Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 7
·1482-1497
·
IF 4.543
Xiaobing Chen,Jun Yang,Hong Liang,Qian Jiang,Bowen Ke,Yu Nie
Integrating the advantages of artificial vectors with bioinspired strategies has opened interesting perspectives in the design of gene delivery systems. Herein, disulfide modified, self-assembled lipopeptides with arginine-rich periphery are demonstrated as gene vectors (RLS). It is surprising that these carriers achieved excellent gene transfection efficacy (up to 380-fold higher than PEI) in different cell lines (HeLa and B16 cells) at relatively low N/P (∼10) ratios, compared to the analog lipopeptides without disulfide bonds (RL, N/P 40). As shown from the morphologies observed by transmission electronic microscopy and surface charge detection by dynamic light scattering, the existence of disulfide bonds may influence the configuration/conformation of the RLS assembly with decreased zeta potential (+27.2 mV), compared to that of the analogs, RL (+46.5 mV). Correspondingly, RLS/DNA complexes showed relatively lower zeta potentials than those of RL/DNA complexes at different N/P ratios. Under the transfection conditions, RLS/DNA complexes (N/P 10) showed even less cellular uptake than that of RL/DNA complexes (N/P 40), and no difference was detected in buffering effect and endosomal escape between RLS and RL complexes. Examination by gel electrophoresis and fluorescence resonance energy transfer (FRET) images in cell culture conditions confirmed that RLS lipoplexes could effectively liberate DNA. Thus, the higher gene transfection efficiency of disulfide modified vectors might be mainly attributed to the cleavage of disulfide bonds; the rapid release of DNA and the superiority of the design was closely related to the reductive conditions in the cells.
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高影响因子
FOOD HYDROCOLLOIDS
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2026
·
IF 13.8
Jingnan Zang,Mingmin Qing,Yujie Chi,Yuan Chi
Egg white gel has adverse effects due to the heat induced aggregation of egg white solution, while the specific role of lysozyme during the heat-induced aggregation and subsequent gelation stages is still unclear. In this paper, the role of lysozyme in the process of thermal induced aggregation and thermal gel formation of egg white and the mechanism of its interaction with Arg/Lys were studied. The results showed that the absence of lysozyme had higher turbidity and average particle size of heat induced egg white, water holding capacity of gel and the proportion of bound water than that of lysozyme in the presence of lysozyme, which was due to the weakening of hydrophobic interaction and disulfide bond, as well as the formation of pore structure. Lysozyme and arginine tend to form a uniform and dense gel network, showing higher hardness, chewiness and water retention. On the contrary, lysine and lysozyme tend to form a fibrous porous gel network intertwined with each other, which has relatively low water holding capacity but high hardness. This study provides multi-dimensional insights into the formation process and cross-linking mode of lysozyme in gel network, reveals the mechanism of Arg/Lys differential regulation of gel structure and performance through different paths, and provides a theoretical basis for improving the quality of egg white gel after heat treatment.
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Nanoscale
·
2017
·Vol. 9
·期 32
·11545-11552
·
IF 7.367
Wenjing Lu,Yifang Gao,Yuan Jiao,Shaomin Shuang,Chenzhong Li,Chuan Dong
A new visual fluorescent probe based on carbon nano-dots (CNDs) has been facilely synthesized via one step microwave-assisted pyrolysis and utilized for sequential detection of arginine (Arg) and Cu2+ by fluorescent and colorimetric dual-readout assay. The fluorescence of CNDs can be effectively quenched by Arg, and recovered upon addition of Cu2+ due to the competitive binding of Arg and Cu2+ that leads Arg to escape from the surface of CNDs. The probe displayed high sensitivity and selectivity toward Arg and Cu2+ over other analytes with a low detection limit of 0.26 μM and 0.17 μM, respectively. Meanwhile, the CNDs can also give dual responsive signals of a visible color change (yellow–pink–light yellow). According to this phenomenon, an “AND” logic gate based on the novel CNDs has been constructed. More importantly, the probe was also extended to cellular imaging. The proposed method was simple with ease of operation, which demonstrated great potential in bio-sensing, disease diagnosis or environmental monitoring.
