Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Wang Min,Guo Yi,Deng Zexing,Xu Peng
The development of bioactive materials with good mechanical properties and promotion of stem cell osteogenic differentiation has important application prospects in bone tissue engineering. In this paper, we designed a novel organic‒inorganic composite hydrogel (FPIGP@BGN-Sr) utilizing diacrylated F127 (DA-PF127), β-glycerophosphate-modified polyitaconate (PIGP) and strontium-doped bioactive glass nanoparticles (BGN-Sr) through free radical polymerization and coordination interactions and then evaluated its promoting effect on the osteogenic differentiation of mouse embryonic mesenchymal stem cells in detail. The results showed that the FPIGP@BGN-Sr hydrogel exhibited a controlled storage modulus by changing the amount of BGN-Sr. Notably, the FPIGP@BGN-Sr hydrogel possessed excellent elastic ability with a compressive strain of up to 98.6% and negligible change in mechanical properties after 10 cycles of compression. In addition, the FPIGP@BGN-Sr hydrogel had good cytocompatibility, maintained the activity and proliferation of mouse embryonic mesenchymal stem cells (C3H10T1/2), and effectively enhanced the activity of alkaline phosphatase, osteogenic gene expression and biomineralization ability of the cells. In conclusion, the excellent mechanical properties and osteogenic biological activity of the FPIGP@BGN-Sr hydrogel make it a promising organic‒inorganic composite bioactive material for stem cell-based bone regeneration.
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高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2018
·Vol. 106
·期 11
·2973-2983
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IF 3.231
Yimeng Niu,Xiaofang Chen,Danyu Yao,Ge Peng,Haifeng Liu,Yubo Fan
Nerve regeneration and function recovery remain challenges for tissue engineering. The application of suitable scaffold in tissue engineering has been demonstrated to be able to enhance nerve regeneration and differentiation. However, a desired scaffold must meet the requirements of good cytocompatibility and high electrical conductivity simultaneously. In this study, a conductive film composed of SF and graphene was successfully fabricated, which was applied to evaluate its effect on the neural differentiation of iPSCs. The conductive film was found to enhance the differentiation of iPSCs toward neurons. In addition, the differentiation was enhanced with graphene contents and highest value was obtained at graphene content of 4%. Thus, the results in this study suggested that 4% G/SF film might be a suitable biomaterial scaffold for application in neural regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2973–2983, 2018.
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COMPOSITES PART B-ENGINEERING
·
2018
·
IF 4.92
Fanrong Meng,Guoqing Wang,Xueyu Du,Zhifen Wang,Shuying Xu,Yucang Zhang
Banana pseudo-stems fibers were novel extracted via atmospheric liquefaction combined with TEMPO-mediated oxidation . Cellulose nanofibers (CNF) and nanocrystals (CNC) were subsequently obtained by high-intensity ultrasonic treatment and acidolysis, respectively. Liquefied residue content as well as its constituent varied with liquefaction time. Scanning electron microscopy and fourier transform infrared demonstrate that the progressive removal of non-cellulosic impurities. Morphological studies exhibit that CNF present a range of 3∼10 nm in diameter and 140∼690 nm in length, while CNC have average diameter of 3.5 ± 1.6 nm and a length of 93.3 ± 37.6 nm. XRD analysis indicates that the CNCs have a high crystallinity (95.6%). The thermal stability of the fibers was measured by thermogravimetric analysis . The structures and properties of CNF and CNC give it potential to be used in reinforcing polymeric matrix materials .
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 44
·e2513015122
·
IF 9.1
Anqi Li,Dong Tong,Benjamin Ohlstein,Zheng Guo
Recent work suggests that asymmetric segregation of preexisting and newly synthesized canonical histone 3.1 (H3.1), but not variant histone 3.3A (H3.3A), plays an important role in the asymmetric outcome of Drosophila germline stem cell (GSC) and intestinal stem cell (ISC) divisions. However, this finding relied on the Gal4/UAS system and Flp-out technology to swap expression of exogenous fluorescent proteins—an approach that decouples H3.1 expression from the cell cycle and may be prone to artifacts. Here, by photoswitching photoconvertible H3-Dendra2 proteins expressed under their native controls in living flies, we find that preexisting and newly synthesized H3.1 and H3.3A are symmetrically segregated in dividing ISCs and somatic cyst stem cells (CySCs) in male testes. While H3.3A was found to be symmetrically segregated during GSC divisions, analysis of the H3.1 segregation in GSCs was complicated by our unexpected finding that H3.1-Dendra2 expressed under its normal control within the context of a histone transgene is not detectable in GSCs, owing to regulatory elements in the 3’UTR that mediate translational repression by the RNA-binding proteins Nanos and Pumilio. Nevertheless, when this repression is obviated by using a 3’UTR devoid of such elements, H3.1-Dendra2 is expressed in GSCs and symmetrically segregated without affecting lineage outcomes. We obtained similar results when the coding sequence of H3.3A was edited to encode H3.1 in the context of an H3.3A-Dendra2 knock-in allele. Our findings thus challenge the prevailing model of asymmetric histone segregation and reveal an unexpected molecular mechanism of posttranscriptional repression of H3.1 in GSCs.
