APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Yujie Qiang,Shengtao Zhang,Liping Wang
Deoxyribonucleic acid (DNA) was investigated for suppressing copper corrosion in 0.5 M H 2 SO 4 through electrochemical measurement, microscopic surface observation, X-ray photoelectron spectroscopy (XPS) analysis and molecular dynamics (MD) simulation. Results indicate that DNA acts as an effective cathodic-type inhibitor that mainly suppresses cathodic reaction by forming anchored DNA-adsorption film. The film shows active blocking effect, obeys the Langmuir adsorption model and involves physisorption and chemisorption . The MD simulation gives the new insights at molecular level and indicates the formation of an interesting double barrier of DNA molecule on the Cu (111) surface due to its unique twist structure.
研究领域:
材料科学
·
电化学
·
表面科学
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DOI
高影响因子
Journal of Radiation Research and Applied Sciences
·
2020
·
IF 1.804
Xue-Wen Liu,Ning-Yi Liu,Yuan-Qing Deng,Shan Wang,Ting Liu,Yu-Cai Tang,Yuan-Dao Chen,Ji-Lin Lu
ABSTRACT In recent years, ruthenium (II) complexes have attracted attention due to high DNA topoisomerase I inhibitory activities and good anticancer activities. Herein, an asymmetric ligand 2-(5-(1, 10-phenanthroline))-1 H-4,5-diphenyl-imidazole (pdpi) and its ruthenium complexes were synthesized. DNA-binding mode of two complexes was determined as intercalation by using UV–vis spectra, emission spectra, viscosity, and molecular docking experiments. DNA photocleavage experimental results show that two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they both displayed good topo I inhibition activities. The cytotoxic activities results show that complex 2 displays better antitumor activities against Eca-109 cells and A549 cells (IC 50 = 25.97 ± 3.33 μM and 28.83 ± 2.49 μM, respectively) compared to complex 1 .
研究领域:
化学
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药理学
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癌症研究
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Biyan Xu,Delun Zheng,Weiwei Qiu,Feng Gao,Shaoxiong Jiang,Qingxiang Wang
In this work, a novel electrochemical DNA biosensor has been developed based on the hybrid film of fern leaf-like α-Fe 2 O 3 microparticles and chitosan (CS). The fern leaf-like α-Fe 2 O 3 microparticles were synthesized via a facile template-free hydrothermal method, and their morphologies were characterized by X-ray diffraction, energy dispersive spectrometry, scanning electron microscope, and transmission electron microscope. Electrochemical characterization assays revealed that the hybrid film modified electrode had remarkable synergistic effects of the large accessible surface area and high electrical conductivity of semiconductive Fe 2 O 3 , and the good film stability of CS. Based on the rich amino groups on CS, the CS–Fe 2 O 3 hybrid film was employed as a functional matrix for probe DNA immobilization using terephthalaldehyde (TPA) as a bifunctional arm-linker. The hybridization capacity of the developed biosensor was evaluated with electrochemical impedance spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− as the indicating probe. A wide dynamic detection range from 1.0×10 −14 to 1.0×10 −10 M with ultralow detection limit of 5.6×10 −15 M was achieved for the target DNA. The hybridization selectivity experiments further revealed that the biosensor could discriminate fully complementary sequences from one-base mismatched, three-base mismatched, and non-complementary sequences. Moreover, the biosensor showed the advantage of good regeneration ability and reproducibility.