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MOLECULES
·
2018
·Vol. 23
·期 12
·3377
·
IF 3.098
Wen Nie,Ke-zhou Cai,Yu-zhu Li,Shuo Zhang,Yu Wang,Jie Guo,Cong-gui Chen,Bao-cai Xu
(1) Background: Amino acids and carbohydrates are widely used as additives in the food industry. These compounds have been proven to be an influencing factor in the production of chemical carcinogenic compounds polycyclic aromatic hydrocarbons (PAHs). However, the effect of the properties of the amino acids and carbohydrates on the production of PAHs is still little known. (2) Methods: We added different (i) R groups (the R group represents an aldehyde group in a glucose molecule or a ketone group in a fructose molecule); (ii) molecular weight carbohydrates; (iii) polarities, and (iv) acid-base amino acids to pork sausages. The effects of the molecular properties of carbohydrates and amino acids on the formation of PAHs in grilled pork sausages were investigated. (3) Results: The results showed that a grilled sausage with aldehyde-basedd-glucose was capable of producing more PAHs than a sausage with keto-basedd-fructose. A higher PAH content was determined in the grilled pork sausage when the smaller molecular weight,d-glucose, was added compared with the sausage where the larger molecular weight, 4-(α-d-glucosido)-d-glucose and cellulose were added. The addition of basic amino acids (l-lysine,l-arginine) was capable of producing more PAHs compared with the addition of acidic amino acids (l-glutamic acid,l-aspartate). When amino acid containing a benzene ring was added, a smaller volume of PAHs was produced compared with the addition of other amino acids. (4) Conclusions: Our study suggests that systematic consideration of molecule properties is necessary when using food additives (amino acids and carbohydrates) for food processing.Keywords:amino acids;carbohydrates;acidity;polarity;molecular weight
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Zimeng Kong,Yajun Chen,Tian Ye,Jinling Hao,Haoran Shi,Fenghao Hao,Ran Zheng,Lijun Qian
In this study, cellulose nanofibers (CNFs) were utilized as a synergistic agent, and combined with phytic acid arginine salt (PaArg) via blending and bonding. The effects of these different binding techniques of CNFs and PaArg on the flame retardant and mechanical properties of poly (butylene succinate) (PBS) were explored. The results indicated that both blended and bonded CNFs and PaArg enabled PBS composites to achieve a UL 94 V-0 rating, with the limiting oxygen index (LOI) value of the composite exceeding 28 %. When 20.5 % PaArg and 4.5 % CNFs were blended into PBS, the peak heat release rate (PHRR) of the composite decreased by 44.7 % compared to pure PBS. When the same content of hydrogen-bonded PaArg and CNFs(20.5PaArg-4.5CNFs) were added into PBS, the PHRR of the composite (PBS/20.5PaArg-4.5CNFs) reduced by 45.7 %. And it also exhibited a smaller fire growth index (FGI), enhancing the fire safety of the PBS composite. The incorporation of CNFs also improved the bending strength and impact strength of PBS. Specifically, the hydrogen bond between PaArg and CNFs demonstrated superior binding effects. Compared to the PBS/25PaArg composite, the bending strength and impact strength of the PBS/20.5PaArg-4.5CNFs composite were increased by 41.9 % and 82.1 %, respectively.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xiangchen Su,Xinrong Geng,Fang Li,Mingzhu Song,Ruizhen Lv,Yifei Zhang,Jiayu Yuan,Jia Dong,Yijie Shi,Liang Zhao
Due to the high-glucose environment of diabetic wounds, a significant proliferation of bacteria can occur, resulting in an inflammatory response that extends the inflammatory phase of the wound, thereby complicating the healing process in diabetic wounds. Eliminating the proliferation of bacteria plays a crucial role in promoting the healing of diabetic wounds. Under near-infrared (NIR) laser irradiation, l -arginine-modified natural product puerarin (Pue)-derived carbon nanoparticles ( l -Arg-CNP) not only exhibited excellent photothermal effects but also produced reactive oxygen species (ROS) to react with l -arginine ( l -Arg) for producing Nitric Oxide (NO), thus contributing to a synergistic antibacterial therapy in diabetic wound. At the same time, l -Arg-CNP retained Pue’s original characteristics to promote cell proliferation and angiogenesis. Following the loading of l -Arg-CNP into microneedle patches ( l -Arg-CNP@MN), it can deliver them into the deeper wound, effectively killing bacteria, reducing inflammatory infiltration, and promoting neovascularization at the wound site. It offers an effective therapeutic strategy for treating diabetic wound healing.
