BIOMATERIALS
·
2013
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IF 7.604
Yiyan He,Yu Nie,Li Xie,Hongmei Song,Zhongwei Gu
Reduction-sensitive hyaluronic acid derivatives (HA–SS–COOH) were shielded on the DNA/polyethylenimine (PEI) to construct ternary complexes ( D NA/ P EI/HA– S S–COOH, DPS ternary complexes) with efficient gene transfection. Details studied were conducted to investigation of factors influencing transfection efficiency, including the gene compression by fluorescence resonance energy transfer (FRET) spectrum and the intracellular fate of fluorescent labeled complexes by the confocal laser scanning microscope (CLSM). In the FRET study, DPS complexes were found to enhance condensation of DNA in preparation, while timely loosen gene under exposure to reductive reagent. Similar cellular uptake levels were observed for the designed reduction sensitive complexes and the stable one ( D NA/ P EI/ H A, DPH ternary complexes), but the intracellular process was strikingly different for the two types of complexes. Only DPS showed obvious desired intracellular deshielding and endosomal escape, which contributed to highly efficient gene delivery. After loading with p53 plasmid, DPS complexes achieved significantly up-regulated p53 tumor suppressor gene expression at both mRNA and protein levels, as revealed by quantitative polymerase chain reaction (qPCR) and western blot investigations. Transgene induced apoptosis was evaluated by propidium iodide staining and flow cytometry analysis of cell cycle. Tumor cells transfected by DPS complexes containing p53 gene displayed almost 50% higher suppression in proliferation compared to those untreated cells, accompanied with a 46% elevation in the number of cells at sub-G1 phase and remarkable p53 dependent cell cycle perturbations prior to apoptosis. These results demonstrated that targeted delivery of p53 gene via reduction-sensitive DPS ternary complexes enabled up-regulated cellular p53 mRNA level through the exogenous p53 gene, inducing a significant p53-dependent anti-proliferative effect on tumor cells, which could be effective means of cancer treatment.
研究领域:
生物医学工程
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分子生物学
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癌症研究
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基因治疗
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 46
·18345-18354
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IF 3.069
Gaozhi Bai,Yu Han,Panpeng Du,Zhaoyang Fei,Xian Chen,Zhuxiu Zhang,Jihai Tang,Mifen Cui,Qing Liu,Xu Qiao
A highly efficient solid-amine adsorbent with excellent CO2 capacity from flue gas was successfully prepared using polyethyleneimine (PEI)-impregnated resin HPD450 in this study. The adsorbents were characterized by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), N2 adsorption–desorption, thermogravimetric analysis (TGA) techniques and elemental analysis (EA). The CO2 adsorption behaviors of the adsorbent were studied for their adsorption capacity, kinetics, thermodynamics and stability. The optimal adsorbent (HPD450/PEI-50) exhibited an excellent adsorption capacity of 4.44 mmol g−1 for pure CO2 and 3.62 mmol g−1 for 15 vol% CO2. Moreover, HPD450/PEI-50 retained an average capacity of 3.58 mmol g−1 under flue gas for 90 consecutive adsorption cycles. The adsorption/desorption process of CO2 on HPD450/PEI-50 was investigated using in situ FTIR. The capture of carbon dioxide in the simulated flue gas stream also demonstrated that moisture had a promoting effect on CO2 adsorption. This design of the high-performance HPD450/PEI-50 with a fantastic CO2 capacity could provide an up-and-coming candidate for CO2 capture from flue gas.
研究领域:
环境科学
·
材料科学
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2019
·Vol. 33
·期 5
·e4850
·
IF 3.259
Mingliang Ma,Yuying Yang,Yanyan Liu,Wenting Li,Guopeng Chen,Yong Ma,Ping Lyu,Shunhe Li,Yubao Wang,Guanglei Wu
In this paper, a simple route for palladium (Pd) nanoparticles attached to the surface of hollow magnetic Fe 3 O 4 /P (GMA-DVB)-polyethyleneimine (PEI) microspheres was established. Due to the large amount of imidogen groups and tertiary amine groups presenting in the PEI, Pd 2+ ions could be anchored to the support by complexation with a polyfunctional organic ligand. Thereafter, a magnetic Pd catalyst having a high loading amount and good dispersibility was obtained by reducing Pd 2+ ions. Afterwards, the prepared catalyst was characterized by TEM, SEM, FTIR, XRD, TGA, VSM, and UV–vis in detail. Ultimately, their catalytic activity was evaluated using the reduction of 4-nitrophenol (4-NP). Research showed that the Fe 3 O 4 /P (GMA-DVB)-PEI/Pd catalyst possessed high catalytic performances for the reduction of 4-NP with a conversion rate of 98.43% within 540 s. Furthermore, the catalyst could be easily recovered and reused at least for nine successive cycles.
