed a high fat diet and saturated palmitic acid (PA)-treated hepatocytes were studied. Catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) are important antioxidant enzymes . Insufficient secretion of these enzymes will inhibit the normal phosphorylation of insulin receptors in the liver, thereby limiting the transmission of insulin signals and producing insulin resistance. The results showed that QD (both Que and DCI) led to the recovery of the activity of CAT, SOD, GPx, suggesting that QD was helpful in preventing mice hepatic insulin resistance. In PA-induced human hepatoma cells (HepG2), QD improved the antioxidant level in cells. The fluoro-immunoassay showed that QD effectively reduced the translocation of protein kinase Cε to cell membranes in the HepG2. Western blotting results suggested that QD increased the signal transduction in the IRS-2/PI3K/Akt/FoxO1 pathway. Overall, these results showed that the combination of 5 μmol Que and 5 μmol DCI ameliorated high fat diet-induced hepatic insulin resistance.
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Materials Today Communications
·
2019
·
IF 1.859
Shaogang Liu,Libo Wang,Xiaolong Wang,Lu Liu,Aiguo Zhou,Xinxin Cao
In this study, Ti 3 C 2 was delaminated into 2D single or few monolayers-thick nanomaterials (d-Ti 3 C 2 ) by DMSO intercalation and sonication method. Then, d-Ti 3 C 2 /polyvinyl alcohol (PVA) films were prepared by solution casting process. The structures, thermal stabilities, thermal behaviors, and surface properties of the resulting materials were characterized by X-ray diffractometry (XRD), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR). Furthermore, the experimental results showed improvements in mechanical and thermal characteristics of d-Ti 3 C 2 /PVA films, as compared to those of pure PVA. The addition of d-Ti 3 C 2 had beneficial effects on the tensile strength , elongation at break, modulus of elasticity , thermal stability, and crystallinity of PVA matrix. With 0.5 wt% of d-Ti 3 C 2 , d-Ti 3 C 2 /PVA composite exhibited the best comprehensive properties.
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MOLECULES
·
2019
·Vol. 24
·期 16
·2972
·
IF 3.06
Jiang-Zhen Qiu,Long-Fei Wang,Jiuxing Jiang
Two-dimensional aluminophosphate is an important precursor of phosphate-based zeolites; a new Sun Yat-sen University No. 6 (SYSU-6) with |Hada|2[Al2(HPO4)(PO4)2] has been synthesized in the hydrothermal synthesis with organic structure-directing agent (OSDA) ofN,N,3,5-tetramethyladamantan-1-amine. In this paper, SYSU-6 is characterized by single-crystal/powder X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray analysis, transmission electron microscopy, infrared and UV Raman spectroscopy, solid-state27Al,31P and13C magic angle spinning (MAS) NMR spectra, and elemental analysis. The single-crystal X-ray diffraction structure indicates that SYSU-6 crystallized in the space groupP21/n, with a = 8.4119(3), b = 36.9876(12), c = 12.5674(3), α = 90°, β = 108.6770(10)°, γ = 90°, V = 3704.3(2) Å3, Z = 4, R = 5.12%, for 8515 observed data (I > 2σ(I)). The structure has a new 4,12-ring layer framework topology linked by alternating AlO4and PO4tetrahedra. The organic molecules reside between the layers and are hydrogen-bonded to the inorganic framework. The new type of layer provides a greater opportunity to construct zeolite with novel topology.Keywords:aluminophosphate;2-D zeolite;SYSU-6
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ENVIRONMENTAL TOXICOLOGY
·
2025
·
IF 3.2
Yang Li,Keyuan Zhong,Sijie Zhang,Shiyi Duan,Kaijie Huang,Xiaofang Che,Xinchun Guo,Huiqiang Lu
D-tetramethrin is a common insecticide that is important for the control of mosquito-transmitted diseases such as malaria and dengue and, due to its widespread use, it is frequently detected in environmental systems. However, there is currently very little information on the influence of D-tetramethrin on non-target organisms. In this study, zebrafish embryos were exposed to various concentrations of D-tetramethrin (0, 3, 6, and 9 mg/L) from 6 to 72 h past fertilization (hpf) to ascertain the influence of D-tetramethrin on the zebrafish immune system and development. We found that D-tetramethrin exposure led to a significant decrease in heart rate, an increase in the yolk area and tail flick frequency, and a shortening of body length in zebrafish larvae, compared with the control group. The number of macrophages and neutrophils in the experimental group of zebrafish larvae decreased significantly and the oxidative stress levels increased compared with the control group. The malondialdehyde (MDA) content and reactive oxygen species (ROS) content increased significantly, while catalase (CAT) activity increased and superoxide dismutase (SOD) activity decreased in the experimental group. At the same time, apoptosis increased in the zebrafish embryo cells, and the expression of apoptosis-related genes such as p53 , Bax , and Bcl-2 was abnormal. Moreover, the pro-inflammatory genes IL-8 , IFN-γ , IL-6 , and TNF-α were up-regulated, while the anti-inflammatory gene, IL-10 , was down-regulated. Therefore, D-tetramethrin significantly affects the immune system and oxidative stress levels in zebrafish, inducing cell apoptosis and negatively affecting embryo development. These results provide novel data for the toxicity of D-tetramethrin and the potential adverse effects of environment residues.
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