AQUACULTURE NUTRITION
·
2025
·Vol. 2025
·期 1
·5623889
·
IF 3.9
Xianglin Cao,Rongjie Yuan,Yi Guo,Mengtao Jia,Yinyin Wei,Jiameng zhou,Han Cui,Baohua Li,Jianjun Chen
L-Carnitine is widely recognized for its involvement in lipid metabolism, but its effects on muscle quality and gut health in carp have not been well studied. The research aimed to investigate how L-carnitine influences muscle quality and intestinal health in high-fat-fed carp. The study was separated into four groups that received either the standard diet, a high-fat diet (HFD), or a HFD supplemented with 500 mg/kg L-carnitine (LLC), or a HFD supplemented with 1000 mg/kg L-carnitine (HLC) for 56 days. L-Carnitine was found to significantly reduce blood lipid levels. In addition, L-carnitine increased the crude protein content and decreased the crude fat content of high-fat-fed carp muscle while improving muscle fiber morphology and muscle quality. L-Carnitine increased the expression of genes related to intestinal tight junction proteins ( claudin-2 , occludin , and zo-1 ), improved the expression of genes related to intestinal inflammation, and enhanced the physical barrier function and organization of the intestine. Analysis of intestinal flora and intestinal metabolites showed that L-carnitine increased the diversity of the intestinal flora, increased the abundance of Cetobacterium , and influenced intestinal levels of bile acids, arachidonic acid, and tryptophan-related metabolites. In conclusion, supplementation with 1000 mg/kg L-carnitine improved muscle quality and intestinal health significantly in high-fat-fed carp by regulating muscle nutrient metabolism and intestinal flora.
研究领域:
水产养殖
·
营养学
·
生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Yan Zhang,Hai-Yan Wang,Xi-Wen He,Wen-You Li,Yu-Kui Zhang
In this work, we innovatively synthesized homochiral fluorescence nano molecularly imprinted polymers (D-MIP) with dual affinity (metal ion affinity and homochiral affinity) for the specific separation and detection of L-penicillamine (L-PA), which is a core-shell structure with a SiO 2 -covered CDs core and an imprinted layer with L-PA cavities. A switch for fluorescence response was built by chelating grafted Cu 2+ , what's more, the imprinted L-PA was pre immobilized by Cu 2+ to form the directional imprinting with predetermined spatial structure. More importantly, the homochiral affinity of D-galactose in homochiral molecularly imprinted polymers (D-MIP) greatly enhanced the selective adsorption of L-PA, and D-MIP showed a high selectivity factor (α) of 3.45, which is 1.9 times that of the non-homochiral molecularly imprinted polymers (MIP). Meanwhile, D-MIP exhibited a high enantiomeric excess (ee) value of 56% for separation of racemic PA. Additionally, a high sensitive and selective method was established for the detection of L-PA.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
生物技术
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Qi-Wei Fu,Yun-Peng Zi,Wei Xu,Rong Zhou,Zhu-Yun Cai,Wei-Jie Zheng,Feng Chen,Qi-Rong Qian
Calcium phosphate-based biomaterials have been well studied in biomedical fields due to their outstanding chemical and biological properties which are similar to the inorganic constituents in bone tissue. In this study, amorphous calcium phosphate (ACP) nanoparticles were prepared by a precipitation method, and used for preparation of ACP-poly(d,l-lactic acid) (ACP-PLA) nanofibers and water-soluble drug-containing ACP-PLA nanofibers by electrospinning. Promoting the encapsulation efficiency of water-soluble drugs in electrospun hydrophobic polymer nanofibers is a common problem due to the incompatibility between the water-soluble drug molecules and hydrophobic polymers solution. Herein, we used a native biomolecule of lecithin as a biocompatible surfactant to overcome this problem, and successfully prepared water-soluble drug-containing ACP-PLA nanofibers. The lecithin and ACP nanoparticles played important roles in stabilizing water-soluble drug in the electrospinning composite solution. The electrospun drug-containing ACP-PLA nanofibers exhibited fast mineralization in simulated body fluid. The ACP nanoparticles played the key role of seeds in the process of mineralization. Furthermore, the drug-containing ACP-PLA nanofibers exhibited sustained drug release which simultaneously occurred with the in situ mineralization in simulated body fluid. The osteoblast-like (MG63) cells with spreading filopodia were well observed on the as-prepared nanofibrous mats after culturing for 24 hours, indicating a high cytocompatibility. Due to the high biocompatibility, sustained drug release, and fast mineralization, the as-prepared composite nanofibers may have potential applications in water-soluble drug loading and release for tissue engineering.