研究领域:
生物化学
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电化学
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纳米技术
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生物传感器
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材料科学
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产品关联
DOI
PubMed
高影响因子
International Journal of Clinical and Experimental Pathology
·
2015
·
IF 1.891
Shupeng Zhao,Haigang Chang,Pengju Ma,Guojun Gao,Cailing Jin,Xinli Zhao,Wenke Zhou,Baozhe Jin
Objective: To investigate the effect of isoliquiritigenin on the activity of DNA topoisomerase (TOP I) and its inhibitory effect on the growth of U87 glioma cells. Methods: This study investigated the inhibitory effect of isoliquiritigenin on the growth of U87 glioma cells and its cytotoxicity by MTT method and determined the effect of isoliquiritigenin on TOP I activity by agarose gel electrophoresis. On this basis, we studied the interaction between isoliquiritigenin and TOP I and DNA. Finally, we further discussed the effect of isoliquiritigenin on the activity of Caspase 3, the apoptosis protein of U87 glioma cells. Results: Isoliquiritigenin could inhibit the growth of U87 glioma cells (half inhibitory concentration IC50: 0.221 mM) and is of low cytotoxicity to normal cells. Agarose gel electrophoresis showed that isoliquiritigenin had significant inhibitory effect on TOP I activity. Molecular simulation results indicated that isoliquiritigenin took priority of binding to the active center of TOP I, and formed hydrogen bonds with the catalytic site Try723. Finally, Caspase 3 activity detection results suggested that isoliquiritigenin could significantly increase the activity of Caspase 3 (P < 0.05). Conclusion: Isoliquiritigenin had a reversible inhibitory effect on TOP I activity, reduced the rate of single strand DNA unwinding in tumor cells, and thus played an important role in inducing the apoptosis of U87 glioma cells.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2023
·Vol. 29
·期 57
·e202301968
·
IF 4.3
Zhuo Zhang,Ting Liu,Yawen Li,Yang Yu,Lin Ning,Xuefan Qiu,Chencan Li,Ling Wang,Wenlong Xu
Graphical We demonstrate the temporal programming of the assembly and disassembly of EuW 10 nanospheres using a pH clock reaction of acid/urease hydrolysis. The pH-responsive C 12 DMAO endow EuW 10 nanospheres with dynamical properties, in which fluorescence changes are coded in this system. These fluorescent nanospheres provide new application in time-programmed information encryption. Self-assembly exploits noncovalent interaction to offer an effective method for the fabrication of materials. For Na 9 [EuW 10 O 36 ] ⋅ 32H 2 O (EuW 10 ), the negative charges and abundant oxygen atoms on its surface provide a handle for static self-assembly. New properties are envisioned for EuW 10 aggregates which are able to display such kinetics and time-programming characteristics, in order to satisfy more complex and intelligent application scenarios, such as DNA binding and information encryption. In this work, EuW 10 coupling with stimuli-responsive dodecyl dimethylamine oxide (C 12 DMAO) can generate versatile aggregates with pH-responsive properties. We demonstrated the temporal programming of the assembly and disassembly of EuW 10 nanospheres using a pH clock reaction of acid/urease hydrolysis. The pH clock reaction endows EuW 10 assemblies with dynamical properties, in which the charges and fluorescence changes are coded in this system. These fluorescent assemblies provide new application in time-programmed DNA capture and information encryption.
研究领域:
材料科学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Fei Qu,Zeqiu Chen,Jinmao You,Cuihua Song
Human telomere DNA plays a vital role in genome integrity control and carcinogenesis as an indication for extensive cell proliferation. Herein, silver nanoclusters (Ag NCs) templated by polymer and unmodified gold nanoparticles (Au NPs) are designed as a new colorimetric platform for sensitively differentiating telomere DNA with different lengths, monitoring G-quadruplex and dsDNA. Ag NCs can produce the aggregation of Au NPs, so the color of Au NPs changes to blue and the absorption peak moves to 700 nm. While the telomere DNA can protect Au NPs from aggregation, the color turns to red again and the absorption band blue shift. Benefiting from the obvious color change, we can differentiate the length of telomere DNA by naked eyes. As the length of telomere DNA is longer, the variation of color becomes more noticeable. The detection limits of telomere DNA containing 10, 22, 40, 64 bases are estimated to be 1.41, 1.21, 0.23 and 0.22 nM, respectively. On the other hand, when telomere DNA forms G-quadruplex in the presence of K + , or dsDNA with complementary sequence, both G-quadruplex and dsDNA can protect Au NPs better than the unfolded telomere DNA. Hence, a new colorimetric platform for monitoring structure conversion of DNA is established by Ag NCs-Au NPs system, and to prove this type of application, a selective K + sensor is developed.
