Comparative Biochemistry and Physiology D-Genomics & Proteomics
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2026
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IF 2.9
Yufei Liu,Xiaoli Yao,Gazahegn Wakjira Yadata,Tingyao Zhu,Jianlin Guo,Yichao Yue,Lu Cao,Minbo Zhang,Jiahe Chen,Shoujie Tang,Jinliang Zhao
Artificial diet slowed early evacuation yet hastened overall gastric emptying. • The artificial diet group had markedly elevated digestive enzyme activities. • Fish fed artificial diet showed early immune responses, then metabolic shifts. Postprandial digestion and metabolism are fundamental to understanding how mandarin fish ( Siniperca chuatsi ) respond to artificial diets. However, whether artificial diets alter the temporal patterns of postprandial digestion and metabolism within a single feeding cycle remains unclear. In this study, fish fed live prey (L) or an artificial diet (A) were sampled at 0, 3, 6, 9, 12, 18, and 24 h postprandially to evaluate gastric evacuation, digestive activity, and blood biochemical parameters, while hepatic transcriptomic responses were analyzed at 0, 3, 12, and 24 h. Gastric evacuation in both groups followed a square-root model, but exhibited markedly different patterns. During the early postprandial phase, the L group showed a progressive reduction in stomach contents, whereas the A group exhibited a transient increase in stomach wet weight; thereafter, stomach contents declined in both groups, with model-estimated complete evacuation occurring earlier in the A group. The A group showed elevated digestive enzyme activity, particularly α-amylase, and a faster and sustained increase in blood glucose. During the early postprandial phase, energy metabolism pathways, including oxidative phosphorylation, glycolysis/gluconeogenesis, pyruvate metabolism, and fatty acid metabolism, were more prominent in the L group, whereas immune-related pathways were more prominent in the A group; at later stages, glucose and lipid metabolism, PPAR signaling, and protein turnover became prominent in the A group. Collectively, artificial diets reshape postprandial physiology in mandarin fish. These findings provide a dynamic physiological perspective on the adaptation of mandarin fish to formulated feed. Download: Download high-res image (170KB) Download: Download full-size image
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高影响因子
ULTRASONICS SONOCHEMISTRY
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2025
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IF 9.7
Yongjie Zhou,Zihan Guo,Sam K.C. Chang,Yan Zhang,Hui Hong,Yongkang Luo,Yuqing Tan
Silver carp viscera, a lipid‑rich byproduct typically discarded, poses environmental pollution and resource wastage. Utilizing this byproduct for fish oil extraction offers a sustainable alternative; however, conventional enzymatic methods are inefficient, labor‑intensive to optimize, and yield oil susceptible to rapid oxidation. For the first time, this study developed an integrated ultrasound pretreatment and artificial neural network-genetic algorithm (ANN‑GA) strategy to maximize fish oil extraction efficiency, followed by microencapsulation in gelatin-hexametaphosphate complex coacervation microcapsules (GSM). The ANN demonstrated excellent predictive accuracy (R 2 = 0.99). Under GA-optimized conditions (ultrasonic power: 150 W, ultrasound pretreatment: 20.75 min, protease dosage: 6889 U/g protein, hydrolysis time: 1.75 h), extraction efficiency reached 79.44 %, producing oil with 46.23 % unsaturated fatty acids. Further optimization of encapsulation parameters increased GSM encapsulation efficiency (EE) to 93.33 %, surpassing gelatin‑only microcapsules (GM, 84.22 %). FT‑IR analysis indicated that electrostatic interactions induced a more ordered protein secondary structure in GSM, with α-helix and β-sheet contents rising by 5.54 % and 6.09 %, respectively. Consequently, GSM exhibited enhanced thermal stability ( T max = 98.1 °C vs. 66.2 °C for GM), storage stability (EE unchanged for 30 days at 4 and 25°C), and oxidative stability (peroxide value approximately one-third that of GM after 10 days at 60 °C). In vitro digestion revealed GSM’s controlled release capability, liberating only 9.3 and 56 % of encapsulated fish oil during the gastric and intestinal phases, respectively. Sensory evaluation indicated significantly reduced fatty and fishy odors. Overall, this work provides a theoretical foundation for sustainable production of high‑quality freshwater fish oil.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
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IF 9.8
Hui Yu,Yier Li,Yuzhen Zhu,Samad Tavakoli,Xingyan Li,Qianqian Ouyang,Luming Deng,Saiyi Zhong,Chunyu Xiao,Yumeng Wang,Hui Hong,Kefeng Wu,Hui Luo
Fish maw (FM), the dried swim bladders of fish, is highly valued for its nutritional and pharmacological properties due to its high collagen content, a source of fish gelatin. In traditional Chinese medicine, FM is typically processed with clamshell powder (CP), called CP-FM. This study evaluated the effects of stir-frying FM with CP on its physicochemical properties. These results revealed that CP-FM significantly enhanced physicochemical properties compared to FM. Fourier-transform infrared spectroscopy indicated strong interactions between collagen and calcium ions (Ca 2+ ), and electron microscopy confirmed the presence of peptide‑calcium chelates in CP-FM. Additionally, integrative omics analysis uncovered alterations in the abundance of collagen and modifications in N-glycosylation sites, particularly in type I collagen. These modifications enhanced the Ca binding capability of the collagen, thereby improving the stability and overall quality of FM gelatin. This research highlights the positive impact of CP as an ingredient during the stir-frying process and provides valuable insights into the implications of processing dried seafood.