研究领域:
生物材料
·
药物递送
·
组织工程
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
JACS Au
·
2024
·
IF 8.5
Yaoyun Wu,Yaozhong Cui,Wei Song,Wanqing Wei,Zhizhen He,Jinyang Tao,Dejing Yin,Xiulai Chen,Cong Gao,Jia Liu,Liming Liu,Jing Wu
l-Amino acid oxidase (LAAO) is an important biocatalyst used for synthesizing α-keto acids. LAAO from Rhodococcus opacus (RoLAAO) has a broad substrate spectrum; however, its low total turnover number limits its industrial use. To overcome this, we aimed to employ crystal structure-guided density functional theory calculations and molecular dynamic simulations to investigate the catalytic mechanism. Two key steps were identified: S → [TS1] in step 1 and Int1 → [TS2] in step 2. We reprogrammed the transition states [TS1] and [TS2] to reduce the identified energy barrier and obtain a RoLAAO variant capable of catalyzing 19 kinds of l-amino acids to the corresponding high-value α-keto acids with a high total turnover number, yield (≥95.1 g/L), conversion rate (≥95%), and space-time yields ≥142.7 g/L/d in 12–24 h, in a 5 L reactor. Our results indicated the promising potential of the developed RoLAAO variant for use in the industrial production of α-keto acids while providing a potential catalytic-mechanism-guided protein design strategy to achieve the desired physical and catalytic properties of enzymes.
研究领域:
生物催化
·
酶工程
·
计算化学
·
蛋白质工程
✓ 已验证
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 6
·1684-1696
·
IF 2.137
Xiaoyu Chen,Xiaomeng Wu,Zhongyong Fan,Qinghua Zhao,Qing Liu
The macroinitiator of poly(1,3-trimethylene carbonate) (PTMC) with number-average molecular weight ( ) of 9.6 × 10 3 g mol −1 was synthesized by ring-opening polymerization at 120°C. Then, the novel terpolymer P(TMC- b -( L LA- ran -GA)) consisting of PTMC homopolymer segment attached with various monomer molar ratios of L -lactide ( L LA) and glycolide (GA) random copolymerization block was prepared with about 5.0 × 10 4 g mol −1 by ring-opening polymerization in bulk at 140°C. The tailored molecular structures of P(TMC- b -( L LA- ran -GA)) were characterized by 1 H nuclear magnetic resonance, 13 C NMR, FTIR, and gel permeation chromatography, and chain microstructure analysis was performed in detail with 13 C NMR spectroscopy. The effect of GA units on the thermal and crystallization behaviors, mechanical properties, as well as biodegradability of terpolymers was investigated by differential scanning calorimetry, wide-angle X-ray diffraction, stress-strain measurements, and in vitro tests in comparison with corresponding poly(trimethylene carbonate- block - L -lactide) copolymer P(TMC- b - L LA). The results show that amorphous PTMC segments have a significant effect on condensed state behavior of the terpolymers, and the incorporation of GA units strongly decreases the crystallinity and crystallization ability of L LA segment within terpolymers because of more random L LA-GA sequence and shorter average L LA block length. Meanwhile, the toughness of materials is greatly improved, and in vitro degradation is also accelerated. Peripheral vascular stents were 3D printed for the first time and met the requirements for application. The results show totally biodegradable terpolymers with unique molecular structure, and modifiable properties are promising new biomaterials with advanced performance for biomedical application.
研究领域:
生物材料学
·
高分子化学
·
医学工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yun Liu,Zhina Qiao,Hengwei Zhang,Xian Zhang,Yanan Li,Zhiming Rao
D-tagatose, a functional rare sugar, has physiological effects such as lowering blood sugar and anti-caries. However, research on the industrial production of D-tagatose has primarily focused on Escherichia coli , limiting application in the food field. In this study, a cell factory for efficient synthesis of D-tagatose was constructed using the GRAS strain Corynebacterium glutamicum . Firstly, the key enzyme L-arabinose isomerase from Lactobacillus fermentum was screened. Secondly, by rational design, the optimal mutant D390V/H339P significantly enhanced enzyme activity by 40.65 % and improved thermostability. Subsequently, a dual-enzyme coupled whole-cell system was established by regulating the ratio of β-galactosidase and L-arabinose isomerase by RBS optimization. After optimizing the catalytic system, 101.42 g/L D-tagatose was produced from 400 g/L lactose, and the conversion rate reached 25.12 % in 5 L fermenter, which is the highest yield using lactose as substrate and GRAS strain as chassis cells. This research laid a foundation for the industrial application of food-grade D-tagatose.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Jiayu Zheng,Longxiao Zhou,Qiufen Liu,Senwei Wu,Yuting Peng,Xiujian Zhao,Shouqin Tian
Ratiometric optical thermometry has emerged as a vital platform for non-contact temperature monitoring in industrial and scientific applications, demanding sensors with high sensitivity, non-invasiveness, and spatial resolution. Although zeolitic imidazolate framework-8 (ZIF-8) possesses attractive thermal stability and structural tunability, its limited luminescence intensity hinders practical thermometric applications. Meanwhile, ZIF-L, a two-dimensional (2D) polymorph of ZIF-8 with identical ligands, remains unexplored for temperature sensing. Herein, we propose a dual-modulation strategy synergizing lanthanide valence engineering with MOF phase control to develop a multi-emission center Eu3+/Eu2+ co-doped ZIF-L film (Eu/ZIF-L). By introducing Eu3+ into ZIF-8 precursors, the partial reduction of Eu3+ to Eu2+ under thermal treatment triggers a structural evolution from 3D ZIF-8 to 2D ZIF-L. The resultant Eu/ZIF-L exhibits dual emission peaks at 450 nm (Eu2+) and 495 nm (ligand-centered), with their intensity ratio (I495/I450) demonstrating remarkable linear temperature dependence over 296–373 K and achieving a relative sensitivity of 1.95% K−1. This work pioneers the integration of valence-state manipulation and MOF phase transition for thermometric optimization, providing a novel paradigm to design advanced ratiometric MOF thermometers for industrial thermal management.