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Biomaterials Advances
·
2025
·
IF 6
Bo Li,Chengwei Wang,Junbo Jiang,Miaomiao Zhang,Shixin Xu,Jinxin Zhang,Zhiyi Gao,Jierui Li,Li Xie,Wen Zhao
Wound healing is a complex and critical physiological process. Traditional cell transplantation strategies often fail to ensure long-term cell survival owing to the detrimental effects of the wound microenvironment, such as acidity, hypoxia, and nutrient deficiency. Therefore, encapsulating cells within microcapsules before transplantation to the wound site is highly desirable. In this work, a core–shell conductive nanofiber membrane with favorable mechanical properties was integrated with conductive microcapsules loaded with adipose-derived mesenchymal stem cells (ADSCs) to create an ADSCs delivery system, resulting in a microencapsulated ADSCs/conductive nanofiber composite dressing. Both the conductive nanofibers and microcapsule materials exhibited a porous, breathable structure. The core–shell conductive nanofibers showed excellent porosity, hydrophilicity, and in vitro degradation properties. Furthermore, the composite dressing exhibited good biocompatibility and antibacterial properties. Electrical stimulation in vitro effectively promoted the paracrine activity of the ADSCs within the microcapsules, preserving their viability and stemness. In vivo experiments revealed that the composite dressing group exhibited a significantly enhanced wound contraction compared to the control group, with the neoepidermis at the wound site more closely resembling the normal skin structure, displaying an increased and well-organized collagen deposition. Moreover, the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was reduced while microvessel formation increased, thereby accelerating wound healing. In conclusion, the microencapsulated ADSCs/conductive nanofiber composite dressing possessed excellent conductivity, antibacterial properties, biocompatibility, and inflammation-modulating capabilities, endowing it with significant research value in tissue engineering as an innovative wound repair system.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Simiao Yu,Chenyu Liu,Yongxin Pan,Zhangrong Lou,Weihong Qiao
To address the critical challenges of low cell retention and poor survival in neural stem cell (NSC) transplantation for cerebral palsy, we developed an injectable conductive hydrogel (TT-gel) through dynamic silver–sulfur/Schiff base cross-linking of thiolate oxidized sodium alginate and silk fibroin. The optimized TT-gel demonstrated ideal rheological properties (recovery yield in 40 s > 95%) and electrical conductivity (1.47 ± 0.01 S/m), enabling effective 3D NSC culture with high viability (93.8 ± 1.6% at 5 days) while supporting multilineage differentiation. In a rat cerebral palsy (CP) model, NSC delivery via TT-gel achieved significantly promoted substantial functional recovery, evidenced by 2.6-fold improvement in rotarod endurance and 81.3% reduction in water maze escape latency. Proteomics and histological analysis confirmed enhanced neuroregeneration, showing greater neuronal density and elevated synaptic plasticity markers in treated animals. The therapeutic efficacy of TT-gel stems from its unique dynamic scaffold properties and injectable capability, combined with its electroconductive nature that promotes neuronal differentiation. This multifunctional design collectively addresses the key limitations of conventional NSC transplantation approaches, highlighting its significant clinical potential for CP treatment.
研究领域:
生物医学工程
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神经科学
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再生医学
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生物材料
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干细胞治疗
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yang Li,Jun Hou,Jun Guo,Periyasamy Soodamani,Yan Li,Zijun Yang,Yujiao Zhu,Changhao Zhu,Qianqian Zheng
Lithium is a critical strategic while lithium extraction faced with challenges such as slow kinetics and high-water consumption. What's more, high salinity brine has interfering ions hindering the adsorption process. To tackle these problems, a pine stem-inspired tri-functional lithium titanate (Li 4 Ti 5 O 12 , LTO)/ Carbonized aerogel (CA) evaporator (CA@HTO) was synthesized as the key component and integrated into polyethylene (PE) foam platform to create the tri-functional integrated solar evaporator system. On the one hand, the pine xylem-inspired designed aerogel increases water flux, and enriches local Li + supply within aerogel micro channels. On the other, the evaporator shows over 95% solar energy harvesting efficiency to elevate local temperatures, significantly enhancing the endothermic Li + extraction process of lithium-ion sieve (LIS) and solar steam generation. The system doubles the Li + recovery capacity (increasing from 12.66 to 29.12 mg g −1 ) under one-sun illumination, with adsorption kinetics reaching saturation within 6 h—twice the rate observed at 280 K (typical salt-lake temperatures). What's more, the phloem-inspired structure aerogel benefits the directional salt crystallization, which is realized by system design through Marangoni effect. Outdoor solar-powered experiments confirmed the feasibility of stable lithium recovery (>12 mg g −1 ) directly from natural hypersaline salt-lake brines, coupled with self-sustaining water recycling for Li + elution. Salt collection efficiency reaches up to 0.27 kg m −2 h −1 . This work presents an integrated solution for sustainable multiple resources recovery with near-zero water and carbon consumption, contributing to the global target of carbon neutrality.