研究领域:
生物化学
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纳米医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jiawen Han,Xujuan Lv,Yuwei Zhang,Juan Wang,Daoqing Fan,Shaojun Dong
DNA logic nanodevices are powerful tools for both molecular computing tasks and smart bioanalytical applications. Nevertheless, the hour-level operation time and high cost caused by the frequent redesign/reconstruction of gates, tedious strand-displacement reaction, and expensive labeled probes (or tool enzymes) in previous works are ineluctable drawbacks. Herein, we report an ultrafast and cost-effective system for engineering concurrent DNA logic nanodevices (CDLNs) by combining polythymine CuNCs with SYBR Green I (SG I) as universal dual-output producers. Particularly, benefiting from the concomitant minute-level quick response of both unlabeled illuminators and the exquisite strand-displacement-free design, all CDLNs including contrary logic pairs (YES∧NOT, OR∧NOR, and Even∧Odd number classifier), noncontrary ones (IDE∧IMP, OR∧NAND), and concatenated circuits are implemented in just 10 min via a “one-stone-two-birds” method, resulting in only 1/12 the operation time and 1/4 the cost needed in previous works, respectively. Moreover, all of them share the same threshold value, and the dual output can be easily visualized by the naked eye under a portable UV lamp, indicating the universality and practicality of this system. Furthermore, by exploiting the “positive/negative cross-verification” advantages of concurrent contrary logic, the smart in vitro analysis of the polyadenine strand and its polymerase is realized, providing novel molecular tools for the early diagnosis of cancer-related diseases.
研究领域:
生物化学
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分子生物学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ruyue Cong,Kaiyue Hu,Dandan Hu,Jingran Sun,Jinglu Yao,Zhiyong Guo,Sui Wang,Yufang Hu
The detection of UDG activity is essential for understanding DNA damage repair mechanisms, assessing cellular genetic stability, and advancing diagnostics and treatments for related diseases. An advanced electrochemical biosensor based on a silver-enhanced copper metal–organic framework (Ag@Cu-MOF) has been developed for the sensitive and selective detection of uracil-DNA glycosylase (UDG) activity in cellular environments. This design incorporates a prism substrate to stabilize the liquid phase on the electrode surface, with DNA probes immobilized on the prism surface. Upon UDG-catalyzed uracil excision, the probe undergoes structural changes, initiating a cascade reaction. The abundance of free DNA ends facilitates the approach of MOF materials to the electrode interface, creating a stable environment conducive to H 2 O 2 reduction, thereby amplifying the electrochemical signal. Comparative catalytic analyses revealed that the Ag@Cu-MOF-modified electrode, with its enhanced conductivity and high surface area, achieved efficient catalysis compared to Cu-MOF. The biosensor exhibited a linear response to UDG concentrations ranging from 0.00002 to 0.5 U/mL, with an impressively low detection limit of 0.00002 U/mL, highlighting its capability for quantitative analysis. The sensor demonstrated high selectivity, minimal interference from other biomolecules, and stable performance across multiple cycles, underscoring its robustness. Furthermore, UDG activity was successfully detected at varying cell densities and under hypoxic conditions, providing valuable insights into cellular DNA repair mechanisms and responses to stress. In summary, the Ag@Cu-MOF-based biosensor offers a promising platform for detecting UDG activity in cancer diagnostics and cellular research, with potential applications in monitoring DNA repair activities under both physiological and pathological conditions.
研究领域:
生物化学
·
分子生物学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
·Vol. 80
·期 7
·1274-1282
·
IF 1.1
Guan Zhimin,Xie Yaoyao,Li Ke,An Junling,Wang Ping
A ratiometric electrochemical aptasensor for the sensitive detection of mucin 1 ( MUC1 ) was designed based on a quadruped DNA walker driven by catalytic hairpin assembly ( CHA ). With the tetrahedral DNA nanostructure as the framework, the four legs that extend out acted as the walking chains. MUC1 bound specifically to the aptamer, activating the quadruped DNA walker and initiating the CHA reaction. The activated quadruped DNA walker hybridized with hairpin DNA1 labeled with methylene blue ( MB-HP1 ) immobilized on the electrode surface, and the toehold area of the MB-HP1 probe was exposed to bind with hairpin DNA2 labeled with ferrocene ( Fc-HP2 ), causing the quadruped DNA walker to be released. It continued to move on the electrode surface and bound with another MB-HP1. Eventually, a large number of electrochemical signal molecules were brought close to the electrode surface, while MB moved farther away. Under optimal conditions, the peak current ratio ( I Fc / I MB ) was linearly correlated with the logarithm of MUC1 concentration from 1 fg/mL to 1 ng/mL, with a detection limit as low as 0.56 fg/mL. In addition, the sensing platform demonstrated good performance in the analysis of serum samples.
