COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Qiwei Zhong,Wenhua Li,Xiuping Su,Geng Li,Ying Zhou,Subhas C. Kundu,Juming Yao,Yurong Cai
Despite superior clinical handling, excellent biocompatibility, biodegradation property of calcium phosphate needs to be improved to coincide with the rate of new bone formation. In this study, spherical CaCO 3 are fabricated in the presence of the silk sericin and then transformed into porous hydroxyapatite (HAP) microspheres via hydrothermal method. The degradation behavior of obtained CaCO 3 , HAP and their mixture is first investigated in vitro . The result demonstrates that the weight loss of HAP microspheres are almost 24.3% after immersing in pH 7.40 Tris-HCl buffer solution for 12 weeks, which is far slower than that of spherical CaCO 3 (97.5%). The degradation speed of the mixtures depends on the proportion of CaCO 3 and HAP. The mixture with higher content of CaCO 3 possesses a quicker degradation speed. The obtained CaCO 3 and HAP microspheres are injected into subcutaneous tissue of ICR mice with the assistance of sodium alginate. The result in vivo also shows an obvious difference of degradation speed between the obtained CaCO 3 and HAP microspheres, implying it is feasible to modulate the degradation property of the mixture through changing the proportion of CaCO 3 and HAP The good cytocompatibility of the two kinds of microspheres is proved and a mild inflammation response is observed only at early stage of implantation. The job offers a simple method to modify the degradation properties of biomaterial for potential use in bone tissue engineering.
研究领域:
材料科学
·
生物材料
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
Environmental Science-Advances
·
2023
·Vol. 2
·期 6
·837-847
·
IF 0
Hussain Ijaz,Jin-Xin Zi,Si-Si Liu,Qi-Si Cai,Sheng-Ming Cheng,Zong-Xi Zhao,Guang-Guo Ying,Andy J. Sweetman,Chang-Er Chen
The extensive use of artificial sweeteners (ASs) has raised concerns regarding their presence, fate, and detrimental effects in water resources. It is thus of great interest for researchers and regulators to acquire a sensitive, robust, and practical sampling technique for monitoring ASs. In the present study, an effective and established passive sampling method based on the diffusive gradients in thin films (DGT) technique was proposed to measure ASs in water. A mixed binding gel of WAX-XDA1 (3 : 1, w/w) was used for the first time in DGT. The selected mixed binding gel exhibited strong and rapid adsorption of ASs and its total binding capacity was over 20.6 μg per disc. 1% (v/v) ammonium hydroxide-methanol was demonstrated to be an excellent elution solvent for ASs with the elution efficiencies being 80.2–104.9%. The linear accumulation of ASs by WAX-XDA1-DGT matched well with the theoretical values, indicating that the current configuration performs in accordance with the DGT principle. The assembled WAX-XDA1-DGT sampler possessed a good tolerance to a wide range of pH (4–8), ion strength (0–0.5 M), and dissolved organic matter (0–10 mg L−1). In wastewater, comparable or lower DGT-measured concentrations for the ASs were observed compared to the spot sampling results. To the best of our knowledge, no studies have yet been conducted to in situ monitor ASs by using the DGT-based method. This study demonstrates the robustness and reliability of WAX-XDA1-DGT for measuring ASs under a wide range of freshwater circumstances in aquatic environments.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
Frontiers in Pharmacology
·
2021
·
IF 5.811
Wang Jinhui,Chen Feifei,Jiang Hui,Xu Jia,Meng Deru,Geng Peiwu,Dai Dapeng,Hu Jingbo,Zhou Yunfang,Zhou Quan,Wang Shuanghu