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PubMed
高影响因子
Food Bioscience
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2025
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IF 5.9
Wenyuchu Chen,Fang Tian,Yongjing Xu,Yujin Li,Weiliang Guan,Chenyang Lu,Sheng Hu,Luyun Cai
Fish oils (FOs) are rich in omega-3 polyunsaturated fatty acids, which provide several health benefits. However, their oral bioavailability and stability are often limited due to the oxidation of polyunsaturated fatty acids, which degrade the oil's nutritional and sensory properties over time. In this study, FO microcapsules were prepared using pea protein isolate (PPI), β-cyclodextrin (β-CD), and carbon quantum dots (CQDs) as wall materials through a freeze-drying method. The results demonstrated that the use of PPI/β-CD/CQDs composite significantly improved the encapsulation efficiency, stability, and antioxidant properties of the microcapsules, suggesting a promising approach for enhancing the shelf-life and health benefits of fish oil. Additionally, the peroxide value (POV) and thiobarbituric acid (TBA) values of the microcapsules increased with temperature and storage time. Notably, the incorporation of CQDs markedly improved the antioxidant properties of the microcapsules, thereby extending their storage stability. In vitro gastrointestinal digestion simulations demonstrated that microencapsulation facilitated the absorption of beneficial substances in fish oil within the human gut. This study offers valuable insights into the development of more efficient fish oil microcapsules for potential applications in food and pharmaceutical applications.
研究领域:
食品科学
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营养学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Wen-Long Cheng,Zi-Qiang Cheng,Zi-Zi Hu,Ming-Ming Hu,Guang-Xian Liu,Lin-Feng Yuan,Yan-Hui Fu,Xiao-Mei Sha,Zong-Cai Tu
Aqueous zinc-ion batteries (ZIBs) have emerged as promising next-generation energy storage solutions due to their high safety. Hydrogel electrolytes have garnered intensive interest for their applications in flexible ZIBs, combining mechanical flexibility with dendrite-suppression capabilities. However, conventional hydrogels suffer from three critical limitations: insufficient mechanical robustness, interfacial instability, and excessive salt dependence. Herein, we report a novel fish gelatin (FG)-modified polyacrylamide (PAM) hydrogel electrolyte fabricated via a facile protein chain network repair strategy. The optimized FG/PAM hydrogel electrolyte with 1 M ZnSO 4 exhibits superior mechanical properties, enabling ZIBs with flexible dendrite-free and prolonged lifespan. Specifically, the FG-modified hydrogel achieved a compressive strength exceeding 1078 kPa under 90 % compression and showed strong adhesion to diverse substrates. Electrochemically, the FG/PAM electrolyte exhibits excellent corrosion resistance and zinc-nucleation regulation capabilities, delivering a stable coulombic efficiency (CE) of 99.26 % and cycling stability over 2238 h. These results highlight the FG/PAM hydrogel in addressing mechanical integrity, interfacial compatibility, and long-term cyclability, providing a viable pathway for advanced flexible energy storage devices.
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高影响因子
FOOD AND BIOPRODUCTS PROCESSING
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2025
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IF 3.4
Peilan Xie,Zhenqian Tang,Ba Guan,Yimeng Tang,Qiyou Chen,Jiaying Ou,Kangmin Lin,Zhuo Jiang
Fish oil faces challenges like oxidation, low bioavailability, and poor sensory properties. While microencapsulation improves stability, traditional methods suffer from poor monodispersity, core damage, and limited wall material control. In this study, the plug-and-play microfluidic system overcame these limitations by precisely controlling emulsion formation and bypassing charge-matching requirements. Based on microfluidic technology, a plug-and-play microfluidic chip (single-channel and three-parallel channel) was used to control preparation (coefficient of variation < 5 %, shell thickness from 163 to 592 μm, core number from 1 to 5) and scaled-up production of fish oil microcapsules. A multi-dimensional wall material screening platform was developed via ionotropic crosslinking, using sodium alginate as the network skeleton and integrating gum arabic (GA) for emulsifying stability and low-methoxyl pectin (LM) for pH responsiveness. The results showed that the ternary composite wall material system (TCWMS) exhibited lower peroxide value (POV) and Malondialdehyde (MDA) values, and strong hydrogen bonding interactions. The multi-dimensional wall material screening platform and plug-and-play microfluidic chip were built to enable stable, controllable industrial preparation of monodisperse oily microcapsules.