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Journal of the Iranian Chemical Society
·
2025
·
IF 2.3
Yin Dawei,Chen Jiabao,Liu Yuting,Dang Chi,Zhou Yi,Zhang Zixu,Zheng Ruilin
Glutamic acid (Glu), aspartic acid (Asp), arginine (Arg) and ascorbic acid (AA) play an important role in the body’s protein anabolism, promoting tissue healing, improving disease resistance, as well as promoting bone growth, improving malnutrition, antioxidant and so on. Therefore, rapid detection of Glu, Asp, Arg and AA is of great significance. 2-methoxy-4-(((5-phenyl-1,3,4-thiadiazol-2-yl)imino)methyl)phenol (Probe A) was synthesized with deep eutectic solvent as catalyst under mild conditions. The Probe A was applied to the detection of Glu, Asp, Arg and AA, and the results showed that Probe A could well identify Glu, Asp, Arg and AA. Glu, Arg and AA are well recognized at pH = 6–12. Asp is well recognized at pH = 7–12. The binding ratios between Probe A and Glu, Asp, Arg, AA were 2:3, 2:3, 1:2 and 2:3, respectively. The association constants were 9.59 × 10 3 M −1 , 7.34 × 10 3 M −1 , 4.46 × 10 4 M −1 , 1.69 × 10 4 M −1 , respectively. The detection limits were 3.33 × 10 –5 M, 1.62 × 10 –5 M, 2.22 × 10 –4 M, and 1.69 × 10 –5 M, respectively. The probe solution quickly became colorless after adding Glu, Asp and AA, and orange-red after adding Arg. The identification mechanism of Probe A with Glu, Asp, Arg and AA was obtained by infrared titration and nuclear magnetic titration. This method provides an important way for rapid detection of Glu, Asp, Arg and AA.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Jinru Zhou,Rong Ai,Junjie Weng,Lingfang Li,Chaosheng Zhou,Aijin Ma,Linglin Fu,Yanbo Wang
Shellfish allergy is a crucial public health problem and a severe food safety issue, resulting in an urgent need for an accurate, simple and sensitive method to detect the shellfish allergens in complex food systems. Herein, we have desighed an “on-off-on” fluorescence aptasensor based on fluorescence resonance energy transfer (FRET) using prepared carboxyl groups functionalized CQDs (cCQDs) and graphene oxide (GO) for the detection and quantification of the major shellfish allergen arginine kinase (AK). The FRET aptasensor allowed for the sensitive detection of AK in the range of 0.001–10 μg/mL with the limit of detection (LOD) of 0.14 ng/mL (S/N = 3), and the limit of quantitation (LOQ) of 0.27 ng/mL (S/N = 10). This reliable, accurate and specific aptasensor with user-friendly operation may provide new perspectives in the application of fluorescence technologies for food allergens detection in the field of food safety.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·497
·
IF 5.4
Xie Zexing,Dou Xinkai,Wang Haitong,Liu Yu,Lv Peiwen,Xin Tao,Li Nianlu
At-home monitoring of key blood indicators, including cholesterol and glucose, is vital for point-of-care chronic condition management. In this work, portable kits based on L-arginine modified Cu-CuFe 2 O 4 nanoparticles (Cu-CuFe 2 O 4 @L-Arg NPs) were fabricated for colorimetric determination of glucose and cholesterol in human serum. Cu-CuFe 2 O 4 @L-Arg NPs with superior peroxidase-like activities were synthesized via a straightforward hydrothermal approach. The accelerated electron transfer process of Cu 0 and its role as an electron donor contributed to excellent catalytic activity of Cu-CuFe 2 O 4 @L-Arg toward 3,3’,5,5’-tetramethylbenzidine (TMB). Portable kits include glucose oxidase or cholesterol oxidase, phosphate buffer solution (PBS) pH 7.0, Cu-CuFe 2 O 4 @L-Arg, PBS pH 4.0 and TMB. Among the enzyme-like reactions, Cu-CuFe 2 O 4 @L-Arg catalyzes the conversion of H 2 O 2 into hydroxyl radicals (•OH), which subsequently oxidize the TMB substrate to yield a blue-colored product. The signal intensities of the blue products were proportional to H 2 O 2 , which was produced in the cholesterol or glucose systems. This method achieves linear detection of free cholesterol from 5 µM to 1.2 mM, exhibiting a low LOD of 1 µM, and was validated by obtaining recoveries of 97-103.5% for total cholesterol in human serum analyses. The method enabled the linear quantification of glucose from 50 to 1500 µM with a limit of detection (LOD) of 5 µM, and recoveries in human serum ranged from 96% to 102.8%. The portable kits, characterized by low cost, visual readout, high specificity, and long-term stability, demonstrate considerable potential for self-monitoring devices for key clinical biomarkers. Graphical
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MOLECULAR PHARMACEUTICS
·
2021
·
IF 4.939
Zhiting Sun,Xiaoxiao Wang,Jing Liu,Zhihong Wang,Weiwei Wang,Deling Kong,Xigang Leng
Photodynamic therapy (PDT) is promising for clinical cancer therapy; however, the efficacy was limited as an individual treatment regimen. Here, an approach synergistically combining PDT and nitric oxide (NO) gas therapy along with destruction of the tumor extracellular matrix (ECM) was presented to eliminate cancer. Specifically, the NO donor l-arginine (l-Arg) and the photosensitizer indocyanine green (ICG) were co-encapsulated in poly(lactic-glycolic acid) (PLGA) nanoparticles and then loaded into the poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL–PEG–PCL) hydrogel to develop an injectable, thermosensitive dual drug delivery system (PLGA@ICG@l-Arg/Gel). Significantly, reactive oxygen species (ROS) produced by PLGA@ICG@l-Arg/Gel under near-infrared (NIR) light irradiation could not only result in the apoptosis of cancer cells but also oxidize l-Arg to generate NO, which could suppress the proliferation of cancer cells. Moreover, ROS could further oxidize NO to generate peroxynitrite anions (ONOO–). ONOO– could activate matrix metalloproteinases (MMPs), which notably degraded collagen in ECM so as to damage the tumor microenvironment. PLGA@ICG@l-Arg/Gel significantly increased the antitumor efficacy against highly malignant 4T1 tumors in mice. Taken together, PLGA@ICG@l-Arg/Gel is a multifunctional platform that provides a novel strategy for cancer treatment with cascade amplification of the ROS oxidation effect, which holds great potential in clinical translation.