Poziotinib is an orally active, irreversible, pan-HER tyrosine kinase inhibitor used to treat non-small cell lung cancer, breast cancer, and gastric cancer. Poziotinib is currently under clinical investigation, and understanding its drug-drug interactions is extremely important for its future development and clinical application. The cocktail method is most suitable for evaluating the activity of cytochrome P450 enzymes (CYPs). As poziotinib is partially metabolized by CYPs, cocktail probes are used to study the interaction between drugs metabolized by each CYP subtype. Midazolam, bupropion, dextromethorphan, tolbutamide, chlorzoxazone, phenacetin, and their metabolites were used to examine the effects of poziotinib on the activity of cyp1a2, 2b1, 2d1, 2c11, 2e1, and 3a1/2, respectively. The in vitro experiment was carried out by using rat liver microsomes (RLMs), whereas the in vivo experiment involved the comparison of the pharmacokinetic parameters of the probes after co-administration with poziotinib to rats to those of control rats treated with only probes. UPLC-MS/MS was used to detect the probes and their metabolites in rat plasma and rat liver microsomes. The in vitro results revealed that the half-maximal inhibitory concentration values of bupropion and tolbutamide in RLMs were 8.79 and 20.17 μM, respectively, indicating that poziotinib showed varying degrees of inhibition toward cyp2b1 and cyp2c11. Poziotinib was a competitive inhibitor of cyp2b1 and cyp2c11, with Ki values of 16.18 and 17.66 μM, respectively. No time- or concentration-dependence of inhibition by poziotinib was observed toward cyp2b1 and cyp2c11 in RLMs. Additionally, no obvious inhibitory effects were observed on the activity of cyp1a2, cyp2d1, cyp2e1, and cyp3a1/2. In vivo analysis revealed that bupropion, tolbutamide, phenacetin, and chlorzoxazone showed significantly different pharmacokinetic parameters after administration (p < 0.05); there was no significant difference in the pharmacokinetic parameters of dextromethorphan and midazolam. These results show that poziotinib inhibited cyp2b1 and cyp2c11, but induced cyp1a2 and cyp2e1 in rats. Thus, poziotinib inhibited cyp2b1 and cyp2c11 activity in rats, suggesting the possibility of interactions between poziotinib and these CYP substrates and the need for caution when combining them in clinical settings.
研究领域:
药理学
·
临床医学
·
生物化学
✓ 已验证
DOI
高影响因子
Research
·
2022
·
IF 11.036
Yiqiu Fei,Shuobo Zhang,Shengyi Han,Bo Qiu,Yanmeng Lu,Weixing Huang,Fang Li,Deying Chen,Björn Berglund,Hang Xiao,Lanjuan Li,Mingfei Yao
Currently approved therapeutical strategies for inflammatory bowel diseases (IBD) suffer from variable efficacy and association with risk of serious side effects. Therefore, efforts have been made in searching for alternative therapeutics strategies utilizing gut microbiota manipulation. In this study, we show that the probiotic strain Ligilactobacillus salivarius Li01 (Li01) and the phytochemical prebiotic resveratrol (RSV) have synergistic effect in ameliorating colitis in mice. Oral coadministration of Li01 (10 9 CFU/d) and RSV (1.5 g/kg/d) promoted restoration of various inflammatory injuries and gut microbiota composition, exhibiting a favorable anti-inflammatory effect in DSS-induced colitis mice. The combination treatment was associated with reductions in the levels of proinflammatory cytokines IL-1 β and IL-6 and increases in the levels of the anti-inflammatory cytokine IL-17A in mouse serum. Moreover, the combination treatment was found to alter the composition and metabolism of the gut microbiota, especially influencing the production of short chain fatty acids and anti-inflammatory related molecules. The mechanism underlying the improved anti-inflammatory effect from the RSV and Li01 combination treatment was found to be associated with the environmental sensor mammalian aryl hydrocarbon receptor (AHR) and tryptophan metabolism pathway. Administration of RSV in combination with Li01 in different mouse model led to enhanced conversion of RSV into metabolites, including dihydroresveratrol (DHR), resveratrol-sulfate, and resveratrol-glucuronide. DHR was found to be the dominant metabolite of RSV in conventional and colitis mice. An increased DHR/RSV ratio was confirmed to activate AHR and contribute to an enhanced anti-inflammatory effect. DHR is considered as a potential AHR ligand. The DHR/RSV ratio also affected the serotonin pathway by controlling the expression of Tph1, SERT, and 5-HT 7 R leading to amelioration of colitis in mice. Our data suggest that treatment with a combination of Li01 and RSV has potential as a therapeutic strategy for IBD; further investigation of this combination in clinical settings is warranted.