研究领域:
食品科学
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微胶囊技术
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药物递送
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生物材料
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功能食品
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控释系统
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高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2021
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IF 4.581
Abeer M. Hegazy,Nan Chen,Hanzuo Lin,Sarath Babu V.,Feng Li,Youcheng Yang,Zhendong Qin,Fei Shi,Jun Li,Li Lin
An increasing important area in immunology is the process cell death mechanism, enabling the immune system triggered thru extrinsic or intrinsic signals to effectively remove unwanted or virus infected cells called apoptosis. A recently isolated infectious Snakehead fish vesiculovirus (SHVV), comprising negative strand RNA and encoded viral matrix (M) proteins, is responsible for causing cytopathic effects in infected fish cells. However, the mechanism by which viral M protein mediates apoptosis has not been elucidated. Therefore, in the present experiments, it was investigated the regulatory potential of apoptosis signals during SHVV infection. By employing the model of SHVV infection in SSN-1 cells, the accelerated apoptosis pathway involves an intrinsic pathway requiring the activation of caspase-9 but not caspase-3 or -8. In the groups of infection (SHVV) or treatment (hydrogen peroxide) were induced apoptotic morphological changes and indicated the activation of the main caspases, i.e.; executioner caspase-3, initiators caspase-8 and caspase-9 using colorimetric assays. Turning to the role of viral M protein when it was overexpressed in SSN-1 cells, it was indicated that the viral M gene alone has the ability to induce apoptosis. To elucidate the mechanism of apoptosis in SSN-1 cells, the activation inhibitors of main caspases were used showing that inhibiting of caspase-3 or caspase-8 activation did not seize induction of apoptosis in virus-infected SSN-1 cells. However, the inhibiting of caspase-9 activation reduced significantly the apoptosis initiation process and sharply the expression of viral M gene, suggesting that SHVV plays a major role in the early induction of apoptosis by caspase-9. Interestingly, there were also differences in the mitochondrial membrane potential after the apoptotic induction of caspases, which confirm that caspase-9 is primarily responsible for the cleavage of caspases during apoptosis. Taken together, these findings can therefore be assumed that viral M protein induces apoptosis via the intrinsic apoptotic pathway in SHVV infecting SSN-1 cells.
研究领域:
免疫学
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病毒学
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细胞生物学
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DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
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2019
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IF 2.17
Bing-Jie Jia,Jun Huang,Ju-Xiang Liu,Jian-Ping Wang
In this study, 4-nitrotoluene (NT) was used as dummy template to synthesize a molecularly imprinted polymer that was highly specific for chloramphenicol. The polymer was coated in the wells of 96-well microplates as recognition reagent to develop a chemiluminescence method. The analyte solution and an enzyme-labelled hapten were added into the wells to perform competition, and the light signal was induced with a highly efficient luminol-H2O2-4-(imidazol-1-yl)phenol system. Then, the optimized method was used to determine chloramphenicol in meat (chicken, pork and fish), and the limit of detection (LOD) was 5.0 pg g−1. Furthermore, the polymer-coated plate could be reused four times, and one test could be finished within 20 min. The recoveries from the standard fortified blank meat samples were in the range of 71.5–94.4%. Therefore, this method could be used as a useful tool for routine screening the residue of chloramphenicol in meat samples.
研究领域:
分析化学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
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2026
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IF 6.6
Huibo Meng,Yi Ji,Wenchun Jiang,Yi Zhou,Yanfang Yu
Static mixers (SM) are widely used for reaction and heat transfer intensification, yet further improvements in mixing efficiency remain highly desirable for fast reactive systems. Accordingly, bionic fishtail-shaped static mixers (FTSM) were proposed and systematically compared with a conventional Kenics static mixer (KSM) for Mg(OH) 2 precipitation using experiments and CFD simulations. The results indicated that FTSM-V generated continuously distributed longitudinal and transverse vortices, which enhanced turbulence intensity and promoted a more continuous decrease in COV along the axial direction. The coefficient of variation (COV) of the FTSM-V decreased to 0.010, which is approximately 30% lower than that of KSM, at z / l = 3.5 and Re = 6000. In KSM, the stepwise rise in product concentration occurs at the junction between the 1# and 2# elements, while it occurs mainly in the upstream region of the 4# element in FTSM-V. The yield using FTSM-V was 13.7% higher than that of KSM. A maximum volumetric specific power dissipation of 520 kWh/m 3 was achieved, representing a 36.2% improvement over non-optimal conditions, at C total = 5.25 mol/L and R = 3. This work provided a novel fishtail-shaped static mixer design, enabling an efficient and scalable strategy for intensifying mixing–reaction coupling in fast reactive systems.