研究领域:
材料科学
·
催化化学
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Junjie Zhu,Linbo Wu,Zhiyang Bu,Suyun Jie,Bo-Geng Li
Searching for highly efficient and robust CO2 adsorbents is very important for CO2 capture, utilization, and storage. In this study, new polyethylenimine-grafted metal–organic framework (MOF)–polymer composites, abbreviated as PEI@PGD-H, were synthesized via high internal phase emulsion (HIPE) template polymerization of divinylbenzene and glycidyl methacrylate, followed by in situ generation of HKUST-type MOF in the presence of hydrophobic-modified CuO nanoparticles via the reaction of CuO with 1,3,5-benzenetricarboxylic acid and then polyethylenimine (PEI) functionalization via epoxy–amine reaction and amine–metal sites interaction. The coexistence of the hierarchical interconnected porous skeleton of poly(glycidyl methacrylate–divinylbenzene) (PGD), high specific surface area of MOF, and chemical CO2 adsorption of PEI endows PEI@PGD-H with high CO2 adsorption capacity, rate, and selectivity. PEI70@PGD-H shows a CO2/N2 separation factor as high as 76 and CO2 adsorption capacities of 4.3, 3.0, and 1.8 mmol of CO2/g from pure CO2, simulated flue gas, and air, respectively. After 20 CO2 adsorption–desorption cycles, the adsorbent still retained a high and stable CO2 capture capacity over 2.8 mmol of CO2/g for simulated flue gas. The adsorbent also displays reasonably good thermal stability, excellent water endurance, low desorption energy, and easy shaping. The desorption heat is as low as 48 kJ/mol of CO2. These features make PEI@PGD-H highly efficient, robust, and low-energy CO2 adsorbents for practical application in CO2 capture.
研究领域:
材料科学
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环境科学
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PROGRESS IN ORGANIC COATINGS
·
2023
·
IF 6.6
Zhuohan Chen,Xiaojing Su,Kunquan Li,Rongxuan Chen,Zhixuan Lai,Xuanjun Li,Shengye Chen,Wenjian Wu
Superhydrophobic materials possess prospective applications of oily wastewater treatment due to their high efficiency and easy operation. Nevertheless, most superhydrophobic materials are difficult to separate oil-water emulsion, especially for highly viscous water-in-crude oil emulsion. Herein, a superhydrophobic wood with excellent Joule heat and demulsification was proposed by chemically treating the natural wood and subsequent coating of the carboxyl-modified carbon nanotubes (cCNTs) for providing conductive pathways, polyethylenimide (PEI) for supporting demulsification and polydimethylsiloxane (PDMS) for hydrophobic modification. The fabricated superhydrophobic PDMS@PEI-cCNTs wood displayed a water contact angle of 155 o and great compression property with a nearly undamaged structure at a strain of 40 % after 50 compression tests, which could separate and recover oil from the oil-water mixture via absorption-extrusion process. Besides, due to the filtration and electrostatic interaction of the PDMS@PEI-cCNTs wood, the water-in-oil emulsion was demulsified quickly with a separation efficiency greater than 99.7 %. Meanwhile, the PDMS@PEI-cCNTs wood showed a good Joule heat capability, and the surface temperature reached 160 °C at 35 V of voltage within 120 s, which largely reduced the viscosity of water-in-crude oil emulsion. Thus, the PDMS@PEI-cCNTs wood was successful in separating the water-in-crude oil emulsion via Joule heat capability with a separation rate of 4.33 × 10 4 kg·m −3 ·h −1 and a separation efficiency of 93.4 %. The material developed in this study has wide range of applications in treating the stable crude oil-water emulsion with high viscosity.
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JOURNAL OF SOLID STATE CHEMISTRY
·
2022
·
IF 3.656
Yunyun Lei,Xiaoxun Liu,Jilin Zhang,Zi Dai,Xiaoya Zhao,Guixia Liu
Metal-organic framework materials (MOFs) have shown efficient adsorption performance in organic dye removal . However, its relatively poor capacity and slow adsorption kinetics often limit its further application. Herein, polyethyleneimine (PEI) was cross-linked and encapsulated in ZIF-8 to prepare novel composite (ZIF-8@PEI-CC) by using cyanuric chloride (CC). It was found that the composite exhibited excellent adsorption performance for methyl orange (MO). Compared with ZIF-8 (183 mg g −1 ), the adsorption capacity (710 mg g −1 ) was significantly enhanced, and it was easier to separate than ZIF-8. Moreover, it shows a faster adsorption rate than ZIF-8. The adsorption kinetics of the ZIF-8@PEI-CC is more consistent with the pseudo-secondary kinetic model . Isotherm of the ZIF-8@PEI-CC is more in line with the Langmuir model. In addition, the ZIF-8@PEI-CC shows high adsorption capacity in a wide pH range (4-11). Therefore, the synthesized composite ZIF-8@PEI-CC has great potential as an effective adsorbent for removing MO.