研究领域:
材料科学
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环境工程
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可再生能源
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可持续资源回收
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纳米材料
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Nanomaterials
·
2022
·Vol. 12
·期 5
·808
·
IF 5.719
Jingjing Fu,Feng Chen,Huihui Chai,Lixia Gao,Xiaohui Lv,Ling Yu
A[email protected]fabric ([email protected]) hybrid scaffold with tailored micropore structures was fabricated by lyophilizing, using gelatin to support cells and the NWF matrix as a frame to enforce the mechanical stability of gelatin. By freezing the gelatin and NWF hybrid in liquid nitrogen and subsequently lyophilizing and crosslinking the process, the[email protected]scaffold was prepared to support cell growth and promote cell aggregation and spheroids’ formation. The results indicated that by tuning the lyophilizing temperature, the micropore size on the gelatin could be tailored. Consequently, tumor spheroids can be formed on[email protected]scaffolds with honeycomb-like pores around 10 µm. The cell spheroids formed on the tailored[email protected]scaffold were characterized in cancer stem cell (CSC)-associated gene expression, chemotherapy drug sensitivity, and motility. It was found that the expression of the CSC-associated biomarkers SOX2, OCT4, and ALDH1A1 in gene and protein levels in DU 145 cell spheres formed on[email protected]scaffolds were significantly higher than in those cells grown as monolayers. Moreover, cells isolated from spheroids grown on[email protected]scaffold showed higher drug resistance and motility. Tumor spheroids can be formed on a long-term storage scaffold, highlighting the potential of[email protected]as a ready-to-use scaffold for tumor cell sphere generation and culturing.Keywords:tumor spheroid;gelatin;non-woven fabric;lyophilized;cancer stem cell
研究领域:
生物材料
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组织工程
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癌症生物学
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高影响因子
EMBO JOURNAL
·
2023
·Vol. 42
·期 6
·e112647
·
IF 11.4
Mingyue Xu,Ye Guo,Min Wang,Xing Luo,Xuning Shen,Zhimin Li,Lei Wang,Weixiang Guo
Neurogenesis in the developing and adult brain is intimately linked to remodeling of cellular metabolism. However, it is still unclear how distinct metabolic programs and energy sources govern neural stem cell (NSC) behavior and subsequent neuronal differentiation. Here, we found that adult mice lacking the mitochondrial urea metabolism enzyme, Arginase-II (Arg-II), exhibited NSC overactivation, thereby leading to accelerated NSC pool depletion and decreased hippocampal neurogenesis over time. Mechanistically, Arg-II deficiency resulted in elevated L-arginine levels and induction of a metabolic shift from glycolysis to oxidative phosphorylation (OXPHOS) caused by impaired attachment of hexokinase-I to mitochondria. Notably, selective inhibition of OXPHOS ameliorated NSC overactivation and restored abnormal neurogenesis in Arg-II deficient mice. Therefore, Arg-II-mediated intracellular L-arginine homeostasis directly influences the metabolic fitness of neural stem cells that is essential to maintain neurogenesis with age.