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高影响因子
Desalination and Water Treatment
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2024
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IF 1
Xiaoyu Zhang,Jian Lu,Guodong Zhang,Dejie Cui
Fish feces and residues are a kind of common waste in aquaculture system to require timely handling while aquaculture wastewater generally contains antibiotics to require efficient treatment. This study prepared biochar by using fish feces and residues to investigate the removal of typical fluoroquinolone enrofloxacin by biochar. Biochar showed the sheet-dot mixed structure with multiple elements on surface. The content of carbon/nitrogen/hydrogen of biochar made of fish feces and residues reached 46.5%/3.1%/2.5%. The removal efficiency of enrofloxacin reached 94.7% with biochar dosage of 1 g/L. The adsorption capacity of biochar at pH of 2 or 10 was higher than that at the other pH values. Adsorption capacity of biochar increased by 10.6% from 15°C to 25°C to reach 9.47 mg/g at 25°C while that slightly increased from 25°C to 35°C. Pseudo-first-order model was the best model with R 2 = 0.9982 for describing adsorption kinetics of enrofloxacin by biochar. Langmuir model could better fit results of isotherm for enrofloxacin by biochar with Langmuir constant of 0.38–1.65 L/mg. Biochar used 3–4 times still kept good removal efficiency for enrofloxacin. The prepared biochar exhibited good application potential. The findings of this study provide new idea for treating aquaculture solid wastes and wastewater.
研究领域:
环境科学
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水处理
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废弃物管理
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吸附技术
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2026
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IF 4.4
Tian Yu Qing,Wu Meng Yao,Lu Jiao Yang,Huang Wei Tao
This study explores the untapped potential of fish scales (FS) as bioinspired platforms for antibacterial applications through a dual approach: (1) systematic characterization of natural scale architectures and (2) development of functional silver nanocomposites (FS-AgNCs). Comprehensive analysis revealed zonal differentiation in grass carp scales, with outer surfaces exhibiting ridge patterns (19–54 μm width) and protrusions, while inner surfaces remained smooth. The covered area (25.78% of surface) showed higher organic content (37.5%) than the exposed zone (30% organic). Leveraging these natural features as nucleation sites, we developed FS-AgNCs via in situ reduction, forming uniformly distributed Ag nanoparticles (248 ± 3 nm and 575 ± 9 nm populations). The nanocomposites demonstrated potent antibacterial activity, achieving 10.4 ± 0.2 mm inhibition zones and 97% killing efficiency against pathogenic bacteria—significantly outperforming bare scales ( P < 0.05). In vivo testing confirmed both biosafety and delayed mortality in Aeromonas hydrophila -challenged fish, with 100% survival maintained over 4 days in treated groups compared to 62.5% mortality within 24 h in controls. This work establishes a sustainable paradigm for developing biocompatible antimicrobial materials by integrating natural biomimetic structures with nanotechnology, offering promising applications in aquaculture and medical coatings.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2026
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IF 6.1
Xue Wang,Zhaoyang Zhou,Shuang Jiang,Qi Sun,Xianlu Lei,Yong Xie,Tao Le
Norfloxacin (NOR) has been widely applied in animal husbandry and aquaculture because of its potent antibacterial activity, broad-spectrum efficacy and cost-efficient. Nevertheless, its inappropriate use may result in teratogenic and carcinogenic risks to humans, making the accurate and rapid detection of NOR residues in food critically important. In this study, a strong affinity (Kd = 84 nM) and high selectivity anti-NOR aptamer was obtained by screening with Graphene Oxide Systematic Evolution of Ligands by Exponential Enrichment (GO-SELEX), and a colorimetric aptasensor was fabricated. The limit of detection of the aptasensor was 1.04 ng/mL, and the linear range was 1.56-100 ng/mL. Average recoveries of the aptasensor in shrimp and fish sample analyses ranged from 89.33% to 103.24%, accompanied by coefficients of variation from 3.63% to 9.43%, indicating good analytical precision and accuracy. The aptasensor constructed in this study showed high consistency with the High-Performance Liquid Chromatography (HPLC) for NOR detection, indicating that it is an efficient and promising sustainable strategy for monitoring NOR.
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PubMed
高影响因子
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