研究领域:
生物分析化学
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纳米生物技术
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电化学
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生物传感器
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分子诊断学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yuanhuan Yang,Feng Gao,Ying Liang,Lichao Guo,Yang Pan,Peng Cao,Yue Zhang
The identification of Chinese medicinal herbs occupies a crucial part in the development of the food and drug market. Although molecular identification based on real-time PCR offers good versatility and uniform digital standards compared with traditional methods, such as morphology, the dependence on large-scale equipment hinders spot detection and marketable applications. In this study, we developed a DNA nanoclaw for colorimetric detection and visible on-site identification of Chinese medicines. When specific miRNA is present, the DNAzyme is activated and cleaves the substrate strand, triggering the catalytic hairpin assembly (CHA) reaction and forming branched DNA junctions on AuNP-I. This can then capture AuNP-II through hybridization and facilitate their aggregation, resulting in a noticeable color change that is observable to the naked eye. By harnessing the dual amplification of DNAzyme and CHA, this highly sensitive nanoprobe successfully achieved specific identification of Chinese medicines. This offers a new perspective for on-site testing in the herbal market.
研究领域:
生物技术
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纳米技术
·
药学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-10
·
IF 5.3
Hong Tingting,Zhou Qi,Liu Yilian,Ji Yibing,Tan Songwen,Zhou Wenhu,Cai Zhiqiang
Innovative chiral capillary silica monoliths (CSMs) were developed based on DNA nanoflowers (DNFs). Baseline separation of enantiomers such as atenolol, tyrosine, histidine, and nefopam was achieved by using DNF-modified CSMs, and the obtained resolution value was higher than 1.78. To further explore the effect of DNFs on enantioseparation, different types of chiral columns including DNA strand containing the complementary sequence of the template (DCT)-modified CSMs, DNF 2 -modified CSMs, and DNF 3 -modified CSMs were prepared as well. It was observed that DNF-modified CSMs displayed better chiral separation ability compared with DCT-based columns. The intra-day and inter-day repeatability of model analytes’ retention time and resolution kept desirable relative standard deviation values of less than 8.28%. DNF 2 /DNF 3 -modified CSMs were able to achieve baseline separation of atenolol, propranolol, 2′-deoxyadenosine, and nefopam enantiomers. Molecular docking simulations were performed to investigate enantioselectivity mechanisms of DNA sequences for enantiomers. To indicate the successful construction of DNFs and DNF-modified CSMs, various charaterization approaches including scanning electron microscopy, agarose gel electrophoresis, dynamic light scattering analysis, electroosmotic flow, and Fourier-transform infrared spectroscopy were utilized. Moreover, the enantioseparation performance of DNF-modified CSMs was characterized in terms of sample volume, applied voltage, and buffer concentration. This work paves the way to applying DNF-based capillary electrochromatography microsystems for chiral separation. Graphical abstract
研究领域:
分析化学
·
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 6
·2406-2412
·
IF 1.821
Weiwen Hu,Qiong Hu,Lianzhi Li,Jinming Kong,Xueji Zhang
In this work, an efficient method for the sequence-specific detection of DNA based on a morpholino-functionalized silicon chip platform has been proposed. Briefly, morpholino was first immobilized on the surface of a silicon chip using 3-aminopropyltriethoxysilane (APTES) as the silane coupling agent and 1,4-phenylenediisothiocyanate (PDITC) as the cross-linker and then hybridized with DNA in the ensuing step. The fluorescence label was introduced by strongly binding Rhodamine B, which contains a terminal carboxylic group, with DNA by means of phosphate–zirconium–carboxylate coordination reaction. X-ray photoelectron spectroscopy (XPS) was used to characterize the silicon surface. Under optimal conditions, the morpholino-functionalized silicon chip presented a great linear relationship between the fluorescence intensity and the logarithm of target DNA concentrations in the range from 1 pM to 1 nM with a detection limit of 4.52 pM. Furthermore, fully complementary versus single-base mismatched, three-base mismatched and non-complementary DNA could be effectively identified. The chip showed excellent stability because it could be reused for another hybridization experiment after denaturing the morpholino–complementary DNA duplex. In addition, the chip rendered satisfactory analytical performance for the detection of DNA in serum samples, thus exhibiting practical significance. Morpholino-functionalized silicon chips display good sensitivity and selectivity for the detection of DNA and promising applications in single-nucleotide polymorphisms (SNPs).
研究领域:
生物化学
·
分子生物学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）