✓ 已验证
产品关联
DOI
高影响因子
THERAPEUTIC DRUG MONITORING
·
2025
·
IF 2.4
Yu Hengyi,Hu Qun,Liu Lu,Xiang Dong,Yang Guangjie,Wang Yaqin,Wang Zhen,Liu Dong,Liu Aiguo,Gong Xuepeng
Background: Acute lymphoblastic leukemia (ALL) is the predominant malignancy in pediatric patients. As a crucial constituent of ALL chemotherapy, l -asparaginase is recognized as an integral element of treatment with a threshold concentration of 0.1 IU/mL used in treatment protocols. This study presents a novel liquid chromatography–tandem mass spectrometry method for evaluating plasma l -asparaginase activity in pediatric patients with ALL undergoing pegaspargase therapy. Methods: Initially, an enzyme incubation was conducted using 20 μL of plasma and 100 μL of l -asparagine (0.1 mol/L) at 37°C for 15 minutes. The reaction was stopped by adding sulfosalicylic acid and methanol. After the addition of isotope internal standard l -aspartic 13 C 4 acid, subsequent centrifugation, and dilution, the plasma samples underwent analysis by liquid chromatography–tandem mass spectrometry on a hydrophilic interaction liquid chromatography analytical column. A flow rate of 0.3 mL/min was used during isocratic elution using a mobile phase consisting of methanol–water (95:5, vol/vol) with 0.2% formic acid within a 5-minute run. Results: The calibration curves exhibited excellent linearity ranging from 0.1 to 15 IU/mL, with determination coefficients ( r 2 ) exceeding 0.99. The precision and accuracy ranged from 1% to 7% and from 93% to 110%, respectively. The relative recovery fell within the range of 98%–100%, and the internal standard–normalized matrix effect ranged from 95% to 101%. The stability was satisfactory across various conditions. Conclusions: This method was fully validated and successfully applied to quantify l -asparaginase activity in plasma samples of 15 children with ALL, enabling the monitoring of l -asparaginase activity with mass spectrometry.
研究领域:
临床化学
·
肿瘤学
·
儿科学
·
药代动力学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER
·
2020
·
IF 4.231
Chandra Sekhar Biswas,Arpan Biswas,Massimillano Galluzzi,Mehdihasan I. Shekh,Qiao Wang,Biswajit Ray,Pralay Maiti,Florian J. Stadler
The synthesis of amphiphilic block-copolymers of poly( N -isopropylacrylamide) (PNIPAM) and poly( N -acryloyl- l -phenylalanine methyl ester) (PNALPA) by RAFT polymerization is reported. The polymers were characterized by proton NMR ( 1 H NMR), gel permeation chromatography (GPC), powder X-ray diffraction (XRD), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA) to understand their structure-property relationships. All synthesized polymers show pH triggered release at acidic (pH 5) and biological pH (pH 7.4). Critical micellar concentration also varied systematically depending on the amphiphilic nature of the polymers. Biocompatibilities of the polymers are also determined to check their suitability for biomedical applications.
研究领域:
材料科学
·
生物医学工程
·
高分子化学
✓ 已验证
DOI
高影响因子
NATURE BIOTECHNOLOGY
·
2022
·Vol. 40
·期 11
·1601-1609
·
IF 68.164
Chen Ji,Chen Mengyin,Zhu Ting F.