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CATALYSIS LETTERS
·
2023
·
IF 2.8
Xu Hongpeng,Zhao Changlu,Hu Tingting,Huang Qi,Jin Bo,Guo Ya,Peng Rufang
Ammonium perchlorate (AP) is a commonly used oxidant for rocket solid propellants. To control the thermal decomposition of AP, a new PEI-imidazole derivative (PEI-ICA) was synthesized. With the combination of Cu compound, the heat decomposition behavior of the AP’s composites was tuned, and the activation energy was also reduced. To explore the potential reason for the catalytic effect, a series of measurements were also carried out, which indicates the electrostatic self-assembly of PEI-ICA and its combination with Cu compound should be responsible for the different heat decomposition behavior of AP. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qingdi Kong,Xiaojie Zhang,Kangrui Ma,Yuefa Gong,Hong Peng,Wei Qi
It is a challenging task to remove heavy metal ions efficiently from the wastewater containing high concentrations of complexants. In this work, a novel PEI-modified chitosan/activated carbon composite adsorbent (PCA) was prepared and applied to the removal of Cu(II) from pyrophosphate plating rinsing wastewater. The main species of Cu(II) in the pyrophosphate wastewater was [Cu(HP 2 O 7 ) 2 ] 4− or [Cu(P 2 O 7 ) 2 ] 6− , which were denoted as [Cu(II)-PP] anions. The maximum adsorption capacity of PCA for Cu(II) reached 1.41 mmol g −1 under the condition of pH = 8 and molar ratio of pyrophosphate to Cu(II) = 4:1. The adsorption kinetic behavior of Cu(II) on PCA followed the Elovich model best and PCA attained adsorption equilibrium within 36 h. The thermodynamic studies showed that the adsorption process of Cu(II) by PCA was endothermic and spontaneous. The PCA fixed bed column was used to remove Cu(II) from simulated pyrophosphate plating rinsing wastewater. After three consecutive adsorption-desorption cycles, the adsorption performance, hydraulic conductivity , and mechanical stability of PCA column did not decrease. The FTIR and XPS analysis results indicated that [Cu(II)-PP] anions can be adsorbed on PCA by electrostatic attraction with protonated amine groups or coordination with the amine groups of PCA via ligand substitution.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 18
·10743
·
IF 6.208
Teng Wang,Xi Hu,Yujun Yang,Qing Wu,Chengdian He,Xiong He,Zhenyu Wang,Xiang Mao
Metal-based enzyme mimics are considered to be acceptable agents in terms of their biomedical and biological properties; among them, iron oxides (Fe3O4) are treated as basement in fabricating heterogeneous composites through variable valency integrations. In this work, we have established a facile approach for constructing Fe3O4@Ag composite through assembling Fe3O4and Ag together via polyethyleneimine ethylenediamine (PEI) linkages. The obtained Fe3O4@PEI@Ag structure conveys several hundred nanometers (~150 nm). The absorption peak at 652 nm is utilized for confirming the peroxidase-like activity of Fe3O4@PEI@Ag structure by catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The Michaelis–Menten parameters (Km) of 1.192 mM and 0.302 mM show the higher catalytic activity and strong affinity toward H2O2and TMB, respectively. The maximum velocity (Vmax) value of 1.299 × 10−7M∙s−1and 1.163 × 10−7M∙s−1confirm the efficiency of Fe3O4@PEI@Ag structure. The biocompatibility illustrates almost 100% cell viability. Being treated as one simple colorimetric sensor, it shows relative selectivity and sensitivity toward the detection of glucose based on glucose oxidase. By using indocyanine green (ICG) molecule as an additional factor, a remarkable temperature elevation is observed in Fe3O4@PEI@Ag@ICG with increments of 21.6 °C, and the absorption peak is nearby 870 nm. This implies that the multifunctional Fe3O4@PEI@Ag structure could be an alternative substrate for formatting acceptable agents in biomedicine and biotechnology with enzymatic and photothermal properties.Keywords:Fe3O4@PEI@Ag;assembling process;enzymatic;catalytic performance;photothermal property
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Journal of Materials Chemistry B
·
2022
·Vol. 11
·期 2
·335-344
·
IF 7.571
Yuqing Lei,Jiajia Xu,Mengqi Pan,Yadong Chen,Xiaojun Li,Weipu Zhu,Chang Shu,Tianxiang Fang,Hongbing Liao,Qiaojie Luo,Xiaodong Li