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NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 23
·14486-14497
·
IF 3.269
Yuxian Wang,Ling Jiang,Ce Wei,Hongman Zhang
Fatty acids, composed of an amphiphilic carbon chain and a carboxyl group, are a simple class of anion surfactants when they are protonated in the presence of an alkali. They can form ion-pairing amphiphile molecules and self-assemble into different nanostructures, which can be widely applied. However, the phase transition of these nanostructures can be easily triggered by external conditions, such as pH, temperature, the addition of salts and so on. In this work, medium-chain fatty acids (MCFAs) containing 8–12 carbons were chosen as the objects for self-assembly with organic amino counter-ions, L-arginine. We investigated the effects of chain length, pH, temperature and solvent on the phase transition based on diagrams, which could be determined by freeze fracture transmission electron microscopy (FF-TEM) and polarizing microscopy observations (PMO) combined with rheological properties. The results showed that rich phase behavior was observed in the octanoic acid (C8:0) system. However, it was insensitive to changes in pH because of the lower hydrophobicity. In contrast, for decanoic acid (C10:0) and lauric acid (C12:0) systems, phase transition from the L1 phase (micelles) to the Lα phase (vesicles) was observed and they showed stimulus-response to the change of pH. At the same time, lauric acid was sensitive to temperature, which was ascribed to the higher Krafft point. Besides, when the solvent was replaced by glycerin, phase transitions occurred from vesicles to micelles in these three systems. We hope that this phase transition observed in the MCFA system will provide a good insight into drug delivery, material science, and other related areas.
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ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Zihan Zhao,Chunfeng Li,Lei Le,Mingli Liu,Zhihao Zhang
The expanding application of corn stalk/polypropylene composites (CPCs) is accompanied by increasingly stringent performance requirements. Consequently, endowing flame retardancy, smoke suppression, and multifunctionality to CPCs while maintaining their mechanical properties is significant. Herein, a biobased flame retardant, arginine/ZIF-67 dual-modified ammonium polyphosphate (APP-Arg-MOF; MOF, metal–organic framework), for CPCs was engineered. APP was first functionalized with Arg via ion exchange to enhance its compatibility and flame retardancy. Subsequently, ZIF-67 was uniformly grown on APP-Arg through coordination-driven assembly using cobalt ions and 2-methylimidazole. The experimental results explained the exceptional multifunctional enhancement. The CPC/APP-Arg-MOF composite achieved a limiting oxygen index of 29.2% and passed the UL-94 V-0 rating. Cone calorimetry revealed significant reductions in the peak heat release rate (63.1%), total heat release (21.9%), and total smoke production (34.7%). Remarkably, the bending and tensile strengths increased by 10.0% and 26.9%, respectively, relative to CPC/APP, attributed to improved interfacial compatibility via Arg and ZIF-67 nanostructures. Furthermore, the CPC/APP-Arg-MOF composite exhibited potent antimicrobial activity, with inhibition rates of 97% against E. coli and 99% against S. aureus, leveraging synergistic effects of Arg and Co2+ release from ZIF-67. Meanwhile, the composite also maintained flame-retardant and antibacterial properties after 200 h of UV aging. Therefore, this work presents a sustainable APP surface modification strategy by utilizing Arg and ZIF-67 to simultaneously enhance fire safety, smoke suppression, and mechanical, antimicrobial, and anti-UV aging performances of CPC, offering significant potential for CPC applications.
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危险品化学品经营许可证（带存储）