Mirror-image aptamers made from chirally inverted nucleic acids are nuclease-resistant and exceptionally biostable, opening up opportunities for unique applications. However, the directed evolution and selection of mirror-image aptamers directly from large randomized l -DNA libraries has, to our knowledge, not been demonstrated previously. Here, we developed a ‘mirror-image selection’ scheme for the directed evolution and selection of biostable l -DNA aptamers with a mirror-image DNA polymerase. We performed iterative rounds of enrichment and mirror-image polymerase chain reaction (PCR) amplification of l -DNA sequences that bind native human thrombin, in conjunction with denaturing gradient gel electrophoresis (DGGE) to isolate individual aptamers and l -DNA sequencing-by-synthesis to determine their sequences. Based on the selected l -DNA aptamers, we designed biostable thrombin sensors and inhibitors, which remained functional in physiologically relevant nuclease-rich environments, even in the presence of human serum that rapidly degraded d -DNA aptamers. Mirror-image selection of biostable l -DNA aptamers directly from large randomized l -DNA libraries greatly expands the range of biomolecules that can be targeted, broadening their applications as biostable sensors, therapeutics and basic research tools.
研究领域:
分子生物学
·
生物技术
·
纳米技术
·
治疗学
·
传感器
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 52
·32786-32794
·
IF 3.108
Jingqian Fan,Yuangang Liu,Shibin Wang,Yulu Liu,Siming Li,Ruimin Long,Ran Zhang,Ranjith Kumar Kankala
Layer-by-Layer (LbL) self-assembly of nanocarriers has garnered the interest of researchers for a wide variety of biomedical applications. In this study, we demonstrated the preparation of poly(lactide-co-glycolide) (PLGA)-(poly-L-ornithine (PLO)/fucoidan)4 core–shell nanoparticles (LbL NPs) by a LbL-based self-assembly process, which possessed a mean size of 170 nm. In LbL NPs, a drug carrying PLGA nano-core is coated with alternating PLO and sulfated polysaccharide fucoidan composite films as a shell on the surface. The anti-tumor drug doxorubicin (DOX) loaded into the PLGA core, resulted in better encapsulation efficiency and its in vitro release from LbL NPs demonstrates that this core–shell strategy takes an advantage of its ability to hold the drug cargo and exhibit controlled release. Further, in vitro cell uptake studies by confocal laser scanning microscopy (CLSM) examination in breast tumor cells (MCF-7 cell line) have confirmed that the nanocarriers are successfully internalized and outlined their presence in the cytoplasm after 4 h of incubation. These intracellularly delivered DOX-loaded LbL NPs exhibited significant anti-tumor activity against breast tumor cells. This innovative chemotherapeutic design taking above advantages of successful internalization along with controlled release property signifies as a promising interventional therapeutic delivery system.
研究领域:
药剂学
·
生物材料
·
肿瘤学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 43
·9031-9040
·
IF 6.331
Jilin Zheng,Peng Zhao,Shiying Zhou,Sha Chen,Yi Liang,Fengchun Tian,Jun Zhou,Danqun Huo,Changjun Hou
Integrating metal–organic frameworks (MOFs) of different components or structures together and exploiting them as electrochemical sensors for electrochemical sensing has aroused great interest. Furthermore, the incorporation of noble metals with MOFs is conducive to the improvement of catalytic performance. In this work, Pd@UiO-66-on-ZIF-L nanomaterials were successfully synthesised onto a self-supported flexible carbon cloth (Pd@UiO-66-on-ZIF-L/CC) through a novel strategy called MOF-on-MOF. Then, Au nanoparticles were electrodeposited onto Pd@UiO-66-on-ZIF-L/CC to obtain Au–Pd@UiO-66-on-ZIF-L/CC, which can serve as an excellent electrocatalyst for the reduction of hydrogen peroxide (H2O2). The obtained flower-like Pd@UiO-66-on-ZIF-L/CC hybrid MOF changes the structure of the monomeric MOF alone and adds more attachment sites. The synergy of the bimetals greatly improved the catalytic performance of the as-developed sensor. Electrochemical experiment results show that the proposed sensor based on Au–Pd@UiO-66-on-ZIF-L/CC has an extended linear range from 1 μM to 19.6 mM with a sensitivity of 390 μA mM−1 cm−2, and a low limit of detection (LOD) of 21.2 nM (S/N = 3). Moreover, it has good anti-interference, reproducibility, repeatability and excellent stability. Furthermore, the real-time in situ detection of H2O2 secreted from human adenocarcinomic alveolar basal epithelial cells (A549 cells) was achieved by culturing cells on Au–Pd@UiO-66-on-ZIF-L/CC, which indicates the potential of the sensor for applications in cancer pathology. Both the synthesis strategy and the sensor design provide new methods and ideas for the production of ultrasensitive H2O2 electrochemical sensors.
研究领域:
材料科学
·
电化学
·
生物传感器
·
癌症病理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）