研究领域:
药理学
·
免疫学
·
微生物学
·
胃肠病学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Ziyu Wang,Wenkang Zhang,Qilin Gu,Zheng Liang,Zhaoxiang Zhong,Weihong Xing
Ceramic membranes with tailored surface wettability have gained increasing attention for improving their separation efficiency and antifouling ability, while most of the previous work focused on the underwater oleophobic behavior. In this work, in-air highly oleophobic and hydrophilic SiC membranes were achieved through the UV irradiation triggered polymerization and controlled crosslinking between oleophobic perfluorodecyl acrylate (TFOA) and hydrophilic methacrylic acid (MAA). These key processing parameters including MAA content, soaking time and irradiation duration were systematically studied through examining the changes in porous microstructure, surface wettability and separation performance in oil-in-water emulsion. Results showed that the flux attenuation rate of SiC membranes can be effectively reduced by regulating the content of hydrophilic components. Due to the alternation of the surface wettability to highly oleophobic in air, the oil droplets can hardly attach on the surface of modified SiC membranes at the beginning of filtration process, and the membrane fouling was significantly slow down yet dominated by the intermediate blocking. Correspondingly, a combined cleaning procedure was proposed to regenerate the fouled SiC membranes. Further, the antifouling ability of modified and original SiC membranes was comparatively studied under the conditions of a fixed permeate volume (15 ml) and various oil concentrations (200 ∼ 1000 ppm) in the separation of oil-in-water emulsion. The advantages of modified SiC membranes become more notable when used for highly concentrated oil–water emulsion as reflected by the slower flux decline and higher stable flux. This work provides a feasible pathway to realize the in-air highly oleophobic and hydrophilic surface on ceramic membranes, and primarily demonstrates the advantages of antifouling ability in oil-in-water emulsion separation.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Hongjian Kang,Jixia Wang,Yanfang Liu,Feifei Huang,Han Zhou,Xiaomin Xie,Qing Xu,Xinmiao Liang,Xingya Xue
Traditional Chinese medicines (TCMs) are popular in clinic because of their safety and efficacy. They contain abundant natural active compounds, which are important sources of new drug discovery. However, how to efficiently identify active compounds from complex ingredients remains a challenge. In this study, a method combining UHPLC-MS/MS characterization and in silico screening was developed to discover compounds with dopamine D2 receptor (D2R) activity in Stephania epigaea ( S. epigaea ). By combining the compounds identified in S. epigaea by UHPLC-MS/MS with reported compounds, a virtual library of 80 compounds was constructed for in silico screening. Potentially active compounds were chosen based on screening scores and subsequently tested for in vitro activity on a transfected cell line CHO-K1-D2 model using label-free cellular phenotypic assay. Three D2R agonists and five D2R antagonists were identified. (-)-Asimilobine, N -nornuciferine and (-)-roemerine were reported for the first time as D2R agonists, with EC 50 values of 0.35 ± 0.04 μM, 1.37 ± 0.10 μM and 0.82 ± 0.22 μM, respectively. Their target specificity was validated by desensitization and antagonism assay. (-)-Isocorypalmine, (-)-tetrahydropalmatine, (-)-discretine, (+)-corydaline and (-)-roemeroline showed strong antagonistic activity on D2R with IC 50 values of 92 ± 9.9 nM, 1.73 ± 0.13 μM, 0.34 ± 0.02 μM, 2.09 ± 0.22 μM and 0.85 ± 0.08 μM, respectively. Their kinetic binding profiles were characterized using co-stimulation assay and they were both D2R competitive antagonists. We docked these ligands with human D2R crystal structure and analyzed the structure-activity relationship of aporphine-type D2R agonists and protoberberine-type D2R antagonists. These results would help to elucidate the mechanism of action of S. epigaea for its analgesic and sedative efficacy and benefit for D2R drug design. This study demonstrated the potential of integrating UHPLC-MS/MS with in silico and in vitro screening for accelerating the discovery of active compounds from TCMs.