研究领域:
神经科学
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干细胞生物学
·
代谢
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高影响因子
EMBO REPORTS
·
2020
·Vol. 21
·期 11
·e50283
·
IF 7.497
Mingliang Bai,Dan Ye,Xudong Guo,Jiajie Xi,Nana Liu,Yukang Wu,Wenwen Jia,Guiying Wang,Wen Chen,Guoping Li,Zeyidan Jiapaer,Jiuhong Kang
A microdeletion within human chromosome 5q14.3 has been associated with the occurrence of neurodevelopmental disorders, such as autism and intellectual disability, and MEF2C haploinsufficiency was identified as main cause. Here, we report that a brain-enriched long non-coding RNA, NDIME, is located near the MEF2C locus and is required for normal neural differentiation of mouse embryonic stem cells (mESCs). NDIME interacts with EZH2, the major component of polycomb repressive complex 2 (PRC2), and blocks EZH2-mediated trimethylation of histone H3 lysine 27 (H3K27me3) at the Mef2c promoter, promoting MEF2C transcription. Moreover, the expression levels of both NDIME and MEF2C were strongly downregulated in the hippocampus of a mouse model of autism, and the adeno-associated virus (AAV)-mediated expression of NDIME in the hippocampus of these mice significantly increased MEF2C expression and ameliorated autism-like behaviors. The results of this study reveal an epigenetic mechanism by which NDIME regulates MEF2C transcription and neural differentiation and suggest potential effects and therapeutic approaches of the NDIME/MEF2C axis in autism.
研究领域:
神经科学
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干细胞生物学
·
遗传学
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表观遗传学
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分子生物学
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高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2022
·
IF 4.46
Wei Liu,Zechang Li,Xiaoyu Cui,Fang Luo,Chunyan Zhou,Jiangyu Zhang,Liguo Xing
Despite of the global contamination and ubiquitous exposure to nitenpyram (NIT), little knowledge is available on the adverse effects to human health, with some evidence referring to its genotoxic potency to non-target organisms and esophageal squamous papilloma in rats. Human bone marrow mesenchymal stem cells (hBMSCs) was employed as an in vitro model more relevant to humans to assess the potential genotoxicity of NIT and to understand the underlying mechanisms at cellular and molecular levels. Noncytotoxic concentrations of NIT, 50–2500 μg/mL, dose-dependently elevated micronucleus (MN) and nuclear bud (NB) frequencies to 8.7–29‰ and 15–35‰, respectively. Additional metabolism by rat liver S9 fraction decreased chromosome impairment by 27–52% on MN frequencies and 63–76% on NB frequencies. A commercial NIT product, containing 20% of NIT and 60% of pymetrozine , caused higher cytotoxicity and chromosome impairment in comparison with NIT alone. Expressions of genes responses to DNA damage, ATM , ATR , p53 , p21 , Bax, H2AX , and GADD45A were disturbed by NIT treatment. Reactive oxygen species (ROS) amount and superoxide dismutase (SOD) activity were enhanced by NIT. Comet assay showed that lower concentrations of NIT, 12.5–100 μg/mL, induced the DNA damage. Transcriptomic analysis identified 468 differentially expressed genes ( p < 0.05, |log2(Foldchange)| ≥ 1), from which 22 pathways were enriched. Multiple affected pathways were related to cancer including viral carcinogenesis and bladder cancer . NIT may produce genotoxicity via inducing oxidative stress and deregulating PI3K/Akt, AMPK and mTOR signaling pathways, associated with carcinogenetic potency. While environmental levels of NIT alone may pose little risk to human health, attention should be paid to the health risk arose from the synergistic or additive effects that may exist among NEOs and other types of pesticides.
研究领域:
毒理学
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细胞生物学
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分子生物学
·
基因组学
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高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Jinjin Lu,Rui Li,Shilei Ni,Yuandong Xie,Xinchen Liu,Kai Zhang,Yi Li
Human dental pulp stem cells (hDPSCs) have been a focus of pulp regeneration research because of their excellent odontogenic potential and availability. Applying the odontoblastic differentiation of hDPSCs to tooth regeneration has been challenging. Metformin-based carbon nanodots (MCDs) were synthesized and characterized to investigate their effects in vitro on odontoblastic hDPSC differentiation and the underlying mechanism. MCDs were synthesized by a hydrothermal treatment method and characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. The biocompatibility and fluorescence properties of the MCDs in Dulbecco’s modified Eagle’s medium high-glucose culture medium and the in vitro odontogenic potential and related mechanism of the bioactive nanomaterial was explored. TEM images showed that MCDs were spherical in shape with a size of approximately 5.9 nm. MCDs showed biological safety in cell viability, apoptosis, and fluorescence labelling ability at a concentration up to 200 μg/ml in vitro . The presence of MCDs facilitated high-efficiency odontogenic differentiation of hDPSCs by promoting odontogenic gene and protein expression. Moreover, MCDs promoted odontoblastic hDPSC differentiation via autophagy. MCDs are capable of activating autophagy and enhancing the odontogenic differentiation of hDPSCs by upregulating odontoblast gene marker (DMP1, DSPP, RUNX2, and SP7) and protein (DSPP and DMP1) expression.
研究领域:
牙齿再生
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纳米技术
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干细胞生物学
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分子生物学
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PubMed
高影响因子
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