Dentin bonding is the most common form of human tissue repair among tissue-biomaterial adhesions, concerning billions of people's oral health worldwide. However, insufficient adhesive infiltration in the demineralized dentin matrix (DDM) always produces numerous defects in the bonding interface termed the hybrid layer, which causes high levels of bacteria-related secondary dental diseases, and less than 50% of the bonding lasts more than 5 years. Therefore, it is urgent and vital to construct an antibacterial low-defect hybrid layer to solve the durability-related problems. A DDM with a hydrogel-like surface formed by the hydration of highly-anionic non-collagenous proteins (NCPs) is firstly used as a template to electrostatically assemble polyethyleneimine (PEI). The formation of a stable antibacterial polyelectrolyte complex of PEI/NCPs rapidly eliminates NCP hydration capacity and significantly improves the infiltration of various adhesives. Simultaneously, both the PEI during the assembly and the PEI-assembled DDM can directly destroy a biofilm of S. Mutans on the DDM. Consequently, a long-term antibacterial and low-defect hybrid layer is successfully created, which greatly improves the bonding effectiveness. This helps to improve the clinical treatment of bacteria-based dental diseases and the tooth-restoration repair effect and prevent secondary dental diseases, having significance in clinical dentistry and providing insights for other tissue-biomaterial adhesions.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 5
·1-10
·
IF 6.408
Ding Jinglei,Zhang Wenqi,Xue Fengying,Sun Yu,Yan Quizhu,Chen Yanwei,Shan Guiye
Gold nanoclusters (AuNCs) are widely used in the fluorescence detection of biomolecules in human serum due to their good fluorescence properties, low toxicity, and better biocompatibility. However, the weak fluorescence intensity of AuNCs limits the fluorescence detection of molecules within a wide concentration range. It is reported that coating AuNCs in ZIF-8 with adjustable pore size can effectively improve the fluorescence intensity of AuNCs and broaden the detection range. And the AuNCs wrapped in the gaps of ZIF-8 can prevent the fluorescence quenching caused by the aggregation of AuNCs. However, ZIF-8 has high crystallinity, poor dispersion, and easy deposition, which reduces the fluorescence stability of the detection system and affects the detection. Based on the above research, the highly hydrophilic polymer PEI was modified to the surface of ZIF-8, and a kind of nanocomposite material AuNCs/ChOx@ZIF-8/PEI was obtained by co-encapsulating AuNCs prepared with glutathione as a ligand and cholesterol oxidase (ChOx) into ZIF-8 modified with PEI. The composite material emits strong red light at 650 nm under the excitation of 395-nm light, and the system can sensitively detect cholesterol (Chol) in human serum. Compared with other materials, the PEI-modified composite has better solubility and stability, so the detection effect of Chol is better. Encapsulation of ChOx in the ZIF-8 shell can protect the enzyme and increase the local concentration of ChOx, thereby speeding up the reaction rate. Compared with free AuNCs/ChOx, the quenching rate of AuNCs/ChOx@ZIF-8/PEI system is doubled. Secondly, the addition of Fe 2+ to the detection process results in higher quenching rate and detection sensitivity. The system can detect Chol in the concentration range 0.1–2.4 μM, with a detection limit of 0.073 μM. The determination is a fast and sensitive strategy. In addition, the practicability of this assay in the detection of Chol in human serum has been verified. Due to its selectivity and sensitivity, it has potential application value in clinical diagnosis. Graphical abstract
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Ge Yang,Yanjiao Wang,Mengling Sun,Panpan Xu,Chunzheng Wang,Ke Huang,Heqing Jiang,Svetlana Mintova,Hailing Guo
The amino-modification of the filler material is one of the most common strategies for optimization of the performance of mixed matrix membranes, aiming to enhance the interactions between the filler and the organic matrix and accelerate the adsorption of gas molecules by the filler. Herein, we found that the in-situ introduction of amino groups into the HKUST-1 filler would have negative effects on the gas separation performance of the MMMs following the “diffusion-adsorption coupling control” mechanism, which suggests that using MOFs fillers with large aperture size to accelerate the diffusion of small H 2 molecules and slow the diffusion of polar CO 2 molecules through the adsorption-desorption process. The NH 2 -HKUST-1 filler caused the blockage and loss of the pore structure , and the interfacial interactions in the PEI-based mixed matrix membranes were not improved. Therefore, the in-situ synthesized NH 2 -HKUST-1 did not support H 2 /CO 2 separation. This work further investigates the possible “diffusion-adsorption coupling control” mechanism of fillers with large aperture size and contributes to the development of MMMs materials.
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