研究领域:
药理学
·
生物信息学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CNS Neuroscience & Therapeutics
·
2024
·Vol. 30
·期 10
·e70076
·
IF 4.8
Jing An,Lulu Wen,Haiyang Yu,Zhongqi Bu,Juan Feng
Aims Insulin-like growth factor binding protein 2 (IGFBP2) is implicated in various neurodegenerative diseases. However, its role in Parkinson's disease (PD) is unclear. Methods PD rat model was established by 6-OHDA injection. After 3 weeks, mRNA-seq was conducted. Rats received rIGFBP2 via intra-MFB injection 6 h prior to 6-OHDA infusion, and the effect of IGFBP2 in PD rats was investigated by western blotting, IHC, specific kits, JC-1 staining, and TUNEL analysis. In vitro, PC12 cells were treated with 6-OHDA, and CCK-8, specific kits, Hoechst 33258 staining, Western blotting, and JC-1 staining were performed to assess the IGFBP2's role. Results mRNA-seq revealed DEGs in PD, with attention to downregulated IGFBP2. rIGFBP2 treatment aggravated neurobehavioral deficits, decreased TH expression, Ψm, ATP level and SOD, GSH-Px activities but increased α-synuclein, ROS, MDA, mitochondrial cytochrome c contents, cell apoptosis in 6-OHDA-lesioned rats, which might be mediated through inactivating IGF-1R/AKT pathway. In 6-OHDA-treated PC12 cells, rIGFBP2 aggravated cell injury, demonstrated by decreased cell viability and increased apoptosis, oxidative stress, and mitochondrial dysfunction. Co-treatment with rIGFBP2 and rIGF-1 partially reversed the effect of rIGFBP2 on cell damage. Conclusion IGFBP2 exacerbates neurodegeneration in PD through increasing oxidative stress, mitochondrial dysfunction, and apoptosis via inhibiting IGF-1R/AKT pathway.
研究领域:
神经科学
·
分子生物学
·
药理学
·
病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Tianbin Bai,Yanhua Liu,Zhiying Nan,Zhuanli Zhang,Jianbin Zhang,Dianming Li,Libang Feng
The separation of oil/water pollution is of great importance to environmental protection. However, the development of superwetting materials for efficient separation of surfactant-stabilized oil/water emulsions still faces challenges. As agricultural waste, sugarcane straw contains many hydrophilic groups and multilayered pore structures, making it suitable for purifying oil/water emulsions by simple chemical modification treatments. Herein, sugarcane-based superhydrophobic membranes (SSMs) were prepared by a facile vacuum impregnation method in hexadecyltrimethoxysilane (HDTMS) solution. The unique bulge-like microstructures were constructed in the intrinsic pores of sugarcane, which significantly increased the roughness and resulted in superhydrophobicity with the water contact angle of 153.5°. More importantly, the bulge-like structure is easy to break the emulsion and thus SSMs offer excellent separation efficiency (> 99 %), suitable fluxes (368 L•m −2 •h −1 ) and strong cycling stability for surfactant-stabilized water-in-oil emulsions. In addition, SSMs can separate oil/water mixtures and directly adsorb oil from water with adsorption rates exceeding 99 %. SSMs have good durability and anti-mold adherence properties, and they do not lose their unique wetting properties even in harsh environments. More importantly, SSMs can be degraded in soil at the end of their life cycle, avoiding secondary pollution for the environment. This simple, in-situ impregnation technique offers a novel way to create green, inexpensive and stable biomass membranes for effective water-in-oil emulsion separation, showing great promise for use in oily wastewater treatment.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2025
·
IF 8.3
Zihao Xie,Qian Yang,Xiaomin Xu,Deqing He,Yongning Yi,Hao Qiu,Huangang Shi,Yingping Pang,Wei Wang,Chao Su
The main obstacle in the commercial application of aqueous zinc-air batteries (ZABs) are emerging viable, sustainable, and safe alternatives in electrochemical energy conversion technologies. However, the practical realization of ZABs is impeded by four-electron oxygen evolution (OER) and reduction reactions (ORR) and their sluggish kinetics. Herein, we propose a niobium and fluorine co-doping strategy to regulate in situ Ba and Sr exsolution, inducing the formation of amorphous/crystalline Ba 0.6 Sr 0.4 Co 0.8 Fe 0.2 O 3-δ heterointerfaces with an alkali treatment. Associated with the formation of a low valence state of cobalt, an abundant oxygen defects, and amorphous/crystalline feature, the catalyst enables enhanced oxygen binding energy in the OER and ORR activities. The optimized bifunctional perovskite oxide (BSCFeN-F al ) catalysts present an excellent oxygen electrocatalytic activity and stability, with much lower OER and ORR overpotentials than other perovskite oxides in this work and with negligible performance decay in accelerated durability testing. When used as an air-electrode, the BSCFeN-F al demonstrates excellent performance, achieving high power density and remarkable cycling stability. This work highlights the essential function of the heterostructure interface in oxygen electrocatalysis, opening a new avenue to advanced neutral metal-air batteries.
研究领域:
材料科学
·
电化学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yuanyuan Zhong,Yangang Chen,Miaoya Zhang,Hao Zhang,Xiaomin Liao,Huan Jin,Jiaxin Feng,Xianan Qin
Nanoparticle size dispersion within polymer nanocomposites is crucial for ensuring material properties and performance. Monitoring the evolution of particle size distribution during processing proves to be critical for elucidating fundamental mechanisms and optimizing manufacturing parameters. The size dispersion evaluation relies on microscopy imaging of the nanoparticles inside the polymer matrix. However, current imaging techniques face significant challenges due to resolution limitations. In this study, we introduce a method that, despite having a microscopy resolution larger than the minimal particle size, effectively assesses the evolution of nanoparticle size dispersion during the fabrication process of polymer nanocomposites. We show that this method has an amplifying effect on the observation of nanoparticles with larger size, namely, the probabilities of the “residues” of the size statistics are larger than the corresponding original probabilities. We demonstrate the utility of this method to assess the agglomeration of nanoparticles during the fabrication processes of polymer nanocomposites. We prepare zinc oxide (ZnO) nanoparticles, incorporate them into polyethylene terephthalate (PET) chips, subsequently process them into ZnO/PET composite fibers, and apply the method to inspect the whole process of the fabrication. Our findings indicate that the developed method provides a reliable evaluation of nanoparticle size dispersion across different material forms. We observed that the fabrication process from ZnO/PET chips to ZnO/PET fibers increases the degree of aggregation, whereas the step from ZnO nanoparticles to ZnO/PET chips maintains a relatively fine size dispersion. Our developed method shows a novel “residue imaging” strategy and can be listed as a useful way to inspect the filler particle dispersion in polymer nanocomposites.
研究领域:
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Lu Fan,Yulong Jin,Aiguo Zhang,Youzhen Feng,Kai Wang
In this study, A fluorescent/photoacoustic dual-modal probe HD-MAO for hemicyanine derivatives was successfully designed and synthesized, achieving high-sensitivity and high-specificity detection of monoamine oxidase A (MAO-A). Through the ingenious combination of the specific MAO-A recognition group and the hemipanocyanine fluorophore, the probe produced a significant enhancement of the intramolecular charge transfer (ICT) effect after enzymatic digestion, showing the synergistic activation characteristics of fluorescence (∼730 nm) and photoacoustic (∼700 nm) signals, with a detection limit of 0.806 mg/L. In the diet-induced obese mouse model, HD-MAO successfully revealed a significant increase in liver MAO-A activity, which was highly consistent with the serum test results, providing molecular imaging evidence for the early diagnosis of metabolic diseases. The probe behaves stably under physiological conditions and shows no cross-reaction to common interferents. Serum detection experiments further confirm its clinical applicability. This study not only provides an innovative tool for the research on MAO-A-related mechanisms, but also opens up a new approach for the early diagnosis and treatment monitoring of metabolic diseases.
研究领域:
生物成像
·
分子诊断
·
酶检测
·
代谢疾病
·
生物医学光学
·
探针开发
✓ 已验证
DOI
PubMed
高影响因子
NATURE MATERIALS
·
2024
·Vol. 23
·期 12
·1686-1694
·
IF 37.2
Shi Jie,Koketsu Toshinari,Zhu Zhenglu,Yang Menghao,Sui Lijun,Liu Jie,Tang Mingxue,Deng Zhe,Liao Mengyi,Xiang Jingwei,Shen Yue,Qie Long,Huang Yunhui,Strasser Peter,Ma Jiwei
‘Anode-free’ Li metal batteries offer the highest possible energy density but face low Li coulombic efficiency when operated in carbonate electrolytes. Here we report a performance improvement of anode-free Li metal batteries using p -block tin octoate additive in the carbonate electrolyte. We show that the preferential adsorption of the octoate moiety on the Cu substrate induces the construction of a carbonate-less protective layer, which inhibits the side reactions and contributes to the uniform Li plating. In the mean time, the reduction of Sn 2+ at the initial charging process builds a stable lithophilic layer of Cu 6 Sn 5 alloy and Sn, improving the affinity between the Li and the Cu substrate. Notably, anode-free Li metal pouch cells with tin octoate additive demonstrate good cycling stability with a high coulombic efficiency of ~99.1%. Furthermore, this in situ p -block layer plating strategy is also demonstrated with other types of p -block metal octoate, as well as a Na metal battery system, demonstrating the high level of universality.
研究领域:
电化学
·
电池技术
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）